A probiotic-containing composition and its application in the preparation of weight loss products

Abstract

The present invention belongs to the technical field of health products, and particularly relates to a composition containing probiotics, which comprises the following components in parts by weight: 5-10 parts of a mixed enzymolysate, 2-3 parts of Lactobacillus acidophilus, 1-2 parts of Bifidobacterium lactis, 1-2 parts of Lactobacillus bulgaricus, 1-2 parts of Lactobacillus brevis, 1-3 parts of Lactobacillus delbrueckii, 1-2 parts of mannitol, 1-2 parts of isomaltooligosaccharide, and 3-7 parts of a mixed fermentate. The composition prepared by the present invention has a good weight loss effect, especially remarkable for the large-base population with a body weight greater than 80 kg.

Description

Technical Field

[0001] The present invention belongs to the technical field of health products, and particularly relates to a probiotic-containing composition and its application in the preparation of weight loss products. Background Art

[0002] The need for weight loss mainly stems from comprehensive considerations in multiple aspects such as health, appearance, and psychology. First of all, from a health perspective, overweight or obesity is a risk factor for various chronic diseases, including cardiovascular diseases, type 2 diabetes, metabolic syndrome, certain cancers, and osteoarticular diseases. Obesity also increases the risk of respiratory problems (such as sleep apnea syndrome) and causes additional stress on joints, affecting the quality of life.

[0003] The significance of weight loss health products lies in providing a convenient option for those who hope to support the weight loss process through auxiliary means. Although a healthy diet and regular exercise are the basis for weight loss, some people may have difficulty achieving the ideal weight loss effect solely through lifestyle changes due to time constraints, metabolic problems, or other factors. At this time, weight loss health products can be used as a supplementary tool to help regulate metabolism, suppress appetite, promote fat burning, or reduce fat absorption. For example, some health products contain natural ingredients (such as green tea extract, L-carnitine, conjugated linoleic acid, etc.), which are considered helpful for accelerating fat metabolism or inhibiting fat storage. In addition, weight loss health products can also ensure that necessary nutrients are not lacking due to diet control by supplementing vitamins and minerals. However, it should be noted that weight loss health products cannot replace a healthy lifestyle. Their effects are usually auxiliary and should be used under professional guidance to ensure safety and effectiveness. Generally speaking, the significance of weight loss health products lies in providing a scientific and reasonable auxiliary means for those who hope to obtain additional support during the weight loss process, helping them more easily achieve their weight loss goals and maintain a long-term healthy weight. Although there are a wide variety of weight loss products on the market currently, the effects of the weight loss products on the market cannot meet the needs of consumers.

[0004] The following are the main reasons why it is difficult for people with a large body mass to lose weight: 1. Decreased metabolic rate: Decline in basal metabolic rate: Long-term obesity can lead to a decrease in the body's basal metabolic rate (BMR), which means that even at rest, the body consumes less energy. As the weight increases, the body gradually adapts to high-calorie intake, resulting in a slow metabolism. This metabolic adaptation makes weight loss more difficult because even if food intake is reduced, the body will reduce energy consumption to maintain the existing weight.

[0005] Therefore, there is an urgent need for a probiotic-containing composition and its application in the preparation of weight loss products. Summary of the Invention

[0006] The object of the present invention is to provide a probiotic-containing composition and its application in the preparation of weight loss products.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A probiotic-containing composition, comprising the following components in parts by weight: 5-10 parts of a mixed enzymatic hydrolysate, 2-3 parts of Lactobacillus acidophilus, 1-2 parts of Bifidobacterium lactis, 1-2 parts of Lactobacillus bulgaricus, 1-2 parts of Lactobacillus brevis, 1-3 parts of Lactobacillus delbrueckii, 1-2 parts of mannitol, 1-2 parts of isomaltooligosaccharide, and 3-7 parts of a mixed fermentation product.

