Multi-layer sampling device for dynamically researching plant rhizosphere environment change

Abstract

The invention belongs to the technical field of plants, and particularly relates to a multi-layer sampling device for dynamically researching plant rhizosphere environment change, which comprises a hexagonal bottom plate, a first hexagonal coaming is integrally arranged at the top of the hexagonal bottom plate, and a second hexagonal coaming is integrally arranged at the bottom of the hexagonal bottom plate; a second hexagonal coaming is integrally arranged at the top of the hexagonal bottom plate located on the inner side of the first hexagonal coaming, a third hexagonal coaming is integrally arranged at the top of the hexagonal bottom plate located on the inner side of the second hexagonal coaming, and a fourth hexagonal coaming is integrally arranged at the top of the hexagonal bottom plate located on the inner side of the third hexagonal coaming. The device has the advantages that a plurality of longitudinal sampling areas can be formed on the hexagonal bottom plate, so that dynamic sampling can be carried out according to the sampling time, and the plant root system is prevented from being damaged; in addition, the multi-layer sampling device is simple in structure and can be repeatedly used for multiple times, and research of researchers is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of plants, in particular to a multi-layer sampling device for dynamically studying changes in plant rhizosphere environment. Background Art

[0002] The rhizosphere refers to the part of the micro-domain soil area that is affected by the activity of plant roots and is different from the soil in physical, chemical and biological properties. The scope of the rhizosphere is very small, generally within a few millimeters from the surface of the root axis. It exists not only in the radial direction from the root surface to the original soil body, but also in the longitudinal direction from the root base to the root tip. Since it is difficult to study the rhizosphere micro-domain under field conditions, many researchers use self-made root boxes to simulate the study of rhizosphere and non-rhizosphere soils, such as the root box device used in the article published by Yao Jianhua et al. in "Chinese Agricultural Science" Vol. 43, No. 4, 2010, and He et al. in "Soil Biology & Biochemistry" Vol. 37, 2005. The volume of the root box: length × width × height = 150mm × 140mm × 230mm. It is divided into three areas with a 30μm nylon mesh, the middle area with a width of 20mm is the rhizosphere, and the areas with a width of 60mm on both sides are the non-rhizosphere. The growth space of plant roots is: length × width × height = 150mm × 20mm × 230mm. Therefore, its radial growth can only extend in two directions, which does not conform to the actual environment of extending and growing in all directions under field conditions.

[0003] The existing patent CN103293035A discloses a root box test device suitable for studying plant rhizosphere micro-domains. Although the patent can solve the above problems, there is only one large sampling area in the patent, which makes it difficult to perform dynamic sampling according to the sampling time, which has limitations. Therefore, a multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment is invented. Summary of the invention

[0004] In view of the above problems and / or the problems existing in the existing multi-layer sampling device for dynamically studying the changes in the plant rhizosphere environment, the present invention is proposed.

[0005] Therefore, the object of the present invention is to provide a multi-layer sampling device for dynamically studying changes in plant rhizosphere environment, which can solve the above-mentioned existing problems.

[0006] To solve the above technical problems, according to one aspect of the present invention, the present invention provides the following technical solutions: A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment, comprising a hexagonal bottom plate, a first hexagonal enclosure plate integrated on the top of the hexagonal bottom plate, a second hexagonal enclosure plate integrated on the top of the hexagonal bottom plate located inside the first hexagonal enclosure plate, a third hexagonal enclosure plate integrated on the top of the hexagonal bottom plate located inside the second hexagonal enclosure plate, and a fourth hexagonal enclosure plate integrated on the top of the hexagonal bottom plate located inside the third hexagonal enclosure plate; A plurality of first boxes are inserted between the first hexagonal enclosure and the second hexagonal enclosure, a plurality of second boxes are inserted between the second hexagonal enclosure and the third hexagonal enclosure, and a plurality of third boxes are inserted between the third hexagonal enclosure and the fourth hexagonal enclosure.

[0007] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, the space between the first hexagonal enclosure and the second hexagonal enclosure is set as a soil lifting cavity, and six groups of first boxes are inserted into the soil lifting cavity.

[0008] As a preferred solution of the multi-layer sampling device for dynamically studying the changes in the plant rhizosphere environment described in the present invention, the space between the second hexagonal enclosure and the third hexagonal enclosure is set as a peri-root cavity, and six groups of second boxes are inserted in the peri-root cavity.

[0009] As a preferred solution of the multi-layer sampling device for dynamically studying the changes in the plant rhizosphere environment described in the present invention, the space between the third hexagonal enclosure and the fourth hexagonal enclosure is set as a rhizosphere cavity, and six groups of third boxes are inserted in the rhizosphere cavity.

