Flow cytometer

Abstract

The invention provides a flow cytometer, and relates to the technical field of flow cytometers, the flow cytometer comprises a main machine shell, a sample injector shell is fixedly arranged on the lower side of the right end of the main machine shell, the interior of the upper side of the right end of the main machine shell is communicated with the interior of the sample injector shell, and a sample injection assembly is arranged in the sample injector shell; a liquid path assembly and a power circuit assembly are arranged in the lower side of the main machine shell and distributed front and back, a stable optical platform assembly and a detection module assembly are arranged in the upper side of the main machine shell, and the stable optical platform assembly is electrically connected with the detection module assembly. The sampling assembly is arranged in the upper side of the right end of the main machine shell and corresponds to the sample injection assembly, the liquid path assembly is communicated with the sampling assembly and corresponds to the stable optical platform assembly, and the stable optical platform assembly has a stable anti-vibration effect. It can be guaranteed that a laser shaping light path in the stable optical platform assembly and a fluorescence splitting path received by the detection module are kept stable.

Description

Technical Field

[0001] The invention relates to the technical field of flow cytometers, in particular to a flow cytometer. Background Art

[0002] This flow cytometer is a high-end analytical instrument based on flow cytometry and spectral splitting technology. Its core feature is to analyze the fluorescence signals of cells or particles through full-spectrum detection technology. It can simultaneously capture the complete emission spectrum of fluorescent dyes, and combine algorithms to analyze overlapping spectra, significantly improving multi-color detection capabilities and data accuracy. It breaks through the limitation of "one detector for one marker" in traditional flow cytometry, and uses a series of optical elements (prisms, gratings, multiple filter combinations, etc.) to disperse the complete emission spectrum generated by the marker after excitation to a sensitive photodetector array to obtain the full spectrum signal of the cell; The optical elements in existing flow cytometers are easily disturbed by the vibration and temperature of the external environment during operation, making it difficult to maintain a stable light source path emitted by the optical elements. The spectral detectors in the optical elements have reduced operating efficiency at high temperatures, thus affecting the detection effect. Summary of the invention

[0003] The present invention provides a flow cytometer to solve the technical problem that the optical elements in the existing flow cytometers mentioned above are easily disturbed by the vibration and temperature of the external environment during operation, making it difficult to maintain the light source path emitted by the optical elements stable, and the spectral detector in the optical elements has reduced working efficiency at high temperatures, thereby affecting the detection effect.

[0004] In order to solve the above technical problems, the present invention discloses a flow cytometer, comprising a main machine housing, an injector housing is fixedly provided on the lower right side of the main machine housing, the interior of the upper right side of the main machine housing is connected with the interior of the injector housing, an injector assembly is provided inside the injector housing, a liquid path assembly and a power circuit assembly are provided inside the lower side of the main machine housing, the liquid path assembly and the power circuit assembly are distributed front to back, a stable optical platform assembly and a detection module assembly are provided inside the upper side of the main machine housing, the stable optical platform assembly and the detection module assembly are electrically connected, a sampling assembly is provided inside the upper right side of the main machine housing, the sampling assembly is correspondingly arranged with the injection assembly, the liquid path assembly is connected with the sampling assembly, the liquid path assembly and the stable optical platform assembly are correspondingly arranged, and a heat dissipation assembly is provided inside the main machine housing.

[0005] Preferably, the sampling component includes a sampling chamber arranged inside the upper right end of the main machine shell, a pulley 2 is respectively provided on the front and rear sides of the sampling chamber, the pulley 2 on the rear side is fixedly connected to the motor 2, the motor 2 is fixedly arranged in the sampling chamber, a conveyor belt 1 is connected between the pulleys on the front and rear sides, a mounting block is connected to the upper side of the conveyor belt 1, the mounting block is slidably arranged on the sliding block along the front and rear directions, the sliding block is fixedly arranged in the sampling chamber, pulleys 3 are respectively provided on the upper and lower sides of one end of the mounting block away from the conveyor belt 1, the conveyor belt 2 is connected between the pulleys 3 on the upper and lower sides, the front side of the conveyor belt 2 is fixedly connected to the mounting shell, the mounting shell is connected to the sampling needle, the upper pulley 3 is fixedly connected to the motor 3, and the motor 3 is fixedly arranged on the mounting block.

[0006] Preferably, a flip cover is provided at the upper end of the injector shell, and the injection assembly includes a cavity 1 arranged inside the injector shell, a bottom plate is fixedly provided on the lower side of the cavity 1, a motor 1 is fixedly provided on the upper end of the bottom plate, the motor 1 is connected to a pulley 1 on the right side, the pulley 1 on the right side is connected to a pulley 1 on the left side through a conveyor belt 3, the upper side of the conveyor belt 3 is connected to a positioning base shell, the positioning base shell is slidably arranged on the upper end of the bottom plate along the left and right directions, the upper end of the positioning base shell is used to place a sample tube rack, and the sample tube rack is correspondingly arranged on the lower side of the sampling needle.

