Wubao decoction traditional Chinese medicine composition and application thereof in treating diabetes mellitus

Abstract

The invention provides a Wubao decoction traditional Chinese medicine composition, a preparation method thereof and application of the Wubao decoction traditional Chinese medicine composition in treatment of type 2 diabetes mellitus. The Wubao decoction traditional Chinese medicine composition comprises heat-clearing decoction, Tonghong decoction, heat-clearing decoction, health-preserving decoction and probiotic tea. The heat-clearing soup comprises wrinkled gianthyssop herb, honeysuckle flower, fresh reed rhizome, licorice root, dried ginger, perilla frutescens, apricot kernel, balloonflower root, dahurian angelica root, shaddock peel, jujube and Poria cocos; the dredging soup comprises raspberry, cinnamon, radix angelicae, chicory, liquorice, rhizoma polygonati, hovenia acerba and pomelo peel; the heat-clearing soup comprises honeysuckle, lophatherum gracile, platycodon grandiflorum, liquorice, kudzuvine root, shaddock peel, fresh rhizoma phragmitis, chrysanthemum, folium mori, cape jasmine and semen coicis; the health-preserving soup is prepared from rhizoma polygonati, cortex cinnamomi, poria cocos, liquorice root, semen euryales, longan, fructus lycii, mulberry fruit, fructus rubi, flos caryophylli and shaddock peel; the probiotic tea comprises longan, lotus seeds, white hyacinth beans, poria cocos, liquorice, pericarpium citri reticulatae, perilla frutescens, Chinese yam, shaddock peel and fructus amomi.

Description

Field of the invention:

[0001] The present application relates to the field of traditional Chinese medicine and the field of diabetes treatment. Specifically, the present application provides a Wubao Decoction traditional Chinese medicine composition and its application in the treatment of diabetes. Background technology:

[0002] Type 2 diabetes is a common chronic metabolic disease that requires long-term monitoring and medication, and can cause a series of complications such as diabetic nephropathy, diabetic cardiovascular disease, diabetic neuroinflammation, and diabetic retinopathy. Type 2 diabetes is a heavy burden on both patients and the medical insurance system.

[0003] For chronic diseases such as type 2 diabetes, in addition to controlling diet and taking insulin on time, taking advantage of the conditioning advantages of traditional Chinese medicine is also an effective means to improve the efficacy. The Compendium of Materia Medica believes that grapefruit peel has the effects of digestion, phlegm, and dispersing anger, and can be used to treat a variety of metabolic diseases including diabetes / diabetes. Modern medical research has also found that ingredients such as naringenin in grapefruit peel have the effect of increasing insulin sensitivity. Summary of the invention:

[0004] On the one hand, the present application provides a Wubao Decoction Chinese medicine composition, which includes Qingwen Decoction, Tonghe Decoction, Qingjie Decoction, Yangsheng Decoction and Yisheng Tea;

[0005] The Qingwen Decoction includes Patchouli, Honeysuckle, Fresh Reed Root, Licorice, Dry Ginger, Perilla, Apricot, Platycodon, Angelica Dahurica, Citrus Grapefruit Peel, Jujube, and Poria;

[0006] The Tonghe decoction includes raspberry, cinnamon bark, angelica dahurica, chicory, liquorice, polygonatum, tangerine peel, and grapefruit peel;

[0007] The Qingjie decoction includes honeysuckle, bamboo leaves, platycodon, liquorice, kudzu root, grapefruit peel, fresh reed root, chrysanthemum, mulberry leaf, gardenia, and coix seed;

[0008] The health-preserving soup includes polygonatum, cinnamon bark, poria, licorice, euryale, longan, wolfberry, mulberry, raspberry, clove, and grapefruit peel;

[0009] The beneficial tea comprises longan, lotus seed, white hyacinth bean, tuckahoe, liquorice, tangerine peel, perilla, yam, grapefruit peel and spring amomum.

