A composition containing citrus polyphenols and citrus powder for burning fat and losing weight, and its preparation method and application

Abstract

The present invention belongs to the technical field of extracting effective pharmaceutical ingredients, and particularly relates to a composition containing citrus polyphenols and citrus powder for burning fat and losing weight, and a preparation method and application thereof. The composition comprises a spray-dried powder prepared from citrus fruit filtrate, Flammulina velutipes fiber, soy protein isolate, aronia berry extract, and black tea extract, and a compound probiotic, wherein the compound probiotic comprises Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis, and Lactobacillus casei. The present invention adopts compound spraying of Flammulina velutipes fiber, soy protein isolate, aronia berry extract, black tea extract, and citrus fruit filtrate to improve the stability of total polyphenols and Vc in citrus fruits. The obtained citrus powder is further mixed with a compound probiotic to prepare a composition with pharmacologically active ingredients, which can effectively burn fat and lose weight and can be applied to weight loss drugs.

Description

Technical Field

[0001] The invention belongs to the technical field of extracting effective ingredients of medicines, and its international classification number is A61K36. It particularly relates to a composition containing citrus polyphenols and citrus powder for burning fat and losing weight, and a preparation method and application thereof. Background Art

[0002] In recent years, with the continuous improvement of living standards, people pay more and more attention to their own health, and taking some medicines appropriately to maintain health has gradually become a trend in daily consumption.

[0003] Due to the obesity faced by modern people due to changes in their lifestyle and eating habits and the overall health problems caused by obesity, as well as the increased demand for food by modern people, it is of great significance to study a composition for burning fat and losing weight that can effectively reduce body fat content and slow down the chance of regaining weight, as well as its preparation method and application. Summary of the invention

[0004] In view of this, the present invention provides a composition containing citrus polyphenols and citrus powder for burning fat and losing weight, and a preparation method and application thereof.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] A composition containing citrus polyphenols and citrus powder for burning fat and losing weight comprises citrus powder and probiotics.

[0007] The citrus powder comprises citrus fruit filtrate, enoki mushroom fiber, soy protein isolate, aronia berry extract and black tea extract.

[0008] Preferably, the citrus powder comprises 80-90 parts of citrus fruit filtrate, 1-2 parts of Flammulina velutipes fiber, 1-5 parts of soy protein isolate, 5-8 parts of aronia berry extract, and 2-5 parts of black tea extract in parts by mass.

[0009] The citrus fruit filtrate is prepared by taking citrus fruits with skin as raw materials.

[0010] The dietary fiber content of the citrus powder is ≥40%, the total polyphenol content is ≥5%, and the VC content is ≥2%.

[0011] The caffeine content of the black tea extract is ≥0.5%.

[0012] The probiotics are composite probiotics, and the composite probiotics include Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis, and Lactobacillus casei.

[0013] Further preferably, the content of the composite probiotics is 1-5*10^ 8 cfu / g.

[0014] Preferably, the citrus powder composition includes 90 parts by mass of citrus powder and 1 - 10 parts by mass of probiotics.

[0015] The preparation method of the composition containing citrus polyphenols and citrus powder for burning fat and losing weight includes the following steps:

[0016] S1. Break and homogenize the citrus fruits with peel, then filter to obtain the citrus fruit filtrate;

[0017] S2. Add fresh Flammulina velutipes to 5 - 10 times its volume of water, extract at 80 - 90 °C, filter, wash, separate, dry, obtain the crude fiber of Flammulina velutipes, pulverize it, and sieve it through a 20 - 40 mesh sieve to obtain Flammulina velutipes fiber;

[0018] S3. Pulverize the dried Aronia melanocarpa, sieve it through a 100 - 200 mesh sieve, add 30 - 50 times its volume of water, extract at 70 - 90 °C for 0.5 - 1 h, filter and take the filtrate, concentrate it to 1 / 3 - 1 / 5 of its original volume to obtain the Aronia melanocarpa extract;

[0019] S4. Pulverize the dried black tea, sieve it through a 100 - 200 mesh sieve, add 30 - 50 times its volume of water, extract at 80 - 100 °C for 0.5 - 1 h, filter and take the filtrate, concentrate it to obtain the black tea extract;

[0020] S5. Mix the citrus fruit filtrate, Flammulina velutipes fiber, soy protein isolate, Aronia melanocarpa extract, and black tea extract, and perform spray drying to obtain citrus powder.

