An organoid culture device and method for bladder cancer tumor cells

Abstract

The present invention belongs to the technical field of culturing organoids of bladder cancer tumor cells, and specifically relates to a culturing device and method for organoids of bladder cancer tumor cells, including a culture medium placement mechanism, a liquid drainage mechanism, a lifting drive mechanism, a culturing mechanism, and a sealing component. The culture medium placement mechanism is placed on a tabletop, and is used for placing the culture medium through the culture medium placement mechanism. The liquid drainage mechanism is rotatably connected to the lower end inside the culture medium placement mechanism, and the outer wall of the liquid drainage mechanism is connected to the drive end of the culture medium placement mechanism. The present invention can enable the new culture medium to interact and fuse with the culture medium inside the culturing mechanism, thereby reducing the operation steps of liquid replacement, lowering the difficulty of liquid replacement, and being able to achieve autonomous liquid drainage, making the taken-out bladder cancer tumor cells relatively clean and tidy.

Description

Technical Field

[0001] The present invention relates to the technical field of culturing bladder cancer tumor cell organoids, and specifically provides a device and method for culturing bladder cancer tumor cell organoids. Background Art

[0002] ‌A bladder cancer tumor cell organoid refers to a microscopic structure formed by bladder cancer tumor cells with similar organ structures and functions.

[0003] When culturing existing bladder cancer tumor cell organoids, it is necessary to culture the extracted and isolated bladder cancer tumor cells through a culture medium to provide necessary nutrients and environment for the bladder cancer tumor cells. The replacement of the culture medium needs to be carried out according to the cell growth state and experimental requirements, generally once every three days. If the color of the culture medium turns yellow, it needs to be replaced immediately. When replacing, a direct replacement method can be adopted to make the cells adapt to the new culture medium; a more gentle method can also be adopted. When passaging the cells, the new culture medium and the originally recommended culture medium are mixed in a certain proportion and gradually replaced, while closely observing the growth state of the cells to ensure that the cells can adapt to the new culture medium environment.

[0004] However, the existing devices for culturing bladder cancer tumor cell organoids are relatively simple. Generally, bladder cancer tumor cells and the culture medium are directly placed in glassware for culturing. According to the culture environment required by bladder cancer tumor cells, the glassware also needs to be placed in a carbon dioxide incubator for culturing. During the culturing process, the replacement of the culture medium requires manual operation by the operator. While gradually removing the culture medium to be replaced with a dropper or a pipette, new culture medium is injected into the glassware. Moreover, after the culture of the culture medium is completed, the liquid around the bladder cancer tumor cells also needs to be removed. Therefore, the culturing of bladder cancer tumor cell organoids is not only cumbersome in operation but also has too low efficiency.

[0005] Therefore, we propose a device and method for culturing bladder cancer tumor cell organoids. Summary of the Invention

[0006] To solve the above technical problems, the present invention provides the following technical solutions:

[0007] A device for culturing bladder cancer tumor cell organoids includes: a culture medium placement mechanism, a liquid discharge mechanism, a lifting drive mechanism, a culture mechanism, and a sealing component;

[0008] The culture medium placement mechanism is placed on the tabletop and is used to place the culture medium through the culture medium placement mechanism. The liquid drainage mechanism is rotatably connected to the lower end inside the culture medium placement mechanism. The outer wall of the liquid drainage mechanism is connected to the driving end of the culture medium placement mechanism and is driven by the culture medium placement mechanism to drive the liquid drainage mechanism to rotate, so that the liquid drainage mechanism drains the culture medium inside the culture medium placement mechanism. The lifting drive mechanism is installed at the center inside the culture medium placement mechanism. The bottom of the lifting drive mechanism is slidably connected to the bottom end inside the culture medium placement mechanism and is used to control the depth of the culture medium through the movement of the lifting drive mechanism. The culture mechanism is installed on the top of the lifting drive mechanism. The center of the bottom of the culture mechanism contacts the top of the lifting drive mechanism. At the same time, bladder cancer tumor cells are placed inside the culture mechanism, and the culture medium inside the culture medium placement mechanism is sucked and discharged into the culture mechanism through the drive of the lifting drive mechanism. The sealing component is detachably installed on the top of the culture medium placement mechanism and is used to seal the culture medium placement mechanism.

[0009] As a preferred scheme of a bladder cancer tumor cell organoid culture device according to the present invention, wherein: the culture medium placement mechanism includes: a guiding component, a placement component, and a liquid drainage driving component;

[0010] The guiding component is placed on the tabletop, and the top contacts the bottom of the lifting drive mechanism. The placement component is detachably installed on the top of the guiding component. The liquid drainage driving component is installed in the middle of the inner wall of the placement component.

