Animal heart perfusion experiment device

Abstract

The invention relates to the technical field of animal experiments, in particular to an animal heart perfusion experiment device. The animal heart perfusion experiment device comprises a test bed, a shock pad and a support, the test bed is located at the upper end of the shock pad, and an operation table is formed at the upper end of the test bed; a liquid supply mechanism is mounted in the test bed; a test assembly is arranged outside the test bench. Meanwhile, a support is further arranged at the position, close to one side of the test bed, of the upper end of the shock pad, and a storage mechanism is installed on the support; according to the animal heart perfusion experiment device, a liquid supply mechanism is installed in a test bed, the arranged liquid supply mechanism comprises a replacement liquid tank, a flow pump, a humidifier, a flow guide pipe and an air catcher, replacement liquid can be humidified through the arranged humidifier, and the normal physiological function of the animal heart is maintained; the displacement liquid can be subjected to bubble removal treatment through the installed gas catcher; the purpose of humidifying the replacement liquid and removing bubbles is to improve the accuracy of the test result.

Description

Technical Field

[0001] The invention relates to the technical field of animal experiments, in particular to an animal heart perfusion experiment device. Background Art

[0002] There are two main types of cardiac perfusion experiments: isolated cardiac perfusion and in vivo cardiac perfusion; Isolated heart perfusion: The heart of an animal is removed from the body and placed in a specific physiological substitute fluid. Based on the purpose of the experiment and the characteristics of the animal, the heart is kept at a constant temperature, oxygenated or warmly perfused to establish an artificial environment that is basically similar to the internal environment of the animal body, so as to ensure that the heart maintains a normal state of activity. This experiment can eliminate the control of nerves and body fluids, making it easier to study changes in cardiac function and hemodynamics. In vivo cardiac perfusion: When the animal is anesthetized, the systemic circulation in the blood circulation is used to quickly flush the blood from the animal's body, and the tissue is pre-fixed before the animal dies, thereby keeping the specimen in a similar living state. This method is mainly used for tissue sectioning and morphological research; When conducting a cardiac perfusion experiment, a cardiac perfusion tool is generally used. Since the cardiac perfusion tools on the market are expensive, laboratories or independent research groups generally do not purchase them separately. Most researchers prefer to use foam boards, injection syringes, and scalp needles to make their own hand-push perfusion devices. However, the homemade perfusion devices have the disadvantages of being unable to recover liquid in real time and unstable perfusion rates. At the same time, two operators are required to collaborate in the experiment to obtain a better perfusion effect, which also causes a waste of manpower. The existing patent CN113973803A discloses an animal heart perfusion experimental device, which comprises a device body, an animal placement plate is arranged on the upper part of the device body, a perfusion liquid filter hole is also arranged on the upper part of the device body, the perfusion liquid filter hole is connected to the waste liquid collecting chamber, the waste liquid collecting chamber is connected to the waste liquid outlet pipe, the perfusion scalp needle is connected to the liquid outlet pipe, the liquid outlet pipe is connected to a micro pump, the micro pump is connected to the liquid inlet pipe port, and an animal fixing needle is arranged on the animal placement plate; the structure is simple and easy to use, which can effectively improve the rigor of the experiment, reduce the burden of the operator in the perfusion experiment, and improve the accuracy and efficiency of the heart perfusion.

[0003] However, the existing technology and patents also have some defects, for example: First, after the perfusion scalp needle is inserted into the heart of the experimental animal, the inclination angle position of the perfusion scalp needle and the gravity of the perfusion scalp needle automatically drop, and the perfusion scalp needle may change or fall off directly at the injection port of the animal tissue in the later stage, affecting the normal progress of the experiment and the test results; Second, at the same time, the replacement fluid used to inject into the animal heart is directly delivered by a micro pump, and the flow rate of the delivered replacement fluid is not easy to control, and it cannot be stopped immediately, and the flow rate of the replacement fluid is not easy to count, and there are a large number of bubbles in the supplied replacement fluid, which may enter the heart and interfere with the normal function of the heart, affecting the test results; In view of the problems in the above background technology, the present invention aims to provide an animal heart perfusion experimental device. Summary of the invention