[0021] S6. Mix the probiotics and the citrus powder to obtain the citrus powder composition.

[0022] The composition containing citrus polyphenols and citrus powder for burning fat and losing weight can be used to prepare drugs for burning fat and losing weight.

[0023] Beneficial effects

[0024] 1. The present invention uses the compounding of Flammulina velutipes fiber, soy protein isolate, Aronia melanocarpa extract, and black tea extract with the citrus fruit filtrate for spray drying, which can effectively extract the medicinal components, and is beneficial to maintaining the activity of Vc, thereby increasing the Vc content in the citrus powder and enhancing the effect of resisting human aging.

[0025] 2. The present invention uses the compounding of citrus powder and compound probiotics to achieve a synergistic effect of burning fat and losing weight, and the preparation process is simple, and it can be used to prepare drugs. Specific embodiments

[0026] The technical solution of the present invention will be further described below in conjunction with specific embodiments. The compound probiotics used in the following examples were purchased from Shandong Pingo Pharmaceutical Co., Ltd. The compound probiotics include Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis, and Lactobacillus casei. Other raw materials, unless otherwise specified, can be obtained from conventional commercial channels; the processes used, unless otherwise specified, are conventional processes in the art.

[0027] Example 1

[0028] A composition containing citrus polyphenols and citrus powder for burning fat and losing weight, calculated by mass fraction, includes 90 parts by mass of citrus powder and 5 parts by mass of compound probiotics (1*10^8 cfu / g).

[0029] The preparation method of the citrus powder composition is as follows:

[0030] S1. Break and homogenize the citrus fruits with peel, filter to obtain citrus fruit filtrate;

[0031] S2. Add fresh Flammulina velutipes to 10 times its volume of water, stir and extract at 80°C for 1 h at 200 rpm, filter through a 400-mesh sieve, wash with 10 times its volume of water, filter through a 400-mesh sieve to obtain solids, dry at 45°C to obtain Flammulina velutipes crude fiber, pulverize, and sieve through a 40-mesh sieve to obtain Flammulina velutipes fiber (water content is 6.5 wt%);

[0032] S3. Pulverize dried Aronia melanocarpa, sieve through a 100-mesh sieve, add 30 times its volume of water, stir and extract at 90°C for 0.5 h at 400 rpm, filter and concentrate the filtrate to 1 / 5 of its volume to obtain Aronia melanocarpa extract;

[0033] S4. Pulverize dried black tea, sieve through a 100-mesh sieve, add 50 times its volume of water, stir and extract at 100°C for 0.5 h at 400 rpm, filter and concentrate the filtrate to 1 / 20 of its volume to obtain black tea extract;

[0034] S5. According to the mass fraction, mix and stir 90 parts of citrus fruit filtrate, 1 part of Flammulina velutipes fiber, 1 part of soy protein isolate, 5 parts of Aronia melanocarpa extract, and 3 parts of black tea extract, and perform spray drying. The inlet temperature of spray drying is 220°C, and the outlet temperature is 110°C to obtain citrus powder.

[0035] S6. Mix 5 parts by mass of probiotics and 90 parts by mass of citrus powder to obtain a citrus powder composition (water content is 5.7 wt%).

[0036] The composition containing citrus polyphenols and citrus powder is made into tablets, adding 5 parts by mass of carboxymethyl cellulose, granulating, and tableting to make tablets with a mass of 0.2 g per tablet of the citrus powder composition.

