Self-assembling emulsifier, glabridin liposome containing the self-assembling emulsifier, and preparation method and application thereof

Abstract

The invention discloses a self-assembling emulsifier, comprising emulsifier A, emulsifier B and emulsifier C; the mass ratio thereof is (0.05-3):(0.05-1):(0.05-3); emulsifier A comprises at least one of PPG-24-glycerol polyether-24, PPG-decyl tetradecanol polyether-24, and di(PPG-2 myristyl alcohol polyether-10) adipate; emulsifier B comprises at least one of polyquaternium-52, polyquaternium-61, polyquaternium-74, and polyquaternium-37; emulsifier C comprises at least one of PEG-40 hydrogenated castor oil, PEG-80 sorbitan laurate, PPG-5-cetyl alcohol polyether-20, coconut alcohol polyether-7, and PPG-1-PEG-9 lauryl glycol ether. The invention also discloses glabridin liposomes prepared by the self-assembling emulsifier. The self-assembling emulsifier of the invention can self-assemble and emulsify glabridin to form colorless or slightly yellowish clear and transparent glabridin liposomes with uniform particle size and good hydrophilicity; and the preparation process is simple, avoiding the addition of organic solvents such as methanol, ethanol, and chloroform.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a self-assembling emulsifier, glabridin liposome containing the self-assembling emulsifier, and a preparation method and application thereof. Background Art

[0002] Flavonoids are common cosmetic raw materials and are widely used for their daily health benefits such as whitening, anti-oxidation, anti-inflammatory, and anti-aging. However, there are also some potential problems and challenges in practical applications. In particular, flavonoids with a single ingredient and high purity often have poor stability, low solubility, skin irritation, low bioavailability, and compatibility issues.

[0003] Glabridin is a flavonoid extracted from a precious plant called Glycyrrhiza glabra. Glabridin is known as "whitening gold" for its powerful whitening effect. Due to its good biological activity, glabridin also has broad application prospects in the field of cosmetics. However, glabridin also faces many bottlenecks in product application: glabridin is neither soluble in water nor in oil, and it is difficult to stably add it to cosmetics of various dosage forms, especially greatly limiting its flexibility in application in aqueous systems (such as toners and gels). Glycyrrhizin is sensitive to light, heat and oxygen, which may cause it to deteriorate and become ineffective during the production, storage and use of cosmetics, affecting the quality and efficacy of the product; glabridin is difficult to extract and has high purity requirements, resulting in relatively expensive raw material prices. Due to its poor transdermal absorption, it needs to reach a certain amount of addition to exert its efficacy, which increases the formulation cost and is not conducive to its widespread application in low-end and mid-range products.

[0004] In the existing technology, methods such as adding solubilizers, antioxidants, and penetration enhancers are usually used to solve the many problems of glabridin application. For example, glabridin can be dissolved in a polyethylene glycol solubilizer, or antioxidants such as vitamin E can be added to prevent the oxidative degradation of glabridin. In addition, in order to improve the transdermal absorption rate of glabridin, some transdermal enhancers such as azone can also be used. However, the existing technology still has some problems and limitations in solving the problem of glabridin in the application of cosmetic formulas. First, the use of solubilizers may cause the product to increase in stickiness, and the foaming problem is obvious, which affects the use and skin feel of the product. Secondly, the glabridin added product after solubilization is still prone to precipitation, discoloration, and the problem that the aqueous product cannot be clear and transparent. Although the use of antioxidants can prevent the oxidative degradation of glabridin, it may affect the solubility, color and stability of the product. In addition, although the existing transdermal enhancers can improve the transdermal absorption rate of glabridin to a certain extent, they may cause skin irritation, and the effect of improving its transdermal absorption rate is also limited. At the same time, if the formula wants to solve related problems, it may result in a formula with many ingredients and complex factors affecting each other, making it more difficult to control product quality. Therefore, how to find a glabridin composite raw material that can improve the solubility and stability of glabridin, increase its transdermal absorption rate, and reduce the cost of use is the main problem facing current technology.

