Application of ethylicin to prevention and treatment of sundries in epimedium plant planting
By using allicin mixed solution or allicin-complex fertilizer mixture to apply medicine to the genus Epimedium plants, the problem of difficult prevention and control in the existing technology is solved, and efficient prevention and control of the genus and healthy growth of epimedium seedlings is achieved.
Patent Information
- Application Number
- CN202510006385.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-02
- Publication Date
- 2025-04-11
AI Technical Summary
During the planting of Epimedium plants, the existing technology is difficult to effectively prevent and control the growth of Epimedium, resulting in long-term high humidity in the matrix and inhibit the growth of Epimedium seedlings.
Allicin mixed solution or allicin-complex fertilizer mixture is used to apply medicine to the genus Epimedium plants. By spraying it on the foliar surface or root of the genus, combined with appropriate matrix temperature and humidity conditions, the prevention and control of the genus is achieved.
It has achieved efficient prevention and control of land and money, with a prevention and control rate of more than 70%, avoiding the labor-consuming and time-consuming problem of manual removal, and at the same time, it has no obvious inhibition on the growth of epimedium seedlings, and even promoted its growth.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of agricultural drugs, and particularly relates to the use of ethionine in preventing and controlling foreign matter in the planting of Epimedium plants. Background Art
[0002] Epimedium brevicornum, also known as Xianlingpi, Sanzhi Jiuyecao, etc., is a plant of the genus Epimedium of the Berberidaceae family. It was first recorded in the "Shennong Bencao Jing" and has a long history of application. Its dried leaves are used as medicine, which has the effects of nourishing kidney yang, strengthening bones and tendons, and removing rheumatism. There are 40 species of Epimedium plants in China, of which 4 species are included in the "Chinese Pharmacopoeia" as Epimedium medicinal materials: Epimedium, Sagittae, Wollose Epimedium, and Korean Epimedium; in addition, Wushan Epimedium is included in the "Chinese Pharmacopoeia" as a single variety, and Qianling Epimedium and Coarse-haired Epimedium are included in the "Guizhou Provincial Traditional Chinese Medicine Standard". Epimedium is also a medicinal plant that can be used in health food approved by the Ministry of Health (now the National Health Commission) (No. 51 in 2002), and is a typical medicinal and edible plant resource. In recent years, innovative Chinese medicines with epimedium as the main raw material have made Epimedium plants highly valued and become one of the medicinal plants with the most development potential in China.
[0003] At present, several varieties such as sagittaria and pubescent epimedium have achieved large-scale seedling cultivation and planting. However, in the production of epimedium seedlings, due to the long-term maintenance of suitable temperature and high humidity, the growth of debris is often induced. The debris is mainly moss plants such as liverworts. Among them, liverworts have fast reproduction speed, fast growth speed, large growth volume, and are very easy to cover the surface of the substrate, causing the substrate to be in a high-humidity state for a long time; the rhizoids of liverworts are attached and inserted into the substrate and compete with epimedium for nutrients with the thallus structure covering the surface of the substrate. If prevention and control are not timely, it will soon break out on a large scale and inhibit the growth of epimedium seedlings, reducing the quality of seedlings. It has been observed that in the seedling tray full of liverworts, epimedium plants are short and have few leaves. Therefore, in the production of epimedium seedlings, a method for efficiently and quickly removing liverworts is urgently needed to improve the quality of seedlings and achieve efficient seedling cultivation. Summary of the invention
[0004] The problems existing in the prior art are: in the prior art, the method for preventing and controlling liverwort during the cultivation of Epimedium plants is usually spraying Bordeaux mixture, but it is time-consuming, requires many sprays, has low prevention effect, and also inhibits the growth of Epimedium seedlings; manually pulling out liverwort is time-consuming and labor-intensive, and difficult to pull out completely.
[0005] In view of the above problems existing in the prior art, the present invention provides ethionine for preventing and controlling debris in the cultivation of Epimedium plants.
[0006] Specifically, the present invention provides the following technical solutions:
[0007] In a first aspect, the present invention provides an application of ethionine, wherein ethionine is used for preventing and controlling debris in plant planting.
[0008] Preferably, the application is: ethionine is used for preventing and controlling debris in the cultivation of Epimedium plants.
[0009] Preferably, the debris is a moss plant, preferably, the moss plant is a liverwort.
[0010] Preferably, the ethionin emulsifiable concentrate product is formulated into a mixed solution for the prevention and treatment of liverwort, wherein the ethionin mixed solution is prepared by a method comprising the following steps: mixing components including the ethionin emulsifiable concentrate product and water,
[0011] More preferably, the content of ethionine in the ethionine emulsifiable concentrate product is 75wt-85wt%,
[0012] Most preferably, the content of ethionol is 80 wt%.
[0013] Preferably, the volume ratio of the ethionol emulsifiable concentrate product to water is: 0.5-2:998-999.5, preferably, the volume ratio of the ethionol emulsifiable concentrate product to water is: 0.67-2:998-999.33.
[0014] Preferably, the method further comprises using the mixture of the ethionine and compound fertilizer for the prevention and treatment of liverwort in Epimedium plants, wherein the mixture of the ethionine and compound fertilizer is prepared by a method comprising the following steps: mixing the ethionine emulsifiable concentrate product and the compound fertilizer solution in a volume ratio of 1-3:997-999 to obtain the mixture of the ethionine and compound fertilizer, wherein the compound fertilizer solution comprises urea, potassium dihydrogen phosphate and water.
[0015] Preferably, each liter of the compound fertilizer solution contains 1-5 g of urea, and / or each liter of the compound fertilizer solution contains 1-5 g of potassium dihydrogen phosphate.
[0016] Preferably, the plant of the genus Epimedium includes one or more substances selected from the group consisting of Epimedium, Epimedium sagittatum, Epimedium pubescens and Epimedium koreana.
[0017] More preferably, the plant of the genus Epimedium is one or a combination of two of Epimedium sagittatum and Epimedium pubescens.
[0018] Most preferably, the plant of the genus Epimedium is Epimedium sagittatum or Epimedium pubescentis.
[0019] Preferably, the method comprises the following steps: applying the ethiocarb mixed solution to Marchantia polyphylla in the genus Epimedium to achieve the prevention and treatment of Marchantia polyphylla.
[0020] Preferably, the method comprises the following steps:
[0021] (1) For the first control, use the mixture of ethoxylated chloranthide to apply pesticides to the Marchantia polyphylla in the Epimedium genus. Spray it on the surface or root of the Marchantia polyphylla until the mixture drips down from the surface of the Marchantia polyphylla. Then, spraying is completed. Spraying and watering of the Epimedium genus plants is prohibited within 1 day after application.
