A process for activating humic acid using enzyme preparations

Abstract

The present invention relates to the technical field of enzyme preparation applications, and particularly to a process for activating humic acid using enzyme preparations. The process includes obtaining mineral source raw materials, treating them to obtain a raw material mixture; inoculating EM bacterial agents into the raw material mixture at a preset inoculation amount and stirring to obtain a mixture to be fermented; promoting synchronous fermentation of bacteria and enzymes under preset fermentation conditions to form a fermentation product; adding enzyme preparations to the fermentation product and monitoring the gas emission amount in real time, and determining whether to perform fermentation node detection on the fermentation product according to the monitoring results; analyzing the fermentation efficiency, adjusting the preset inoculation amount based on the analysis results, and determining whether the fermentation process is over, thereby obtaining humic acid. The present invention accurately judges the fermentation end node according to the activity of the enzyme and the actual fermentation situation, effectively monitors the fermentation process and analyzes the fermentation efficiency, analyzes the factors causing low fermentation efficiency, and takes corresponding control measures to improve the fermentation efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of enzyme preparation applications, and particularly relates to a process for activating humic acid using enzyme preparations. Background Art

[0002] Humic acid is a type of high-molecular organic compound formed by the decomposition and transformation of animal and plant residues under the action of microorganisms, and is widely present in soil, water bodies, and sediments; it has a complex chemical structure and various functional groups, showing high biological activity, good adsorption properties, and slow-release effects; these characteristics of humic acid play an important role in improving soil fertility, promoting crop growth, and improving the quality of agricultural products. Due to the slow formation process of natural humic acid and its large structural heterogeneity, these factors limit its wide industrial application. Therefore, by applying various preparation methods of artificial humic acid, including microbial decomposition methods, catalytic / oxidation methods, and hydrothermal methods, etc., the aim is to improve the yield and quality of humic acid while reducing the environmental impact during the preparation process.

[0003] Chinese patent document with publication number CN109111321B discloses a method for activating humic acid and the activated humic acid, including: adding the humic acid to be activated, phosphoric acid, and ammonium nitrate phosphate into an activation reaction device, and activating the humic acid using phosphoric acid and ammonium nitrate phosphate to obtain activated humic acid and nitrogen oxides; it can be seen that in the existing methods for activating humic acid, there is a lack of effective monitoring of the fermentation process and analysis of the fermentation efficiency, resulting in low fermentation efficiency and thus low yield of humic acid. Summary of the Invention

[0004] Therefore, the present invention provides a process for activating humic acid using enzyme preparations to overcome the problems in the prior art, such as the lack of real-time monitoring of the fermentation process, inaccurate addition amount of enzyme preparations, low fermentation efficiency, and low yield of humic acid.

[0005] To achieve the above object, the present invention provides a process for activating humic acid using enzyme preparations, including,

[0006] Obtaining a mineral source raw material, and processing the mineral source raw material to obtain a raw material mixture;

[0007] Inoculating EM bacterial agent into the raw material mixture at a preset inoculation amount, and stirring to obtain a mixture to be fermented;

[0008] Putting the mixture to be fermented into a closed fermentation tank, and fermenting under preset fermentation conditions to form a fermentation product;

[0009] Adding an enzyme preparation to the fermentation product at a preset addition amount, and real-time monitoring the gas emission amount, and determining whether to perform fermentation node detection on the fermentation product according to the monitoring result;

[0010] Perform fermentation node detection on the fermentation product to analyze the fermentation efficiency, adjust the preset inoculation amount based on the analysis results, determine whether the fermentation process is completed by combining the real-time gray difference value, and then obtain humic acid;

[0011] Among them, the process of fermentation node detection is to take images of the side of the fermentation product to obtain real-time sedimentation images, draw an actual precipitation curve based on the real-time sedimentation images, and determine whether to control the valve to close and whether to supplement the enzyme preparation according to the actual precipitation curve.

[0012] Furthermore, the real-time monitoring of gas emissions and determining whether to perform fermentation node detection on the fermentation product according to the monitoring results include,

[0013] Obtain the gas emission amount discharged from the fermentation tank, and compare the standard emission amount with the gas emission amount:

[0014] If the gas emission amount is less than or equal to the standard emission amount, perform fermentation node detection on the fermentation product;

[0015] If the gas emission amount is greater than the standard emission amount, do not perform fermentation node detection on the fermentation product, and continue to monitor the gas emission amount for determination.