[0037] Example 2

[0038] A composition containing citrus polyphenols and citrus powder for burning fat and losing weight, by mass, includes 90 parts by mass of citrus powder and 1 part by mass of compound probiotics (1*10^8 cfu / g).

[0039] The preparation method of the citrus powder composition is as follows:

[0040] S1. Break and homogenize the citrus fruits with peel, and filter to obtain citrus fruit filtrate;

[0041] S2. Add fresh Flammulina velutipes to 5 times its volume of water, stir and extract at 90 °C for 1 h at 200 rpm, filter through 400 mesh, wash with 10 times its volume of water, filter through 400 mesh to obtain solids, dry at 45 °C to obtain Flammulina velutipes crude fiber, crush, and sieve through 20 mesh to obtain Flammulina velutipes fiber (water content is 6.6 wt%);

[0042] S3. Crush the dried Aronia melanocarpa, sieve through 200 mesh, add 50 times its volume of water, stir and extract at 80 °C for 1 h at 500 rpm, filter and concentrate the filtrate to 1 / 3 of its volume to obtain Aronia melanocarpa extract;

[0043] S4. Crush the dried black tea, sieve through 200 mesh, add 30 times its volume of water, extract at 80 °C for 1 h at 300 rpm, filter and concentrate the filtrate to 1 / 15 of its volume to obtain black tea extract;

[0044] S5. By mass, mix and stir 80 parts of citrus fruit filtrate, 2 parts of Flammulina velutipes fiber, 5 parts of soy protein isolate, 8 parts of Aronia melanocarpa extract, and 5 parts of black tea extract, and perform spray drying. The inlet temperature of spray drying is 180 °C, and the outlet temperature is 105 °C to obtain citrus powder (water content is 5.7 wt%).

[0045] S6. Mix 1 part by mass of probiotics and 90 parts by mass of citrus powder to obtain the citrus powder composition.

[0046] Apply the citrus powder composition to make tablets, add 9 parts by mass of carboxymethyl cellulose, granulate, and press into tablets with a mass of 0.2 g per tablet containing the citrus powder composition.

[0047] Example 3

[0048] A composition containing citrus polyphenols and citrus powder for burning fat and losing weight, by mass, includes 90 parts by mass of citrus powder and 10 parts by mass of compound probiotics (1*10^8 cfu / g).

[0049] The preparation method of the citrus powder composition is as follows:

[0050] S1. Break and homogenize the citrus fruits with peel, then filter to obtain the citrus fruit filtrate;

[0051] S2. Add fresh Flammulina velutipes to 5 times its volume of water, stir and extract at 90 °C at 100 rpm for 1 h, filter through a 400-mesh sieve, wash with 10 times its volume of water, filter through a 400-mesh sieve to obtain the solid, dry at 60 °C to obtain the crude Flammulina velutipes fiber, pulverize, and sieve through a 20-mesh sieve to obtain Flammulina velutipes fiber (with a water content of 5.5 wt%);

[0052] S3. Pulverize the dried Aronia melanocarpa, sieve through a 200-mesh sieve, add 50 times its volume of water, stir and extract at 80 °C at 300 rpm for 1 h, filter and concentrate the filtrate to 1 / 3 of its volume to obtain the Aronia melanocarpa extract;

[0053] S4. Pulverize the dried black tea, sieve through a 200-mesh sieve, add 30 times its volume of water, extract at 80 °C at 200 rpm for 1 h, filter and concentrate the filtrate to 1 / 10 of its volume to obtain the black tea extract;

[0054] S5. Mix and stir 85 parts by mass of the citrus fruit filtrate, 3 parts by mass of Flammulina velutipes fiber, 3 parts by mass of soy protein isolate, 6 parts by mass of Aronia melanocarpa extract, and 3 parts by mass of black tea extract, and perform spray drying. The inlet temperature of spray drying is 220 °C, and the outlet temperature is 110 °C to obtain citrus powder (with a water content of 5.7 wt%);

[0055] S6. Mix 10 parts by mass of probiotics and 90 parts by mass of citrus powder to obtain the citrus powder composition.