[0011] As a preferred scheme of a bladder cancer tumor cell organoid culture device according to the present invention, wherein: the guiding component includes: a chassis and an arc groove;

[0012] The chassis is placed on the tabletop, and the arc groove is arranged around the surface of the chassis. The inside of the arc groove contacts the bottom of the lifting drive mechanism;

[0013] The placement component includes: a placement vessel, an annular partition, a liquid outlet groove, a first installation groove, a placement groove, a rotation groove, a first through groove, and a second through groove;

[0014] The placement vessel is detachably installed on the top of the chassis. The annular partition is arranged in the middle of the inner wall of the placement vessel. The liquid outlet groove is arranged around the bottom of the annular partition. A through hole is provided at the bottom of the liquid outlet groove. The first installation groove is arranged in the middle of the inner wall of the annular partition. The placement groove is arranged at the lower end of the inner wall of the placement vessel. The placement groove is arranged at the lower end of the annular partition. The rotation groove is arranged at the lower end of the inner wall of the placement vessel. The rotation groove is arranged at the lower end of the placement groove. The first through groove is arranged at the lower front side of the outer wall of the placement vessel. The second through groove is arranged in the middle of the left end of the outer wall of the placement vessel;

[0015] The liquid drainage driving component includes: a placement box, a rotation motor, and a gear;

[0016] The placement box is installed in the middle of the front end of the outer wall of the placement vessel. The rotation motor is arranged inside the placement box. The gear is connected to the output end of the rotation motor. The rear end of the outer wall of the gear penetrates through the first through groove. At the same time, the rear end of the outer wall of the gear is connected to the front end of the outer wall of the liquid discharge mechanism.

[0017] As a preferred solution of a bladder cancer tumor cell organoid culture device according to the present invention, wherein: the liquid discharge mechanism includes: a blocking component and a liquid discharge component;

[0018] The blocking component is rotatably connected inside the placement groove. The top of the blocking component contacts the bottom of the annular partition. The liquid discharge component is fixedly installed inside the placement groove. The top of the liquid discharge component is rotatably connected to the bottom of the blocking component.

[0019] As a preferred solution of a bladder cancer tumor cell organoid culture device according to the present invention, wherein: the blocking component includes: a first rotating disk, a second installation groove, a tooth groove, a return spring, a second rotating disk and a blocking plate;

[0020] The first rotating disk is rotatably connected inside the placement groove. The second installation groove is arranged on the outer side of the surface of the first rotating disk. The tooth groove is arranged around the outer wall of the first rotating disk. The tooth groove is meshed and connected with the gear. The return spring is arranged around the inside of the second installation groove. The second rotating disk is slidably connected inside the second installation groove. The bottom of the second rotating disk is connected to the top of the return spring. At the same time, the top of the second rotating disk contacts the bottom of the annular partition. The blocking plate is arranged around the top of the second rotating disk. The top of the blocking plate contacts the inside of the liquid outlet groove;

[0021] The liquid discharge component includes: a liquid discharge disk and a liquid discharge pipe;

[0022] The liquid discharge disk is fixedly installed at the lower end inside the placement groove. The top of the liquid discharge disk is rotatably connected to the bottom of the first rotating disk. The liquid discharge pipe is arranged at the left end of the outer wall of the liquid discharge disk. The liquid discharge pipe penetrates through the second through groove.

[0023] As a preferred solution of a bladder cancer tumor cell organoid culture device according to the present invention, wherein: the lifting drive mechanism includes: a limiting component and a lifting drive component;

[0024] The limiting component is rotatably connected inside the rotation groove. The outer wall of the lifting drive component is installed inside the first installation groove. The lower end of the lifting drive component is rotatably connected around the inside of the limiting component. The bottom of the lifting drive component is slidably connected inside the arc groove.

[0025] As a preferred solution of a bladder cancer tumor cell organoid culture device according to the present invention, wherein: the limiting component includes: a limiting disk, a third installation groove and a limiting rod;

[0026] The outer wall of the limit disk is provided with limit rods around it, the outer ports of the limit rods are slidably connected to the inside of the rotation groove, and the third installation groove is arranged around the surface of the limit disk;

[0027] The lifting drive assembly includes: a rubber sheet, a mounting plate, a hydraulic cylinder, a sealing box, a gripping rod, and a knob block;

[0028] The outer wall of the rubber sheet is mounted inside the first installation groove, the mounting plate is arranged at the central position of the rubber sheet, at the same time, the inner end of the rubber sheet is mounted around the outer wall of the mounting plate, the hydraulic cylinder is mounted at the top center of the mounting plate, the sealing box is mounted on the top of the mounting plate, at the same time, the sealing box hermetically wraps the hydraulic cylinder, the top of the gripping rod is rotatably connected to the periphery of the output end of the hydraulic cylinder, the two middle ends of the gripping rod are rotatably connected to the inside of the third installation groove, the bottom of the gripping rod is slidably connected to the inside of the arc groove, and the knob block is arranged around the top of the sealing box, and the knob block is connected to the bottom of the culture mechanism.

[0029] As a preferred solution of a bladder cancer tumor cell organoid culture device according to the present invention, wherein: the culture mechanism includes: a culture assembly and an auxiliary assembly;

[0030] The bottom of the culture assembly is detachably mounted on the top of the sealing box, the periphery of the bottom of the culture assembly is in contact with the top of the annular partition, at the same time, the inside of the culture assembly places bladder cancer tumor cells, and the auxiliary assembly is detachably mounted on the top of the culture assembly;

[0031] The culture assembly includes: a culture vessel, a liquid delivery pipeline, a chute, a piston, and a knob groove;

[0032] The culture vessel is placed inside the placing vessel, the inside of the culture vessel places bladder cancer tumor cells, the liquid delivery pipeline is arranged around the upper end of the outer wall of the culture vessel, the liquid delivery pipeline is communicated with the culture vessel, the bottom of the liquid delivery pipeline is in contact with the surface of the annular partition, the chute is arranged on the inner wall of the culture vessel, the piston is slidably connected to the inside of the chute, the knob groove is arranged around the bottom of the piston, and the knob block is detachably mounted inside the knob groove;

[0033] The auxiliary assembly includes: an auxiliary cover plate and a telescopic filter plate;

[0034] The auxiliary cover plate is detachably mounted on the top of the culture vessel, the top of the auxiliary cover plate is in contact with the bottom of the sealing assembly, the telescopic filter plate is arranged at the bottom center of the auxiliary cover plate, and the bottom of the telescopic filter plate is in contact with the surface of the piston.

