Rice pollen collection device and migration determination method

Abstract

The invention discloses a rice pollen collection device and a migration determination method, and relates to the technical field of agricultural planting, the rice pollen collection device comprises a fixing assembly, an adjusting assembly and a collection assembly; the fixing assembly is used for being fixed in a collection scene; at least three sampling points are randomly selected to install pollen collecting devices in the test area with different flight parameter combinations according to the flight parameter setting condition of the unmanned aerial vehicle; sampling the glass slides for multiple times in a full-bloom stage, uniformly selecting a plurality of observation points on each glass slide in a plum blossom shape, and dyeing the observation points by using a 2% potassium iodide solution; according to the rice pollen collecting device and the migration measuring method, the method for judging the optimal pollination parameters only through the final yield and the maturing rate is improved, the judgment time is shortened, and the optimal pollination parameters of different types of unmanned aerial vehicles can be judged in a short time.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural planting, and in particular to a rice pollen collection device and a migration determination method. Background Art

[0002] Hybrid rice has important practical significance for increasing grain yield and ensuring food security. Assisted pollination is a key link in the hybrid rice seed production process and directly affects the seed production yield. There are three main existing assisted hybrid rice pollination methods: artificial assisted pollination, mechanical assisted pollination, and drone assisted pollination.

[0003] Artificial pollination, as a traditional pollination method, has the problems of high labor intensity and low production efficiency, and it is difficult to meet the requirements of modern seed production technology. Existing mechanical pollination is prone to plant damage, and there are few pollination machines in actual application, and the pollination effect is not ideal. As a new agricultural technology, drones can generate effective wind fields during flight, and their rational use can greatly improve pollination efficiency.

[0004] At present, the influence of the selection of hybrid rice drone pollination operation parameters on pollination effect, cost and benefit is unclear. Directly using a certain operation parameter directly will result in unclear effect and yield. Therefore, there is an urgent need for a method to quickly obtain the optimal drone pollination parameters to achieve precise control and improve the pollination rate and yield of hybrid rice. Summary of the invention

[0005] The purpose of the present invention is to solve the defects in the prior art and to propose a rice pollen collection device and a migration determination method.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A pollen collecting device comprises a fixing component, an adjusting component and a collecting component;

[0008] The fixing component is used to be fixed in the acquisition scene;

[0009] The adjusting component is provided with a first positioning clip and a second positioning clip, and the first positioning clip is detachably provided on the fixing component;

[0010] The collecting assembly is connected to the adjusting assembly via a second positioning clamp.

[0011] Furthermore, the collection device is a glass slide.

[0012] A method for measuring rice pollen migration, the above-mentioned pollen collection device, and comprising the following steps:

[0013] S1: Select the test site, select adjacent plots with the same growth conditions, flowering period and soil conditions for the male and female plants, and divide them into test areas.

[0014] S2: Arrange sampling points. According to the flight parameter settings of the UAV, randomly select at least three sampling points in the test area with different flight parameter combinations to install the pollen collection device;

[0015] S3: a collection assembly for collecting sampling points, sampling the slide multiple times during the blooming period, and evenly selecting multiple observation points in a plum blossom shape on each slide, and staining the observation points with a 2% potassium iodide solution;

[0016] S4: Observation and counting: Place the stained slide under a microscope for observation, and observe the pollen distribution at different observation points by moving the slide. Observe randomly at each observation point, count the number of pollen grains in the field of view, and take the average number of pollen grains in the observed effective field of view as the pollen distribution amount on a single slide;

[0017] S5: Perform data analysis on the number of pollen distribution recorded in each group and sort them according to the number of pollen. The more pollen the better, the more suitable the flight parameters of the drone are for pollination.

[0018] Furthermore, in step S2, the interval between the sampling points is 1 m; the line segment connecting the sampling points is perpendicular to the UAV track and is evenly distributed on both sides of the UAV track.

[0019] Furthermore, in step S3, sampling is performed once a day during the flowering period, and the number of sampling times is greater than or equal to three times.