[0007] Preferably, the liquid circuit assembly includes a liquid circuit cavity arranged inside the lower side of the main machine housing, and the interior of the liquid circuit cavity is respectively provided with peristaltic pump 1, peristaltic pump 2, diaphragm pump 1, diaphragm pump 2, filter 1, filter 2, a buffer tank, a plunger pump, a solenoid valve group and a three-way solenoid valve, and a liquid inlet pipe fitting and a liquid outlet pipe fitting are arranged at the lower left end of the main machine housing, the inlet of the liquid inlet pipe fitting is connected with the sheath liquid container through a liquid delivery pipe 1, the outlet of the liquid inlet pipe fitting is connected with the inlet of the diaphragm pump 1 through a liquid delivery pipe 2, the outlet of the diaphragm pump 1 is connected with the inlet of the solenoid valve group through a liquid delivery pipe 3, the outlet 1 of the solenoid valve group is connected with the inlet of the filter 1 through a connecting pipe 1, the outlet 2 of the solenoid valve group is connected with the inlet of the filter 2 through a connecting pipe 2, and the outlet of the filter 1 The outlet of the buffer tank and the outlet of the filter 2 are respectively connected with the inlet of the buffer tank through a liquid feeding tube 11, the outlet 1 of the buffer tank is connected with the inlet of the peristaltic pump 1 through a liquid feeding tube 4, the outlet of the peristaltic pump 1 is connected with a liquid feeding tube 5, the liquid feeding tube 5 is connected with the inlet of the plunger pump, the outlet of the plunger pump is connected with the port 1 of the three-way solenoid valve through a liquid feeding tube 6, the port 2 of the three-way solenoid valve is connected with the sampling needle through a liquid feeding tube 7, the port 3 of the three-way solenoid valve is connected with the inlet 1 of the flow chamber through a liquid feeding tube 8, the outlet 2 of the buffer tank is connected with the inlet of the peristaltic pump 2 through a liquid feeding tube 9, the outlet of the peristaltic pump 2 is connected with the inlet 2 of the flow chamber through a liquid feeding tube 10, and the outlet of the flow chamber is connected with the inlet 1 of the waste liquid container through a liquid feeding tube 12 and a liquid outlet pipe fitting.

[0008] Preferably, a collecting swab is provided in the sampling cavity, and the collecting swab is correspondingly arranged on the lower side of the sampling needle. The collecting swab is connected to the inlet of the diaphragm pump 2 through the liquid delivery tube 13, and the outlet of the diaphragm pump 2 is connected to the inlet 2 of the waste liquid container through the liquid delivery tube 14 and the liquid outlet pipe fitting.

[0009] Preferably, the stable optical platform assembly includes a cavity 2 arranged inside the upper side of the main machine housing, a partition is fixedly arranged between the cavity 2 and the sampling cavity, the partition is penetrated by a mounting hole for the liquid supply tube 10 to pass through, a stable shell is arranged on the right side of the upper end of the partition, three lasers are arranged inside the stable shell along the front-to-back direction, the three lasers and the three telephoto lenses are arranged one-to-one, the three telephoto lenses and the three dichroic mirrors are arranged one-to-one, the lasers, the telephoto lenses and the dichroic mirrors are arranged in sequence from left to right, a flow chamber is arranged correspondingly on the front side of the dichroic mirror, a rectifier block is arranged between the flow chamber and the frontmost dichroic mirror, the left side of the flow chamber is arranged correspondingly to the side scattering lens, the front side of the flow chamber is arranged correspondingly to the front scattering lens, the front scattering lens is connected correspondingly to the front scattering detector, the side scattering lenses are electrically connected to the detection module assemblies respectively, a vibration damping plate is arranged at the lower end of the stable shell, a buffer column is respectively arranged at the top corner of the lower end of the vibration damping plate, and the buffer column is fixedly connected to the partition.

[0010] Preferably, the heat dissipation assembly includes an air inlet arranged at the front end of the main machine shell, the air inlet is connected to the heat dissipation shell, an air outlet is arranged on the upper side of the rear end of the main machine shell, the air outlet is arranged corresponding to the heat dissipation shell, an exhaust fan is installed at the rear end of the heat dissipation shell, the heat dissipation shell is installed on the left side of the lower end of the partition, heat dissipation holes are penetrated at the front and rear ends of the heat dissipation shell, and a plurality of heat dissipation plates are evenly arranged in the heat dissipation holes along the left and right directions.

[0011] Preferably, the power circuit assembly includes a board box, and two exhaust fans are installed at the rear end of the board box. Two air outlets are provided on the lower side of the rear end of the main machine casing, and the two air outlets are connected to the two exhaust fans accordingly. A heat dissipation base plate is provided at the lower end of the board box, and an air inlet arranged at the left end of the main machine casing is connected to the lower interior of the casing.

[0012] Preferably, an exhaust fan three is installed at the rear end of the main machine housing, the board box and the exhaust fan three are distributed left and right inside the main machine housing, and an air outlet three is provided at the rear end of the main machine housing.

[0013] Compared with the prior art, the present invention has the following beneficial effects: The stable optical platform assembly has a stable anti-vibration effect. When external force is transmitted to the main machine housing, it can ensure that the emitted light source path in the stable optical platform assembly and the fluorescence spectroscopic path received by the detection module remain stable. The setting of the heat dissipation assembly is used to enable the spectral detector in the detection module assembly to operate within the target temperature range, thereby improving the working efficiency and operation stability of the spectral detector. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings: Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the top view of the structure of the present invention; Figure 3 It is a front view structural schematic diagram of the present invention; Figure 4 It is a schematic diagram of the internal structure of the present invention; Figure 5 It is a schematic diagram of the structure of the stable optical platform assembly of the present invention; Figure 6 This is a schematic diagram of the installation position of the board box of the present invention; Figure 7 It is a schematic diagram of the installation positions of the exhaust fan 1, the exhaust fan 2 and the exhaust fan 3 of the present invention; Figure 8 It is a schematic diagram of the board box structure of the present invention.