[0016] Furthermore, performing fermentation node detection on the fermentation product includes,

[0017] Take the initial fermentation image and continuous sedimentation images of the fermentation tank;

[0018] Calculate the initial gray value of the initial fermentation image and the average gray value of each continuous sedimentation image;

[0019] Calculate the ratio of the average gray value corresponding to any continuous sedimentation image to the initial gray value to obtain the real-time precipitation rate;

[0020] Determine whether the fermentation process is completed according to the real-time precipitation rate and the real-time gray difference value.

[0021] Furthermore, determining whether the fermentation process is completed according to the real-time precipitation rate and the real-time gray difference value includes,

[0022] Compare the standard precipitation rate with the real-time precipitation rate;

[0023] If the real-time precipitation rate is less than or equal to the standard precipitation rate, obtain the real-time gray difference value of the fermentation product, determine whether the fermentation process is completed based on the real-time gray difference value, and determine whether to supplement the enzyme preparation based on the sampling detection results;

[0024] If the real-time precipitation rate is greater than the standard precipitation rate, increase the preset stirring speed.

[0025] Further, determining whether the fermentation process is completed based on the real-time grayscale difference includes

[0026] Calculating the difference between the initial grayscale value and the average grayscale value corresponding to any continuous sedimentation image to obtain the real-time grayscale difference;

[0027] Comparing the standard grayscale difference with the real-time grayscale difference:

[0028] If the real-time grayscale difference is greater than the standard grayscale difference, draw the actual precipitation curve based on the continuous sedimentation images, and determine whether to control the valve to close according to the actual precipitation curve;

[0029] If the real-time grayscale difference is less than or equal to the standard grayscale difference, determine that the fermentation process is completed.

[0030] Further, determining whether to control the valve to close according to the actual precipitation curve includes

[0031] Obtaining the average grayscale value corresponding to each continuous sedimentation image;

[0032] Drawing a curve of the average grayscale value changing with time with a preset acquisition duration and performing linear fitting to obtain an average grayscale fitting curve;

[0033] Taking the maximum slope of the average grayscale fitting curve as the image grayscale change rate;

[0034] Obtaining the image grayscale change rate of each preset acquisition period, and drawing a curve of each image grayscale change rate changing with time as the actual precipitation curve;

[0035] Judging the change trend of the actual precipitation curve, and when a peak appears in the actual precipitation curve, controlling the valve to close and increasing the preset stirring speed.

[0036] Further, when no peak appears in the actual precipitation curve, do not control the valve to close, and determine whether to supplement the enzyme preparation based on the sampling detection result.

[0037] Further, determining whether to supplement the enzyme preparation based on the sampling detection result includes

[0038] Sampling and detecting the fermentation product to judge the enzyme activity:

[0039] If the enzyme activity is greater than the preset standard, increase the preset inoculation amount of EM bacteria agent;

[0040] If the enzyme activity is less than or equal to the preset standard, supplement the enzyme preparation to the fermentation product.

[0041] Furthermore, the enzyme preparation includes cellulase, ligninase or protease.

[0042] Furthermore, the treatment of the mineral source raw material includes

[0043] crushing and cleaning the mineral source raw material to obtain a raw material mixture;

[0044] adding water to the raw material mixture to make the water content of the raw material mixture reach the standard water content.

[0045] Compared with the prior art, the beneficial effects of the present invention are as follows: during the fermentation process, a pre-prepared enzyme preparation is added, and the preset addition amount is 0.5%-2% of the weight of the mineral source raw material, and the addition amount is controlled according to the enzyme activity. Regularly monitor the temperature, pH value and gas emission (such as carbon dioxide) during the fermentation process to ensure the stability of the reaction conditions, and record the changes during the fermentation process for subsequent analysis. According to the enzyme activity and the actual fermentation situation, accurately control the reaction time, and the reaction time is generally 7-14 days. By conducting sample detection, accurately judge the end point of fermentation, effectively monitor the fermentation process and analyze the fermentation efficiency, analyze the factors causing low fermentation efficiency, and take corresponding control measures to improve the fermentation effect.

[0046] Furthermore, by detecting the fermentation node of the fermentation product when it is determined that the gas emission in the fermentation tank is significantly reduced or even close to zero, accurately judge the fermentation situation, avoid the small gas emission caused by low fermentation efficiency, and ensure the effectiveness of judging whether the fermentation process is over.