[0010] As a preferred solution of the multi-layer sampling device for dynamically studying the changes in the plant rhizosphere environment described in the present invention, the inner cavity space of the fourth hexagonal enclosure is set as a planting cavity for planting plants.

[0011] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, the second hexagonal enclosure is provided with six groups of first through holes, the third hexagonal enclosure is provided with six groups of second through holes, and the fourth hexagonal enclosure is provided with six groups of third through holes.

[0012] As a preferred solution of the multi-layer sampling device for dynamically studying changes in the plant rhizosphere environment described in the present invention, each group of the first box bodies has a fourth through hole on the inner side, each group of the second box bodies has a fifth through hole on the outer side, each group of the second box bodies has a sixth through hole on the inner side, each group of the third box bodies has a seventh through hole on the outer side, and each group of the third box bodies has an eighth through hole on the inner side.

[0013] As a preferred solution of the multi-layer sampling device for dynamically studying the change of plant rhizosphere environment described in the present invention, wherein: the fourth through hole is aligned with the first through hole, the first through hole is aligned with the fifth through hole, the fifth through hole is aligned with the sixth through hole, the sixth through hole is aligned with the second through hole, the second through hole is aligned with the seventh through hole, the seventh through hole is aligned with the eighth through hole, and the eighth through hole is aligned with the third through hole; The first through hole, the second through hole, the third through hole, the fourth through hole, the fifth through hole, the sixth through hole, the seventh through hole and the eighth through hole are all provided with a spacing membrane, and the spacing membrane is set to a 30-micron nylon membrane.

[0014] As a preferred solution of the multi-layer sampling device for dynamically studying the change of plant rhizosphere environment described in the present invention, it also includes: A lifting assembly is provided to facilitate lifting out the first box body, the second box body and the third box body, and the inner surfaces of the first box body, the second box body and the third box body are all provided with lifting assemblies.

[0015] As a preferred solution of the multi-layer sampling device for dynamically studying the changes in the plant rhizosphere environment described in the present invention, the pulling component includes: A groove, wherein the top of the inner surface of the first box body, the second box body and the third box body are all provided with a groove; Support blocks are fixedly mounted on both sides of the top of the inner cavity of the groove; A rotating shaft, the rotating shaft being rotatably connected to the supporting block via a bearing; A square plate, wherein the square plate is fixedly installed between the two sets of rotating shafts; A torsion spring, wherein the torsion spring is sleeved on the rotating shaft, and two ends of the torsion spring are fixedly connected to the support block and the square plate respectively; A notch is formed on the top of the inner surfaces of the first box body, the second box body and the third box body. The notch is located directly below the groove, and the notch is connected to the groove.

[0016] Compared with existing technologies: By sequentially arranging a first hexagonal enclosure, a second hexagonal enclosure, a third hexagonal enclosure and a fourth hexagonal enclosure on the top of the hexagonal base plate, and by inserting a plurality of first boxes, second boxes and third boxes in the space between the first hexagonal enclosure, the second hexagonal enclosure, the third hexagonal enclosure and the fourth hexagonal enclosure, it is possible to form a plurality of longitudinal sampling areas on the hexagonal base plate, thereby enabling dynamic sampling according to the sampling time and ensuring that the plant root system is not damaged; in addition, the multi-layer sampling device provided by the present invention not only has a simple structure, but can also be reused many times, which facilitates the research of scientific researchers. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the structure of the present invention; Figure 2 It is a top view schematic diagram of the structure of the present invention; Figure 3 It is a top view cross-sectional schematic diagram of the structure of the present invention; Figure 4 It is a top view cross-sectional schematic diagram of the local structure of the present invention Figure 5 It is a front view schematic diagram of the structure of the present invention; Figure 6 It is a top view schematic diagram of the lifting assembly structure of the present invention; Figure 7 For the present invention Figure 6 A schematic diagram of the structure enlargement in the middle; Figure 8 It is a front view schematic diagram of the lifting assembly structure of the present invention.

[0018] In the figure: hexagonal base plate 10, first hexagonal enclosure plate 20, second hexagonal enclosure plate 30, first through hole 31, third hexagonal enclosure plate 40, second through hole 41, fourth hexagonal enclosure plate 50, third through hole 51, first box body 60, fourth through hole 61, second box body 70, fifth through hole 71, sixth through hole 72, third box body 80, seventh through hole 81, eighth through hole 82, groove 90, support block 91, rotating shaft 92, square plate 93, torsion spring 94, notch 95, spacer membrane 100. DETAILED DESCRIPTION

[0019] In order to make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings. Example

