Lip care sunscreen microcapsule, preparation method and application of microcapsule in preparation of sunscreen activating lip balm

Abstract

The invention discloses a lip care sunscreen microcapsule, a preparation method and application of the lip care sunscreen microcapsule in preparation of a sunscreen activating lip balm. The lip care sunscreen microcapsule comprises prodigiosin, astaxanthin, pyrroloquinoline quinone, candelilla wax, shea butter, lauryl alcohol laurate, zein, hydrolyzed corn starch octenyl succinate, silica and benzyl ether myristate. Bioactive substances with excellent anti-allergy performance, namely prodigiosin / astaxanthin / pyrroloquinoline quinone, are screened through a synthetic biological technology, and the substances can regulate the immune response of skin, relieve inflammation and enhance the barrier function of the skin, so that the allergic reaction of sensitive skin to ultraviolet rays is effectively reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of microcapsule application, and in particular relates to a lip care sunscreen microcapsule and a preparation method thereof, and application thereof in the preparation of a sunscreen activating lip balm. Background Art

[0002] In the field of lip care, there are many types of lip products on the market; among them, lip care products mainly focus on moisturizing and color modification. Common lip balm products usually have main ingredients including vaseline, lanolin, natural oils (such as jojoba oil, coconut oil), etc. These ingredients can form a protective film on the surface of the lips, reduce the evaporation of lip moisture, and thus keep the lips moist. For example, some well-known brands of lip balm can relieve dry lips and peeling in a short time. For lip products with color functions, such as lipsticks, lip glosses, etc., in addition to adding pigment ingredients, they also contain certain moisturizing ingredients, which can not only give the lips color, but also play a certain repairing role.

[0003] In terms of sun protection, there are many types of facial sunscreen products. The sun protection principle is mainly to absorb or reflect ultraviolet rays by adding organic sunscreens (such as para-aminobenzoic acid and its derivatives, cinnamic acid esters, etc.) and inorganic sunscreens (such as titanium dioxide, zinc oxide). These sunscreens can effectively resist UVA and UVB in ultraviolet rays and protect facial skin from damage such as photoaging and sunburn. However, these facial sunscreen products are not specifically designed for lips. The texture of facial sunscreen products is usually thicker, and there will be obvious discomfort when applied to the lips, affecting the touch and appearance of the lips. Although some lipsticks or lip glosses containing sunscreen ingredients take into account the sunscreen and color functions to a certain extent, the sun protection index is often low, and they cannot provide sufficient ultraviolet protection, and they are easy to destroy the original lip makeup when reapplying. In addition, these products are also insufficient in terms of sunscreen durability. When outdoor activities are carried out for a long time, they cannot continuously and effectively protect the lips from ultraviolet damage. At the same time, the color problem must be met. Red is a popular color.

[0004] Prodigiosin is a tripyrrole ring structure with a maximum absorption wavelength of 530nm, which is synthesized by bacteria such as Serratia marcescens. Studies by Suryawanshi et al. show that prodigiosin can be used as an additive in sunscreen products due to its photosensitivity, thereby reducing the damage of ultraviolet rays to the skin. However, if prodigiosin is used alone as a sunscreen, it cannot produce a meaningful SPF value, but it can increase the SPF value of sunscreen products or simple plant extracts.

[0005] Astaxanthin is a natural pigment with strong antioxidant capacity. It mainly exists in some marine organisms in nature, such as shrimps and crabs. Astaxanthin can absorb ultraviolet rays, especially long-wave ultraviolet rays (UVA), which can reduce the damage of UVA to the skin. At the same time, the antioxidant effect of astaxanthin can remove free radicals generated by ultraviolet radiation and prevent free radicals from damaging skin cells, thereby playing a role in sun protection and skin protection.

[0006] Pyrroloquinoline quinone (PQQ) has maximum absorption at a wavelength of 250nm and can effectively prevent photoaging caused by ultraviolet rays. Currently, the active ingredients in the prior art are mostly encapsulated in oil-in-water emulsions, which are not easy to add to lip products. Summary of the invention

[0007] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0008] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed.

[0009] Therefore, the object of the present invention is to overcome the deficiencies in the prior art and provide a lip care sunscreen multi-chamber microcapsule.