[0004] The purpose of the present invention is to provide an animal heart perfusion experimental device to solve the problem of affecting the experimental results raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: An animal heart perfusion experimental device, the animal heart perfusion experimental device comprising: A test bench, a shock-absorbing pad and a bracket, wherein the test bench is located on the upper end of the shock-absorbing pad; Wherein, the upper end of the test bench forms an operating table, and a test animal is clamped and fixed on the operating table by a clamp; A guide plate is installed between the outer periphery of the operating table and the inner periphery of the test bench, and filter holes are provided on the surface of the guide plate; at the same time, a liquid collecting tank is formed inside the test bench, and the formed liquid collecting tank is funnel-shaped; the lower end of the filter hole provided on the surface of the guide plate faces the inside of the liquid collecting tank, and the bottom end of the liquid collecting tank is connected to the collecting pipe; a waste liquid collecting box is also movably installed inside the test bench, and a pull ring is installed on the outer side of the waste liquid collecting box; The test bench is also provided with a fluid supply mechanism for continuously, quantitatively and stably providing the humidified replacement fluid without bubbles required for the cardiac perfusion test of the clamped test animal; The outside of the test bench is provided with a test component for guiding the replacement fluid discharged by the fluid supply mechanism and injecting it into the test animal; At the same time, a bracket is provided on the upper end of the shock-absorbing pad near one side of the test bench, and a storage mechanism is installed on the bracket for placing the test component and adjusting the height position, eliminating the disadvantage that the operator needs to hold the test component for a long time to perform the cardiac perfusion test.

[0006] As a further solution of the present invention: the test assembly includes a guide end, a guide hose and an injection needle. The guide end is provided at one end of the guide hose, and the guide end is connected to a liquid supply mechanism installed inside the test bench; an injection needle is installed at the other end of the guide hose, and the guide hose is wrapped around the storage mechanism.

[0007] As a further solution of the present invention: the storage mechanism includes a mounting plate, a guide roller and an electric push rod; two guide rollers are movably installed on the outward side of the mounting plate in a linearly arranged manner, and a guide hose is wound around the outside of the guide roller; a slide plate is installed on the inner side of the mounting plate, a lifting groove is provided inside the bracket, a slide groove is provided on the inner wall of the lifting groove, and the slide plate installed on the inner side of the mounting plate is slidably installed inside the slide groove provided on the inner wall of the lifting groove; at the same time, the lower end of the slide plate is connected to the output end of the electric push rod, and the electric push rod is located inside the lifting groove.

[0008] As a further solution of the present invention: the liquid supply mechanism includes a displacement liquid tank, a flow pump, a humidifier, a flow guide pipe and an air trap, and the displacement liquid tank, the flow pump, the humidifier and the air trap are connected in sequence through the flow guide pipe.

[0009] As a further solution of the present invention: a control panel and a battery are also installed inside the test bench.

[0010] As a further solution of the present invention: a flow groove is formed inside the flow pump, a liquid inlet is opened on one side of the flow groove, and a liquid outlet is opened on the other side of the flow groove; at the same time, a first flow tooth and a second flow tooth are symmetrically installed inside the flow groove, the outer side of the second flow tooth is meshed with the outer side of the first flow tooth, and a driving shaft of the motor is installed in the middle of the first flow tooth, and the motor is located on the outside of the flow pump.

[0011] As a further solution of the present invention: a filter chamber is formed inside the air collector, and the filter chamber is in the shape of a cross. A liquid guide port is provided on one side of the filter chamber, and a liquid discharge port is provided on the other side of the filter chamber. A filter assembly is installed inside the filter chamber, and the filter assembly includes a filter basket, an inclined filter plate, a connecting rod and a sealing plug. The sealing plug is installed on the outside of the air collector, a connecting rod is provided at the middle lower end of the humidifier, an inclined filter plate is installed at the lower end of the connecting rod, and a filter basket is fitted on the lower end of the inclined filter plate. At the same time, the lower end of the filter chamber is connected to a sewage tank.

