A portable detection device and method for highly selective trace scandium ions

Abstract

This invention discloses a portable detection device and method for highly selective trace scandium ions, belonging to the field of rare earth metal ion detection technology. The detection device of this invention has a simple structure, relies on fluorescence sensing for detection, and is convenient, accurate, and low-cost. The detection method of this invention does not require the additional synthesis of organic ligands, making it environmentally friendly; by adjusting the fluorescence intensity of the complex under unsaturated complexation conditions, the fluorescence intensity of the complex increases with the amount of scandium ions detected. 3+ The design utilizes the principle that increases with increasing concentration to achieve [the desired effect] on Sc. 3+ It provides real-time on-site analysis and has the advantages of high sensitivity and high selectivity.

Description

Technical Field

[0001] This invention belongs to the field of rare earth metal ion detection technology, specifically relating to a portable detection device and method for highly selective trace scandium ions. Background Technology

[0002] Scandium (Sc), yttrium (Y), and the 15 lanthanide metals of the sixth period are collectively known as rare earth metals due to their similar physicochemical properties. Rare earth elements are scarce, non-renewable, and widely used, making them strategic national resources. China is a major global supplier of rare earth elements. Scandium metal and its compounds, in particular, possess unique electrical, optical, and magnetic properties, and are widely used in metallurgy, superconductors, supermagnetism, laser crystals, nuclear industry, aerospace, catalysis, and emerging fields such as solid-state fuel cells. As an important national strategic resource, the exploration and development of scandium ore plays a driving role in my country's industrial development. However, due to the large-scale separation, purification, and processing of scandium ore, scandium from industrial waste and emissions is released into the environment, which can have adverse effects on ecosystems and human health, especially some undiscovered potential hazards. Therefore, the selective determination of trace scandium ions in the environment is of great significance. Although classified as a rare earth element, scandium is not scarce in total on Earth; it exists in a highly dispersed form in various ores, which is one of the reasons why scandium is difficult to detect.

[0003] Scandium is located in the third subgroup of the third period. After losing electrons, it has only one stable trivalent ion state (Sc). 3+ Currently used for detecting Sc 3+ Traditional instrumental methods include atomic absorption spectrometry, inductively coupled plasma mass spectrometry, inductively coupled plasma emission spectrometry, and neutron activation analysis. These analytical strategies all have certain limitations, such as requiring complex and expensive equipment, specialized technicians, time-consuming sample pretreatment, and cumbersome operations. To address these issues, researchers have also developed some relatively convenient methods, such as spectrophotometry, fluorescence methods, and electrochemical methods. Among these methods, fluorescence methods utilize the synthesis or selection of fluorescent chelating ligands with specific properties to interact with Sc... 3+ The action causes a change in fluorescence intensity, and fluorescence methods are highly sensitive, especially turn-on fluorescence, which has a high signal-to-noise ratio and low background signal, and can identify trace amounts of Sc. 3+ However, most chelating ligands do not have specificity for complexing with scandium, and face challenges such as low selectivity and susceptibility to interference from other metal ions. Therefore, there is an urgent need to develop a highly selective and sensitive on-type fluorescence strategy for the detection of scandium ions.

[0004] With the rapid development of technology, smartphones have become detection components for various analytical methods, possessing capabilities such as data storage, analysis, and sharing, playing a crucial role in portable real-time detection. To date, no Sc integrated with smartphones has been developed. 3+ Therefore, it is necessary to develop a rapid and reliable portable fluorescence sensing platform and detection method for on-site measurement of Sc. 3+ On-site testing. Summary of the Invention

[0005] To address the aforementioned shortcomings of existing technologies, this invention provides a portable detection device and method for highly selective trace scandium ions, capable of detecting Scandium ions. 3+ It offers simple, fast, and real-time on-site analysis, with a detection linearity range of 0.04μM to 2.25μM and a detection limit of 0.02μM.

[0006] In a first aspect, the present invention provides a portable detection device for highly selective trace scandium ions, comprising a base and a housing movably disposed on the base;

[0007] The base is equipped with a light source and a sample cell, and the light source is a 470nm LED lamp;

[0008] A 500nm filter and a camera port are provided above the sample cell, and the camera port is located at the top of the outer shell;

[0009] The side wall of the housing is equipped with a battery and a battery switch, which serve as the power source for the light source.

