Melanocyte dissolution and separation device and use method

Abstract

The invention discloses a melanocyte dissolution and separation device and a use method. The melanocyte dissolution and separation device comprises a case and a lifting assembly, a central shaft is rotationally connected in the case, a placement groove is formed in the upper end of the central shaft, supporting seats which are uniformly distributed are arranged at the upper end of the outer arc surface of the central shaft, rotating shafts are rotationally connected to the ends, away from the central shaft, of the multiple supporting seats, test tube seats are arranged outside the multiple rotating shafts, and adjustable tube seat assemblies are movably clamped to the bottoms of the test tube seats; according to the melanocyte dissolution and separation device and the use method, the test tube seat is provided with the adjustable base, so that test tubes with different lengths can be conveniently placed, the test tubes can be automatically clamped after being inserted, the angle of the test tube group is adjustable, the use flexibility is good, and the test tube group is convenient to use and high in practicability. Supernate in a plurality of test tubes can be discharged synchronously, and the use convenience is good.

Description

Technical Field

[0001] The present invention relates to the technical field of melanocyte separation, and in particular to a melanocyte dissolution and separation device and a method for using the same. Background Art

[0002] Melanocytes are special cells in the skin that produce melanin and transfer it to the surrounding keratinocytes. Melanin stays in the nuclei of these keratinocytes to protect them from being damaged by light radiation. A melanocyte dissolution and separation device is used to separate the melanocytes. During the operation, the skin tissue containing melanocytes is dissolved and then filtered. The filtered solution is centrifuged using a melanocyte dissolution and separation device, and the supernatant is poured out to obtain the melanocytes. Existing melanocyte dissolution and separation devices generally use a motor to drive the test tube holder to rotate, and the test tube holder with the test tube inserted inside rotates for centrifugal separation. The structure is simple and the cost of use is low, but the depth and angle of the test tube holder placement hole are generally not adjustable, and the fixation effect of the test tube is poor. After centrifugation, the supernatant in the test tube needs to be poured out one by one, and the convenience of use is poor. Summary of the Invention

[0003] The technical problem to be solved by the present invention is to overcome the existing defects and provide a melanocyte dissolution and separation device and a method of use. The test tube holder is provided with an adjustable base to facilitate the placement of test tubes of different lengths. The test tube will be automatically clamped after insertion, and the angle of the test tube group is adjustable. It has good flexibility in use and can discharge the supernatant in multiple test tubes synchronously. It is easy to use and can effectively solve the problems in the background technology.

[0004] To achieve the above-mentioned object, the present invention provides the following technical solutions: a melanocyte dissolution and separation device, comprising a chassis and a lifting assembly;

[0005] Chassis: A central shaft is rotatably connected inside the chassis, a placement slot is provided at the upper end of the central shaft, evenly distributed support seats are provided at the upper end of the outer arc surface of the central shaft, multiple support seats are rotatably connected to the rotating shaft at one end away from the central shaft, test tube seats are provided on the outside of the multiple rotating shafts, and adjustable tube seat assemblies are movably connected to the bottom of the test tube seats, gears are provided in the middle of the outer arc surfaces of the multiple rotating shafts, racks are slidably connected to the inside of the multiple support seats, and the racks and gears inside the same support seat are meshed and connected;

[0006] Lifting assembly: used to control the up and down movement of multiple racks. The lower end of the test tube holder is movably connected to an adjustable base, which facilitates the fixation of centrifugal test tubes of different lengths. After the test tube is inserted, the adjustable tube holder assembly can automatically clamp the test tube, which has a simple structure and is easy to use. It has a good fixing effect on the test tube for melanocyte dissolution and separation, can control multiple test tube holders to rotate synchronously, and is convenient for adjusting the centrifugal angle. At the same time, after centrifugation, it can also control multiple test tubes to discharge the supernatant synchronously, which is convenient to use in melanocyte dissolution and separation.

[0007] Furthermore, the lifting assembly includes a moving seat, a nut, a screw seat, a screw and a support bar. The moving seat is slidably connected to the middle part of the outer arc surface of the central shaft. Evenly distributed support bars are provided around the outer arc surface of the moving seat. The upper ends of multiple support bars are fixedly connected to the vertically corresponding racks. Two screw seats are provided in the middle part of the outer arc surface of the central shaft. A screw is rotatably connected between the two screw seats. A nut is provided on the outer arc surface of the moving seat. The nut is threadably connected to the screw, which facilitates the control of the up and down movement of the rack.