[0010] Further, the Qingwen Decoction comprises 3-7 parts by weight of Patchouli, 3-5 parts by weight of Honeysuckle, 4-6 parts by weight of fresh Phragmites australis, 1-3 parts by weight of Licorice, 1-2 parts by weight of Dry Ginger, 3-4 parts by weight of Perilla, 3-4 parts by weight of Apricot, 3-4 parts by weight of Platycodon, 3-5 parts by weight of Angelica dahurica, 5-8 parts by weight of Citrus grandiflorum, 4-7 parts by weight of Jujube, and 2-5 parts by weight of Poria.

[0011] The Tonghe decoction comprises 4-7 parts by weight of raspberry, 2-4 parts by weight of cinnamon bark, 2-4 parts by weight of angelica dahurica, 4-7 parts by weight of chrysanthemum, 1-2 parts by weight of liquorice, 3-6 parts by weight of polygonatum, 3-4 parts by weight of citrus aurantium, and 3-5 parts by weight of grapefruit peel;

[0012] The Qingjie decoction comprises 4-6 parts by weight of honeysuckle, 4-6 parts by weight of lonicera japonica, 2-3 parts by weight of platycodon, 1-2 parts by weight of liquorice, 2-3 parts by weight of kudzu root, 1-4 parts by weight of grapefruit peel, 2-4 parts by weight of fresh reed root, 2-4 parts by weight of chrysanthemum, 3-6 parts by weight of mulberry leaf, 1-3 parts by weight of gardenia, and 2-4 parts by weight of coix seed;

[0013] Further, the Qingwen Decoction is prepared from 5 parts by weight of Patchouli, 4 parts by weight of Honeysuckle, 5 parts by weight of fresh Phragmites australis, 1 part by weight of Licorice, 1 part by weight of Dry Ginger, 3 parts by weight of Perilla, 3 parts by weight of Apricot, 3 parts by weight of Platycodon, 4 parts by weight of Angelica dahurica, 7 parts by weight of Citrus grandiflorum, 5 parts by weight of Jujube, and 3 parts by weight of Poria;

[0014] The Tonghe decoction is prepared from 5 parts by weight of raspberry, 3 parts by weight of cinnamon bark, 3 parts by weight of angelica dahurica, 5 parts by weight of chicory, 2 parts by weight of liquorice, 4 parts by weight of polygonatum, 3 parts by weight of citrus aurantium, and 3 parts by weight of grapefruit peel;

[0015] The Qingjie decoction is prepared from 5 parts by weight of honeysuckle, 5 parts by weight of lophatherum gracile, 3 parts by weight of platycodon grandiflorum, 1 part by weight of liquorice, 2 parts by weight of kudzu root, 2 parts by weight of grapefruit peel, 3 parts by weight of fresh reed root, 3 parts by weight of chrysanthemum, 5 parts by weight of mulberry leaf, 2 parts by weight of gardenia jasminoides, and 3 parts by weight of coix seed;

[0016] Further, the health-preserving soup is prepared from 3 parts by weight of polygonatum, 2 parts by weight of cinnamon bark, 4 parts by weight of poria, 1 part by weight of licorice, 2 parts by weight of euryale, 5 parts by weight of longan, 3 parts by weight of wolfberry, 3 parts by weight of mulberry, 1 part by weight of raspberry, 1 part by weight of clove, and 2 parts by weight of grapefruit peel;

[0017] The probiotic tea is prepared from 3 parts by weight of longan, 1 part by weight of lotus seeds, 1 part by weight of white hyacinth bean, 3 parts by weight of Poria cocos, 1 part by weight of liquorice, 3 parts by weight of dried tangerine peel, 1 part by weight of perilla, 3 parts by weight of yam, 2 parts by weight of grapefruit peel and 1 part by weight of Amomum villosum.

[0018] Furthermore, the composition is in the form of a tea substitute; preferably packaged in a tea bag.