[0022] (2) within 15-30 days after applying the pesticide according to step (1), observe whether there is a recurrence of Marchantia polyphylla. If there is a recurrence of Marchantia polyphylla, apply the mixture of ethyl allicin or ethyl allicin-compound fertilizer to the Marchantia polyphylla of the genus Epimedium. After applying the pesticide, continue to observe for more than 15 days, preferably 15-30 days. If a recurrence of Marchantia polyphylla is observed, repeat the application of the pesticide according to step (2) once. The recurrence of Marchantia polyphylla refers to the appearance of new Marchantia polyphylla thallus structures on the substrate surface of the seedling tray, which is considered a recurrence.
[0023] (3) After applying the pesticide according to the above step (1) or (2), continue to observe for 15-30 days. If there is no recurrence of Marchantia odorata, use the compound fertilizer solution to apply the pesticide to the Marchantia odorata of the genus Epimedium to complete the prevention and control of Marchantia odorata.
[0024] Preferably, in step (1), the method of applying the pesticide is spraying.
[0025] And / or in step (1), the surface of the substrate of the seedling tray is covered with Marchantia polyphylla and Epimedium plant seedlings, wherein the area of Marchantia polyphylla in the seedling tray accounts for greater than or equal to 90% and less than 100%;
[0026] And / or in step (1), the dosage of the pesticide is: spraying the surface of the fronds of Marchantia polyphylla until the mixed liquid on the surface of the fronds of Marchantia polyphylla drops down, and then the spraying is completed.
[0027] Preferably, in step (2), the sign of completing the control of liverwort is that: after applying the compound fertilizer solution or the mixture of ethionine and compound fertilizer until the number of leaves of the Epimedium plant is greater than or equal to 3, and / or the maximum leaf length is greater than or equal to 1 cm, and / or the maximum leaf width is greater than or equal to 1 cm, preferably, the number of leaves is 3-10, and / or the maximum leaf length is 1-10 cm, and / or the maximum leaf width is 1-10 cm, more preferably, the number of leaves is 3-8, and / or the maximum leaf length is 1-5 cm, and / or the maximum leaf width is 1-5 cm;
[0028] And / or in step (2), the dosage of the pesticide is: spraying the thallus surface of the Marchantia polyphylla and / or the leaf surface of the Epimedium plant seedlings until water flows out from the bottom of the matrix of the seedling tray, so that the thallus surface of the Marchantia polyphylla and / or the roots and leaf surface of the Epimedium plant seedlings have been sprayed.
[0029] Preferably, during the application of pesticides, the substrate temperature of the seedling tray is 19-25°C, and the substrate relative humidity of the seedling tray is 60-65%.
[0030] Beneficial effects of the present invention:
[0031] The prepared ethionine mixed solution of the present invention is used to prevent and control liverworts, with a control rate of more than 70%. It has obvious selectivity for liverworts, fast action, and good control effect, and solves the problem of manual removal of liverworts in the process of cultivating epimedium plants in the seedling stage, which leads to labor and time consumption. In addition, the application of the ethionine mixed solution has no obvious growth inhibition on the seedlings of epimedium plants, and the combined application of the ethionine-compound fertilizer mixed solution or the compound fertilizer solution can further promote the growth of the seedlings, which has positive significance for the large-scale seedling cultivation of epimedium plants. BRIEF DESCRIPTION OF THE DRAWINGS
[0032] Figure 1 Shown are the growth status of Marchantia polymorpha and Epimedium pubescens seedlings in the seedling trays on the 1st, 2nd, 5th, 6th, 7th, 15th, 30th and 50th day after application of ethoxylated solutions 1-5, as well as the growth status of Marchantia polymorpha and Epimedium pubescens seedlings in the seedling trays of the blank control group and the manual removal group.
[0033] Figure 2 This is a diagram showing the status of the leaflets of Marchantia polyphylla 7 days after application of ethoxylated solution 1.
[0034] Figure 3 The changes in the number of leaves of Epimedium pubescens and the growth inhibition rate of the number of leaves in Examples 2.1-2.5, wherein: Figure 3 The growth inhibition rate was expressed as the growth inhibition rate per leaf number.
[0035] Figure 4 The maximum leaf length and the growth inhibition rate of the maximum leaf length of Epimedium pubescens in Examples 2.1-2.5 are shown in FIG. Figure 4 The median leaf length inhibition rate was expressed as the growth inhibition rate of the maximum leaf length.
[0036] Figure 5 The maximum leaf width and the growth inhibition rate of the maximum leaf width of Epimedium pubescens in Examples 2.1-2.5 are shown in FIG. Figure 4 The median leaf width inhibition rate was expressed as the growth inhibition rate of the maximum leaf width, Figure 4 The median growth inhibition rate was expressed as the growth inhibition rate of the maximum leaf width.
[0037] Figure 6 2.4, wherein A to I are growth status diagrams of Marchantia polyphylla and Epimedium pubescentis seedlings before application, 1 day, 2 days, 5 days, 6 days, 7 days, 15 days, 30 days and 50 days after application, respectively.
[0038] Figure 7The changes in the number of leaves of Epimedium pubescens and the growth inhibition rate of the number of leaves in Examples 3.1-3.5, wherein: Figure 7 The growth inhibition rate was expressed as the growth inhibition rate per leaf number.
[0039] Figure 8 The maximum leaf length and the growth inhibition rate of the maximum leaf length of Epimedium pubescens in Examples 3.1-3.5 are shown in FIG. Figure 8 The median leaf length inhibition rate was expressed as the growth inhibition rate of the maximum leaf length.
[0040] Fig. 9 The maximum leaf width and the growth inhibition rate of the maximum leaf width of Epimedium pubescens in Examples 3.1-3.5 are shown in FIG. Fig. 9 The median leaf width inhibition rate was expressed as the growth inhibition rate of the maximum leaf width, Fig. 9 The median growth inhibition rate was expressed as the growth inhibition rate of the maximum leaf width.
[0041] Fig.10 Shown are the growth status of the Epimedium sagittatum seedlings in the seedling tray of Example 3.4 before application of pesticides, 3 days, 5 days, 15 days, and 50 days after application of pesticides, and the growth status of the Marchantia polymorpha and Epimedium sagittatum seedlings in the seedling trays of the blank control group and the manual removal group. DETAILED DESCRIPTION
[0042] In order to better understand the above technical solution, the technical solution of the present invention is clearly and completely explained in conjunction with the specific implementation method below. It should be noted that the content in the specific implementation method is only a specific implementation and explanation of the technical solution of the present invention, and should not be understood as limiting the scope of protection of the present invention.
