A probiotic chinese herbal medicine compound composition, its preparation method and application

Abstract

The application discloses a probiotic Chinese herbal medicine compound composition, which comprises a Chinese herbal medicine compound and probiotics; wherein the Chinese herbal medicine compound comprises lotus leaf extract, hawthorn extract and kuding tea extract; and the probiotics are bifidobacterium.The application also discloses a preparation method of the probiotic Chinese herbal medicine compound composition and application of the probiotic Chinese herbal medicine compound composition in preparation of a fat-reducing product.The probiotic Chinese herbal medicine compound composition provided by the application not only has a significant improvement effect on fat accumulation, blood lipid and liver metabolism disorder caused by high-fat diet, but also has a significant regulation effect on genes related to liver sterol metabolism and sterol biosynthesis and a steroid biosynthesis pathway.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of health food, in particular to a probiotic Chinese herbal compound composition for reducing fat and a preparation method and application thereof. BACKGROUND

[0002] With the change of modern lifestyle and dietary structure, obesity and its accompanying metabolic disorders are becoming increasingly common worldwide, and have become an important public health problem that seriously threatens human health. Obesity not only manifests as an increase in body weight, but also is accompanied by abnormal accumulation of visceral fat, significant increase in blood lipid levels, and disorder of liver lipid metabolism, thereby inducing metabolic diseases such as hypertension, hyperglycemia, and hyperlipidemia, and even being closely related to the occurrence of chronic diseases such as diabetes and atherosclerosis. These diseases can significantly increase the mortality and life burden of the patients, and thus the control of obesity and its related metabolic disorders has become a research focus in clinical medicine and nutritional science.

[0003] Probiotics have been widely used to improve lipid metabolism due to their effects on regulating intestinal flora and promoting metabolic health. Studies have shown that specific probiotics can alleviate obesity-related symptoms to a certain extent by affecting cholesterol metabolism and lipid absorption. However, the effectiveness of probiotics depends on their survival rate and activity in the intestinal tract, and it is necessary to ensure that a sufficient number of active probiotics can achieve the desired metabolic regulation effect. Generally, the number of active probiotics in functional foods should not be less than 10 6 CFU / mL to ensure their smooth passage through the gastrointestinal tract and exert their efficacy in the intestine. However, the number and activity of probiotics are easily affected during product preparation and storage, and therefore single probiotic products often face limitations in achieving fat-reducing effects.

[0004] Chinese herbal compound conditioning has played an important role in traditional Chinese medicine since ancient times, and has unique application value in blood lipid regulation and body fat management. Modern pharmacological studies have confirmed that many Chinese herbal ingredients can positively affect lipid metabolism through multi-target mechanisms, thereby exerting lipid-lowering, antioxidant, anti-inflammatory, and other effects. By compounding probiotics with Chinese herbal medicines to form a synergistic composition, the intestinal flora and host lipid metabolism can be comprehensively regulated, and this method has great application potential and market value. In particular, based on lotus leaf, hawthorn, and kuding tea, which have lipid-lowering potential, their compounding with specific probiotics can provide an efficient solution for fat reduction and metabolic regulation.

[0005] However, there are still many technical problems to be solved in the development of Chinese herbal medicine probiotic compositions for weight loss. For example, the optimal combination of probiotics and Chinese herbal medicines for efficient weight loss, the reasonable dosage ratio, and the synergistic mechanism of active ingredients are not clear. At the same time, how to ensure the stability and efficacy of active ingredients in product preparation and application is still a major challenge in research and development. Therefore, developing a probiotic Chinese herbal medicine compound composition with reasonable formula, significant efficacy, and clear mechanism can provide a scientific solution for metabolic disorders and fat accumulation caused by high-fat diet, which is a problem that needs to be solved by technical personnel in the field. SUMMARY

[0006] The technical problem to be solved by the present application is to provide a probiotic Chinese herbal medicine compound composition which not only has a significant improvement effect on fat accumulation, blood lipid and liver metabolic disorders caused by high-fat diet, but also has a significant regulating effect on genes related to liver sterol metabolism and sterol biosynthesis and steroid biosynthesis pathway.

