Device and method for purifying and preparing cattle spleen peptide for enhancing immunity

Abstract

The invention belongs to the technical field of cattle spleen peptide preparation, and particularly relates to an immunity-enhancing cattle spleen peptide purification preparation device and method.The immunity-enhancing cattle spleen peptide purification preparation device comprises a tank body, a shaft pipe is rotationally installed in the tank body, and the tank body communicates with at least two feeding pipes; at least two groups and a plurality of fan-shaped plates are mounted on the shaft tube, and the fan-shaped plates are connected with a lifting butt joint mechanism mounted on the shaft tube; a filtrate net is mounted in the tank body, a filtrate cavity is formed between the filtrate net and the tank body, and a sealed flow guide mechanism is arranged at the bottom of the filtrate net; pollution generated when slurry subjected to enzyme deactivation is discharged out of the tank body for filtering treatment can be reduced, meanwhile, protease can be continuously added or crushing treatment can be carried out on impurities left in the tank body after filter pressing according to needs, and therefore the utilization rate of cattle spleen peptide raw materials can be increased.

Description

Technical Field

[0001] The invention belongs to the technical field of bovine spleen peptide preparation, and particularly relates to a bovine spleen peptide purification and preparation device and method for enhancing immunity. Background Art

[0002] Bovine spleen peptide, a natural bioactive substance derived from bovine spleen, is well-known for its unique immunomodulatory effect. It contains a variety of amino acids, trace elements and active peptide ingredients, which can significantly enhance the human immune system. Bovine spleen peptide can not only enhance immunity but also improve digestive function by promoting the production of cytokines and regulating immune balance.

[0003] The existing purification and preparation of bovine spleen peptide mostly requires first putting the crushed and homogenized bovine spleen peptide into a mixing tank, then adding an appropriate amount of protease into the mixing tank for mixing, allowing the protease to hydrolyze the bovine spleen peptide slurry, and then separating and purifying the hydrolyzed and enzyme-inactivated bovine spleen peptide slurry.

[0004] The existing purification and preparation device can only stir and mix the bovine spleen peptide slurry and protease, and then directly discharge the slurry after enzymatic hydrolysis and enzyme inactivation for separation and purification. Since the mixed slurry after enzymatic hydrolysis contains impurities such as un-enzymatically hydrolyzed proteins, cell fragments and fat, if it is directly separated and purified, it will affect the quality of the purified bovine spleen peptide. If the mixed slurry after enzymatic hydrolysis is discharged from the mixing tank for filtration, it is necessary to put the un-enzymatically hydrolyzed proteins, cell fragments and fat impurities after filtration into the mixing tank again for enzymatic hydrolysis and mixing reaction, which will reduce the efficiency of the purification and preparation of bovine spleen peptide. Summary of the invention

[0005] To solve the above technical problems, the present invention is achieved through the following technical solutions: The present invention discloses a device and method for purifying and preparing bovine spleen peptide for enhancing immunity, comprising a tank body, an axle tube rotatably installed inside the tank body, and at least two feed pipes connected to the tank body; at least two groups and multiple fan-shaped plates are installed on the axle tube, and the fan-shaped plates are connected to a lifting docking mechanism installed on the axle tube; a filtrate net is installed inside the tank body, and a filtrate cavity is formed between the filtrate net and the tank body, and a sealing guide mechanism is arranged at the bottom of the filtrate net.

[0006] Furthermore, the lifting and docking mechanism includes a screw member, which is rotatably inserted into the shaft tube through a bearing, and two sleeves are sleeved on the screw member through two ball nuts, and the multiple plug-in blocks on the lower surface of the upper sleeve and the multiple plug-in blocks on the upper surface of the lower sleeve are staggered with each other, and a plurality of vertical guide grooves are opened in a circumferential array on the shaft tube, the outer side surface of each plug-in block is connected to a connecting block, and each connecting block is slidably arranged in the vertical guide groove, the outer end of each connecting block is respectively connected to a fan-shaped plate, and the outer end of each fan-shaped plate is fitted with the inner wall of the tank, and the ball nut and the sleeve are connected by an electromagnetic adsorption component.