[0043] In some specific embodiments, the present invention provides an application of ethionine, wherein ethionine is used for the prevention and control of liverwort in Epimedium plant cultivation, wherein the prevention and control rate of liverwort is greater than or equal to 70%, preferably, the prevention and control rate of liverwort in the application may be 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100%, or or the prevention and control rate of liverwort within a numerical range consisting of any two of the above specific numerical values as endpoints.
[0044] In some specific embodiments, the present invention further provides a method for controlling liverwort in Epimedium plants, and the technical solution of the method is specifically as follows:
[0045] 1. A method for controlling Marchantia polyphylla in the cultivation of Epimedium plants, characterized in that it comprises the following steps: applying a mixed solution of ethionine to Marchantia polyphylla in Epimedium plants to complete the control of Marchantia polyphylla, wherein the mixed solution of ethionine is prepared by a method comprising the following steps: mixing components including an ethionine emulsifiable concentrate product and water.
[0046] 2. The method according to technical solution 1 is characterized in that the content of ethionine in the ethionine emulsifiable concentrate product is 75wt-85wt%, and more preferably, the content of ethionine in the ethionine emulsifiable concentrate product is 80wt%.
[0047] 3. The method according to technical solution 3 is characterized in that the volume ratio of the ethionine emulsifiable concentrate product to water is: 0.5-2:998-999.5, preferably, the volume ratio of the ethionine emulsifiable concentrate product to water is: 0.67-2:998-999.33.
[0048] 4. The method according to technical solution 3 or 4 is characterized in that it also includes using the mixed solution of ethionine-compound fertilizer for the prevention and control of liverwort in Epimedium plants, wherein the mixed solution of ethionine-compound fertilizer is prepared by a method comprising the following steps: mixing the ethionine emulsifiable concentrate product and the compound fertilizer solution in a volume ratio of 1-3:997-999 to obtain the mixed solution of ethionine-compound fertilizer, wherein the compound fertilizer solution includes urea, potassium dihydrogen phosphate and water.
[0049] 5. The method according to technical solution 5 is characterized in that each liter of compound fertilizer solution contains 1-5 g of urea, and / or each liter of compound fertilizer solution contains 1-5 g of potassium dihydrogen phosphate.
[0050] 6. The method according to any one of technical solutions 1 to 5, characterized in that it comprises the following steps:
[0051] (1) For the first control, use the ethiocarb mixture to apply pesticides to the liverworts of the Epimedium genus. Spray the mixture on the surface of the leaf blades or roots of the liverworts until the mixture drips down from the leaf blades of the liverworts. Then, spraying and watering the Epimedium genus plants is prohibited within 1 day after the application.
[0052] (2) Within 15-30 days after applying the pesticide according to step (1), observe whether there is a recurrence of the Marchantia polyphylla. If there is a recurrence of the Marchantia polyphylla, apply the mixture of ethyl allicin or ethyl allicin-compound fertilizer to the Marchantia polyphylla of the genus Epimedium. After continuous observation for more than 15 days after the application of the pesticide, observe again whether there is a recurrence of the Marchantia polyphylla. If there is a recurrence of the Marchantia polyphylla, repeat the application of the pesticide according to step (2) once.
[0053] Among them, the phenomenon of liverwort relapse refers to the appearance of new liverwort fronds on the substrate surface of the seedling tray, which is considered a relapse;
[0054] (3) After applying the pesticide according to the above steps (1) and / or (2), observation is continued for 15-30 days. If recurrence of Marchantia milii is observed, the compound fertilizer solution is used to apply pesticide to Marchantia milii in the genus Epimedium to complete the prevention and control of Marchantia milii.
[0055] 7. The method according to any one of technical solutions 1 to 6, characterized in that:
[0056] In step (1), the method of applying the pesticide is: spraying;
[0057] And / or in step (1), the surface of the substrate of the seedling tray is covered with Marchantia polyphylla and Epimedium plant seedlings, wherein the area of Marchantia polyphylla in the seedling tray accounts for greater than or equal to 90% and less than 100%;
[0058] And / or in step (1), the dosage of the pesticide is: spraying the surface of the fronds of Marchantia polyphylla until the mixed liquid on the surface of the fronds of Marchantia polyphylla drops down, and then the spraying is completed.
[0059] 8. The method according to any one of technical solutions 1 to 7 is characterized in that in step (2), the sign of completing the control of liverwort is that after applying the fertilizer solution or the mixture of ethyl allicin and compound fertilizer to the Epimedium plant, the number of leaves is greater than or equal to 3, and / or the maximum leaf length is greater than or equal to 1 cm, and / or the maximum leaf width is greater than or equal to 1 cm, preferably, the number of leaves is 3-10, and / or the maximum leaf length is 1-10 cm, and / or the maximum leaf width is 1-10 cm, more preferably, the number of leaves is 3-8, and / or the maximum leaf length is 1-5 cm, and / or the maximum leaf width is 1-5 cm.
[0060] And / or in step (2), the dosage of the pesticide is: spraying the thallus surface of the Marchantia polyphylla and / or the leaf surface of the Epimedium plant seedlings until water flows out from the bottom of the matrix of the seedling tray, so that the thallus surface of the Marchantia polyphylla and / or the roots and leaf surface of the Epimedium plant seedlings have been sprayed.
[0061] 9. The method according to any one of technical solutions 1 to 8 is characterized in that during the application of the pesticide, the substrate temperature of the seedling tray is 19-25°C and the relative humidity of the substrate of the seedling tray is 60-65%.
[0062] 10. The method according to any one of technical solutions 1 to 9, characterized in that the control rate of liverwort is greater than or equal to 70%, preferably, the control rate of liverwort is 70-90%,
[0063] And / or the damage rate of Epimedium plants is 5%-9.80%, preferably, the damage rate of Epimedium plants is 6%-8%.
[0064] 11. The method according to any one of technical solutions 1 to 10, characterized in that in step (1), after the Epimedium plant containing liverwort is treated with the ethoxylated mixed solution, the growth inhibition rate of the number of leaves of the Epimedium plant is less than 23.5%, and / or the growth inhibition rate of the maximum leaf length of the Epimedium plant is less than 12.5%, and / or the growth inhibition rate of the widest leaf length of the Epimedium plant is less than 10%,
[0065] Preferably, the growth inhibition rate for the number of leaves of Epimedium plants is greater than -20% and less than 23.5%, and / or the growth inhibition rate for the maximum leaf length of Epimedium plants is greater than -35% and less than 12.5%, and / or the growth inhibition rate for the widest leaf length of Epimedium plants is greater than -35% and less than 10%.