[0007] To solve the above technical problems, the present application provides the following technical solutions:

[0008] The first aspect of the present application provides a probiotic Chinese herbal medicine compound composition comprising a Chinese herbal medicine compound and probiotics; wherein the Chinese herbal medicine compound comprises lotus leaf extract, hawthorn extract and kuding tea extract; and the probiotics are bifidobacterium.

[0009] Lotus leaf contains a variety of alkaloids such as lotus base, proto-norlotus leaf base, lotus leaf base and flavonoids, and vitamin C, which is a traditional Chinese medicine for lowering blood lipid and weight loss, and has the effects of clearing heat, diuresis, clearing lipid, and defecation.

[0010] Kuding tea is a traditional pure natural health beverage in China. Kuding tea contains kuding saponin, amino acid, vitamin C, polyphenol, flavonoid, caffeine, protein and other components. It has the effects of clearing heat and relieving summer-heat, diuresis and strengthening heart, moistening throat and relieving cough, reducing blood pressure and weight loss, and promoting blood circulation.

[0011] Hawthorn contains flavonoids, citric acid, malic acid, ascorbic acid and carbohydrates, and has the effects of lowering blood pressure and promoting gastrointestinal fat excretion. Traditional Chinese medicine believes that hawthorn can invigorate the spleen and eliminate stagnation, which is beneficial to weight loss and can be used to treat obesity.

[0012] Bifidobacterium can promote fat metabolism and reduce fat accumulation through multiple pathways. It can promote fat metabolism by improving intestinal microecological balance, inhibiting the growth of harmful bacteria, and reducing intestinal inflammation; it can help control weight and reduce fat accumulation by improving insulin sensitivity and regulating insulin receptors and insulin signaling pathways; it can promote the oxidation of short-chain fatty acids (such as acetic acid, propionic acid, and butyric acid) and inhibit fat cell accumulation by regulating fatty acid metabolism; in addition, bifidobacterium can also reduce chronic low-grade inflammation in adipose tissue, reduce fat synthesis and adipocyte differentiation, regulate intestinal barrier function, enhance fatty acid oxidation, and improve blood glucose levels. These effects jointly act on fat metabolism to produce a fat-reducing effect, with the main targets including PPARs, AMPK, SCFA receptors, and genes related to fatty acid synthesis.

[0013] The inventors found that the Chinese herbal compound, lotus leaf extract, hawthorn extract, and kuding tea extract, when used in combination with bifidobacterium, can significantly improve their fat-reducing efficacy, not only significantly improving the fat accumulation, blood lipids, and liver metabolic disorders caused by high-fat diet, but also significantly regulating the genes related to liver sterol metabolism and sterol biosynthesis and the steroid biosynthesis pathway.

[0014] Specifically, the probiotic Chinese herbal compound composition of the present application significantly reduces the weight of epididymal fat, mesenteric fat, and inguinal fat, significantly improves the decrease in serum high-density lipoprotein cholesterol (HDLC) level and glutathione peroxidase (GPX) activity caused by high-fat diet, and significantly reduces the content of triglyceride (TG) and total cholesterol (TC) in the liver. The results of liver morphological observation show that the probiotic Chinese herbal compound composition of the present application significantly reduces the number and size of lipid droplets in the liver, has a certain improvement effect on the excessive deposition of liver fat caused by high-fat diet; and significantly reduces the volume of epididymal adipocytes.

[0015] Further, the Chinese herbal compound includes the following components by mass percentage: lotus leaf extract 1-10%, hawthorn extract 80-98.7%, and kuding tea extract 0.1-5%. Preferably, the mass ratio of lotus leaf extract, hawthorn extract, and kuding tea extract in the Chinese herbal composition is 3:24:2.

[0016] In the present application, the probiotic can be one or more of Bifidobacterium bifidum, Bifidobacterium lactis, Bifidobacterium longum, and Bifidobacterium breve.

[0017] In the present application, the probiotic in the composition is bifidobacterium powder. Further, the bifidobacterium powder is self-prepared bifidobacterium freeze-dried powder with a bacterial content of 1x10 10CFU / g. The present application does not make specific restrictions on the source of probiotics, and one or more of the commonly used bifidobacterium strains on the market or self-prepared bifidobacterium strains can be used according to the needs, and the strain source does not affect the implementation effect of the present application.