[0007] Furthermore, the electromagnetic adsorption component includes an electromagnetic ring, an annular cavity is opened inside the sleeve, and the electromagnetic ring is insulated and installed in the annular cavity, the outer ring surface of the ball nut is insulated and provided with a metal ring, and the electromagnetic ring is sleeved outside the metal ring, and multiple electromagnetic suction blocks are insulated and installed at intervals on the groove walls on both sides of the vertical guide groove, and the side walls of the connecting block and the vertical guide groove are insulated and provided with metal sheets, and multiple elastic sealing strips are segmented in the vertical guide groove, and the ends of the elastic sealing strips are respectively connected to the connecting block and the upper and lower groove bottoms of the vertical guide groove.

[0008] Furthermore, the two groups of filtrate nets are connected to the shaft tube through bearings, and the sealing guide mechanism includes a sealing disk, which is slidably and sealingly sleeved on the shaft tube, and the sealing disk is attached to the lower surface of the filtrate net below. At least two electric push rods are fixedly provided at the bottom of the tank body, and the output rods of the electric push rods are connected to the sealing disk. A vortex groove is formed on the upper surface of the sealing disk, and a vortex hose is coiled in the vortex groove, and a seepage hole is opened on the vortex hose, and the free pipe mouth of the guide hose is connected to the drainage conduit arranged on the tank body through a connecting hose, and the top of the tank body is connected to a drainage elbow, and the bottom pipe mouth of the drainage elbow is located on the upper filtrate net surface.

[0009] Furthermore, a shearing assembly is provided on the fan-shaped plate, and the shearing assembly includes a square mesh plate. A through square hole is penetrated on the fan-shaped plate, and a square mesh plate is sealed and inserted in the through square hole, and a recessed square hole is provided at the outer hole opening of the through square hole. Square strips are fixed at the upper and lower ends of the square mesh plate, and the square strips are connected to the bottom of the recessed square hole through a spring part, and the size of the square strips is the same as the size of the recessed square hole.

[0010] Furthermore, the bottom end of the shaft tube extends out of the tank body and is connected to a fixed servo motor through a gear assembly, the bottom end of the screw member extends out of the shaft tube and is connected to a fixed servo motor, and the top end of the shaft tube is in a sealed state and is rotatably connected to the filtrate net above.

[0011] Furthermore, a waste discharge pipe is connected to the side wall of the tank body, and the waste discharge pipe is located on the upper surface of the filtrate net.

[0012] A method for purifying and preparing bovine spleen peptide for enhancing immunity, the method is applicable to the above-mentioned bovine spleen peptide purification and preparation device, and the method comprises the following steps: S1: Raw material preparation: Select high-quality beef spleen as raw material, clean and chop it for pretreatment; S2: Enzymatic hydrolysis: Mix the pretreated bovine spleen with water or buffer, put it into the tank through the feeding tube, add an appropriate amount of protease, and perform enzymatic hydrolysis under appropriate temperature and pH conditions to hydrolyze the protein into peptides; S3: Filtration: Remove impurities in the mixed slurry through the cooperation of the sealing pressure plate and the filtrate net formed in the tank body; S4: Separation and purification: Use ultrafiltration or membrane separation technology to separate the peptides in the enzymatic hydrolysate; use chromatography technology to further separate and purify the peptides; S5: Concentration and drying: Concentrate the purified peptide solution to remove excess solvent; freeze-dry or spray-dry the concentrate to form a powdered bovine spleen peptide product.

[0013] The present invention has the following beneficial effects: 1. The present invention provides a filtrate net in the tank body, and the filtrate net is supported and sealed by a sealing guide mechanism. When two groups and multiple fan-shaped plates are separated, they can be driven by a shaft tube to rotate in the tank body, so as to achieve stirring and mixing of the bovine spleen peptide slurry and the added protease in the tank body. When the upper and lower groups and multiple fan-shaped plates are aligned with each other to form a sealing pressure plate, the sliding sealing pressure plate can directly perform filter pressing on the stirred and mixed slurry through the filtrate net, which can reduce the pollution generated when the slurry after enzyme inactivation is discharged from the tank body for filtering. At the same time, the impurities remaining in the tank body after filter pressing can continue to be added with protease or crushed as needed, thereby improving the utilization rate of the bovine spleen peptide raw material.

