Meridian-warming, blood-activating and pain-relieving decoction for treating dysmenorrhea and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to channel-warming, blood-activating and pain-relieving soup for treating dysmenorrhea and a preparation method thereof. The decoction for warming channels, promoting blood circulation and relieving pain is prepared from rhizoma corydalis, rhizoma cyperi, angelica sinensis, ligusticum wallichii, radix paeoniae rubra, radix paeoniae alba, fennel, pollen typhae, rhizoma zingiberis, pericarpium citri reticulatae, cinnamon, radix linderae, frankincense, myrrh, salvia miltiorrhiza and amethyst. The meridian-warming, blood-activating and pain-relieving decoction disclosed by the invention is proper in compatibility, the medicines supplement each other in nature and flavor to meridians, and the medicines are combined to jointly achieve the effects of activating blood and removing stasis and eliminating stagnation and relieving pain, so that the clinical curative effect is improved, the dysmenorrhea symptom of a patient is relieved, the pain-relieving effect is remarkable, and a new way for treating the cold coagulation and blood stasis type dysmenorrhea is opened up.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a meridian-warming, blood-activating and analgesic soup for treating dysmenorrhea and a preparation method thereof. Background Art

[0002] According to the definition of the National Standard of TCM Clinical Diagnosis and Treatment Terminology (Symptoms), blood stasis syndrome refers to internal obstruction of blood stasis and poor blood circulation, with local purple lumps, pain that refuses to be pressed, or abdominal lumps, persistent stabbing pain that refuses to be pressed, or dark purple bleeding in lumps, purple or spotted tongue, and stringy and astringent pulse as common symptoms in TCM. The Yellow Emperor's Classic of Internal Medicine believes that the main etiology and pathogenesis of blood stasis syndrome is that diet, exogenous pathogens, emotions, trauma, and other diseases invade the body, leading to abnormal circulation of qi and blood and stagnation in the pulse. Blood stasis syndrome is relatively common in clinical practice, involving multiple disease systems such as cardiovascular and cerebrovascular, digestive, and gynecological diseases, with a long course of disease and a high prevalence.

[0003] Primary dysmenorrhea is a common gynecological disease. Although there are no pathological changes in the reproductive organs, it has a high incidence and frequent pain, which seriously affects women's study, work and life. Primary dysmenorrhea is mainly divided into six types: cold stagnation and blood stasis, qi stagnation and blood stasis, liver and kidney deficiency, yang deficiency and internal cold, qi and blood deficiency, and damp-heat stasis. Among them, cold stagnation and blood stasis is the most common.

[0004] Dysmenorrhea caused by cold stagnation and blood stasis is related to the "cold" in traditional Chinese medicine. Warming the meridians and dispersing cold, removing blood stasis and relieving pain are important therapies for treating dysmenorrhea caused by cold stagnation and blood stasis in traditional Chinese medicine. Summary of the invention

[0005] The purpose of the present invention is to provide a Wenjing Huoxue Zhitong decoction for treating dysmenorrhea and its preparation method and application according to the deficiencies of the prior art. The Wenjing Huoxue Zhitong decoction of the present invention improves the blood stasis state, has a significant analgesic effect, and has a positive therapeutic effect on dysmenorrhea caused by cold coagulation and blood stasis syndrome.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The first object of the present invention is to provide a meridian-warming, blood-activating and analgesic soup for treating dysmenorrhea.

[0008] The second object of the present invention is to provide a method for preparing the above-mentioned Wenjing Huoxue Zhitong Decoction for treating dysmenorrhea.

[0009] The third object of the present invention is to provide the use of the above-mentioned Wenjing Huoxue Zhitong Decoction for treating dysmenorrhea.

[0010] To achieve the first purpose, the technical solution adopted by the present invention is:

[0011] The invention provides a meridian-warming, blood-activating and analgesic soup for treating dysmenorrhea. The meridian-warming, blood-activating and analgesic soup is prepared from corydalis yanhusuo, cyperus rotundus, angelica sinensis, chuanxiong, red peony root, white peony root, fennel, pollen cattail, dried ginger, tangerine peel, cinnamon bark, lindera strychnifolia, frankincense, myrrh, salvia miltiorrhiza and amethyst.

[0012] Furthermore, the meridian-warming, blood-activating and analgesic soup for treating dysmenorrhea is prepared from the following raw materials in parts by weight: 15-20 parts of Corydalis yanhusuo, 10-15 parts of Cyperus rotundus, 7-11 parts of Angelica sinensis, 7-11 parts of Ligusticum chuanxiong, 7-11 parts of Red Peony Root, 10-15 parts of White Peony Root, 4-8 parts of cumin, 7-11 parts of Pollen Typhae, 4-8 parts of dried ginger, 7-11 parts of dried tangerine peel, 4-8 parts of cinnamon, 7-11 parts of Linderae strychnos, 4-8 parts of frankincense, 4-8 parts of myrrh, 7-11 parts of Salvia miltiorrhiza and 10-15 parts of purple quartz.