[0018] Further, in the probiotic Chinese herbal compound composition, the mass ratio of the Chinese herbal compound to the probiotic is 72-90% of the Chinese herbal compound and 10-28% of the probiotic. For example, the mass percentage of the Chinese herbal compound in the probiotic Chinese herbal compound composition can be 72%, 75%, 78%, 80%, 82%, 85%, 88%, 90%, etc., and the mass percentage of the probiotic can be 10%, 12%, 15%, 18%, 20%, 22%, 25%, 28%, etc.

[0019] The second aspect of the present application provides a preparation method of the probiotic Chinese herbal compound composition, comprising the following steps:

[0020] S1. The extract raw material is extracted with water twice, and impurities are filtered out after each extraction to obtain an extract solution; the extract raw material is lotus leaf powder, hawthorn powder or kuding tea powder;

[0021] S2. The extract solution is concentrated under vacuum conditions to a relative density of 1.09-1.12 g / cm 3 , and then malt dextrin is added and high-pressure sterilization is performed;

[0022] S3. In a clean environment, the sterilized extract solution is spray dried to obtain a powdered material; after crushing, sieving and mixing, an extract powder is obtained;

[0023] S4. The extract powder is mixed with probiotic powder to obtain the probiotic Chinese herbal compound composition.

[0024] Further, in step S1, when the extract raw material is lotus leaf powder, the lotus leaf powder is soaked with 30 times and 20 times water respectively, and the extraction time is 120 minutes and 60 minutes respectively;

[0025] When the extract raw material is hawthorn powder, the hawthorn powder is soaked with 12 times and 8 times water respectively, and the extraction time is 120 minutes and 60 minutes respectively;

[0026] When the extract raw material is kuding tea powder, the kuding tea powder is soaked with 12 times and 8 times water respectively, and the extraction time is 90 minutes and 60 minutes respectively.

[0027] Further, in step S2, the vacuum degree is controlled to be-0.06 to-0.09 MPa and the concentration temperature is 50-75°C during concentration; and the temperature is controlled to be 115-135°C during high-pressure sterilization.

[0028] Further, in step S3, the inlet air temperature is 180-205℃ and the outlet air temperature is 85-105℃ during spray drying.

[0029] In some embodiments of the present application, taking lotus leaf extract as an example, the specific extraction process is as follows:

[0030] a1. Raw material source and detection: QC detection confirms the lotus leaf powder raw material, and prepares the water extraction process.

[0031] a2. Extraction and filtration: water extraction is used twice, 30 times and 20 times of water are used to soak the lotus leaf powder, the extraction time is 120 minutes and 60 minutes, and filtration is performed after each extraction to remove insoluble impurities.

[0032] a3. Concentration and sterilization: the filtered extract is concentrated under vacuum conditions, the vacuum degree is controlled to be-0.06 to-0.09 MPa, the concentration temperature is 50-75℃, and the final concentrated liquid relative density is 1.09-1.12. Malt dextrin is added to the concentrated liquid, and high-pressure sterilization is performed, with the temperature kept at 115-135℃.

[0033] a4. Drying and crushing: the sterilized extract is spray dried in a clean production area (D level), the inlet air temperature is 180-205℃, and the outlet air temperature is 85-105℃. After drying, the powder material is crushed and passed through a 100 mesh screen.

[0034] a5. Screening, mixing and detection: under the control of CCP standards, the powder is screened again through a 100 mesh screen, mixed for 50-60 minutes. The obtained lotus leaf powder extract is subjected to internal control detection, sealed and packaged, and subjected to CCP inspection to confirm that it is qualified before being subjected to external packaging and sent to the warehouse for storage for future use.

[0035] The third aspect of the present application provides the use of the above-mentioned probiotic Chinese herbal compound composition in the preparation of a fat-reducing product.

[0036] Further, the fat-reducing product is used to improve fat accumulation, blood lipids and liver metabolic disorders caused by high-fat diet.

[0037] Further, the fat-reducing product is used to regulate genes related to hepatic sterol metabolism and sterol biosynthesis and the steroid biosynthesis pathway.