[0014] 2. In the present invention, a plurality of plug-in blocks are staggeredly installed on the corresponding end faces of the upper and lower sleeves, and the outer side surface of each plug-in block is connected to a fan-shaped plate through a connecting block. When the two sleeves are fitted together, the plurality of plug-in blocks will be plugged into each other to form a circular ring, and the plurality of fan-shaped plates will form a ring-shaped sealing pressure plate. The rotation of the screw member will drive it to move up and down to perform filter pressure treatment on the mixed slurry. When the ring-shaped sealing pressure plate is separated, the upper and lower groups and the plurality of fan-shaped plates are staggered in the tank body, thereby improving the sufficient stirring and mixing of the slurry and the protease.

[0015] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0017] Figure 1 It is a schematic diagram of the overall structure of the embodiment disclosed in the present invention; Figure 2 A schematic diagram of the internal structure of a tank according to an embodiment of the present invention; Figure 3 It is a schematic structural diagram of two groups of fan-shaped plates according to an embodiment disclosed in the present invention; Figure 4 It is a structural schematic diagram of the lifting and docking mechanism of the disclosed embodiment of the present invention; Figure 5 A cross-sectional view of a sleeve according to an embodiment of the present invention; Figure 6 It is a structural schematic diagram of a sealing disk according to an embodiment of the present invention; Figure 7 An exploded view of a shear assembly and a sector plate according to an embodiment of the present invention.

[0018] In the figure: 1. tank body; 2. Axis tube; 21. Vertical guide groove; 3. Feed pipe; 4. Sector plate; 41. Through square hole; 42. Concave square hole; 5. Lifting docking mechanism; 51. Screw piece; 52. Sleeve; 53. Plug-in block; 54. Connecting block; 55. Electromagnetic ring; 56. Metal ring; 57. Electromagnetic suction block; 58. Metal sheet; 59. Elastic sealing strip; 6. Filtrate screen; 7. Sealing guide mechanism; 71. Sealing disk; 711. Vortex groove; 72. Electric push rod; 73. Vortex hose; 74. Connecting hose; 75. Drainage conduit; 76. Drainage elbow; 8. Shearing assembly; 81. Square mesh plate; 82. Square strip; 83. Spring member; 9. Gear assembly; 10. Waste discharge pipe. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] In the description of the present invention, it should be understood that the terms "opening", "upper", "lower", "thickness", "top", "middle", "length", "inside", "all around" and the like indicating orientation or positional relationship are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the components or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0021] See also Figure 1-Figure 7 As shown, the present invention is a bovine spleen peptide purification and preparation device and method for enhancing immunity, comprising a tank body 1, a shaft tube 2 is rotatably installed inside the tank body 1, and at least two feed pipes 3 are connected to the tank body 1; at least two groups and multiple fan-shaped plates 4 are installed on the shaft tube 2, and the fan-shaped plates 4 are connected to a lifting docking mechanism 5 installed on the shaft tube 2; a filtrate net 6 is installed inside the tank body 1, and a filtrate cavity is formed between the filtrate net 6 and the tank body 1, and a sealing guide mechanism 7 is provided at the bottom of the filtrate net 6; Preferably, the bottom end of the shaft tube 2 extends out of the tank body 1 and is connected to the fixed servo motor through the gear assembly 9, the bottom end of the lead screw 51 extends out of the shaft tube 2 and is connected to the fixed servo motor, and the top end of the shaft tube 2 is in a sealed state and is rotatably connected to the filtrate net 6 above; Specifically, the present invention installs an axis tube 2 in a tank body 1, and two groups of preferably four fan-shaped plates 4 are slidably arranged on the axis tube 2 in a circular array. When it is necessary to perform an enzymatic hydrolysis reaction on the crushed and homogenized bovine spleen peptide slurry, the slurry is first put into the tank body 1 through a feeding tube 3, and then an appropriate amount of protease is put into the tank body 1 through another feeding tube 3, and then the servo motor fixed at the bottom of the tank body 1 is started. The servo motor drives the axis tube 2 to rotate through a double-meshing gear assembly 9, so that the multiple fan-shaped plates 4 rotate in the tank body 1 to fully stir the put-in slurry and protease, so that the protease can fully enzymatically hydrolyze the bovine spleen peptide. After the enzymatic hydrolysis reaction is completed, the enzymatically hydrolyzed slurry in the tank body 1 is then inactivated by the heating element on the tank body 1. When it is necessary to discharge the inactivated slurry, the multiple fan-shaped plates 4 below are controlled to move upward through the lifting and docking mechanism 5. The lifting and docking mechanism 5 drives the formed sealing plate to move toward the filtrate net 6, so that the enzyme-inactivated slurry can be subjected to filter pressing treatment, so that the impurities such as unhydrolyzed proteins, cell fragments and fats in the enzyme-inactivated slurry will be directly retained in the tank body 1 for subsequent treatment, and the setting of the sealing guide mechanism 7 can block the filtrate net 6 to prevent the slurry in the tank body 1 from leaking when the slurry is stirred and mixed with the protease. The mixed enzyme-inactivated slurry is directly subjected to filter pressing treatment in the tank body 1, which can reduce the pollution generated when the enzyme-inactivated slurry is discharged from the tank body 1 for filtration treatment. At the same time, the impurities retained in the tank body 1 after filter pressing can continue to be added with protease or crushed as needed, thereby improving the utilization rate of the bovine spleen peptide raw material.