[0019] On the other hand, the present application provides a method for preparing the above-mentioned Wubao Decoction Chinese medicine composition, which comprises (1) weighing and crushing the raw materials; (2) mixing the raw materials of Qingwen Decoction, Tonghe Decoction, Qingjie Decoction, Yangsheng Decoction and Yisheng Tea respectively; and (3) packaging.

[0020] Furthermore, the preparation method also includes a sterilization step.

[0021] The composition of the present application can be in the form of a brewed / boiled liquid or a solid form of powder / fragments. The Chinese medicine composition of the present application can be processed by crushing, mixing, and sterilization methods known in the art. For the purpose of adjusting the taste and preservation, pharmaceutically acceptable masking agents, flavor correctors, sweeteners, acidulants, preservatives, antioxidants, and other ingredients can be used.

[0022] On the other hand, the present application provides the use of the above-mentioned Wubao Decoction Chinese medicine composition in the preparation of a drug for treating diabetes.

[0023] Furthermore, the diabetes is type 2 diabetes.

[0024] When describing the proportions of the Chinese medicine composition in this application, unless otherwise specified, all are parts by weight or weight ratios.

[0025] When administered, according to the patient's condition (e.g., physical conditions such as excessive fire, yin deficiency, etc.), the Qingwen Decoction, Tonghe Decoction, Qingjie Decoction, Yangsheng Decoction and Yisheng Tea in the pharmaceutical composition of the present application can be consumed continuously for 1-3, 1-3, 1-3, 1-3, 3-5 days in sequence as a course of treatment. Description of the drawings:

[0026] Figure 1 The insulin resistance index of each group at different times is shown;

[0027] Figure 2 The glucagon test values ​​of each group at different times are shown. Specific implementation method:

[0028] Example 1 Preparation of Chinese medicine composition

[0029] The Wubao Decoction Chinese medicine composition was prepared according to the following ratio:

[0030] Qingwen Decoction: 5 parts by weight of Patchouli, 4 parts by weight of Honeysuckle, 5 parts by weight of fresh Phragmites australis, 1 part by weight of Licorice, 1 part by weight of Dried Ginger, 3 parts by weight of Perilla, 3 parts by weight of Apricot, 3 parts by weight of Platycodon, 4 parts by weight of Angelica dahurica, 7 parts by weight of Citrus grandiflorum, 5 parts by weight of Jujube, and 3 parts by weight of Poria;

[0031] Tonghe Decoction: 5 parts by weight of raspberry, 3 parts by weight of cinnamon bark, 3 parts by weight of angelica dahurica, 5 parts by weight of chicory, 2 parts by weight of liquorice, 4 parts by weight of polygonatum, 3 parts by weight of citrus aurantium, and 3 parts by weight of grapefruit peel;

[0032] Qingjie Decoction: 5 parts by weight of honeysuckle, 5 parts by weight of loblolly leaves, 3 parts by weight of platycodon, 1 part by weight of liquorice, 2 parts by weight of kudzu root, 2 parts by weight of grapefruit peel, 3 parts by weight of fresh reed root, 3 parts by weight of chrysanthemum, 5 parts by weight of mulberry leaf, 2 parts by weight of gardenia, 3 parts by weight of coix seed;

[0033] Health-preserving soup: 3 parts by weight of polygonatum, 2 parts by weight of cinnamon bark, 4 parts by weight of poria, 1 part by weight of liquorice, 2 parts by weight of euryale, 5 parts by weight of longan, 3 parts by weight of wolfberry, 3 parts by weight of mulberry, 1 part by weight of raspberry, 1 part by weight of clove, and 2 parts by weight of grapefruit peel;

[0034] Probiotic tea: 3 parts by weight of longan, 1 part by weight of lotus seeds, 1 part by weight of white hyacinth bean, 3 parts by weight of Poria cocos, 1 part by weight of licorice, 3 parts by weight of tangerine peel, 1 part by weight of perilla, 3 parts by weight of yam, 2 parts by weight of grapefruit peel, and 1 part by weight of Amomum villosum.