[0015] In the figure: 1, sampling chamber; 101, conveyor belt 1; 102, mounting block; 103, sliding block; 104, conveyor belt 2; 105, mounting shell; 106, sampling needle; 2, bottom plate; 201, motor 1; 202, conveyor belt 3; 203, positioning base shell; 3, liquid path chamber; 301, peristaltic pump 1; 302, diaphragm pump 1; 303, liquid inlet pipe; 304, filter 1; 305, buffer tank; 306, plunger pump; 307, solenoid valve group; 308, flow chamber; 309, liquid outlet pipe; 310, filter 2; 311, three-way solenoid valve; 312, peristaltic pump 2 ; 313, diaphragm pump two; 314, collecting swab; 315, liquid delivery tube ten; 4, stabilizing shell; 401, laser; 402, dichroic mirror; 403, rectifier block; 404, telephoto lens; 405, side scatter lens; 406, buffer column; 407, front scatter lens; 408, front scatter detector; 5, spectral detector; 6, heat dissipation shell; 7, main machine shell; 701, air inlet two; 8, sampler shell; 801, flip cover; 9, sample tube rack; 10, vibration damping plate; 11, board box; 12, heat dissipation base plate; 13, exhaust fan one; 14, exhaust fan two; 15, exhaust fan three. DETAILED DESCRIPTION

[0016] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0017] In addition, in the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes, and do not specifically refer to the order or sequence, nor are they used to limit the present invention. They are only used to distinguish components or operations described with the same technical terms, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions and technical features between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0018] The present invention provides the following embodiments.

[0019] Example 1

[0020] The embodiment of the present invention provides a flow cytometer, such as Figure 1-Figure 5 As shown, it includes a main machine housing 7, an injector housing 8 is fixedly provided on the lower right side of the main machine housing 7, the interior of the upper right side of the main machine housing 7 is connected with the interior of the injector housing 8, an injector assembly is provided inside the injector housing 8, a liquid path assembly and a power circuit assembly are provided inside the lower side of the main machine housing 7, the liquid path assembly and the power circuit assembly are distributed front to back, a stable optical platform assembly and a detection module assembly are provided inside the upper side of the main machine housing 7, the stable optical platform assembly and the detection module assembly are electrically connected, a sampling assembly is provided inside the upper right side of the main machine housing 7, the sampling assembly is arranged correspondingly to the injection assembly, the liquid path assembly is connected to the sampling assembly, the liquid path assembly and the stable optical platform assembly are arranged correspondingly, and a heat dissipation assembly is provided inside the main machine housing 7.

[0021] The beneficial effects of the above technical solution are: The sample to be detected can be placed on the injection component, and the sample to be detected is sent to the lower side of the right end of the main machine housing 7, so that the sample to be detected is on the lower side of the sampling component, and then the sample to be detected is sampled by the sampling component. The liquid path component is used to send the sample to be detected and the sheath liquid to the detection chamber for mixing. The sheath liquid can make the sample to be detected pass through the detection chamber along a straight line. The laser light source emitted by the stable optical platform component hits the sample to be detected, so that the sample to be detected generates a fluorescent signal. The detection module component converts the collected fluorescent signal into an electrical signal through a photomultiplier tube and amplifies the electrical signal for processing. The amplified electrical signal is sent to the computer system for data analysis to obtain spectral characteristics. The liquid path component is arranged inside the lower side of the main machine housing 7, and the stable optical platform component and the detection module component are arranged inside the upper side of the main machine housing 7. The liquid path component, the stable optical platform component, and the detection module component are separated to reduce the mutual influence between the liquid path and the optical path, and it is also convenient to maintain and replace the liquid path component, the stable optical platform component, and the detection module component separately. The component has a stabilizing and anti-vibration effect. When the external force is transmitted to the main machine housing 7, it can ensure that the light source path emitted by the stable optical platform component remains stable. The heat dissipation component is set to make the spectral detector 5 in the detection module component work within the target temperature range, thereby improving the working efficiency and operation stability of the spectral detector 5. It solves the problem that the optical elements in the existing flow cytometer are easily disturbed by the vibration and temperature of the external environment when working, making it difficult to keep the light source path emitted by the optical element stable, and the spectral detector in the optical element has a reduced working efficiency at high temperature, thereby affecting the stability of the detection effect. The sampling component, liquid path component, stable optical platform component, detection module component, sampling component and heat dissipation component can be installed in different areas of the main machine housing 7 as independent modules, so that the flow cytometer of the present invention is modularly designed, which is convenient for disassembly and assembly of the sampling component, liquid path component, stable optical platform component, detection module component, sampling component and heat dissipation component, thereby facilitating the manufacture and maintenance of the whole structure of the flow cytometer.

[0022] Example 2

[0023] On the basis of Example 1, Figure 1-Figure 5As shown, the sampling assembly includes a sampling chamber 1 arranged inside the upper right end of the main machine housing 7, a pulley 2 is respectively arranged on the front and rear sides of the sampling chamber 1, the pulley 2 on the rear side is fixedly connected to the motor 2, the motor 2 is fixedly arranged in the sampling chamber 1, a conveyor belt 101 is connected between the pulleys on the front and rear sides, a mounting block 102 is connected to the upper side of the conveyor belt 101, the mounting block 102 is slidably arranged on a sliding block 103 along the front and rear directions, the sliding block 103 is fixedly arranged in the sampling chamber 1, and pulleys 3 are respectively arranged on the upper and lower sides of one end of the mounting block 102 away from the conveyor belt 101, a conveyor belt 2 104 is connected between the pulleys 3 on the upper and lower sides, the front side of the conveyor belt 2 104 is fixedly connected to the mounting shell 105, the mounting shell 105 is connected to the sampling needle 106, the pulley 3 on the upper side is fixedly connected to the motor 3, and the motor 3 is fixedly arranged on the mounting block 102; A flip cover 801 is provided at the upper end of the sampler housing 8, and the sample injection assembly includes a cavity 1 arranged inside the sampler housing 8, a bottom plate 2 is fixedly provided at the lower side of the cavity 1, a motor 201 is fixedly provided at the upper end of the bottom plate 2, the motor 201 is connected to a pulley 1 on the right side, the pulley 1 on the right side is connected to a pulley 1 on the left side through a conveyor belt 3 202, the upper side of the conveyor belt 3 202 is connected to a positioning base shell 203, the positioning base shell 203 is slidably arranged on the upper end of the bottom plate 2 along the left and right directions, the upper end of the positioning base shell 203 is used to place a sample tube rack 9, and the sample tube rack 9 is correspondingly arranged on the lower side of the sampling needle 106.