[0010] In order to solve the above technical problems, the present invention provides the following technical solutions: a lip care sunscreen multi-chamber microcapsule, comprising prodigiosin, astaxanthin, pyrroloquinoline quinone, candelilla wax, shea butter, lauryl laurate, zein, hydrolyzed corn starch octenyl succinate, silica and benzyl ether myristate;

[0011] Calculated by the mass percentage of the raw materials, the prodigiosin is 0.1% to 5%, the astaxanthin is 0.1% to 5%, the pyrroloquinoline quinone is 0.1% to 5%, the candelilla wax is 5% to 20%, the shea butter is 5% to 20%, the lauryl laurate is 5% to 10%, the zein is 1% to 5%, the hydrolyzed corn starch octenyl succinate is 20% to 40%, the silica is 10% to 20%, and the benzyl ether myristate is 5% to 10%.

[0012] As a preferred embodiment of the lip care sunscreen multi-chamber microcapsules of the present invention, the multi-chamber microcapsules achieve enzyme-controlled release.

[0013] Another object of the present invention is to overcome the deficiencies in the prior art and provide a method for preparing lip care sunscreen multi-chamber microcapsules, comprising:

[0014] Heat prodigiosin, astaxanthin, pyrroloquinoline quinone, candelilla wax and shea butter until they are completely miscible to form phase A;

[0015] Heat lauryl laurate, benzyl ether myristate and water until they are completely miscible to form phase B;

[0016] Slowly add phase A into phase B at 80°C and stir evenly, then homogenize under high pressure at 800 bar to obtain phase C;

[0017] Zein and hydrolyzed corn starch octenyl succinate were dissolved in deionized water and hydrated overnight to obtain a transparent liquid, which was then heated in a water bath at 90° C. with stirring, and silica was added and stirred to obtain phase D;

[0018] Mix phase C and phase D, stir evenly, put them into a micro high-pressure atomizing pump, use an open container to hold liquid nitrogen, then aim the atomizing pump nozzle at the liquid nitrogen and start the pump to spray;

[0019] After spraying, the open container is placed in a freeze dryer and vacuum dried to obtain multi-chamber microcapsules.

[0020] As a preferred embodiment of the preparation method of the present invention, the heating temperature during the preparation of phase A is 80°C.

[0021] As a preferred embodiment of the preparation method of the present invention, the heating temperature during the preparation of phase B is 80°C.

[0022] As a preferred embodiment of the preparation method of the present invention, the number of high pressure homogenization times in the preparation process of phase C is 3 to 5 times.

[0023] As a preferred embodiment of the preparation method of the present invention, the nozzle of the injection is 0.2 mm.

[0024] Another object of the present invention is to overcome the deficiencies in the prior art and provide a lip care sunscreen multi-chamber microcapsule for use in the preparation of a sunscreen activating lip balm.

[0025] As a preferred embodiment of the application of the present invention, the sunscreen activating lip balm comprises the following components in mass fractions:

[0026] Lip care sunscreen multi-chamber microcapsule 10%~30%, microcrystalline wax 10%~30%, mica 10%~15%, walnut seed oil 2%~10%, isononyl isononanoate 2%~10%, jojoba seed oil 2%~10%, polyglyceryl-3-diisostearate 2%~5%, hydroxypropyl tetrahydropyrantriol 0.1%~2%, sodium hyaluronate 0.1%~2%, glucomannan 0.1%~2%, water is added to 100%.

[0027] As a preferred embodiment of the application of the present invention, the preparation method of the sunscreen revitalizing lip balm comprises:

[0028] Mixing microcrystalline wax, mica, walnut seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate, stirring, dissolving, heating, and stirring until uniformly dissolved, to prepare a first phase;

[0029] Hydroxypropyl tetrahydropyrantriol, sodium hyaluronate, glucomannan and water are mixed, stirred, dissolved, heated and stirred until uniformly dissolved to prepare a second phase;

[0030] Mix the first phase and the second phase and stir evenly, add the lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour it into a mold or barrel to form and discharge, and prepare a sunscreen revitalizing lip balm.

[0031] Beneficial effects of the present invention:

[0032] (1) The present invention uses synthetic biology technology to screen out a combination of bioactive substances with excellent anti-allergic properties, namely prodigiosin / astaxanthin / pyrroloquinoline quinone. These substances can regulate the immune response of the skin, reduce inflammation, and enhance the skin barrier function, thereby effectively reducing the allergic reaction of sensitive skin to ultraviolet rays.

