Device and method for detecting non-specific syphilis antibody

Abstract

The invention provides a syphilis non-specific antibody detection device and method, and belongs to the technical field of medical instruments. The problem of misreading of a critical sample due to manual operation and reading in the existing syphilis non-specific antibody detection is solved. The syphilis non-specific antibody detection device comprises a transparent sample cup, an optical detection system and a stirring structure used for uniformly mixing a sample and a reagent, the optical detection system comprises a transmitting end arranged on one side of the sample cup and a receiving end arranged on the other side of the sample cup, and the transmitting end is used for transmitting light to the receiving end; the receiving end is used for receiving and processing the light penetrating through the sample cup. A detection process curve is drawn through an optical detection system, the reaction value of the whole stage can be tested, and a positive detection result value can be accurately given.

Description

Technical Field

[0001] The invention belongs to the technical field of medical devices and relates to a device and method for detecting non-specific syphilis antibodies. Background Art

[0002] At present, the detection of non-specific antibodies to syphilis basically adopts the two test methods of TRUST and RPR. The operator processes the sample on the test paper card and then puts the sample on the horizontal oscillator to run the test for 8 minutes. After the test is completed, the tester determines whether the result is negative or positive. Due to manual operation and reading, there may be misreading of critical samples, and there is no detailed quantitative result. The result is only negative and positive. The degree of positivity needs to be further diluted and tested. The test result is also the dilution ratio value, without the exact test value, and the result does not reflect the situation of the test process. Summary of the invention

[0003] The purpose of the present invention is to solve the above problems in the prior art and to provide a syphilis non-specific antibody detection device which can automatically read the detection results.

[0004] A method for detecting non-specific antibodies to syphilis is also proposed.

[0005] The purpose of the present invention can be achieved by the following technical solutions:

[0006] A detection device for non-specific syphilis antibodies includes a transparent sample cup, an optical detection system and a stirring structure for mixing samples and reagents. The optical detection system includes a transmitting end arranged on one side of the sample cup and a receiving end arranged on the other side of the sample cup. The transmitting end is used to emit light to the receiving end, and the receiving end is used to receive and process the light passing through the sample cup.

[0007] The transmitting end and the receiving end are arranged opposite to each other, the sample cup is located between the transmitting end and the receiving end, and the stirring structure is used to mix the sample and reagent in the sample cup. During the test, the reagent for detection is added. Affected by the color of the reagent, the detection curve will have a large change.

[0008] In the above-mentioned detection device for non-specific syphilis antibodies, the receiving end includes a photoelectric sensor, a filter circuit, an operational amplifier and an AD sampling chip. The signal output end of the photoelectric sensor is connected to the input end of the filter circuit, the output end of the filter circuit is connected to the input end of the operational amplifier, the output end of the operational amplifier is connected to the analog input end of the AD sampling chip, and the AD sampling chip converts the electrical signal into a digital signal.

[0009] Photoelectric sensors convert light into electrical signals, filtering circuits remove unnecessary frequency components and retain only useful electrical signals, operational amplifiers (op amps) amplify electrical signals for subsequent processing, and high-precision AD sampling chips convert electrical signals into digital signals and send them to microcontrollers, computers or other digital processing devices for further processing, such as data analysis, storage or display.

[0010] In the above-mentioned syphilis non-specific antibody detection device, the transmitting end is driven by a constant current source. The transmitting end is driven by a high-precision constant current source so that the light emitted by the transmitting end is constant.

[0011] In the above-mentioned syphilis non-specific antibody detection device, the stirring structure includes a motor arranged below the sample cup and a magnetic stirrer arranged in the sample cup. A magnet is installed on the rotating shaft of the motor. When the rotating shaft rotates, the magnet can drive the magnetic stirrer to rotate.

[0012] The method for detecting non-specific syphilis antibodies comprises the following steps:

[0013] Step 1: Put the sample and magnetic stirrer into the sample cup, and place the sample cup between the transmitting end and the receiving end;

[0014] Step 2: Start the motor and optical detection system;

[0015] Step 3: Add the detection reagent to the sample cup;

[0016] Step 4: The sample and the reagent are fully mixed under the action of a magnetic stirrer;

[0017] Step 5: The photoelectric sensor converts the received light into an electrical signal, the AD sampling chip converts the electrical signal into a digital signal, and a detection process curve is drawn by a computer.