[0066] 12. The method according to any one of technical solutions 1 to 11 is characterized in that, in step (2), after the Epimedium plant containing liverwort is treated with a solution containing compound fertilizer or a solution containing ethionine-compound fertilizer, the growth inhibition rate of the number of leaves of the Epimedium plant is less than 3%, and / or the growth inhibition rate of the maximum leaf length of the Epimedium plant is less than 2%, and / or the growth inhibition rate of the widest leaf length of the Epimedium plant is less than 3%.
[0067] Preferably, the growth inhibition rate for the number of leaves of Epimedium plants is greater than -25% and less than 3%, and / or the growth inhibition rate for the maximum leaf length of Epimedium plants is greater than -20% and less than 2%, and / or the growth inhibition rate for the widest leaf length of Epimedium plants is greater than -25% and less than 0%.
[0068] 13. The method according to any one of technical solutions 1 to 12 is characterized in that the Epimedium plant includes one or more substances selected from the group consisting of Epimedium, Epimedium sagittatum, Epimedium pubescens and Epimedium koreana.
[0069] 14. The method according to any one of technical solutions 1 to 13, characterized in that the plant of the genus Epimedium is one or a combination of two of Epimedium sagittatum and Epimedium pubescens.
[0070] Preferably, the plant of the genus Epimedium is one or a combination of Epimedium sagittatum and Epimedium pubescens.
[0071] More preferably, the plant of the genus Epimedium is Epimedium sagittatum or Epimedium pubescentis.
[0072] In order to better understand the technical solution of the present invention, the technical solution of the present invention is described in detail below in conjunction with a specific ethionine solution. The various reagents / instruments used in the ethionine solution of the present invention are conventional commercial products unless otherwise specified. The sources of information on the experimental reagents and experimental instruments used in the present invention are shown in Table 1.
[0073] Table 1. Experimental reagents / instrument information
[0074]
[0075] Example 1
[0076] (1) Preparation of ethyl garlic solution with different ethyl garlic content:
[0077] Ethyl garlic solution 1: Mix 2 mL of ethyl garlic emulsifiable concentrate (with an ethyl garlic content of 80 wt%) with 998 mL of water to obtain ethyl garlic solution 1.
[0078] Ethyl garlic solution 2: 1.33 mL of ethyl garlic emulsifiable concentrate (the content of ethyl garlic is 80 wt%) was mixed with 998.67 mL of water to obtain ethyl garlic solution 2.
[0079] Ethiocarb solution 3: 1 mL of ethiocarb emulsifiable concentrate (the content of ethiocarb is 80 wt%) was mixed with 999 mL of water to obtain ethiocarb solution 3.
[0080] Ethiocarb solution 4: 0.8 mL of ethiocarb emulsifiable concentrate (the content of ethiocarb is 80 wt%) was mixed with 999.2 mL of water to obtain ethiocarb solution 4.
[0081] Ethiocarb solution 5: 0.67 mL of ethiocarb emulsifiable concentrate (the content of ethiocarb is 80 wt%) was mixed with 999.33 mL of water to obtain ethiocarb solution 5.
[0082] (2) Spraying the ethionine solution 1, ethionine solution 2, ethionine solution 3, ethionine solution 4 and ethionine solution 5 obtained in step (1) on corresponding seedling trays in a greenhouse, respectively, to prevent and control the liverwort in the seedling trays; the area of the seedling trays is 27 cm×53 cm, and the liverwort covers the surface of the seedling trays, i.e., at the base of the epimedium seedlings. Select a seedling tray covered with liverwort, i.e., the liverwort leaf-like structure covers the surface of the seedling tray, and the epimedium seedlings have two true leaves (belonging to the seedling stage).
[0083] The spraying method of the ethionine solution is: spray the ethionine solution on the Marchantia polyphylla in the seedling tray, spray it on the surface of the leaf blades or the roots of the Marchantia polyphylla, until the ethionine solution on the surface of the leaf blades of the Marchantia polyphylla drips down, and the spraying is completed. It is forbidden to spray or water the Epimedium plants within 1 day after application.
[0084] It should be noted that during the application of pesticides, the substrate temperature in the greenhouse was 19-25°C, the substrate relative humidity in the greenhouse was 60%, and there was no strong wind in the greenhouse.
[0085] Among them, the seedling tray sprayed with clean water was used as the blank control group; the seedling tray with manual removal of liverwort was used as the manual removal group. Three parallel groups were set up in each group. The coverage area and growth of Epimedium pubescens seedlings and liverwort in each seedling tray were similar.
[0086] (3) Taking photos to record the growth status of the Marchantia polymorpha and Epimedium pubescens seedlings in the corresponding seedling trays on the 1st day, 2nd day, 5th day, 6th day, 7th day, 15th day, 30th day and 50th day after applying ethoxylated solution 1 to ethoxylated solution 5, as well as the growth status of the Marchantia polymorpha and Epimedium pubescens seedlings in the seedling trays of the blank control group and the manual removal group. The results are as follows: Figure 1 Shown and Figure 2 shown.
[0087] like Figure 1 As shown, Figure 1 The graphs show the growth status of Marchantia polymorpha and Epimedium pubescens seedlings in the seedling trays on the 1st day, 2nd day, 5th day, 6th day, 7th day, 15th day, 30th day and 50th day after applying ethiocarb solution 1-5, and the growth status of Marchantia polymorpha and Epimedium pubescens seedlings in the seedling trays of the blank control group and the manual removal group. Figure 1 As shown, from left to right, each column represents the manual removal group, blank control group, ethyl allicin solution 1, ethyl allicin solution 2, ethyl allicin solution 3, ethyl allicin solution 4, ethyl allicin solution 5; Figure 1 The (A) row in the figure shows the growth status of Marchantia polyphylla and Epimedium pubescens seedlings that were not manually pulled out or not applied with ethyl allicin solution; Figure 1 In row (B), Figure 1 In the (C) row, Figure 1 In the row (D), Figure 1 In the row (E), Figure 1 In the (F) row, Figure 1 In the (G) row, Figure 1 The (H) row and Figure 1 The (I) row in the figure represents the growth status of Marchantia polyphylla and Epimedium pubescentis seedlings on the 1st, 2nd, 5th, 6th, 7th, 15th, 30th and 50th day after the application of ethyl allicin solution.