[0010] Furthermore, the base and outer shell are made of black opaque resin and are manufactured using 3D printing.

[0011] Furthermore, the sample cell is cylindrical with a diameter of 10 mm and a height of 8 mm; the sample cell holds a sample solution volume of 250 μL.

[0012] Furthermore, the parameters of the portable detection device for highly selective trace scandium ions include: the overall length of the device is 75mm, the width is 55mm, and the height is 95mm; the excitation wavelength of the LED lamp is 470nm; the filter is 500nm; the quartz sample cell has a height of 8mm and a diameter of 10mm; and the power supply is two 1.5V dry batteries.

[0013] Secondly, the present invention provides a method for using the portable detection device for highly selective trace scandium ions as described in any of the first aspects, namely, a portable detection method for highly selective trace scandium ions, comprising the following steps:

[0014] Prepare scandium chloride mother liquor, scandium chloride solutions of varying concentrations, hydrochloric acid solution, and calcein solution;

[0015] Prepare samples and measure the fluorescence intensity of each sample sequentially using a fluorescence meter, and plot a standard curve;

[0016] Assemble the portable detection device for highly selective trace scandium ions, turn on the light source, take a picture of the solution after the sample reaction using a mobile phone camera, read the data from the picture, and obtain the linear regression equation;

[0017] The portable detection device for highly selective trace scandium ions was used to measure the water sample to be tested, and then a standard solution was added for recovery measurement. The spiked recovery rate of the method and the device was obtained to verify the accuracy.

[0018] Using the portable high-selectivity trace scandium ion detection device, the ore sample to be tested is soaked in hydrochloric acid solution, filtered, and the filtrate is reacted. The portable high-selectivity trace scandium ion detection device is then used to take pictures and detect the scandium ion concentration in the ore sample to be tested.

[0019] Furthermore, the concentration of the scandium chloride mother liquor is 100 mmol / L, the gradient concentration scandium chloride solution is prepared by diluting the scandium chloride mother liquor to 2-500 μmol / L, the pH value of the hydrochloric acid solution is 2.0, and the concentration of the calcein solution is 100 μmol / L.

[0020] Furthermore, the calcein solution is prepared by the following method:

[0021] Prepare a 10 mmol / L calcein solution and dilute it with the hydrochloric acid solution to obtain a 100 μmol / L calcein solution.

[0022] Further, the sample preparation includes: preparing 9 centrifuge tubes, adding 950 μL of hydrochloric acid solution and 50 μL of calcein solution to the first centrifuge tube as a blank control group, and adding 940 μL of hydrochloric acid solution, 50 μL of calcein solution and scandium chloride solution of varying concentrations to the remaining 8 centrifuge tubes.

[0023] Furthermore, the water sample determination includes the following steps: after filtering the water sample to be tested, a masking agent is added and then filtered again to obtain the filtrate. After adjusting the pH value to 2.0, calcein solution is added to react. The reacted solution is then placed into the sample cell, photographed, and detected to obtain the scandium ion concentration in the water sample to be tested.

[0024] Further, after the water sample is measured, a standard solution is added for recovery and determination, including the following steps: 200 μmol / L scandium standard solution is mixed with hydrochloric acid solution and calcein solution at a volume ratio of 10:940:50, and then photographed and detected using the aforementioned portable detection device for highly selective trace scandium ions.

[0025] Furthermore, the detection of scandium ion concentration in the ore sample to be tested includes the following steps: the ore sample to be tested is crushed, soaked in hydrochloric acid solution and sonicated, filtered to remove insoluble ore solids, a masking agent is added and filtered again, then the pH is adjusted to 2.0, calcein solution is added to react, the solution after reaction is aspirated and placed in the sample cell, photographed and detected to obtain the scandium ion concentration in the ore sample to be tested.

[0026] Compared with the prior art, the technical solutions provided in this application have at least the following advantages:

[0027] 1. This invention discloses a portable detection device for highly selective trace scandium ions. It has a simple structure, is easy to use, and its combination with a smartphone sensing platform meets the requirements for timely on-site detection. Moreover, the detection cost is low.

