Detection device for preparing recombinant adenovirus for expressing African swine fever protein

Abstract

The invention discloses a detection device for preparing recombinant adenovirus for expressing African swine fever protein, and relates to the technical field of biological detection equipment. Comprising an electrophoresis box, a gel plate is installed on the electrophoresis box, the gel plate is used for placing gel, two sliding grooves are formed in the gel plate, and two inserting plates are installed on the gel plate; the trepanning frame is installed on the gel plate, the two ends of the trepanning frame slide in the adjacent sliding grooves respectively, piston rows are connected into the trepanning frame in a limited and sliding mode, an installation frame is connected to the trepanning frame in a sliding mode, and the two ends of the installation frame slide in the adjacent sliding grooves respectively. And a plurality of inserting blocks are fixedly connected to the side, away from the trepanning frame, of the mounting frame. On the premise of not changing the volume of the gel sample adding hole, the communication area of the sample adding hole is reduced, and the sample is inhibited from diffusing out of the sample adding hole, so that the reliability of an electrophoresis result is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological detection equipment, and in particular to a detection device for preparing a recombinant adenovirus expressing African swine fever protein. Background Art

[0002] African swine fever (ASF) is an acute, hemorrhagic, highly contagious disease caused by the African swine fever virus (ASFV) infecting domestic pigs and various wild boars. In order to develop a vaccine against African swine fever, it is necessary to prepare a recombinant adenovirus that can express African swine fever protein. The preparation of recombinant adenovirus mainly includes the key steps of vector construction, recombinant plasmid construction, transfection, virus amplification, purification and titer determination. At the end of the above key steps, sampling detection is required to ensure the safety and effectiveness of the experiment. After the recombinant plasmid is constructed, it is necessary to verify whether the plasmid structure is correct. At this time, it is necessary to use restriction endonucleases to digest the recombinant plasmid, and then separate the enzyme digestion products by agarose gel electrophoresis and compare them with the expected fragment size to verify whether the plasmid structure is correct.

[0003] Agarose gel electrophoresis is an electrophoresis technique that uses agarose gel as a supporting medium. This technique is based on the principle of molecular sieves and can achieve separation based on the different movement speeds caused by differences in the molecular weight or shape of nucleic acid fragments. In this way, the movement distance of the separated nucleic acid fragments is compared with the movement distance of the expected fragments to determine whether the two are the same. After the gel is prepared, in order to maintain the wetness of the gel, the gel needs to be soaked in electrophoresis buffer, and then the sample (i.e., the separated enzyme cleavage product) and the reference (i.e., the expected nucleic acid fragment) are injected into the sample wells of the gel. In order to save the amount of agarose used, the sample wells are usually designed as low rectangular grooves, which makes the area of ​​the sample well connected to the outside world large. At the same time, since the density of the sample and the electrophoresis buffer is similar, the sample is easy to diffuse in the electrophoresis buffer, resulting in cross-contamination between the sample and the samples in other sample wells, affecting the final electrophoresis result. Summary of the invention

[0004] The present invention provides a detection device for preparing a recombinant adenovirus expressing African swine fever protein, so as to overcome the disadvantage that during gel electrophoresis, samples are easily diffused in the electrophoresis buffer, resulting in cross contamination between adjacent samples and affecting the electrophoresis results.

[0005] The technical implementation scheme of the present invention is: a detection device for preparing a recombinant adenovirus expressing African swine fever protein, comprising an electrophoresis box, a gel plate installed on the electrophoresis box, the gel plate used to place gel, two sliding grooves are provided on the gel plate, and two plug plates are installed on the gel plate; a hole frame installed on the gel plate, the two ends of the hole frame slide in adjacent sliding grooves respectively, a piston row is slidingly connected in the hole frame, a mounting frame is slidingly connected on the hole frame, the two ends of the mounting frame slide in adjacent sliding grooves respectively, a plurality of plug blocks are fixedly connected to the side of the mounting frame away from the hole frame, the plug blocks are used to form sample loading holes on the gel surface, the plug blocks are sealingly and slidingly connected to the piston row, the plug blocks cooperate with the piston row to form a power chamber, an elastic body is fixedly connected to the side of the plug block away from the hole frame, the elastic body is used to expand the volume inside the sample loading hole on the gel, the plug blocks cooperate with the adjacent elastic bodies to form an expansion chamber, and the power chamber is connected to the adjacent expansion chamber.