[0012] Compared with the prior art, the present invention has the following beneficial effects: Compared with the current animal heart perfusion experimental equipment, the animal heart perfusion experimental device has the following advantages: First, a bracket is provided at one side of the test bench, and a storage mechanism is installed on the bracket. The storage mechanism installed on the bracket is used to place and adjust the height of the test component, i.e., the perfusion scalp needle and the guide tube connected to the perfusion scalp needle, thereby eliminating the disadvantage that the operator needs to hold the test component for a long time to perform the cardiac perfusion test; and the correct injection port of the perfusion scalp needle for the animal tissue is not changed; Second, a flow pump is provided to replace the original micro pump, a flow slot is formed inside the flow pump, and a first flow tooth and a second flow tooth are symmetrically installed inside the flow slot, and the outer side of the second flow tooth is meshed with the outer side of the first flow tooth; the meshing rotation between the flow teeth promotes the flow of the replacement fluid, so that the replacement fluid inside the guide tube can be stopped immediately, and the flow rate can be easily controlled and counted; 3. A liquid supply mechanism is installed inside the test bench, which includes a replacement fluid tank, a flow pump, a humidifier, a guide tube and an air trap. The replacement fluid can be humidified by the humidifier to maintain the normal physiological function of the animal's heart; and the replacement fluid can be debubbled by the air trap. The purpose of humidifying and debubbling the replacement fluid is to improve the accuracy of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention.

[0014] Figure 1 The figure is a schematic structural diagram of an animal heart perfusion experiment device according to an embodiment of the present invention.

[0015] Figure 2 The present invention is a schematic diagram of the installation of a storage mechanism of an animal heart perfusion experiment device according to an embodiment of the present invention.

[0016] Figure 3 The figure is a schematic diagram of the internal structure of an animal heart perfusion experiment device according to an embodiment of the present invention.

[0017] Figure 4 The present invention is a schematic structural diagram of a flow pump of an animal heart perfusion experimental device according to an embodiment of the present invention.

[0018] Figure 5 The present invention is a schematic structural diagram of an air trap of a flow pump of an animal heart perfusion experimental device according to an embodiment of the present invention.

[0019] In the figure: 1- shock-absorbing pad, 2- test bench, 3- waste liquid collection box, 4- pull ring, 5- guide plate, 6- filter hole, 7- bracket, 8- lifting slot, 9- guide hose, 10- guide end, 11- injection needle, 12- test animal, 13- slide plate, 14- slide slot, 15- mounting plate, 16- guide roller, 17- electric push rod, 18- clamp, 19- collecting tank, 20- collecting pipe, 21- replacement liquid tank, 22- hydraulic meter, 23- flow guide tube, 24- flow pump, 25- humidifier, 26- control board, 27- battery, 28- air catcher, 29- liquid inlet, 30- flow slot, 31- first flow tooth, 32- driving shaft, 33- second flow tooth, 34- liquid outlet, 35- sealing plug, 36- connecting rod, 37- inclined filter plate, 38- filter basket, 39- sewage tank, 40- liquid guide port, 41- liquid outlet. DETAILED DESCRIPTION