[0028] 2. This invention discloses a portable method for the highly selective detection of trace scandium ions. The principle is based on the fact that, under simple acidic solution matrix conditions, the fluorescent chelating ligand indicator calcein acts as a Lewis base and reacts with a Lewis acid (Sc... 3+ It can perform specific complexation with a complexation ratio of 2:1, Sc 3+ Upon complexation with calcein, the fluorescence of calcein, which was previously closed, is activated and affects Sc. 3+ It has unique selectivity. Therefore, this invention is based on Sc. 3+ The strong Lewis acid properties of Sc have led to the establishment of a portable, open-type fluorescent sensing device and detection method for the first time. 3+ By complexing with the chelating ligand calcein, green fluorescence (optimal emission 500 nm) was achieved at pH 2.0 without the need for additional reagents and materials, and the fluorescence intensity increased with increasing scandium ion concentration. The calcein fluorescent probe also demonstrated its effectiveness against Scandium ions. 3+ The detection method exhibits high selectivity and high sensitivity, enabling the specific detection of trace amounts of Sc from 17 rare earth elements and common metal ions. 3+ Simultaneously, a 3D-printed portable fluorescence sensing device (a portable detection device for highly selective trace scandium ions) was designed to be integrated with a smartphone, converting fluorescence intensity into a color channel (G value), which is simple, accurate, and efficient to operate.

[0029] 3. The portable method for highly selective trace scandium ion detection disclosed in this invention needs to be carried out under HCl-H2O conditions at pH 2.0, where the background fluorescence of calcein is low. The addition of trace amounts of Sc... 3+ The fluorescence is activated after the initial reaction, resulting in high detection sensitivity. Furthermore, the detection can be achieved using the existing chelating ligand calcein, eliminating the need for synthesizing additional organic ligands, making it convenient, rapid, and environmentally friendly. Under these conditions, the complexation of calcein with other metal ions is far less than with Sc.3+ It exhibits strong complexation and high selectivity, unaffected by other rare earth metals, and can be used for portable fluorescence sensing detection of trace scandium ions. Attached Figure Description

[0030] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this application and, together with the description, serve to explain the principles of this application.

[0031] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0032] Figure 1 A schematic diagram illustrating the principle of the portable detection of highly selective trace scandium ions based on this invention;

[0033] Figure 2 A schematic diagram illustrating the combination of a smartphone and a portable detection device for highly selective trace scandium ions;

[0034] Figure 3 Standard curve determined for a portable detection device for highly selective trace scandium ions;

[0035] Figure 4 This is a schematic diagram of the structure of a portable detection device for highly selective trace scandium ions provided in Example 1;

[0036] Figure 5 This is a flowchart of the scandium ion analysis in the ore sample in Example 2;

[0037] Figure 6 This is the standard curve measured using a fluorescence spectrometer in Example 2;

[0038] Figure 7 This is a schematic diagram illustrating the use of a portable detection device for highly selective trace scandium ions to continuously measure 11 parallel samples 11 times. Detailed Implementation

[0039] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0040] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this application can be purchased from the market or prepared by existing methods.

[0041] The principles and features of the present invention are described below with reference to embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified, specific conditions or manufacturer-recommended conditions should be followed in the embodiments. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0042] In the following examples, hydrochloric acid was purchased from Chengdu Kelon Chemicals Co., Ltd. (Chengdu, China), calcein was purchased from Yuanye Biotechnology Co., Ltd. (Shanghai, China), and scandium chloride hexahydrate was purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China).

[0043] Example 1

[0044] like Figure 2 and Figure 4 As shown in the figure, this embodiment provides a portable detection device for highly selective trace scandium ions, including a movably connected base and a housing. The base has a sample cell, which is cylindrical and used to hold the sample to be tested. The sample cell is made of quartz, has a diameter of 10 mm, a height of 8 mm, and a solution volume of 250 μL.

[0045] The entire portable testing device has a box-shaped structure, measuring 75mm in length, 55mm in width, and 105mm in height, and is made of black opaque resin using 3D printing.

[0046] A battery pack and a light source are located on the inner wall of the casing. The battery pack is controlled by a battery switch on the outer wall of the casing, and the battery provides power to the light source. The light source is a 470nm LED, 3V and 3W.

[0047] At the top of the sample cell, there is a camera port on the top of the outer shell, and a 500nm filter is placed below the camera port.

[0048] To facilitate the use of a portable detection device for highly selective trace scandium ions, using a Huawei P30 smartphone as an example, the phone's camera is set to professional shooting mode, ISO: 100, s: 1 / 60s.