[0088] The results showed that Figure 1From the (A) row in the figure, we can see that the initial proportion of Marchantia polyphylla is greater than or equal to 90% and less than 100%. On the first day after the application of ethionin solution 1, ethionin solution 2, ethionin solution 3, ethionin solution 4 or ethionin solution 5, the leaf structure of Marchantia polyphylla showed obvious symptoms, and began to change color, turn green and yellow, and wilt; as the active ingredient ethionin continued to work in the body of Marchantia polyphylla, the leaf structure turned yellow and dried up over a large area. Figure 1 As shown in row (F) in the figure, the fronds of Marchantia polyphylla were completely dead 7 days after application of ethionin solution 1, ethionin solution 2, ethionin solution 3, ethionin solution 4 or ethionin solution 5.
[0089] like Figure 2 As shown, Figure 2 This is a diagram showing the status of the leaflets of Marchantia polyphylla 7 days after application of ethoxylated solution 1. Figure 2 The middle arrow points to the reproductive structure of the liverwort. The results show that the dead leaves can be easily pulled out after being treated with ethyl allicin solution 1 for 7 days. The dead leaves can be used as nutrients for the epimedium seedlings after being moistened and rotted on the surface of the substrate by watering, and the reproductive structure of the liverwort, namely the male and female receptacles, can be easily pulled out.
[0090] (2) Samples of Marchantia polyphylla were collected from three locations in the seedling trays of the blank control group and the manual removal group 15 days after the application of ethoxylated solution 1 to ethoxylated solution 5, and from three locations in the seedling trays of the blank control group and the manual removal group. All the Marchantia polyphylla in the sampling points were pulled out and their fresh weights were weighed. The fresh weight control rate of Marchantia polyphylla was calculated based on the fresh weights of the Marchantia polyphylla. The calculation formula is shown in Formula 1, and the calculation results are shown in Table 2.
[0091] Fresh weight control rate of Marchantia solani = (fresh weight of Marchantia solani in the manual removal group - fresh weight of Marchantia solani applied with ethyl allicin solution) / fresh weight of Marchantia solani in the manual removal group × 100% Formula 1.
[0092] (3) The number of leaves of the Epimedium pubescent seedlings in the seedling trays 15 days after the application of ethoxylated solutions 1-5, as well as in the seedling trays of the blank control group and the manual removal group were counted, and the leaf damage rate of the Epimedium pubescent seedlings was calculated. The calculation formula is shown in Formula 2, and the calculation results are shown in Table 2.
[0093] Leaf damage rate of Epimedium pubescens seedlings = number of leaves damaged by phytosanitizing agents / total number of leaves × 100% Formula 2 Table 2 Results of fresh weight control rate of Marchantia polyphylla and leaf damage rate of Epimedium pubescens seedlings 15 days after application of ethoxylated solution 1-5
[0094]
[0095] Note: Different lowercase letters after the data in the same column indicate significant differences (P<0.05).
[0096] As shown in Table 2, the results showed that after the 15th day of applying ethionin solution 1 to ethionin solution 5, ethionin solution 1 to ethionin solution 5 showed obvious selectivity to liverwort, among which, the fresh weight control rate of liverwort by applying ethionin solution 1, ethionin solution 2, ethionin solution 3 and ethionin solution 4 was above 80% and there was no significant difference. There was no significant difference in the leaf damage rate of Epimedium pubescens seedlings by applying ethionin solution 3, ethionin solution 4 and ethionin solution 5. However, the leaf damage rate of Epimedium seedlings by applying ethionin solution 1 was as high as 32.2%, and the leaf damage rate of Epimedium seedlings by applying ethionin solution 2 was 19.08%. The leaf damage rate of Epimedium seedlings by ethionin solution 1 and ethionin solution 2 was significantly different from that of ethionin solution 3, ethionin solution 4 and ethionin solution 5.
[0097] Example 2
[0098] Example 2.1 provides a method for promoting the growth of Epimedium pubescens seedlings, and the specific steps of the method are as follows:
[0099] (1) Spray the ethoxylated solution 1 on the corresponding seedling trays in the greenhouse to apply the pesticide to the Epimedium pubescens seedlings and Marchantia polyphylla in the seedling trays for 30 days; at the same time, the seedling trays without spraying the ethoxylated solution and without manual removal of Marchantia polyphylla were used as the blank control group; the seedling trays with manual removal of Marchantia polyphylla were used as the manual removal group. Three parallel groups were set up in each group, in which the coverage area and growth of the Epimedium pubescens seedlings and Marchantia polyphylla in the seedling trays were similar. The area of the seedling tray was 27 cm × 53 cm, and the Marchantia polyphylla covered the surface of the seedling tray, that is, at the base of the Epimedium pubescens seedlings. The seedling trays covered with Marchantia polyphylla were selected, that is, the Marchantia polyphylla leaf-like structure covered the surface of the seedling tray, and the Epimedium pubescens seedlings had two true leaves (belonging to the seedling stage).
[0100] The spraying method of the ethionine solution is: spray the ethionine solution on the Marchantia polyphylla in the seedling tray, spray it on the surface of the leaf blades or the roots of the Marchantia polyphylla, until the ethionine solution on the surface of the leaf blades of the Marchantia polyphylla drips down, and the spraying is completed. It is forbidden to spray or water the Epimedium plants within 1 day after application.
[0101] It should be noted that during the application of pesticides, the substrate temperature in the greenhouse was 19-25°C, the substrate relative humidity in the greenhouse was 60%, and there was no strong wind in the greenhouse.
[0102] (2) After the 30th day of applying the ethionine solution 1, the liverwort relapsed after 20 days of continuous observation. Then the ethionine-compound fertilizer solution was applied until the 50th day (i.e., the ethionine-compound fertilizer solution was applied for 20 days).
[0103] The preparation method of the ethionine-compound fertilizer solution is as follows: 2 mL of ethionine emulsifiable concentrate and 998 mL of compound fertilizer solution are mixed to obtain the ethionine-compound fertilizer solution. The preparation method of the compound fertilizer solution is as follows: 3 g of urea, 3 g of potassium dihydrogen phosphate and water are mixed and the volume is adjusted to 1000 mL to obtain the compound fertilizer solution.