[0024] The beneficial effects of the above technical solution are: When the motor 2 is working, it can drive the pulley 2 on the rear side to rotate, and the pulley 2 on the rear side drives the pulley 2 on the front side to rotate through the conveyor belt 101. When the conveyor belt 101 moves with the pulleys 2 on the front and rear sides, the mounting block 102 can move forward and backward with the conveyor belt 101. The mounting block 102 drives the pulleys 3 on the upper and lower sides, the motor 3, the conveyor belt 2 104, the mounting shell 105 and the sampling needle 106 to move forward and backward. When the motor 3 is working, it drives the pulley 3 on the upper side to rotate, and the pulley 3 on the upper side drives the pulley 3 on the lower side to rotate through the conveyor belt 2 104. When the conveyor belt 2 104 moves with the pulleys 3 on the upper and lower sides, the mounting shell 105 and the sampling needle 106 can move up and down, thereby achieving the purpose of moving the sampling needle 106 back and forth and up and down. When the motor 1 201 is working, it can drive the pulley 1 on the right to rotate, and the pulley 1 on the right drives the pulley 1 on the left to rotate through the conveyor belt 3 202. The conveyor belt 3 202 can move left and right as the pulleys 1 on the left and right sides rotate, thereby driving the positioning base shell 203 to move left and right, and the positioning base shell 203 drives the sample tube rack 9 to move left and right. When testing the sample, open the flip cover 801, place the sample tube rack 9 containing the sample on the positioning base shell 203, and then control the motor 1 201 to work, so that the sample tube rack 9 containing the sample moves to the corresponding position on the lower side of the sampling needle 106. Since the sampling needle 106 can move in the front and back, up and down directions, the sample tube rack 9 containing the sample can move left and right. Therefore, by controlling the motor 1 201, motor 2 and motor 3 to work, the sampling needle 106 can sample the sample at any position on the sample tube rack 9.

[0025] Example 3

[0026] On the basis of Example 2, Figure 1-Figure 5 As shown, the liquid circuit assembly includes a liquid circuit cavity 3 arranged inside the lower side of the main machine housing 7, and the liquid circuit cavity 3 is respectively provided with a peristaltic pump 1 301, a peristaltic pump 2 312, a diaphragm pump 1 302, a diaphragm pump 2 313, a filter 1 304, a filter 2 310, a buffer tank 305, a plunger pump 306, a solenoid valve group 307 and a three-way solenoid valve 311. A liquid inlet pipe 303 and a liquid outlet pipe 309 are provided at the left end of the lower side of the main machine housing 7. The inlet of the liquid inlet pipe 303 is connected with the sheath liquid container through a liquid feeding pipe 1, the outlet of the liquid inlet pipe 303 is connected with the inlet of the diaphragm pump 1 302 through a liquid feeding pipe 2, the outlet of the diaphragm pump 1 302 is connected with the inlet of the solenoid valve group 307 through a liquid feeding pipe 3, the outlet 1 of the solenoid valve group 307 is connected with the inlet of the filter 1 304 through a connecting pipe 1, the outlet 2 of the solenoid valve group 307 is connected with the inlet of the filter 2 310 through a connecting pipe 2, and the filter The outlet of the first filter 304 and the outlet of the second filter 310 are respectively connected to the inlet of the buffer tank 305 through a liquid delivery pipe 11, the outlet of the buffer tank 305 is connected to the inlet of the peristaltic pump 301 through a liquid delivery pipe 4, the outlet of the peristaltic pump 301 is connected to the liquid delivery pipe 5, the liquid delivery pipe 5 is connected to the inlet of the plunger pump 306, the outlet of the plunger pump 306 is connected to the port 1 of the three-way electromagnetic valve 311 through the liquid delivery pipe 6, and the port 1 of the three-way electromagnetic valve 311 is connected to the inlet of the plunger pump 306. Port 2 of the three-way solenoid valve 311 is connected to the sampling needle 106 through the liquid delivery tube 7, port 3 of the three-way solenoid valve 311 is connected to the inlet 1 of the flow chamber 308 through the liquid delivery tube 8, outlet 2 of the buffer tank 305 is connected to the inlet of the peristaltic pump 2 312 through the liquid delivery tube 9, the outlet of the peristaltic pump 2 312 is connected to the inlet 2 of the flow chamber 308 through the liquid delivery tube 10 315, and the outlet of the flow chamber 308 is connected to the inlet 1 of the waste liquid container through the liquid delivery tube 12 and the liquid outlet pipe 309; A collecting swab 314 is provided in the sampling chamber 1, and the collecting swab 314 is correspondingly arranged on the lower side of the sampling needle 106. The collecting swab 314 is connected to the inlet of the diaphragm pump 2 313 through the liquid delivery tube 13, and the outlet of the diaphragm pump 2 313 is connected to the inlet 2 of the waste liquid container through the liquid delivery tube 14 and the liquid outlet pipe 309.