[0038] Further, the use dosage of the probiotic Chinese herbal compound composition is: the taking amount of the Chinese herbal compound is 10-24 g / d, for example, it can be 10 g / d, 12 g / d, 14 g / d, 16 g / d, 18 g / d, 20 g / d, 22 g / d, 24 g / d, etc. The taking amount of the probiotic is 1-6 g / d, for example, it can be 1 g / d, 2 g / d, 3 g / d, 4 g / d, 5 g / d, 6 g / d, etc.

[0039] In determining the taking amount of the probiotic, the final taking amount can be adjusted within the range according to the differences in individual body weight and required intake amount of the probiotic to meet specific health needs. Preferably, the taking amount of the probiotic is 2 g / d.

[0040] Further, the fat-reducing product includes but is not limited to fat-reducing drugs, fat-reducing foods and fat-reducing health products.

[0041] Compared with the prior art, the present application has the following beneficial effects:

[0042] 1. The probiotic Chinese herbal compound composition provided by the present application has a significant improvement effect on fat accumulation, blood lipid and liver metabolism disorder caused by high-fat diet. The probiotic Chinese herbal compound composition significantly reduces the weights of epididymal fat, mesenteric fat and inguinal fat, significantly improves the decrease of serum high-density lipoprotein cholesterol (HDLC) level and glutathione peroxidase (GPX) activity caused by high-fat diet, and can significantly reduce the contents of triglyceride (TG) and total cholesterol (TC) in the liver. In addition, the morphological observation results show that the probiotic Chinese herbal compound composition significantly reduces the number and size of lipid droplets in the liver and significantly reduces the volume of epididymal adipocytes.

[0043] 2. The probiotic Chinese herbal compound composition provided by the present application has a significant regulation effect on genes related to liver sterol metabolism and sterol biosynthesis and the steroid biosynthesis pathway.

[0044] 3. The probiotic Chinese herbal compound composition provided by the present application can be used for preparing various fat-reducing foods and fat-reducing health products. BRIEF DESCRIPTION OF DRAWINGS

[0045] Figure 1 A column chart for the effect of the probiotic Chinese herbal compound composition on the weight of epididymal fat of mice;

[0046] Figure 2 A column chart for the effect of the probiotic Chinese herbal compound composition on the weight of mesenteric fat of mice;

[0047] Figure 3 A column chart for the effect of the probiotic Chinese herbal compound composition on the weight of inguinal fat of mice;

[0048] Figure 4 Bar chart for the effect of the probiotic Chinese herbal compound composition on the level of serum high-density lipoprotein cholesterol (HDLC) in mice;

[0049] Figure 5 Bar chart for the effect of the probiotic Chinese herbal compound composition on the content of triglyceride (TG) in the liver;

[0050] Figure 6 Bar chart for the effect of the probiotic Chinese herbal compound composition on the content of total cholesterol (TC) in the liver;

[0051] Figure 7 Bar chart for the effect of the probiotic Chinese herbal compound composition on the glutathione peroxidase activity (GPX) in the liver;

[0052] Figure 8 HE staining photos for the effect of the probiotic Chinese herbal compound composition on the morphology of liver cells;

[0053] Figure 9 HE staining photos for the effect of the probiotic Chinese herbal compound composition on the morphology of epididymal adipocytes;

[0054] Figure 10 Volcano plot for the differentially expressed genes in the liver under the effect of the probiotic Chinese herbal compound composition;

[0055] Figure 11 Bar chart for the GO enrichment analysis of the differentially expressed genes in the liver under the effect of the probiotic Chinese herbal compound composition;

[0056] Figure 12 Scatter plot for the KEGG enrichment analysis of the differentially expressed genes in the liver under the effect of the probiotic Chinese herbal compound composition. DETAILED DESCRIPTION

[0057] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the embodiments of the present application and the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0058] Embodiment 1

[0059] The present embodiment provides a probiotic Chinese herbal compound composition, comprising a Chinese herbal compound (TCM compound) and probiotics;

[0060] The Chinese herbal medicine compound comprises lotus leaf extract, hawthorn extract and kuding tea extract, and the mass ratio of the three is 3:24:2. The probiotic is freeze-dried powder of Bifidobacterium bifidum, and the bacterial content is 1*10 10 CFU / g.

[0061] Comparative Example 1

[0062] The present comparative example provides a Chinese herbal medicine compound (TCM compound) comprising lotus leaf extract, hawthorn extract and kuding tea extract, and the mass ratio of the three is 3:24:2.