[0006] Furthermore, a rubber block is fixedly connected in the sliding groove, the rubber block is limitedly matched with the hole opening frame, and the elastic coefficient of the rubber block is greater than the elastic coefficient of the elastic body.

[0007] Furthermore, a knob is threadedly connected to the perforated frame, and the knob is extrusion-fitted with the piston row.

[0008] Furthermore, the width of the sliding slot is equal to the width of the end of the opening frame and the mounting frame.

[0009] Furthermore, a perforated arc block is fixed in the sliding groove, the perforated arc block is squeezed and matched with the mounting frame, and the distance between the perforated arc block and the adjacent rubber block in the vertical direction is less than the sum of the thicknesses of the end of the perforated frame and the end of the mounting frame.

[0010] Furthermore, two U-shaped grooves are arranged on the gel plate, the plug plate slides in adjacent U-shaped grooves, a sealing strip is fixed in the U-shaped groove, the sealing strip is squeezed and matched with the adjacent plug plate, a sealing arc block is fixed in the U-shaped groove, and a limit groove is arranged on the plug plate to limit the adjacent sealing arc block.

[0011] Furthermore, the peripheral side of the sealing strip does not contact the adjacent U-shaped groove.

[0012] Furthermore, the plug plate is slidably connected with symmetrically distributed extrusion pieces, an extrusion block is fixedly connected to the plug plate near the adjacent extrusion piece, the extrusion block is extruded and matched with the adjacent extrusion piece, and the plug plate and the adjacent extrusion piece are jointly fixed with an elastic strip on one side away from the knob.

[0013] Furthermore, the extrusion piece is provided with a pushing portion and a supporting portion, and the supporting portion is used to keep the adjacent extrusion piece stable.

[0014] Furthermore, it also includes: a limit bar, which is fixed to a position on the gel plate away from the sliding groove, and the limit bar is coplanar with a side of the adjacent U-shaped groove close to the sliding groove, and the limit bar is used to provide support for the gel.

[0015] In summary, the present application includes at least one of the following beneficial technical effects: the present invention reduces the connected area of ​​the sample loading hole without changing the volume of the gel sample loading hole, thereby inhibiting the diffusion of the sample out of the sample loading hole, thereby improving the reliability of the electrophoresis result; the movement of the hole opening frame and the mounting frame is guided by the sliding groove to avoid disturbing the solution to form bubbles, while avoiding damage to the sample loading hole, thereby ensuring the accuracy of the final electrophoresis result; the gap between the plug plate and the U-shaped groove is filled with a sealing strip, thereby improving the sealing performance between the plug plate and the gel plate and preventing leakage of the solution; by making the width of the sealing strip smaller than the width of the U-shaped groove, water droplets are prevented from accumulating between the sealing strip and the gel plate, thereby facilitating the cleaning of the gel plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention; Figure 2 It is a schematic diagram of the three-dimensional structure of the gel plate, the plug plate and the perforated frame of the present invention; Figure 3 The present invention is attached Figure 2 The enlarged view of point A in the middle; Figure 4 It is a sectional view of the three-dimensional structure of the elastic body of the present invention when it expands; Figure 5 This is a schematic diagram of the three-dimensional structure of the elastomer of the present invention when it is not expanded; Figure 6 It is a schematic diagram of a three-dimensional structure in which the extrusion block and the plug plate are coplanar with each other; Figure 7 It is a schematic diagram of the three-dimensional structure of the gel plate and the sealing strip of the present invention; Figure 8 This is a schematic diagram of the three-dimensional structure of the extrusion block of the present invention being located inside the plug board; Fig. 9 It is a schematic diagram of the three-dimensional structure of the extrusion part of the present invention; Fig.10 It is a schematic diagram of the three-dimensional structure of the gel plate and the limiting strip of the present invention.