[0035] As a preferred solution of a bladder cancer tumor cell organoid culture device according to the present invention, wherein: the sealing assembly includes: a sealing cover plate, an observation port, and a liquid injection valve;

[0036] The sealing cover plate is detachably installed on the top of the placement vessel. The observation port is arranged at the front end of the surface of the sealing cover plate. The liquid injection valve is arranged at the right end of the surface of the sealing cover plate.

[0037] A method for using a bladder cancer tumor cell organoid culture device includes the following operating methods:

[0038] S1: Place the culture vessel on the top of the sealing box. At this time, the knob groove at the bottom of the piston is inserted by the knob block. By rotating the culture vessel, the piston is driven to rotate, so that one end of the knob block is inserted into the groove on the inner side of the inner wall of the knob groove, thereby limiting the placement of the culture vessel.

[0039] S2: Put the extracted and separated bladder cancer tumor cells into the interior of the culture vessel, which is received by the surface of the piston. Then press and install the auxiliary cover plate on the top of the culture vessel. At this time, the bottom of the telescopic filter plate contacts the surface of the piston, so that the telescopic filter plate wraps and isolates the bladder cancer tumor cells, and connects the sealing cover plate to the placement vessel by pressing to seal the placement vessel.

[0040] S3: When culturing, inject the configured concentration of culture medium into the interior of the placement vessel through the liquid injection valve for storage. At this time, the blocking plate of the second rotating disk blocks the liquid outlet groove of the annular partition to prevent the culture medium from draining. By starting the hydraulic cylinder, the hydraulic cylinder drives the grasping rod to expand and contract, and under the limit of the arc groove on the surface of the chassis, the rubber sheet and the mounting plate are forced to perform an operation of rotating and lifting at the same time. Since the top of the auxiliary cover plate contacts the bottom of the sealing cover plate, the movement of the sealing box forces the piston to perform a rotary lifting movement, so that the culture medium in the placement vessel enters the interior of the culture vessel through the liquid delivery pipeline, soaks the bladder cancer tumor cells, and thus cultures the bladder cancer tumor cells.

[0041] S4: When observing the culture medium through the observation port after a period of time or when it is necessary to replace the culture medium, start the rotary motor, so that the rotary motor drives the gear and the first rotating disk to rotate, thereby driving the second rotating disk to rotate. Under the action of the reset spring, when the blocking plate rotates out of the liquid outlet groove, the second rotating disk sinks, so that the blocking plate smoothly disengages from the liquid outlet groove. At this time, the excess culture medium in the placement vessel falls into the interior of the drainage tray through the first rotating disk and the second rotating disk, and is discharged through the drainage pipeline. After draining until clean, make the blocking plate and the liquid outlet groove return to the initial position, and then inject the culture medium to be replaced into the interior of the placement vessel through the liquid injection valve. At this time, start the hydraulic cylinder again to make the new culture medium interact and mix with the culture medium in the culture vessel.

[0042] S5: After the cultivation is completed, the culture medium is drained through the drive of the hydraulic cylinder until it is clean. Then, the culture vessel is taken out, and subsequent operations are performed on the bladder cancer tumor cells in the culture vessel.

[0043] Compared with the prior art:

[0044] In the present invention, the bladder cancer tumor cells are stored separately through the setting of the culture mechanism, preventing the bladder cancer tumor cells from spreading to the entire vessel during cultivation due to being placed obliquely, thereby increasing the operation difficulty of liquid change.

[0045] Through the mutual cooperation of the liquid discharge mechanism, the lifting drive mechanism and the culture mechanism, the present invention can make the new culture medium interact and fuse with the culture medium inside the culture mechanism, thereby reducing the operation steps of liquid change, lowering the liquid change difficulty, and being able to achieve automatic liquid discharge, making the taken-out bladder cancer tumor cells relatively clean and tidy. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] Figure 1 It is the overall front view structural schematic diagram provided by the present invention;

[0047] Figure 2 It is the overall front view split structural schematic diagram provided by the present invention;

[0048] Figure 3 It is the internal structural schematic diagram of the culture medium placement mechanism provided by the present invention;

[0049] Figure 4 It is the split structural schematic diagram of the culture medium placement mechanism provided by the present invention;

[0050] Figure 5 It is the structural schematic diagram of the guiding component provided by the present invention;

[0051] Figure 6 It is the bottom view structural schematic diagram of the placement component provided by the present invention;

[0052] Figure 7 It is the split structural schematic diagram of the liquid discharge drive component provided by the present invention;

[0053] Figure 8 It is the split structural schematic diagram of the liquid discharge mechanism provided by the present invention;

[0054] Figure 9 It is the structural schematic diagram of the second installation groove provided by the present invention;

[0055] Figure 10 It is the connection structural schematic diagram of the lifting drive mechanism provided by the present invention;

[0056] Figure 11 It is the split structural schematic diagram of the lifting drive mechanism provided by the present invention;

[0057] Figure 12 Schematic structural diagram of the lifting drive assembly provided by the present invention;

[0058] Figure 13 Schematic split structure diagram of the grab bar and the limit assembly provided by the present invention;

[0059] Figure 14 Schematic split structure diagram of the culture mechanism provided by the present invention.