[0063] Comparative Example 2

[0064] The present comparative example provides a probiotic freeze-dried powder, which is freeze-dried powder of Bifidobacterium bifidum, and the bacterial content is 1*10 10 CFU / g.

[0065] Animal feeding experiment

[0066] 6-week-old male C57BL / 6 mice were raised in a SPF-grade rodent breeding room, and the feeding environment temperature was controlled at 22±2℃, with 12h light-dark cycle and relative humidity of 40-60%. After adaptive feeding for 1 week (7 days), the experimental mice were randomly divided into 7 experimental groups (a total of 7 groups, 10 mice in each group), and each group was fed with different processed diets. During the experiment, the mice could freely eat and drink water, and were fed for 8 weeks (56 days). According to the body surface area coefficient, the dose of mouse (0.020 kg) animal experiment was converted to human (60.0 kg) dose of about 1 / 268.1, so the total dose of each experimental group was controlled at 10 mg / d / pcs.

[0067] The specific experimental grouping design is as follows:

[0068] (1) ordinary diet group, fed with ordinary mouse basic feed with fat energy supply ratio of 10%, denoted as Chow;

[0069] (2) high-fat diet group, fed with purified high-fat feed with fat energy supply ratio of 60%, denoted as HFD;

[0070] (3) high-fat diet + probiotic group, fed with purified high-fat feed (fat energy supply ratio 60%), and the probiotic Bifidobacterium freeze-dried powder of Comparative Example 2 was administered intragastrically at a dose of 10 mg / d / pcs, denoted as HFD+probiotic;

[0071] (4) high-fat diet + TCM compound group, fed with purified high-fat feed added with the TCM compound of Comparative Example 1 (fat energy supply ratio 60%, TCM compound addition amount 0.38 g / 100 g, equivalent to 10 mg / d / pcs per mouse), denoted as HFD+TCM;

[0072] (5) High-fat diet + TCM compound + probiotic group: The group was fed a purified high-fat diet with added TCM compound of Example 1 (fat energy ratio of 60%, TCM compound added amount of 0.33g / 100g - equivalent to 8.8mg / d / pcs per animal); a quantitative amount of probiotics of Example 1 was administered by gavage every day (at a dosage of 1.2mg / d / pcs), which was recorded as HFD+TCM+probiotics.

[0073] Adipose tissue assessment procedure

[0074] To assess the weight of fat reserves (including epididymal, mesenteric, and inguinal fat) in each group of mice, the following procedures were performed:

[0075] (1) Experimental preparation: Disinfect and calibrate all dissection tools and mark the adipose tissue collection tube (EP tube).

[0076] (2) Euthanasia and dissection: Euthanasia was performed in accordance with the ethical standards for laboratory animals. The mice were placed in a supine position and cut along the midline of the abdomen.

[0077] (3) Separation of epididymal adipose tissue: Gently pull back into the intestine, locate and separate the fat around the epididymis.

[0078] (4) Separation of mesenteric adipose tissue: Locate the fat around the mesentery and carefully remove it.

[0079] (5) Inguinal adipose tissue separation: enter the groin from the subcutaneous tissue to obtain fat from the groin area.

[0080] (6) Weighing and recording: Transfer each adipose tissue to a pre-weighing tube, record the weight using a high-precision balance, and repeat the weighing to obtain the precise weight of each mouse adipose tissue.

[0081] (7) This experiment systematically analyzed the effects of each treatment group on the lipid metabolism of mice through precise fat weighing data, in order to evaluate the efficacy and mechanism of the combined application of traditional Chinese medicine compound and probiotics.

[0082] 1. Effects of probiotic-herbal compound on the weight of epididymal fat, mesenteric fat, and inguinal fat.

[0083] Figure 1 A bar chart showing the effect of a probiotic-based herbal formula on epididymal fat weight in mice. From... Figure 1 The results show that the epididymal fat weight of mice was low when fed a normal diet. However, the epididymal fat weight of mice increased significantly when fed a high-fat diet. Adding the traditional Chinese medicine compound TCM to the high-fat diet, or administering the probiotic Bifidobacterium via gavage daily, significantly reduced the epididymal fat weight of mice. This indicates that both Bifidobacterium and the traditional Chinese medicine compound TCM are beneficial in reducing the epididymal fat weight of mice.