[0033] (2) The present invention adopts nanotechnology and low-temperature spray freezing technology to prepare multi-chamber microcapsules, and the multi-chamber microcapsules can achieve enzyme-controlled release.

[0034] (3) The present invention prepares a sunscreen activating lip balm, which can achieve the gradual release of functional components and has the functions of anti-aging, sunscreen and self-coloring. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0036] Figure 1 This is a cryo-TEM structure diagram of the sample in Example 1 of the present invention.

[0037] Figure 2 This is a time curve of active substance release in Example 2 of the present invention.

[0038] Figure 3 This is a graph showing the anti-aging test results of the sample in Example 4 of the present invention.

[0039] Figure 4 This is a diagram showing the sun protection test results of the samples in Example 4 of the present invention. DETAILED DESCRIPTION

[0040] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0041] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0042] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.

[0043] The raw materials used in the embodiments of the present invention are all common commercially available products.

[0044] Example 1

[0045] Microcapsules were prepared according to the raw material ratio in Table 1:

[0046] (1) heating prodigiosin, astaxanthin, pyrroloquinoline quinone, candelilla wax, and shea butter at 80° C. until they are completely miscible to form phase A;

[0047] (2) heating lauryl laurate, PPG-3 benzyl ether myristate and water at 80° C. until they are completely miscible to form phase B;

[0048] (3) Phase A was slowly added to phase B at 80°C and stirred evenly, and then high-pressure homogenization was performed for 5 times at 800 bar to obtain phase C.

[0049] (4) Zein and hydrolyzed corn starch octenyl succinate were dissolved in deionized water and hydrated overnight to obtain a transparent liquid. The liquid was then heated in a water bath at 90° C. with stirring, and silica was added and stirred to obtain a phase D.

[0050] (5) Mix phase C and phase D, stir evenly, load into a micro high-pressure atomizing pump, use an open container to hold liquid nitrogen, then aim the atomizing pump nozzle at the liquid nitrogen, start the pump, and use a 0.2 mm nozzle.

[0051] After spraying, the open container was placed in a freeze dryer and vacuum dried to obtain powder.

[0052] Table 1 Raw material ratio table (unit: g)

[0053]

[0054] The prepared microcapsules will be dispersed in the oil solution without precipitation, and can form a uniform liquid at 80°C. Microcapsules formed by incorrectly proportioned compositions cannot form microcapsules, cannot be uniformly dispersed in the oil solution, and powder will precipitate.

[0055] The structure of sample 1 was characterized by scanning electron microscopy. Figure 1 .

[0056] from Figure 1 It can be seen that the microcapsule prepared by sample 1 is a microsphere with a multi-chamber structure.

[0057] Example 2

[0058] Weigh an appropriate amount of sample 1 in Example 1, add different amounts of α-amylase to make small pieces with a diameter of 10 mm, each piece containing about 20 mg of prodigiosin.

[0059] A drug dissolution apparatus was used with the paddle method, 500 mL of pH 7.4 phosphate buffer (10% methanol) was used as the dissolution medium, the temperature was (37±0.5)°C, and the rotation speed was 100 r / min.

[0060] 5.0 mL of sample was taken every 1 hour, and 5.0 mL of dissolution medium at the same temperature was added to the release system. After filtering with a 0.22 μm microporous filter membrane, the filtrate was appropriately diluted with 10% methanol solution, and the content of prodigiosin was determined by high performance liquid chromatography.

[0061] The cumulative release rate of the active substance is calculated as follows:

[0062]

[0063] Where, W 0 is the active ingredient content (mg), Vt is the medium volume, Vs is the sampling volume, ci is the concentration of the active ingredient in the release liquid at the i-th measurement at time t, and ∑ci-1 is the sum of the active ingredient concentrations measured in the previous i-1 times.

[0064] The effect of α-amylase on the controlled release of active substances in microcapsules was investigated. The relationship between the cumulative release rate and time of active substances in microcapsules at different enzyme contents was tested. The results are as follows: Figure 2 As shown;

[0065] It can be seen from the figure that the higher the enzyme content, the faster the active substance is released. When there is no enzyme, the microcapsules only produce a trace amount of release due to temperature.