[0005] Liposome is a small vesicle formed by one or more lipid bilayers, which can be used to encapsulate various drugs or active ingredients. Its excellent biocompatibility and drug loading capacity make it have great potential in the delivery and skin penetration of active ingredients. In addition, liposomes can also improve the solubility and stability of active ingredients and reduce the irritation of active ingredients. However, the preparation of liposomes often has problems such as complex preparation process, low drug loading, uneven particle size distribution, poor stability, high production cost, and residual organic solvents. Therefore, preparing a glabridin liposome with high drug loading, simple preparation process, uniform particle size distribution and high stability can further enhance the raw material advantage of glabridin. Summary of the invention

[0006] The technical problem to be solved in the first aspect of the present invention is to provide a self-assembling emulsifier, which can self-assemble and emulsify glabridin to form glabridin liposomes with uniform particle size and good hydrophilicity.

[0007] The technical problem to be solved in the second aspect of the present invention is to provide a glabridin liposome containing the above-mentioned self-assembling emulsifier, the particle size distribution of the liposome is uniform, and the stability is high; the preparation process is simple, which greatly reduces the preparation cost of the liposome and is conducive to large-scale production; and the addition of organic solvents such as methanol, ethanol, and chloroform is avoided in the preparation process, which is safer, greener and more environmentally friendly.

[0008] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0009] The first aspect of the present invention provides a self-assembling emulsifier, comprising emulsifier A, emulsifier B and emulsifier C; in the self-assembling emulsifier, the mass ratio of emulsifier A, emulsifier B and emulsifier C is (0.05-3):(0.05-1):(0.05-3);

[0010] Among them, the emulsifier A includes at least one of PPG-24-glycerol polyether-24, PPG-decyltetradecyl alcohol polyether-24, and di(PPG-2 myristyl alcohol polyether-10) adipate; the emulsifier B includes at least one of polyquaternium-52, polyquaternium-61, polyquaternium-74, and polyquaternium-37; the emulsifier C includes at least one of PEG-40 hydrogenated castor oil, PEG-80 anhydrous sorbitan laurate, PPG-5-cetoeth-20, coconut alcohol polyether-7, and PPG-1-PEG-9 lauryl glycol ether.

[0011] The inventors found that when emulsifier A, emulsifier B and emulsifier C are used in combination, they have a good emulsifying effect on glabridin. Among them, emulsifier A and emulsifier B can self-assemble and emulsify glabridin through the action of non-covalent bonds such as hydrogen bonds and π-π bonds, forming a stable lipid bilayer structure, encapsulating glabridin therein, thereby forming a vesicle structure with a particle size of less than 150nm; combined with the auxiliary solubilization effect of emulsifier C, glabridin liposomes with more uniform particle size and better hydrophilicity can be obtained.

[0012] In some preferred embodiments of the present invention, the self-assembling emulsifier consists of emulsifier A, emulsifier B and emulsifier C.

[0013] In some preferred embodiments of the present invention, the emulsifier A, emulsifier B and emulsifier C are selected from one of the following combinations:

[0014] a. The emulsifier A is PPG-decyltetradecyl alcohol polyether-24, the emulsifier B is polyquaternium-61, and the emulsifier C is PPG-5-cetyl alcohol polyether-20;

[0015] b. The emulsifier A is PPG-24-glycerol polyether-24, the emulsifier B is polyquaternium-52, and the emulsifier C is PEG-40 hydrogenated castor oil;

[0016] c. The emulsifier A is di(PPG-2 myristyl alcohol polyether-10) adipate, the emulsifier B is polyquaternium-74, and the emulsifier C is PEG-80 sorbitan laurate;

[0017] d. The emulsifier A is PPG-decyltetradecyl alcohol polyether-24, the emulsifier B is polyquaternium-37, and the emulsifier C is cocoether-7;

[0018] e. The emulsifier A is PPG-decyltetradecyl alcohol polyether-24, the emulsifier B is polyquaternium-52, and the emulsifier C is PPG-1-PEG-9 lauryl glycol ether.

[0019] In some preferred embodiments of the present invention, in the self-assembling emulsifier, the mass ratio of emulsifier A, emulsifier B and emulsifier C is (0.1-2):(0.05-0.5):(0.1-2).