[0020] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0021] Example See also Figure 1 and Figure 3 , an animal heart perfusion experiment device provided in an embodiment of the present invention, the animal heart perfusion experiment device comprising: A test bench 2, a shock-absorbing pad 1 and a bracket 7, wherein the test bench 2 is located at the upper end of the shock-absorbing pad 1, and the shock-absorbing pad 1 is used to buffer and reduce shock when the entire animal heart perfusion experimental device is placed, so as to ensure the stability of the test bench 2 during the test; The upper end of the test bench 2 forms an operating table, on which a test animal 12 is fixed by a clamp 18; the fixed test animal 12 is the subject of the cardiac perfusion test; a guide plate 5 is installed between the outer periphery of the operating table and the inner periphery of the test bench 2, and a filter hole 6 is provided on the surface of the guide plate 5; at the same time, a liquid collecting tank 19 is formed inside the test bench 2, and the formed liquid collecting tank 19 is funnel-shaped; the lower end of the filter hole 6 provided on the surface of the guide plate 5 faces the inside of the liquid collecting tank 19, and the bottom end of the liquid collecting tank 19 is connected to the collecting pipe 20; a waste liquid collecting tank 2 is also movably installed inside the test bench 2 The waste liquid collecting box 3 is provided with a pull ring 4 on the outside thereof; at the same time, the lower end of the collecting pipe 20 faces the inside of the waste liquid collecting box 3; when a cardiac perfusion test is performed on the operating table, the generated waste liquid falls on the surface of the guide plate 5, passes through the filter holes 6 opened on the surface of the guide plate 5 and enters the inside of the collecting tank 19; after the waste liquid enters the inside of the collecting tank 19, it is finally discharged through the collecting pipe 20 connected to the bottom end of the collecting tank 19 and is diverted and discharged into the inside of the waste liquid collecting box 3 for collection; at this time, the operator can exert force on the position of the pull ring 4, pull the inside of the waste liquid collecting box 3 out of the inside of the test bench 2, and pour out the collected waste liquid; The test bench 2 is also provided with a liquid supply mechanism, which is located outside the liquid collecting tank 19 inside the test bench 2; the liquid supply mechanism is used to continuously and quantitatively and stably provide the bubble-free humidified replacement fluid required for the cardiac perfusion test of the clamped test animal 12; A test assembly is provided outside the test bench 2, and the test assembly is connected to the outlet end of the liquid supply mechanism installed inside the test bench 2; the test assembly is used to guide the replacement fluid discharged by the liquid supply mechanism and inject it into the test animal 12; At the same time, a bracket 7 is provided on the upper end of the shock-absorbing pad 1 at a position close to one side of the test bench 2, and a storage mechanism is installed on the bracket 7; the storage mechanism installed on the bracket 7 is used to place the test component and adjust the height position, eliminating the disadvantage that the operator needs to hold the test component for a long time to perform the cardiac perfusion test; In an embodiment of the present invention, when the animal heart perfusion experiment device is used, the test animal 12 is placed on the operating table and fixed by the provided clamp 18; then the liquid supply mechanism is started, and the liquid supply mechanism stably outputs the replacement fluid required for the heart perfusion experiment, and the replacement fluid is continuously input into the test animal 12 through the connected test components; During the operation of the test assembly, the test assembly can be placed and the height position can be adjusted up and down through the storage mechanism installed on the bracket 7; See also Figure 1In one embodiment of the present invention, the test assembly includes a guide end 10, a guide hose 9 and an injection needle 11. The guide end 10 is provided at one end of the guide hose 9, and the guide end 10 is connected to a liquid supply mechanism installed inside the test bench 2; the injection needle 11 is installed at the other end of the guide hose 9, and the guide hose 9 is wound on the storage mechanism; In the embodiment of the present invention, when a cardiac perfusion test is performed on a test animal 12 fixed by a clamp 18 on an operating table through a set test assembly, a diversion end 10 installed at one end of a diversion hose 9 is connected to the outlet position of a liquid supply mechanism, and then the replacement fluid output by the liquid supply mechanism flows into the diversion hose 9 through the diversion end 10, and then the replacement fluid flows in the diversion hose 9 and finally is discharged from the injection needle 11 into the heart of the test animal 12, and a cardiac perfusion