Steady-state phospholipid type DHA microcapsule as well as preparation method and application thereof

Abstract

The invention belongs to the field of polyunsaturated fatty acid oil preparations, and discloses a steady-state phospholipid type DHA microcapsule as well as a preparation method and application thereof. The preparation method comprises the following steps: weighing resistant dextrin and soybean protein isolate as embedding wall materials according to a certain ratio, uniformly stirring, adding water, stirring and dissolving to form a wall material solution, adding polysaccharide into the wall material solution to obtain a solution A, adding core material grease into the solution A according to a certain ratio, stirring and dispersing, performing high-pressure homogenization to prepare an emulsion, drying, and sieving to obtain the high-temperature-resistant oil-in-oil-in-oil-in-oil-in-oil-in-oil-in-oil-in-oil-in-oil-in-oil-in-oil-in-oil-in-oil. The phospholipid type DHA microcapsule powder is subjected to subcritical treatment, and the steady-state phospholipid type DHA microcapsule powder is obtained. Polysaccharide substances with the molecular weight smaller than 10,000 Daltons are added into an embedding material, stability of polyunsaturated fatty acid grease can be provided, and the prepared phospholipid type DHA microcapsule is free of surface oil, complete in structure, good in taste masking effect, free of fishy smell or similar to fishy smell after being placed for 3 months and excellent in storage stability.

Description

Technical Field

[0001] The invention belongs to the field of polyunsaturated fatty acid oil preparations, and in particular relates to a stabilized phospholipid-type DHA microcapsule and a preparation method and application thereof. Background Art

[0002] Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), α-linolenic acid (ALA), etc. are a class of physiologically active omega-3 polyunsaturated fatty acids. Among them, DHA helps improve brain development and cognitive function, can regulate the body's lipid metabolism and immune function, and has multiple functions such as preventing and treating cardiovascular diseases. At present, the raw materials of DHA products sold on the market are mainly derived from fish oil, algae oil and krill oil. The raw material types are mainly ethyl ester DHA, triglyceride DHA and phospholipid DHA. Phospholipid DHA is mainly derived from krill oil. Existing studies have shown that compared with ethyl ester DHA and triglyceride DHA, phospholipid DHA is easier to pass through the blood-brain barrier and has higher bioavailability, so it has attracted the attention of consumers.

[0003] At present, the DHA products sold on the market are mainly in the form of capsules and drops, and the product forms are relatively simple. This is mainly because DHA derived from fish oil has a certain fishy smell. In addition, polyunsaturated fatty acid oils are easily oxidized to produce unpleasant odors. The use of encapsulation microencapsulation technology to protect polyunsaturated fatty acid oils can mask the taste and delay oxidation. However, polyunsaturated fatty acid oil encapsulation microencapsulation also has certain disadvantages:

[0004] 1) Low oil loading and DHA content. Through the retrieval of existing literature and the collection of existing products, the oil loading of oil microcapsules is mainly concentrated in 15-50%, and the DHA+EPA content in fish oil and krill oil phospholipids does not exceed 30%. This leads to a lower DHA content in the embedded microencapsulated powder, which cannot meet the high concentration requirements of downstream products.

[0005] 2) Poor stability Microencapsulation is the process of mixing the wall material with DHA oil to make an emulsion, and then using spray drying, freeze drying, fluidized bed drying and other methods to encapsulate the DHA oil droplets into tiny particles. Polyunsaturated fatty acid oil embedding microencapsulation is a complex and non-directional process, and its final quality is affected by various complex factors such as components (embedded wall materials, core oils), processes, storage conditions, etc. For example, unreasonable selection of microencapsulation component materials, wrong ratios leading to low microencapsulation efficiency, insufficient density of the outer wall structure leading to high surface oil content, incomplete taste masking, and easy oxidation to produce unpleasant odors, and color changes, resulting in inability to be used downstream.

[0006] 3) Lack of elimination process. Microencapsulation of polyunsaturated fatty acid oils is a complex and non-directional process of encapsulating polyunsaturated fatty acid oils into irregular spheres with a certain volume. In the microencapsulated powder of the final product of polyunsaturated fatty acid oils, there is adhesion of surface oil, insufficient outer wall structure and hollow shell structure. There is currently no process to eliminate these "failed" microencapsulated powders, resulting in poor thermal stability and mechanical properties of the microencapsulated powders. Further, they are easily oxidized during storage, producing unpleasant odors and harmful substances, which ultimately affects the downstream application prospects. Summary of the invention

[0007] In order to overcome the shortcomings and deficiencies of the prior art, the primary purpose of the present invention is to provide a method for preparing stabilized phospholipid-type DHA microcapsules.