[0047] Furthermore, analyze the color change of the fermentation product through image recognition technology, effectively monitor the fermentation process, analyze the color change and precipitation formation situation to analyze the fermentation efficiency. If it is determined that there is no peak in the actual precipitation curve, it means that the fermentation efficiency is not high, then determine the enzyme activity to determine whether this situation is caused by insufficient enzyme addition amount. If it is determined that there is a peak in the actual precipitation curve, indicating that the formed precipitate is stable, then increase the stirring speed, reduce the aggregation of the precipitate, maintain the fluidity of the mixture, prevent the precipitate from inhibiting the fermentation process, and make the microorganisms, nutrients and added enzyme preparation fully contact, thereby improving the fermentation efficiency.

[0048] Furthermore, when it is determined that the enzyme activity is greater than the preset standard, it means that the addition amount of the enzyme preparation is sufficient, and the reason for the low fermentation efficiency is that the preset inoculation amount is less, resulting in low microbial metabolic activity. Then increase the inoculation amount of the EM bacterial agent to improve the activity of the microorganisms, and thus improve the fermentation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0049] Figure 1 Schematic flow chart of the process for activating humic acid using enzyme preparation in an embodiment of the present invention;

[0050] Figure 2 Schematic flow chart of the process for treating mineral source raw materials in an embodiment of the present invention;

[0051] Figure 3 Schematic flow chart of the process for detecting fermentation nodes in an embodiment of the present invention;

[0052] Figure 4 Schematic flow chart of the process for determining whether to control the valve to close according to the actual precipitation curve in an embodiment of the present invention. Detailed implementation manners

[0053] In order to make the objectives and advantages of the present invention clearer, the present invention will be further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0054] The preferred implementation manners of the present invention will be described below with reference to the accompanying drawings. Those skilled in the art should understand that these implementation manners are only used to explain the technical principles of the present invention and do not limit the protection scope of the present invention.

[0055] It should be noted that in the description of the present invention, the terms indicating directions or positional relationships such as "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings. This is only for convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention.

[0056] In addition, it should be noted that in the description of the present invention, unless otherwise clearly defined and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0057] Please refer to Figure 1 and Figure 2 as shown Figure 1 Schematic flow chart of the process for activating humic acid using enzyme preparation in an embodiment of the present invention; Figure 2 Schematic flow chart of the process for treating mineral source raw materials in an embodiment of the present invention.

[0058] The present invention provides a process for activating humic acid using enzyme preparation, including,

[0059] Step S1, obtain ore source raw materials, and process the ore source raw materials to obtain a raw material mixture;

[0060] Step S101, pretreatment, crush and wash the ore source raw materials to obtain a raw material mixture;

[0061] Step S102, adjust moisture, add water to the raw material mixture to make the water content of the raw material mixture reach the standard water content;

[0062] Step S2, inoculate the EM bacterial agent into the raw material mixture at a preset inoculation amount and stir to obtain a mixture to be fermented;

[0063] Step S3, put the mixture to be fermented into a closed fermentation tank and ferment under preset fermentation conditions to form a fermentation product;

[0064] Step S4, enzymatic hydrolysis reaction, open the valve of the fermentation tank, add an enzyme preparation to the fermentation product at a preset addition amount, and monitor the gas emission amount in real time, and determine whether to perform fermentation node detection on the fermentation product according to the monitoring result;

[0065] Step S5, perform fermentation node detection on the fermentation product to analyze the fermentation efficiency, adjust the preset inoculation amount based on the analysis result, and combine the real-time grayscale difference value to determine whether the fermentation process is over, so as to obtain activated humic acid that meets the expectations;

[0066] Among them, the process of fermentation node detection is to take an image of the side of the fermentation product to obtain a real-time sedimentation image, draw an actual sedimentation curve based on the real-time sedimentation image, and determine whether to control the valve to close and whether to supplement and add an enzyme preparation according to the actual sedimentation curve.