[0020] The second aspect of the present invention provides a glabridin liposome, comprising the following components in percentage by mass: 0.005-5% glabridin, 20-60% dispersant, 0.2-5% self-assembling emulsifier, 0.5-3% water-soluble emulsifier, 0.2-5% preservative, 0-1% buffer system, and the balance water; wherein the self-assembling emulsifier is the above-mentioned self-assembling emulsifier.

[0021] In the glabridin liposome of the present invention, the content of glabridin is 0.005-5%, for example, it can be 0.005%, 0.01%, 0.03%, 0.05%, 0.08%, 0.1%, 0.3%, 0.5%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc. Preferably, the content of glabridin is 0.05-5%, more preferably 0.5-2%.

[0022] In the glabridin liposome of the present invention, the content of the self-assembling emulsifier is 0.2-5%, for example, it can be 0.2%, 0.5%, 0.8%, 1.0%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5% and the like.

[0023] Preferably, in the glabridin liposome, the self-assembling emulsifier comprises: emulsifier A 0.05-3%, emulsifier B 0.05-1%, and emulsifier C 0.05-3%. Among them, the content of emulsifier A is 0.05-3%, for example, it can be 0.05%, 0.1%, 0.3%, 0.5%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3% and the like, and is preferably 0.1-2%; the content of emulsifier B is 0.05-1%, for example, it can be 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.8%, 1% and the like, and is preferably 0.05-0.5%; the content of emulsifier C is 0.05-3%, for example, it can be 0.05%, 0.1%, 0.3%, 0.5%, 0.8%, 1%, 1.5%, 2%, 2.5%, 3% and the like, and is preferably 0.1-2%.

[0024] In the glabridin liposome of the present invention, the function of the dispersant is to evenly disperse the glabridin in the alcohol solution so that the next step of encapsulation can be performed. The dispersant can be various polyol solvents that can solubilize and disperse the glabridin, including but not limited to one or more of glycerol, butylene glycol, propylene glycol, and isoprene glycol. Among them, polyols such as glycerol, propylene glycol, and butylene glycol can well form an alcohol dispersion of glabridin; and isoprene glycol, in addition to the function of dispersing and solubilizing, can also promote penetration and assist in antisepsis.

[0025] Further preferably, in the glabridin liposome, the dispersant includes: 10-35% glycerol, 0.5-5% butylene glycol, and 8-25% isoprene glycol. The content of glycerol is 10-35%, for example, 10%, 12%, 14%, 15%, 16%, 18%, 20%, 22%, 24%, 25%, 28%, 30%, 32%, 35%, etc.; the content of butylene glycol is 0.5-5%, for example, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc.; the content of isoprene glycol is 8-25%, for example, 8%, 10%, 12%, 14%, 15%, 16%, 18%, 20%, 22%, 24%, 25%, etc.

[0026] In the glabridin liposome of the present invention, the water-soluble emulsifier is used to make the vesicle structure encapsulating glabridin uniformly dispersed in the aqueous solution to form a uniform dispersion of encapsulated nanoparticles, thereby improving the stability of the liposome preparation. The water-soluble emulsifier includes, but is not limited to, one or more of polysorbate-80, sorbitan polyether-30 tetraoleate, polysorbate-60, and PEG-160 sorbitan triisostearate.

[0027] Preferably, the water-soluble emulsifier is polysorbate 80. Further preferably, the content of polysorbate 80 in the glabridin liposome is 0.5-3%, for example, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, etc. In a preferred embodiment of the present invention, the content of polysorbate 80 in the glabridin liposome is 2%.

[0028] In the glabridin liposomes of the present invention, the preservative can be various commonly used preservative ingredients in cosmetics, including but not limited to one or more of 1,2-hexanediol, 1,2-pentanediol, octanediol, and phenoxyethanol. Preferably, the preservative is a composite preservative composed of 1,2-hexanediol and 1,2-pentanediol.

[0029] Further preferably, in the glabridin liposome, the preservative includes: 0.5-2% 1,2-hexanediol and 0.5-2% 1,2-pentanediol. The content of 1,2-hexanediol is 0.5-2%, for example, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, etc. The content of 1,2-pentanediol is 0.5-2%, for example, it can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, etc.