[0027] The beneficial effects of the above technical solution are: The plurality of liquid delivery pipes and connecting pipes in the sheath liquid container and the waste liquid container liquid path assembly arranged outside the main machine housing 7 are not Figure 1-Figure 5 As shown, when the diaphragm pump 1 302 is working, the sheath liquid in the sheath liquid container is sent to the buffer tank 305 through the liquid inlet pipe 303, the liquid delivery pipe 1, the liquid delivery pipe 2, the liquid delivery pipe 3, the electromagnetic valve group 307, the connecting pipe 1, the filter 1 304 and the liquid delivery pipe 11. The filter 1 304 sterilizes the sheath liquid, and the buffer tank 305 temporarily stores the sheath liquid to ensure a stable supply of the sheath liquid. The sheath liquid in the buffer tank 305 enters the peristaltic pump 1 301 through the liquid delivery pipe 4 and enters the peristaltic pump 2 312 through the liquid delivery pipe 9. The peristaltic pump 2 312 sends the sheath liquid to the peristaltic pump 1 301. The liquid is directly sent into the flow chamber 308 to provide the sample with the sheath liquid. When the sample needs to be sampled, the peristaltic pump 1 301 and the peristaltic pump 2 312 do not work, the port 3 of the three-way solenoid valve 311 is disconnected, and the plunger pump 306 works to suck the sample out of the sampling needle 106, so that the sample enters the liquid delivery tube 6 through the liquid delivery tube 7, the port 2 and the port 1, and then the port 2 is closed and the port 3 is opened. Under the power of the peristaltic pump 1 301 and the plunger pump 306, the sample and the sheath liquid are sent into the flow chamber 308 through the liquid delivery tube 8 to mix with the sheath liquid in the flow chamber 308; During the sample detection process, under the power of peristaltic pump 2 312, peristaltic pump 1 301 and plunger pump 306, the sheath liquid and sample in the flow chamber 308 flow and enter the waste liquid container through the liquid delivery tube 12. When the sample detection is completed and the sampling needle 106 is cleaned, the port 3 of the three-way solenoid valve 311 is disconnected, the plunger pump 306 does not work, and the peristaltic pump 1 301 works to deliver the sheath liquid into the sampling needle 106 to rinse the sampling needle 106. The waste liquid after rinsing falls into the collection swab. The waste liquid in the collection swab enters the diaphragm pump 2 313 through the liquid delivery tube 9. The diaphragm pump 2 313 delivers the waste liquid into the waste liquid container through the liquid delivery tube 14. When the liquid path component is cleaned, the liquid delivery pipe 1 is connected to the cleaning liquid container so that the flow path of the cleaning liquid is the same as the flow path of the sheath liquid in the above process, and the liquid path component is cleaned. The settings of the filter 1 304 and the filter 2 310 are exactly the same. One of the filters is used as a spare. Specifically, if the filtering effect of the filter 1 304 is affected and needs maintenance and replacement, the solenoid valve group 307 is controlled, the inlet 1 of the solenoid valve group 307 is closed and the inlet 2 is opened, so that the sheath liquid flows from the filter 1 304 to the filter 2 310, and the outlet 1 of the solenoid valve group 307 is closed when the outlet 2 is opened, and the outlet 2 is closed when the outlet 1 is opened.

[0028] Example 4 On the basis of Example 3, Figure 1-Figure 6 As shown, the stable optical platform assembly includes a cavity 2 arranged inside the upper side of the main machine housing 7, a partition is fixedly arranged between the cavity 2 and the sampling cavity 1, and a mounting hole for the liquid supply pipe 10 315 is penetrated through the partition, a stable shell 4 is arranged on the right side of the upper end of the partition, and three lasers 401 are arranged inside the stable shell 4 along the front-to-back direction, the three lasers 401 and the three telephoto lenses 404 are arranged one-to-one, the three telephoto lenses 404 and the three dichroic mirrors 402 are arranged one-to-one, and the lasers 401, the telephoto lenses 404 and the dichroic mirrors 402 are arranged in sequence from left to right , a flow chamber 308 is provided corresponding to the front side of the dichroic mirror 402, a rectifying block 403 is provided between the flow chamber 308 and the frontmost dichroic mirror 402, a side scattering lens 405 is provided corresponding to the left side of the flow chamber 308, a front scattering lens 407 is provided corresponding to the front scattering lens 407, the front scattering lens 407 is connected to the front scattering detector 408, the side scattering lenses 405 are electrically connected to the detection module components, a vibration damping plate 10 is provided at the lower end of the stabilizing shell 4, a buffer column 406 is provided at the top corner of the lower end of the vibration damping plate 10, and the buffer column 406 is fixedly connected to the partition; The detection module assembly includes three spectrum detectors 5, which are electrically connected to the side diffuser lens 405 respectively, and the three spectrum detectors 5 are installed on the left side of the upper end of the partition; The heat dissipation assembly includes an air inlet 1 arranged at the front end of the main machine housing 7, the air inlet 1 is connected to the heat dissipation shell 6, an air outlet 1 is arranged on the upper side of the rear end of the main machine housing 7, the air outlet 1 is arranged corresponding to the heat dissipation shell 6, an exhaust fan 13 is installed at the rear end of the heat dissipation shell 6, the heat dissipation shell 6 is installed on the left side of the lower end of the partition, and heat dissipation holes are penetrated at the front and rear ends of the heat dissipation shell, and a number of heat dissipation plates are evenly arranged in the heat dissipation holes along the left and right directions.