[0013] Furthermore, the meridian-warming, blood-activating and analgesic soup for treating dysmenorrhea is prepared from the following raw materials in parts by weight: 18 parts of Corydalis yanhusuo, 12 parts of Cyperus rotundus, 9 parts of Angelica sinensis, 9 parts of Ligusticum chuanxiong, 9 parts of Red Peony Root, 12 parts of White Peony Root, 6 parts of cumin, 9 parts of Pollen Typhae, 6 parts of dried ginger, 9 parts of dried tangerine peel, 6 parts of cinnamon bark, 9 parts of Linderae strychnos, 6 parts of frankincense, 6 parts of myrrh, 9 parts of Salvia miltiorrhiza and 12 parts of purple quartz.

[0014] To achieve the second purpose, the technical solution adopted by the present invention is:

[0015] The preparation method of the warming meridian, activating blood circulation and relieving pain decoction for treating dysmenorrhea of ​​the present invention is as follows: after mixing various medicinal materials, adding water for the first time to decoct for 1.5-2.0 hours, filtering, collecting the first filtrate and filter residue; adding water for the second time to decoct the filter residue for 1.0-1.5 hours, filtering, collecting the second filtrate, and mixing the first filtrate and the second filtrate.

[0016] Furthermore, the amount of water added for the first time is 8-10 times the weight of the mixture; and the amount of water added for the second time is 6-8 times the weight of the mixture.

[0017] Preferably, the Chinese medicine composition further comprises pharmaceutically acceptable excipients, which may include but are not limited to solvents, solubilizers, cosolvents, colorants, disintegrants, fillers, lubricants, wetting agents, stabilizers, flavoring agents, preservatives, suspending agents, coating materials, anti-adhesive agents, integrities, plasticizers, etc.

[0018] The Chinese medicinal materials in the above-mentioned weight portions are taken to prepare the Chinese medicinal raw materials of the present invention, and conventional adjuvants are added according to conventional pharmacy methods to prepare various clinically acceptable dosage forms, which include but are not limited to tablets, capsules, oral liquids, lozenges, granules, granules, pills, drops, powders, pastes, pills, suspensions, powders, solutions, injections, suppositories, ointments, plasters, creams, sprays, and patches. The tablets can be ordinary tablets, sugar-coated tablets, and film tablets, and the capsules can be hard capsules or soft capsules.

[0019] Furthermore, the dosage form of the Wenjing Huoxue Zhitong Decoction for treating dysmenorrhea is decoction, granules, tablets, capsules, mixtures, syrups, pills, gels, powders or pastes.

[0020] To achieve the third purpose, the technical solution adopted by the present invention is:

[0021] The present invention provides use of the Wenjing Huoxue Zhitong decoction for treating dysmenorrhea in preparing a medicine for preventing or treating primary dysmenorrhea due to cold coagulation and blood stasis syndrome.

[0022] Corydalis yanhusuo: pungent and bitter in taste, warm in nature, enters the liver and spleen meridians, promotes blood circulation, promotes qi circulation, and relieves pain; used for chest, flank, abdominal pain, chest obstruction and heart pain, amenorrhea, dysmenorrhea, postpartum blood stasis, and swelling and pain due to falls.

[0023] Cyperus rotundus: spicy, slightly bitter, sweet, neutral in nature, enters the lung, liver, spleen, and stomach meridians, soothes the liver and relieves depression, regulates qi and relieves fullness, regulates menstruation and relieves pain; used for liver depression and qi stagnation, chest and flank distension and pain, hernia pain, breast distension and pain, spleen and stomach qi stagnation, abdominal distension and pain, irregular menstruation, amenorrhea and dysmenorrhea.

[0024] Angelica: spicy and bitter in taste, warm in nature, enters the heart, liver, and spleen meridians, replenishes blood and promotes blood circulation, regulates menstruation and relieves pain, moisturizes and lubricates the intestines; used for irregular menstruation, amenorrhea, dysmenorrhea, accumulation of masses, metrorrhagia, abdominal pain due to deficiency and cold, paralysis, numbness of the skin, dry intestines and difficulty in defecation, heaviness after dysentery, carbuncles, ulcers, and injuries from falls.

[0025] Ligusticum chuanxiong: pungent in taste, warm in nature, enters the liver and gallbladder meridians, promotes qi and relieves depression, eliminates wind, dryness and dampness, promotes blood circulation and relieves pain, treats wind-cold headache and dizziness, flank pain and abdominal pain, cold arthralgia and muscle spasm, amenorrhea, dystocia, postpartum blood stasis and pain, carbuncle and ulcer; used for irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to masses, stabbing pain in the chest and flanks, swelling and pain due to falls, headache, and rheumatic pain.

[0026] Red Peony Root: bitter in taste, slightly cold in nature, enters the Liver Channel, clears away heat and cools blood, disperses blood stasis and relieves pain; used for fever-induced rashes, vomiting blood and epistaxis, red and swollen eyes, liver depression and flank pain, amenorrhea and dysmenorrhea, abdominal pain due to masses, injuries from falls, carbuncles and ulcers.