[0008] Another object of the present invention is to provide a stabilized phospholipid DHA microcapsule.

[0009] Another object of the present invention is to provide an application of the stabilized phospholipid DHA microcapsules.

[0010] The present invention unexpectedly discovered that polysaccharide substances, especially low molecular weight polysaccharide substances (less than 10,000 Daltons), can be used as embedding materials to improve the stability of polyunsaturated fatty acid oils during the preparation process of stabilized polyunsaturated fatty acid oil microcapsules. In addition, the oil adhering to the surface of the microcapsule powder can be washed away by subcritical treatment of the polyunsaturated fatty acid oil microcapsule powder. In the process of subcritical washing away the oil adhering to the surface of the microcapsule powder, the oil in the outer wall structure that is not dense enough and the shell structure that is hollow can also be washed away. Because the subcritical equipment is under pressure during operation, this part of "failed" microcapsule powder can be compressed to make its particle size smaller (relative to normal oil microcapsules), and then the microcapsule powder with the outer wall structure that is not dense enough and the shell structure that is hollow can be removed by sieving, so that the final microcapsule powder has a precise structure and no hollows, reaches a stable state, and can be stored for a long time.

[0011] The purpose of the present invention is achieved through the following technical solutions:

[0012] A method for preparing stabilized phospholipid DHA microcapsules comprises the following steps:

[0013] (1) Weighing resistant dextrin and soy protein isolate as embedding wall materials in proportion, stirring evenly, adding water and stirring to dissolve into a wall material solution;

[0014] (2) adding polysaccharide to the wall material solution, stirring and dispersing until the polysaccharide is completely dissolved, to obtain solution A;

[0015] (3) adding the core material oil to the solution A prepared in step (2) in a certain proportion, stirring and dispersing, and then high-pressure homogenizing to prepare an emulsion;

[0016] (4) drying and sieving the emulsion of step (3) to prepare phospholipid DHA microcapsule powder;

[0017] (5) The phospholipid DHA microcapsule powder prepared in step (4) is subjected to subcritical treatment and sieved to obtain stabilized phospholipid DHA microcapsule powder.

[0018] Preferably, the mass ratio of the resistant dextrin to the isolated soy protein is 1-3:1-3;

[0019] Preferably, the mass concentration of the wall material solution in step (1) is 5%-30%.

[0020] Preferably, the polysaccharide is one or more of plant polysaccharides or microbial polysaccharides, and the molecular weight of the polysaccharide is less than 10,000 Daltons, preferably less than 1,000 Daltons, and more preferably less than 500 Daltons.

[0021] Preferably, the polysaccharide is at least one of Lentinan, Poria cocos polysaccharide and Hericium erinaceus polysaccharide;

[0022] Preferably, the added amount of the polysaccharide is 5%-20% of the weight of the embedding wall material, preferably 10-15%.

[0023] Preferably, the mass ratio of the core material grease to the embedded wall material is 1-2:1, preferably 1.2-1.5:1.

[0024] Preferably, the core material oil is phospholipid-type DHA, which is derived from one or a mixture of soybean lecithin or krill oil lecithin, and is prepared by immobilized enzyme catalysis, wherein the DHA content is 40-70wt.%, preferably 60-70wt.%.

[0025] Preferably, the conditions of the high-pressure homogenization in step (3) are: the homogenization pressure is 20-50 MPa, preferably 30-40 MPa, the homogenization times are 2-7 times, preferably 3-5 times, and the homogenization temperature is 0-20° C., preferably 2-10° C.;

[0026] Preferably, the dispersion conditions in step (3) are 8000-150000 r / min for 5-20 min;

[0027] The drying in step (4) is spray drying or freeze drying, and the phospholipid DHA microcapsule powder is 80-120 mesh;

[0028] The specific steps of the subcritical treatment in step (5) are: placing the phospholipid DHA microcapsule powder in a subcritical equipment extraction tank, adding an alkane solvent according to a certain material-liquid ratio, stirring for a certain time under certain pressure conditions, then separating the phospholipid DHA microcapsules from the solvent, recovering the solvent, and sieving the phospholipid DHA microcapsule powder treated with subcritical fluid;

[0029] The solid-liquid ratio (g / mL) of the phospholipid DHA microcapsule powder and the alkane solvent is 1:1-10, preferably 1:3-5, the operating pressure is 0.3-0.7 MPa, the stirring time is 10-60 minutes, and after the solvent is recovered, the 80-120 mesh powder is collected by sieving.