[0067] In this embodiment, the mineral source raw material is weathered coal; during crushing and cleaning, a crusher is used to crush the weathered coal to an appropriate particle size (1-5 cm) to improve the contact efficiency between microorganisms and enzymes. By uniformly mixing different raw materials, the components are ensured to be evenly distributed; too low moisture content will affect the activity of microorganisms, while too high moisture content may lead to spoilage under anaerobic conditions. Therefore, the moisture content of the raw material is detected to ensure that the water content meets the standard, and the standard water content is between 50% and 60%. The moisture content can be adjusted by adding water or drying the raw material; the EM bacterial agent is an effective microbial bacterial agent, and the preset inoculation amount is 1%-5% of the weight of the mineral source raw material. Preferably, the preset inoculation amount is 1% of the weight of the mineral source raw material; gently stir to ensure the uniform distribution of the bacterial agent; the preset fermentation conditions include a preset fermentation temperature, a preset fermentation humidity, and a standard pH value. The preset fermentation temperature is between 30°C and 40°C, the preset fermentation humidity is between 50% and 60%, and the standard pH value is between 6.0 and 7.5 to promote the growth of microorganisms. Preferably, the preset fermentation temperature is 35°C, the preset fermentation humidity is 55%, and the standard pH value is 6.5; during the fermentation process, a pre-prepared enzyme preparation is added, and the preset addition amount is 0.5%-2% of the weight of the mineral source raw material. According to the activity of the enzyme, the addition amount is controlled, and the temperature, pH value, and gas emissions (such as carbon dioxide) during the fermentation process are regularly monitored to ensure the stability of the reaction conditions and record the changes during the fermentation process for subsequent analysis. According to the activity of the enzyme and the actual fermentation situation, the reaction time is accurately controlled. The reaction time is generally 7-14 days. By conducting sample tests, the fermentation end point is accurately judged, and then the generation situation of humic acid is evaluated in a timely manner. By using the enzyme preparation to activate humic acid, the yield and activity of humic acid are increased.

[0068] Specifically, the gas emission amount is monitored in real time. Determining whether to perform fermentation node detection on the fermentation product according to the monitoring result includes

[0069] Obtaining the gas emission amount discharged from the fermentation tank and comparing the standard emission amount with the gas emission amount:

[0070] If the gas emission amount is less than or equal to the standard emission amount, fermentation node detection is performed on the fermentation product in the fermentation tank;

[0071] If the gas emission amount is greater than the standard emission amount, fermentation node detection is not performed on the fermentation product in the fermentation tank, and the gas emission amount is continuously monitored for determination.

[0072] In this embodiment, the standard emission amount is the threshold for determining that no gas is generated, and the set value is close to zero. Since carbon dioxide and other gases will be generated by microorganisms during the fermentation process, if the gas emission amount is significantly reduced or even close to zero, it may indicate that the fermentation process may have been completed.

[0073] By detecting the fermentation node of the fermentation product when the gas emission in the fermentation tank is significantly reduced or even close to zero, the fermentation situation can be accurately determined, avoiding inaccurate monitoring results caused by low fermentation efficiency resulting in less gas emission, and ensuring the effectiveness of determining whether the fermentation process is over.

[0074] Refer to Figure 3 As shown, it is a schematic flow chart of detecting the fermentation node in an embodiment of the present invention;

[0075] Specifically, detecting the fermentation node of the fermentation product in the fermentation tank includes,

[0076] Step S401, taking an initial fermentation image and continuous sedimentation images of the side of the fermentation tank;

[0077] Step S402, calculating the initial gray value of the initial fermentation image and the average gray value of each continuous sedimentation image;

[0078] Step S403, calculating the ratio of the average gray value corresponding to any continuous sedimentation image to the initial gray value to obtain the real-time sedimentation rate;

[0079] Step S404, determining whether the fermentation process is over according to the real-time sedimentation rate and the real-time gray difference value.

[0080] Specifically, determining whether the fermentation process is over according to the real-time sedimentation rate and the real-time gray difference value includes,

[0081] Comparing the standard sedimentation rate with the real-time sedimentation rate:

[0082] If the real-time sedimentation rate is less than or equal to the standard sedimentation rate, obtain the real-time gray difference value of the fermentation product, determine whether the fermentation process is over based on the real-time gray difference value, and determine whether to supplement and add enzyme preparation based on the sampling detection result;

[0083] If the real-time sedimentation rate is greater than the standard sedimentation rate, increase the preset stirring speed;

[0084] Among them, increase the preset stirring speed to the corrected stirring speed, and the corrected stirring speed is the sum of the preset stirring speed and the adjustment step. The adjustment step is the sum of 1 and the degree of increase. The degree of increase is the product of the part where the real-time sedimentation rate exceeds the standard sedimentation rate and the real-time sedimentation rate.