[0035] Add 50 times the weight of water to the ingredients of each soup, boil for 15 minutes and filter out the soup; the soup can be stored at 4°C for later use.

[0036] Example 2 Main reagents and model

[0037] Main detection reagents:

[0038] Blood glucose meter and test strips: Roche Rat serum insulin detection kit: ELISA, Aimijie;

[0039] Rat serum glucagon kit: ELISA, Shanghai Xitang;

[0040] Glycated hemoglobin A1 / c test kit: Zhongke Quality Inspection;

[0041] Insulin resistance index = fasting blood glucose × serum insulin / 22;

[0042] Model Animal Overview:

[0043] Male SD rats for the experiment were purchased from Liaoning Changsheng Biotechnology Co., Ltd., 8-10 weeks old, weighing 200±15g. After the experimental animals arrived, they were adaptively fed with the following basic complete nutrition feed for 10 days in the experimental unit before modeling.

[0044] Model animal breeding conditions:

[0045] The temperature in the breeding box was set at 22°C, humidity at 40%, light at 150Lx, and a 12 / 12 hour day and night rhythm; the basic complete nutrition feed and high-sugar and high-fat feed (indicated that sucrose, lard, and cholesterol were added to the basic complete nutrition feed) were purchased from Jiangsu Collaborative Biology.

[0046] Modeling process of type 2 diabetes rat model:

[0047] A 10 mg / mL streptozotocin (STZ) solution was prepared using citric acid-sodium citrate buffer (0.1 M, pH 4.5).

[0048] The mice were fed with a high-sugar and high-fat diet for 4 weeks. On the first day of the fifth week, a single intraperitoneal injection of the freshly prepared STZ solution was given at a dose of 6 mL / mouse.

[0049] On the 4th day after injection, blood was collected from the tail vein to test fasting blood glucose. A successful modeling was considered if the fasting blood glucose was greater than 11 mmol / L.

[0050] Example 3 Experimental process

[0051] In order to reduce experimental factors, the combined effects of Qingwen Decoction, Tonghe Decoction, and Qingjie Decoction, which are the main therapeutic effects on diabetes, were investigated in Wubao Decoction, while the tonic health-preserving soup and probiotic tea were not used for the time being. The dosage of various soups for rats was about 2 mL / day according to the body weight ratio, and was given by gavage at one time. Groups 1-7 were set up according to different dosing methods, and model groups and normal groups were also set up; the number of rats in each group was 15; all rats were given basic complete nutritional feed during the experiment.

[0052] Table 1 Grouping and dosing regimen

[0053]

[0054] The start time of drug administration was counted as day 1, and the testing time was selected on day 11, day 33, day 44 (5 days after the last medication), and then on day 60 (3 weeks after the last medication).

[0055] Example 4 Experimental results

[0056] During the 60-day experiment, one rat died in the model group and one rat in group 6. The experimental results of the remaining rats were statistically analyzed as follows:

[0057] Blood sugar status:

[0058] Table 2 Fasting blood glucose values

[0059]

[0060]

[0061] As shown in Table 2, the fasting blood glucose level of the normal group was basically at the level of 4-5; while the model group gradually increased from about 16 at the beginning of the experiment to about 27-28 after 33 days. Among the 7 experimental groups, groups 1-3 and 5 all produced a relatively obvious hypoglycemic effect, especially group 1, whose blood glucose level remained basically the same as that on the 44th day 3 weeks after drug withdrawal, while the blood glucose levels of groups 2, 3, and 5 rebounded significantly 3 weeks after drug withdrawal.