[0056] Apply the citrus powder composition to make tablets. After granulation and tableting, tablets with a mass of 0.2 g per tablet containing the citrus powder composition are made.

[0057] Comparative Example 1

[0058] A composition containing citrus polyphenols and citrus powder for burning fat and losing weight, including 90 parts by mass of citrus powder according to mass parts.

[0059] The preparation method of the citrus powder composition is as follows:

[0060] S1. Break and homogenize the citrus fruits with peel, then filter to obtain the citrus fruit filtrate;

[0061] S2. Add fresh Flammulina velutipes to 10 times its volume of water, stir and extract at 80 °C at 200 rpm for 1 h, filter through a 400-mesh sieve, wash with 10 times its volume of water, filter through a 400-mesh sieve to obtain the solid, dry at 45 °C to obtain the crude Flammulina velutipes fiber, pulverize, and sieve through a 40-mesh sieve to obtain Flammulina velutipes fiber (with a water content of 6.6 wt%);

[0062] S3. Crush the dried chokeberries, sieve them through a 100-mesh sieve, add water with a volume 30 times that of the chokeberries, stir and extract at 90 °C and 400 rpm for 0.5 h, filter to obtain the filtrate, and concentrate it to 1 / 5 of its original volume to obtain the chokeberry extract;

[0063] S4. Crush the dried black tea, sieve it through a 100-mesh sieve, add water with a volume 50 times that of the black tea, stir and extract at 100 °C and 400 rpm for 0.5 h, filter to obtain the filtrate, and concentrate it to 1 / 20 of its original volume to obtain the black tea extract;

[0064] S5. Mix and stir 90 parts by mass of citrus fruit filtrate, 1 part of flammulina velutipes fiber, 1 part of soy protein isolate, 5 parts of chokeberry extract, and 3 parts of black tea extract according to the parts by mass, and perform spray drying. The inlet temperature of the spray drying is 220 °C, and the outlet temperature is 110 °C to obtain citrus powder (with a water content of 5.7 wt%).

[0065] Use this citrus powder to make tablets. Add 10 parts by mass of carboxymethyl cellulose to 90 parts by mass of citrus powder, granulate, and press into tablets with a mass of 0.2 g per tablet containing the citrus powder composition.

[0066] Comparative Example 2

[0067] A composition containing citrus polyphenols and citrus powder for burning fat and losing weight, including 90 parts by mass of citrus powder and 5 parts by mass of compound probiotics (1*10^8 cfu / g) according to the parts by mass.

[0068] The preparation method of this citrus powder composition is as follows:

[0069] S1. Break and homogenize the citrus fruits with peel, and filter to obtain the citrus fruit filtrate;

[0070] S2. Crush the dried chokeberries, sieve them through a 100-mesh sieve, add water with a volume 30 times that of the chokeberries, stir and extract at 90 °C and 400 rpm for 0.5 h, filter to obtain the filtrate, and concentrate it to 1 / 5 of its original volume to obtain the chokeberry extract;

[0071] S3. Crush the dried black tea, sieve it through a 100-mesh sieve, add water with a volume 50 times that of the black tea, stir and extract at 100 °C and 400 rpm for 0.5 h, filter to obtain the filtrate, and concentrate it to 1 / 20 of its original volume to obtain the black tea extract;

[0072] S4. Mix and stir 90 parts by mass of citrus fruit filtrate, 2 parts of soy protein isolate, 5 parts of chokeberry extract, and 3 parts of black tea extract according to the parts by mass, and perform spray drying. The inlet temperature of the spray drying is 220 °C, and the outlet temperature is 110 °C to obtain citrus powder.

[0073] S5. Mix 5 parts by mass of probiotics and 90 parts by mass of citrus powder to obtain the citrus powder composition.

