Traditional Chinese medicine composition for treating xerophthalmia, fumigating and washing agent and preparation method and application of fumigating and washing agent

Abstract

The invention belongs to the field of traditional Chinese medicine, and relates to a traditional Chinese medicine composition for treating xerophthalmia, a fuming and washing agent and a preparation method and application thereof, and the traditional Chinese medicine composition for treating xerophthalmia comprises the following components in parts by weight: 20-30 parts of chrysanthemum extract dry powder, 15-20 parts of wolfberry extract dry powder, 12-18 parts of radix ophiopogonis extract dry powder and 5-10 parts of radix polygonati officinalis extract dry powder. The traditional Chinese medicine composition is prepared from 10-20 parts of radix angelicae sinensis extract dry powder, 5-15 parts of radix astragali extract dry powder, 8-14 parts of radix salviae miltiorrhizae extract dry powder, 11-17 parts of radix ampelopsis extract dry powder and 10-15 parts of sedum sarmentosum extract dry powder. The invention provides a traditional Chinese medicine composition containing chrysanthemum extract dry powder, wolfberry extract dry powder, radix ophiopogonis extract dry powder, polygonatum odoratum extract dry powder, angelica sinensis extract dry powder, astragalus membranaceus extract dry powder, salvia miltiorrhiza extract dry powder, ampelopsis japonica extract dry powder and sedum sarmentosum extract dry powder. The total effective rate of the traditional Chinese medicine composition for treating xerophthalmia can reach 90% or above.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition for treating dry eye, a fumigation and washing agent, and a preparation method and application thereof. Background Art

[0002] Dry eye, also known as conjunctivitis sicca, is a common eye disease characterized by insufficient tear secretion, excessive evaporation, or abnormal tear composition. Symptoms of dry eye include pain, foreign body sensation, blurred vision, dryness, etc. In severe cases, eye burning, soreness, redness, eye pain, photophobia, etc. may occur, and may develop into corneal damage. Therefore, timely treatment is required to avoid the development of corneal ulcers, perforations, or secondary infections. With the changes in lifestyles in modern society, factors such as long-term use of electronic screens, air pollution, and air-conditioned environments have exacerbated the incidence of dry eye.

[0003] At present, the treatments for dry eye mainly include artificial tear eye drops, oral medications, and physical therapy, etc. Among them, artificial tears are the most commonly used method to relieve symptoms, but long-term use of artificial tears may cause a certain burden on the cornea, oral medications may have side effects, and the disadvantage of physical therapy is that it is inconvenient to operate.

[0004] Therefore, the existing treatment methods for dry eye have limited effects and may cause allergic reactions or other adverse reactions. Therefore, it is necessary to provide a product that can effectively treat dry eye and reduce side effects. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In order to solve the problems of poor therapeutic effect and side effects of products for treating dry eyes in the prior art, the present invention provides a traditional Chinese medicine composition for treating dry eyes, a fumigation and washing agent, and a preparation method and application thereof.

[0007] (II) Technical solution

[0008] In order to achieve the above object, the main technical solutions adopted by the present invention include:

[0009] In a first aspect, the present invention provides a traditional Chinese medicine composition for treating dry eye syndrome, comprising, by weight, 20-30 parts of chrysanthemum extract dry powder, 15-20 parts of wolfberry extract dry powder, 12-18 parts of ophiopogon extract dry powder, 5-10 parts of polygonatum odoratum extract dry powder, 10-20 parts of angelica extract dry powder, 5-15 parts of astragalus extract dry powder, 8-14 parts of salvia miltiorrhiza extract dry powder, 11-17 parts of white peony root extract dry powder, and 10-15 parts of scutellaria baicalensis extract dry powder.

[0010] The Chinese medicine composition for treating dry eye as described above preferably comprises, by weight, 24-26 parts of chrysanthemum extract dry powder, 16-19 parts of wolfberry extract dry powder, 14-16 parts of ophiopogon extract dry powder, 6-9 parts of polygonatum odoratum extract dry powder, 12-16 parts of angelica extract dry powder, 8-12 parts of astragalus extract dry powder, 9-13 parts of salvia miltiorrhiza extract dry powder, 12-16 parts of white peony root extract dry powder, and 12-14 parts of scutellaria baicalensis extract dry powder.