[0104] It should be noted that in step (2), after the 30th day of applying the ethiocarb solution 1, the observation was continued for 20 days. If the recurrence of Marchantia polyphylla was observed, the ethiocarb solution 1 was used again once. The recurrence of Marchantia polyphylla refers to the appearance of new Marchantia polyphylla thallus structures on the surface of the matrix, which is considered to be a recurrence.
[0105] If there is no recurrence of Marchantia odorata, the compound fertilizer solution is applied until the 50th day (i.e., the compound fertilizer solution is applied for 20 days), wherein the preparation method of the compound fertilizer solution is: 3g urea, 3g potassium dihydrogen phosphate and water are mixed and the volume is fixed to 1000mL to obtain the compound fertilizer solution;
[0106] If there is a recurrence of liverwort, apply the ethyl allicin-compound fertilizer solution until the 50th day (i.e. apply the ethyl allicin-compound fertilizer solution for 20 days).
[0107] The spraying method of ethoxylated-compound fertilizer solution or compound fertilizer solution is: spray the surface of the seedling tray until water flows out from the bottom of the substrate of the seedling tray, and the spraying is completed. If water flows out from the bottom of the substrate of the seedling tray, it means that the roots and leaves of Epimedium have been sprayed.
[0108] Example 2.2 provides a method for promoting the growth of Epimedium pubescens seedlings, and the specific steps of the method are as follows: the difference from Example 2.1 is that the ethionine solution 1 is replaced by the ethionine solution 2. Example 2.2 shows the phenomenon of Marchantia polyphylla recurrence.
[0109] Example 2.3 provides a method for promoting the growth of Epimedium pubescens seedlings, and the specific steps of the method are as follows: the difference from Example 2.1 is that the ethionine solution 1 is replaced by the ethionine solution 3. Example 2.3 shows the phenomenon of Marchantia polyphylla recurrence.
[0110] Example 2.4 provides a method for promoting the growth of Epimedium pubescens seedlings, and the specific steps of the method are as follows: the difference from Example 2.1 is that the ethionine solution 1 is replaced by the ethionine solution 4. Example 2.4 shows the phenomenon of Marchantia polyphylla recurrence.
[0111] Example 2.5 provides a method for promoting the growth of Epimedium pubescens seedlings, and the specific steps of the method are as follows: the difference from Example 2.1 is that the ethionine solution 1 is replaced by the ethionine solution 5. Example 2.5 shows the phenomenon of recurrence of Marchantia polyphylla.
[0112] Technical effect evaluation
[0113] (1) After the 15th day of applying the ethionin solution, after the 30th day of applying the ethionin solution, and after the 50th day of applying the compound fertilizer solution or the ethionin-compound fertilizer solution (i.e., 20 days after applying the compound fertilizer solution or the ethionin-compound fertilizer solution), the number of leaves of the Epimedium pubescens seedlings, the maximum leaf length of the Epimedium pubescens seedlings, and the maximum leaf width of the Epimedium pubescens seedlings in Examples 2.1-2.5, the blank control group, and the manual removal group were recorded respectively. Among them, after the 15th day, the 30th day, and the 50th day, the number of leaves of the Epimedium pubescens seedlings, the maximum leaf length of the Epimedium pubescens seedlings, and the maximum leaf width of the Epimedium pubescens seedlings in the manual removal group were as shown in Table 3.
[0114] The leaf number inhibition rate, leaf length inhibition rate and leaf width inhibition rate of the Epimedium pubescentis seedlings in Examples 2.1-2.5 and the blank control group were calculated. The results are as follows: Figure 3 , Figure 4 and Figure 5 shown.
[0115] Growth inhibition rate of the number of leaves of Epimedium pubescens seedlings = (number of leaves of Epimedium pubescens seedlings in the manual removal group - number of leaves of Epimedium pubescens seedlings in the example) / number of leaves of Epimedium pubescens seedlings in the manual removal group × 100%. A growth inhibition rate of less than 10% means no significant phytotoxicity.
[0116] The growth inhibition rate of the maximum leaf length of Epimedium pubescens seedlings = (maximum leaf length of Epimedium pubescens seedlings in the manual removal group - maximum leaf length of Epimedium pubescens seedlings in the example) / maximum leaf length of Epimedium pubescens seedlings in the manual removal group × 100%. A growth inhibition rate of less than 10% means no significant phytotoxicity.
[0117] Growth inhibition rate of maximum leaf width of Epimedium pubescens seedlings = (maximum leaf width of Epimedium pubescens seedlings in manual removal group - maximum leaf width of Epimedium pubescens seedlings in Example) / maximum leaf width of Epimedium pubescens seedlings in manual removal group × 100%. When the growth inhibition rate is less than 10%, there is no significant phytotoxicity.
[0118] (2) Taking photos to record the growth status of the Epimedium pubescens seedlings in the seedling tray of the blank control group, as well as the growth status of the Marchantia polymorpha and Epimedium pubescens seedlings in the seedling tray of Example 2.4 after 1 day, 2 days, 5 days, 6 days, 7 days, 15 days, 30 days and 50 days. The results are as follows: Figure 6 Table 3. Number of leaves, maximum leaf length and maximum leaf width of Epimedium pubescens seedlings in the manual removal group after 15 days, 30 days and 50 days
[0119]
[0120] like Figure 3 , Figure 4 and Figure 5 As shown, Figure 3 The changes in the number of leaves of Epimedium pubescens and the growth inhibition rate of the leaves in Examples 2.1-2.5. Figure 4 The maximum leaf length and the growth inhibition rate of the maximum leaf length of Epimedium pubescens in Examples 2.1-2.5 are shown. Figure 5 The maximum leaf width and the growth inhibition rate of the maximum leaf width of Epimedium pubescens in Examples 2.1-2.5 are shown in Figure 2.1-2.5. The results show that the inhibition rate of Examples 2.1-2.5 on Marchantia polyphylla decreases over time, and the growth inhibition rate of the number of leaves and the leaf width is less than 10% at 30 days (it can be considered that there is no inhibitory effect on the growth of Epimedium pubescens seedlings, that is, it is safe for Epimedium pubescens); when the ethyl allicin-compound fertilizer solution is applied to the 50th day, the growth inhibition rates of the number of leaves, the maximum leaf length, and the leaf width of the treatment group are all less than or equal to 0, and it can be considered that this method can promote the growth of Epimedium pubescens. In the blank control group, the inhibition rate of the maximum leaf length and leaf width increased with time, and the inhibition rate of the leaf number was in a trend of first increasing and then decreasing, but the decreasing trend was slow; at 15 days, the blank control group had a significant inhibitory effect on the growth of the number of leaves of the seedlings, and its inhibition rate reached 29.79%, which was greater than that of the treatment group; 30 days after the application of the pesticide, the blank control group had a significant inhibitory effect on the number of leaves, leaf length and leaf width of the seedlings (the growth inhibition rate was greater than 20%). It can be seen that with the growth of the liverwort, the stronger the inhibitory effect of the liverwort on the leaf length and leaf width of the epimedium seedlings, the stronger the inhibitory effect of the liverwort on the leaf length and leaf width of the epimedium seedlings, and the more obvious the inhibitory effect on the growth of the leaf number. After 50 days of this method, the average number of leaves of the seedlings in the treatment group was more than 6, the average maximum leaf length was more than 4.34cm, and the maximum leaf width was more than 3.80cm.