[0030] In a preferred embodiment of the present invention, the preservatives in the glabridin liposomes include: 1%, 1,2-hexanediol 1% and 1%, 2-pentanediol 1%.

[0031] In some preferred embodiments of the present invention, the glabridin liposomes further include a buffer system. The buffer system can stabilize the pH range of the system and improve the stability of the liposomes. The buffer system includes but is not limited to one or more of lactic acid, sodium lactate, disodium hydrogen phosphate, and potassium dihydrogen phosphate.

[0032] In the glabridin liposome of the present invention, the content of the buffer system is 0-1%, for example, it can be 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0% and the like.

[0033] The third aspect of the present invention provides a method for preparing the glabridin liposome, comprising the following steps:

[0034] S1. Pre-disperse the glabridin powder with a dispersant, add the self-assembly emulsifier, heat to 50-65°C, and stir until completely dissolved;

[0035] S2. Add a water-soluble emulsifier to water, stir evenly, and heat to 50 to 65 ° C; then add the material of step S1 at a speed of 200 to 1000 rpm and stir evenly;

[0036] S3. Cooling to 40-50° C., adding preservatives and buffer system, and stirring evenly to obtain the glabridin liposome.

[0037] Furthermore, the median particle size D50 of the glabridin liposome is 50-150 nm, and the polydispersity index PDI is less than 0.3.

[0038] The fourth aspect of the present invention provides the use of the glabridin liposome in cosmetics.

[0039] Furthermore, the cosmetics include but are not limited to lotions, essences, gels, creams, and skin care products.

[0040] Compared with the prior art, the present invention has the following beneficial effects:

[0041] 1. The self-assembling emulsifier provided by the present invention has a good emulsifying effect on glabridin. The emulsifier A and the emulsifier B can be used together to form a stable lipid bilayer structure, encapsulating glabridin therein to form a vesicle structure with a particle size of less than 150nm; and through the auxiliary solubilization effect of the emulsifier C, glabridin liposomes with more uniform particle size and better hydrophilicity can be obtained.

[0042] 2. The glabridin liposomes provided by the present invention have good stability. The ingredients in the formula have no precipitation and no discoloration in the accelerated stability test, and the performance is stable.

[0043] 3. The glabridin liposomes provided by the present invention significantly improve the solubility of glabridin, so that it can be directly added to dosage forms such as water, milk, cream, etc. without solubilization, and can also remain clear and transparent when used in aqueous preparations.

[0044] 4. The glabridin liposomes provided by the present invention improve the transdermal absorption rate of glabridin, solve the problem of low transdermal absorption rate, and thus greatly improve the efficacy of glabridin.

[0045] 5. The glabridin liposomes provided by the present invention have a simple preparation process, which greatly reduces the cost of liposome preparation and is conducive to large-scale production and transformation; and the addition of organic solvents such as methanol, ethanol, and chloroform is avoided in the preparation process, which is safer, greener and more environmentally friendly. DETAILED DESCRIPTION

[0046] The present invention is further described below in conjunction with specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0047] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0048] Unless otherwise specified, the experimental methods used in the following examples and comparative examples are all conventional methods, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0049] Example 1

[0050] This embodiment provides a glabridin liposome, and its formula is shown in Table 1.

[0051] Table 1

[0052]

[0053]

[0054] The preparation method of the water-soluble glabridin liposomes described in this embodiment is as follows:

[0055] (1) pre-dispersing glabridin powder with glycerol, butylene glycol, and isopentyl glycol, and then adding PPG-decyltetradecyl alcohol polyether-24, polyquaternium-61, and PPG-5-cetyl alcohol polyether-20;

[0056] (2) Heat phase A to 50-60°C and stir for 20 min;

[0057] (3) After the phase A powder is dissolved, heat the phase B to 50-60°C and add the phase A solution to the phase B while stirring at 300 rpm for 10 min.

[0058] (4) When the temperature drops to 45°C, add phase C material to obtain the product.

[0059] Example 2

[0060] This embodiment provides a glabridin liposome, and its formula is shown in Table 2.