[0018] When the sample is negative, the sample and the reagent do not react, the light does not change, and the test result is approximately a straight line; when the sample is positive, the sample and the reagent undergo an agglutination reaction, and the test process curve changes significantly.

[0019] In the above-mentioned method for detecting non-specific antibodies to syphilis, the detection process curve is a detection value-time curve, wherein the abscissa is the detection time and the ordinate is the detection value.

[0020] The detection process curve is divided into three stages. Stage 1 is the detection value when the sample is just added, stage 2 is the detection value when the reagent is added, and stage 3 is the reaction process after the reagent is added.

[0021] After the reaction is completed, the value at each time point can be obtained through the detection process curve, thereby obtaining the quantitative detection value, maximum value and other detection results of the sample.

[0022] In the above method for detecting non-specific antibodies to syphilis, the reagent is toluidine red dye (TRUST) or colloidal particles coated with BCG protein and phospholipid (RPR).

[0023] Compared with the prior art, the present invention has the following advantages:

[0024] The detection device has a reasonable structural design, can fully mix the sample and reagent, and draw a detection process curve through an optical detection system, can test the reaction value of the entire stage, and can accurately give a positive detection result value. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a structural schematic diagram of the detection device.

[0026] Figure 2 This is the connection diagram of the receiving end.

[0027] Figure 3 It is a curve diagram of the detection process.

[0028] In the figure, 1. sample cup; 2. transmitting end; 3. receiving end; 31. photoelectric sensor; 32. filter circuit; 33. operational amplifier; 34. AD sampling chip; 4. motor; 5. magnetic stirring bar; 6. magnet. DETAILED DESCRIPTION

[0029] The following are specific embodiments of the present invention and the accompanying drawings to further describe the technical solution of the present invention, but the present invention is not limited to these embodiments.

[0030] like Figure 1 The detection device for non-specific antibodies to syphilis shown comprises a transparent sample cup 1, an optical detection system and a stirring structure for mixing the sample and reagent in the sample cup 1. The optical detection system comprises a transmitting end 2 arranged on one side of the sample cup 1 and a receiving end 3 arranged on the other side of the sample cup 1. The transmitting end 2 and the receiving end 3 are arranged opposite to each other. The sample cup 1 is located between the transmitting end 2 and the receiving end 3. The transmitting end 2 is used to emit light to the receiving end 3, and the receiving end 3 is used to receive and process the light passing through the sample cup 1. During the test, a reagent for detection is added. Affected by the color of the reagent, the detection curve will have a large change.

[0031] like Figure 2 As shown, the receiving end 3 includes a photoelectric sensor 31, a filter circuit 32, an operational amplifier 33 and an AD sampling chip 34. The signal output end of the photoelectric sensor 31 is connected to the input end of the filter circuit 32, the output end of the filter circuit 32 is connected to the input end of the operational amplifier 33, the output end of the operational amplifier 33 is connected to the analog input end of the AD sampling chip 34, and the AD sampling chip 34 converts the electrical signal into a digital signal.

[0032] The photoelectric sensor 31 converts light into an electrical signal, the filter circuit 32 removes unnecessary frequency components and retains only useful electrical signals, the operational amplifier 33 (operational amplifier) ​​amplifies the electrical signal for subsequent processing, and the high-precision AD sampling chip 34 converts the electrical signal into a digital signal and sends it to a microcontroller, computer or other digital processing device for further processing, such as data analysis, storage or display.

[0033] The transmitting end 2 is driven by a high-precision constant current source, so that the light emitted by the transmitting end 2 is constant. Specifically, the transmitting end 2 is a laser transmitter or an LED lamp.

[0034] like Figure 1 As shown, the stirring structure includes a motor 4 arranged below the sample cup 1 and a magnetic stirrer 5 arranged in the sample cup 1. The rotating shaft of the motor 4 is vertically upward and coaxially arranged with the sample cup 1. A magnet 6 is installed at the upper end of the rotating shaft of the motor 4. When the rotating shaft of the motor 4 rotates, the magnet 6 is driven to rotate. When the magnet 6 rotates, the magnetic stirrer 5 located in the sample cup 1 is driven to rotate through magnetic energy, thereby mixing the sample and the reagent.