[0121] like Figure 6 As shown, Figure 6 The growth status of the Marchantia polyphylla and Epimedium pubescens seedlings in the seedling tray of Example 2.4, wherein A to I are the growth status of the Marchantia polyphylla and seedlings before application, 1 day, 2 days, 5 days, 6 days, 7 days, 15 days, 30 days and 50 days later. The results showed that spraying the compound fertilizer solution 30 days after applying the ethoxylated solution 4 had a good control effect on the Marchantia polyphylla and promoted the good growth of the Epimedium pubescens seedlings.
[0122] Example 3
[0123] Example 3.1 provides a method for promoting the growth of Epimedium sagittatum seedlings, and the specific steps of the method are as follows: The difference from Example 2.1 is that the Epimedium pubescentis seedlings are replaced with Epimedium sagittatum seedlings. Example 3.1 has a recurrence phenomenon of Marchantia odorata.
[0124] Example 3.2 provides a method for promoting the growth of Epimedium sagittatum seedlings, and the specific steps of the method are as follows: The difference from Example 2.1 is that the Epimedium pubescens seedlings are replaced with Epimedium sagittatum seedlings. Example 3.2 shows the phenomenon of recurrence of liverwort.
[0125] Example 3.3 provides a method for promoting the growth of Epimedium sagittatum seedlings, and the specific steps of the method are as follows: the difference from Example 2.1 is that the Epimedium pubescens seedlings are replaced with Epimedium sagittatum seedlings. Example 3.3 shows the phenomenon of recurrence of liverwort.
[0126] Example 3.4 provides a method for promoting the growth of Epimedium sagittatum seedlings, and the specific steps of the method are as follows: The difference from Example 2.1 is that the Epimedium pubescens seedlings are replaced with Epimedium sagittatum seedlings. Example 3.4 shows the phenomenon of recurrence of Marchantia odorata.
[0127] Example 3.5 provides a method for promoting the growth of Epimedium sagittatum seedlings, and the specific steps of the method are as follows: the difference from Example 2.1 is that the Epimedium pubescens seedlings are replaced with Epimedium sagittatum seedlings. Example 3.5 is about the recurrence of Marchantia odorata.
[0128] Technical effect evaluation
[0129] (1) After the 15th day of applying the ethyl allicin solution, after the 30th day of applying the ethyl allicin solution, and after the 50th day of applying the compound fertilizer solution or the ethyl allicin-compound fertilizer solution (i.e., 20 days after applying the compound fertilizer solution or the ethyl allicin-compound fertilizer solution), the number of leaves, the maximum leaf length of the sagittal leaf Epimedium seedlings, and the maximum leaf width of the sagittal leaf Epimedium seedlings of Examples 3.1-3.5, the blank control group, and the manual removal group were recorded respectively. Among them, after the 15th day, the 30th day, and the 50th day, the number of leaves, the maximum leaf length, and the maximum leaf width of the sagittal leaf Epimedium seedlings of the manual removal group were as shown in Table 4.
[0130] The growth inhibition rates of the number of leaves of the sagittaria seedlings of Examples 3.1-3.5 and the blank control group, the growth inhibition rates of the maximum leaf length of the sagittaria seedlings, and the growth inhibition rates of the maximum leaf width of the sagittaria seedlings were calculated. The results are as follows: Figure 7 , Figure 8 and Fig. 9 shown.
[0131] The growth inhibition rate of leaves of E. sagitta seedlings = (the number of leaves of E. sagitta seedlings in the manual removal group - the number of leaves of E. sagitta seedlings in the embodiment) / the number of leaves of E. sagitta seedlings in the manual removal group × 100%. When the inhibition rate is less than 10%, there is no significant phytotoxicity.
[0132] The growth inhibition rate of the maximum leaf length of the Epimedium sagittatum seedlings = (maximum leaf length of the Epimedium sagittatum seedlings in the manual removal group - maximum leaf length of the Epimedium sagittatum seedlings in the embodiment) / maximum leaf length of the Epimedium sagittatum seedlings in the manual removal group × 100%. An inhibition rate of less than 10% means no significant phytotoxicity.
[0133] The growth inhibition rate of the maximum leaf width of the sagittium serrata seedlings = (maximum leaf width of the sagittium serrata seedlings in the manual removal group - maximum leaf width of the sagittium serrata seedlings in the example) / maximum leaf width of the sagittium serrata seedlings in the manual removal group × 100%. An inhibition rate of less than 10% means no significant phytotoxicity.
[0134] (2) Taking photos to record the growth status of the Epimedium sagittatum seedlings in the seedling tray of Example 3.4 before and after the application of pesticides on the 3rd, 5th, 15th and 50th days, and the growth status of the Epimedium sagittatum seedlings in the seedling tray of the blank control group and the manually removed group. The results are as follows: Fig.10 shown.
[0135] Table 4. Number of leaves, maximum leaf length and maximum leaf width of Epimedium sagittatum seedlings in the manual removal group after 15 days, 30 days and 50 days
[0136]
[0137] like Figure 7 , Figure 8 and Fig. 9 As shown, Figure 7 The changes in the number of leaves of Epimedium pubescens and the growth inhibition rate of the leaves in Examples 3.1-3.5. Figure 8 The changes in the maximum leaf length and maximum leaf length inhibition rate of Epimedium pubescens in Examples 3.1-3.5 are shown. Fig. 9 The maximum leaf width and the maximum leaf width growth inhibition rate of Epimedium pubescens in Examples 3.1-3.5 are shown in Table 1. The results show that 15 days after the application of Examples 3.1-3.5, the inhibition rates of the number of leaves, maximum leaf length and leaf width of Epimedium sagittatum in the treatment group and the blank control group were all less than 10%, and it can be considered that the treatment has no inhibitory effect on the growth of Epimedium sagittatum seedlings, that is, it is safe for Epimedium sagittatum.