[0027] White Peony Root: bitter and sour in taste, slightly cold in nature, enters the Liver and Spleen meridians, nourishes blood and regulates menstruation, astringes Yin and stops sweating, softens the liver and relieves pain, and suppresses liver yang; used for blood deficiency and sallow complexion, irregular menstruation, spontaneous sweating, night sweats, flank pain, abdominal pain, cramps in the limbs, headache and dizziness.

[0028] Fennel: spicy in taste, warm in nature, enters the kidney, bladder, and stomach meridians, stimulates appetite, regulates qi and dispels cold, and helps the yang channel; it can treat coldness in the middle burner, loss of appetite, nausea and vomiting, and cold pain in the abdomen; it can also treat hernia pain and testicular swelling and pain; it can also treat spleen and stomach qi stagnation, and abdominal distension and pain.

[0029] Puhuang: tastes bitter and pungent, is warm in nature, enters the spleen and liver meridians, promotes qi and removes blood stasis, regulates menstruation and relieves pain; it can treat chest and abdominal distension and pain, shoulder and arm pain, irregular menstruation, amenorrhea, and injuries from falls.

[0030] Dried ginger: spicy in taste, hot in nature, enters the spleen, stomach, kidney, heart, and lung meridians, and has the effects of warming the middle and dispersing cold, restoring yang and unblocking meridians, warming the lungs and transforming fluid; it is used for symptoms such as cold pain in the abdomen, vomiting and diarrhea, cold limbs and weak pulse, cold fluid and wheezing and coughing.

[0031] Tangerine peel: pungent, bitter, warm in nature, enters the spleen meridian, stomach meridian, and lung meridian, regulates qi and strengthens the spleen, regulates the middle, dries up dampness, and resolves phlegm; it is used to treat abdominal distension or pain, indigestion caused by spleen and stomach qi stagnation, chest tightness, abdominal distension, poor appetite, and loose stools caused by dampness and turbidity blocking the middle. It is used to treat cough and asthma caused by phlegm and dampness blocking the lungs, and is used for chest and abdominal distension, poor appetite, vomiting, diarrhea, and cough with a lot of phlegm.

[0032] Cinnamon: spicy in taste, warm in nature, enters the kidney, spleen, heart and liver meridians, warms and nourishes the body and disperses the qi, has thick and pure yang energy, slowly replenishes kidney yang and replenishes fire to assist yang or guides fire back to the origin, relieves cold and chronic cold and relieves pain, warms and unblocks the meridians and activates blood circulation and disperses blood stasis.

[0033] Lindera strychnifolia: spicy in taste, warm in nature, enters the spleen, stomach, liver, kidney, and bladder meridians, soothes qi, relieves depression, dispels cold, and relieves pain. It is mainly used to treat abdominal distension, cough, asthma, and diarrhea; it is used to treat frequent urination, enuresis, prostatitis, and female dysmenorrhea and postpartum abdominal pain.

[0034] Frankincense: It tastes pungent and bitter, is warm in nature, and enters the heart, liver, and spleen meridians. It regulates qi and promotes blood circulation, relieves pain, and expel toxins. It treats qi and blood stagnation, heart and abdominal pain, carbuncles, swelling and toxins, bruises, dysmenorrhea, and postpartum blood stasis and pain.

[0035] Myrrh: It tastes pungent and bitter, is neutral in nature, and enters the heart, liver, and spleen meridians. It can disperse blood stasis, relieve pain, reduce swelling and promote tissue regeneration. It is used to treat chest pain, heart pain, epigastric pain, dysmenorrhea, amenorrhea, postpartum blood stasis, abdominal pain due to masses, rheumatic pain, traumatic injuries, carbuncles, ulcers and other diseases.

[0036] Salvia miltiorrhiza: bitter in taste, slightly cold in nature, enters the heart and liver meridians, promotes blood circulation and removes blood stasis, cools blood and eliminates carbuncles, clears the heart and relieves restlessness; it is used for symptoms such as irregular menstruation, blood stasis and amenorrhea, abdominal pain due to postpartum blood stasis, heart and abdominal pain, accumulation of lumps and masses, and limb pain.

[0037] Amethyst: sweet in taste, warm in nature, enters the heart, lung, and kidney meridians, calms the mind and soothes the nerves, warms the lungs, and warms the uterus; used for insomnia, palpitations, cough and asthma due to lung deficiency, and infertility due to uterine cold.