[0017] The numbers in the figure are: 1-electrophoresis box, 101-terminal, 102-electrode plate, 2-gel plate, 201-sliding groove, 202-U-shaped groove, 3-insertion plate, 4-opening frame, 5-piston row, 6-mounting frame, 7-insertion block, 701-power chamber, 8-elastic body, 801-expansion chamber, 9-rubber block, 10-knob, 11-opening arc block, 12-sealing strip, 13-sealing arc block, 131-limiting groove, 14-extrusion piece, 141-pushing part, 142-supporting part, 15-extrusion block, 16-elastic strip, 17-limiting strip. DETAILED DESCRIPTION

[0018] The following will be combined with the attached Figure 1 To Attachment Fig.10 The present invention is described in detail, and the technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] A detection device for preparing a recombinant adenovirus expressing African swine fever protein, see Figure 1-Figure 5 , including an electrophoresis box 1, on which a gel plate 2 is installed, the gel plate 2 is used to place gel, the gel plate 2 is provided with two sliding grooves 201, and the gel plate 2 is provided with two plug-in plates 3; a perforated frame 4, installed on the gel plate 2, the two ends of the perforated frame 4 slide in the adjacent sliding grooves 201 respectively, the perforated frame 4 is limitedly slidably connected with a piston row 5, the perforated frame 4 is slidably connected with a mounting frame 6, the two ends of the mounting frame 6 slide in the adjacent sliding grooves 201 respectively, and a plurality of plug-in blocks 7 are fixedly connected to the side of the mounting frame 6 away from the perforated frame 4, and the plug-in blocks 7 It is used to form sample loading holes on the surface of the gel. The plug block 7 is sealingly and slidably connected with the piston row 5. The plug block 7 and the piston row 5 cooperate to form a power chamber 701. An elastic body 8 is fixedly connected to the side of the plug block 7 away from the hole frame 4. The elastic body 8 is used to expand the volume inside the sample loading hole on the gel. The plug block 7 cooperates with the adjacent elastomer 8 to form an expansion chamber 801. The power chamber 701 is connected to the adjacent expansion chamber 801. A rubber block 9 is fixedly connected to the sliding groove 201. The rubber block 9 is limitedly matched with the hole frame 4. The elastic coefficient of the rubber block 9 is greater than the elastic coefficient of the elastomer 8.

[0020] In the above scheme, the purpose is to suppress the diffusion of samples in the sample loading holes by reducing the opening area of ​​the sample loading holes; the left and right sides of the electrophoresis box 1 are both equipped with terminal posts 101 and electrode plates 102, and the terminal posts 101 are electrically connected to the adjacent electrode plates 102, and the terminal posts 101 are used to communicate with the external instrument electrophoresis instrument; the gel plate 2 cooperates with the two plug plates 3 to form a space for the agarose solution (hereinafter referred to as the solution) to solidify; the lower part of the plug block 7 is immersed in the solution to form the sample loading hole when the solution solidifies into a gel; the lower side of the piston row 5 is composed of a plurality of elliptical pistons, and the elliptical pistons on the piston row 5 can form a sealing fit with the adjacent plug blocks 7 through rubber rings, etc.; the elastic body 8 The material can be natural latex, etc., which has excellent elasticity and is easy to separate from the gel; a medium is stored in the power chamber 701 and the expansion chamber 801, and the medium can be liquid to ensure the reliability of transmission; the difference in elastic coefficient between the rubber block 9 and the elastomer 8 can be adjusted by material, density, etc., and the rubber block 9 can be two groups symmetrically distributed front and back, with two rubber blocks 9 in each group, and the two rubber blocks 9 are symmetrically distributed, which improves the uniformity of force when the opening frame 4 is limited by the rubber block 9; by reducing the connecting area of ​​the sample loading hole without changing the volume of the gel sample loading hole, the diffusion of the sample outside the sample loading hole is suppressed, thereby improving the reliability of the electrophoresis result.

[0021] See also Figure 4 and Figure 5 A knob 10 is threadedly connected to the hole frame 4 , and the knob 10 is extruded and matched with the piston row 5 .

[0022] In the above scheme, the purpose is to change the expansion volume of the elastomer 8 by adjusting the position of the knob 10; the knob 10 is composed of a threaded rod and a disc, and the side of the disc can be provided with annularly distributed grooves to increase the surface roughness of the disc on the knob 10; when the hole-opening frame 4 moves downward, the hole-opening frame 4 drives the knob 10 to move downward, and the knob 10 pushes the piston row 5 to move downward, and the piston row 5 squeezes the medium in the power chamber 701 into the adjacent expansion chamber 801, so that the elastomer 8 expands; after the piston row 5 stops moving, the volume that the elastomer 8 can reach is referred to as the set volume.