[0020] Furthermore, in step S3, 5 observation points are evenly selected on each glass slide in a plum blossom shape, and the 5 observation points are stained with a 2% potassium iodide solution.

[0021] Furthermore, in step S4, random observations are made at each observation point, and the number of pollen grains in three fields of view is counted, resulting in a total of 15 valid counting fields of view. The number of pollen grains in the observed 15 valid fields of view is averaged as the pollen distribution amount on a single slide.

[0022] Beneficial Effects

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention provides a pollen migration determination method, which improves the method of determining the optimal pollination parameters only through final yield and fruit setting rate, shortens the determination time, and can determine the optimal pollination parameters of different types of drones in a short time. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0026] Figure 1 is an overall schematic diagram of the pollen collection device;

[0027] Figure 2 This is a schematic diagram of the pollen collection device and the position of the rice plant;

[0028] Figure 3 Schematic diagram of sampling point layout for drone-assisted pollination;

[0029] In the figure: 1. Fixing component; 2. Adjusting component; 3. Collecting component. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0031] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0032] Reference Figure 1 - Figure 3 , a pollen collecting device, comprising a fixing component 1, an adjusting component 2 and a collecting component 3;

[0033] The fixing component 1 is used to be fixed in the acquisition scene;

[0034] The adjusting component is provided with a first positioning clip and a second positioning clip, and the first positioning clip is detachably provided on the fixing component;

[0035] The collecting component is connected to the adjusting component through a second positioning clamp.

[0036] Wherein, the collecting device is a glass slide.

[0037] The fixing component is composed of a 1.5m long round tube; the adjusting component is composed of a universal clamp; the fixing component stands vertically at the field sampling point, one end of the adjusting component is fixed on the fixing component, and the other end is used for clamping the component device.

[0038] A rice pollen migration determination method utilizes the above-mentioned pollen collection device and comprises the following steps:

[0039] S1: Select the test site, select adjacent plots with the same growth conditions, flowering period and soil conditions for the male and female plants, and divide them into test areas.

[0040] S2: Arrange sampling points. According to the flight parameter settings of the UAV, randomly select at least three sampling points in the test area with different flight parameter combinations to install the pollen collection device;

[0041] S3: a collection assembly for collecting sampling points, sampling the slide multiple times during the blooming period, and evenly selecting multiple observation points in a plum blossom shape on each slide, and staining the observation points with a 2% potassium iodide solution;

[0042] S4: Observation and counting: Place the stained slide under a microscope for observation, and observe the pollen distribution at different observation points by moving the slide. Observe randomly at each observation point, count the number of pollen grains in the field of view, and take the average number of pollen grains in the observed effective field of view as the pollen distribution amount on a single slide;

[0043] S5: Perform data analysis on the number of pollen distribution recorded in each group and sort them according to the number of pollen. The more pollen the better, the more suitable the flight parameters of the drone are for pollination.

[0044] In other preferred embodiments, in step S2, the interval between the sampling points is 1 m; the line segment connecting the sampling points is perpendicular to the UAV track and is evenly distributed on both sides of the UAV track.

[0045] In other preferred embodiments, in step S3, sampling is performed once a day during the blooming period, and the number of sampling times is greater than or equal to three times.

[0046] In other preferred embodiments, in step S3, 5 observation points are evenly selected on each glass slide in a plum blossom shape, and the 5 observation points are stained with a 2% potassium iodide solution.

[0047] In other preferred embodiments, in step S4, random observations are performed at each observation point, and the number of pollen grains in three fields of view are counted, resulting in a total of 15 valid counting fields of view. The number of pollen grains in the observed 15 valid fields of view is averaged as the pollen distribution amount on a single slide.

[0048] Example 1: Operational performance of DJI T50 drone under different flight parameters

[0049] This experiment is designed with 9 test groups, including 2 factors and 3 levels, where the flight speed is 2m / s, 3m / s, 4m / s, and the flight altitude is 3.5m, 4.0m, 4.5m. The specific groups are as follows:

[0050] Table 1 Experimental treatment and design

[0051]

[0052]

[0053] Nine adjacent plots with the same growth conditions, flowering period and soil conditions for the male and female plants were selected for the experiment. Three more plots were selected as controls for artificial assisted pollination. Under the flight parameter combination selected in the early stage, the measured wind field width was 5-6m. In order to ensure that the downwash air flow field intersected when the drone was operating, thereby disturbing the rice canopy to the greatest extent to escape pollen, the track distance was set to 5m, and the sampling point arrangement was as follows: the drone operated autonomously according to the set flight parameters.