[0062] Table 3 Glycated hemoglobin values

[0063]

[0064] The glycated hemoglobin values ​​shown in Table 3 further confirmed the results of Table 1. The glycated hemoglobin values ​​of Group 1 at 60 days were significantly lower than those of the other groups, indicating that their blood sugar was generally lower for a period of time after treatment. In addition, Group 5 also had a better effect in this regard.

[0065] Insulin sensitivity:

[0066] like Figure 1 As shown, the insulin resistance index of the normal group was at the level of 2.3-2.7; while the model group gradually increased from about 13 at the beginning of the experiment to about 24-26 after 33 days. Among the 7 experimental groups, groups 1-3, 5, and 7 produced a more obvious effect of reducing the insulin resistance index, especially the combined medication groups 1, 5, and 7 had certain advantages in long-term effects compared with the single medication groups 2 and 3. It shows that Qingwen Decoction combined with other decoctions can effectively improve insulin sensitivity.

[0067] Glucagon status:

[0068] like Figure 2 As shown, the glucagon level of the normal group was around 100, while that of the model group gradually increased from around 118 at the beginning of the experiment to around 130 after 33 days. Among the 7 experimental groups, Group 1 and Group 5 produced a significant effect of inhibiting the increase of glucagon. This shows that Qingwen Decoction combined with Tonghe Decoction has the effect of inhibiting the power of blood sugar increase.

[0069] Observations on the weight changes and appearance of the animals showed that at day 60, the weight of the mice in groups 1, 3, and 5 was 18-20% higher than that of the model group, and their fur quality and behavior were also significantly better than those of the model group.

[0070] It can be expected that Group 1 and Group 5 can achieve better results after taking the tonic health-preserving soup and probiotic tea.

Claims

1. A Wubao Decoction Chinese medicine composition, characterized in that: The Wubao decoction Chinese medicine composition comprises Qingwen decoction, Tonghe decoction, Qingjie decoction, Yangsheng decoction and Yisheng tea; The Qingwen Decoction includes Patchouli, Honeysuckle, Fresh Reed Root, Licorice, Dry Ginger, Perilla, Apricot, Platycodon, Angelica Dahurica, Citrus Grapefruit Peel, Jujube, and Poria; The Tonghe decoction includes raspberry, cinnamon bark, angelica dahurica, chicory, liquorice, polygonatum, tangerine peel, and grapefruit peel; The Qingjie decoction includes honeysuckle, bamboo leaves, platycodon, liquorice, kudzu root, grapefruit peel, fresh reed root, chrysanthemum, mulberry leaf, gardenia, and coix seed; The health-preserving soup includes polygonatum, cinnamon bark, poria, licorice, euryale, longan, wolfberry, mulberry, raspberry, clove, and grapefruit peel; The beneficial tea comprises longan, lotus seed, white hyacinth bean, tuckahoe, liquorice, tangerine peel, perilla, yam, grapefruit peel and spring amomum.

2. The Chinese medicinal composition of Wubao Decoction according to claim 1, wherein the Qingwen Decoction comprises 3-7 parts by weight of Patchouli, 3-5 parts by weight of Honeysuckle, 4-6 parts by weight of fresh Phragmites australis, 1-3 parts by weight of Licorice, 1-2 parts by weight of Dry Ginger, 3-4 parts by weight of Perilla, 3-4 parts by weight of Apricot, 3-4 parts by weight of Platycodon, 3-5 parts by weight of Angelica dahurica, 5-8 parts by weight of Citrus grandiflorum, 4-7 parts by weight of Jujube, and 2-5 parts by weight of Poria; The Tonghe decoction comprises 4-7 parts by weight of raspberry, 2-4 parts by weight of cinnamon bark, 2-4 parts by weight of angelica dahurica, 4-7 parts by weight of chrysanthemum, 1-2 parts by weight of liquorice, 3-6 parts by weight of polygonatum, 3-4 parts by weight of citrus aurantium, and 3-5 parts by weight of grapefruit peel; The Qingjie Decoction comprises 4-6 parts by weight of honeysuckle, 4-6 parts by weight of loblolly leaves, 2-3 parts by weight of platycodon, 1-2 parts by weight of liquorice, 2-3 parts by weight of kudzu root, 1-4 parts by weight of grapefruit peel, 2-4 parts by weight of fresh reed root, 2-4 parts by weight of chrysanthemum, 3-6 parts by weight of mulberry leaf, 1-3 parts by weight of gardenia, and 2-4 parts by weight of coix seed.