test is performed; during the test, the diversion hose 9 is placed and the upper and lower height positions are adjusted through the storage mechanism installed on the bracket 7; See also Figure 2 In one embodiment of the present invention, the storage mechanism includes a mounting plate 15, a guide roller 16 and an electric push rod 17; the mounting plate 15 is linearly arranged and movably mounted with two guide rollers 16 on one side facing outward, and the guide hose 9 is wound around the outside of the guide roller 16; a slide plate 13 is mounted on the inner side of the mounting plate 15, a lifting groove 8 is provided inside the bracket 7, a slide groove 14 is provided on the inner wall of the lifting groove 8, and the slide plate 13 mounted on the inner side of the mounting plate 15 is slidably mounted inside the slide groove 14 provided on the inner wall of the lifting groove 8; at the same time, the lower end of the slide plate 13 is connected to the output end of the electric push rod 17, and the electric push rod 17 is located inside the lifting groove 8; In the embodiment of the present invention, when the storage mechanism is used to place the guide hose 9 of the test assembly and adjust the up and down height position, for example, when placing, the guide hose 9 can be wrapped around the outside of the rotating guide roller 16 to reduce the space occupied by the guide hose 9; and when it is necessary to adjust the operating angle position of the injection needle 11 connected to the end of the guide hose 9, the electric push rod 17 is started, and the electric push rod 17 outputs kinetic energy to push the slide plate 13 to slide along the inside of the slide groove 14, thereby realizing the up and down height position adjustment of the mounting plate 15 installed on one side of the slide plate 13, so that the up and down height position of the guide hose 9 wrapped around the outside of the guide roller 16 is adjusted, and finally the inclination angle position of the injection needle 11 connected to the end of the guide hose 9 is changed; Compared with the traditional method that requires manual continuous holding of the diversion hose 9 and adjustment of the tilt angle of the injection needle 11, by installing the storage mechanism on the bracket 7, such operation steps can be omitted, saving time and effort; See also Figure 3In one embodiment of the present invention, the liquid supply mechanism includes a replacement fluid tank 21, a flow pump 24, a humidifier 25, a guide tube 23 and an air trap 28, wherein the replacement fluid tank 21 is used for temporarily storing and discharging the replacement fluid; the replacement fluid tank 21, the flow pump 24, the humidifier 25 and the air trap 28 are connected in sequence through the guide tube 23; a hydraulic pressure gauge 22 is installed at the position where the replacement fluid tank 21 is connected to the guide tube 23, and the installed hydraulic pressure gauge 22 is used to detect the hydraulic pressure of the replacement fluid discharged from the replacement fluid tank 21 in real time; the flow pump 24 is used to create a dynamic pressure inside the guide tube 23, so as to stably and continuously discharge the replacement fluid temporarily stored in the replacement fluid tank 21 in a quantitative manner; the humidifier 25 is used to humidify the discharged replacement fluid, and the installed air trap 28 is used to remove bubbles from the discharged replacement fluid; In the embodiment of the present invention, the humidifier 25 is used to humidify the replacement fluid for two purposes: first, to maintain the physiological function of the heart: the heart is an organ that is highly dependent on the temperature and humidity environment. In the experiment, in order to simulate the physiological environment in the body, it is necessary to maintain the appropriate humidity of the replacement fluid; the humidification treatment can ensure that the replacement fluid will not damage the heart tissue due to excessive dryness when entering the heart, thereby maintaining the normal physiological function of the heart; second, experimental accuracy: in the heart perfusion experiment, it is necessary to accurately measure and record various physiological parameters of the heart, such as heart rate, cardiac output, etc.; the humidification treatment can ensure the consistency of the experimental conditions, reduce the experimental errors caused by environmental factors, and thus improve the accuracy of the experiment; The degassing treatment of the replacement fluid by the air trap 28 also has multiple purposes: 1. Capture and remove bubbles: The main function of the air trap 28 is to capture and remove bubbles in the cardiac perfusate. During the cardiac perfusion process, if the perfusate contains bubbles, these bubbles may enter the heart and interfere with the normal function of the heart. The air trap 28 can effectively capture these bubbles through its special design, such as the conical structure, filter mesh, etc., and discharge them through the exhaust nozzle, thereby ensuring the purity and stability of the perfusate.