[0009] Furthermore, the preparation method of the mixed enzymatic hydrolysate comprises the following steps:

[0010] (1) Crushing white kidney beans, green tea, and Chinese yam to a particle size less than 100 mesh to obtain an enzymatic hydrolysis raw material;

[0011] (2) Mixing the enzymatic hydrolysis raw material and water at a weight ratio of 1:(8-10) to obtain an enzymatic hydrolysis solution, adding cellulase to the enzymatic hydrolysis solution, and performing enzymatic hydrolysis for 40-50 min; adjusting the pH value to 9-10 with a 0.5-1 mol / L NaOH aqueous solution, adding alkaline protease, and performing enzymatic hydrolysis for 40-50 min; then adjusting the pH value to 8-9 with a 0.5-1 mol / L HCl aqueous solution, adding bromelain, and continuing enzymatic hydrolysis for 30-40 min, and then adjusting the pH value to 5-6 with a 0.5-1 mol / L HCl aqueous solution, adding pectinase, and continuing enzymatic hydrolysis for 60-80 min. After inactivating the enzyme, an enzymatic hydrolysis product is obtained;

[0012] (3) Centrifuging the enzymatic hydrolysis product, taking the supernatant, filtering the supernatant through an ultrafiltration membrane, obtaining an ultrafiltrate, concentrating and spray-drying to obtain a mixed enzymatic hydrolysate.

[0013] Furthermore, white kidney beans, green tea, and Chinese yam are mixed at a weight ratio of (0.3-0.5):1:(1.4-1.7).

[0014] Furthermore, the addition amount of cellulase is 2000-3000 u / mL of the enzymatic hydrolysis solution; the addition amount of alkaline protease is 1000-2000 u / mL of the enzymatic hydrolysis solution; the addition amount of bromelain is 1000-2000 u / mL of the enzymatic hydrolysis solution; the addition amount of pectinase is 1000-2000 u / mL of the enzymatic hydrolysis solution.

[0015] Furthermore, the supernatant is filtered through an ultrafiltration membrane with a molecular weight of 4000-5000 Da.

[0016] Furthermore, the preparation method of the mixed fermentation product comprises the following steps:

[0017] (1) Crush lotus leaf, hawthorn and citrus to a particle size less than 100 mesh to obtain a mixed raw material;

[0018] (2) Place the mixed raw material in a low temperature of -30°C for 2 - 3 minutes first, then rapidly heat it to 23 - 25°C within 2 seconds, and repeat 2 - 3 times; then pressurize it to 20 - 25 MPa, keep it for 3 - 5 minutes, and then release the pressure to atmospheric pressure, and repeat 2 - 3 times to obtain a pretreated mixed raw material;

[0019] (3) Mix the pretreated mixed raw material and water at a weight ratio of 1:(8 - 10) to obtain a mixed liquid, add a compound bacterium to the mixed liquid, and perform anaerobic fermentation for 10 - 15 hours. After sterilization, a fermentation liquid is obtained;

[0020] (4) Centrifuge the fermentation liquid, take the supernatant, filter the supernatant through an ultrafiltration membrane with a molecular weight of 3000 - 5000 Da, obtain an ultrafiltrate, concentrate it, and spray dry it to obtain a mixed fermented product.

[0021] Further, mix lotus leaf, hawthorn and citrus at a weight ratio of 1:(1.2 - 1.5):(0.4 - 0.7).

[0022] Further, the compound bacterium includes Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the addition amount of Lactobacillus casei is 10 7 - 10 8 CFU / mL of the mixed liquid; the addition amount of Lactobacillus brevis is 10 9 - 10 10 CFU / mL of the mixed liquid; the addition amount of Bifidobacterium longum is 10 8 - 10 9 CFU / mL of the mixed liquid.

[0023] Further, the preparation method of the probiotic - containing composition includes the following steps: Weigh the components according to the weight ratio and mix them evenly to obtain it.

[0024] The present invention provides the application of the probiotic - containing composition in the preparation of weight - loss products.