[0038] The above-mentioned prescription is properly combined, the nature, flavor and meridians of the drugs complement each other, and the drugs are used together to synergistically enhance their effects, thereby achieving the effects of promoting blood circulation, removing blood stasis, dispersing nodules and relieving pain, improving clinical efficacy, alleviating patients' dysmenorrhea symptoms, and having a significant analgesic effect.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] The present invention adopts injection of epinephrine combined with ice water bath to replicate the cold coagulation and blood stasis model under the joint action of nerve stimulation and cold evil, which well confirms the understanding of traditional Chinese medicine on blood stasis syndrome that "cold causes coagulation, and coagulation causes stasis". Cold coagulation and blood stasis is the pathological basis for the occurrence and development of various diseases. Increased whole blood viscosity and increased plasma viscosity are all related to cold coagulation and blood stasis syndrome. The experimental results show that after the blood stasis model rats were treated with the Wenjing Huoxue Zhitong Decoction of the present invention, the rats' hematocrit values, plasma viscosity, and whole blood viscosity values ​​were significantly reduced, and the blood rheology indicators were significantly improved. The Wenjing Huoxue Zhitong Decoction of the present invention significantly increases the pain threshold of mice induced by hot plate pain, and has a significant analgesic effect.

[0041] The meridian-warming, blood-activating and analgesic decoction of the present invention has a positive therapeutic effect on primary dysmenorrhea due to cold coagulation and blood stasis syndrome.

[0042] In the prescription of the Wenjing Huoxue Zhitong decoction of the present invention, various medicines are used together to play the effects of promoting blood circulation and removing blood stasis, dispersing nodules and relieving pain, improving clinical efficacy, alleviating patients' dysmenorrhea symptoms, and having a significant analgesic effect, thus opening up a new way of medicine for treating cold stagnation and blood stasis type dysmenorrhea. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 Comparison of hematocrit among groups

[0044] Compared with the model control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01.

[0045] Figure 2 Comparison of plasma viscosity among groups

[0046] Compared with the model control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05.

[0047] Figure 3 Comparison of low shear rate among groups

[0048] Compared with the model control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01.

[0049] Figure 4 Comparison of high shear rate among groups

[0050] Compared with the model control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01.

[0051] Figure 5 Comparison of pain threshold among groups

[0052] Compared with the model control group, * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01. DETAILED DESCRIPTION

[0053] The embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only used to illustrate the present invention and should not be construed as limiting the scope of the present invention.

[0054] Example 1

[0055] 18 parts of Corydalis yanhusuo, 12 parts of Cyperus rotundus, 9 parts of Angelica sinensis, 9 parts of Ligusticum chuanxiong, 9 parts of Red Peony Root, 12 parts of White Peony Root, 6 parts of Fennel, 9 parts of Pollen Typhae, 6 parts of Dry Ginger, 9 parts of Tangerine Peel, 6 parts of Cinnamon Bark, 9 parts of Linderae umbellatae, 6 parts of Frankincense, 6 parts of Myrrh, 9 parts of Salvia miltiorrhiza and 12 parts of Amethyst.

[0056] The preparation method is as follows: after mixing the various medicinal materials, add 8 times the weight of water of the mixture for the first time, decoct for 1.5 hours, filter, and collect the first filtrate and residue; add 8 times the weight of water of the mixture to the residue for the second time, decoct for 1.5 hours, filter, and collect the second filtrate, mix the first filtrate and the second filtrate, filter, and take the filtrate to obtain the warming meridians, promoting blood circulation and relieving pain soup.

[0057] Example 2

[0058] 20 parts of Corydalis yanhusuo, 10 parts of Cyperus rotundus, 11 parts of Angelica sinensis, 7 parts of Ligusticum chuanxiong, 11 parts of Red Peony Root, 10 parts of White Peony Root, 4 parts of Cuminum foeniculum, 11 parts of Pollen Typhae, 4 parts of Dry Ginger, 11 parts of Tangerine Peel, 4 parts of Cinnamon Bark, 11 parts of Linderae strychnifolia, 8 parts of Frankincense, 4 parts of Myrrh, 7 parts of Salvia miltiorrhiza and 15 parts of Amethyst.

[0059] The preparation method is as follows: after mixing the various medicinal materials, add 10 times the weight of water of the mixture for the first time, decoct for 2.0 hours, filter, and collect the first filtrate and residue; add 6 times the weight of water of the mixture to the residue for the second time, decoct for 1.0 hour, filter, collect the second filtrate, mix the first filtrate and the second filtrate, filter, and take the filtrate to obtain the warming meridians, promoting blood circulation and relieving pain soup.

[0060] Example 3

[0061] 15 parts of Corydalis yanhusuo, 15 parts of Cyperus rotundus, 7 parts of Angelica sinensis, 11 parts of Ligusticum chuanxiong, 7 parts of Red Peony Root, 15 parts of White Peony Root, 8 parts of Cuminum foeniculum, 7 parts of Pollen Typhae, 8 parts of Dry Ginger, 7 parts of Tangerine Peel, 8 parts of Cinnamon Bark, 7 parts of Linderae umbellatae, 4 parts of Frankincense, 8 parts of Myrrh, 11 parts of Salvia miltiorrhiza and 10 parts of Amethyst.

[0062] The preparation method is as follows: after mixing the various medicinal materials, add 10 times the weight of water of the mixture for the first time, decoct for 2.0 hours, filter, and collect the first filtrate and residue; add 6 times the weight of water of the mixture to the residue for the second time, decoct for 1.0 hour, filter, collect the second filtrate, mix the first filtrate and the second filtrate, filter, and take the filtrate to obtain the warming meridians, promoting blood circulation and relieving pain soup.