[0030] Preferably, the alkane solvent is propane or butane.

[0031] A stabilized phospholipid DHA microcapsule is prepared by the above method.

[0032] The stabilized phospholipid DHA microcapsules are used in the preparation of nutritional supplements, health products and functional foods.

[0033] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0034] 1. The present invention adds a polysaccharide substance with a molecular weight of less than 10,000 Daltons into the embedding material, which can improve the stability of the polyunsaturated fatty acid oil.

[0035] 2. In the present invention, after the unsaturated fatty acid oil microcapsule powder is subjected to subcritical treatment, under the action of the subcritical operating pressure, the oil in the outer wall structure that is not dense enough and the grease in the shell structure cavity is eluted, and the volume of this part of the unsaturated fatty acid oil microcapsule powder is compressed to a smaller size, which can be removed by screening later.

[0036] 3. The phospholipid DHA microcapsules prepared by the present invention have no surface oil, complete structure, good taste masking, no fishy smell or fishy smell after being placed for 3 months, the peroxide value only increases from 0.37 mmol / kg to 0.69-0.73 mmol / kg, and the malondialdehyde content only increases from 2.8 ppm to 5.58-6.23 ppm, which are lower than the peroxide value (0.84-1.66 mmol / kg) and malondialdehyde content (10.44-18.92 ppm) of the microcapsule powder prepared in the comparative example, and much lower than the peroxide value (2.34 mmol / kg) and malondialdehyde content (23.3 ppm) of the raw material, and have excellent storage stability. DETAILED DESCRIPTION

[0037] The present invention is further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto. For process parameters not particularly noted, conventional techniques may be used.

[0038] Resistant dextrin, soy protein isolate, phospholipid DHA oil, polysaccharides, all of which are commercially available.

[0039] Example 1

[0040] Weigh 20g of resistant dextrin and 20g of soy protein isolate, mix them evenly, add water and stir to dissolve into a wall material solution with a mass concentration of 10%; add 4g of lentinan with a molecular weight less than 1000 to the prepared wall material solution, and use a disperser to disperse until the polysaccharide is completely dissolved; then add 40g of phospholipid DHA oil with a DHA content of 56wt.%, the mass ratio of the core material oil to the embedded wall material is 1:1, use a disperser at 10000r / min to disperse for 15min, and then homogenize under the following conditions: homogenization pressure of 35MPa, homogenization temperature of 10°C, homogenize 3 times to prepare a uniform emulsion; freeze-dry the emulsion, after drying is completed, crush it, sieve the product and collect 80-120 mesh as the target microcapsule powder.

[0041] Weigh 50g of the target microcapsule powder and place it in a subcritical equipment extraction tank, add 150mL of butane solvent, the solid-liquid ratio is 1:3, maintain the temperature of the extraction tank at 40°C and the pressure at 0.7MPa and stir for 20 minutes, then separate the solvent from the microcapsule powder, recover the solvent, and sieve the subcritically treated powder to collect 80-120 mesh to obtain stabilized phospholipid DHA microcapsule sample 1.

[0042] Example 2

[0043] Weigh 25g of resistant dextrin and 15g of soy protein isolate, mix them evenly, add water and stir to dissolve into a wall material solution with a mass concentration of 10%; add 4g of Poria cocos polysaccharide with a molecular weight less than 1000 to the prepared wall material solution, and use a disperser to disperse until the polysaccharide is completely dissolved; then add 48g of phospholipid DHA oil with a DHA content of 56wt.%, the mass ratio of the core material oil to the embedded wall material is 1.2:1, use a disperser at 10000r / min to disperse for 15min, and then homogenize under the following conditions: homogenization pressure of 35MPa, homogenization temperature of 10℃, homogenize 3 times to prepare a uniform emulsion; freeze-dry the emulsion, after drying is completed, crush it, sieve the product and collect 80-120 mesh as the target microcapsule powder.