[0060] In the figure:

[0061] Culture medium placement mechanism 1, guiding component 11, chassis 111, arc groove 112, placement component 12, placement vessel 121, annular partition 122, liquid outlet groove 123, first installation groove 124, placement groove 125, rotation groove 126, first through groove 127, second through groove 128, liquid discharge drive assembly 13, placement box 131, rotation motor 132, gear 133, liquid discharge mechanism 2, blocking component 21, first rotating disk 211, second installation groove 212, tooth groove 213, return spring 214, second rotating disk 215, blocking plate 216, liquid discharge component 22, liquid discharge disk 221, liquid discharge pipeline 222, lifting drive mechanism 3, limit component 31, limit disk 311, third installation groove 312, limit rod 313, lifting drive assembly 32, rubber sheet 321, mounting plate 322, limit seat 323, hydraulic cylinder 324, sealing box 325, grab bar 326, culture mechanism 4, culture component 41, culture vessel 411, liquid transport pipeline 412, sliding groove 413, piston 414, knob groove 415, auxiliary component 42, auxiliary cover plate 421, telescopic filter plate 422, sealing component 5, sealing cover plate 51, observation port 52, liquid injection valve 53. Detailed implementation manners

[0062] To make the objectives, technical solutions, and advantages of the present invention clearer, the following will further describe the implementation manners of the present invention in detail with reference to the accompanying drawings.

[0063] The present invention provides a bladder cancer tumor cell organoid culture device and method. Please refer to Figures 1 - 14 , including a culture medium placement mechanism 1, a liquid discharge mechanism 2, a lifting drive mechanism 3, a culture mechanism 4, and a sealing component 5;

[0064] The culture medium placement mechanism 1 is placed on the tabletop and is used to place the culture medium through the culture medium placement mechanism 1. The culture medium placement mechanism 1 includes: a guiding component 11, a chassis 111, an arc groove 112, a placement component 12, a placement vessel 121, an annular partition 122, a liquid discharge groove 123, a first installation groove 124, a placement groove 125, a rotation groove 126, a first through groove 127, a second through groove 128, a liquid discharge driving component 13, a placement box 131, a rotation motor 132, and a gear 133. The guiding component 11 is placed on the tabletop, with its top in contact with the bottom of the lifting driving mechanism 3, and through the guiding component 11, the driving of the lifting driving mechanism 3 can be limited and guided. The chassis 111 is placed on the tabletop, and the arc groove 112 is arranged around the surface of the chassis 111. The inside of the arc groove 112 is in contact with the bottom of the lifting driving mechanism 3, and through the arc groove 112, the driving of the lifting driving mechanism 3 can be limited and guided. The placement component 12 is detachably installed on the top of the guiding component 11, and through the placement component 12, the liquid discharge mechanism 2 and the lifting driving mechanism 3 can be installed and placed. The placement vessel 121 is detachably installed on the top of the chassis 111. The annular partition 122 is arranged in the middle of the inner wall of the placement vessel 121, and through the annular partition 122, the culture medium can be stored. The liquid discharge groove 123 is arranged around the bottom of the annular partition 122. There are through holes at the bottom of the liquid discharge groove 123, and through the liquid discharge groove 123, the stored culture medium can be discharged. The first installation groove 124 is arranged in the middle of the inner wall of the annular partition 122, and through the first installation groove 124, the lifting driving mechanism 3 can be installed and placed. The placement groove 125 is arranged at the lower end of the inner wall of the placement vessel 121. The placement groove 125 is arranged at the lower end of the annular partition 122, and through the placement groove 125, the liquid discharge mechanism 2 can be limited and guided. The rotation groove 126 is arranged at the lower end of the inner wall of the placement vessel 121. The rotation groove 126 is arranged at the lower end of the placement groove 125, and through the rotation groove 126, the lower end components of the lifting driving mechanism 3 can be installed and limited. The first through groove 127 is arranged at the lower front side of the outer wall of the placement vessel 121. The second through groove 128 is arranged in the middle of the left end of the outer wall of the placement vessel 121. The liquid discharge driving component 13 is installed in the middle of the inner wall of the placement component 12, and through the cooperation of the placement component 12 and the liquid discharge driving component 13, the culture medium can be placed. The placement box 131 is installed in the middle of the front end of the outer wall of the placement vessel 121. The rotation motor 132 is arranged inside the placement box 131. The gear 133 is connected to the output end of the rotation motor 132. The rear end of the outer wall of the gear 133 passes through the first through groove 127. At the same time, the rear end of the outer wall of the gear 133 is connected to the front end of the outer wall of the liquid discharge mechanism 2, and through the rotation of the gear 133, the liquid discharge mechanism 2 can be driven to rotate.

