Multifunctional shaking culture integrated shaking table and method based on cell culture

Abstract

The invention discloses a multifunctional shaking culture integrated shaking table and method based on cell culture, and relates to the technical field of enzyme biological cells, a split type shaking platform used for placing a container is arranged in a machine body, the shaking platform is composed of a plurality of shaking plates, and clamping pieces are arranged on the upper portions of the shaking plates to clamp the container; a driving module connected with the oscillation platform is arranged in the machine body and comprises an oscillation unit and a connecting unit, the connecting unit is connected with the oscillation unit and the oscillation plates, the connecting unit configures two transmission states of the adjacent oscillation plates through a differential mechanism, and the differential mechanism configures the driving module to drive the oscillation plates to oscillate at the same speed or at the differential speed. The oscillation platform is divided into a plurality of oscillation plates, when enzyme biological cells are oscillated, the enzyme biological cells needing different oscillation parameters are placed on different oscillation plates, and oscillation of multiple enzyme biological cells can be carried out in the same shaking table through the oscillation plates with different oscillation frequencies.

Description

Technical Field

[0001] The invention relates to the technical field of enzyme biological cells, in particular to an integrated shaking table and method for realizing enzyme activity determination, enzyme reaction experiments, enzyme biological cell separation, purification and cultivation through oscillation. Background Art

[0002] Constant temperature shaker is widely used in enzyme biological research, mainly including the following aspects: The constant temperature shaker performs enzyme activity determination at a constant temperature to ensure the stability of experimental conditions and thus obtain accurate determination results.

[0003] When conducting enzymatic reaction experiments, the constant temperature shaker provides constant temperature and oscillation conditions, which helps to evenly mix the enzyme and substrate and improve the reaction efficiency.

[0004] Oscillation can promote the separation of enzymes from other proteins and improve purification efficiency.

[0005] By maintaining constant temperature and shaking conditions, the shelf life of the enzyme is extended and its activity is maintained.

[0006] Announcement No. CN114774276B discloses a thermostatic shaker movement assembly and a thermostatic shaker, including a bottom plate, a shaker, an eccentric drive mechanism and a follower mechanism, wherein the shaker is arranged directly above the bottom plate; the eccentric drive mechanism is arranged on the bottom plate, capable of driving the shaker to move, and the output end of the eccentric shaft of the eccentric drive mechanism is rotatably connected to the first bearing seat on the shaker through a bearing; the follower mechanism includes a translation component, a side rocker and a connecting rod, the translation component is located between the bottom plate and the shaker, and can move horizontally and / or vertically between the two; there are two side rockers, which are arranged in parallel, and one end of the side rocker is hinged to the first side of the translation component; the connecting rod is hinged to the other end of the two side rockers to form a parallelogram, and the connecting rod is fixedly connected to the first bearing seat. Through the design of the follower mechanism, it can only translate but not rotate, thereby preventing the first bearing seat from rotating during the operation of the shaker.

[0007] The shaker is driven by a motor, and the transmission mechanism is used to drive the shaker platform to move. Common transmission mechanisms include: Crank-connecting rod mechanism: converts rotational motion into horizontal reciprocating motion.

[0008] Eccentric mechanism: The eccentric wheel rotates to drive the platform to oscillate in a circular or elliptical shape.

[0009] Ball screw: The rotation of the screw drives the platform to move in a straight line, which is suitable for precision-controlled shakers.

[0010] However, there are some corresponding problems with the above driving method. Since the height of the internal liquid is different and the containers are different, the stability of the liquid is poor when oscillating by the above mechanical driving method. Therefore, a specific container and control of the liquid level are required during oscillation. Summary of the invention

[0011] One of the purposes of the present invention is to provide a multifunctional integrated shaking table and method for cell culture, which controls the oscillation parameters of the oscillation platform by obtaining the state of the liquid on the oscillation platform, avoids the liquid inside the container from spilling out due to excessive oscillation parameters, and controls the state of the liquid more accurately according to the container.

[0012] To achieve the above objectives, the present invention is implemented through the following technical solutions: A multifunctional oscillating culture integrated shaker based on cell culture, comprising a body that can be placed on the ground and is equipped with an observation window, a split oscillating platform for placing a container is arranged inside the body, the oscillating platform is composed of a plurality of oscillating plates, and a clamping piece is arranged on the upper part of the oscillating plate to clamp the container; A driving module connected to the oscillating platform is configured inside the machine body, the driving module includes an oscillating unit and a connecting unit, the connecting unit connects the oscillating unit and the oscillating plate, the connecting unit configures two transmission states of adjacent oscillating plates through a differential, and the differential is configured so that the driving module drives multiple oscillating plates to oscillate at the same speed or at a differential speed; The collecting device is installed inside the machine body and collects the liquid level changes of the container during the oscillation process.