[0044] Weigh 50g of target microcapsule powder and place it in a subcritical equipment extraction tank, add 200mL of butane solvent, the solid-liquid ratio is 1:4, maintain the pressure and temperature of the extraction tank at 45°C and 0.7MPa and stir for 20 minutes, then separate the solvent from the microcapsule powder, recover the solvent, and sieve the subcritically treated powder to collect 80-120 mesh to obtain stabilized phospholipid type DHA microcapsule sample 2.

[0045] Example 3

[0046] Weigh 30g of resistant dextrin and 10g of soy protein isolate, mix them evenly, add water and stir to dissolve into a wall material solution with a mass concentration of 20%; add 2g of Hericium erinaceus polysaccharide with a molecular weight of less than 500 to the prepared wall material solution, and use a disperser to disperse until the polysaccharide is completely dissolved; then add 48g of phospholipid DHA oil with a DHA content of 56wt.%, the mass ratio of the core material oil to the embedded wall material is 1.2:1, use a disperser at 10000r / min to disperse for 18min, and then homogenize under the following conditions: homogenization pressure of 35MPa, homogenization temperature of 2°C, homogenize 3 times to prepare a uniform emulsion; the emulsion is freeze-dried, and after drying is completed, it is crushed, and the product is sieved and collected to take 80-120 mesh as the target microcapsule powder.

[0047] Weigh 50g of the target microcapsule powder and place it in a subcritical equipment extraction tank, add 250mL of propane solvent, the solid-liquid ratio is 1:5, maintain the temperature of the extraction tank at 35°C and the pressure at 0.5MPa and stir for 30 minutes, then separate the solvent from the microcapsule powder, recover the solvent, and sieve the subcritically treated powder to collect 80-120 mesh to obtain stabilized phospholipid type DHA microcapsule sample 3.

[0048] Example 4

[0049] 30g of resistant dextrin and 10g of soy protein isolate are weighed, mixed evenly, and then water is added to stir and dissolve to form a wall material solution with a mass concentration of 10%; 8g of Poria cocos polysaccharide with a molecular weight less than 500 is added to the prepared wall material solution, and a disperser is used to disperse the polysaccharide until the polysaccharide is completely dissolved; then 60g of phospholipid DHA oil with a DHA content of 56wt.% is added, and the mass ratio of the core material oil to the embedded wall material is 1.5:1, and the dispersion machine is used to disperse for 20min at 10000r / min, and then the homogenization conditions are: homogenization pressure 30MPa, homogenization temperature 10℃, homogenization 3 times, to prepare a uniform emulsion; the emulsion is freeze-dried, and after drying is completed, it is crushed, and the product is sieved and collected to take 80-120 mesh as the target microcapsule powder.

[0050] Weigh 50g of target microcapsule powder and place it in a subcritical equipment extraction tank, add 200mL of propane solvent, the solid-liquid ratio is 1:4, maintain the pressure and temperature of the extraction tank at 35°C and 0.5MPa and stir for 25 minutes, then separate the solvent from the microcapsule powder, recover the solvent, and sieve the subcritically treated powder to collect 80-120 mesh to obtain stabilized phospholipid type DHA microcapsule sample 4.

[0051] Example 5

[0052] 10g of resistant dextrin and 30g of soy protein isolate are weighed, mixed evenly, and then water is added to stir and dissolve to form a wall material solution with a mass concentration of 10%; 6g of lentinan with a molecular weight less than 1000 is added to the prepared wall material solution, and a disperser is used to disperse the polysaccharide until the polysaccharide is completely dissolved; then 48g of phospholipid DHA oil with a DHA content of 56wt.% is added, and the mass ratio of the core material oil to the embedded wall material is 1.2:1, and the disperser is used to disperse at 10000r / min for 15min, and then the homogenization conditions are: homogenization pressure 35MPa, homogenization temperature 10℃, homogenization 3 times, to prepare a uniform emulsion; the emulsion is freeze-dried, and after drying is completed, it is crushed, and the product is sieved and collected to take 80-120 mesh as the target microcapsule powder.

[0053] Weigh 50g of the target microcapsule powder and place it in a subcritical equipment extraction tank, add 250mL of butane solvent, the solid-liquid ratio is 1:5, maintain the pressure of the extraction tank at 0.7MPa and stir for 30 minutes, then separate the solvent from the microcapsule powder, recover the solvent, and sieve the subcritically treated powder to collect 80-120 mesh to obtain stabilized phospholipid type DHA microcapsule sample 5.