[0063] Example 4

[0064] 18 parts of Corydalis yanhusuo, 10 parts of Cyperus rotundus, 11 parts of Angelica sinensis, 10 parts of Ligusticum chuanxiong, 9 parts of Red Peony Root, 12 parts of White Peony Root, 8 parts of Cuminum foeniculum, 9 parts of Pollen Typhae, 6 parts of Dry Ginger, 9 parts of Tangerine Peel, 6 parts of Cinnamon Bark, 8 parts of Linderae umbellatae, 7 parts of Frankincense, 6 parts of Myrrh, 8 parts of Salvia miltiorrhiza and 12 parts of Amethyst.

[0065] The preparation method is as follows: after mixing the various medicinal materials, add 9 times the weight of water of the mixture for the first time, decoct for 1.8 hours, filter, and collect the first filtrate and residue; add 7 times the weight of water of the mixture to the residue for the second time, decoct for 1.3 hours, filter, and collect the second filtrate, mix the first filtrate and the second filtrate, filter, and take the filtrate to obtain the warming meridians, promoting blood circulation and relieving pain soup.

[0066] Example 5

[0067] 15 parts of Corydalis yanhusuo, 18 parts of Cyperus rotundus, 10 parts of Angelica sinensis, 9 parts of Ligusticum chuanxiong, 8 parts of Red Peony Root, 15 parts of White Peony Root, 5 parts of Cuminum foeniculum, 10 parts of Pollen Typhae, 8 parts of Dry Ginger, 9 parts of Tangerine Peel, 6 parts of Cinnamon Bark, 9 parts of Linderae umbellatae, 6 parts of Frankincense, 4 parts of Myrrh, 10 parts of Salvia miltiorrhiza and 15 parts of Amethyst.

[0068] The preparation method is as follows: after mixing the various medicinal materials, add 10 times the weight of water of the mixture for the first time, decoct for 2.0 hours, filter, and collect the first filtrate and residue; add 8 times the weight of water of the mixture to the residue for the second time, decoct for 1.0 hour, filter, collect the second filtrate, mix the first filtrate and the second filtrate, filter, and take the filtrate to obtain the meridian-warming, blood-activating and analgesic soup.

[0069] Example 6

[0070] 20 parts of Corydalis yanhusuo, 10 parts of Cyperus rotundus, 11 parts of Angelica sinensis, 10 parts of Ligusticum chuanxiong, 9 parts of Red Peony Root, 10 parts of White Peony Root, 6 parts of Fennel, 10 parts of Pollen Typhae, 6 parts of Dry Ginger, 10 parts of Tangerine Peel, 6 parts of Cinnamon Bark, 9 parts of Linderae umbellatae, 7 parts of Frankincense, 5 parts of Myrrh, 9 parts of Salvia miltiorrhiza and 12 parts of Amethyst.

[0071] The preparation method is as follows: after mixing the various medicinal materials, add 9 times the weight of water of the mixture for the first time, boil for 2.0 hours, filter, and collect the first filtrate and residue; add 6 times the weight of water of the mixture to the residue for the second time, boil for 1.5 hours, filter, and collect the second filtrate, mix the first filtrate and the second filtrate, filter, and take the filtrate to obtain the warming meridians, promoting blood circulation and relieving pain soup.

[0072] Comparative Example 1

[0073] 15 parts of Cyperus rotundus, 12 parts of Angelica sinensis, 12 parts of Ligusticum chuanxiong, 12 parts of Red Peony Root, 15 parts of White Peony Root, 9 parts of Fennel, 9 parts of Pollen Typhae, 6 parts of Dry Ginger, 9 parts of Tangerine Peel, 6 parts of Cinnamon Bark, 9 parts of Linderae umbellatae, 6 parts of Frankincense, 6 parts of Myrrh, 9 parts of Salvia miltiorrhiza and 12 parts of Amethyst.

[0074] The preparation method is similar to that of Example 1.

[0075] Comparative Example 2

[0076] 20 parts of Corydalis yanhusuo, 11 parts of angelica sinensis, 11 parts of ligusticum chuanxiong, 11 parts of red peony root, 14 parts of white peony root, 8 parts of fennel, 9 parts of pollen cattail, 6 parts of dried ginger, 9 parts of dried orange peel, 6 parts of cinnamon bark, 9 parts of lindera stalk, 6 parts of frankincense, 6 parts of myrrh, 9 parts of salvia miltiorrhiza and 12 parts of purple quartz.

[0077] The preparation method is similar to that of Example 1.

[0078] Comparative Example 3

[0079] 21 parts of Corydalis yanhusuo, 15 parts of Cyperus rotundus, 12 parts of Ligusticum chuanxiong, 9 parts of red peony root, 12 parts of white peony root, 6 parts of fennel, 9 parts of pollen cattail, 6 parts of dried ginger, 9 parts of dried orange peel, 6 parts of cinnamon bark, 9 parts of Linderae umbellatae, 6 parts of frankincense, 6 parts of myrrh, 9 parts of Salvia miltiorrhiza and 12 parts of purple quartz.