[0035] The method for detecting non-specific syphilis antibodies comprises the following steps:

[0036] Step 1: Put the sample and the magnetic stirrer 5 into the sample cup 1, and place the sample cup 1 between the transmitting end 2 and the receiving end 3;

[0037] Step 2: start the motor 4 and the optical detection system;

[0038] Step 3: Add the detection reagent to the sample cup 1;

[0039] Step 4: The sample and the reagent are fully mixed under the action of the magnetic stirrer 5;

[0040] Step 5: The photoelectric sensor 31 converts the received light into an electrical signal, the AD sampling chip 34 converts the electrical signal into a digital signal, and a detection process curve is drawn by a computer.

[0041] The test process curve is the test value-time curve, with the horizontal axis being the test time and the vertical axis being the test value. When the sample is negative, the sample and the reagent do not react, the light does not change, and the test result is approximately a straight line. When the sample is positive, the sample and the reagent undergo agglutination reaction, and the test process curve will change significantly.

[0042] The detection process curve is divided into three stages: stage 1 is the detection value when the sample is just added, stage 2 is the detection value when the reagent is added, and stage 3 is the reaction process after the reagent is added. After the reaction is completed, the detection process curve can be used to obtain the value at each time point, thereby obtaining the quantitative detection value, maximum value and other detection results of the sample.

[0043] In this embodiment, the reagent is toluidine red dye (TRUST) or colloidal particles coated with BCG protein and phospholipid (RPR).

[0044] The specific embodiments described herein are merely examples of the spirit of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in similar ways, but they will not deviate from the spirit of the present invention or exceed the scope defined by the appended claims.

Claims

1. A device for detecting non-specific antibodies to syphilis, characterized in that: The invention comprises a transparent sample cup (1), an optical detection system and a stirring structure for mixing a sample and a reagent. The optical detection system comprises a transmitting end (2) arranged on one side of the sample cup (1) and a receiving end (3) arranged on the other side of the sample cup (1). The transmitting end (2) is used to emit light to the receiving end (3), and the receiving end (3) is used to receive and process the light transmitted through the sample cup (1).

2. The detection device for non-specific syphilis antibodies according to claim 1, characterized in that: The receiving end (3) comprises a photoelectric sensor (31), a filter circuit (32), an operational amplifier (33) and an AD sampling chip (34); the signal output end of the photoelectric sensor (31) is connected to the input end of the filter circuit (32); the output end of the filter circuit (32) is connected to the input end of the operational amplifier (33); the output end of the operational amplifier (33) is connected to the analog input end of the AD sampling chip (34); and the AD sampling chip (34) converts the electrical signal into a digital signal.

3. The detection device for non-specific syphilis antibodies according to claim 1, characterized in that: The transmitting end (2) is driven by a constant current source.

4. The detection device for non-specific syphilis antibodies according to claim 1, characterized in that: The stirring structure comprises a motor (4) arranged below the sample cup (1) and a magnetic stirring bar (5) arranged in the sample cup (1); a magnet (6) is mounted on the rotating shaft of the motor (4); when the rotating shaft rotates, the magnet (6) can drive the magnetic stirring bar (5) to rotate.

5. A method for detecting non-specific antibodies to syphilis, characterized in that: The detection device according to any one of claims 1 to 4 comprises the following steps: Step 1: Place the sample and the magnetic stirrer (5) into a sample cup (1), and place the sample cup (1) between the transmitting end (2) and the receiving end (3); Step 2: starting the motor (4) and the optical detection system; Step 3: Adding a detection reagent into the sample cup (1); Step 4: The sample and the reagent are fully mixed under the action of a magnetic stirrer (5); Step 5: The photoelectric sensor (31) converts the received light into an electrical signal, the AD sampling chip (34) converts the electrical signal into a digital signal, and a detection process curve is drawn by a computer.

6. The method for detecting non-specific syphilis antibodies according to claim 5, characterized in that: The detection process curve is a detection value-time curve, where the horizontal axis is the detection time and the vertical axis is the detection value.

7. The method for detecting non-specific syphilis antibodies according to claim 5, characterized in that: The reagent is toluidine red dye or colloidal particles coated with BCG protein and phospholipid.

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