[0022] In the design of the present invention, the lifting docking mechanism 5 includes a screw member 51, which is inserted into the shaft tube 2 through a bearing, and two sleeves 52 are sleeved on the screw member 51 through two ball nuts, and a plurality of plug-in blocks 53 on the lower surface of the upper sleeve 52 and a plurality of plug-in blocks 53 on the upper surface of the lower sleeve 52 are staggered with each other, and a plurality of vertical guide grooves 21 are provided in a circumferential array on the shaft tube 2, and the outer side surface of each plug-in block 53 is connected to a connecting block 54, and each connecting block 54 is slidably arranged in the vertical guide groove 21, and the outer end of each connecting block 54 is respectively connected to a fan-shaped plate 4, and the outer end of each fan-shaped plate 4 is in contact with the inner wall of the tank body 1, and the ball nut and the sleeve 52 are connected through an electromagnetic adsorption component; Specifically, it is preferred to set four plug-in blocks 53 on the corresponding end faces of the upper and lower sleeves 52, and the plug-in blocks 53 on the two sleeves 52 are staggered, and each group of sector plates 4 is also preferably four, and the circumferential array on the shaft tube 2 is provided with eight non-interfering vertical guide grooves 21. Therefore, when the four sector plates 4 at the bottom need to slide upward to connect with the lead screw sector plates 4 at the top, the electromagnetic adsorption component on the upper sleeve 52 is first controlled to disengage from the internal ball nut, and at this time, the multiple upper connection blocks 54 will be fixed in the vertical guide groove 21, and then the servo motor connected to the bottom of the lead screw member 51 extending from the bottom of the shaft tube 2 will be controlled to work, so that the lead screw member 51 The shaft tube 2 rotates in the shaft tube 2, and the shaft tube 2 is in a fixed state at this time, and then the sleeve 52 located below will slide upward under the drive of the ball nut, and the multiple plug-in blocks 53 will drive the four lower sector plates 4 to move upward through the multiple connecting blocks 54. When the four lower plug-in blocks 53 are inserted between the four upper plug-in blocks 53, the four connecting blocks 54 sliding upward will be flush with the four upper connecting blocks 54, and then the four upward sliding sector plates 4 will be sealed and inserted between the two adjacent sector plates 4 above, so that the eight mutually fitting sector plates 4 form an annular sealing pressure plate in the tank body 1, and then the two ball nuts on the screw member 51 and the two sleeves are engaged. 52 are connected at the same time, and the rotation of the lead screw 51 can drive the annular sealing pressure plate to move downward or upward, and push the enzymatically hydrolyzed slurry above or below the sealing pressure plate upward or downward, and then the slurry will pass through the filtrate net 6 for pressure filtration, and the remaining impurities will be retained on the filtrate net 6, and then it is necessary to discharge the enzymatically hydrolyzed and enzyme-inactivated slurry from the outside of the tank body 1 for filtration treatment, which can not only prevent the pollution of the enzymatically hydrolyzed slurry during filtration, but also make full use of the impurities remaining after filtration, thereby improving the effect of bovine spleen peptide purification and preparation, and when the enzymatically hydrolyzed slurry is pressure filtered, at this time, one of the sleeves 52 is controlled In a fixed state, the rotation of the screw member 51 will drive the other sleeve 52 to slide downward or upward, thereby separating the mutually fitted sector plates 4, allowing the shaft tube 2 to continue to rotate, and driving the separated two groups of multiple sector plates 4 to rotate in the tank body 1 to stir and mix the subsequently added slurry and protease. At the same time, the sleeve 52 can also be driven to slide by the screw member 51 to adjust the distance between the upper and lower groups of multiple sector plates 4, thereby improving the sufficient stirring and mixing of the slurry and protease. When the shaft tube 2 rotates, the sleeve 52 will be disconnected from the ball nut, so that it will not interfere with the normal rotation of the multiple sector plates 4.