[0020] The present invention provides a multi-layer sampling device for dynamically studying the changes in the plant rhizosphere environment. Figure 1-Figure 8, comprising a hexagonal bottom plate 10, a first hexagonal enclosure 20 is integrated on the top of the hexagonal bottom plate 10, a second hexagonal enclosure 30 is integrated on the top of the hexagonal bottom plate 10 located inside the first hexagonal enclosure 20, a third hexagonal enclosure 40 is integrated on the top of the hexagonal bottom plate 10 located inside the second hexagonal enclosure 30, and a fourth hexagonal enclosure 50 is integrated on the top of the hexagonal bottom plate 10 located inside the third hexagonal enclosure 40; A plurality of first boxes 60 are inserted between the first hexagonal enclosure 20 and the second hexagonal enclosure 30 , a plurality of second boxes 70 are inserted between the second hexagonal enclosure 30 and the third hexagonal enclosure 40 , and a plurality of third boxes 80 are inserted between the third hexagonal enclosure 40 and the fourth hexagonal enclosure 50 .

[0021] The space between the first hexagonal enclosure 20 and the second hexagonal enclosure 30 is set as a soil lifting cavity, in which six groups of first box bodies 60 are inserted; the space between the second hexagonal enclosure 30 and the third hexagonal enclosure 40 is set as a root cavity, in which six groups of second box bodies 70 are inserted; the space between the third hexagonal enclosure 40 and the fourth hexagonal enclosure 50 is set as a rhizosphere cavity, in which six groups of third box bodies 80 are inserted; the inner cavity space of the fourth hexagonal enclosure 50 is set as a planting cavity for planting plants.

[0022] The second hexagonal enclosure 30 is provided with six groups of first through holes 31, the third hexagonal enclosure 40 is provided with six groups of second through holes 41, the fourth hexagonal enclosure 50 is provided with six groups of third through holes 51, the inner side of each group of first box bodies 60 is provided with fourth through holes 61, the outer side of each group of second box bodies 70 is provided with fifth through holes 71, the inner side of each group of second box bodies 70 is provided with sixth through holes 72, and the outer side of each group of third box bodies 80 is provided with The seventh through hole 81, the inner side of each group of the third box body 80 is provided with an eighth through hole 82, the fourth through hole 61 is aligned with the first through hole 31, the first through hole 31 is aligned with the fifth through hole 71, the fifth through hole 71 is aligned with the sixth through hole 72, the sixth through hole 72 is aligned with the second through hole 41, the second through hole 41 is aligned with the seventh through hole 81, the seventh through hole 81 is aligned with the eighth through hole 82, and the eighth through hole 82 is aligned with the third through hole 51; A spacing membrane 100 is disposed in each of the first through hole 31 , the second through hole 41 , the third through hole 51 , the fourth through hole 61 , the fifth through hole 71 , the sixth through hole 72 , the seventh through hole 81 and the eighth through hole 82 , and the spacing membrane 100 is a 30-micron nylon membrane.

[0023] When used, the specific steps are as follows: Step 1: air-dry and sieve the soil sample retrieved from the field, and then put it into the first box body 60, the second box body 70, the third box body 80 and the planting cavity; Step 2: Plant the plants in the planting cavity; Step 3: During the growth and development of the plant, relevant information or data of the rhizosphere soil and non-rhizosphere soil can be obtained by taking out the first box body 60, the second box body 70 or the third box body 80, thereby promoting the normal development of relevant research on the rhizosphere micro-domain. Example

[0024] In order to facilitate lifting out the first box body 60 , the second box body 70 and the third box body 80 , lifting components are provided on the inner surfaces of the first box body 60 , the second box body 70 and the third box body 80 on the basis of Embodiment 1.

[0025] The lifting assembly includes: a groove 90, a support block 91, a rotating shaft 92, a square plate 93, a torsion spring 94, and a notch 95; The top of the inner surface of the first box body 60, the second box body 70 and the third box body 80 are all provided with a groove 90, and support blocks 91 are fixedly installed on both sides of the top of the inner cavity of the groove 90. The rotating shaft 92 is rotatably connected to the support block 91 through a bearing, and the square plate 93 is fixedly installed between the two groups of rotating shafts 92. The torsion spring 94 is sleeved on the rotating shaft 92, and the two ends of the torsion spring 94 are fixedly connected to the support block 91 and the square plate 93 respectively. The top of the inner surface of the first box body 60, the second box body 70 and the third box body 80 are all provided with a notch 95, the notch 95 is located directly below the groove 90, and the notch 95 and the groove 90 are connected.

[0026] When used, the specific steps are as follows: When the box body needs to be taken out, the square plate 93 is first rotated from the notch 95. At this time, the torsion spring 94 will be deformed until the square plate 93 contacts the top of the groove 90 after rotation. At this time, the square plate 93 and the groove 90 will be in a vertical state. Then, the square plate 93 is pulled upward to be able to lift the box body out. After that, the square plate 93 will be restored to its original position under the action of the torsion spring 94.