[0008] Furthermore, a counterweight bar is provided on the outer arc surface of the movable seat, and the counterweight bar is arranged in cooperation with the screw rod. The counterweight bar improves the uniformity of weight distribution around the central axis and balances the weight of the screw rod, nut and screw rod seat.

[0009] Furthermore, it also includes a pointer and a dial, the dials are respectively arranged on the upper end of the test tube holder, and the pointers are respectively arranged on the side of the support seat. The pointer and the dial are arranged in coordination to facilitate determination of the angle of the test tube holder.

[0010] Furthermore, it also includes positioning plates, which are respectively arranged at the upper ends of the racks to prevent the racks from separating from the support seat.

[0011] Furthermore, the adjustable tube holder assembly includes a base, a pin shaft, a clamping plate, a gear slot and a spring sheet. The bases are respectively slidably connected to the inside of the test tube holders. The outer arc surfaces of multiple test tube holders are provided with symmetrically distributed gear slots. The upper ends of multiple bases are provided with two pin shafts. The outer arc surfaces of multiple pin shafts are rotatably connected with clamping plates. The upper ends of multiple bases are provided with two spring sheets. The spring sheets and the gear slots are cooperated with the clamping plates to facilitate the support of test tubes of different lengths.

[0012] Furthermore, the adjustable tube holder assembly further includes a rubber strip, which is respectively arranged at one end of the clamping plate extending into the interior of the test tube holder to facilitate clamping of the test tube.

[0013] Furthermore, a control switch is provided on the left side of the upper surface of the chassis, and a motor is provided at the lower end inside the chassis. The output shaft of the motor is fixedly connected to the lower end of the central shaft, the input end of the motor is electrically connected to the output end of the control switch, and the input end of the control switch is electrically connected to an external power supply to facilitate driving the rotation of the central shaft.

[0014] A method for using a melanocyte dissolution and separation device comprises the following steps:

[0015] (S1: Adjust the depth of the test tube holder: Press the lower end of the card plate with two fingers. The card plate rotates around the pin and its upper end rises. The upper end of the card plate moves out of the gear slot while squeezing the spring sheet. Then, pinch the two card plates to drive the base to slide along the gear slot. Release the card plates. The upper end of the card plate is movably engaged with the horizontal groove of the gear slot.

[0016] (S2: Adjust the angle of the test tube holder: rotate the screw, which drives the movable seat to move up and down along the central axis through the nut. The movable seat drives the rack to slide vertically along the support seat through the support bar. The rack drives the test tube holder to rotate through the gear and the rotating shaft. The angle between the test tube holder and the central axis is between - degrees;

[0017] (S3: Place the test tube: insert the test tube containing the melanocyte solution into the test tube holder, so that the bottom of the test tube contacts the base. Under the action of the spring sheet, the clamping plate clamps the test tube through the rubber strip;

[0018] (S4: Centrifugal precipitation: Adjust the control switch, the motor starts to operate, the output shaft of the motor drives the central shaft to rotate, and the central shaft drives the tilted test tube to rotate through the support base, the rotating shaft and the test tube holder, and rotates for three to five minutes;

[0019] (S5: Pour out the supernatant: Rotate the screw to control the rack to move downward, driving the lower end of the test tube holder to rotate upward. The supernatant on the upper layer of the test tube flows out from the port and flows into the supernatant collection tube. After the supernatant is discharged, rotate the screw in the opposite direction to control the rack to move upward, and then remove the test tube.

[0020] Compared with the prior art, the beneficial effects of the present invention are: the melanocyte dissolution and separation device and the method of use have the following advantages:

[0021] 1. The lower end of the test tube holder is movably connected with an adjustable base, which facilitates the fixation of centrifugal test tubes of different lengths. After the test tube is inserted, the adjustable tube holder assembly can automatically clamp the test tube, which has a simple structure and is easy to use. It has a good fixation effect on the test tube for melanocyte dissolution and separation.

[0022] 2. It can control multiple test tube holders to rotate synchronously, which is convenient for adjusting the centrifugal angle. At the same time, after centrifugation, it can also control multiple test tubes to discharge the supernatant synchronously, which is convenient to use in melanocyte dissolution and separation. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the structure of the present invention;

[0024] Figure 2It is a schematic cross-sectional structural diagram of the chassis of the present invention;

[0025] Figure 3 This is an enlarged structural diagram of point A of the present invention;

[0026] Figure 4 This is an enlarged structural diagram of point B of the present invention;

[0027] Figure 5 Schematic diagram of the cross-sectional structure of the test tube holder of the present invention;

[0028] Figure 6 This is an enlarged structural diagram of point C of the present invention.