[0023] In the design of the present invention, the electromagnetic adsorption component includes an electromagnetic ring 55, an annular cavity is opened inside the sleeve 52, and the electromagnetic ring 55 is insulated and installed in the annular cavity, and the outer ring surface of the ball nut is insulated and provided with a metal ring 56, and the electromagnetic ring 55 is sleeved outside the metal ring 56, and multiple electromagnetic adsorption blocks 57 are insulated and installed on the groove walls on both sides of the vertical guide groove 21. The side wall of the connecting block 54 that is in contact with the vertical guide groove 21 is insulated and provided with a metal sheet 58, and multiple elastic sealing strips 59 are arranged in sections in the vertical guide groove 21, and the ends of the elastic sealing strips 59 are respectively connected to the connecting block 54 and the upper and lower groove bottoms of the vertical guide groove 21; Specifically, when the ball nut needs to be disconnected from the sleeve 52, the electromagnetic suction block 57 on the inner ring surface of the electromagnetic ring 55 is controlled to be powered off, so as to disconnect the metal ring 56, and the metal sheet 58 on the side of the connecting block 54 will be fixed to the vertical guide groove 21 through the magnetic adsorption of the electromagnetic suction block 57 after being energized, so that the rotation of the screw member 51 will only drive the ball nut to rotate in the unconnected sleeve 52; and when the ball nut needs to be connected to the sleeve 52, the electromagnetic suction block 57 on the inner ring surface of the electromagnetic ring 55 is controlled to be energized, so that it adsorbs and fixes the metal ring 56, and the groove wall of the vertical guide groove 21 is in contact with the metal sheet 58. The electromagnetic suction block 57 is in a power-off state, and the rotation of the screw member 51 will drive the connected sleeve 52 to slide up and down in the shaft tube 2 through the ball nut, and at this time the connecting block 54 will slide up and down in the vertical guide groove 21, thereby driving multiple fan-shaped plates 4 to slide up and down in the tank body 1, and the elastic sealing strip 59 provided can seal the vertical guide groove 21 to prevent the mixed slurry in the tank body 1 from entering the shaft tube 2 and causing unnecessary waste. When the connecting block 54 slides in the vertical guide groove 21, the elastic sealing strip 59 will be compressed or stretched and deformed, so that it will not interfere with the normal sliding of the connecting block 54.