[0013] In one or more embodiments of the present invention, the body further comprises a retractable cover, a cover for observation and sealing is hinged to the outside of the cover, and the cover can be swung open; The cover body can be retracted and installed inside the machine body, and a clamping plate is arranged outside the machine body for supporting and limiting the cover body. When the cover body is retracted, the volume of the rocking table is smaller than the volume of the rocking table when the cover body is supported.

[0014] In one or more embodiments of the present invention, a first connecting member, a second connecting member, and a transmission member connecting the first connecting member and the second connecting member and adjusting the transmission ratio of the first connecting member and the second connecting member are arranged inside the differential housing; The first connecting member and the second connecting member are respectively installed at the bottom of two adjacent oscillating plates and are configured to realize the movement of the two adjacent oscillating plates in a manner that the first connecting member is used as an active member and the second connecting member is used as a driven member.

[0015] In one or more embodiments of the present invention, the transmission member includes: The first slider and the second slider are both arranged inside the differential and connected to the first connecting member and the second connecting member respectively, and the first connecting member and the second connecting member move synchronously with the first slider and the second slider; The communication pipeline is arranged inside the differential housing, and the first slider and the second slider slide to change the medium inside the communication pipeline; A pressure plate is arranged inside the differential housing. A magnetic component is configured at one end of the pressure plate away from the first slider or the second slider to adjust the position of the pressure plate. Changes in the position of the pressure plate change the cross-sectional area of ​​the first slider or the second slider slot. When the cross-sectional area of ​​the pressure plate changes, the cross-sectional area of ​​the first slider and the second slider extending to the inner end of the slot changes accordingly, and a connecting pipe connects the slot.

[0016] In one or more embodiments of the present invention, the first slider and the second slider are both provided with a folding plate supported by a compression spring and in contact with the outer wall of the pressing plate, and when the pressing plate is squeezed, the folding plate is also squeezed, and the pressing plate and the folding plate move synchronously; An inlet and an outlet are arranged on both sides of the chute where the first slider and the second slider are installed, and the inlet and outlet on one side cannot be opened at the same time. A sealing member moving along the movement direction of the first slider or the second slider is arranged on the outer side of the chute, and the sealing member extends to the chute to seal the inlet or outlet, and the outlets or inlets on both sides of the chute cannot be opened at the same time; The connecting pipeline connects the outlet of the slide groove installed by the first slider and the inlet of the slide groove installed by the second slider, and the inlet of the slide groove installed by the first slider and the outlet of the slide groove installed by the second slider.

[0017] In one or more embodiments of the present invention, the first connecting member and the second connecting member both include: The connecting plate is installed on the bottom of the oscillating plate through a connecting structure, and a connecting rod is arranged at one end of the connecting plate to connect the first slider and the connecting plate or the second slider and the connecting plate.

[0018] In one or more embodiments of the present invention, the first connecting member and the second connecting member are configured as a four-bar mechanism, and the linear reciprocating motion of the first slider or the second slider is converted into a circular motion through the four-bar mechanism.

[0019] In one or more embodiments of the present invention, the clamping member comprises: A placement rack is installed on the upper part of the oscillation plate, the inner side of the placement rack is provided with an arc-shaped soft board, both ends of the arc-shaped soft board are provided with magnetic blocks, and the magnetic absorption between adjacent magnetic blocks shrinks the cross-sectional area of ​​the arc-shaped soft board; The elastic body is fixed to one side of the arc-shaped soft plate that is attached to the container, and a cavity is arranged inside the elastic body.

[0020] In one or more embodiments of the present invention, the oscillating unit comprises: The electromagnet is fixed inside the machine body, and a guide frame is arranged on the outer wall of the oscillating plate close to the electromagnet, and a guide magnet is arranged inside the guide frame, and the guide magnet moves inside the guide frame; The positioning magnets are fixed to the two ends of the inner wall of the guide frame to support the guide magnets and prevent the guide magnets from contacting the positioning magnets.