[0025] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0026] 1. In the mixed enzymatic hydrolysate prepared by enzymatic hydrolysis in the present invention, white kidney beans are rich in α-amylase inhibitors, which can inhibit the activity of α-amylase in the small intestine and delay the digestion and absorption of carbohydrates. This helps prevent a rapid increase in blood sugar, reduce insulin secretion, and avoid further exacerbation of insulin resistance. By inhibiting α-amylase, white kidney bean extract can reduce the sharp rise in postprandial blood sugar, stabilize blood sugar levels, and reduce hunger and overeating. Catechins in green tea (such as EGCG) have powerful antioxidant effects, which can activate lipolytic enzymes in fat cells and promote the oxidation and metabolism of fat. Studies have shown that green tea extract can increase energy consumption and reduce fat accumulation, especially in the abdominal area. Polyphenols in green tea can also improve insulin sensitivity, help the body better utilize glucose, and reduce fat storage. In addition, green tea has an anti-inflammatory effect, which can reduce chronic inflammation associated with obesity and further improve metabolic health. Polysaccharides in Chinese yam can improve insulin sensitivity, help the body better utilize glucose, and reduce fat storage. In addition, Chinese yam has an anti-inflammatory effect, which can reduce chronic inflammation associated with obesity and further improve the symptoms of metabolic syndrome.

[0027] 2. In the composition of the present invention, lotus leaf, hawthorn, and citrus are mixed and fermented with specific bacteria, and the resulting product can help overweight people over 80 kg lose weight through various channels. Lotus leaf is rich in alkaloids (such as nuciferine), which can inhibit the absorption of fat in the intestine and reduce the accumulation of body fat. During the fermentation process, specific bacteria can further decompose the complex components in lotus leaf, release more active substances, and enhance its effect of inhibiting fat absorption. Flavonoids in lotus leaf have antioxidant effects, which can activate lipolytic enzymes in fat cells and promote the oxidation and metabolism of fat. The fermentation process can increase the bioavailability of these flavonoids, making them more easily absorbed by the human body, thus accelerating fat burning.

[0028] Hawthorn contains rich organic acids (such as chlorogenic acid and caffeic acid) and lipase, which can promote gastric juice secretion and accelerate the digestion of food, especially the digestion of greasy food. During the fermentation process, specific bacteria can further decompose the organic acids in hawthorn, producing more metabolites and enhancing its effects on promoting digestion and fat metabolism. The flavonoid compounds and triterpenoid compounds in hawthorn have the effect of reducing blood lipid, which can lower the levels of triglycerides and low-density lipoprotein (LDL) cholesterol in the blood and reduce the risk of arteriosclerosis. The fermentation process can improve the biological activity of these compounds and enhance their effects on reducing blood lipid. Oranges contain rich vitamin C and flavonoids (such as hesperidin and naringin), which have strong antioxidant effects, can activate the lipoxidase in fat cells, and promote the oxidation and metabolism of fat. During the fermentation process, specific bacteria can further decompose the flavonoids in oranges, releasing more active substances and enhancing its effect on promoting fat oxidation.

[0029] Specific bacteria produce short-chain fatty acids (SCFAs) during the fermentation process, such as acetic acid, propionic acid, and butyric acid. These short-chain fatty acids can not only serve as an energy source but also regulate the balance of the gut microbiota, promote the growth of beneficial bacteria, and inhibit the reproduction of harmful bacteria. A healthy gut microbiota helps improve metabolic health, reduce inflammatory responses, and promote the decomposition and metabolism of fat. The short-chain fatty acids produced during the fermentation process can also enhance the gut barrier function, reduce the absorption of harmful substances, and prevent the occurrence of gut leakage. This helps improve gut health, reduce chronic inflammation associated with obesity, and further promote fat burning.