[0065] The liquid drainage mechanism 2 is rotatably connected to the lower end inside the culture medium placement mechanism 1. The outer wall of the liquid drainage mechanism 2 is connected to the driving end of the culture medium placement mechanism 1, and is driven by the culture medium placement mechanism 1 to drive the liquid drainage mechanism 2 to rotate, so that the liquid drainage mechanism 2 drains the culture medium inside the culture medium placement mechanism 1. The liquid drainage mechanism 2 includes: a blocking component 21, a first rotating disk 211, a second installation groove 212, a tooth groove 213, a return spring 214, a second rotating disk 215, a blocking plate 216, a liquid drainage component 22, a liquid drainage disk 221 and a liquid drainage pipe 222. The blocking component 21 is rotatably connected inside the placement groove 125. The top of the blocking component 21 contacts the bottom of the annular partition 122, and the drainage of the annular partition 122 can be blocked by the blocking component 21. The first rotating disk 211 is rotatably connected inside the placement groove 125. The second installation groove 212 is arranged on the outer side of the surface of the first rotating disk 211. The tooth groove 213 is arranged around the outer wall of the first rotating disk 211. The tooth groove 213 is meshed and connected with the gear 133. The return spring 214 is arranged around the inside of the second installation groove 212. The second rotating disk 215 is slidably connected inside the second installation groove 212. The bottom of the second rotating disk 215 is connected to the top of the return spring 214. At the same time, the top of the second rotating disk 215 contacts the bottom of the annular partition 122. The blocking plate 216 is arranged around the top of the second rotating disk 215. The top of the blocking plate 216 contacts the inside of the liquid outlet groove 123, and the culture medium discharged from the liquid outlet groove 123 can be blocked by the blocking plate 216. The liquid drainage component 22 is fixedly installed inside the placement groove 125. The top of the liquid drainage component 22 is rotatably connected to the bottom of the blocking component 21, and the collected culture medium can be discharged through the liquid drainage component 22. The liquid drainage disk 221 is fixedly installed at the lower end inside the placement groove 125. The top of the liquid drainage disk 221 is rotatably connected to the bottom of the first rotating disk 211, and the culture medium discharged from the liquid outlet groove 123 can be collected through the liquid drainage disk 221. The liquid drainage pipe 222 is arranged at the left end of the outer wall of the liquid drainage disk 221. The liquid drainage pipe 222 penetrates through the second through groove 128, and the culture medium collected by the liquid drainage disk 221 can be discharged through the liquid drainage pipe 222.

[0066] The lifting drive mechanism 3 is installed at the inner center of the culture medium placement mechanism 1. The bottom of the lifting drive mechanism 3 is slidably connected to the inner bottom end of the culture medium placement mechanism 1, and the depth of the culture medium is controlled by the movement of the lifting drive mechanism 3. The lifting drive mechanism 3 includes: a limit component 31, a limit disk 311, a third installation groove 312, a limit rod 313, a lifting drive component 32, a rubber sheet 321, a mounting plate 322, a hydraulic cylinder 324, a sealing box 325, a gripping rod 326, and a knob block 327. The limit component 31 is rotatably connected to the inside of the rotation groove 126. The outer wall of the lifting drive component 32 is installed in the first installation groove 124. The outer circumference of the limit disk 311 is provided with limit rods 313. The outer ports of the limit rods 313 are slidably connected to the inside of the rotation groove 126. The third installation groove 312 is arranged around the surface of the limit disk 311. The lower end of the lifting drive component 32 is rotatably connected to the inner circumference of the limit component 31. The bottom of the lifting drive component 32 is slidably connected to the inside of the arc groove 112. The outer wall of the rubber sheet 321 is installed in the first installation groove 124. The mounting plate 322 is arranged at the center position of the rubber sheet 321. At the same time, the inner end of the rubber sheet 321 is installed around the outer wall of the mounting plate 322. The hydraulic cylinder 324 is installed at the top center of the mounting plate 322. The sealing box 325 is installed on the top of the mounting plate 322. At the same time, the sealing box 325 hermetically wraps the hydraulic cylinder 324. The top of the gripping rod 326 is rotatably connected to the periphery of the output end of the hydraulic cylinder 324. The middle two ends of the gripping rod 326 are rotatably connected to the inside of the third installation groove 312. The bottom of the gripping rod 326 is slidably connected to the inside of the arc groove 112. And through the drive of the hydraulic cylinder 324, the gripping rod 326 can be driven to expand and contract, so that the gripping rod 326 can drive the rubber sheet 321 and the mounting plate 322 to rotate and lift. The knob block 327 is arranged around the top of the sealing box 325. The knob block 327 is connected to the bottom of the culture mechanism 4, and the components at the bottom of the culture mechanism 4 can be driven through the knob block 327.

[0067] The culture mechanism 4 is installed on the top of the lifting drive mechanism 3. The center of the bottom of the culture mechanism 4 is in contact with the top of the lifting drive mechanism 3. At the same time, bladder cancer tumor cells are placed inside the culture mechanism 4, and the culture medium placed inside the culture medium placement mechanism 1 can be sucked and discharged into the culture mechanism 4 through the drive of the lifting drive mechanism 3. The culture mechanism 4 includes: a culture component 41, a culture vessel 411, a liquid delivery pipe 412, a chute 413, a piston 414, a knob groove 415, an auxiliary component 42, an auxiliary cover plate 421, and a telescopic filter plate 422. The bottom of the culture component 41 is detachably installed on the top of the sealed box 325. The periphery of the bottom of the culture component 41 is in contact with the top of the annular partition 122. At the same time, bladder cancer tumor cells are placed inside the culture component 4, and through the drive of the hydraulic cylinder 324, the internal components of the culture component 4 can be driven to perform lifting activities. The culture vessel 411 is placed inside the placement vessel 121, and bladder cancer tumor cells are placed inside the culture vessel 411. The liquid delivery pipe 412 is arranged around the upper end of the outer wall of the culture vessel 411. The liquid delivery pipe 412 is communicated with the culture vessel 411. The bottom of the liquid delivery pipe 412 is in contact with the surface of the annular partition 122. The chute 413 is arranged on the inner wall of the culture vessel 411. The piston 414 is slidably connected inside the chute 413, and the bladder cancer tumor cells can be pushed through the piston 414. The knob groove 415 is arranged around the bottom of the piston 414. The knob block 327 is detachably installed inside the knob groove 415, and through the cooperation of the knob groove 415 and the knob block 327, the placement of the culture vessel 411 can be limited. The auxiliary component 42 is detachably installed on the top of the culture component 4, and through the auxiliary component 42, the culture component 4 can be sealed. At the same time, the auxiliary component 42 can wrap and isolate the placed bladder cancer tumor cells. The auxiliary cover plate 421 is detachably installed on the top of the culture vessel 411. The top of the auxiliary cover plate 421 is in contact with the bottom of the sealing component 5. The telescopic filter plate 422 is arranged at the center of the bottom of the auxiliary cover plate 421. The bottom of the telescopic filter plate 422 is in contact with the surface of the piston 414, and through the telescopic filter plate 422, the placed bladder cancer tumor cells can be wrapped and isolated. At the same time, the telescopic filter plate 422 can be telescoped as the piston 414 moves up and down.