[0022] 2. Protect heart tissue: The presence of bubbles inside the heart may cause damage to the heart tissue. For example, bubbles may block the coronary arteries, resulting in insufficient blood supply to the heart; or when bubbles burst inside the heart, tiny shock waves may be generated, causing physical damage to the heart tissue. The use of the air trap 28 can effectively reduce these risks and protect the integrity and function of the heart tissue.

[0023] 3. Improve experimental accuracy: In the cardiac perfusion experiment, the accuracy of the experimental results is of vital importance to scientific researchers. The use of the gas trap 28 can ensure the purity and stability of the perfusion fluid, thereby eliminating the influence of bubbles on the experimental results. This helps to improve the accuracy and reliability of the experiment, allowing scientific researchers to more accurately evaluate the effects of factors such as drugs, temperature, and ions on cardiac function.

[0024] 4. Optimize experimental conditions: The air trap 28 not only helps to capture and remove bubbles, but also can optimize experimental conditions through its design. For example, some air traps 28 use a tangential blood inlet method, which increases the blood's travel in the trap, which is conducive to releasing bubbles entrained in the blood. This design can improve the efficiency of bubble capture while ensuring the smooth flow of perfusion fluid.

[0025] See also Figure 2 In an embodiment of the present invention, a control board 26 and a battery 27 are also installed inside the test bench 2. The control board 26 is used to control the normal operation of the entire liquid supply mechanism, and the installed battery 27 is used to provide kinetic energy to the entire liquid supply mechanism during operation.

[0026] See also Figure 4 In one embodiment of the present invention, a flow slot 30 is formed inside the flow pump 24, a liquid inlet 29 is opened on one side of the flow slot 30, and a liquid outlet 34 is opened on the other side of the flow slot 30; at the same time, a first flow tooth 31 and a second flow tooth 33 are symmetrically installed inside the flow slot 30, the outer side of the second flow tooth 33 is meshed with the outer side of the first flow tooth 31, and a driving shaft 32 of the motor is installed in the middle of the first flow tooth 31, and the motor is located outside the flow pump 24.

[0027] In an embodiment of the present invention, when the flow pump 24 is used to continuously, stably and quantitatively discharge the replacement fluid inside the replacement fluid tank 21, the motor is started, and the motor outputs kinetic energy to drive the first flow tooth 31 to rotate. Since the outer side of the first flow tooth 31 is meshed with the outer side of the second flow tooth 33, when the first flow tooth 31 rotates, the second flow tooth 33 is driven to rotate; and when the first flow tooth 31 and the second flow tooth 33 perform relative meshing motion, the replacement fluid entering the flow groove 30 will be stably, continuously and quantitatively discharged; by discharging the replacement fluid through this gear meshing, the discharge speed and flow rate of the replacement fluid can be stably controlled, and it can be stopped and started immediately.

[0028] See also Figure 5In the embodiment of the present invention, a filter chamber is formed inside the air collector 28, and the filter chamber is in the shape of a cross. A liquid guide port 40 is provided on one side of the filter chamber, and a liquid discharge port 41 is provided on the other side of the filter chamber. A filter assembly is installed inside the filter chamber, and the filter assembly includes a filter basket 38, an inclined filter plate 37, a connecting rod 36 and a sealing plug 35. The sealing plug 35 is installed on the outside of the air collector 28, and a connecting rod 36 is provided at the middle lower end of the humidifier 25. A inclined filter plate 37 is installed at the lower end of the connecting rod 36, and a filter basket 38 is fitted at the lower end of the inclined filter plate 37. At the same time, the lower end of the filter chamber is connected to a sewage tank 39.

[0029] In an embodiment of the present invention, when the replacement fluid enters the filter chamber from the position of the liquid guide port 40, the inclined filter plate 37 and the filter basket 38 cooperate with each other to filter, intercept and crush the bubbles contained in the replacement fluid entering, and the replacement fluid after bubble filtration is discharged from the position of the liquid discharge port 41; later, the inclined filter plate 37 and the filter basket 38 can be taken out from the position of the sealing plug 35 for cleaning; in addition, later, the waste liquid temporarily stored in the filter chamber can be cleaned and discharged in a centralized manner by taking out the sewage tank 39.