[0030] 3. The present invention combines Lactobacillus acidophilus, Bifidobacterium lactis, Lactobacillus bulgaricus, Lactobacillus brevis, and Lactobacillus delbrueckii, which can significantly improve the weight loss effect. This combined microbiota promotes fat metabolism, improves gut health, regulates blood sugar and insulin sensitivity, and enhances satiety and reduces appetite through various synergistic effects in multiple aspects. Lactobacillus acidophilus can produce short-chain fatty acids (SCFAs), especially acetic acid and propionic acid, which can inhibit the fatty acid synthase in fat cells and reduce the synthesis and storage of fat. In addition, Lactobacillus acidophilus can also promote the decomposition of fat by regulating the gut microbiota, reducing the endotoxin produced by harmful bacteria, and reducing chronic inflammation. Bifidobacterium lactis can also promote the decomposition and metabolism of fat by activating the lipase in fat cells, helping the body consume fat more effectively. Lactobacillus bulgaricus can also maintain the balance of the gut microbiota by competitively inhibiting the growth of harmful bacteria, reducing the endotoxin produced by harmful bacteria, and reducing chronic inflammation, thereby promoting the decomposition of fat. Lactobacillus brevis can enhance the gut barrier function by producing mucin and tight junction proteins, reducing the absorption of harmful substances, and preventing the occurrence of gut leakage. This helps improve gut health, reduce chronic inflammation associated with obesity, and further promote fat burning. Detailed implementation mode

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0032] Embodiment 1

[0033] This embodiment provides a probiotic-containing composition, including the following components in parts by weight: 5 parts of mixed enzymolysate, 2 parts of Lactobacillus acidophilus, 1 part of Bifidobacterium lactis, 1 part of Lactobacillus bulgaricus, 1 part of Lactobacillus brevis, 1 part of Lactobacillus delbrueckii, 1 part of mannitol, 1 part of isomaltooligosaccharide, and 3 parts of mixed fermentate.

[0034] The preparation method of the mixed enzymolysate includes the following steps:

[0035] (1) Crush white kidney beans, green tea, and Chinese yam to a particle size less than 100 mesh to obtain an enzymolysis raw material;

[0036] (2) Mix the enzymolysis raw material and water at a weight ratio of 1:8 to obtain an enzymolysis solution. Add cellulase to the enzymolysis solution and enzymolyze for 40 min; adjust the pH value to 9 with 0.5 mol / L NaOH aqueous solution, add alkaline protease, and enzymolyze for 40 min; then adjust the pH value to 8 with 0.5 mol / L HCl aqueous solution, add bromelain, and continue to enzymolyze for 30 min. Then adjust the pH value to 5 with 0.5 mol / L HCl aqueous solution, add pectinase, and continue to enzymolyze for 60 min. After inactivating the enzyme, obtain an enzymolysis product;

[0037] (3) Centrifuge the enzymolysis product, take the supernatant, filter the supernatant through an ultrafiltration membrane, obtain an ultrafiltrate, concentrate and spray dry to obtain a mixed enzymolysate.

[0038] Mix white kidney beans, green tea, and Chinese yam at a weight ratio of 0.3:1:1.4.

[0039] The addition amount of cellulase is 2000 u / mL of enzymolysis solution; the addition amount of alkaline protease is 1000 u / mL of enzymolysis solution; the addition amount of bromelain is 1000 u / mL of enzymolysis solution; the addition amount of pectinase is 1000 u / mL of enzymolysis solution.

[0040] Filter the supernatant through an ultrafiltration membrane with a molecular weight of 4000 Da.

[0041] The preparation method of the mixed fermentate includes the following steps:

[0042] (1) Crush lotus leaf, hawthorn and citrus to a particle size less than 100 mesh to obtain a mixed raw material;

[0043] (2) Place the mixed raw material in a low temperature of -30°C for 2 minutes first, then rapidly heat it to 23°C within 2 seconds, and repeat 2 times; then pressurize to 20 MPa, hold for 3 minutes, and then release the pressure to atmospheric pressure, and repeat 2 times to obtain a pretreated mixed raw material;

[0044] (3) Mix the pretreated mixed raw material and water at a weight ratio of 1:8 to obtain a mixed solution, add a compound bacterium to the mixed solution, and perform anaerobic fermentation for 10 hours. After sterilization, a fermentation broth is obtained;

[0045] (4) Centrifuge the fermentation broth, take the supernatant, filter the supernatant through a ultrafiltration membrane with a molecular weight of 3000 Da, obtain an ultrafiltrate, concentrate and spray dry to obtain a mixed fermented product.