[0021] The present application also provides an integrated shaking table oscillation method, which is used for the above-mentioned integrated shaking table, comprising the following steps: Determine the oscillation frequency of the enzyme biological cell to be oscillated, and place the container inside the clamp; The oscillation unit drives one of the oscillation plates to oscillate, and the oscillation plate is connected to other oscillation plates through the connection unit to oscillate synchronously; The oscillation frequency of each oscillation plate is adjusted by a differential; During the oscillation process, the acquisition device obtains the container liquid level image during the oscillation process, and analyzes the container liquid level image to determine the fluctuation of the liquid level during the oscillation process.

[0022] Through the above technical solution, the present invention has the following beneficial effects: 1. The present application divides the oscillation platform into multiple oscillation plates. When oscillating enzyme biological cells, enzyme biological cells requiring different oscillation parameters are placed on different oscillation plates. Oscillation plates with different oscillation frequencies can be used to oscillate multiple enzyme biological cells in the same shaker, further improving the adaptability of the shaker.

[0023] 2. By using an adjustable differential, the transmission ratio between the first connecting member and the second connecting member can be dynamically adjusted, so that the two oscillation plates connected by the first connecting member and the second connecting member can generate different oscillation frequencies, so that multiple oscillation plates oscillate synchronously but present different oscillation frequencies.

[0024] 3. The setting of the differential can realize the same-speed movement and differential movement of two adjacent oscillation plates, that is, the oscillation frequency of two adjacent oscillation plates can be adjusted at will, so as to change the vibration frequency of enzyme biological cells at different positions, and oscillate with multiple vibration frequencies in the same shaker.

[0025] 4. By using the acquisition equipment to collect the liquid level status of the container during the oscillation of the shaker, it can be determined whether the liquid level will spill under the vibration frequency. By collecting images collected by the acquisition equipment, the oscillation frequency of the container that may spill can be reduced to ensure the stability of the liquid inside the container.

[0026] Other features and advantages of the present invention will be described in the following description, and part of them will become obvious from the description, or will be understood by practicing the present invention. The purpose and other advantages of the present invention can be realized and obtained by the structures particularly pointed out in the written description and the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A perspective view of the present invention; Figure 2 It is a schematic diagram of the internal structure of the present invention; Figure 3 This is a schematic diagram of the structure of removing the clamping member of the present invention; Figure 4 It is a schematic diagram of the oscillating platform of the present invention; Figure 5 It is a schematic diagram of the bottom of the oscillating platform of the present invention; Figure 6 It is a schematic diagram of the oscillating plate of the present invention; Figure 7 It is a schematic diagram of the connection unit of the present invention; Figure 8 is a cross-sectional view of a differential of the present invention; Fig. 9 An exploded view of the internal structure of the differential of the present invention; Fig.10 It is a schematic diagram of the local structure of the differential of the present invention; Fig.11 It is a schematic diagram of the pressing plate structure of the present invention; Fig.12 An exploded view of the first slider or the second slider structure of the present invention; Fig.13 It is a schematic diagram of the internal structure of the guide structure of the present invention; Fig.14 It is a schematic diagram of the structure of the clamping member of the present invention; Fig.15 It is a schematic diagram of the curved soft board structure of the present invention.

[0028] In the figure: 1 body, 2 oscillation platform, 3 clamping member, 4 driving module, 5 oscillation unit, 6 connection unit, 7 acquisition device; 21 oscillating plate, 22 cover, 23 lid, 24 card plate; 31 a placement rack, 32 an arc-shaped soft board, 33 a magnetic block, 34 an elastic body; 51 electromagnet, 52 guide frame, 53 guide magnet, 54 positioning magnet; 61 first connecting member, 62 second connecting member, 63 transmission member; 611 connecting plate, 612 connecting rod; 631 first slider, 632 second slider, 633 slide groove, 634 connecting pipeline, 635 pressure plate, 636 magnetic component, 637 folding plate, 638 compression spring, 639 inlet, 6310 outlet, 6311 sealing element. DETAILED DESCRIPTION

[0029] The following will disclose multiple embodiments of the present invention with the accompanying drawings. For the purpose of clear description, many practical details will be described together in the following description. However, it should be understood that these practical details should not be used to limit the present invention. In other words, in some embodiments of the present invention, these practical details are not necessary. And if it is possible to implement, the features of different embodiments can be applied interchangeably.

[0030] Unless otherwise defined, all words (including technical and scientific terms) used herein have their usual meanings, which can be understood by those familiar with this field. Furthermore, the definitions of the above words in commonly used dictionaries should be interpreted in the content of this specification as consistent with the meanings of the relevant fields of the present invention. Unless otherwise clearly defined, these words will not be interpreted as idealized or overly formal meanings.