[0084] Among the other groups, the group of HFD+TCM+probiotics had the most significant effect on reducing epididymal fat weight. Specifically, the epididymal fat weight of mice was reduced from 31.47 mg / g BW to 17.64 mg / g BW, with a reduction percentage of 43.94% compared to the HFD group.

[0085] Figure 2 The bar chart for the effect of probiotic Chinese herbal compound on the weight of the mesenteric fat of mice is shown in FIG. 6. As can be seen from FIG. 6, the mesenteric fat weight of mice was low when fed with the normal diet. The mesenteric fat weight of mice increased significantly when fed with the high-fat diet. The mesenteric fat weight of mice decreased significantly when fed with the high-fat diet supplemented with the Chinese herbal compound TCM or when fed with the high-fat diet supplemented with probiotic Bifidobacterium, which indicates that both Bifidobacterium and the Chinese herbal compound TCM are beneficial to reducing the mesenteric fat weight of mice. Figure 2

[0086] Among the other groups, the group of HFD+TCM+probiotics had the most significant effect on reducing mesenteric fat weight. Specifically, the mesenteric fat weight of mice was reduced from 8.24 mg / g BW to 4.25 mg / g BW, with a reduction percentage of 48.42% compared to the HFD group.

[0087] Figure 3 The bar chart for the effect of probiotic Chinese herbal compound on the weight of the inguinal fat of mice is shown in FIG. 8. As can be seen from FIG. 8, the inguinal fat weight of mice was low when fed with the normal diet. The inguinal fat weight of mice increased significantly when fed with the high-fat diet. The inguinal fat weight of mice decreased significantly when fed with the high-fat diet supplemented with the Chinese herbal compound TCM or when fed with the high-fat diet supplemented with probiotic Bifidobacterium, which indicates that both Bifidobacterium and the Chinese herbal compound TCM are beneficial to reducing the inguinal fat weight of mice. Figure 3

[0088] Among the other groups, the group of HFD+TCM+probiotics had the most significant effect on reducing inguinal fat weight. Specifically, the inguinal fat weight of mice was reduced from 26.17 mg / g BW to 11.00 mg / g BW, with a reduction percentage of 57.99% compared to the HFD group.

[0089] 2. Effect of probiotic Chinese herbal compound on serum high-density lipoprotein cholesterol (HDLC) level ​​

[0090] Figure 4 The bar chart for the effect of probiotic Chinese herbal compound on the level of serum high-density lipoprotein cholesterol (HDLC) of mice. The decrease of HDLC level is an important feature of abnormal blood lipid metabolism. As can be seen from the figure, the HDLC level of mice is about 2.9 mmol / L when fed with ordinary diet; the HDLC of mice has a significant decrease when fed with high-fat diet. The HDLC of mice has a significant increase when the high-fat feed is added with Chinese herbal compound TCM or when probiotic Bifidobacterium is administered by gavage every day, which shows that both Bifidobacterium and Chinese herbal compound TCM are beneficial to the increase of HDLC of mice.

[0091] Compared with other groups, the HFD+TCM+probiotic group has the most significant effect on the increase of HDLC of mice. Specifically, the HDLC of mice increases from 1.67 mmol / L to 4.12 mmol / L, with a percentage increase of 146.71% compared with the HFD group.

[0092] 3. Effect of probiotic Chinese herbal compound on the content of triglyceride (TG) in liver

[0093] Figure 5 The bar chart for the effect of probiotic Chinese herbal compound on the content of triglyceride (TG) in liver. As can be seen from the figure, the content of triglyceride in liver of mice is low when fed with ordinary diet. The content of triglyceride in liver of mice has a significant increase when fed with high-fat diet. The content of triglyceride in liver of mice does not decrease when the high-fat feed is added with Chinese herbal compound TCM or when probiotic Bifidobacterium is administered by gavage every day, which shows that the use of Bifidobacterium or Chinese herbal compound TCM alone does not decrease the content of triglyceride in liver of mice. Figure 5

[0094] Compared with other groups, the HFD+TCM+probiotic group has a significant decrease in the content of triglyceride in liver of mice. Specifically, the content of triglyceride (TG) in liver of mice decreases from 0.86 mmol / L to 0.50 mmol / L, with a percentage decrease of 41.7% compared with the HFD group. This shows that the combined use of Bifidobacterium and Chinese herbal compound TCM can significantly decrease the content of triglyceride in liver of mice.