[0024] In the design of the present invention, the two groups of filtrate nets 6 are connected to the shaft tube 2 through bearings, and the sealing guide mechanism 7 includes a sealing disk 71, which is slidably and sealingly sleeved on the shaft tube 2, and the sealing disk 71 is attached to the lower surface of the filtrate net 6 below. At least two electric push rods 72 are fixedly provided at the bottom of the tank body 1, and the output rods of the electric push rods 72 are connected to the sealing disk 71. A vortex groove 711 is formed on the upper surface of the sealing disk 71, and a vortex hose 73 is coiled in the vortex groove 711, and a seepage hole is opened on the vortex hose 73, and the free pipe mouth of the guide hose is connected to the drainage conduit 75 provided on the tank body 1 through a connecting hose 74, and a drainage elbow 76 is connected to the top of the tank body 1, and the bottom end pipe mouth of the drainage elbow 76 is located on the surface of the filtrate net 6 above; Specifically, two groups of filtrate nets 6 are arranged in the tank body 1, and the two groups of filtrate nets 6 are respectively located at the two ends of the tank body 1, so that when the sealed pressure plate formed by the sealing pressure plate slides upward, the mixed slurry above can be pushed upward, so that the slurry is filtered through the filtrate net 6 above, and the filtrate of the filtration is discharged through the upper drainage elbow 76. When the sealed pressure plate formed by the sealing pressure plate moves downward, the output rod of the electric push rod 72 is controlled to be recovered, so that it drives the sealing disk 71 to move downward. The vortex hose 73 retracted into the vortex groove 711 will open, and because the other end of the connecting hose 74 passes downward through the sealing disk 71, when the sealing pressure plate moves downward, the mixed slurry below will be pressed downward, so that the filtrate flows into the sealing disk 71 through the filtrate net 6, and then penetrates into the vortex hose 73 through the opened seepage hole, and the suction pump connected to the drainage conduit 75 will suck and discharge the filtrate extending into the vortex hose 73 through the connecting hose 74, so that the filtrate can be discharged. The filtrate of the filtration is quickly discharged and collected; and when it is necessary to seal the filtrate net 6 below, the output rod of the electric push rod 72 is controlled to extend, so that it pushes the sealing disk 71 to move upward and fit to the lower surface of the filtrate net 6 below. At this time, the vortex hose 73 will be squeezed and trapped in the vortex groove 711, so that there is no gap between the sealing disk 71 and the filtrate net 6, so that the tank body 1 can continue to stir and mix the bovine spleen peptide slurry and protease, and the feed pipe 3 is located below the upper filtrate net 6. Therefore, the liquid level of the slurry stirred and mixed in the tank body 1 will not be higher than the upper filtrate net 6, and the upper filtrate net 6 will not affect the stirring and mixing of the bovine spleen peptide slurry and protease; and when there are cell fragments that are not completely broken on the upper surface of the lower filtrate net 6, the sealing pressure plate formed at this time will rotate on the upper surface of the lower filtrate net 6 driven by the shaft tube 2, which can realize the grinding and crushing of the unbroken cell fragments, thereby further improving the utilization rate of the bovine spleen peptide raw material.

[0025] In the design of the present invention, a shearing assembly 8 is provided on the sector plate 4, and the shearing assembly 8 includes a square mesh plate 81. A through square hole 41 is formed on the sector plate 4, and a square mesh plate 81 is sealed and inserted in the through square hole 41, and a recessed square hole 42 is formed at the outer opening of the through square hole 41. A rectangular strip 82 is fixed at the upper and lower ends of the square mesh plate 81, and the rectangular strip 82 is connected to the bottom of the recessed square hole 42 through a spring member 83, and the size of the square strip 82 is the same as that of the recessed square hole 42. Specifically, in order to further improve the mixing effect of bovine spleen peptide slurry and protease, a vertical square mesh plate 81 is slidably arranged on the fan-shaped plate 4. Therefore, when the fan-shaped plate 4 rotates, the square mesh plate 81 will generate an axial shear force on the slurry in the tank body 1, thereby further accelerating the enzymatic hydrolysis effect of the protease and the slurry. When the eight fan-shaped plates 4 are fitted together on the side surfaces, the annular sealing plate slides up and down in the tank body 1 to filter the mixed slurry, and as the sealing plate continuously slides toward the surface of the filtrate net 6, the square strips 82 located on the filtrate net 6 will contact the filtrate net 6, and the square strips 82 will be The square mesh plate 81 is pushed toward the back of the sealing pressure plate, and the spring member 83 is compressed at this time. The thickness of the rectangular strip 82 is less than the thickness of the recessed square hole 42, so that the rectangular strip 82 can be embedded in the recessed square hole 42, thereby facilitating the sealing pressure plate to contact the filtrate mesh 6 in a similar fitting state, thereby grinding and crushing the cell fragments remaining on the surface of the filtrate mesh 6. Since the mesh diameter of the square mesh plate 81 is smaller than the thickness of the fan-shaped plate 4, when the sealing pressure plate slides, the mixed slurry in the sliding direction will not leak through the mesh holes of the square mesh plate 81, thereby improving the filtration effect of the mixed slurry.