[0031] The following is an explanation of the relationships and terms used in this application: Parallel: The parallelism defined in this application is not limited to absolute parallelism. This definition of parallelism can be understood as basic parallelism, which allows for non-absolute parallelism due to factors such as assembly tolerance, design tolerance, and the influence of structural flatness. Small angular errors are allowed. For example, an assembly error range of less than 10 degrees can be understood as a parallel relationship.

[0032] Vertical: The verticality defined in this application is not limited to an absolute vertical intersection relationship (angle of 90 degrees). It allows for non-absolute vertical intersection relationships caused by factors such as assembly tolerance, design tolerance, and influence of structural flatness. It also allows for errors in a small angle range. For example, the assembly error range of 80 to 100 degrees can be understood as a vertical relationship.

[0033] Floor: The floor defined in this application is not limited to a certain material or region, but only a platform for carrying this application, and stacking, tilting, and flatness changes are allowed. For example, cement floor, tile floor, work platform, etc. can all be interpreted as floor.

[0034] The above explanation does not completely include the relationship definitions given in this application, but only represents part of it.

[0035] The invention provides a multifunctional oscillating culture integrated shaker based on cell culture, which is used for oscillating enzyme biological cells. During the oscillation process, the maximum oscillation parameter is determined according to the oscillation reaction of liquid under different parameters.

[0036] See also Figure 1-3As shown, in one embodiment, the integrated shaking table includes a body 1 that can be placed on the ground and is equipped with an observation window, and a split oscillating platform 2 for placing a container is arranged inside the body 1, and the oscillating platform 2 is composed of a plurality of oscillating plates 21, and a clamping member 3 is arranged on the upper part of the oscillating plate 21 to clamp the container; The body 1 is internally configured with a driving module 4 connected to the oscillating platform 2. The driving module 4 includes an oscillating unit 5 and a connecting unit 6. The connecting unit 6 connects the oscillating unit 5 and the oscillating plate 21. The connecting unit 6 configures two transmission states of adjacent oscillating plates 21 through a differential. The differential is configured so that the driving module 4 drives multiple oscillating plates 21 to oscillate at the same speed or at a differential speed. The collecting device 8 is installed inside the body 1 to collect the liquid level change of the container during the oscillation process.

[0037] In one practicable manner, multiple oscillation plates 21 are connected by setting a connection unit 6 so that the multiple oscillation plates 21 can move synchronously, and the upper parts of the multiple oscillation plates 21 are each provided with a clamping member 3 to clamp the container to ensure the stability of the container during the oscillation process.

[0038] Optionally, the body 1 includes a control system for adjusting oscillation parameters, temperature, humidity and oscillation time, the control system is integrated inside the body 1, and a temperature control system is configured inside the body 1 to control the temperature change inside the body 1.

[0039] Among them, since the oscillation frequencies required by enzyme biological cells in different states are different, and due to the differences in the containers and the liquids inside them, the same oscillation frequency will have different effects. In order to avoid the problem that the shaker can only set one vibration frequency, the oscillation platform 2 is configured to be composed of multiple oscillation plates 21, and they are connected to each other through connecting units 6, so that differential oscillation can be performed between the oscillation plates 21.

[0040] See also Figure 1-3 As shown, in one embodiment, the body 1 further includes a retractable cover 22, a cover 23 for observation and sealing is hinged to the outside of the cover 22, and the cover 23 can be swung open; The cover body 22 can be retracted and installed inside the body 1, and a clamping plate 24 is arranged outside the body 1 to support and limit the cover body 22. When the cover body 22 is retracted, the volume of the rocking table is smaller than that when the cover body 22 is supported.

[0041] In one practicable manner, a retractable cover 22 is used to reduce the volume of the rocking bed when not in use, so as to facilitate carrying and transportation of the rocking bed. After the cover 22 is unfolded, the cover 22 is supported by a card plate 24 to ensure the stability of the cover 22.

[0042] Optionally, the card plate 24 is swingably arranged, one end of the card plate 24 is hinged to the body 1, and the other end is provided with a mouth, the cover body 22 extends outwardly corresponding to the position of the card plate 24 to form a protrusion, and the card plate 24 is perpendicular to the ground and the mouth is arranged in the direction to support the cover body 22.