[0029] In the figure: 1 chassis, 2 center axis, 3 placement slot, 4 test tube holder, 5 adjustable tube holder assembly, 51 base, 52 pin shaft, 53 clamping plate, 54 gear slot, 55 spring sheet, 56 rubber strip, 6 rotating shaft, 7 gear, 8 rack, 9 lifting assembly, 91 moving seat, 92 nut, 93 screw seat, 94 screw, 95 support bar, 10 control switch, 11 motor, 12 counterweight bar, 13 pointer, 14 dial, 15 positioning plate, 16 support seat. DETAILED DESCRIPTION

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] See also Figure 1-6 , this embodiment provides a technical solution: a melanocyte dissolution and separation device, including a chassis 1 and a lifting component 9;

[0032] Chassis 1: It is internally connected to a central shaft 2 for rotation. Chassis 1 provides protection for internal components. A placement slot 3 is provided at the upper end of the central shaft 2. The supernatant collection tube is placed inside the placement slot 3. The upper end of the outer arc surface of the central shaft 2 is provided with evenly distributed support seats 16. The ends of multiple support seats 16 away from the central shaft 2 are all rotatably connected to the rotating shaft 6. There are four support seats 16. The outsides of multiple rotating shafts 6 are all provided with test tube seats 4. The central shaft 2 drives the inclined test tube to rotate through the support seat 16, the rotating shaft 6 and the test tube seat 4, and uses the difference in sedimentation rate of different cell components under the action of centrifugal force to achieve separation, driving the lower end of the test tube seat 4 to rotate upward, and the internal test tube will rotate to a horizontal state, and the supernatant on the upper layer of the test tube will flow out from the port. And flows into the supernatant collection tube, the bottom of the test tube holder 4 is movably connected with an adjustable tube holder assembly 5, the middle part of the outer arc surface of the multiple rotating shafts 6 is provided with a gear 7, the interior of the multiple support seats 16 is slidably connected with a rack 8, the rack 8 and the gear 7 inside the same support seat 16 are meshed and connected, and the rack 8 drives the test tube holder 4 to rotate through the gear 7 and the rotating shaft 6, and also includes a pointer 13 and a dial 14, the dial 14 is respectively arranged on the upper end of the test tube holder 4, the pointer 13 is respectively arranged on the side of the support seat 16, the pointer 13 and the dial 14 are matched with each other, and the test tube holder 4 drives the dial 14 to rotate around the axis of the rotating shaft 6, and then the position of the dial 14 and the pointer 13 can be observed to determine the angle of the test tube holder 4, and then the centrifugal angle of the test tube is determined. The positioning plates 15 are respectively arranged on the upper ends of the racks 8. The positioning plates 15 prevent the racks 8 from moving out of the support base 16. The adjustable tube seat assembly 5 includes a base 51, a pin 52, a card plate 53, a gear slot 54 and a spring piece 55. The bases 51 are respectively slidably connected to the inside of the test tube seat 4. There are four bases 51 and four test tube seats 4. A plurality of test tube seats 4 are provided with symmetrically distributed gear slots 54 on the outer arc surfaces. The upper ends of the plurality of bases 51 are respectively provided with two pins 52. The outer arc surfaces of the plurality of pins 52 are rotatably connected with card plates 53. The upper ends of the plurality of bases 51 are respectively provided with two spring pieces 55. The spring piece 55 and the gear slot 54 are both arranged in conjunction with the card plate 53. The lower end of the card plate 53 can be pressed with two fingers. 3 rotates around the pin shaft 52 and its upper end is tilted, the upper end of the card plate 53 moves out of the gear slot 54 and squeezes the spring piece 55 at the same time, and then the two card plates 53 are pinched to drive the base 51 to slide along the gear slot 54. The gear slot 54 is composed of a vertical slot parallel to the axis of the test tube holder 4 and a plurality of transverse slots perpendicular to the axis. When it moves to the appropriate position, the upper end of the card plate 53 corresponds to the transverse slot of the gear slot 54, and then the card plate 53 is released. The elastically deformed spring piece 55 presses the card plate 53 downward to reset it, and the upper end of the card plate 53 is movably engaged with the transverse slot of the gear slot 54. The adjustable tube holder assembly 5 also includes a rubber strip 56, which is respectively arranged at one end of the card plate 53 extending to the inside of the test tube holder 4. A test tube containing melanocyte solution is inserted into the inside of the test tube holder 4.The bottom of the test tube contacts the base 51. At the same time, under the action of the spring sheet 55, the clamping plate 53 clamps the test tube through the rubber strip 56, thereby achieving the fixation of the test tube. A control switch 10 is provided on the left side of the upper surface of the chassis 1. A motor 11 is provided at the lower end of the interior of the chassis 1. The output shaft of the motor 11 is fixedly connected to the lower end of the central shaft 2. The input end of the motor 11 is electrically connected to the output end of the control switch 10. The input end of the control switch 10 is electrically connected to an external power supply. When the control switch 10 is adjusted, the motor 11 runs, and the output shaft of the motor 11 drives the central shaft 2 to rotate.