[0066] Example 3

[0067] (1) Sunscreen Revitalizing Lip Balm Formula (the raw materials used are all conventional cosmetic raw materials, purchased from cosmetic raw material companies such as Shanghai Puen Biochemical Co., Ltd. and Shanghai Coin Co., Ltd.):

[0068] Lip care sunscreen multi-chamber microcapsules 20% (sample 1 in Example 1);

[0069] Microcrystalline wax 25%;

[0070] Mica 10%;

[0071] Juglans regia seed oil 8%;

[0072] Isononyl isononanoate 8%;

[0073] Jojoba seed oil 8%;

[0074] Polyglyceryl-3-diisostearate 4%;

[0075] Hydroxypropyl tetrahydropyrantriol 1%;

[0076] Sodium hyaluronate 0.1%;

[0077] Glucomannan 1%;

[0078] Make up to 100% with water.

[0079] (2) Preparation method of sunscreen revitalizing lip balm: mix microcrystalline wax, mica, walnut seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate, stir and dissolve, heat and stir to 82° C. to disperse / dissolve evenly;

[0080] Mix hydroxypropyl tetrahydropyrantriol, sodium hyaluronate, glucomannan and water, stir and dissolve, heat and stir to 82°C to disperse / dissolve evenly, and mix and stir the two items evenly;

[0081] Add lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour it into a mold or barrel for molding and discharging, and prepare a sunscreen activating lip balm.

[0082] Comparative Example 1

[0083] (1) Sunscreen Revitalizing Lip Balm Ingredients:

[0084] Lip care sunscreen multi-chamber microcapsules 20% (sample 2 in Example 1, containing no active ingredient);

[0085] Microcrystalline wax 25%;

[0086] Mica 10%;

[0087] Juglans regia seed oil 8%;

[0088] Isononyl isononanoate 8%;

[0089] Jojoba seed oil 8%;

[0090] Polyglyceryl-3-diisostearate 4%;

[0091] Hydroxypropyl tetrahydropyrantriol 1%;

[0092] Sodium hyaluronate 0.1%;

[0093] Glucomannan 1%;

[0094] Make up to 100% with water.

[0095] (2) Preparation method: Mix microcrystalline wax, mica, walnut seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate, stir and dissolve, heat and stir until the mixture is uniformly dispersed / dissolved at 82°C;

[0096] Mix hydroxypropyl tetrahydropyrantriol, sodium hyaluronate, glucomannan and water, stir to dissolve, heat and stir to 82°C to disperse / dissolve evenly, mix the two and stir evenly, add lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour into a mold or barrel to form and discharge, and make a sunscreen revitalizing lip balm.

[0097] Comparative Example 2

[0098] (1) Sunscreen Revitalizing Lip Balm:

[0099] Lip care sunscreen multi-chamber microcapsules 20% (sample 8 in Example 1);

[0100] Microcrystalline wax 25%;

[0101] Mica 10%;

[0102] Juglans regia seed oil 8%;

[0103] Isononyl isononanoate 8%;

[0104] Jojoba seed oil 8%;

[0105] Polyglyceryl-3-diisostearate 4%;

[0106] Hydroxypropyl tetrahydropyrantriol 1%;

[0107] Sodium hyaluronate 0.1%;

[0108] Glucomannan 1%;

[0109] Make up to 100% with water.

[0110] (2) Preparation method: Mix microcrystalline wax, mica, walnut seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate, stir and dissolve, heat and stir until the mixture is uniformly dispersed / dissolved at 82°C;

[0111] Mix hydroxypropyl tetrahydropyrantriol, sodium hyaluronate, glucomannan and water, stir to dissolve, heat and stir to 82°C to disperse / dissolve evenly; stir the two mixtures evenly, add lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour into a mold or barrel to form and discharge, and prepare a sunscreen revitalizing lip balm.

[0112] Comparative Example 3

[0113] (1) Sunscreen Revitalizing Lip Balm:

[0114] Lip care sunscreen multi-chamber microcapsules 20% (sample 9 in Example 1);

[0115] Microcrystalline wax 25%;

[0116] Mica 10%;

[0117] Juglans regia seed oil 8%;

[0118] Isononyl isononanoate 8%;

[0119] Jojoba seed oil 8%;

[0120] Polyglyceryl-3-diisostearate 4%;

[0121] Hydroxypropyl tetrahydropyrantriol 1%;

[0122] Sodium hyaluronate 0.1%;

[0123] Glucomannan 1%;

[0124] Make up to 100% with water.