[0043] Alternatively, the card plate 24 is swingably arranged, one end of the card plate 24 is installed on the outside of the body 1 through a rotating shaft and can rotate relative to the body 1, and a groove is arranged at the other end. The cover body 22 extends outwardly corresponding to the position of the card plate 24 to form a protrusion, and the card plate 24 supports the cover body 22 when the protrusion enters the groove.

[0044] Alternatively, the card plate 24 is independently provided, and a card slot capable of placing the card plate 24 is provided inside the machine body 1, one end of the card plate 24 is inserted into the card slot, and an opening or groove is provided at the other end, and the outer side of the cover body 22 protrudes from the opening or groove to cooperate with the opening or groove to ensure the stability of the cover body 22. Compared with the method of connecting the card plate 24 to the machine body 1, the method of independently providing the card plate 24 requires additional storage of the card plate 24, but reduces wear compared with the method of connecting the card plate 24 to the machine body 1.

[0045] See also Figure 4-6 As shown, in one embodiment, a first connecting member 61, a second connecting member 62, and a transmission member 63 connecting the first connecting member 61 and the second connecting member 62 and adjusting the transmission ratio of the first connecting member 61 and the second connecting member 62 are arranged inside the differential housing; The first connecting member 61 and the second connecting member 62 are respectively installed at the bottom of two adjacent oscillation plates 21 and are configured to realize the movement of the two adjacent oscillation plates 21 in a manner that the first connecting member 61 is used as an active member and the second connecting member 62 is used as a driven member.

[0046] In one practicable manner, the first connecting member 61 drives the transmission member 63 to change when it moves, and the transmission member 63 drives the second connecting member 62 to move according to the transmission ratio. Multiple oscillation plates 21 are connected through a differential. In a differential, the side close to the drive unit is the first connecting member 61.

[0047] Under the connection of the differential, adjacent oscillating plates 21 move in the same manner, namely: When the oscillating plate 21 connected to the first connecting member 61 is in linear reciprocating motion, the oscillating plate 21 connected to the second connecting member 62 is also in linear reciprocating motion; When the oscillating plate 21 connected to the first connecting member 61 moves in a circular motion, the oscillating plate 21 connected to the second connecting member also moves in a circular motion.

[0048] See also Figure 7-10 As shown, in one embodiment, the transmission member 63 includes: The first slider 631 and the second slider 632 are both disposed inside the differential and are connected to the first connecting member 61 and the second connecting member 62 respectively. The first connecting member 61 and the second connecting member 62 move synchronously with the first slider 631 and the second slider 632; The communication pipeline 634 is disposed inside the differential housing, and the first slider 631 and the second slider 632 slide to change the medium inside the communication pipeline 634; The pressure plate 635 is arranged inside the differential housing. A magnetic component 636 is configured at one end of the pressure plate 635 away from the first slider 631 or the second slider 632 to adjust the position of the pressure plate 635. The position change of the pressure plate 635 changes the cross-sectional area of ​​the slide groove 633 of the first slider 631 or the second slider 632. When the cross-sectional area of ​​the pressure plate 635 changes, the cross-sectional area of ​​the first slider 631 and the second slider 632 extending to the inner end of the slide groove 633 changes accordingly, and the connecting pipe 634 connects the slide groove 633.

[0049] In one practicable manner, the first slider 631 slides and changes its position to drive the medium disposed inside the slide groove 633 and the connecting pipe 634 to move, and the second slider 632 is moved by the movement of the medium. After the cross-sectional area of ​​the slide groove 633 is adjusted by the pressure plate 635, the first slider 631 and the second slider 632 are adjusted accordingly.

[0050] Exemplarily, the cross-sectional area of ​​the chute 633 where the second slider 632 is installed is reduced, and when the area of ​​the chute 633 of the first slider 631 remains unchanged, the second slider 632 and the first slider 631 move the same distance, and the medium required to drive the second slider 632 is smaller than the medium required for the first slider 631, thereby achieving differential motion between the first slider 631 and the second slider 632. The same speed or differential speed is achieved according to the change in the corresponding cross-sectional area of ​​the first slider 631 and the second slider 632.

[0051] The magnetic component 636 is an electromagnet.