[0054] The test results are as follows:

[0055] As shown in Table 2, in the UAV-assisted pollination field production test, the average seed production yield under different UAV pollination parameters was 2.10 t / hm 2 The average fruit setting rate was 15.76%, the average 1000-grain weight was 21.94g, the average effective panicle was 21.62 holes / panicle, and the pollen distribution density was 3.67-5.44 / field of vision. The flight altitude and flight speed had a significant effect on the hybrid rice seed production yield, pollen distribution density and fruit setting rate. When the UAV flight speed was 3m / s and the flight altitude was 4m, it had the best pollination effect, and the final pollination yield was 2.64t / hm 2 .

[0056] Table 2 Hybrid rice yield and its components under different flight parameters

[0057]

[0058] Note: Different capital letters in the table indicate significant differences in flight speed at the p=0.05 level; Different lowercase letters in the table indicate significant differences in flight altitude at the p=0.05 level.

[0059] As shown in Table 3, the results of correlation analysis between the pollen electron microscope observation results in different experimental areas and the seed production yield in the experimental areas are shown in Table 3. The results show that there is a significant correlation between the field pollen distribution density and the seed production yield (p < 0.05).

[0060] Table 3 Correlation analysis between pollen distribution density and yield

[0061]

[0062] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A pollen collection device, characterized in that: It includes a fixing component, an adjusting component and a collecting component; The fixing component is used to be fixed in the acquisition scene; The adjusting component is provided with a first positioning clip and a second positioning clip, and the first positioning clip is detachably provided on the fixing component; The collecting assembly is connected to the adjusting assembly via a second positioning clamp.

2. A pollen collecting device according to claim 1, characterized in that: The collecting device is a glass slide.

3. A method for measuring rice pollen migration, characterized in that: The pollen collecting device according to claim 1 or 2 is used, and comprises the following steps: S1: Select the test site, select adjacent plots with the same growth conditions, flowering period and soil conditions for the male and female plants, and divide them into test areas. S2: Arrange sampling points. According to the flight parameter settings of the UAV, randomly select at least three sampling points in the test area with different flight parameter combinations to install the pollen collection device; S3: a collection assembly for collecting sampling points, sampling the slide multiple times during the blooming period, and evenly selecting multiple observation points in a plum blossom shape on each slide, and staining the observation points with a 2% potassium iodide solution; S4: Observation and counting: Place the stained slide under a microscope for observation, and observe the pollen distribution at different observation points by moving the slide. Observe randomly at each observation point, count the number of pollen grains in the field of view, and take the average number of pollen grains in the observed effective field of view as the pollen distribution amount on a single slide; S5: Perform data analysis on the number of pollen distribution recorded in each group and sort them according to the number of pollen. The more pollen the better, the more suitable the flight parameters of the drone are for pollination.

4. A rice pollen migration assay method according to claim 3, characterized in that: In step S2, the interval between the sampling points is 1 m; the line segment connecting the sampling points is perpendicular to the UAV track and is evenly distributed on both sides of the UAV track.

5. A rice pollen migration assay method according to claim 3, characterized in that: In the step S3, sampling is performed once a day during the blooming period, and the number of sampling times is greater than or equal to three times.

6. A rice pollen migration assay method according to claim 3, characterized in that: In step S3, five observation points are evenly selected on each glass slide in a plum blossom shape, and the five observation points are stained with a 2% potassium iodide solution.

7. A rice pollen migration assay method according to claim 3, characterized in that: In step S4, random observation is performed at each observation point, and the number of pollen grains in three fields of view is counted, resulting in 15 valid counting fields in total. The number of pollen grains in the observed 15 valid fields of view is averaged as the pollen distribution amount on a single slide.

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