[0125] (2) Preparation method: Mix microcrystalline wax, mica, walnut seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate, stir and dissolve, heat and stir until the mixture is uniformly dispersed / dissolved at 82°C;

[0126] Mix hydroxypropyl tetrahydropyrantriol, sodium hyaluronate, glucomannan and water, stir to dissolve, heat and stir to 82°C to disperse / dissolve evenly;

[0127] Mix the two ingredients evenly, add the lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour it into a mold or barrel to form and discharge the material, and prepare the sunscreen revitalizing lip balm.

[0128] Comparative Example 4

[0129] (1) Sunscreen Revitalizing Lip Balm:

[0130] Lip care sunscreen multi-chamber microcapsules 20% (sample 10 in Example 1);

[0131] Microcrystalline wax 25%;

[0132] Mica 10%;

[0133] Juglans regia seed oil 8%;

[0134] Isononyl isononanoate 8%;

[0135] Jojoba seed oil 8%;

[0136] Polyglyceryl-3-diisostearate 4%;

[0137] Hydroxypropyl tetrahydropyrantriol 1%;

[0138] Sodium hyaluronate 0.1%;

[0139] Glucomannan 1%;

[0140] Make up to 100% with water.

[0141] (2) Preparation method: Mix microcrystalline wax, mica, walnut seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate, stir and dissolve, heat and stir until the mixture is uniformly dispersed / dissolved at 82°C;

[0142] Mix hydroxypropyl tetrahydropyrantriol, sodium hyaluronate, glucomannan and water, stir to dissolve, heat and stir to 82°C to disperse / dissolve evenly;

[0143] Mix the two ingredients evenly, add the lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour it into a mold or barrel to form and discharge the material, and prepare the sunscreen revitalizing lip balm.

[0144] Example 4

[0145] Moisturizing effect:

[0146] Samples: Example 3, Comparative Example 1, commercially available samples.

[0147] Purpose of test: Single-center open trial, through the method of subjective evaluation by more than 30 people after using the product, with before and after comparison, to verify the efficacy of the product.

[0148] Experimenters: This test included 30 healthy female subjects aged 20-50 years old. Volunteers with slight fine lines, dry lines or obvious wrinkles on the lips were preferred. The subjects were evenly divided into three groups, and three samples were tested in each group.

[0149] Test method:

[0150] Before and after comparison, the volunteers' lips were tested before using the product, 8 hours after using the product, 7 days after using the product, and 14 days after using the product. The test was for the water content of the lip stratum corneum.

[0151] How to use the sample: Apply the sample to the lips once a day, evenly applying it on the lips.

[0152] Test equipment:

[0153] Multi-probe skin testing system (Courage & Khazaka, Germany): Moisture Corneometer probe to measure moisture content.

[0154] Skin stratum corneum water content (au) change rate (%) = (measured value - initial value) / initial value × 100%

[0155] See the table below for specific results.

[0156] Change rate of skin stratum corneum water content (au) 8H 7 days 14 days Example 3 7.24％ 14.43％ 12.43％ Comparative Example 1 3.47％ 9.36％ 7.13％ Commercially available products 5.37％ 10.45％ 8.91％

[0157] The water content of the stratum corneum of the skin is a common indicator reflecting the moisturizing effect. The data in the above table show that the components of the present invention have good skin moisturizing ability.

[0158] Barrier repair effect:

[0159] Samples: Example 3, Comparative Example 1, commercially available samples.

[0160] Purpose of test: Single-center open trial, using instrument testing method with more than 30 people using the product, with before and after comparison, to verify the efficacy of the product.

[0161] Experimenters: This test included 30 healthy female subjects aged 20-50 years old. Volunteers with slight fine lines, dry lines or obvious wrinkles on the lips were preferred. The subjects were evenly divided into three groups, and three samples were tested in each group.

[0162] Test method:

[0163] Before and after comparison, the volunteers' lips were tested before use of the product, 8 hours after use of the product, 7 days after use of the product, and 14 days after use of the product. The test was the transepidermal water loss of the skin. Sample usage: The tester applies the sample to the lips once a day.

[0164] Test equipment:

[0165] Multi-probe skin testing system (Courage & Khazaka, Germany): Transcutaneous water loss Tewameter probe.