[0052] See also Figure 11-12 As shown, in one embodiment, the first slider 631 and the second slider 632 are both provided with a folding plate 637 supported by a compression spring 638 and attached to the outer wall of the pressing plate 635. When the pressing plate 635 is squeezed, the folding plate 637 is also squeezed, and the pressing plate 635 and the folding plate 637 move synchronously. An inlet 639 and an outlet 6310 are arranged on both sides of the slide groove 633 where the first slider 631 and the second slider 632 are installed. The inlet 639 and the outlet 6310 on one side cannot be opened at the same time. A sealing member 6311 is arranged on the outer side of the slide groove 633 to move along the movement direction of the first slider 631 or the second slider 632. The sealing member 6311 extends to the slide groove 633 to seal the inlet 639 or the outlet 6310. The outlets 6310 or the inlet 639 on both sides of the slide groove 633 cannot be opened at the same time. The connecting pipe 634 connects the outlet 6310 of the slide groove 633 installed on the first slider 631 with the inlet 639 of the slide groove 633 installed on the second slider 632 , and vice versa.

[0053] In one feasible manner, in order to clarify the status of the outlet 6310 and the inlet 639, a one-way valve is arranged at one end of the connecting pipe 634 connecting the inlet 639 or the outlet 6310, that is, the inlet 639 can only perform the function of the inlet 639, and the outlet 6310 can only perform the function of the outlet 6310, thereby preventing the medium on the left side of the slide groove 633 from entering the right side through the connecting pipe 634 when the first slider 631 slides, and failing to drive the second slider 632 to move.

[0054] Since the driving speed of the first slider 631 is a fixed value, the speed at which the medium enters the second slider 632 is also a fixed value. When the cross-sectional area of ​​the slide groove 633 is adjusted, the differential speed of the second slider 632 is achieved.

[0055] One sealing member 6311 is installed corresponding to one chute 633. When the first slider 631 or the second slider 632 moves to one end of the chute 633, the sealing member 6311 is pushed to move. After the displacement, the sealing member 6311 seals the outlets 6310 or the inlet 639 on both sides of the chute 633. Exemplarily, when the first slider 631 is in the middle position and moves to the left, the outlet 6310 on the left side of the chute 633 is opened, and the inlet 639 on the right side of the chute 633 is opened. When the first slider 631 moves to the leftmost position, the sealing member 6311 is pushed to move. The sealing member 6311 seals the outlet 6310, so that the inlet 639 on the left side is opened and the outlet 6310 on the right side is opened. At this time, the first slider 631 starts to move to the right.

[0056] In another embodiment, the transmission member 63 forms different transmission ratios through the meshing of sliding gears to realize the transmission between the first connecting member 61 and the second connecting member 62. However, compared with the transmission method through the above transmission member 63, the structure of the differential will be larger, and the noise generated during the gear transmission process will be larger because the differential is gear-driven. The gear transmission method will increase the overall weight of the rocking bed.

[0057] See also Figure 8-10 As shown, in one embodiment, the first connecting member 61 and the second connecting member 62 both include: The connecting plate 611 is installed at the bottom of the oscillating plate 21 through a connecting structure. A connecting rod 612 is configured at one end of the connecting plate 611 to connect the first slider 631 and the connecting plate 611 or the second slider 632 and the connecting plate 611 .

[0058] In an practicable manner, the transmission member 63 is connected to the oscillation plate 21 through the first connection member 61 and the second connection member 62, so that the transmission member 63 and the oscillation plate 21 can move synchronously and ensure stability during the movement.

[0059] In one embodiment, the first connecting member 61 and the second connecting member 62 are configured as a four-bar mechanism, and the linear reciprocating motion of the first slider 631 or the second slider 632 is converted into a circular motion through the four-bar mechanism.

[0060] In an practicable manner, the movement state of the oscillation plate 21 can be adjusted by setting a four-bar mechanism, so that the oscillation plate 21 can generate different movement states.

[0061] See also Figure 14-15 As shown, in one embodiment, the clamping member 3 includes: A placement rack 31 is installed on the upper part of the oscillation plate 21. An arc-shaped soft board 32 is arranged inside the placement rack 31. Magnetic blocks 33 are arranged at both ends of the arc-shaped soft board 32. Adjacent magnetic blocks 33 are magnetically absorbed to shrink the cross-sectional area of ​​the arc-shaped soft board 32. The elastic body 34 is fixed to the side of the arc-shaped soft plate 32 that is in contact with the container, and the interior of the elastic body 34 is a cavity.

[0062] In one feasible method, the magnetic attraction of the magnetic block 33 can shrink the arc-shaped soft plate 32. When fixing the container, the arc-shaped soft plate 32 is shrunk so that the elastomer 34 on the inner side of the arc-shaped soft plate 32 fits the surface of the container. The elastomer 34 is set to be made of rubber material, and most of the container is made of glass. The rubber material increases the friction with the glass, thereby further improving the stability of the container during the oscillation process.