[0080] The preparation method is similar to that of Example 1.

[0081] Comparative Example 4

[0082] 21 parts of Corydalis yanhusuo, 15 parts of Cyperus rotundus, 12 parts of Angelica sinensis, 9 parts of Red Peony Root, 12 parts of White Peony Root, 6 parts of Fennel, 9 parts of Pollen Typhae, 6 parts of Dry Ginger, 9 parts of Tangerine Peel, 6 parts of Cinnamon Bark, 9 parts of Linderae umbellatae, 6 parts of Frankincense, 6 parts of Myrrh, 9 parts of Salvia miltiorrhiza and 12 parts of Amethyst.

[0083] The preparation method is similar to that of Example 1.

[0084] Comparative Example 5

[0085] 18 parts of Corydalis yanhusuo, 12 parts of Cyperus rotundus, 9 parts of Angelica sinensis, 9 parts of Ligusticum chuanxiong, 12 parts of fennel, 14 parts of Pollen Typhae, 11 parts of dried ginger, 14 parts of dried tangerine peel, 11 parts of cinnamon bark, 9 parts of Linderae umbellatae, 6 parts of frankincense, 6 parts of myrrh, 9 parts of Salvia miltiorrhiza and 12 parts of purple quartz.

[0086] The preparation method is similar to that of Example 1.

[0087] Comparative Example 6

[0088] 18 parts of Corydalis yanhusuo, 12 parts of costusroot, 9 parts of angelica, 9 parts of ligusticum chuanxiong, 9 parts of red peony root, 12 parts of white peony root, 6 parts of fennel, 9 parts of pollen cattail, 6 parts of dried ginger, 9 parts of dried orange peel, 6 parts of cinnamon bark, 9 parts of lindera stalk, 6 parts of frankincense, 6 parts of myrrh, 9 parts of salvia miltiorrhiza and 12 parts of purple quartz.

[0089] The preparation method is similar to that of Example 1.

[0090] Efficacy Example 1 Experimental study of the Wenjing Huoxue Zhitong decoction of the present invention on blood stasis model

[0091] 1. Establishment of blood stasis model

[0092] 56 SPF grade SD rats, female, weighing 160-180g, 8 were randomly selected as the blank control group, and the remaining rats were divided into 6 groups according to the random number method, 8 rats in each group, namely the model control group, Example 1 group, Example 2 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group.

[0093] The Example 1 group was given 1.6 g / kg of the Example 1 decoction. The Example 2 group was given 1.6 g / kg of the Example 2 decoction. The Comparative Example 1 group was given 1.6 g / kg of the Comparative Example 1 decoction. The Comparative Example 2 group was given 1.6 g / kg of the Comparative Example 2 decoction. The Comparative Example 3 group was given 1.6 g / kg of the Comparative Example 3 decoction. Each group was administered by gavage. Rats in the blank control group and the model control group were gavaged with an equal volume of normal saline at a dose of 10 mL / kg, once a day, for 7 consecutive days. On the 7th day, 0.08 mL / 100 g of epinephrine was subcutaneously injected twice, 4 hours apart, and the rats were immersed in ice water for 5 minutes between the two injections.

[0094] 2. Index detection

[0095] The animals were not fed after treatment, and blood was drawn 1 hour after administration the next morning to measure hematocrit, whole blood viscosity, and plasma viscosity.

[0096] 3. Statistical methods

[0097] SPSS 22.0 software was used for data processing, and the data were expressed as mean ± standard deviation. The data of each group were subjected to normal distribution test and variance homogeneity test, and the inter-group comparison was performed with one-way analysis of variance, with P < 0.05 as the difference being statistically significant.

[0098] 4. Experimental Results

[0099] Compared with the blank control group, the whole blood viscosity and plasma viscosity of the rats in the model control group increased, and the hematocrit value increased, indicating that the acute blood stasis model was successfully established.

[0100] Compared with the model control group, the hematocrit values ​​of rats in Example 1 and Example 2 were reduced. Compared with the comparative example 1-3 groups, there was a significant difference in reducing the hematocrit value in Example 1. Figure 1 .

[0101] Compared with the model control group, the plasma viscosity and whole blood viscosity values ​​of rats in Example 1 and Example 2 were reduced. Compared with the comparative examples 1-3, the Example 1 group had significant differences in reducing the plasma viscosity and whole blood viscosity values. Figure 2-Figure 4 .

[0102] This study used injection of epinephrine combined with an ice water bath to replicate the cold coagulation and blood stasis model under the combined effects of nerve stimulation and cold evil, which well confirmed the understanding of traditional Chinese medicine on blood stasis syndrome that "cold causes coagulation, and coagulation causes stasis". Cold coagulation and blood stasis is the pathological basis for the occurrence and development of many diseases. Increased whole blood viscosity and increased plasma viscosity are all related to cold coagulation and blood stasis syndrome.