[0029] The beneficial effects of the above technical solution are: The three lasers 401 are used to emit light of different wavelengths, the telephoto lens 404 is used to focus the light emitted by the corresponding laser 401, the dichroic mirror 402 is used to combine the light emitted by the corresponding laser 401, and the three combined light beams pass through the rectifier block 403, and the left and right sides of the rectifier block 403 are respectively provided with cylindrical lenses, so as to focus the three light beams, so that the focused light path hits the sample in the flow chamber 308, and the generated side scattered light is collected by the side scattered lens 405. The three spectral detectors 5 are of different models and are adapted to the three lasers 401 one by one, and are used to process the side scattered light generated by the light beam emitted by the laser 401 corresponding to the spectral detector 5 hitting the sample. The side scattered light can be used to detect the internal structural properties of cells. The forward scattered light generated by hitting the sample enters the forward scattered detector through the forward scattered lens 407. The forward scattered light is used as a threshold to exclude debris in the sample and small particles in the sheath liquid to avoid interference with the cells in the sample being tested. The setting of the buffer column 406 makes the stable shell 4 have a shock-absorbing and buffering effect. When the external force is transmitted to the main machine housing 7, the lasers and laser paths emitted by the three lasers 401 remain stable, avoiding the laser path from being offset and unable to hit the sample. It can ensure that the light source path emitted by the stable optical platform component remains stable, thereby making the collection of optical signals stable and improving the detection effect. The spectral detector 5 collects signal data of the side scattered light and analyzes it to obtain a fluorescence signal, the forward scattered light detector collects signal data of the forward scattered light and analyzes it to obtain a fluorescence intensity threshold range, the processed fluorescence intensity is compared with the fluorescence intensity threshold range, the fluorescence intensity exceeding the threshold range is removed, until the remaining fluorescence intensities that are not filtered out meet the fluorescence intensity threshold range, the remaining fluorescence signals are used as an array, the obtained fluorescence signal array is converted into an electrical signal by a photomultiplier tube and the electrical signal is amplified and processed, the amplified electrical signal is sent to a computer system for data analysis to obtain spectral characteristics (the three lasers 401, the photomultiplier tube, the forward scattered light detector and the spectral detector 5 are all existing products, and the detector collects signal data of the light and analyzes it to obtain the fluorescence intensity, the photomultiplier tube is used to convert the collected fluorescence signal into an electrical signal and the electrical signal is amplified and processed, and the amplified electrical signal is sent to a computer system for data analysis. The existing technology is used, and the present invention will not repeat it); The setting of the air inlet 1 allows the outside air to enter the interior of the main machine housing 7, pass through the heat dissipation shell 6 and then be discharged through the air outlet 1. A number of heat dissipation plates are evenly arranged in the heat dissipation holes along the left and right directions, and a protective fence is also provided at the front end of the heat dissipation holes. Adjacent heat dissipation plates constitute a heat dissipation channel, so that the air passes through the number of heat dissipation channels and finally flows out through the air outlet. The contact end between the partition and the three spectrum detectors 5 is fixedly connected to the upper end of the heat dissipation shell 6. The contact end between the partition and the three spectrum detectors 5 (the partition and the PCB circuit board of the spectrum detector 5 are in direct contact) and the heat dissipation shell 6 are made of the same material, which is a heat conductive material, thereby improving the heat dissipation effect of the three spectrum detectors 5. Optionally, the exhaust fan 13 in the air outlet can be automatically controlled by the following control method, specifically, a temperature sensor 1 is set at an air inlet to detect the temperature of the air flowing in the air inlet 1; a plurality of temperature sensors 2 are installed in the heat dissipation shell 6, and the plurality of temperature sensors 2 are respectively arranged between the heat dissipation channels formed by adjacent heat dissipation plates, so as to detect the temperature in different heat dissipation channels; a plurality of wind speed sensors are installed in the heat dissipation shell 6, and the plurality of wind speed sensors are respectively arranged between the heat dissipation channels formed by adjacent heat dissipation plates, so as to detect the wind speed in different heat dissipation channels; a controller is set, and the controller is electrically connected to the temperature sensor 1, the plurality of temperature sensors 2, the plurality of wind speed sensors and the frequency converter, and the frequency converter is electrically connected to the exhaust fan, and the controller calculates the theoretical wind speed required for the heat dissipation channel with the highest temperature per unit time to reach the target temperature according to the detection value of the temperature sensor 1, the detection values ​​of the plurality of temperature sensors 2 and formula (1); ; (1) The theoretical wind speed required for the heat dissipation channel with the highest temperature per unit time to reach the target temperature. is the maximum detection value among several temperature sensors, is the detection value of temperature sensor 1, is the air density, is the dynamic viscosity of the air in the heat dissipation channel, is the gap between adjacent heat sinks, is the Prandtl number, which takes a value of 0.5. is the thickness of the partition, is the thermal conductivity of the partition, is the thickness of the upper end of the heat dissipation shell 6, is the thermal conductivity of the heat dissipation shell 6, is the target temperature of the heat dissipation channel, is the thermal conductivity of air; Formula (1) is obtained from Converted, if the temperature value of the heat dissipation channel with the highest temperature is greater than the target temperature, the controller automatically increases the speed of the exhaust fan through the frequency converter to increase the wind speed in the heat dissipation channel, and the controller compares the minimum wind speed in the heat dissipation duct detected by several wind speed sensors and the theoretical wind speed required for the heat dissipation channel with the highest temperature per unit time calculated above to reach the target temperature, until the minimum wind speed in the heat dissipation duct detected by several wind speed sensors reaches the theoretical wind speed required for the heat dissipation channel with the highest temperature per unit time calculated above to reach the target temperature. At this time, the controller no longer adjusts the speed of the exhaust fan through the frequency converter, so that the speed of the exhaust fan maintains the current speed, thereby ensuring the heat dissipation effect of the three spectrum detectors 5, so that the spectrum detector 5 can operate stably, and the unit time is 1s. , so that the temperature of the heat dissipation duct can be adjusted in time to avoid the cooling time of the heat dissipation duct being too long, which affects the operation of the spectrum detector 5. If the temperature value of the heat dissipation channel with the highest temperature is lower than the target temperature, that is, when the theoretical wind speed required for the heat dissipation channel with the highest temperature per unit time calculated above to reach the target temperature is a negative value, the controller reduces the speed of the exhaust fan through the frequency converter to make it work in a low energy consumption state until the temperature value of the heat dissipation channel with the highest temperature is greater than the target temperature. Repeat the above-mentioned controller automatically increases the speed of the exhaust fan through the frequency converter to increase the wind speed in the heat dissipation channel. By automatically adjusting the speed of the exhaust fan, the spectrum detector 5 is automatically cooled, ensuring that the spectrum detector 5 can always operate stably without manual operation, saving time and effort.