[0061] Table 2

[0062]

[0063]

[0064] The preparation method of glabridin liposomes in this example is the same as that in Example 1.

[0065] Example 3

[0066] This embodiment provides a glabridin liposome, and its formula is shown in Table 3.

[0067] Table 3

[0068]

[0069] The preparation method of glabridin liposomes in this example is the same as that in Example 1.

[0070] Example 4

[0071] This embodiment provides a glabridin liposome, and its formula is shown in Table 4.

[0072] Table 4

[0073]

[0074]

[0075] The preparation method of glabridin liposomes in this example is the same as that in Example 1.

[0076] Example 5

[0077] This embodiment provides a glabridin liposome, and its formula is shown in Table 5.

[0078] Table 5

[0079]

[0080] The preparation method of glabridin liposomes in this example is the same as that in Example 1.

[0081] Example 6

[0082] This embodiment provides a glabridin liposome, and its formula is shown in Table 6.

[0083] Table 6

[0084]

[0085]

[0086] The preparation method of glabridin liposomes in this example is the same as that in Example 1.

[0087] Example 7

[0088] This embodiment provides a glabridin liposome, and its formula is shown in Table 7.

[0089] Table 7

[0090]

[0091] The preparation method of glabridin liposomes in this example is the same as that in Example 1.

[0092] Example 8

[0093] This embodiment provides a glabridin liposome, and its formula is shown in Table 8.

[0094] Table 8

[0095]

[0096] The preparation method of glabridin liposomes in this example is the same as that in Example 1.

[0097] Example 9

[0098] This embodiment provides a glabridin liposome, and its formula is shown in Table 9.

[0099] Table 9

[0100]

[0101]

[0102] The preparation method of glabridin liposomes in this example is the same as that in Example 1.

[0103] Comparative Example 1

[0104] This comparative example provides a glabridin liposome, and its formula is shown in Table 10.

[0105] Table 10

[0106]

[0107] The preparation method of the glabridin liposomes in this comparative example is the same as that in Example 1.

[0108] Comparative Example 2

[0109] This comparative example provides a glabridin liposome, and its formula is shown in Table 11.

[0110] Table 11

[0111]

[0112]

[0113] The preparation method of the glabridin liposomes in this comparative example is the same as that in Example 1.

[0114] Comparative Example 3

[0115] This comparative example provides a glabridin liposome, the formula of which is shown in Table 12.

[0116] Table 12

[0117]

[0118] The preparation method of the glabridin liposomes in this comparative example is the same as that in Example 1.

[0119] Comparative Example 4

[0120] This comparative example provides a glabridin liposome, the formula of which is shown in Table 13.

[0121] Table 13

[0122]

[0123]

[0124] The preparation method of the glabridin liposomes in this comparative example is the same as that in Example 1.

[0125] Comparative Example 5

[0126] This comparative example provides a glabridin liposome, the formula of which is shown in Table 14.

[0127] Table 14

[0128]

[0129] The preparation method of the glabridin liposomes in this comparative example is the same as that in Example 1.

[0130] Performance Testing

[0131] 1. Stability test

[0132] The glabridin liposomes prepared in Examples 1 to 8 and Comparative Examples 1 to 5 were placed at 45°C, 20°C, 4°C and 45°C / 4°C cycle (placed at 45°C for 1 day, taken out, and then placed at 4°C for 1 day, and so on) for 1 month, and the color of each sample was observed to see if it changed. The results are shown in Table 15.

[0133] Table 15

[0134]

[0135]

[0136]

[0137] From the results in the above table, it can be seen that the glabridin liposomes prepared in Examples 1 to 9 remain clear and transparent under the test conditions of 45°C, 20°C, 4°C and 45°C / 4°C cycle, and the color does not change significantly to yellow. This shows that the glabridin liposomes prepared in Examples 1 to 9 have good stability.

[0138] Comparative Example 1 uses a traditional solubilization system, and the self-assembling emulsifier of the present invention is not added to its formula. From the results, the stability of the solubilization system is poor, and flocculent precipitation and yellowing of the color all occur under the test conditions of 45°C, 20°C, 4°C and 45°C / 4°C cycle.