[0023] The steps of adjusting the set volume of the elastomer 8 by changing the position of the knob 10 are as follows: initially, the upper side of the piston row 5 fits with the threaded rod of the knob 10. At this time, the hole-opening frame 4 directly drives the piston row 5 to move when moving. The moving distance of the piston row 5 is equal to the moving distance of the hole-opening frame 4. The volume of the elastomer 8 after expansion is the largest. When it is necessary to reduce the volume of the sample addition hole, it is only necessary to turn the knob 10 so that there is a gap between the threaded rod of the knob 10 and the piston row 5. In the process of the hole-opening frame 4 moving downward, the knob 10 is first driven to contact the piston row 5, and then the piston row 5 can be pushed downward, so that the moving distance of the piston row 5 is reduced, that is, the volume of the medium entering the expansion chamber 801 is reduced, and the volume of the elastomer 8 after expansion is reduced; in this way, the volume of the sample addition hole can be changed by turning the knob 10.

[0024] See also Figure 3 The width of the sliding groove 201 is equal to the width of the end of the opening frame 4 and the mounting frame 6.

[0025] In the above scheme, the purpose is to limit the hole frame 4 and the mounting frame 6 by the sliding groove 201, so as to keep the moving direction of the hole frame 4 and the mounting frame 6 unchanged, thereby avoiding damage to the gel; the ends of the hole frame 4 and the mounting frame 6 refer to the front and rear ends of the hole frame 4 and the mounting frame 6; the hole frame 4 and the mounting frame 6 are guided by the sliding groove 201, so that the hole frame 4 and the mounting frame 6 are moved along the sliding groove 201, so as to facilitate the smooth insertion of the plug 7 into the solution, reduce the probability of generating bubbles in the solution, and facilitate the smooth pulling out of the gel after the gel is formed, so as to avoid the damage of the sample wells due to swinging in the process of pulling the plug 7 out of the gel, thereby accelerating the cross infection of the samples in the sample wells.

[0026] See also Figure 3 A perforated arc block 11 is fixedly connected in the sliding groove 201, and the perforated arc block 11 is squeezed and matched with the mounting frame 6. The distance between the perforated arc block 11 and the adjacent rubber block 9 in the vertical direction is less than the sum of the thicknesses of the end of the perforated frame 4 and the end of the mounting frame 6.

[0027] In the above scheme, the problem that the liquid level at the position where the solution contacts the plug 7 is lower than the liquid level at other positions due to the surface tension and viscosity of the solution is solved; in the process of inserting the plug 7 into the solution, the position where the solution contacts the plug 7 will be lower than the liquid level at other positions of the solution due to the viscosity of the solution. As time goes by, the solution will gradually infiltrate other positions of the plug 7 under the action of its own gravity until the position where the solution contacts the plug 7 is flush with the liquid surface (for example, a wooden stick is inserted into cement). However, as time goes by, the temperature of the solution gradually decreases, and the solution will gradually turn into a gel state. The change causes the speed of the solution infiltrating the plug 7 to gradually decrease, which may easily cause the height of the edge of the sample loading hole to be lower than the height of the gel after the solution forms a gel; the opening arc block 11 is made of elastic metal material, and can be slightly deformed when the opening arc block 11 is squeezed, so that the plug 7 is inserted into the solution to a certain depth, so that the height of the solution infiltrating the surface of the plug 7 is increased, and then the plug 7 is pulled upward to the above-mentioned depth, eliminating the step of the solution gradually infiltrating the plug 7 after the plug 7 is stationary, thereby removing the concave liquid surface formed at the position where the solution contacts the plug 7 due to the surface tension, and improving the uniformity of the gel thickness.

[0028] The operation steps of the above scheme are as follows: when performing gel electrophoresis, the two plug plates 3 are installed at the corresponding positions on the gel plate 2, and then the prepared solution is poured into the space formed by the gel plate 2 and the two plug plates 3 to wait for solidification. During the waiting period for solidification, the hole-opening frame 4, the piston row 5, the mounting frame 6, the plug block 7 and the elastic body 8 are moved in the direction close to the sliding groove 201, and the mounting frame 6 and the hole-opening frame 4 enter the sliding groove 201 in turn and squeeze the rubber block 9 in turn, and then the mounting frame 6 contacts the hole-opening arc block 11 and stops moving (this When both the plug block 7 and the elastic body 8 stop moving and the solution completely covers the elastic body 8), the hole frame 4 continues to move downward, and the hole frame 4 pushes the piston row 5 downward through the knob 10. The piston row 5 moves downward relative to the multiple plug blocks 7, and squeezes the medium in the power chamber 701 into the adjacent expansion chamber 801, so that all the elastic bodies 8 expand, until the end of the hole frame 4 contacts the end of the mounting frame 6, and the hole frame 4 is stationary relative to the mounting frame 6 (at this time, the elastic body 8 expands to the set volume, and the hole frame 4 passes over the rubber block 9).