[0095] 4. Effect of probiotic Chinese herbal compound on the content of total cholesterol (TC) in liver

[0096] Figure 6 The bar chart for the effect of probiotic Chinese herbal compound on the content of total cholesterol (TC) in liver. As can be seen from the figure, the content of total cholesterol in liver of mice is low when fed with ordinary diet. The content of total cholesterol in liver of mice has a significant increase when fed with high-fat diet. The content of total cholesterol in liver of mice does not decrease when the high-fat feed is added with Chinese herbal compound TCM or when probiotic Bifidobacterium is administered by gavage every day, which shows that the use of Bifidobacterium or Chinese herbal compound TCM alone does not decrease the content of total cholesterol in liver of mice.​Figure 6 The results show that when mice were fed a normal diet, the total cholesterol content in their livers was low. However, when fed a high-fat diet, the total cholesterol content in their livers increased significantly. Daily gavage administration of the probiotic Bifidobacterium to the high-fat diet resulted in a slight decrease in the total cholesterol content in the mouse livers. Furthermore, adding the traditional Chinese medicine compound TCM to the high-fat diet led to a more significant decrease in the total cholesterol content in the mouse livers. This indicates that, compared to probiotics, the traditional Chinese medicine compound TCM is more effective in reducing the total cholesterol content in mouse livers.

[0097] Compared to the other groups, the HFD+TCM+probiotic group, which was fed a high-fat diet supplemented with TCM (a traditional Chinese medicine compound) and administered Bifidobacterium via gavage daily, showed the most significant decrease in total cholesterol content in the liver of mice. Specifically, compared to the HFD group, the total cholesterol content in the liver of mice decreased from 0.15 mmol / L to 0.07 mmol / L, a reduction of 54.2%.

[0098] 5. Effects of probiotic-herbal compound on glutathione peroxidase (GPX) activity in the liver.

[0099] Figure 7 This is a bar chart showing the effect of probiotic-based traditional Chinese medicine compound on glutathione peroxidase (GPX) activity in the liver. From... Figure 7 The results show that GPX activity in mouse livers was high when fed a normal diet. However, GPX activity decreased when fed a high-fat diet. Daily gavage administration of the probiotic Bifidobacterium to the high-fat diet resulted in a slight increase in GPX activity in the mouse livers. Furthermore, the addition of the traditional Chinese medicine compound TCM to the high-fat diet significantly increased GPX activity in the mouse livers. This indicates that, compared to probiotics, the traditional Chinese medicine compound TCM is more effective in improving GPX activity in mouse livers.

[0100] Compared to the other groups, the HFD+TCM+probiotic group showed the most significant increase in GPX activity in the liver of mice fed a high-fat diet supplemented with TCM (a traditional Chinese medicine compound) and administered daily via gavage with Bifidobacterium. Specifically, compared to the HFD group, GPX activity in the liver of mice increased from 5.43 U / mg to 28.33 U / mg, an increase of 421.73%.

[0101] 6. Effects of probiotic-herbal compound on mouse liver cell morphology

[0102] Figure 8This image shows an HE staining image of the effects of a probiotic-TCM compound on liver cell morphology. The HE staining results in the image show that liver cells in the Chow group mice were tightly packed, while the livers of the HFD group mice exhibited significant vacuolation. The white vacuoles in the sections represent lipid droplets deposited in the liver. The probiotic and TCM compound groups significantly reduced the number and size of lipid droplets, showing a certain ameliorative effect on excessive liver fat deposition induced by a high-fat diet. The high-fat diet + TCM compound + probiotic group showed the most significant improvement.

[0103] 7. Effects of probiotic-herbal compound on the morphology of epididymal adipocytes in mice

[0104] Figure 9 HE staining images show the effects of probiotics and traditional Chinese medicine compound on the morphology of epididymal adipocytes. The HE staining results in the images show that the epididymal adipocytes in the Chow group were small, clearly morphologically distinct, and structurally intact; in contrast, the epididymal adipocytes in the HFD group were significantly larger. The addition of probiotics and TCM compound significantly reduced the volume of epididymal adipocytes, and the number of cells observed under the same magnification was significantly increased. The high-fat diet + TCM compound + probiotics group showed the most significant effect in improving epididymal adipocyte hypertrophy.