[0026] In the design of the present invention, the side wall of the tank body 1 is connected to a waste discharge pipe 10, and the waste discharge pipe 10 is located on the upper surface of the filtrate net 6; specifically, when it is necessary to discharge the residual fat and cell fragments that cannot be ground and broken in the tank body 1, the sealing disk 71 is controlled to slide downward a small distance to release and open the vortex hose 73, and then the cleaning liquid is injected into the top of the sealing disk 71 through the drainage duct 75, the connecting hose 74 and the vortex hose 73, and then the cleaning liquid will penetrate into the top of the filtrate net 6, so that the cleaning liquid can flush the residual debris, and then be discharged through the opened waste discharge pipe 10. At the same time, this method can also be used to clean the inside of the tank body 1.

[0027] A method for purifying and preparing bovine spleen peptide for enhancing immunity, the method is applicable to the above-mentioned bovine spleen peptide purification and preparation device, and the method comprises the following steps: S1: Raw material preparation: Select high-quality beef spleen as raw material, clean and chop it for pretreatment; S2: Enzymatic hydrolysis: the pre-treated bovine spleen is mixed with water or buffer, and is put into the tank 1 through the feed pipe 3, and an appropriate amount of protease (such as trypsin, papain, flavor protease, etc.) is added to perform enzymatic hydrolysis under appropriate temperature and pH conditions to hydrolyze the protein into peptide segments; S3: Filtration: Impurities in the mixed slurry are removed through the cooperation of the sealing pressure plate formed in the tank body 1 and the filtrate net 6; S4: Separation and purification: Use ultrafiltration or membrane separation technology to separate the peptides in the enzymatic hydrolysate; use chromatography technology (such as gel chromatography, ion exchange chromatography, reversed-phase high-performance liquid chromatography, etc.) to further separate and purify the peptides; S5: Concentration and drying: Concentrate the purified peptide solution to remove excess solvent; freeze-dry or spray-dry the concentrate to form a powdered bovine spleen peptide product.

[0028] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0029] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A bovine spleen peptide purification and preparation device for enhancing immunity, characterized in that: It comprises a tank body, an axle tube is rotatably mounted inside the tank body, and at least two feed pipes are connected to the tank body; At least two groups and multiple fan-shaped plates are installed on the shaft tube, and the fan-shaped plates are connected to the lifting and docking mechanism installed on the shaft tube; A filtrate net is installed inside the tank body, and a filtrate cavity is formed between the filtrate net and the tank body, and a sealing guide mechanism is arranged at the bottom of the filtrate net.

2. The device for purifying and preparing bovine spleen peptide for enhancing immunity according to claim 1, characterized in that: The lifting and docking mechanism includes a screw member, which is inserted into the shaft tube through a bearing and rotates, and two sleeves are sleeved on the screw member through two ball nuts, and a plurality of plug-in blocks on the lower surface of the upper sleeve and a plurality of plug-in blocks on the upper surface of the lower sleeve are staggered with each other, and a plurality of vertical guide grooves are opened in a circular array on the shaft tube, and the outer side surface of each plug-in block is connected to a connecting block, and each connecting block is slidably arranged in the vertical guide groove, and the outer end of each connecting block is respectively connected to a fan-shaped plate, and the outer end of each fan-shaped plate is fitted with the inner wall of the tank body, and the ball nut and the sleeve are connected by an electromagnetic adsorption component.