[0027] Although the present invention has been described above with reference to the embodiments, various modifications may be made thereto and parts thereof may be replaced by equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention may be used in combination with each other in any manner, and the fact that these combinations are not exhaustively described in this specification is only for the sake of omitting space and saving resources. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment, characterized in that: Comprising a hexagonal bottom plate (10), a first hexagonal enclosure plate (20) being integrally provided on the top of the hexagonal bottom plate (10), a second hexagonal enclosure plate (30) being integrally provided on the top of the hexagonal bottom plate (10) located inside the first hexagonal enclosure plate (20), a third hexagonal enclosure plate (40) being integrally provided on the top of the hexagonal bottom plate (10) located inside the second hexagonal enclosure plate (30), and a fourth hexagonal enclosure plate (50) being integrally provided on the top of the hexagonal bottom plate (10) located inside the third hexagonal enclosure plate (40); A plurality of first boxes (60) are inserted between the first hexagonal enclosure (20) and the second hexagonal enclosure (30), a plurality of second boxes (70) are inserted between the second hexagonal enclosure (30) and the third hexagonal enclosure (40), and a plurality of third boxes (80) are inserted between the third hexagonal enclosure (40) and the fourth hexagonal enclosure (50).

2. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The space between the first hexagonal enclosure plate (20) and the second hexagonal enclosure plate (30) is set as a soil lifting cavity, and six groups of first box bodies (60) are inserted into the soil lifting cavity.

3. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The space between the second hexagonal enclosure plate (30) and the third hexagonal enclosure plate (40) is set as a root cavity, and six groups of second box bodies (70) are inserted into the root cavity.

4. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The space between the third hexagonal enclosure plate (40) and the fourth hexagonal enclosure plate (50) is set as a rhizosphere cavity, and six sets of third box bodies (80) are inserted into the rhizosphere cavity.

5. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The inner cavity space of the fourth hexagonal enclosure plate (50) is set as a planting cavity for planting plants.

6. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: The second hexagonal enclosure plate (30) is provided with six groups of first through holes (31), the third hexagonal enclosure plate (40) is provided with six groups of second through holes (41), and the fourth hexagonal enclosure plate (50) is provided with six groups of third through holes (51).

7. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 6, characterized in that: Each group of the first box bodies (60) has a fourth through hole (61) on its inner side, each group of the second box bodies (70) has a fifth through hole (71) on its outer side, each group of the second box bodies (70) has a sixth through hole (72) on its inner side, each group of the third box bodies (80) has a seventh through hole (81) on its outer side, and each group of the third box bodies (80) has an eighth through hole (82) on its inner side.

8. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 7, characterized in that: The fourth through hole (61) is aligned with the first through hole (31), the first through hole (31) is aligned with the fifth through hole (71), the fifth through hole (71) is aligned with the sixth through hole (72), the sixth through hole (72) is aligned with the second through hole (41), the second through hole (41) is aligned with the seventh through hole (81), the seventh through hole (81) is aligned with the eighth through hole (82), and the eighth through hole (82) is aligned with the third through hole (51); The first through hole (31), the second through hole (41), the third through hole (51), the fourth through hole (61), the fifth through hole (71), the sixth through hole (72), the seventh through hole (81) and the eighth through hole (82) are all provided with a spacing membrane (100), and the spacing membrane (100) is set to be a nylon membrane with a thickness of 30 micrometers.

9. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 1, characterized in that: Also includes: A lifting assembly is provided for facilitating lifting out the first box body (60), the second box body (70) and the third box body (80), and the inner surfaces of the first box body (60), the second box body (70) and the third box body (80) are all provided with the lifting assembly.

10. A multi-layer sampling device for dynamically studying changes in plant rhizosphere environment according to claim 9, characterized in that: The lifting component comprises: A groove (90), wherein the top ends of the inner surfaces of the first box body (60), the second box body (70) and the third box body (80) are all provided with a groove (90); A support block (91), wherein support blocks (91) are fixedly mounted on both sides of the top end of the inner cavity of the groove (90); A rotating shaft (92), the rotating shaft (92) being rotatably connected to the supporting block (91) via a bearing; A square plate (93), wherein the square plate (93) is fixedly mounted between the two sets of rotating shafts (92); a torsion spring (94), wherein the torsion spring (94) is sleeved on the rotating shaft (92), and two ends of the torsion spring (94) are respectively fixedly connected to the support block (91) and the square plate (93); A notch (95) is provided at the top of the inner surface of each of the first box body (60), the second box body (70) and the third box body (80). The notch (95) is located directly below the groove (90), and the notch (95) and the groove (90) are connected.

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