[0105] 8. Effects of probiotic-herbal compound on gene expression in mouse liver

[0106] Figure 10 This is a volcano plot showing the differentially expressed genes in the liver by a probiotic-TCM compound. Transcriptomic analysis of mouse livers in the figure shows that, compared to the HFD group, the high-fat diet + TCM compound + probiotic group had 647 differentially expressed genes, including 324 upregulated genes and 323 downregulated genes.

[0107] Figure 11 GO enrichment analysis results showed that differentially expressed genes were concentrated in secondary alcohol biosynthesis, cholesterol biosynthesis, and sterol biosynthesis.

[0108] Figure 12 KEGG enrichment analysis results showed that differentially expressed genes are mainly involved in pathways related to terpene skeleton biosynthesis, circadian rhythm, bile secretion, and retinol metabolism.

[0109] In summary, the probiotic herbal compound composition provided by this invention not only has a significant effect on improving fat accumulation, blood lipids and liver metabolic disorders caused by a high-fat diet, but also has a significant regulatory effect on liver sterol metabolism and sterol biosynthesis-related genes and steroid biosynthesis pathways. It can be used to prepare various fat-reducing drugs, fat-reducing foods and fat-reducing health products.

[0110] The above-described embodiments are merely preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Any equivalent substitutions or transformations made by those skilled in the art based on the present application are within the protection scope of the present application. The protection scope of the present application is subject to the claims.

Claims

1. Use of a probiotic Chinese herbal compound composition in the manufacture of a product for improving the symptoms of, The symptoms include: (1) decrease of glutathione peroxidase activity caused by high-fat diet; (2) increase of triglyceride content and total cholesterol content in liver caused by high-fat diet; The probiotic Chinese herbal compound composition is prepared from a Chinese herbal compound and probiotics; the Chinese herbal compound is a combination of lotus leaf extract, hawthorn extract and kuding tea extract; the mass ratio of the lotus leaf extract, the hawthorn extract and the kuding tea extract in the Chinese herbal compound is 3:24:2; the probiotics are bifidobacterium; the mass percentage of the Chinese herbal compound and the probiotics in the probiotic Chinese herbal compound composition is 88% and 12%, respectively; The product is a drug or a health food; The probiotic is Bifidobacterium powder with a bacterial content of 1*10 10 CFU / g; The preparation method of the probiotic Chinese herbal compound composition comprises the following steps: S1. The extract raw material is extracted with water twice, and impurities are filtered out after each extraction to obtain an extract solution; the extract raw material is lotus leaf powder, hawthorn powder or kuding tea powder; S2. The extract is concentrated under vacuum to a relative density of 1.09-1.12 g / cm 3 then maltodextrin is added and autoclaved; S3. The sterilized extract solution is spray dried in a clean environment to obtain a powdery material; the powdery material is crushed, sieved and mixed to obtain an extract powder; S4. The extract powder is mixed with probiotic powder to obtain the probiotic Chinese herbal compound composition.

2. Use according to claim 1, characterized in that, In step S1, when the extract raw material is lotus leaf powder, the lotus leaf powder is soaked with 30 times and 20 times of water, respectively, and the extraction time is 120 minutes and 60 minutes, respectively; When the extract raw material is hawthorn powder, the hawthorn powder is soaked with 12 times and 8 times of water, respectively, and the extraction time is 120 minutes and 60 minutes, respectively; When the extract raw material is kuding tea powder, the kuding tea powder is soaked with 12 times and 8 times of water, respectively, and the extraction time is 90 minutes and 60 minutes, respectively.

3. Use according to claim 1, characterized in that, In step S2, the vacuum degree is controlled to be -0.06 to -0.09 MPa and the concentration temperature is 50-75℃ during concentration; the temperature of the autoclaving is controlled to be 115-135℃; In step S3, the inlet air temperature is 180-205℃ and the outlet air temperature is 85-105℃ during spray drying.

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