[0085] As the fermentation process approaches completion, the color of the fermentation product darkens and precipitation occurs. Therefore, in this embodiment, the precipitation rate is characterized by calculating the percentage change in the color of the fermentation product relative to the initial color. The standard precipitation rate is set at 130%, indicating that when the percentage change in the fermentation product relative to the initial color reaches 130%, the color of the fermentation product has darkened and the fermentation process is progressing well. The initial gray value is 100. By analyzing the gray values of each pixel, the average gray value of the fermentation product is calculated. When it is determined that the real-time precipitation rate is greater than the standard precipitation rate, it means that the average gray value has reached or exceeded the set threshold value, which is 200. If the average gray value reaches or exceeds 200, it indicates that the fermentation process is good. For example, during the fermentation process, after a certain period of time, the monitored average gray value is 230. The real-time precipitation rate is calculated as [(230 - 100) / 100]×100% = 130%, indicating that the color of the fermentation product has darkened, the fermentation process is progressing well, and the fermentation is complete. The color change is monitored in real time to analyze the precipitation process, and the formation of precipitation during the fermentation process is detected in a timely manner. By monitoring the color change rate, the operating parameters are adjusted in a timely manner to optimize the fermentation process and improve the fermentation efficiency.

[0086] Specifically, determining whether the fermentation process is complete based on the real-time gray difference includes

[0087] calculating the difference between the initial gray value and the average gray value corresponding to any consecutive sedimentation images to obtain the real-time gray difference;

[0088] comparing the standard gray difference with the real-time gray difference:

[0089] If the real-time gray difference is greater than the standard gray difference, draw the actual precipitation curve according to the consecutive sedimentation images, and determine whether to control the valve to close according to the actual precipitation curve;

[0090] If the real-time gray difference is less than or equal to the standard gray difference, determine that the fermentation process is complete;

[0091] In this embodiment, the set standard gray difference is 230.

[0092] Refer to Figure 4 shown, which is a schematic flowchart of the present invention embodiment for determining whether to control the valve to close according to the actual precipitation curve;

[0093] Specifically, determining whether to control the valve to close according to the actual precipitation curve includes

[0094] Step S501, obtaining the average gray value corresponding to each consecutive sedimentation image;

[0095] Step S502: Draw a curve of the average gray value versus time with a preset acquisition duration, and perform linear fitting to obtain an average gray value fitting curve;

[0096] Step S503: Take the maximum slope of the average gray value fitting curve as the image gray value change rate;

[0097] Step S504: Obtain the image gray value change rate for each preset acquisition period, and draw a curve of each image gray value change rate versus time as the actual precipitation curve;

[0098] Step S505: Determine the change trend of the actual precipitation curve. When a peak appears in the actual precipitation curve, control the valve to close and increase the preset stirring speed;

[0099] When no peak appears in the actual precipitation curve, do not control the valve to close, and determine whether to supplement the enzyme preparation based on the sampling detection result;

[0100] Among them, increase the preset stirring speed to 1.1 times the preset stirring speed;

[0101] In this embodiment, the preset acquisition duration is set to 5 seconds; the preset acquisition interval is 1 second;

[0102] By analyzing the color change of the fermentation product through image recognition technology, effectively monitor the fermentation process, analyze the color change and precipitation generation situation to analyze the fermentation efficiency. If it is determined that no peak appears in the actual precipitation curve, it means the fermentation efficiency is not high. Then, determine the enzyme activity to determine whether this situation is caused by insufficient enzyme addition. If it is determined that a peak appears in the actual precipitation curve, it means the formed precipitate is stable. At this time, humic acid exists in the form of precipitation. Then, increase the stirring speed to reduce the aggregation of the precipitate, maintain the fluidity of the mixture, prevent the inhibition of the fermentation process by the precipitate, and enable the microorganisms, nutrients, and added enzyme preparation to fully contact, thereby improving the fermentation efficiency.