[0049] Example 2

[0050] This embodiment provides a portable method for the detection of trace scandium ions with high selectivity. The samples to be tested are water samples and mineral samples. The specific steps are as follows:

[0051] (1) Preparation of scandium chloride mother liquor

[0052] Accurately weigh 0.02595 g of scandium chloride hexahydrate into a 2 mL centrifuge tube, add 1 mL of ultrapure water to dissolve it, and obtain a scandium chloride solution with a concentration of 100 mmol / L.

[0053] (2) Prepare a series of gradient scandium chloride solutions

[0054] The 100 mmol / L scandium chloride solution was serially diluted to the required concentration gradient (2 μmol / L to 500 μmol / L) for later use.

[0055] The concentrations included were 0.04 μmol / L, 0.1 μmol / L, 0.2 μmol / L, 0.5 μmol / L, 1.0 μmol / L, 1.5 μmol / L, 2.0 μmol / L, and 2.25 μmol / L.

[0056] (3) Prepare a hydrochloric acid solution with pH = 2.0.

[0057] Prepare concentrated hydrochloric acid diluted 100 times and 1000 times, and 150 mL of ultrapure water. Use a dropper to gradually add the diluted hydrochloric acid to the ultrapure water in small amounts, stir well, and read the pH value of the solution with a pH meter. When the pH of the solution reaches 2.0, collect it for later use.

[0058] (4) Prepare calcium chlorophyll solution

[0059] Accurately weigh 0.00623 g of calcein into a 2 mL centrifuge tube, add 1 mL of dimethyl sulfoxide to dissolve it, and obtain a calcein solution with a concentration of 10 mmol / L. Take 10 μL of the 10 mmol / L calcein solution and dilute it to 1000 μL with HCl-H2O at pH=2.0 to obtain a 100 μmol / L calcein solution for later use.

[0060] (5) Sample preparation

[0061] Prepare nine centrifuge tubes. Add 950 μL of HCl-H₂O and 50 μL of calcein solution to the first tube. Add 940 μL of HCl-H₂O, followed by 50 μL of calcein solution, to the remaining eight 2 mL centrifuge tubes. Finally, add 10 μL of a series of scandium chloride solutions with varying concentrations to achieve final scandium chloride concentrations of 0.04 μmol / L, 0.1 μmol / L, 0.2 μmol / L, 0.5 μmol / L, 1.0 μmol / L, 1.5 μmol / L, 2.0 μmol / L, and 2.25 μmol / L, for a total volume of 1 mL. Perform three parallel tests on each sample.

[0062] (6) Determine the standard curve using a fluorescence spectrophotometer.

[0063] The fluorescence intensity of each solution in step (5) was measured sequentially using a fluorescence spectrophotometer. The total solution volume was 1 mL. The parameters were set as follows: excitation wavelength 470 nm, slit width 1 nm, and emission intensity at 500 nm. The fluorescence intensities of each group are shown in Table 1. A standard curve was plotted using Origin, with the ordinate being F / F0. F represents the fluorescence intensity in the presence of different concentrations of scandium ions, and F0 represents the fluorescence intensity when the scandium ion concentration is 0. The standard curve is plotted as follows. Figure 6 As shown.

[0064] Table 1

[0065]

[0066]

[0067] (7) Use the developed portable device to plot the standard curve.

[0068] The developed portable fluorescence sensing device, combined with colorimetric analysis software, was used to sequentially read the G values ​​of each solution in step (5). The excitation wavelength of the LED beads was 470 nm, and the wavelength of the filter was 500 nm. 250 μL was added to the quartz sample cell. The parameters of the mobile phone camera were fixed in professional mode, and three parallel samples were measured. The Colorpicker software was opened for colorimetric analysis, and the G channel value in RGB was obtained. The G values ​​of each group are shown in Table 2. The scandium chloride concentration corresponding to ΔG(G-G0, where G is the value containing different concentrations of scandium chloride, and G0 is the blank value without scandium chloride) was imported into Origin software for graph analysis. The linear regression equation was obtained, as shown in Table 3. The standard curve was plotted as follows. Figure 3 As shown.

[0069] Table 2

[0070]

[0071] Table 3

[0072] target Linear regression equation linear correlation coefficient Linear range (μmol / L) <![CDATA[Sc 3+ ]]> y = 16.33x + 1.66 0.99 0.02～2.25

[0073] (8) Stability test

[0074] At pH 2.0, 11 parallel samples containing 5 μmol / L calcein and 2 μmol / L scandium chloride were measured 11 times consecutively using a portable fluorescence sensor. The results are as follows: Figure 7 As shown, the specific operation is the same as step (7).