[0030] In the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An animal heart perfusion experimental device, comprising: A test bench (2), a shock-absorbing pad (1), and a bracket (7), wherein the test bench (2) is located at the upper end of the shock-absorbing pad (1); and the characteristics are: An operating table is formed at the upper end of the test bench (2), and a test animal (12) is clamped and fixed on the operating table by a clamp (18); a test component for guiding the replacement fluid discharged by the fluid supply mechanism and injecting it into the test animal (12) is provided on the outer side of the test bench (2); a bracket (7) is provided at the upper end of the shock-absorbing pad (1) at a position close to one side of the test bench (2), and a device for placing the test component and adjusting its height position is installed on the bracket (7); A guide plate (5) is installed between the outer periphery of the operating table and the inner periphery of the test bench (2), and filter holes (6) are provided on the surface of the guide plate (5); at the same time, a liquid collecting tank (19) is formed inside the test bench (2), and the formed liquid collecting tank (19) is funnel-shaped; the lower ends of the filter holes (6) provided on the surface of the guide plate (5) face the inside of the liquid collecting tank (19), and the bottom end of the liquid collecting tank (19) is connected to a collecting pipe (20); a waste liquid collecting box (3) is also movably installed inside the test bench (2), and a pull ring (4) is installed on the outer side of the waste liquid collecting box (3); The test bench (2) is internally equipped with a liquid supply mechanism for continuously, quantitatively and stably providing a bubble-free, humidified replacement fluid required for a clamped test animal (12) to perform a cardiac perfusion test; the liquid supply mechanism comprises a replacement fluid tank (21), a flow pump (24), a humidifier (25), a flow guide tube (23) and an air trap (28); the replacement fluid tank (21), the flow pump (24), the humidifier (25) and the air trap (28) are connected in sequence via the flow guide tube (23); the flow A flow groove (30) is formed inside the metering pump (24), a liquid inlet (29) is provided on one side of the flow groove (30), and a liquid outlet (34) is provided on the other side of the flow groove (30); at the same time, first flow teeth (31) and second flow teeth (33) are symmetrically installed inside the flow groove (30), the outer side of the second flow teeth (33) meshes with the outer side of the first flow teeth (31), and a drive shaft (32) of a motor is installed in the middle of the first flow teeth (31), and the motor is located outside the flow pump (24).

2. The animal heart perfusion experimental device according to claim 1, characterized in that: The test assembly comprises a flow guiding end (10), a flow guiding hose (9) and an injection needle (11); one end of the flow guiding hose (9) is provided with the flow guiding end (10), and the flow guiding end (10) is connected to a liquid supply mechanism installed inside the test bench (2); the other end of the flow guiding hose (9) is provided with an injection needle (11), and the flow guiding hose (9) is wound on the storage mechanism.

3. The animal heart perfusion experimental device according to claim 2, characterized in that: The storage mechanism comprises a mounting plate (15), a guide roller (16) and an electric push rod (17); two guide rollers (16) are movably mounted in a linear arrangement on the outer side of the mounting plate (15), and a guide hose (9) is wound around the outer side of the guide roller (16); a slide plate (13) is mounted on the inner side of the mounting plate (15); a lifting groove (8) is provided inside the bracket (7), and a slide groove (14) is provided on the inner wall of the lifting groove (8); the slide plate (13) mounted on the inner side of the mounting plate (15) is slidably mounted inside the slide groove (14) provided on the inner wall of the lifting groove (8); and the lower end of the slide plate (13) is connected to the output end of the electric push rod (17), and the electric push rod (17) is located inside the lifting groove (8).

4. The animal heart perfusion experimental device according to claim 1, characterized in that: A control panel (26) and a battery (27) are also installed inside the test bench (2).

5. The animal heart perfusion experimental device according to claim 1, characterized in that: A filter chamber is formed inside the air collector (28), and the filter chamber is in the shape of a cross. A liquid guide port (40) is provided on one side of the filter chamber, and a liquid discharge port (41) is provided on the other side of the filter chamber. A filter assembly is installed inside the filter chamber, and the filter assembly comprises a filter basket (38), an inclined filter plate (37), a connecting rod (36), and a sealing plug (35). The sealing plug (35) is installed outside the air collector (28). A connecting rod (36) is provided at the middle lower end of the humidifier (25). The inclined filter plate (37) is installed at the lower end of the connecting rod (36), and the filter basket (38) is fitted to the lower end of the inclined filter plate (37). Meanwhile, the lower end of the filter chamber is connected to a sewage discharge tank (39).

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