[0054] Comparative Example 1

[0055] Compared with Example 1, no polysaccharide was added, and other parameters and methods were the same, to obtain a phospholipid type DHA microcapsule comparison sample 1.

[0056] Comparative Example 2

[0057] Compared with Example 1, no subcritical fluid equipment treatment was performed, and other parameters and methods were the same, thereby obtaining a phospholipid type DHA microcapsule comparative sample 2.

[0058] Comparative Example 3

[0059] Compared with Example 1, maltodextrin was used instead of resistant dextrin, and other parameters and methods were the same to obtain phospholipid DHA microcapsule comparison sample 3.

[0060] Comparative Example 4

[0061] Compared with Example 1, other parameters and methods were the same, the solid-liquid ratio was 1:7, and a phospholipid type DHA microcapsule comparison sample 4 was obtained.

[0062] Comparative Example 5

[0063] Compared with Example 1, the molecular weight of the added polysaccharide is greater than 1000, and other parameters and methods are the same, to obtain phospholipid DHA microcapsule comparison sample 5.

[0064] Test Case

[0065] The microcapsules obtained in the examples and comparative examples were evaluated for fishy smell intensity and stability tests were performed. The stability test conditions were: at 37° C. and 75% relative humidity for 3 months, and the peroxide value and malondialdehyde content were measured.

[0066] Evaluation of fishy smell intensity: The fishy smell intensity is scored on a 7-point scale (0 for absence, 1 for presence, 2 for mild, 3 to 4 for moderate, 4 to 5 for heavy, and 7 for very heavy). The average score of the 30 evaluators is the fishy smell intensity.

[0067] Peroxide value determination: refer to GB 5009.227-2023 "National Food Safety Standard Determination of Peroxide Value in Food"

[0068] Determination of malondialdehyde content: refer to GB 5009.181-2016 "National Food Safety Standard Determination of Malondialdehyde in Food", the details are as follows:

[0069] The method for detecting the malondialdehyde content of polyunsaturated fatty acid oil raw materials is as follows: (1) Take 2 g of phospholipid type DHA oil sample in a conical flask, add 50 mL of TCA, shake for 30 minutes, and filter to obtain a clear liquid; (2) Take 5 mL of the clear liquid in a 25 mL colorimetric tube, add 5 mL of TBA, incubate at 90°C for 20 minutes, transfer to a centrifuge tube, centrifuge at 12000 r / min for 5 minutes, take the supernatant for high performance liquid chromatography detection, and treat the malondialdehyde standard in the same way.

[0070] The method for detecting the malondialdehyde content in microcapsule powder is as follows: (1) Weigh 30 g of microcapsule powder sample and add it to a separatory filter; shake gently to disperse the powder in water, but not completely dissolve it. Add 120 mL of petroleum ether and shake it thoroughly to make it completely emulsified; add 150 mL of ethanol to the emulsion along the glass wall and shake it gently several times to mix the ethanol into it, let it stand to separate; take the upper organic layer and desolventize it on a rotary evaporator; (2) Take 2 g of the desolventized oil sample in a conical flask, add 50 mL of TCA, shake for 30 minutes, and filter to obtain a clear liquid; (3) Take 5 mL of the clear liquid in a 25 mL colorimetric tube, add 5 mL of TBA, incubate at 90°C for 20 minutes, transfer to a centrifuge tube, centrifuge at 12000 r / min for 5 minutes, take the supernatant for HPLC detection, and the malondialdehyde standard is treated in the same way.

[0071] Surface oil content detection: weigh 2g of microcapsule powder sample, add 25mL petroleum ether, evenly disperse the microcapsule powder sample in the petroleum ether, and shake manually for 3 minutes without destroying the microcapsules. Then, filter the solvent through a Buchner funnel, rinse the collected microcapsule powder sample with 15mL petroleum ether three times, and use a rotary evaporator to evaporate and remove the solvent in the filtrate to obtain the surface oil content.