[0068] The sealing assembly 5 is detachably installed on the top of the culture medium placement mechanism 1, and the culture medium placement mechanism 1 is sealed by the sealing assembly 5. The sealing assembly 5 includes a sealing cover plate 51, an observation port 52, and a liquid injection valve 53. The sealing cover plate 51 is detachably installed on the top of the placement vessel 121, and the placement vessel 121 can be sealed and encapsulated through the sealing cover plate 51. The observation port 52 is arranged at the front end of the surface of the sealing cover plate 51, and the internal situation of the placement vessel 121 can be observed through the observation port 52. The liquid injection valve 53 is arranged at the right end of the surface of the sealing cover plate 51, and the culture medium can be injected into the interior of the placement vessel 121 through the liquid injection valve 53.

[0069] In specific use, a person skilled in the art places the culture vessel 411 on the top of the sealed box 325. At this time, the knob groove 415 at the bottom of the piston 414 is inserted by the knob block 327. By rotating the culture vessel 411, the piston 414 is driven to rotate, so that one end of the knob block 327 is inserted into the groove on the side of the inner wall of the knob groove 415, thereby limiting the placement of the culture vessel 411. The extracted and separated bladder cancer tumor cells are placed inside the culture vessel 411 and are received by the surface of the piston 414. Then, the auxiliary cover plate 421 is pressed and installed on the top of the culture vessel 411. At this time, the bottom of the telescopic filter plate 422 contacts the surface of the piston 414, so that the telescopic filter plate 422 wraps and isolates the bladder cancer tumor cells, and the sealed cover plate 51 is connected to the placement vessel 121 by pressing to seal the placement vessel 121. When culturing, the culture medium with a configured concentration is injected into the interior of the placement vessel 121 through the liquid injection valve 53 for storage. At this time, the baffle 216 of the second rotating disk 215 blocks the liquid outlet groove 123 of the annular partition 122 to prevent the culture medium from draining. By starting the hydraulic cylinder 324, the hydraulic cylinder 324 drives the grab bar 326 to expand and contract, and under the limitation of the arc groove 112 on the surface of the chassis 111, the rubber sheet 321 and the mounting plate 322 are forced to perform an operation of rotating and lifting at the same time. Since the top of the auxiliary cover plate 421 contacts the bottom of the sealed cover plate 51, the movement of the sealed box 325 forces the piston 414 to perform a rotary lifting movement, so that the culture medium inside the placement vessel 121 enters the interior of the culture vessel 411 through the liquid transport pipe 412 to soak the bladder cancer tumor cells, thereby culturing the bladder cancer tumor cells. At the same time, the liquid injection valve 53 can be externally connected to carbon dioxide or other gases required for culturing, so that the bladder cancer tumor cells are continuously cultured inside the culture vessel 411. When, after a period of time or by observing the culture medium through the observation port 52, it is necessary to replace the culture medium, by starting the rotary motor 132, the rotary motor 132 drives the gear 133 and the first rotating disk 211 to rotate, so that the second rotating disk 215 rotates. Under the action of the return spring 214, when the baffle 216 rotates out of the liquid outlet groove 123, the second rotating disk 215 sinks, so that the baffle 216 smoothly disengages from the liquid outlet groove 123. At this time, the excess culture medium inside the placement vessel 121 falls into the interior of the drainage tray 221 through the first rotating disk 211 and the second rotating disk 215 and is discharged through the drainage pipe 222. After being drained clean, the baffle 216 and the liquid outlet groove 123 are restored to their initial positions, and then the culture medium to be replaced is injected into the interior of the placement vessel 121 through the liquid injection valve 53. At this time, the hydraulic cylinder 324 is started again to make the new culture medium interact and mix with the culture medium inside the culture vessel 411, and the bladder cancer tumor cells are always inside the telescopic filter plate 422 under the isolation of the telescopic filter plate 422. After the culture is completed,The culture solution is drained until clean by the drive of the hydraulic cylinder 324, and then the culture vessel 411 is taken out, and subsequent operations are performed on the bladder cancer tumor cells in the culture vessel 411.