[0029] Continue to press the hole frame 4 downward, so that the hole frame 4 squeezes the hole arc block 11 through the mounting frame 6, and the hole arc block 11 is deformed a short distance. At the same time, the mounting frame 6 drives the plug block 7 to move downward, so that the height of the solution infiltrating the surface of the plug block 7 increases. At this time, the hole frame 4 is released, and the hole frame 4 and the mounting frame 6 are pushed upward under the resetting action of the hole arc block 11, until the hole arc block 11 is reset and the hole frame 4 is stopped by the rubber block 9. At the same time, the plug block 7 moves upward. At this time, the height of the position where the solution adheres to the plug block 7 is higher than the liquid surface, eliminating the step of gradually infiltrating the plug block 7 with the solution, and accelerating the speed at which the liquid surface of the solution returns to a flat state. After the agarose solution forms a gel, the two plug plates 3 are removed, and the hole frame 4 is lifted up, and the hole frame 4 First, the medium in the expansion chamber 801 is driven by the piston row 5 to enter the adjacent power chamber 701 to reset the elastic body 8. Then, the hole-opening frame 4 drives the mounting frame 6 to move upward, and the driving plug 7 loses contact with the gel. At this time, a sample loading hole with a connection area equal to the lower side area of ​​the plug 7 is formed on the surface of the gel. The electrophoresis buffer is injected into the electrophoresis box 1, and the electrophoresis buffer is made to cover the gel. Then, the enzyme cleavage product and the comparison sample (i.e., the expected fragment) are respectively injected into different sample loading holes using a pipette, and the power is turned on to perform electrophoresis on them. The enzyme cleavage products of different lengths (i.e., nucleic acid fragments) are separated by using the different movement speeds of the enzyme cleavage products in the gel under the influence of the electric current. Finally, the gel is removed and the electrophoresis results are observed by a gel imager.

[0030] See also Figure 2 , Figure 6 and Figure 7 Two U-shaped grooves 202 are provided on the gel plate 2, and the plug plate 3 slides in the adjacent U-shaped grooves 202. A sealing strip 12 is fixedly connected in the U-shaped groove 202, and the sealing strip 12 is squeezed and matched with the adjacent plug plate 3. A sealing arc block 13 is fixedly connected in the U-shaped groove 202, and a limiting groove 131 is provided on the plug plate 3 to limit the adjacent sealing arc block 13.

[0031] In the above scheme, the sealing performance between the plug board 3 and the gel plate 2 is improved; the sealing strip 12 is made of flexible rubber material, and the sealing arc block 13 is made of elastic metal material; when the plug board 3 is inserted into the corresponding U-shaped groove 202, the plug board 3 squeezes the adjacent sealing strip 12 to deform, and the sealing strip 12 is used to fill the gap between the plug board 3 and the U-shaped groove 202, thereby improving the sealing performance between the plug board 3 and the gel plate 2. As the plug board 3 moves downward, the plug board 3 squeezes the adjacent sealing arc block 13 to deform, until the upper side of the plug board 3 is coplanar with the upper side of the gel plate 2, the limiting groove 131 on the plug board 3 corresponds to the adjacent sealing arc block 13, and at this time, the sealing arc block 13 is reset under its own elastic action and limits the adjacent plug board 3.

[0032] See also Figure 7 , the peripheral side of the sealing strip 12 does not contact the adjacent U-shaped groove 202 .

[0033] In the above scheme, it is intended to facilitate the cleaning of the sealing strip 12 and the gel plate 2 after the electrophoresis is completed; a plurality of through holes communicating with the outside can be provided on the lower side of the U-shaped groove 202, so as to facilitate the discharge of the water droplets remaining in the U-shaped groove 202 after cleaning; the peripheral side of the sealing strip 12 is not in contact with the gel plate 2, that is, there is a gap between the sealing strip 12 and the gel plate 2 except for the two ends (see the attached Figure 7 ), to prevent water droplets from accumulating between the sealing strip 12 and the gel plate 2, so as to facilitate cleaning of the gel plate 2.