[0103] Specifically, determining whether to supplement the enzyme preparation based on the sampling detection result includes

[0104] Sampling and detecting the fermentation product to judge the enzyme activity:

[0105] If the enzyme activity is greater than the preset standard, increase the preset inoculation amount of EM bacteria agent to the corrected inoculation amount;

[0106] If the enzyme activity is less than or equal to the preset standard, supplement the enzyme preparation to the fermentation product;

[0107] Among them, the corrected inoculation amount is 1.5 times the preset inoculation amount; the supplementary addition of the enzyme preparation is 1.2 times the preset addition amount.

[0108] In this embodiment, colorimetry, fluorescence method or other biochemical detection methods are used to evaluate the activity of the enzyme. If the enzyme additive is cellulase, the preset standard is set to 10 U / mL. Since excessive addition of enzyme preparations may increase the viscosity of the mixture and affect the fluidity and fermentation efficiency of the mixture, by monitoring the enzyme activity and the formation of precipitation, unnecessary addition of enzyme preparations can be effectively avoided, the addition amount of enzyme preparations can be effectively controlled, and the efficient progress of the fermentation process can be ensured.

[0109] When it is determined that the enzyme activity is greater than the preset standard, it indicates that the addition amount of the enzyme preparation is sufficient. If the reason for the low fermentation efficiency is that the preset inoculation amount is small, resulting in low microbial metabolic activity, then by increasing the inoculation amount of EM bacteria agent, the activity of microorganisms can be improved, and thus the fermentation efficiency can be improved.

[0110] Example 1: The enzyme preparation is cellulase, and the raw material is 500 grams of weathered coal; the preset inoculation amount of EM bacteria agent is 10 grams (2%), the preset addition amount of cellulase is 15 g (3%), and the fermentation conditions are: temperature is 35 °C, pH value is 6.5, humidity is 55%, and the fermentation time is 8 days.

[0111] Example 2: The enzyme preparation is ligninase, and the raw material is 500 grams of weathered coal; the preset inoculation amount of EM bacteria agent is 10 grams (2%), the preset addition amount of ligninase is 15 g (3%), and the fermentation conditions are: temperature is 35 °C, pH value is 6.5, humidity is 55%, and the fermentation time is 9 days.

[0112] Example 3: The enzyme preparation is protease, and the raw material is 500 grams of weathered coal; the preset inoculation amount of EM bacteria agent is 10 grams (2%), the preset addition amount of protease is 15 g (3%), and the fermentation conditions are: temperature is 35 °C, pH value is 6.5, humidity is 55%, and the fermentation time is 10 days.

[0113] Table 1

[0114] ;

[0115] Table 1 shows the experimental results of activating humic acid with different enzyme preparations. From Table 1, it can be seen that different enzyme preparations have a significant impact on the formation of humic acid. Among them, the effect of cellulase is the best, providing experimental data that can confirm the successful preparation of humic acid with a higher content, proving the effectiveness of this process, and at the same time providing a control experiment.

[0116] Specifically, the humic acid samples and raw weathered coal samples obtained according to the above embodiments of the present invention are detected, and the humic acid content, free humic acid content, organic matter, and moisture are detected. The experimental data and experimental results are as follows: The experimental data are shown in Table 2. Table 2 is the detection and comparison results of the humic acid samples and raw weathered coal samples obtained in different batches of the above embodiments. Sample 1 is the raw weathered coal, and Sample 2 is the product of different batches corresponding to the cellulase enzyme preparation.

[0117] Table 2

[0118] ;

[0119] It can be known through detection that by using the preparation method of this embodiment, the free acid content in the humic acid (with weathered coal as the raw material) is increased to prepare a high-performance soil conditioner.

[0120] So far, the technical solution of the present invention has been described in combination with the preferred embodiments shown in the drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will all fall within the protection scope of the present invention.