[0070] Although the present invention has been described above with reference to the embodiments, various modifications can be made thereto and components thereof can be replaced with equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the disclosed embodiments of the present invention can be combined with each other in any manner, and the exhaustive description of these combinations is not given in this specification only for the sake of saving space and resources. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A culturing device for bladder cancer tumor cell organoids, characterized in that: Comprising: a culture medium placement mechanism, a liquid drainage mechanism, a lifting drive mechanism, a culture mechanism, and a sealing assembly; The culture medium placement mechanism is placed on the tabletop, and is used for placing the culture medium through the culture medium placement mechanism. The liquid drainage mechanism is rotatably connected to the lower end inside the culture medium placement mechanism. The outer wall of the liquid drainage mechanism is connected to the driving end of the culture medium placement mechanism, and is driven by the culture medium placement mechanism to drive the liquid drainage mechanism to rotate, so that the liquid drainage mechanism drains the culture medium inside the culture medium placement mechanism. The lifting drive mechanism is installed at the center inside the culture medium placement mechanism. The bottom of the lifting drive mechanism is slidably connected to the bottom end inside the culture medium placement mechanism, and is used to control the depth of the culture medium through the movement of the lifting drive mechanism. The culture mechanism is installed on the top of the lifting drive mechanism. The center of the bottom of the culture mechanism contacts the top of the lifting drive mechanism, and is used to suck and discharge the culture medium inside the culture medium placement mechanism into the culture mechanism through the drive of the lifting drive mechanism. The sealing assembly is detachably installed on the top of the culture medium placement mechanism, and is used to seal the culture medium placement mechanism; The culture mechanism includes: a culture component and an auxiliary component; the culture component is placed inside the culture medium placement mechanism, and the auxiliary component is detachably installed on the top of the culture component; The culture component includes: a culture vessel, a liquid delivery pipeline, a chute, a piston, and a knob groove; bladder cancer tumor cells are placed inside the culture vessel; the liquid delivery pipeline is arranged around the upper end of the outer wall of the culture vessel, and the liquid delivery pipeline communicates with the culture vessel. The chute is arranged on the inner wall of the culture vessel, and the piston is slidably connected to the inside of the chute. The piston is forced to perform a rotary lifting movement through the movement of the lifting drive mechanism. The knob groove is arranged around the bottom of the piston; The auxiliary component includes: an auxiliary cover plate and a telescopic filter plate; the auxiliary cover plate is detachably installed on the top of the culture vessel, the top of the auxiliary cover plate contacts the bottom of the sealing assembly, and the telescopic filter plate is arranged at the center of the bottom of the auxiliary cover plate. The bottom of the telescopic filter plate contacts the surface of the piston.

2. The organoid culture device for bladder cancer tumor cells according to claim 1, wherein The culture medium placement mechanism includes: a guiding component, a placement component, and a liquid drainage drive component; The guiding component is placed on the tabletop, and the top contacts the bottom of the lifting drive mechanism. The placement component is detachably installed on the top of the guiding component, and the liquid drainage drive component is installed in the middle of the inner wall of the placement component.

3. The bladder cancer tumor cell organoid culture device according to claim 2, characterized in that, The guiding component includes: a chassis and an arc groove; The chassis is placed on the tabletop, and the arc groove is arranged around the surface of the chassis. The inside of the arc groove contacts the bottom of the lifting drive mechanism; The placement component includes: a placement vessel, an annular partition, a liquid outlet groove, a first installation groove, a placement groove, a rotation groove, a first through groove, and a second through groove; the periphery of the bottom of the culture component contacts the top of the annular partition; the bottom of the liquid delivery pipeline contacts the surface of the annular partition; The placing vessel is detachably installed on the top of the chassis. An annular partition is arranged in the middle of the inner wall of the placing vessel. A liquid outlet groove is arranged around the bottom of the annular partition. Through holes are arranged at the bottom of the liquid outlet groove. A first installation groove is arranged in the middle of the inner wall of the annular partition. A placing groove is arranged at the lower end of the inner wall of the placing vessel. The placing groove is arranged at the lower end of the annular partition. A rotating groove is arranged at the lower end of the inner wall of the placing vessel. The rotating groove is arranged at the lower end of the placing groove. A first through groove is arranged at the lower side of the front end of the outer wall of the placing vessel. A second through groove is arranged in the middle of the left end of the outer wall of the placing vessel; The liquid discharging driving assembly includes: a placing box, a rotating motor, and a gear; The placing box is installed in the middle of the front end of the outer wall of the placing vessel. The rotating motor is arranged inside the placing box. The gear is connected to the output end of the rotating motor. The rear end of the outer wall of the gear penetrates through the first through groove. At the same time, the rear end of the outer wall of the gear is connected to the front end of the outer wall of the liquid discharging mechanism.

4. A bladder cancer tumor cell organoid culture device according to claim 3, characterized in that The liquid discharging mechanism includes: a blocking assembly and a liquid discharging assembly; The blocking assembly is rotatably connected inside the placing groove. The top of the blocking assembly contacts the bottom of the annular partition. The liquid discharging assembly is fixedly installed inside the placing groove. The top of the liquid discharging assembly is rotatably connected to the bottom of the blocking assembly.