[0034] See also Figure 6 and Figure 8 The plug plate 3 is slidably connected with symmetrically distributed extrusion pieces 14, and an extrusion block 15 is fixedly connected to the plug plate 3 near the adjacent extrusion piece 14. The extrusion block 15 is extruded and matched with the adjacent extrusion piece 14, and an elastic strip 16 is fixedly connected to the plug plate 3 and the adjacent extrusion piece 14 on the side away from the knob 10.

[0035] In the above scheme, the friction between the plug board 3 and the sealing strip 12 is reduced, and the service life of the sealing strip 12 is extended; there can be multiple extrusion blocks 15, which are used to improve the uniformity of the force applied to each part of the extrusion piece 14; the sealing strip 12 forms two vertical parts and one horizontal part in the adjacent U-shaped groove 202; when the plug board 3 moves down along the adjacent U-shaped groove 202, the elastic strip 16 and the extrusion piece 14 first contact the horizontal part of the sealing strip 12, and as the plug board 3 continues to move down, the extrusion piece 14 moves up relative to the adjacent plug board 3, and the extrusion piece 14 is squeezed by the adjacent extrusion block 15. It moves downward toward the direction of the adjacent vertical portion of the sealing strip 12 and finally contacts the sealing strip 12, extruding the vertical portion of the sealing strip 12 to deform, extruding the sealing strip 12 to deform and fill the gap between the front and rear sides of the plug board 3 and the adjacent U-shaped groove 202 (finally the side of the extrusion piece 14 away from the adjacent extrusion block 15 is coplanar with the side of the adjacent plug board 3); when the plug board 3 moves downward, the contact area between the plug board 3 and the sealing strip 12 is reduced, the extrusion force on the vertical portion of the sealing strip 12 is reduced, and the wear on the sealing strip 12 is reduced, thereby extending the service life of the sealing strip 12.

[0036] See also Fig. 9 The extrusion piece 14 is provided with a pushing portion 141 and a supporting portion 142 , and the supporting portion 142 is used to keep the adjacent extrusion piece 14 stable.

[0037] In the above scheme, the purpose is to improve the stability of the plug board 3; when the extrusion piece 14 moves upward relative to the extrusion block 15, the extrusion block 15 first contacts the pushing portion 141 (at this time, due to the effect of the inclined surface, the extrusion block 15 is subjected to the upward force given by the extrusion piece 14, which increases the force of the plug board 3 on the adjacent sealing arc block 13). When the extrusion piece 14 moves away from the side of the adjacent extrusion block 15 and is coplanar with the side of the adjacent plug board 3, the extrusion block 15 contacts the supporting portion 142. At this time, the extrusion block 15 is no longer subjected to the upward force, thereby reducing the force of the plug board 3 on the adjacent sealing arc block 13 and improving the stability of the plug board 3.

[0038] See also Fig.10 , and also includes: a limiting strip 17, which is fixed to a position on the gel plate 2 away from the sliding groove 201, and the limiting strip 17 is coplanar with a side of the adjacent U-shaped groove 202 close to the sliding groove 201, and the limiting strip 17 is used to provide support for the gel.

[0039] In the above scheme, the stability of the gel when the electrophoresis buffer is injected is improved; when the electrophoresis buffer is injected into the electrophoresis box 1, it is necessary to repeatedly pour the electrophoresis buffer to the left and right sides of the electrophoresis box 1 so that the electrophoresis buffer on the left and right sides of the electrophoresis box 1 will not flow to each other, thereby reducing the influence of the pouring of the electrophoresis buffer on the position of the gel. This method is complicated to operate and is not conducive to improving the electrophoresis rate; the present scheme fixes the gel by the limiting strip 17 and the insert 7 together to prevent the flow of the electrophoresis buffer from affecting the position of the gel, so that it is only necessary to pour the electrophoresis buffer to one side of the electrophoresis box 1, saving the operation steps. step, which is beneficial to improving the electrophoresis rate; the sum of the height of the limiting strip 17 and the height of the sample loading hole should be less than the thickness of the gel to avoid affecting the movement of the sample in the gel; after the solution forms a gel, the two plug plates 3 are removed (at this time, the plug block 7 does not need to be removed), and then the electrophoresis buffer is poured to the left part of the electrophoresis box 1. The electrophoresis buffer submerges the gel and flows to the right part of the electrophoresis box 1 through the gaps of multiple plug blocks 7. In this process, the multiple plug blocks 7 and the limiting strip 17 jointly provide support for the gel to prevent the gel from moving when the electrophoresis buffer flows. When the electrophoresis buffer completely submerges the gel, the plug block 7 is removed and electrophoresis is started.