[0046] Mix lotus leaf, hawthorn and citrus at a weight ratio of 1:1.2:0.4.

[0047] The compound bacterium includes Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the addition amount of Lactobacillus casei is 10 7 CFU / mL of the mixed solution; the addition amount of Lactobacillus brevis is 10 9 CFU / mL of the mixed solution; the addition amount of Bifidobacterium longum is 10 8 CFU / mL of the mixed solution.

[0048] The preparation method of the probiotic-containing composition includes the following steps: Weigh the components according to the weight ratio and mix them evenly to obtain it.

[0049] Example 2

[0050] This example provides a probiotic-containing composition, including the following components in parts by weight: 10 parts of mixed enzymolysate, 3 parts of Lactobacillus acidophilus, 2 parts of Bifidobacterium lactis, 2 parts of Lactobacillus bulgaricus, 2 parts of Lactobacillus brevis, 3 parts of Lactobacillus delbrueckii, 2 parts of mannitol, 2 parts of isomaltooligosaccharide, and 7 parts of mixed fermented product.

[0051] The preparation method of the mixed enzymolysate includes the following steps:

[0052] (1) Crush white kidney bean, green tea and Chinese yam to a particle size less than 100 mesh to obtain an enzymolysis raw material;

[0053] (2) Mix the enzymolysis raw material and water at a weight ratio of 1:10 to obtain an enzymolysis solution. Add cellulase to the enzymolysis solution and carry out enzymolysis for 50 min. Adjust the pH value to 10 using 1 mol / L NaOH aqueous solution, add alkaline protease, and carry out enzymolysis for 50 min. Then adjust the pH value to 9 using 1 mol / L HCl aqueous solution, add bromelain, and continue enzymolysis for 40 min. Then adjust the pH value to 6 using 1 mol / L HCl aqueous solution, add pectinase, and continue enzymolysis for 80 min. After inactivating the enzyme, obtain an enzymolysis product;

[0054] (3) Centrifuge the enzymolysis product, take the supernatant, filter the supernatant through an ultrafiltration membrane, obtain an ultrafiltrate, concentrate and spray dry to obtain a mixed enzymolysate.

[0055] Mix white kidney beans, green tea and Chinese yams at a weight ratio of 0.5:1:1.7.

[0056] The addition amount of cellulase is 3000 u / mL of the enzymolysis solution; the addition amount of alkaline protease is 2000 u / mL of the enzymolysis solution; the addition amount of bromelain is 2000 u / mL of the enzymolysis solution; the addition amount of pectinase is 2000 u / mL of the enzymolysis solution.

[0057] Filter the supernatant through an ultrafiltration membrane with a molecular weight of 5000 Da.

[0058] The preparation method of the said mixed ferment includes the following steps:

[0059] (1) Crush lotus leaves, hawthorns and citrus fruits to a particle size less than 100 mesh to obtain a mixed raw material;

[0060] (2) Place the mixed raw material in a low temperature of -30 °C for 3 min first, then rapidly heat up to 25 °C within 2 seconds, and repeat 3 times; then pressurize to 25 MPa, keep for 5 min, and then release the pressure to normal pressure, and repeat 3 times to obtain a pretreated mixed raw material;

[0061] (3) Mix the pretreated mixed raw material and water at a weight ratio of 1:10 to obtain a mixed solution. Add a compound bacterium to the mixed solution and carry out anaerobic fermentation for 15 h. After sterilization, obtain a fermentation broth;

[0062] (4) Centrifuge the fermentation broth, take the supernatant, filter the supernatant through an ultrafiltration membrane with a molecular weight of 5000 Da, obtain an ultrafiltrate, concentrate and spray dry to obtain a mixed ferment.

[0063] Mix lotus leaves, hawthorns and citrus fruits at a weight ratio of 1:1.5:0.7.

[0064] The compound bacterium includes Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the addition amount of Lactobacillus casei is 10 8 CFU / mL of the mixed solution; the addition amount of Lactobacillus brevis is 1010 CFU / mL mixed solution; the addition amount of Bifidobacterium longum is 10 9 CFU / mL mixed solution.