[0072] The results of the fishy smell intensity evaluation test are shown in Table 1:

[0073] Table 1

[0074] sample initial 37℃, 75%RH, 3 months later Example 1 0 0 Example 2 0 1 Example 3 0 0 Example 4 0 0 Example 5 0 1 Comparative Example 1 1 2 Comparative Example 2 3 5 Comparative Example 3 1 3 Comparative Example 4 2 5 Comparative Example 5 2 4

[0075] The results of peroxide value and malondialdehyde determination are shown in Table 2:

[0076] Table 2

[0077]

[0078] The results of surface oil content determination are shown in Table 3:

[0079] Table 3

[0080] sample Surface oil content g / g Example 1 0 Example 2 0 Example 3 0 Example 4 0 Example 5 0 Comparative Example 1 0 Comparative Example 2 0.13 Comparative Example 3 0 Comparative Example 4 0 Comparative Example 5 0

[0081] The surface oil content of the stabilized phospholipid DHA microcapsules prepared in the embodiment of the present invention was 0, and there was no fishy smell or fishy smell after being placed for 3 months. The peroxide value and malondialdehyde content of the oil in the microcapsule powder did not change much, and the microcapsule powder had good stability and could be stored for a long time.

[0082] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A method for preparing stabilized phospholipid DHA microcapsules, characterized in that: The steps include: (1) Weighing resistant dextrin and soy protein isolate as embedding wall materials in proportion, stirring evenly, adding water and stirring to dissolve into a wall material solution; (2) adding polysaccharide to the wall material solution, stirring and dispersing until the polysaccharide is completely dissolved, to obtain solution A; (3) adding the core material oil to solution A in a certain proportion, stirring and dispersing, and then homogenizing by high pressure to prepare an emulsion; (4) drying and sieving the stable emulsion prepared in step (3) to prepare phospholipid-type DHA microcapsule powder; (5) The phospholipid DHA microcapsule powder prepared in step (4) is subjected to subcritical treatment and sieved to obtain stabilized phospholipid DHA microcapsule powder.

2. The method for preparing stabilized phospholipid DHA microcapsules according to claim 1, characterized in that: The mass ratio of the resistant dextrin to the isolated soy protein is 1-3:1-3; The mass concentration of the wall material solution in step (1) is 5%-30%.

3. The method for preparing stabilized phospholipid DHA microcapsules according to claim 1, characterized in that: The polysaccharide is one or more of plant polysaccharides or microbial polysaccharides, and the molecular weight of the polysaccharide is less than 10,000 Daltons.

4. The method for preparing stabilized phospholipid DHA microcapsules according to claim 1, characterized in that: The added amount of the polysaccharide is 5%-20% of the weight of the embedding wall material.

5. The method for preparing stabilized phospholipid DHA microcapsules according to claim 1, characterized in that: The mass ratio of core material grease to embedded wall material is 1-2:

1.

6. The method for preparing stabilized phospholipid DHA microcapsules according to claim 1, characterized in that: The core material oil is phospholipid-type DHA, which is derived from one or a mixture of soybean lecithin or krill oil lecithin, and is prepared by catalysis of an immobilized enzyme, wherein the DHA content is 40-70wt.%.

7. The method for preparing stabilized phospholipid DHA microcapsules according to claim 1, characterized in that: The conditions of high pressure homogenization in step (3) are: homogenization pressure of 20-50 MPa, homogenization times of 2-7 times, and homogenization temperature of 0-20°C; The drying in step (4) is spray drying or freeze drying, and the phospholipid DHA microcapsule powder is 80-120 mesh.

8. The method for preparing stabilized phospholipid DHA microcapsules according to claim 1, characterized in that: The specific steps of the subcritical treatment in step (5) are: placing the phospholipid DHA microcapsule powder in a subcritical equipment extraction tank, adding an alkane solvent according to a certain material-liquid ratio, stirring for a certain time under certain pressure conditions, then separating the phospholipid DHA microcapsules from the solvent, recovering the solvent, and sieving the phospholipid DHA microcapsule powder treated with subcritical fluid; The solid-liquid ratio of the phospholipid DHA microcapsule powder to the alkane solvent is 1:1-10, the operating pressure is 0.3-0.7 MPa, the stirring time is 10-60 minutes, and after the solvent is recovered, the 80-120 mesh powder is collected by sieving.

9. A stabilized phospholipid DHA microcapsule, characterized in that: The method is prepared by any one of claims 1 to 8.

10. Use of the stabilized phospholipid DHA microcapsules according to claim 9 in the preparation of nutritional supplements, health products and functional foods.

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