[0063] See also Figure 7 and 13 As shown, in one embodiment, the oscillation unit 5 includes: The electromagnet 51 is fixed inside the body 1, and a guide frame 52 is arranged on the outer wall of the oscillation plate 21 close to the electromagnet. A guide magnet 53 is arranged inside the guide frame 52, and the guide magnet 53 moves inside the guide frame 52; The positioning magnets 54 are fixed to the two ends of the inner wall of the guide frame 52 to support the guide magnet 53 and prevent the guide magnet 53 from contacting with the positioning magnet.

[0064] In one feasible method, the guide magnet 53 is controlled by an electromagnet. The electromagnets 51 are located on both sides of the guide frame 52. The guide magnet 53 is pushed by the magnetic force of the electromagnet 51 to generate inertia. The inertia is used to move the oscillation plate 21 in one direction. When the guide magnet 53 moves to the other end, the electromagnet 51 on the other side pushes the guide magnet 53 to move in the opposite direction.

[0065] The present application also provides an integrated shaking table oscillation method, which is used for the above-mentioned integrated shaking table, comprising the following steps: Determine the oscillation frequency of the enzyme biological cell to be oscillated, and place the container inside the clamp 3; The oscillation unit 5 drives one of the oscillation plates 21 to oscillate, and the oscillation plate 21 is connected to other oscillation plates 21 through the connection unit 6 to oscillate synchronously; The oscillation frequency of each oscillation plate 21 is adjusted by a differential; During the oscillation process, the acquisition device 8 acquires the image of the container liquid level during the oscillation process, and analyzes the container liquid level image to determine the fluctuation of the liquid level during the oscillation process.

[0066] Although the present invention is disclosed in conjunction with the above embodiments, it is not intended to limit the present invention. Anyone familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention should be based on the definition of the attached claims.

Claims

1. A multifunctional oscillating culture integrated shaker based on cell culture, comprising a body (1) that can be placed on the ground and is equipped with an observation window, characterized in that: The body (1) is internally provided with a split oscillating platform (2) for placing a container, the oscillating platform (2) being composed of a plurality of oscillating plates (21), and a clamping member (3) is disposed on the upper part of the oscillating plate (21) for clamping the container; The body (1) is internally provided with a driving module (4) connected to the oscillating platform (2); the driving module (4) comprises an oscillating unit (5) and a connecting unit (6); the connecting unit (6) connects the oscillating unit (5) and the oscillating plate (21); the connecting unit (6) configures two transmission states of adjacent oscillating plates (21) via a differential; the differential is configured so that the driving module (4) drives the plurality of oscillating plates (21) to oscillate at the same speed or at a differential speed; The collecting device (8) is installed inside the machine body (1) and collects the liquid level change of the container during the oscillation process.

2. A multifunctional oscillating culture integrated shaker based on cell culture according to claim 1, characterized in that: The machine body (1) further comprises a retractable cover body (22), a cover (23) for observation and sealing is hingedly connected to the outside of the cover body (22), and the cover (23) can be swung open; The cover body (22) is retractably mounted on the inner side of the machine body (1), and a clamping plate (24) is provided on the outer side of the machine body (1) for supporting and limiting the cover body (22); when the cover body (22) is retracted, the volume of the rocking table is smaller than when the cover body (22) is supported.

3. A multifunctional oscillating culture integrated shaker based on cell culture according to claim 2, characterized in that: A first connecting member (61), a second connecting member (62), and a transmission member (63) connecting the first connecting member (61) and the second connecting member (62) and adjusting the transmission ratio between the first connecting member (61) and the second connecting member (62) are arranged inside the differential housing; The first connecting member (61) and the second connecting member (62) are respectively mounted on the bottom of two adjacent oscillating plates (21) and are configured such that the first connecting member (61) acts as an active member and the second connecting member (62) acts as a driven member to achieve movement of the two adjacent oscillating plates (21).