[0074] The citrus powder composition is applied to make tablets. 5 parts by mass of carboxymethyl cellulose is added, and after granulation and tableting, tablets containing 0.2 g of the citrus powder composition per tablet are made.

[0075] Comparative Example 3

[0076] A composition containing citrus polyphenols and citrus powder for burning fat and losing weight, by mass, includes 90 parts by mass of citrus powder and 5 parts by mass of compound probiotics (1×10^8 cfu / g).

[0077] The preparation method of the citrus powder composition is as follows:

[0078] S1. The citrus fruits with peel are broken, homogenized, and filtered to obtain citrus fruit filtrate.

[0079] S2. Fresh Flammulina velutipes is added to 10 times its volume of water, stirred and extracted at 80 °C at 200 rpm for 1 h, filtered through a 400-mesh sieve, washed with 10 times its volume of water, filtered through a 400-mesh sieve to obtain solids, dried at 45 °C to obtain Flammulina velutipes crude fiber, pulverized, and sieved through a 40-mesh sieve to obtain Flammulina velutipes fiber (water content ≤ 8 wt%).

[0080] S3. The dried black tea is pulverized, sieved through a 100-mesh sieve, added to 50 times its volume of water, stirred and extracted at 100 °C at 400 rpm for 0.5 h, filtered, and the filtrate is concentrated to obtain black tea extract.

[0081] S4. By mass, 90 parts of citrus fruit filtrate, 1 part of Flammulina velutipes fiber, 1 part of soy protein isolate, 5 parts of water, and 8 parts of black tea extract are mixed and stirred, and then spray-dried. The inlet temperature of spray drying is 220 °C, and the outlet temperature is 110 °C to obtain citrus powder (water content is 5.7 wt%).

[0082] S5. 5 parts by mass of probiotics and 90 parts by mass of citrus powder are mixed to obtain the citrus powder composition.

[0083] The citrus powder composition is applied to make tablets. 5 parts by mass of carboxymethyl cellulose is added, and after granulation and tableting, tablets containing 0.2 g of the citrus powder composition per tablet are made.

[0084] Comparative Example 4

[0085] A composition containing citrus polyphenols and citrus powder for burning fat and losing weight, by mass, includes 90 parts by mass of citrus powder and 5 parts by mass of compound probiotics (1×10^8 cfu / g).

[0086] The preparation method of the citrus powder composition is as follows:

[0087] S1. Break and homogenize the citrus fruits with peel, and then filter to obtain the citrus fruit filtrate;

[0088] S2. Add fresh Flammulina velutipes to 10 times its volume of water, stir and extract at 80 °C at 200 rpm for 1 h, filter through a 400-mesh sieve, wash with 10 times its volume of water, filter through a 400-mesh sieve to obtain the solid matter, dry at 45 °C to obtain the crude fiber of Flammulina velutipes, crush it, and sieve through a 40-mesh sieve to obtain Flammulina velutipes fiber (with a water content of 6.5 wt%);

[0089] S3. Crush the dried Aronia melanocarpa, sieve through a 100-mesh sieve, add 30 times its volume of water, stir and extract at 90 °C at 400 rpm for 0.5 h, filter and concentrate the filtrate to 1 / 5 of its original volume to obtain the Aronia melanocarpa extract;

[0090] S4. Mix and stir 90 parts by mass of the citrus fruit filtrate, 1 part by mass of Flammulina velutipes fiber, 1 part by mass of soy protein isolate, 8 parts by mass of the Aronia melanocarpa extract, and 3 parts by mass of water, and then perform spray drying. The inlet temperature of spray drying is 220 °C and the outlet temperature is 110 °C to obtain citrus powder (with a water content of 5.7 wt%);

[0091] S5. Mix 5 parts by mass of probiotics and 90 parts by mass of the citrus powder to obtain the citrus powder composition.