3. The device for purifying and preparing bovine spleen peptide for enhancing immunity according to claim 2, characterized in that: The electromagnetic adsorption component includes an electromagnetic ring, an annular cavity is opened inside the sleeve, and the electromagnetic ring is insulated and installed in the annular cavity, a metal ring is insulated and arranged on the outer ring surface of the ball nut, and the electromagnetic ring is sleeved outside the metal ring, a plurality of electromagnetic suction blocks are insulated and installed on the groove walls on both sides of the vertical guide groove at intervals, a metal sheet is insulated and arranged on the side walls where the connecting block and the vertical guide groove are in contact, a plurality of elastic sealing strips are arranged in sections in the vertical guide groove, and the ends of the elastic sealing strips are respectively connected to the connecting block and the upper and lower groove bottoms of the vertical guide groove.

4. The device for purifying and preparing bovine spleen peptide for enhancing immunity according to claim 1, characterized in that: The two groups of filtrate nets are connected to the shaft tube through bearings, and the sealing guide mechanism includes a sealing disk, which is slidably and sealingly sleeved on the shaft tube, and the sealing disk is attached to the lower surface of the filtrate net below. At least two electric push rods are fixedly provided at the bottom of the tank body, and the output rods of the electric push rods are connected to the sealing disk. A vortex groove is formed on the upper surface of the sealing disk, and a vortex hose is coiled in the vortex groove, and a seepage hole is opened on the vortex hose, and the free pipe mouth of the guide hose is connected to the drainage conduit arranged on the tank body through a connecting hose, and the top of the tank body is connected to a drainage elbow, and the bottom pipe mouth of the drainage elbow is located on the surface of the filtrate net above.

5. The device for purifying and preparing bovine spleen peptide for enhancing immunity according to claim 2, characterized in that: A shearing assembly is provided on the fan-shaped plate, which includes a square mesh plate. A through square hole is penetrated on the fan-shaped plate, in which a square mesh plate is sealed and inserted, and a recessed square hole is provided at the outer opening of the through square hole. Square strips are fixed at the upper and lower ends of the square mesh plate, and the square strips are connected to the bottom of the recessed square hole through a spring member, and the size of the square strips is the same as that of the recessed square hole.

6. The device for purifying and preparing bovine spleen peptide for enhancing immunity according to claim 2, characterized in that: The bottom end of the shaft tube extends out of the tank body and is connected to the fixed servo motor through the gear assembly. The bottom end of the screw member extends out of the shaft tube and is connected to the fixed servo motor. The top end of the shaft tube is in a sealed state and is rotatably connected to the filtrate net above.

7. The device for purifying and preparing bovine spleen peptide for enhancing immunity according to claim 1, characterized in that: The side wall of the tank body is connected with a waste discharge pipe, and the waste discharge pipe is located on the upper surface of the filtrate net.

8. A method for purifying and preparing bovine spleen peptide for enhancing immunity, the method being applicable to the bovine spleen peptide purification and preparation device as claimed in any one of claims 1 to 7, characterized in that: The method comprises the following steps: S1: Raw material preparation: Select high-quality beef spleen as raw material, clean and chop it for pretreatment; S2: Enzymatic hydrolysis: Mix the pretreated bovine spleen with water or buffer, put it into the tank through the feeding tube, add an appropriate amount of protease, and perform enzymatic hydrolysis under appropriate temperature and pH conditions to hydrolyze the protein into peptides; S3: Filtration: Remove impurities in the mixed slurry through the cooperation of the sealing pressure plate and the filtrate net formed in the tank body; S4: Separation and purification: Use ultrafiltration or membrane separation technology to separate the peptides in the enzymatic hydrolysate; use chromatography technology to further separate and purify the peptides; S5: Concentration and drying: Concentrate the purified peptide solution to remove excess solvent; freeze-dry or spray-dry the concentrate to form a powdered bovine spleen peptide product.

Images