[0011] The Chinese medicine composition for treating dry eye as described above, preferably, comprises, by weight, 25 parts of chrysanthemum extract dry powder, 18 parts of wolfberry extract dry powder, 15 parts of ophiopogon extract dry powder, 8 parts of polygonatum odoratum extract dry powder, 15 parts of angelica extract dry powder, 10 parts of astragalus extract dry powder, 11 parts of salvia miltiorrhiza extract dry powder, 14 parts of white peony extract dry powder, and 13 parts of scutellaria baicalensis extract dry powder.

[0012] In a second aspect, the present invention provides a method for preparing the above-mentioned Chinese medicine composition, comprising the following steps:

[0013] A1: prepare dry powders of chrysanthemum extract, wolfberry extract, ophiopogon extract, polygonatum odoratum extract, angelica extract, astragalus extract, salvia miltiorrhiza extract, white peony extract and scutellaria baicalensis extract respectively;

[0014] A2: Mix the various extract powders prepared in step A1 to obtain a Chinese medicine composition.

[0015] In the method for preparing the traditional Chinese medicine composition as described above, preferably, the various extract powders in step A1 are prepared by the following method:

[0016] A11: Grind chrysanthemum, wolfberry, ophiopogon, polygonatum, angelica, astragalus, salvia miltiorrhiza, white ampel and scutellaria baicalensis respectively to obtain different medicinal powders;

[0017] A12: The above-mentioned various medicinal powders are subjected to a wall-breaking treatment, and then water or ethanol is added to the various medicinal materials after the wall-breaking treatment, respectively, for pressure extraction treatment, followed by filtering to obtain different medicinal solutions;

[0018] A13: The above-mentioned various medicinal liquids are respectively subjected to reduced pressure concentration treatment to obtain different concentrated solutions, and then the above-mentioned various concentrated solutions are respectively spray-dried and then pulverized to obtain chrysanthemum extract dry powder, wolfberry extract dry powder, ophiopogon extract dry powder, polygonatum odoratum extract dry powder, angelica extract dry powder, astragalus extract dry powder, salvia miltiorrhiza extract dry powder, white peony extract dry powder and scutellaria baicalensis extract dry powder, respectively.

[0019] In the method for preparing the Chinese medicine composition as described above, preferably, in step A11, chrysanthemum, wolfberry, ophiopogon, polygonatum, angelica, astragalus, salvia miltiorrhiza, white ampel and scutellaria baicalensis are respectively crushed into 80-120 meshes;

[0020] In step A12, the volume of water or ethanol solution is 4-8 times the volume of the powder, the volume concentration of ethanol used is 50-95%, the pressure of the pressurized extraction treatment is 0.3-0.5 MPa, and the time of the pressurized extraction treatment is 2-4 hours.

[0021] In a third aspect, the present invention provides a fumigant for treating dry eye, comprising the above-mentioned Chinese medicine composition.

[0022] In a fourth aspect, the present invention provides a method for preparing the above-mentioned fumigant for treating dry eye, comprising the following steps:

[0023] S1: uniformly mix 20-30 parts of chrysanthemum extract dry powder, 15-20 parts of wolfberry extract dry powder, 12-18 parts of ophiopogon extract dry powder, 5-10 parts of polygonatum odoratum extract dry powder, 10-20 parts of angelica extract dry powder, 5-15 parts of astragalus extract dry powder, 8-14 parts of salvia miltiorrhiza extract dry powder, 11-17 parts of white smilax glabra extract dry powder and 10-15 parts of scutellaria baicalensis extract dry powder to obtain a traditional Chinese medicine composition;

[0024] S2: sterilizing the Chinese medicine composition, then adding sterile water and heating it to obtain a fumigant.

[0025] In the method for preparing the fumigant for treating dry eye as described above, preferably, in step S2, the mass ratio of the traditional Chinese medicine composition to sterile water is 1:20-1:50.

[0026] In a fifth aspect, the present invention further provides a use of the above-mentioned Chinese medicine composition or the Chinese medicine composition prepared by the above-mentioned preparation method in eye patches, eye ointments and nebulizer liquids.