[0121] The above are only the preferred embodiments of the present invention and are not used to limit the present invention; for those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent substitution, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A process for activating humic acid using enzyme preparations, characterized in that, including obtaining ore source raw materials, and processing the ore source raw materials to obtain a raw material mixture; inoculating an EM bacterial agent into the raw material mixture at a preset inoculation amount, and stirring to obtain a mixture to be fermented; putting the mixture to be fermented into a closed fermentation tank, and fermenting under preset fermentation conditions to form a fermentation product; adding an enzyme preparation to the fermentation product at a preset addition amount, and monitoring the gas emission amount in real time, and determining whether to perform a fermentation node detection on the fermentation product according to the monitoring result; performing a fermentation node detection on the fermentation product to analyze the fermentation efficiency, adjusting the preset inoculation amount based on the analysis result, and determining whether the fermentation process is ended in combination with the real-time gray difference value, so as to obtain humic acid; wherein, the process of the fermentation node detection is to take an image of the side of the fermentation product to obtain a real-time sedimentation image, draw an actual precipitation curve based on the real-time sedimentation image, and determine whether to control the valve to close and whether to supplement and add the enzyme preparation according to the actual precipitation curve; monitoring the gas emission amount in real time, and determining whether to perform a fermentation node detection on the fermentation product according to the monitoring result includes obtaining the gas emission amount discharged from the fermentation tank, and comparing the standard emission amount with the gas emission amount: if the gas emission amount is less than or equal to the standard emission amount, performing a fermentation node detection on the fermentation product; if the gas emission amount is greater than the standard emission amount, not performing a fermentation node detection on the fermentation product, and continuing to monitor the gas emission amount for determination; performing a fermentation node detection on the fermentation product includes taking an initial fermentation image and continuous sedimentation images of the fermentation tank; calculating the initial gray value of the initial fermentation image and the average gray value of each continuous sedimentation image; calculating the ratio of the average gray value corresponding to any continuous sedimentation image to the initial gray value to obtain a real-time precipitation rate; determining whether the fermentation process is ended according to the real-time precipitation rate and the real-time gray difference value.

2. The process for activating humic acid using an enzyme preparation according to claim 1, characterized in that, determining whether the fermentation process is ended according to the real-time precipitation rate and the real-time gray difference value includes comparing the standard precipitation rate with the real-time precipitation rate; if the real-time precipitation rate is less than or equal to the standard precipitation rate, obtaining the real-time gray difference value of the fermentation product, determining whether the fermentation process is ended based on the real-time gray difference value, and determining whether to supplement and add the enzyme preparation based on the sampling detection result; if the real-time precipitation rate is greater than the standard precipitation rate, increasing the preset stirring speed.

3. The process for activating humic acid using an enzyme preparation according to claim 2, characterized in that, determining whether the fermentation process is ended based on the real-time gray difference value includes calculating the difference between the initial gray value and the average gray value corresponding to any continuous sedimentation image to obtain a real-time gray difference value; comparing the standard gray difference value with the real-time gray difference value: if the real-time gray difference value is greater than the standard gray difference value, drawing an actual precipitation curve according to the continuous sedimentation image, and determining whether to control the valve to close according to the actual precipitation curve; if the real-time gray difference value is less than or equal to the standard gray difference value, determining that the fermentation process is ended.

4. The process for activating humic acid using an enzyme preparation according to claim 3, characterized in that, determining whether to control the valve to close according to the actual precipitation curve includes Obtain the average gray value corresponding to each consecutive sedimentation image; Draw a curve of the average gray value changing with time with a preset acquisition duration, and perform linear fitting to obtain an average gray fitting curve; Take the maximum slope of the average gray fitting curve as the image gray change rate; Obtain the image gray change rate of each preset acquisition period, and draw a curve of each image gray change rate changing with time as the actual sedimentation curve; Judge the change trend of the actual sedimentation curve. When a peak appears in the actual sedimentation curve, control the valve to close and increase the preset stirring speed.

5. The process for activating humic acid using an enzyme preparation according to claim 4, characterized in that, When no peak appears in the actual sedimentation curve, do not control the valve to close, and determine whether to supplement the enzyme preparation based on the sampling detection result.

6. The process for activating humic acid using an enzyme preparation according to claim 2, characterized in that, Determining whether to supplement the enzyme preparation based on the sampling detection result includes Sampling and detecting the fermentation product to judge the enzyme activity: If the enzyme activity is greater than the preset standard, increase the preset inoculation amount of the EM bacterium agent; If the enzyme activity is less than or equal to the preset standard, supplement the enzyme preparation to the fermentation product.

7. The process for activating humic acid by using enzyme preparation according to claim 1, wherein, The enzyme preparation includes cellulase, ligninase or protease.

8. The process for activating humic acid using an enzyme preparation according to claim 1, characterized in that, Processing the mineral source raw material includes Crushing and cleaning the mineral source raw material to obtain a raw material mixture; Adding water to the raw material mixture to make the water content of the raw material mixture reach the standard water content.

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