[0103] The experimental results show that after the blood stasis model rats were treated with the Wenjing Huoxue Zhitong Decoction of the present invention, the rats' hematocrit value, plasma viscosity and whole blood viscosity value were significantly reduced, and the blood rheology indexes were significantly improved.

[0104] Efficacy Example 2 Experimental study on analgesia of the Wenjing Huoxue Zhitong Decoction of the present invention

[0105] 1. Experimental Methods

[0106] SPF grade Kunming mice, female, weighing 18-20 g, were provided with free access to water and food, and the room temperature was maintained at 20-25°C.

[0107] The temperature of the thermostatic water bath was set to 55°C. When the water temperature rose to (55±0.5)°C, a 500mL beaker was placed in the water bath and preheated for 10 minutes. One mouse was taken each time and placed in the beaker. The time required from the contact of its two feet with the bottom of the beaker to the appearance of licking the hind feet was recorded as the pain threshold of the mouse. Those that did not lick the hind feet within 30 seconds were discarded. After the mouse was marked, the basal pain threshold was measured. The measurement was repeated twice and the average was taken as the basal pain threshold.

[0108] 48 qualified mice were randomly divided into 6 groups, 8 mice in each group, namely model control group, Example 1 group, Example 3 group, Comparative Example 4 group, Comparative Example 5 group, and Comparative Example 6 group.

[0109] The Example 1 group was given 0.9 g / kg of the Example 1 decoction. The Example 3 group was given 0.9 g / kg of the Example 3 decoction. The Comparative Example 4 group was given 0.9 g / kg of the Comparative Example 4 decoction. The Comparative Example 5 group was given 0.9 g / kg of the Comparative Example 5 decoction. The Comparative Example 6 group was given 0.9 g / kg of the Comparative Example 6 decoction. Each group was administered by gavage. The model control group mice were gavaged with an equal volume of normal saline, the dose was 10 mL / kg, once a day, and administered continuously for 3 days.

[0110] 2. Index detection

[0111] The pain threshold was measured 90 minutes after the last medication.

[0112] 3. Statistical methods

[0113] SPSS 22.0 software was used for data processing, and the data were expressed as mean ± standard deviation. The data of each group were subjected to normal distribution test and variance homogeneity test, and the inter-group comparison was performed with one-way analysis of variance, with P < 0.05 as the difference being statistically significant.

[0114] 4. Experimental Results

[0115] Compared with the model control group, Example 1 and Example 3 groups significantly increased the pain threshold of mice induced by hot plate pain. Compared with Comparative Example 4-6 groups, Example 1 group had significant differences in increasing the pain threshold of mice induced by hot plate pain. Figure 5 .

[0116] The experimental results show that the method of warming meridians, promoting blood circulation and relieving pain can significantly increase the pain threshold of mice induced by hot plate pain, and has a significant analgesic effect.

[0117] Clinical case: Observation on the therapeutic effect of Wenjing Huoxue Zhitong Decoction on primary dysmenorrhea due to cold coagulation and blood stasis syndrome

[0118] 1. Materials and Methods

[0119] 1. Clinical data

[0120] A total of 60 patients who were diagnosed with primary dysmenorrhea of ​​cold stagnation and blood stasis type in our outpatient clinic, met the inclusion criteria and could receive systematic observation were selected and randomly divided into a treatment group and a control group, with 30 cases in each group.

[0121] 2. Diagnostic criteria

[0122] Western medicine diagnostic standards: Refer to "Obstetrics and Gynecology", symptoms include lower abdominal pain during or before and after menstruation, accompanied by low back pain, anal pain, and even unbearable pain. The above symptoms are cyclical, and gynecological examination excludes organic genital lesions.

[0123] TCM syndrome diagnosis standard: refer to the TCM clinical syndrome diagnosis and efficacy standard, combined with the TCM clinical diagnosis standard for cold stagnation and blood stasis syndrome differentiation points. Syndrome differentiation standard: main symptoms: cold pain in the lower abdomen before, after or during menstruation, refusing to press, pain relieved by heat; secondary symptoms: scanty menstrual blood, dark menstrual blood with clots; pale complexion; fear of cold, cold limbs, or even coldness; nausea and vomiting, sore waist or anal swelling; tongue: dark tongue, white fur; pulse: slow pulse or tight pulse. The above main symptoms must be present, accompanied by two or more secondary symptoms, and combined with tongue fur and pulse, it can be diagnosed as cold stagnation and blood stasis syndrome.

[0124] 3. Exclusion criteria

[0125] Patients with severe liver and kidney damage and other serious organic diseases; those who cannot take medication on time; those who have participated in other drug research within 3 months.

[0126] 4. Inclusion criteria

[0127] Meet the above diagnostic criteria; age 18-40 years old; have regular menstrual cycles; understand the entire research process and sign the informed consent.

[0128] 5. Treatment Methods

[0129] Control group: Oral administration of ibuprofen sustained-release capsules, 0.4 g each time, twice a day, starting 4 days before the expected menstrual period, for 8 consecutive days.