[0065] The preparation method of the probiotic-containing composition comprises the following steps: weighing the components according to the weight ratio, and mixing evenly to obtain the composition.

[0066] Comparative Example 1

[0067] The difference between this comparative example and Example 1 is: a probiotic-containing composition, comprising the following components in parts by weight: 3 parts of mixed hydrolysate, 4 parts of Lactobacillus acidophilus, 3 parts of Bifidobacterium lactis, 3 parts of Lactobacillus delbrueckii, 1 part of mannitol, 1 part of isomaltooligosaccharide, and 1 part of mixed ferment.

[0068] Comparative Example 2

[0069] The difference between this comparative example and Example 1 is: mixing white kidney beans, green tea and Chinese yam in a weight ratio of 1:1:1.

[0070] Comparative Example 3

[0071] The difference between this comparative example and Example 1 is: the addition amount of cellulase: 1000 u / mL enzyme hydrolysis solution; the addition amount of alkaline protease is 3000 u / mL enzyme hydrolysis solution; the addition amount of bromelain is 500 u / mL enzyme hydrolysis solution; the addition amount of pectinase is 500 u / mL enzyme hydrolysis solution.

[0072] Comparative Example 4

[0073] The difference between this comparative example and Example 1 is: mixing lotus leaf, hawthorn and citrus in a weight ratio of 1:1:1.

[0074] Comparative Example 5

[0075] The difference between this comparative example and Example 1 is: the complex bacteria include Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the addition amount of Lactobacillus casei is 10 9 CFU / mL mixed solution; the addition amount of Lactobacillus brevis is 10 8 CFU / mL mixed solution; the addition amount of Bifidobacterium longum is 10 7 CFU / mL mixed solution.

[0076] Performance Test

[0077] 1. Experimental Subjects and Methods

[0078] 1.1 Experimental Subjects

[0079] All the subjects in this experiment were evaluated for body mass index before the experiment and were all obese. Among the 80 subjects, 40 were male and 40 were female; their ages were between 18 and 50, and the average age was (34.7 ± 7.8) years.

[0080] 1.2 Experimental methods

[0081] Grouping: 80 subjects were randomly divided into 8 groups, namely negative control group (N = 10), Example 1 (N = 10), Example 2 (N = 10), Comparative Example 1 (N = 10), Comparative Example 2 (N = 10), Comparative Example 3 (N = 10), Comparative Example 4 (N = 10), and Comparative Example 5 (N = 10). The negative control group took placebo, 2 capsules per day; the examples and comparative examples were continuously taken for 8 weeks according to the above method. During the whole experiment period, the diet was not controlled, no extra exercise was deliberately increased, and other weight loss foods and drugs were prohibited from taking.

[0082] 1.3 Test indicators

[0083] 1.3.1 Body composition test: Before and after the experiment, the body weight, body fat percentage, and body mass index of the subjects were measured weekly using a DX200 super body composition analyzer.

[0084] 1.4 Data statistics

[0085] The obtained data was processed by Excel, and the test data was statistically analyzed using the single-blind method and t-test, and expressed as mean ± SD.

[0086] The results are shown in Table 1.

[0087] Table 1: Changes in the body weight of the subjects before and after the experiment

[0088]

[0089] It can be seen from Table 1 that the composition described in the present invention has excellent weight loss effect.

[0090] By comparing Example 1 with Comparative Example 1, it can be seen that the ratio and composition of the composition described in the present invention can significantly improve the weight loss effect.

[0091] By comparing Example 1 with Comparative Examples 2 - 3, it can be seen that the mixed enzymolysate prepared by the specific enzymolysis method of the present invention significantly improves the weight loss effect.

[0092] By comparing Example 1 with Comparative Examples 4 - 5, it can be seen that the mixed fermentate prepared by the specific fermentation method of the present invention significantly improves the weight loss effect.