[0033] Lifting assembly 9: used to control the up and down movement of multiple racks 8. The lifting assembly 9 includes a moving seat 91, a nut 92, a screw seat 93, a screw 94 and a support bar 95. The moving seat 91 is slidably connected to the middle of the outer arc surface of the central shaft 2. A uniformly distributed support bar 95 is provided around the outer arc surface of the moving seat 91. The upper ends of the multiple support bars 95 are fixedly connected to the vertically corresponding racks 8. Two screw seats 93 are provided in the middle of the outer arc surface of the central shaft 2. A screw 94 is rotatably connected between the two screw seats 93. The screw seat 93 provides a screw 94 with a support. Rotational support, a nut 92 is provided on the outer arc surface of the movable seat 91, and the nut 92 is threadedly connected to the screw rod 94. The screw rod 94 is rotated, and the screw rod 94 drives the movable seat 91 to move up and down along the central axis 2 through the nut 92. The movable seat 91 drives the rack 8 to slide vertically along the support seat 16 through the support bar 95. A counterweight bar 12 is provided on the outer arc surface of the movable seat 91. The counterweight bar 12 is arranged in conjunction with the screw rod 94. The counterweight bar 12 improves the uniformity of weight distribution around the central axis 2 and balances the weight of the screw rod 94, nut 92 and screw rod seat 93.

[0034] A method for using a melanocyte dissolution and separation device comprises the following steps:

[0035] (S1: Adjust the depth of the test tube holder 4: Press the lower end of the card plate 53 with two fingers. The card plate 53 rotates around the pin 52 and its upper end tilts up. The upper end of the card plate 53 moves out of the gear slot 54 while squeezing the spring piece 55. Then, pinch the two card plates 53 to drive the base 51 to slide along the gear slot 54. Release the card plate 53. The upper end of the card plate 53 is movably engaged with the horizontal groove of the gear slot 54.

[0036] (S2: Adjust the angle of the test tube holder 4: Rotate the screw 94, which drives the movable seat 91 to move up and down along the central axis 2 through the nut 92. The movable seat 91 drives the rack 8 to slide vertically along the support seat 16 through the support bar 95. The rack 8 drives the test tube holder 4 to rotate through the gear 7 and the rotating shaft 6. The test tube holder 4 drives the dial 14 to rotate around the axis of the rotating shaft 6. The angle between the test tube holder 4 and the central axis 2 is between 20 and 45 degrees;

[0037] (S3: Insert the test tube: Insert the test tube containing the melanocyte solution into the test tube holder 4. The bottom of the test tube contacts the base 51. Under the action of the spring 55, the clamping plate 53 clamps the test tube through the rubber strip 56.

[0038] (S4: Centrifugal precipitation: Adjust the control switch 10, the motor 11 is running, the output shaft of the motor 11 drives the central shaft 2 to rotate, and the central shaft 2 drives the tilted test tube to rotate through the support base 16, the rotating shaft 6 and the test tube holder 4, and rotates for three to five minutes at a speed of 1000 revolutions per minute;

[0039] (S5: Pour out the supernatant: Rotate the screw 94 to control the rack 8 to move downward, driving the lower end of the test tube holder 4 to rotate upward, and the supernatant on the upper layer of the test tube flows out from the port and into the supernatant collection tube. After the supernatant is discharged, rotate the screw 94 in the opposite direction to control the rack 8 to move upward, and then remove the test tube.