[0092] Apply the citrus powder composition to make tablets, add 5 parts by mass of carboxymethyl cellulose, granulate, and press into tablets containing 0.2 g of the citrus powder composition per tablet.

[0093] Testing of the content index of citrus powder

[0094] The determination of dietary fiber content in citrus powder refers to GB5009.88-2023 "National Food Safety Standard - Determination of Dietary Fiber in Foods", the determination of total polyphenol content refers to GB / T 44349-2024 "Detection of Total Polyphenols in Bee Pollen - Folin-Ciocalteu Reagent Colorimetric Method", and the determination of Vc content refers to GB 5009.86-2016 "National Food Safety Standard - Determination of Ascorbic Acid in Foods".

[0095] Table 1 Composition index of citrus powder

[0096]

[0097] Efficacy test

[0098] Test method: The blank control group took placebo, 2 capsules per day; the examples and comparative examples were continuously taken for 8 weeks according to the above method. During the whole experiment, the diet was not controlled, no exercise was deliberately increased, and other weight loss foods and drugs were prohibited. 80 obese subjects with body mass index evaluation were selected and randomly divided into 8 groups. Before and after the experiment, an InBody body composition analyzer (model 770) was used to measure the body weight, body fat percentage, and body mass index of the subjects. *, ** indicate significant differences before and after the experiment (P < 0.05), and extremely significant differences before and after the experiment (P < 0.01).

[0099] Table 2 Changes in the body weight of the subjects

[0100]

[0101] Table 3 Changes in the body fat of the subjects

[0102]

[0103] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A citrus powder composition containing citrus polyphenols for burning fat and losing weight, characterized in that, It includes citrus powder and compound probiotics; the compound probiotics include Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium lactis and Lactobacillus casei; The preparation method of this composition includes: S1. Break and homogenize the citrus fruits with peel, and filter to obtain the citrus fruit filtrate; S2. Add fresh Flammulina velutipes to 5 - 10 times its volume of water, extract at 80 - 90 °C, filter, wash, separate, dry to obtain the crude fiber of Flammulina velutipes, pulverize, and sieve through a 20 - 40 mesh to obtain Flammulina velutipes fiber; S3. Pulverize the dried Aronia melanocarpa, sieve through a 100 - 200 mesh, add 30 - 50 times its volume of water, extract at 70 - 90 °C for 0.5 - 1 h, filter and take the filtrate for concentration to 1 / 3 - 1 / 5 of its volume to obtain the Aronia melanocarpa extract; S4. Pulverize the dried black tea, sieve through a 100 - 200 mesh, add 30 - 50 times its volume of water, extract at 80 - 100 °C for 0.5 - 1 h, filter and take the filtrate for concentration to obtain the black tea extract; S5. Mix 80 - 90 parts by mass of the citrus fruit filtrate, 1 - 2 parts by mass of Flammulina velutipes fiber, 1 - 5 parts by mass of soy protein isolate, 5 - 8 parts by mass of Aronia melanocarpa extract, and 2 - 5 parts by mass of black tea extract, and perform spray drying to obtain citrus powder; S6. Mix 1 - 10 parts by mass of compound probiotics and 90 parts by mass of citrus powder to obtain the citrus powder composition.

2. The citrus powder composition containing citrus polyphenols according to claim 1, wherein By mass fraction, the dietary fiber content of the said citrus powder is ≥ 40%, the total polyphenol content is ≥ 5%, and the Vc content is ≥ 2%.

3. The citrus powder composition containing citrus polyphenols according to claim 1, wherein The caffeine content of the said black tea extract is ≥ 0.5%.

4. The citrus powder composition containing citrus polyphenols according to claim 1, wherein For the said spray drying, the inlet temperature is 180 - 220 °C, and the outlet temperature is 105 - 110 °C.

5. Use of a citrus powder composition containing citrus polyphenols for burning fat and losing weight according to any one of claims 1-4, characterized in that, It is used for preparing weight - loss drugs.

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