[0030] Example 5

[0031] On the basis of Example 3, Figure 6-Figure 8 As shown, the power circuit assembly includes a board box 11, in which electrical parts are installed, an exhaust fan 14 is installed at the rear end of the board box 11, an air outlet 2 is provided at the lower side of the rear end of the main machine housing 7, and the air outlet 2 is correspondingly connected to the exhaust fan 14, a heat dissipation bottom plate 12 is provided at the lower end of the board box 11, and an air inlet 701 provided at the left end of the main machine housing 7 is connected to the lower side of the main machine housing 7; An exhaust fan 3 15 is installed at the rear end of the main machine housing 7. The board box 11 and the exhaust fan 3 15 are distributed left and right inside the main machine housing 7. An air outlet 3 is provided at the rear end of the main machine housing 7.

[0032] The beneficial effects of the above technical solution are: The board box 11 and the electrical components installed therein are equivalent to the existing electric control cabinet, and the present invention will not be described in detail. When the exhaust fan 14 is working, the outside air will enter the interior of the main machine housing 7 through the air inlet 2 701, and then be discharged through the air outlet 2, which can dissipate heat for the electrical components in the board box 11. When the exhaust fan 15 is working, the outside air will enter the interior of the main machine housing 7 through the air inlet 2 701, and then be discharged through the air outlet 3, which can dissipate heat for the interior of the main machine housing 7, such as the liquid path components, thereby improving the working stability of the flow cytometer of the present invention. Filters can be set at the air inlet 1 and the air inlet 2 701 to prevent dust carried by the air from affecting the components in the flow cytometer.

[0033] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A flow cytometer, characterized in that: The invention comprises a main machine housing (7), wherein a sample injector housing (8) is fixedly arranged on the lower right side of the main machine housing (7), the interior of the upper right side of the main machine housing (7) is communicated with the interior of the sample injector housing (8), a sample injector assembly is arranged inside the sample injector housing (8), a liquid path assembly and a power circuit assembly are arranged inside the lower side of the main machine housing (7), the liquid path assembly and the power circuit assembly are arranged front to back, a stable optical platform assembly and a detection module assembly are arranged inside the upper side of the main machine housing (7), the stable optical platform assembly and the detection module assembly are electrically connected, a sampling assembly is arranged inside the upper right side of the main machine housing (7), the sampling assembly is arranged correspondingly to the sample injector assembly, the liquid path assembly is communicated with the sampling assembly, the liquid path assembly and the stable optical platform assembly are arranged correspondingly, and a heat dissipation assembly is arranged inside the main machine housing (7).

2. A flow cytometer according to claim 1, characterized in that: The sampling assembly comprises a sampling chamber (1) arranged inside the upper right end of a main machine housing (7), a belt pulley 2 being respectively arranged on the front and rear sides of the sampling chamber (1), the belt pulley 2 on the rear side being fixedly connected to the motor 2, the motor 2 being fixedly arranged in the sampling chamber (1), a conveyor belt 1 (101) being connected between the belt pulleys on the front and rear sides, a mounting block (102) being connected to the upper side of the conveyor belt 1 (101), the mounting block (102) being slidably arranged on a sliding block (103) along the front-to-back direction, the sliding block (103) being fixedly arranged in the sampling chamber (1), a belt pulley 3 being respectively arranged on the upper and lower sides of an end of the mounting block (102) away from the conveyor belt 1 (101), a conveyor belt 2 (104) being connected between the belt pulleys on the upper and lower sides, the front side of the conveyor belt 2 (104) being fixedly connected to the mounting shell (105), the mounting shell (105) being connected to the sampling needle (106), the belt pulley 3 on the upper side being fixedly connected to the motor 3, the motor 3 being fixedly arranged on the mounting block (102).

3. A flow cytometer according to claim 2, characterized in that: A flip cover (801) is provided at the upper end of the sampler housing (8), and the sampler assembly comprises a cavity 1 arranged inside the sampler housing (8), a bottom plate (2) is fixedly provided at the lower side of the cavity 1, a motor 1 (201) is fixedly provided at the upper end of the bottom plate (2), the motor 1 (201) is connected to a pulley 1 on the right side, the pulley 1 on the right side is connected to a pulley 1 on the left side via a conveyor belt 3 (202), the upper side of the conveyor belt 3 (202) is connected to a positioning base shell (203), the positioning base shell (203) is slidably arranged at the upper end of the bottom plate (2) in the left-right direction, the upper end of the positioning base shell (203) is used to place a sample tube rack (9), and the sample tube rack (9) is correspondingly arranged at the lower side of the sampling needle (106).