[0040] The working principle of a melanocyte dissolution and separation device and its use method provided by the present invention is as follows: open the upper cover of the chassis 1, and adjust the position of the base 51 inside the test tube holder 4 according to the length of the centrifugal test tube. When the test tube is short, use two fingers to press the lower end of the card plate 53, and the card plate 53 rotates around the pin shaft 52 to tilt its upper end. The upper end of the card plate 53 moves out of the gear slot 54 while squeezing the spring sheet 55, and then pinch the two card plates 53 to drive the base 51 to slide along the gear slot 54. The gear slot 54 consists of a vertical slot parallel to the axis of the test tube holder 4 and a plurality of horizontal slots perpendicular to the axis. When it moves to the appropriate position, the upper end of the card plate 53 The end of the card plate 53 corresponds to the horizontal groove of the gear slot 54, and then the card plate 53 is released. The elastically deformed spring sheet 55 presses the card plate 53 down to reset. The upper end of the card plate 53 is movably engaged with the horizontal groove of the gear slot 54. The end of the card plate 53 and the rubber strip 56 extend into the interior of the test tube holder 4. Then, the screw rod 94 is rotated by the hand wheel. The screw rod 94 drives the movable seat 91 to move up and down along the central axis 2 through the nut 92. The movable seat 91 drives the rack 8 to slide vertically along the support seat 16 through the support bar 95. The rack 8 drives the test tube holder 4 to rotate through the gear 7 and the rotating shaft 6. The test tube holder 4 drives the dial 14 to rotate around the axis of the rotating shaft 6, so that the scale can be viewed. The positions of the dial 14 and the pointer 13 determine the angle of the test tube holder 4, and then determine the centrifugal angle of the test tube. After the adjustment is completed, the test tube containing the melanocyte solution is inserted into the test tube holder 4. The bottom of the test tube contacts the base 51. At the same time, under the action of the spring sheet 55, the clamping plate 53 clamps the test tube through the rubber strip 56, thereby fixing the test tube. The upper cover of the chassis 1 is closed, and then the control switch 10 is adjusted. The motor 11 is turned on. The output shaft of the motor 11 drives the central shaft 2 to rotate. The central shaft 2 drives the tilted test tube to rotate through the support base 16, the rotating shaft 6 and the test tube holder 4, and the sedimentation of different cell components under the action of centrifugal force is utilized. The speed difference is used to achieve separation. After the motor 11 has run for a period of time, the motor 11 stops running, the upper cover of the chassis 1 is opened, and then the supernatant collecting tube is placed inside the placement slot 3. The sealing plug on the head of the test tube is removed, and the screw rod 94 is rotated to control the rack 8 to move downward, driving the lower end of the test tube holder 4 to rotate upward, and the internal test tube will rotate to a horizontal state. The supernatant on the upper layer of the test tube flows out from the port and flows into the supernatant collecting tube. After the supernatant is discharged, the screw rod 94 is rotated in the opposite direction to control the rack 8 to move upward to avoid the discharge of the precipitate. Then the test tube can be taken out, and the precipitate part is the required melanocytes, thereby completing the dissolution and separation of the melanocytes.

[0041] It is worth noting that the motor 11 disclosed in the above embodiment can be freely configured according to the actual application scenario. The motor 11 can use a brushless motor of model 57BLF. The control switch 10 is provided with a switch button corresponding to the motor 11 for controlling its switching operation.

[0042] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention description and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. A melanocyte dissolution and separation device, characterized in that: It comprises a chassis (1) and a lifting assembly (9); The chassis (1) is rotatably connected to a central axis (2) inside the chassis, a placement groove (3) is provided at the upper end of the central axis (2), uniformly distributed support seats (16) are provided at the upper end of the outer arc surface of the central axis (2), a plurality of support seats (16) are rotatably connected to a rotating shaft (6) at one end away from the central axis (2), a test tube seat (4) is provided outside the plurality of rotating shafts (6), an adjustable tube seat assembly (5) is movably connected to the bottom of the test tube seat (4), a gear (7) is provided in the middle of the outer arc surface of the plurality of rotating shafts (6), a rack (8) is slidably connected inside the plurality of support seats (16), and the rack (8) and the gear (7) inside the same support seat (16) are meshingly connected; Lifting assembly (9): used to control the up and down movement of the plurality of racks (8).

2. A melanocyte dissolution and separation device according to claim 1, characterized in that: The lifting assembly (9) comprises a moving seat (91), a nut (92), a screw seat (93), a screw (94) and a support bar (95); the moving seat (91) is slidably connected to the middle of the outer arc surface of the central shaft (2); uniformly distributed support bars (95) are arranged around the outer arc surface of the moving seat (91); the upper ends of the plurality of support bars (95) are fixedly connected to the vertically corresponding racks (8); two screw seats (93) are arranged in the middle of the outer arc surface of the central shaft (2); a screw (94) is rotatably connected between the two screw seats (93); a nut (92) is arranged on the outer arc surface of the moving seat (91); the nut (92) is threadedly connected to the screw (94).