[0075] (9) Water sample testing

[0076] The collected water samples were filtered through a 0.22μm microporous membrane to remove impurities, sealed with sealing glue, and stored in a refrigerator at 0-4℃ for later use.

[0077] Water sample treatment process as follows Figure 5 As shown (steps 1 and 2 are not required), add 1mM of S. 2- (Masking agent), then filter with a 0.22μm filter, collect the filtrate, and adjust the pH to 2.0 using a portable pH meter. Take 950μL of the supernatant and add 50μL of 100μmol / L calcein, mix well, and pipette 250μL of the prepared sample into the quartz sample cell. Cover the device to ensure no interference from external light sources, place the mobile phone in the portable device slot, set the camera to professional mode, turn on the excitation light power supply, take a picture, measure 3 parallel samples, and open the Color picker software for color analysis.

[0078] (10) Comparison of national standards

[0079] The accuracy of the developed portable fluorescence sensing device was verified using inductively coupled plasma mass spectrometry (ICP-MS / MS). Instrument parameters were set as follows: power, 1550W; auxiliary gas flow rate, 0.1L·min. -1 Atomizing gas flow rate, 0.1 L·min -1 Detection mode: no gas; three parallel measurements.

[0080] (11) Spike Recovery Experiment

[0081] A standard solution was added for recovery determination (the purpose of which is to determine the recovery rate and compare the accuracy of the method if scandium is not detected in the actual sample). 940 μL of hydrochloric acid solution and 50 μL of calcein were added to three 2 mL centrifuge tubes, followed by 10 μL of 200 μmol / L scandium standard solution to a total volume of 1 mL. After thorough mixing, 250 μL was added to a quartz sample cell, and three parallel samples were analyzed. Colorimetric analysis was performed using Color Picker software, and the results are shown in Table 4. Neither method detected scandium in the lake. The spiked recovery experiment showed a recovery rate of 90-114%, indicating that it can be used for the detection of scandium in real water samples.

[0082] Table 4

[0083]

[0084] Note: a It represents the average value ± the standard deviation of three measurements, and ND indicates that it was not measured.

[0085] (12) Detection of ore samples

[0086] like Figure 5As shown, the ore sample was soaked in 50 mL of pH 2.0 hydrochloric acid solution and sonicated for 80 min. The remaining steps were the same as in (9). The analytical results are shown in Table 5. Neither method detected scandium content in quartzite, kaolinite, or manganese ore. Scandium was detected in bauxite. The detection value of this method was 0.71 μmol / L, and the detection value of the national standard method was 0.67 μmol / L. F = 4.24 <F 0.05,5,2 =19.3, indicating no significant difference in precision between the two sets of data, t=1.91 <t 0.05,2 =4.30, there was no significant difference in the results of the two methods, and the spiked recovery rate was between 81% and 117%, indicating that the method can be used for the detection of rare earth minerals in real ore samples. The comparison of the national standard method is consistent with step (10).

[0087] Table 5

[0088]

[0089] a Mean ± standard deviation of three measurements; ND indicates not measured.

[0090] Figure 5 In the diagram, 1 represents the step of crushing the ore sample, 2 represents the step of soaking in hydrochloric acid, 3 represents the step of adding a masking agent, 4 represents the step of filtering and collecting the filtrate, 5 represents the step of adjusting the pH to 2.0, 6 represents the step of taking a picture with a mobile phone after adding calcein, and 7 represents the process of software analysis.

[0091] In summary, this invention enables the use of industrially produced fluorescent chelating ligand indicator Calcein as a Lewis base to react with the strong Lewis acid Sc in a simple acidic solution matrix (i.e., hydrochloric acid solution with pH 2.0). 3+ Specific complexation, forming a complexation ratio of 2:1 (calcein:Sc) 3+ ) complexes, Sc 3+ Upon complexation with calcein, the fluorescence of calcein, which was previously closed, is activated and affects Sc. 3+ It has unique selectivity, the principle of which is as follows: Figure 1 As shown.