[0166] The test results are as follows:

[0167] Changes in skin TEWL value 8H Difference 7D Difference 14 days difference Example 3 -15 -17 -21 Comparative Example 1 -11 -13 -15 Commercially available products -7 -8 -12

[0168] Transepidermal water loss (TEWL), also known as transdermal water loss, is a common indicator of the barrier function of the stratum corneum. If the skin keratin is abnormal, damaged, or poorly cared for, the skin barrier will be damaged, causing the skin to lose its protective and moisturizing functions.

[0169] The data in the above table show that the components of the present invention have good skin repair ability, can significantly reduce transepidermal water loss and repair the skin.

[0170] Example 5

[0171] Anti-aging function:

[0172] Samples: Example 3, Comparative Examples 1, 2, 3, 4

[0173] This test uses human skin fibroblasts (HFF-1 cells) as model cells to measure the migration ability of samples to fibroblasts damaged by UVB.

[0174] HFF-1 cells were cultured at 2.0×10 5 The cells were plated at a density of 100 μg / mL and 70 μL per well was inoculated into 12-well culture-inserts (2-well, ibidi). After culturing for 24 h in complete medium, the medium was removed and the cells were washed three times with PBS.

[0175] Then 100 μL of PBS buffer was added to each well, and the sample (20 mg / cm 2 ) was evenly spread on the lid of a 12-well plate, the blank group was covered with tin foil, and the model group was untreated.

[0176] Then, the samples were irradiated with UVB (308 nm) for 5 min, with a total energy of 40 mJ / cm 2 The supernatant was removed and replaced with DMEM medium containing 5% FBS, and the culture was continued for 24 h. The images were taken and the scratch area was calculated.

[0177] Results Figure 3, indicating that after UVB irradiation, the model group had weak migration ability, but the blank group without UVB stimulation had a certain degree of closure of the scratch area after 24 hours of culture. The sample of Example 3 also had cell migration ability compared with the model group, with a significance of **P<0.001.

[0178] There was no significant difference between the sample group of comparative example 1 and the model group. Comparative examples 2, 3, and 4 had certain cell migration ability, but none of them was as good as that of example 3, indicating that the combination of the three active substances played a synergistic role.

[0179] Example 6

[0180] Sun protection effect:

[0181] Samples: Example 3, Comparative Example 1.

[0182] This test uses human immortalized epidermal cells (HaCaT cells) as model cells to measure the effect of the samples on the vitality of skin cells damaged by UVB.

[0183] HaCaT cells were cultured at a rate of 1.0×10 5 Each well of the plate was inoculated with 100 μL of cells / mL at a density of 100 μL. After culturing for 24 h in complete medium, the medium was removed and the plate was washed three times with PBS.

[0184] Then 100 μL of PBS buffer was added to each well, and the sample (20 mg / cm 2 ) was evenly spread on the lid of the 96-well plate, the blank group was covered with tin foil, and then irradiated with UVB (308nm) for 15min, with a total energy of 120mJ / cm 2 ;

[0185] The supernatant was removed and replaced with serum-free DMEM medium, and the culture was continued for 24 h;

[0186] After washing three times with PBS, 100 μL of 0.5 mg / mL MTT solution (prepared in DMEM) was added to each well. After being placed in an incubator for 4 hours, the solution was aspirated and 100 μL DMSO was added, and the absorbance was measured at 490 nm.

[0187] Cell viability was calculated according to the following formula:

[0188] Cell viability (%) = As / Ac×100%, where As is the absorbance of the test sample and Ac is the absorbance of the blank group.

[0189] Results Figure 4 , indicating that Example 3 has significant differences compared with the model group, and has the function of preventing UVB to protect keratinocytes. However, the cell viability of the sample of Comparative Example 1 showed no significant difference from that of the model group.

[0190] It can be seen from the moisturizing effect and barrier repair effect tests, anti-aging experiments and sunscreen tests that the sunscreen revitalizing lip balm of the present invention has multiple functions, and has moisturizing, nourishing, repairing and anti-inflammatory soothing effects that are significantly superior to commercially available products, and has anti-aging and sunscreen effects. The active ingredients therein are naturally red, and the red effect can be achieved without the need for pigments. It is a composite multi-functional sunscreen revitalizing lip balm.