3. A melanocyte dissolution and separation device according to claim 2, characterized in that: A counterweight bar (12) is provided on the outer arc surface of the movable seat (91), and the counterweight bar (12) is arranged in cooperation with the screw rod (94).

4. The melanocyte dissolution and separation device according to claim 1, characterized in that: It also comprises a pointer (13) and a dial (14), wherein the dial (14) is respectively arranged at the upper end of the test tube holder (4), and the pointer (13) is respectively arranged at the side of the support holder (16), and the pointer (13) and the dial (14) are arranged in coordination.

5. The melanocyte dissolution and separation device according to claim 1, characterized in that: It also includes positioning plates (15), which are respectively arranged on the upper ends of the racks (8).

6. The melanocyte dissolution and separation device according to claim 1, characterized in that: The adjustable tube seat assembly (5) comprises a base (51), a pin shaft (52), a clamping plate (53), a gear slot (54) and a spring sheet (55); the bases (51) are respectively slidably connected to the inside of the test tube seats (4); the outer arc surfaces of the test tube seats (4) are provided with symmetrically distributed gear slots (54); the upper ends of the bases (51) are provided with two pin shafts (52); the outer arc surfaces of the pin shafts (52) are rotatably connected with the clamping plate (53); the upper ends of the bases (51) are provided with two spring sheets (55); the spring sheets (55) and the gear slots (54) are arranged in cooperation with the clamping plate (53).

7. The melanocyte dissolution and separation device according to claim 6, characterized in that: The adjustable tube seat assembly (5) further comprises a rubber strip (56), wherein the rubber strip (56) is respectively arranged at one end of the clamping plate (53) extending to the inside of the test tube seat (4).

8. The melanocyte dissolution and separation device according to claim 1, characterized in that: A control switch (10) is provided on the left side of the upper surface of the chassis (1), a motor (11) is provided at the lower end inside the chassis (1), an output shaft of the motor (11) is fixedly connected to the lower end of the central shaft (2), an input end of the motor (11) is electrically connected to the output end of the control switch (10), and an input end of the control switch (10) is electrically connected to an external power supply.

9. A method for using a melanocyte lysis and separation device, characterized in that: A melanocyte dissolution and separation device according to any one of claims 1 to 8 is used, comprising the following steps: (S1: Adjust the depth of the test tube holder (4): Press the lower end of the card plate (53) with two fingers, the card plate (53) rotates around the pin shaft (52) and its upper end tilts up, the upper end of the card plate (53) moves out of the gear slot (54) and squeezes the spring sheet (55) at the same time, then pinch the two card plates (53) to drive the base (51) to slide along the gear slot (54), release the card plate (53), and the upper end of the card plate (53) is movably engaged with the horizontal slot of the gear slot (54); (S2: adjusting the angle of the test tube holder (4): rotating the screw rod (94), the screw rod (94) drives the movable seat (91) to move up and down along the central axis (2) through the nut (92), the movable seat (91) drives the rack (8) to slide vertically along the support seat (16) through the support bar (95), the rack (8) drives the test tube holder (4) to rotate through the gear (7) and the rotating shaft (6), and the angle between the test tube holder (4) and the central axis (2) is between 20 and 45 degrees; (S3: inserting the test tube: inserting the test tube containing the melanocyte solution into the test tube holder (4), the bottom of the test tube contacts the base (51), and under the action of the spring sheet (55), the clamping plate (53) clamps the test tube through the rubber strip (56); (S4: Centrifugal sedimentation: The control switch (10) is adjusted, and the motor (11) is operated. The output shaft of the motor (11) drives the central shaft (2) to rotate. The central shaft (2) drives the inclined test tube to rotate through the support base (16), the rotating shaft (6) and the test tube base (4). The rotation lasts for three to five minutes; (S5: pour out the supernatant: rotate the screw (94) to control the rack (8) to move downward, drive the lower end of the test tube holder (4) to rotate upward, and the supernatant on the upper layer of the test tube flows out from the port and flows into the supernatant collection tube. After the supernatant is discharged, rotate the screw (94) in the opposite direction to control the rack (8) to move upward, and then take out the test tube.

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