[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A detection device for preparing a recombinant adenovirus expressing African swine fever protein, characterized in that: The electrophoresis box (1) comprises a gel plate (2) mounted on the electrophoresis box (1), the gel plate (2) being used to place gel, the gel plate (2) being provided with two sliding grooves (201), and the gel plate (2) being provided with two plug plates (3); A hole frame (4) is mounted on the gel plate (2), the two ends of the hole frame (4) slide in adjacent sliding grooves (201) respectively, a piston row (5) is slidably connected in the hole frame (4), a mounting frame (6) is slidably connected on the hole frame (4), the two ends of the mounting frame (6) slide in adjacent sliding grooves (201) respectively, a plurality of plug blocks (7) are fixedly connected to a side of the mounting frame (6) away from the hole frame (4), and the plug blocks (7) are used to shape the surface of the gel. The plug block (7) is sealingly and slidably connected to the piston row (5), and the plug block (7) and the piston row (5) cooperate to form a power chamber (701). An elastic body (8) is fixedly connected to a side of the plug block (7) away from the hole frame (4), and the elastic body (8) is used to expand the volume inside the sample loading hole on the gel. The plug block (7) cooperates with the adjacent elastic body (8) to form an expansion chamber (801), and the power chamber (701) is connected to the adjacent expansion chamber (801).

2. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 1, characterized in that: A rubber block (9) is fixedly connected in the sliding groove (201), the rubber block (9) is limitedly matched with the hole opening frame (4), and the elastic coefficient of the rubber block (9) is greater than the elastic coefficient of the elastic body (8).

3. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 1, characterized in that: A knob (10) is threadedly connected to the hole opening frame (4), and the knob (10) is extruded and matched with the piston row (5).

4. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 3, characterized in that: The width of the sliding groove (201) is equal to the width of the end portions of the hole opening frame (4) and the mounting frame (6).

5. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 3, characterized in that: A perforated arc block (11) is fixedly connected in the sliding groove (201), the perforated arc block (11) is extruded and matched with the mounting frame (6), and the distance between the perforated arc block (11) and the adjacent rubber block (9) in the vertical direction is less than the sum of the thicknesses of the end of the perforated frame (4) and the end of the mounting frame (6).

6. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 1, characterized in that: The gel plate (2) is provided with two U-shaped grooves (202), the plug plate (3) slides in adjacent U-shaped grooves (202), a sealing strip (12) is fixedly connected in the U-shaped groove (202), the sealing strip (12) is pressed and matched with the adjacent plug plate (3), a sealing arc block (13) is fixedly connected in the U-shaped groove (202), and a limiting groove (131) is provided on the plug plate (3) and is limitedly matched with the adjacent sealing arc block (13).

7. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 6, characterized in that: The peripheral side of the sealing strip (12) does not contact the adjacent U-shaped groove (202).

8. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 6, characterized in that: The plug plate (3) is slidably connected to symmetrically distributed extrusion pieces (14); an extrusion block (15) is fixedly connected to a position of the plug plate (3) close to an adjacent extrusion piece (14); the extrusion block (15) is extruded and matched with the adjacent extrusion piece (14); and an elastic strip (16) is fixedly connected to the plug plate (3) and the adjacent extrusion piece (14) on a side away from the knob (10).

9. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 8, characterized in that: The extrusion piece (14) is provided with a pushing portion (141) and a supporting portion (142), and the supporting portion (142) is used to keep the adjacent extrusion piece (14) stable.

10. A detection device for preparing a recombinant adenovirus expressing African swine fever protein according to claim 6, characterized in that: Also included are: A limit strip (17), the limit strip (17) being fixedly connected to a position on the gel plate (2) away from the sliding groove (201), the limit strip (17) being coplanar with a side of an adjacent U-shaped groove (202) close to the sliding groove (201), and the limit strip (17) being used to provide support for the gel.

Images