[0191] The present invention invents a lip care sunscreen multi-chamber microcapsule, which is compounded with three effective ingredients: prodigiosin, astaxanthin, and pyrroloquinoline quinone, and has the effects of anti-aging and sunscreen. The present invention adopts nanotechnology and low-temperature spray freezing technology to prepare multi-chamber microcapsules, which can realize enzyme-controlled release, greatly improve the transdermal effect of active substances, and improve their bioavailability.

[0192] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the present invention.

Claims

1. A lip care sunscreen multi-chamber microcapsule, characterized in that: Includes, prodigiosin, astaxanthin, pyrroloquinoline quinone, candelilla wax, shea butter, lauryl laurate, zein, hydrolyzed corn starch octenyl succinate, silica, and benzyl ether myristate; Calculated by the mass percentage of the raw materials, the prodigiosin is 0.1% to 5%, the astaxanthin is 0.1% to 5%, the pyrroloquinoline quinone is 0.1% to 5%, the candelilla wax is 5% to 20%, the shea butter is 5% to 20%, the lauryl laurate is 5% to 10%, the zein is 1% to 5%, the hydrolyzed corn starch octenyl succinate is 20% to 40%, the silica is 10% to 20%, and the benzyl ether myristate is 5% to 10%.

2. The lip care sunscreen multi-chamber microcapsule according to claim 1, characterized in that: The multi-chamber microcapsule achieves enzyme-controlled release.

3. The method for preparing the lip care sunscreen multi-chamber microcapsules according to claim 1 or 2, characterized in that: include, Heat prodigiosin, astaxanthin, pyrroloquinoline quinone, candelilla wax and shea butter until they are completely miscible to form phase A; Heat lauryl laurate, benzyl ether myristate and water until they are completely miscible to form phase B; Slowly add phase A into phase B at 80°C and stir evenly, then homogenize under high pressure at 800 bar to obtain phase C; Zein and hydrolyzed corn starch octenyl succinate were dissolved in deionized water and hydrated overnight to obtain a transparent liquid, which was then heated in a water bath at 90° C. with stirring, and silica was added and stirred to obtain phase D; Mix phase C and phase D, stir evenly, put them into a micro high-pressure atomizing pump, use an open container to hold liquid nitrogen, then aim the atomizing pump nozzle at the liquid nitrogen and start the pump to spray; After spraying, the open container is placed in a freeze dryer and vacuum dried to obtain multi-chamber microcapsules.

4. The preparation method according to claim 3, characterized in that: The heating temperature during the preparation of phase A is 80°C.

5. The preparation method according to claim 3, characterized in that: The heating temperature during the preparation of phase B is 80°C.

6. The preparation method according to claim 5, characterized in that: The number of high pressure homogenization times in the process of preparing phase C is 3 to 5 times.

7. The preparation method according to claim 6, characterized in that: The nozzle of the spray is 0.2 mm.

8. Use of the lip care sunscreen multi-chamber microcapsules according to claim 1 or 2 in the preparation of sunscreen revitalizing lip balm.

9. The use according to claim 8, characterized in that: The sunscreen activating lip balm comprises the following components in mass fractions: Lip care sunscreen multi-chamber microcapsule 10%~30%, microcrystalline wax 10%~30%, mica 10%~15%, walnut seed oil 2%~10%, isononyl isononanoate 2%~10%, jojoba seed oil 2%~10%, polyglyceryl-3-diisostearate 2%~5%, hydroxypropyl tetrahydropyrantriol 0.1%~2%, sodium hyaluronate 0.1%~2%, glucomannan 0.1%~2%, water is added to 100%.

10. The use according to claim 9, characterized in that: The preparation method of the sunscreen activating lip balm comprises: Mixing microcrystalline wax, mica, walnut seed oil, isononyl isononanoate, jojoba seed oil, and polyglycerol-3-diisostearate, stirring, dissolving, heating, and stirring until uniformly dissolved, to prepare a first phase; Hydroxypropyl tetrahydropyrantriol, sodium hyaluronate, glucomannan and water are mixed, stirred, dissolved, heated and stirred until uniformly dissolved to prepare a second phase; Mix the first phase and the second phase and stir evenly, add the lip care sunscreen multi-chamber microcapsules, keep warm and stir to disperse evenly, cool the paste to 62°C, pour it into a mold or barrel to form and discharge, and prepare a sunscreen revitalizing lip balm.

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