[0138] like Fig.10 As shown, Fig.10The figures are the growth status of the Marchantia polymorpha and Epimedium sagittatum seedlings in the seedling tray of Example 3.4 without drug application, 3 days, 5 days, 15 days and 50 days after drug application, and the growth status of the Epimedium sagittatum seedlings in the seedling tray of the blank control group and the manually pulled out group, wherein row A is the blank control group, row B is Example 3.4, and row C is the manually pulled out group; and the first column is the growth status of the Epimedium sagittatum seedlings in the seedling tray without drug application, the second column is the growth status of the Epimedium sagittatum seedlings in the seedling tray 3 days after drug application, the third column is the growth status of the Epimedium sagittatum seedlings in the seedling tray 5 days after drug application, the fourth column is the growth status of the Epimedium sagittatum seedlings in the seedling tray 15 days after drug application, and the fifth column is the growth status of the Epimedium sagittatum seedlings in the seedling tray 50 days after drug application. The results showed that this method can effectively remove the liverwort in the Epimedium sagittatum seedling tray without affecting the growth of the Epimedium sagittatum seedlings.
[0139] The above embodiments are only for further explanation and understanding of the technical solution of the present invention, and are not limitations of the present invention. Any non-prominent substantial features and non-significant improvements made by those skilled in the art on this basis should fall within the scope of protection of the present invention.
Claims
1. An application of ethionine, characterized in that: The application is: ethionine is used for preventing and controlling debris in plant planting.
2. The use according to claim 1, characterized in that: The application is: ethionine is used for preventing and controlling debris in the planting of Epimedium plants.
3. The use according to claim 1 or 2, characterized in that: The sundries are bryophytes, preferably, the bryophytes are liverworts.
4. The use according to any one of claims 1 to 3, characterized in that: The ethionin emulsifiable concentrate product is formulated into a mixed solution for the prevention and treatment of liverwort, wherein the ethionin mixed solution is prepared by a method comprising the following steps: mixing components including the ethionin emulsifiable concentrate product and water, Preferably, the content of ethionine in the ethionine emulsifiable concentrate product is 75wt-85wt%, More preferably, the content of ethionol is 80 wt %.
5. The use according to claim 4, characterized in that: The volume ratio of the ethionyl acetate emulsifiable concentrate product to water is 0.5-2:998-999.
5. Preferably, the volume ratio of the ethionyl acetate emulsifiable concentrate product to water is 0.67-2:998-999.
33.
6. The use according to claim 4 or 5, characterized in that: The method also includes using the mixed solution of ethionine and compound fertilizer for the prevention and treatment of liverwort in Epimedium plants, wherein the mixed solution of ethionine and compound fertilizer is prepared by a method comprising the following steps: mixing the ethionine emulsifiable concentrate product and the compound fertilizer solution in a volume ratio of 1-3:997-999 to obtain the mixed solution of ethionine and compound fertilizer, wherein the compound fertilizer solution comprises urea, potassium dihydrogen phosphate and water.
7. The use according to claim 6, characterized in that: Each liter of the compound fertilizer solution contains 1-5 g of urea, and / or each liter of the compound fertilizer solution contains 1-5 g of potassium dihydrogen phosphate.
8. The use according to any one of claims 1 to 7, characterized in that: The plants of the genus Epimedium include one or more substances selected from the group consisting of Epimedium, Epimedium sagittatum, Epimedium pubescens and Epimedium koreana. Preferably, the plant of the genus Epimedium is one or a combination of Epimedium sagittatum and Epimedium pubescens. More preferably, the plant of the genus Epimedium is Epimedium sagittatum or Epimedium pubescens.
9. The use according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: applying the mixed solution of ethiocarpin to Marchantia odorata in the plants of the genus Epimedium to prevent and control the Marchantia odorata.
10. The use according to claim 9, characterized in that: The steps include: (1) For the first control, use the mixture of ethoxylated chloranthide to apply pesticides to the Marchantia polyphylla in the Epimedium genus. Spray it on the surface or root of the Marchantia polyphylla until the mixture drips down from the surface of the Marchantia polyphylla. Then, spraying is completed. Spraying and watering of the Epimedium genus plants is prohibited within 1 day after application. (2) within 15-30 days after applying the pesticide according to step (1), observe whether there is a recurrence of Marchantia polyphylla. If there is a recurrence of Marchantia polyphylla, apply the mixture of ethyl allicin or ethyl allicin-compound fertilizer to the Marchantia polyphylla of the genus Epimedium. After applying the pesticide, continue to observe for more than 15 days, preferably 15-30 days. If a recurrence of Marchantia polyphylla is observed, repeat the application of the pesticide according to step (2) once. The recurrence of Marchantia polyphylla refers to the appearance of new Marchantia polyphylla thallus structures on the substrate surface of the seedling tray, which is considered a recurrence. (3) After applying the pesticide according to the above step (1) or (2), observation is continued for 15-30 days. If recurrence of Marchantia milii is observed, the compound fertilizer solution is used to apply pesticide to Marchantia milii in the genus Epimedium to complete the prevention and control of Marchantia milii.
11. The use according to claim 10, characterized in that: In step (1), the method of applying the pesticide is spraying.
12. The use according to claim 10, characterized in that: In step (2), the sign of completing the prevention and control of liverwort is that: after applying the compound fertilizer solution or the mixture of ethionine and compound fertilizer until the number of leaves of the Epimedium plant is greater than or equal to 3, and / or the maximum leaf length is greater than or equal to 1 cm, and / or the maximum leaf width is greater than or equal to 1 cm, preferably, the number of leaves is 3-10, and / or the maximum leaf length is 1-10 cm, and / or the maximum leaf width is 1-10 cm, more preferably, the number of leaves is 3-8, and / or the maximum leaf length is 1-5 cm, and / or the maximum leaf width is 1-5 cm; And / or in step (2), the dosage of the pesticide is: spraying the thallus surface of the Marchantia polyphylla and / or the leaf surface of the Epimedium plant seedlings until water flows out from the bottom of the matrix of the seedling tray, so that the thallus surface of the Marchantia polyphylla and / or the roots and leaf surface of the Epimedium plant seedlings have been sprayed.
13. The use according to any one of claims 9 to 12, characterized in that: During the application period, the substrate temperature of the seedling tray is 19-25°C, and the substrate relative humidity of the seedling tray is 60-65%.