[0093] The above is the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle described in the present invention, several improvements and refinements can still be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A composition containing probiotics, characterized in that: The invention comprises the following components in parts by weight: 5-10 parts of mixed enzymatic hydrolysate, 2-3 parts of Lactobacillus acidophilus, 1-2 parts of Bifidobacterium lactis, 1-2 parts of Lactobacillus bulgaricus, 1-2 parts of Lactobacillus breve, 1-3 parts of Lactobacillus delbrueckii, 1-2 parts of mannitol, 1-2 parts of isomaltooligosaccharide, and 3-7 parts of mixed fermentation product; The preparation method of the mixed enzymolyte comprises the following steps: (1) Grinding white kidney beans, green tea and yam to a particle size of less than 100 mesh to obtain enzymatic hydrolysis raw materials; (2) Mixing the enzymatic hydrolysis raw material and water in a weight ratio of 1:(8-10) to obtain an enzymatic hydrolysis solution, adding cellulase to the enzymatic hydrolysis solution, and performing enzymatic hydrolysis for 40-50 minutes; adjusting the pH value to 9-10 using a 0.5-1 mol / L NaOH aqueous solution, adding alkaline protease, and performing enzymatic hydrolysis for 40-50 minutes; then adjusting the pH value to 8-9 using a 0.5-1 mol / L HCl aqueous solution, adding bromelain, and continuing enzymatic hydrolysis for 30-40 minutes; then adjusting the pH value to 5-6 using a 0.5-1 mol / L HCl aqueous solution, adding pectinase, and continuing enzymatic hydrolysis for 60-80 minutes, and obtaining an enzymatic hydrolysis product after inactivating the enzyme; (3) centrifuging the enzymatic hydrolysate, taking the supernatant, filtering the supernatant through an ultrafiltration membrane to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed enzymatic hydrolysate; Mix white kidney beans, green tea and Chinese yam in a weight ratio of (0.3-0.5):1:(1.4-1.7); The amount of cellulase added is 2000-3000u / mL enzymatic solution; the amount of alkaline protease added is 1000-2000u / mL enzymatic solution; the amount of bromelain added is 1000-2000u / mL enzymatic solution; the amount of pectinase added is 1000-2000u / mL enzymatic solution; The method for preparing the mixed fermentation product comprises the following steps: (1) Grinding lotus leaves, hawthorns and citrus fruits to a particle size of less than 100 mesh to obtain a mixed raw material; (2) The mixed raw material is first placed in a low temperature of -30°C for 2-3 minutes, then rapidly heated to 23-25°C within 2 seconds, and repeated 2-3 times; then pressurized to 20-25MPa, maintained for 3-5 minutes, and then depressurized to normal pressure, and repeated 2-3 times to obtain a pretreated mixed raw material; (3) mixing the pretreated mixed raw material and water in a weight ratio of 1:(8-10) to obtain a mixed solution, adding composite bacteria to the mixed solution, performing anaerobically fermentation for 10-15 hours, and sterilizing to obtain a fermentation liquid; (4) centrifuging the fermentation broth, taking the supernatant, filtering the supernatant through an ultrafiltration membrane with a molecular weight of 3000-5000Da to obtain an ultrafiltrate, concentrating and spray-drying to obtain a mixed fermentation product; Mix lotus leaves, hawthorns and citrus in a weight ratio of 1: (1.2-1.5): (0.4-0.7); The composite bacteria include Lactobacillus casei, Lactobacillus brevis and Bifidobacterium longum; the amount of Lactobacillus casei added is 10 7 -10 8 CFU / mL mixed solution; the amount of Lactobacillus brevis added was 10 9 -10 10 CFU / mL mixed solution; the amount of Bifidobacterium longum added was 10 8 -10 9 CFU / mL mixed solution.

2. The composition containing probiotics according to claim 1, characterized in that The supernatant was filtered through an ultrafiltration membrane with a molecular weight of 4000-5000Da.

3. The composition containing probiotics according to claim 1, characterized in that The preparation method of the composition containing probiotics comprises the following steps: weighing components according to weight ratio, and mixing them evenly to obtain the composition.

4. Use of the probiotic composition according to any one of claims 1 to 3 in the preparation of a weight loss product.

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