5. The organoid culture device for bladder cancer tumor cells according to claim 4, wherein The blocking assembly includes: a first rotating disk, a second installation groove, a tooth groove, a return spring, a second rotating disk, and a blocking plate; The first rotating disk is rotatably connected inside the placing groove. The second installation groove is arranged on the outer side of the surface of the first rotating disk. The tooth groove is arranged around the outer wall of the first rotating disk. The tooth groove is meshed and connected with the gear. The return spring is arranged around the inside of the second installation groove. The second rotating disk is slidably connected inside the second installation groove. The bottom of the second rotating disk is connected to the top of the return spring. At the same time, the top of the second rotating disk contacts the bottom of the annular partition. The blocking plate is arranged around the top of the second rotating disk. The top of the blocking plate contacts the inside of the liquid outlet groove; The liquid discharging assembly includes: a liquid discharging disk and a liquid discharging pipe; The liquid discharging disk is fixedly installed at the lower end inside the placing groove. The top of the liquid discharging disk is rotatably connected to the bottom of the first rotating disk. The liquid discharging pipe is arranged at the left end of the outer wall of the liquid discharging disk. The liquid discharging pipe penetrates through the second through groove.

6. The culture device for bladder cancer tumor cell organoids according to claim 5, wherein, The lifting driving mechanism includes: a limiting assembly and a lifting driving assembly; The limiting assembly is rotatably connected inside the rotating groove. The outer wall of the lifting driving assembly is installed inside the first installation groove. The lower end of the lifting driving assembly is rotatably connected to the inside around the limiting assembly. The bottom of the lifting driving assembly is slidably connected inside the arc groove.

7. The organoid culture device for bladder cancer tumor cells according to claim 6, characterized in that, The limiting assembly includes: a limiting disk, a third installation groove, and a limiting rod; Limiting rods are arranged around the outer wall of the limiting disk. The outer side ports of the limiting rods are slidably connected inside the rotating groove. The third installation groove is arranged around the surface of the limiting disk; The lifting driving assembly includes: a rubber sheet, a mounting plate, a hydraulic cylinder, a sealing box, a grasping rod, and a knob block; The bottom of the culture assembly is detachably installed on the top of the sealing box; The knob block is detachably installed inside the knob groove; The outer wall of the rubber sheet is installed inside the first installation groove. The mounting plate is arranged at the center position of the rubber sheet. At the same time, the inner end of the rubber sheet is installed around the outer wall of the mounting plate. The hydraulic cylinder is installed at the center of the top of the mounting plate, and the sealing box is installed at the top of the mounting plate. At the same time, the sealing box seals and wraps the hydraulic cylinder. The top of the gripper rod is rotatably connected around the output end of the hydraulic cylinder. The two middle ends of the gripper rod are rotatably connected inside the third installation groove. The bottom of the gripper rod is slidably connected inside the arc groove. The knob blocks are arranged around the top of the sealing box, and the knob blocks are connected to the bottom of the culture mechanism.

8. The organoid culture device for bladder cancer tumor cells according to claim 7, characterized in that, The sealing assembly includes: a sealing cover plate, an observation port and a liquid injection valve; The sealing cover plate is detachably installed on the top of the placement vessel. The observation port is arranged at the front end of the surface of the sealing cover plate. The liquid injection valve is arranged at the right end of the surface of the sealing cover plate.

9. A culture method using the bladder cancer tumor cell organoid culture device according to claim 8, characterized in that, It includes the following operation methods: S1: Place the culture vessel on the top of the sealing box. At this time, the knob groove at the bottom of the piston is inserted by the knob block. By rotating the culture vessel, the piston is driven to rotate, so that one end of the knob block is inserted into the groove on the inner wall side of the knob groove, thereby limiting the placement of the culture vessel. S2: Put the extracted and separated bladder cancer tumor cells into the culture vessel and place them on the surface of the piston. Then press and install the auxiliary cover plate on the top of the culture vessel. At this time, the bottom of the telescopic filter plate contacts the surface of the piston, so that the telescopic filter plate wraps and isolates the bladder cancer tumor cells, and connects the sealing cover plate to the placement vessel by pressing to seal the placement vessel. S3: When culturing, inject the configured concentration of culture medium into the placement vessel through the liquid injection valve for storage. At this time, the baffle of the second rotating disk blocks the liquid outlet groove of the annular partition to prevent the culture medium from draining. By starting the hydraulic cylinder, the hydraulic cylinder drives the gripper rod to expand and contract. Under the limit of the arc groove on the surface of the chassis, the rubber sheet and the mounting plate are forced to rotate and lift at the same time. Since the top of the auxiliary cover plate contacts the bottom of the sealing cover plate, the piston is forced to perform a rotary lifting movement through the movement of the sealing box, so that the culture medium in the placement vessel enters the culture vessel through the liquid delivery pipe, soaks the bladder cancer tumor cells, and thus cultures the bladder cancer tumor cells. S4: When it is necessary to replace the culture medium after a period of time or by observing the culture medium through the observation port, start the rotary motor, so that the rotary motor drives the gear and the first rotating disk to rotate, thereby driving the second rotating disk to rotate. Under the action of the return spring, when the baffle rotates out of the liquid outlet groove, the second rotating disk sinks, so that the baffle smoothly disengages from the liquid outlet groove. At this time, the excess culture medium in the placement vessel falls into the drainage tray through the first rotating disk and the second rotating disk and is discharged through the drainage pipe. After being drained clean, the baffle returns to the initial position with the liquid outlet groove, and then inject the culture medium to be replaced into the placement vessel through the liquid injection valve. At this time, start the hydraulic cylinder again to interact and mix the new culture medium with the culture medium in the culture vessel. S5: After the cultivation is completed, the culture solution is drained clean by the drive of the hydraulic cylinder, and then the culture vessel is taken out, and subsequent operations are performed on the bladder cancer tumor cells in the culture vessel.

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