[0139] In comparative examples 2 to 4, only two of emulsifier A, emulsifier B and emulsifier C were used in the preparation of glabridin liposomes. From the results, the stability of glabridin liposomes was significantly inferior to that of Example 1, and flocculent precipitation and yellowing occurred. This shows that when preparing glabridin liposomes, only when emulsifiers A, emulsifiers B and emulsifiers C are used in combination can the stability of glabridin liposomes be improved.

[0140] In comparative example 5, when preparing glabridin liposomes, the mass ratio of emulsifier A, emulsifier B and emulsifier C used was 0.005:0.2:1, which was not within the range of (0.05-3):(0.05-1):(0.05-3). From the results, under the test conditions of 45°C, 4°C and 45°C / 4°C cycle, flocculent precipitation occurred. This shows that emulsifier A, emulsifier B and emulsifier C can improve the stability of glycyrrhizin liposomes only when they are used in combination in a specific ratio.

[0141] 2. The median particle size D50 of the glabridin liposomes prepared in Examples 1 to 9 and Comparative Examples 1 to 5 was tested by dynamic light scattering. The results are shown in Table 16.

[0142] Table 16

[0143]

[0144]

[0145] Please refer to Table 16. The median particle size D50 of the glabridin liposomes prepared in Examples 1 to 9 is relatively small, ranging from 50 to 150 nm, and the particle size distribution is relatively uniform, with a PDI index ranging from 0.1 to 0.3.

[0146] Comparative Example 1 uses a traditional solubilization system, and the self-assembling emulsifier of the present invention is not added to its formula. From the results, the median particle size D50 of the liposome has increased significantly compared with the examples, reaching 209.07 nm; at the same time, the PDI index reaches 0.376, which is also significantly higher than Examples 1 to 9, indicating that its particle size distribution is uneven.

[0147] In comparative examples 2 to 4, only two of emulsifier A, emulsifier B, and emulsifier C were used to prepare glabridin liposomes. From the results, the median particle size D50 of the liposomes also increased significantly compared with the examples, and the median particle size D50 exceeded 170 nm, with the highest reaching 254.37 nm; at the same time, the uniformity of the particle size distribution of the liposomes was significantly reduced, and the PDI index exceeded 0.3. This shows that emulsifier A, emulsifier B, and emulsifier C are only used in combination in a specific ratio, which is conducive to reducing the particle size of glabridin liposomes and improving the uniformity of particle size distribution.

[0148] In comparative example 5, when preparing glabridin liposomes, the mass ratio of emulsifier A, emulsifier B and emulsifier C used was 0.005:0.2:1, which was not within the range of (0.05-3):(0.05-1):(0.05-3). From the results, the median particle size D50 of the liposomes increased to 165.23 nm compared with the embodiment; the uniformity of the particle size distribution was significantly reduced, and the PDI index reached 0.314. This shows that only when emulsifier A, emulsifier B and emulsifier C are used in combination in a specific ratio, can the particle size of the liposomes be significantly reduced and the uniformity of the particle size distribution be improved.

[0149] In summary, the present invention provides a glabridin liposome, which uses a self-assembly emulsifier formed by compounding emulsifier A, emulsifier B and emulsifier C in a certain ratio to self-assemble and emulsify glabridin, so as to form a glabridin liposome with uniform particle size, good hydrophilicity and high stability. The preparation process of the liposome is simple, which greatly reduces the preparation cost of the liposome and is conducive to large-scale production and conversion; and the addition of organic solvents such as methanol, ethanol and chloroform is avoided in the preparation process, which is safer, greener and more environmentally friendly.

[0150] The above-described embodiments are only preferred embodiments for fully illustrating the present invention, and the protection scope of the present invention is not limited thereto. Equivalent substitutions or changes made by those skilled in the art based on the present invention are within the protection scope of the present invention. The protection scope of the present invention shall be subject to the claims.