3. The Chinese medicine composition of Wubao Decoction according to claim 1, wherein the Qingwen Decoction is prepared by 5 parts by weight of Patchouli, 4 parts by weight of Honeysuckle, 5 parts by weight of fresh Phragmites australis, 1 part by weight of Licorice, 1 part by weight of Dried Ginger, 3 parts by weight of Perilla, 3 parts by weight of Apricot, 3 parts by weight of Platycodon, 4 parts by weight of Angelica dahurica, 7 parts by weight of Citrus grandiflorum, 5 parts by weight of Jujube, and 3 parts by weight of Poria; The Tonghe decoction is prepared from 5 parts by weight of raspberry, 3 parts by weight of cinnamon bark, 3 parts by weight of angelica dahurica, 5 parts by weight of chicory, 2 parts by weight of liquorice, 4 parts by weight of polygonatum, 3 parts by weight of citrus aurantium, and 3 parts by weight of grapefruit peel; The Qingjie Decoction is prepared from 5 parts by weight of honeysuckle, 5 parts by weight of loblolly leaves, 3 parts by weight of platycodon, 1 part by weight of liquorice, 2 parts by weight of kudzu root, 2 parts by weight of grapefruit peel, 3 parts by weight of fresh reed root, 3 parts by weight of chrysanthemum, 5 parts by weight of mulberry leaves, 2 parts by weight of gardenia, and 3 parts by weight of coix seed.

4. The Chinese medicinal composition of Wubao Decoction according to claim 3, wherein the health-preserving soup is prepared from 3 parts by weight of polygonatum, 2 parts by weight of cinnamon bark, 4 parts by weight of tuckahoe, 1 part by weight of liquorice, 2 parts by weight of euryale, 5 parts by weight of longan, 3 parts by weight of wolfberry, 3 parts by weight of mulberry, 1 part by weight of raspberry, 1 part by weight of clove, and 2 parts by weight of grapefruit peel; The probiotic tea is prepared from 3 parts by weight of longan, 1 part by weight of lotus seeds, 1 part by weight of white hyacinth bean, 3 parts by weight of Poria cocos, 1 part by weight of liquorice, 3 parts by weight of dried tangerine peel, 1 part by weight of perilla, 3 parts by weight of yam, 2 parts by weight of grapefruit peel and 1 part by weight of Amomum villosum.

5. The Wubao Decoction Chinese medicine composition according to any one of claims 1 to 4, which is in the form of a substitute tea.

6. The Wubao Decoction Chinese medicine composition according to claim 5, wherein the Qingwen Decoction, Tonghe Decoction, Qingjie Decoction, Yangsheng Decoction and Yisheng Tea in the Wubao Decoction Chinese medicine composition are respectively packed in tea bags.

7. The method for preparing the Wubao Decoction Chinese medicine composition according to any one of claims 1 to 6, characterized in that: The method comprises: (1) Weighing and crushing raw materials; (2) respectively mixing the raw materials of Qingwen Decoction, Tonghe Decoction, Qingjie Decoction, Yangsheng Decoction and Yisheng Tea; (3) Packaging. The preparation method according to claim 7 , further comprising a sterilization step.

9. Use of the Wubao Decoction Chinese medicine composition according to any one of claims 1 to 6 in the preparation of a medicine for treating diabetes.

10. The use according to claim 9, wherein the diabetes is type 2 diabetes.

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