4. The multifunctional oscillating culture integrated shaker based on cell culture according to claim 3, characterized in that: The transmission member (63) comprises: The first slider (631) and the second slider (632) are both arranged inside the differential and are respectively connected to the first connecting member (61) and the second connecting member (62); the first connecting member (61) and the second connecting member (62) move synchronously with the first slider (631) and the second slider (632); The communication pipeline (634) is arranged inside the differential housing, and the first slider (631) and the second slider (632) slide to change the medium inside the communication pipeline (634); A pressure plate (635) is arranged inside the differential housing. A magnetic component (636) is arranged at one end of the pressure plate (635) that is away from the first slider (631) or the second slider (632) to adjust the position of the pressure plate (635). The change in the position of the pressure plate (635) changes the cross-sectional area of ​​the slide groove (633) of the first slider (631) or the second slider (632). When the cross-sectional area of ​​the pressure plate (635) changes, the cross-sectional area of ​​the first slider (631) and the second slider (632) extending to the inner end of the slide groove (633) changes accordingly. The connecting pipe (634) is connected to the slide groove (633).

5. The multifunctional oscillating culture integrated shaker based on cell culture according to claim 4, characterized in that: The first slider (631) and the second slider (632) are both provided with a folding plate (637) supported by a compression spring (638) and in contact with the outer wall of the pressing plate (635); when the pressing plate (635) is compressed, the folding plate (637) is also compressed, and the pressing plate (635) and the folding plate (637) move synchronously; An inlet (639) and an outlet (6310) are arranged on both sides of the slide groove (633) on which the first slider (631) and the second slider (632) are installed. The inlet (639) and the outlet (6310) on one side cannot be opened at the same time. A sealing member (6311) is arranged on the outer side of the slide groove (633) to move along the movement direction of the first slider (631) or the second slider (632). The sealing member (6311) extends to the slide groove (633) to seal the inlet (639) or the outlet (6310). The outlets (6310) or the inlet (639) on both sides of the slide groove (633) cannot be opened at the same time. The connecting pipe (634) connects the outlet (6310) of the slide groove (633) installed on the first slider (631) and the inlet (639) of the slide groove (633) installed on the second slider (632), and connects the inlet (639) of the slide groove (633) installed on the first slider (631) and the outlet (6310) installed on the second slider (632).

6. The multifunctional oscillating culture integrated shaker based on cell culture according to claim 5, characterized in that: The first connecting member (61) and the second connecting member (62) both include: The connecting plate (611) is installed at the bottom of the oscillating plate (21) via a connecting structure, and a connecting rod (612) is arranged at one end of the connecting plate (611) to connect the first slider (631) and the connecting plate (611) or the second slider (632) and the connecting plate (611).

7. The multifunctional oscillating culture integrated shaker based on cell culture according to claim 5, characterized in that: The first connecting member (61) and the second connecting member (62) are configured as a four-bar mechanism, and the linear reciprocating motion of the first slider (631) or the second slider (632) is converted into circular motion through the four-bar mechanism.

8. The multifunctional oscillating culture integrated shaker based on cell culture according to claim 5, characterized in that: The clamping member (3) comprises: A placement rack (31) is installed on the upper part of the oscillation plate (21), wherein an arc-shaped soft plate (32) is arranged on the inner side of the placement rack (31), and magnetic blocks (33) are arranged at both ends of the arc-shaped soft plate (32), and the magnetic absorption between adjacent magnetic blocks (33) shrinks the cross-sectional area formed by the arc-shaped soft plate (32); The elastic body (34) is fixed to a side of the arc-shaped soft plate (32) that is in contact with the container, and the interior of the elastic body (34) is provided with a cavity.

9. The multifunctional oscillating culture integrated shaker based on cell culture according to claim 8, characterized in that: The oscillation unit (5) comprises: The electromagnet (51) is fixed inside the machine body (1), and a guide frame (52) is arranged on the outer wall of the oscillation plate (21) close to the electromagnet. A guide magnet (53) is arranged inside the guide frame (52), and the guide magnet (53) moves inside the guide frame (52); The positioning magnets (54) are fixed to the two ends of the inner wall of the guide frame (52) to support the guide magnet (53) and prevent the guide magnet (53) from contacting the positioning magnet.

10. An integrated shaking table oscillation method, used for the integrated shaking table according to any one of claims 1 to 9, characterized in that: The following steps are involved: Determine the oscillation frequency of the enzyme biological cell to be oscillated, and place the container inside the clamp (3); The oscillation unit (5) drives one of the oscillation plates (21) to oscillate, and the oscillation plate (21) is connected to the other oscillation plates (21) via the connection unit (6) to oscillate synchronously; adjusting the oscillation frequency of each oscillation plate (21) by means of a differential; During the oscillation process, the acquisition device (8) acquires the image of the container liquid level during the oscillation process, and analyzes the container liquid level image to determine the fluctuation of the liquid level during the oscillation process.

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