Claims

1. A self-assembling emulsifier, characterized in that It includes emulsifier A, emulsifier B and emulsifier C; in the self-assembling emulsifier, the mass ratio of emulsifier A, emulsifier B and emulsifier C is (0.05-3):(0.05-1):(0.05-3); The emulsifier A includes at least one of PPG-24-glyceryl polyether-24, PPG-decyl tetradecyl alcohol polyether-24, and di(PPG-2 myristyl alcohol polyether-10) adipate; The emulsifier B includes at least one of polyquaternium-52, polyquaternium-61, polyquaternium-74, and polyquaternium-37; The emulsifier C includes at least one of PEG-40 hydrogenated castor oil, PEG-80 sorbitan laurate, PPG-5-cetoeth-20, coceth-7, and PPG-1-PEG-9 lauryl glycol ether.

2. A self-assembling emulsifier according to claim 1, characterized in that: The emulsifier A, emulsifier B and emulsifier C are selected from one of the following combinations: a. The emulsifier A is PPG-decyltetradecyl alcohol polyether-24, the emulsifier B is polyquaternium-61, and the emulsifier C is PPG-5-cetyl alcohol polyether-20; b. The emulsifier A is PPG-24-glycerol polyether-24, the emulsifier B is polyquaternium-52, and the emulsifier C is PEG-40 hydrogenated castor oil; c. The emulsifier A is di(PPG-2 myristyl ether-10) adipate, the emulsifier B is polyquaternium-74, and the emulsifier C is PEG-80 sorbitan laurate; d. The emulsifier A is PPG-decyltetradecyl alcohol polyether-24, the emulsifier B is polyquaternium-37, and the emulsifier C is cocoether-7; e. The emulsifier A is PPG-decyltetradecyl alcohol polyether-24, the emulsifier B is polyquaternium-52, and the emulsifier C is PPG-1-PEG-9 lauryl glycol ether.

3. A self-assembling emulsifier according to claim 2, characterized in that, In the self-assembling emulsifier, the mass ratio of emulsifier A, emulsifier B and emulsifier C is (0.1-2):(0.05-0.5):(0.1-2).

4. A glabridin liposome, characterized in that: The invention comprises the following components by mass percentage: 0.005-5% of glabridin, 20-60% of dispersant, 0.2-5% of self-assembly emulsifier, 0.5-3% of water-soluble emulsifier, 0.2-5% of preservative, 0-1% of buffer system and the balance of water; Wherein, the self-assembling emulsifier is the self-assembling emulsifier according to any one of claims 1 to 3.

5. The glabridin liposome according to claim 4, characterized in that: The dispersant comprises at least one of glycerol, butylene glycol, propylene glycol and isoprene glycol; and / or, The water-soluble emulsifier includes at least one of polysorbate-80, sorbitan polyether-30 tetraoleate, polysorbate-60, and PEG-160 sorbitan triisostearate; and / or, The preservative comprises at least one of 1,2-hexanediol, 1,2-pentanediol, octanediol, and phenoxyethanol; and / or, The buffer system includes at least one of lactic acid, sodium lactate, disodium hydrogen phosphate, and potassium dihydrogen phosphate.

6. The glabridin liposome according to claim 5, characterized in that: In the glabridin liposome, the preservatives include: 0.5-2% of 1,2-hexanediol and 0.5-2% of 1,2-pentanediol.

7. The method for preparing glabridin liposomes according to any one of claims 4 to 6, characterized in that: The following steps are involved: S1. Pre-disperse the glabridin powder with a dispersant, add the self-assembly emulsifier, heat to 50-65°C, and stir until completely dissolved; S2. Add a water-soluble emulsifier to water, stir evenly, and heat to 50 to 65 ° C; then add the material of step S1 at a speed of 200 to 1000 rpm and stir evenly; S3. Cooling to 40-50° C., adding preservatives and buffer system, and stirring evenly to obtain the glabridin liposome.

8. The method for preparing glabridin liposomes according to claim 7, characterized in that: The median particle size D50 of the glabridin liposome is 50-150 nm, and the polydispersity index PDI is less than 0.

3.

9. Use of the glabridin liposome according to any one of claims 4 to 6 in cosmetics.

10. The use according to claim 9, characterized in that: The cosmetics include lotions, essences, gels, creams, and cleaning products.

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