[0092] Under the condition of unsaturated complexation ratio, the fluorescence intensity of the complex increases with Sc 3+ The concentration of scandium increases with increasing fluorescence intensity. A portable fluorescence sensing device (i.e., a highly selective portable detection device for trace scandium ions) was designed using a smartphone detection device in Example 1 of this invention. It is simple, accurate, with a detection linear range of 0.04 μM to 2.25 μM and a detection limit of 0.02 μM, meeting the requirements of point-of-care testing (POCT). The proposed method does not require the additional synthesis of organic ligands, is low-cost, environmentally friendly, and can achieve the detection of Sc... 3+Simple, fast, and allows for real-time on-site analysis.

[0093] The above description is merely a specific embodiment of this application, enabling those skilled in the art to understand or implement this application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of this application. Therefore, this application is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A portable method for the detection of highly selective trace scandium ions, based on a portable detection device for highly selective trace scandium ions, characterized in that, Includes the following steps: Prepare scandium chloride mother liquor, scandium chloride solutions of varying concentrations, hydrochloric acid solution, and calcein solution; Assemble the portable detection device for highly selective trace scandium ions, turn on the light source, take a picture of the solution after the sample reaction using a mobile phone camera, read the data from the picture, and obtain the linear regression equation; Add a masking agent to the water sample or ore sample to be tested, filter, take the supernatant and adjust the pH value to 2.0, then add the calcein solution to react, and use the portable detection device for highly selective trace scandium ions to photograph and detect the solution after the reaction to obtain the scandium ion concentration in the water sample or ore sample to be tested. The portable detection device for highly selective trace scandium ions includes a base and a housing movably disposed on the base; The base is equipped with a light source and a sample cell, and the light source is a 470nm LED lamp; A 500nm filter and a camera port are provided above the sample cell, and the camera port is located at the top of the outer shell; The outer casing is equipped with a battery and a battery switch, which serve as the power source for the light source; the base and the outer casing are made of black opaque resin and are manufactured using 3D printing. The sample cell is cylindrical with a diameter of 10 mm and a height of 8 mm; the sample cell holds a sample solution volume of 250 μL.

2. The portable detection method for highly selective trace scandium ions according to claim 1, characterized in that, The concentration of the scandium chloride mother liquor is 100 mmol / L, the gradient concentration scandium chloride solution is prepared by diluting the scandium chloride mother liquor to 2~500 μmol / L, the pH value of the hydrochloric acid solution is 2.0, and the concentration of the calcein solution is 100 μmol / L.

3. The portable detection method for highly selective trace scandium ions according to claim 1, characterized in that, The calcein solution was prepared by the following method: Prepare a 10 mmol / L calcein solution and dilute it with the hydrochloric acid solution at pH 2.0 to obtain a 100 μmol / L calcein solution.

4. The portable detection method for highly selective trace scandium ions according to claim 1, characterized in that, The sample reaction process included: preparing 9 centrifuge tubes, adding 950 μL of hydrochloric acid solution and 50 μL of calcein solution to the first centrifuge tube, and adding 940 μL of hydrochloric acid solution, 50 μL of calcein solution and 10 μL of scandium chloride solution of varying concentrations to the remaining 8 centrifuge tubes.

5. The portable detection method for highly selective trace scandium ions according to claim 1, characterized in that, Before obtaining the linear regression equation using a portable fluorescence sensor, the fluorescence intensity of the solution after the sample reaction is measured using a fluorescence meter, and a standard curve of the standard sample is obtained to indicate feasibility.

6. The portable detection method for highly selective trace scandium ions according to claim 1, characterized in that, The detection of scandium ion concentration in the water sample to be tested includes the following steps: After routine pretreatment and filtration of the water sample to be tested, a masking agent is added and then filtered again to obtain the filtrate. The pH value is adjusted to 2.0, and then calcein solution is added to react. The solution after reaction is aspirated and placed into the sample cell, photographed and detected to obtain the scandium ion concentration in the water sample to be tested.

7. The portable method for highly selective trace scandium ions according to claim 1, characterized in that, The detection of scandium ion concentration in the ore sample to be tested includes the following steps: the ore sample to be tested is crushed, soaked in hydrochloric acid solution and sonicated, filtered and the filtrate is collected, a masking agent is added, then filtered again, the pH is adjusted to 2.0, calcein solution is added to react, the solution after reaction is aspirated and placed in the sample cell, photographed and detected, and the scandium ion concentration in the ore sample to be tested is obtained.

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