[0130] Treatment group: Orally take the Wenjing Huoxue Zhitong Decoction prepared in Example 1, 1 dose per day, divided into two doses.

[0131] One course of treatment is 3 months, and you should take one course of treatment.

[0132] 6. Observation of therapeutic effects

[0133] Efficacy criteria

[0134] Cured: abdominal pain and other accompanying symptoms disappeared, and no recurrence was observed after stopping the drug for 3 menstrual cycles; markedly effective: abdominal pain was significantly relieved, accompanying symptoms were relieved or disappeared, and life and work were not affected, and the pain score was improved by more than 1 / 2; effective: abdominal pain was relieved compared with before, and the pain score was 1 / 2-3 / 4 of the original; ineffective: abdominal pain and other symptoms did not change. Total effective rate = (number of cured cases + number of markedly effective cases + number of effective cases) / total number of cases × 100%.

[0135] 7. Statistical methods

[0136] SPSS 22.0 software was used for data processing, and the data were expressed as mean ± standard deviation. The data of each group were subjected to normal distribution test and variance homogeneity test, and the inter-group comparison was performed with one-way analysis of variance, with P < 0.05 as the difference being statistically significant.

[0137] 2. Results

[0138] 1. Comparison of clinical efficacy between the two groups

[0139] The total effective rate of the treatment group was better than that of the control group. The results are shown in Table 1.

[0140] Table 1 Comparison of clinical efficacy between the two groups

[0141]

[0142] The meridian-warming, blood-activating and analgesic decoction of the present invention has a positive therapeutic effect on primary dysmenorrhea due to cold coagulation and blood stasis syndrome.

[0143] The present invention has been described in detail above by general description and specific embodiments, but it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, these modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the scope of protection claimed by the present invention.

Claims

1. A meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea, characterized in that: The meridian-warming, blood-activating and analgesic soup for treating dysmenorrhea is prepared from the following raw materials in parts by weight: 15-20 parts of Corydalis yanhusuo, 10-15 parts of Cyperus rotundus, 7-11 parts of Angelica sinensis, 7-11 parts of Ligusticum chuanxiong, 7-11 parts of Red Peony Root, 10-15 parts of White Peony Root, 4-8 parts of Fennel, 7-11 parts of Pollen Typhae, 4-8 parts of Dry Ginger, 7-11 parts of Tangerine Peel, 4-8 parts of Cinnamon Bark, 7-11 parts of Linderae umbellatae, 4-8 parts of Olibanum, 4-8 parts of Myrrh, 7-11 parts of Salvia miltiorrhiza and 10-15 parts of Amethyst.

2. The meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea according to claim 1, characterized in that: The meridian-warming, blood-activating and analgesic soup for treating dysmenorrhea is prepared from the following raw materials in parts by weight: 18 parts of Corydalis yanhusuo, 12 parts of Cyperus rotundus, 9 parts of Angelica sinensis, 9 parts of Ligusticum chuanxiong, 9 parts of Red Peony Root, 12 parts of White Peony Root, 6 parts of Fennel, 9 parts of Pollen Typhae, 6 parts of Dry Ginger, 9 parts of Tangerine Peel, 6 parts of Cinnamon Bark, 9 parts of Linderae umbellatae, 6 parts of Frankincense, 6 parts of Myrrh, 9 parts of Salvia miltiorrhiza and 12 parts of Amethyst.

3. The meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea according to any one of claims 1 or 2, characterized in that: The meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea further comprises pharmaceutically acceptable adjuvants.

4. The meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea according to claim 3, characterized in that: The Wenjing Huoxue Zhitong decoction for treating dysmenorrhea is a clinically acceptable dosage form.

5. The meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea according to claim 4, characterized in that: The dosage form of the Wenjing Huoxue Zhitong decoction for treating dysmenorrhea is decoction, granules, tablets, capsules, mixtures, syrups, pills, gels, powders or pastes.

6. The meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea according to any one of claims 1 or 2, characterized in that: The preparation method of the Wenjing Huoxue Zhitong Decoction for treating dysmenorrhea is as follows: after mixing various medicinal materials, adding water to boil for the first time for 1.5-2.0 hours, filtering, collecting the first filtrate and filter residue; adding water to boil the filter residue for the second time for 1.0-1.5 hours, filtering, collecting the second filtrate, and mixing the first filtrate and the second filtrate.

7. The meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea according to any one of claims 1 or 2, characterized in that: The preparation method of the Wenjing Huoxue Zhitong Decoction for treating dysmenorrhea is as follows: the amount of water added for the first time is 8-10 times the weight of the mixture; the amount of water added for the second time is 6-8 times the weight of the mixture.

8. Use of the Wenjing Huoxue Zhitong Decoction for treating dysmenorrhea according to any one of claims 1 or 2 in the preparation of a drug for preventing or treating dysmenorrhea.

9. The meridian-warming, blood-activating and analgesic decoction for treating dysmenorrhea according to claim 8, characterized in that: The dysmenorrhea is primary dysmenorrhea due to cold stagnation and blood stasis syndrome.

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