4. A flow cytometer according to claim 2, characterized in that: The liquid path assembly comprises a liquid path chamber (3) disposed inside the lower side of a main machine housing (7), wherein the liquid path chamber (3) is provided with a peristaltic pump 1 (301), a peristaltic pump 2 (312), a diaphragm pump 1 (302), a diaphragm pump 2 (313), a filter 1 (304), a filter 2 (310), a buffer tank (305), a plunger pump (306), a solenoid valve group (307) and a three-way solenoid valve (311), and a liquid inlet pipe (303) and a liquid outlet pipe (311) are disposed inside the liquid path chamber (3), respectively. The inlet of the liquid inlet pipe (303) is connected to the sheath liquid container through the liquid delivery pipe 1, the outlet of the liquid inlet pipe (303) is connected to the inlet of the diaphragm pump 1 (302) through the liquid delivery pipe 2, the outlet of the diaphragm pump 1 (302) is connected to the inlet of the electromagnetic valve group (307) through the liquid delivery pipe 3, the outlet 1 of the electromagnetic valve group (307) is connected to the inlet of the filter 1 (304) through the connecting pipe 1, and the outlet 2 of the electromagnetic valve group (307) is connected to the inlet of the filter 2 (310) through the connecting pipe 2. The outlet of filter 1 (304) and the outlet of filter 2 (310) are respectively connected to the inlet of buffer tank (305) through a liquid delivery pipe 11, the outlet 1 of buffer tank (305) is connected to the inlet of peristaltic pump 1 (301) through a liquid delivery pipe 4, the outlet of peristaltic pump 1 (301) is connected to a liquid delivery pipe 5, the liquid delivery pipe 5 is connected to the inlet of plunger pump (306), the outlet of plunger pump (306) is connected to port 1 of three-way solenoid valve (311) through a liquid delivery pipe 6, the outlet of three-way solenoid valve (311) is connected to the inlet of plunger pump (306), and the outlet of plunger pump (306) is connected to port 1 of three-way solenoid valve (311) through a liquid delivery pipe 6. Port 2 is connected to the sampling needle (106) through a liquid delivery tube 7, port 3 of the three-way solenoid valve (311) is connected to inlet 1 of the flow chamber (308) through a liquid delivery tube 8, outlet 2 of the buffer tank (305) is connected to the inlet of peristaltic pump 2 (312) through a liquid delivery tube 9, the outlet of peristaltic pump 2 (312) is connected to inlet 2 of the flow chamber (308) through a liquid delivery tube 10 (315), and the outlet of the flow chamber (308) is connected to inlet 1 of the waste liquid container through a liquid delivery tube 12 and a liquid outlet pipe (309).

5. A flow cytometer according to claim 4, characterized in that: A collecting swab (314) is provided in the sampling cavity (1). The collecting swab (314) is correspondingly arranged on the lower side of the sampling needle (106). The collecting swab (314) is connected to the inlet of the diaphragm pump (313) through the liquid delivery tube thirteen. The outlet of the diaphragm pump (313) is connected to the inlet of the waste liquid container through the liquid delivery tube fourteen and the liquid outlet pipe (309).

6. A flow cytometer according to claim 5, characterized in that: The stable optical platform component comprises a cavity 2 arranged inside the upper side of a main machine housing (7); a partition is fixedly arranged between the cavity 2 and the sampling chamber (1); a mounting hole is penetrated through the partition and a liquid supply pipe 10 (315) passes through the partition; a stable shell (4) is arranged on the right side of the upper end of the partition; three lasers (401) are arranged inside the stable shell (4) along the front-to-back direction; the three lasers (401) and the three telephoto lenses (404) are arranged in a one-to-one correspondence; the three telephoto lenses (404) and the three dichroic mirrors (402) are arranged in a one-to-one correspondence; the lasers (401), the telephoto lenses (404) and the dichroic mirrors (402) are arranged in sequence from left to right; the dichroic mirrors ( A flow chamber (308) is provided on the front side of the stabilizing shell (402), a rectifying block (403) is provided between the flow chamber (308) and the frontmost dichroic mirror (402), the left side of the flow chamber (308) is provided with a side scattering lens (405), the front side of the flow chamber (308) is provided with a front scattering lens (407), the front scattering lens (407) is connected with a front scattering detector (408), the side scattering lenses (405) are respectively electrically connected with the detection module components, a vibration damping plate (10) is provided at the lower end of the stabilizing shell (4), a buffer column (406) is provided at the top corner of the lower end of the vibration damping plate (10), and the buffer column (406) is fixedly connected with the partition.

7. A flow cytometer according to claim 6, characterized in that: The detection module assembly comprises three spectrum detectors (5), the three spectrum detectors (5) are electrically connected to the side diffusion lens (405) respectively, and the three spectrum detectors (5) are installed on the left side of the upper end of the partition.

8. A flow cytometer according to claim 6, characterized in that: The heat dissipation assembly comprises a main machine housing (7) having an air inlet at the front end, the air inlet being connected to the heat dissipation housing (6), an air outlet at the upper side of the rear end of the main machine housing (7), the air outlet being arranged corresponding to the heat dissipation housing (6), an exhaust fan (13) being installed at the rear end of the heat dissipation housing (6), the heat dissipation housing (6) being installed on the left side of the lower end of the partition, heat dissipation holes being penetrated at the front and rear ends of the heat dissipation housing, and a plurality of heat dissipation plates being evenly arranged in the heat dissipation holes along the left and right directions.

9. A flow cytometer according to claim 6, characterized in that: The power circuit assembly comprises a board box (11), a second exhaust fan (14) is installed at the rear end of the board box (11), a second air outlet is provided at the lower side of the rear end of the main machine housing (7), the second air outlet is correspondingly connected to the second exhaust fan (14), a heat dissipation base plate (12) is provided at the lower end of the board box (11), and a second air inlet (701) arranged at the left end of the main machine housing (7) is connected to the lower side of the main machine housing (7).

10. A flow cytometer according to claim 9, characterized in that: An exhaust fan three (15) is installed at the rear end of the main machine housing (7), the box (11) and the exhaust fan three (15) are distributed on the left and right inside the main machine housing (7), and an air outlet three is provided at the rear end of the main machine housing (7).

Images