[0027] (III) Beneficial effects

[0028] The present invention provides a Chinese medicine composition comprising dry powder of chrysanthemum extract, dry powder of wolfberry extract, dry powder of ophiopogon extract, dry powder of polygonatum odoratum extract, dry powder of angelica extract, dry powder of astragalus extract, dry powder of salvia miltiorrhiza extract, dry powder of white smilax glabra extract and dry powder of scutellaria baicalensis extract, which has significant curative effect in treating dry eye. According to clinical trial statistics, the total effective rate of the Chinese medicine composition in treating dry eye can reach more than 90%. The dosage of the Chinese medicine composition of the present invention is scientific and reasonable, the adverse reaction is low, and the recurrence rate of treating dry eye with the Chinese medicine composition of the present invention can also be significantly reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1This is a statistical chart of the average tear secretion of mice in the blank control group, negative control group, and experimental groups 1-8 after 14 days of administration;

[0030] Figure 2 Statistical graph of corneal staining scores of mice in the blank control group, negative control group, and experimental groups 1-8 after 14 days of administration. DETAILED DESCRIPTION

[0031] In order to better explain the present invention and facilitate understanding, the present invention is described in detail below in conjunction with specific implementation methods.

[0032] The invention provides a traditional Chinese medicine composition for treating dry eye syndrome. The traditional Chinese medicine composition comprises, by weight, 20-30 parts of chrysanthemum extract dry powder, 15-20 parts of wolfberry extract dry powder, 12-18 parts of ophiopogon extract dry powder, 5-10 parts of polygonatum odoratum extract dry powder, 10-20 parts of angelica extract dry powder, 5-15 parts of astragalus extract dry powder, 8-14 parts of salvia miltiorrhiza extract dry powder, 11-17 parts of white peony root extract dry powder and 10-15 parts of scutellaria baicalensis extract dry powder.

[0033] Preferably, the traditional Chinese medicine composition of the present invention comprises, by weight, 24-26 parts of chrysanthemum extract dry powder, 16-19 parts of wolfberry extract dry powder, 14-16 parts of ophiopogon extract dry powder, 6-9 parts of polygonatum odoratum extract dry powder, 12-16 parts of angelica extract dry powder, 8-12 parts of astragalus extract dry powder, 9-13 parts of salvia miltiorrhiza extract dry powder, 12-16 parts of white ampel extract dry powder, and 12-14 parts of scutellaria baicalensis extract dry powder.

[0034] Further preferably, the Chinese medicine composition of the present invention comprises, by weight, 25 parts of chrysanthemum extract dry powder, 18 parts of wolfberry extract dry powder, 15 parts of ophiopogon extract dry powder, 8 parts of polygonatum odoratum extract dry powder, 15 parts of angelica extract dry powder, 10 parts of astragalus extract dry powder, 11 parts of salvia miltiorrhiza extract dry powder, 14 parts of white ampel extract dry powder, and 13 parts of scutellaria baicalensis extract dry powder.

[0035] The main causes of dry eye include insufficient tear secretion, excessive evaporation or abnormal tear composition. The therapeutic principle of the Chinese medicine composition of the present invention for dry eye includes clearing the liver and improving eyesight, nourishing yin and promoting fluid production, invigorating qi and replenishing blood, nourishing blood and promoting blood circulation, and clearing heat and removing dampness. Specifically, the chrysanthemum extract dry powder and wolfberry extract dry powder in the Chinese medicine composition of the present invention mainly have the effect of clearing the liver and improving eyesight, the ophiopogon extract dry powder and the polygonatum odoratum extract dry powder have the main effect of nourishing yin and promoting fluid production, which can replenish the body's yin fluid, increase tear secretion, and relieve dry eyes, the angelica extract dry powder and the astragalus extract dry powder have the main effect of invigorating qi and replenishing blood, which can improve the whole body's qi and blood conditions, thereby nourishing the eyes, the salvia miltiorrhiza extract dry powder has the main effect of nourishing blood and promoting blood circulation, which can improve the blood circulation of the eyes, enhance the nutritional supply of the eyes, and reduce eye fatigue, and the white smilax extract dry powder and the scutellaria baicalensis extract dry powder have the main effect of clearing heat and removing dampness, which can remove damp heat in the body, reduce inflammatory reactions, and improve the eye environment. In addition, the combination of chrysanthemum and wolfberry also has significant neurotrophic activity.

[0036] The above are the main effects of each component. The detailed pharmacology of each raw material component is as follows:

[0037] Chrysanthemum: It is pungent, sweet, bitter, slightly cold, and enters the lung and liver meridians. It has the effects of dispersing wind-heat, clearing away heat and detoxifying, calming the liver and improving eyesight. Modern pharmacological studies have shown that chrysanthemum has the function of protecting the liver, exhibiting strong antioxidant activity, and can inhibit inflammatory reactions and alleviate inflammatory symptoms. The vitamin A and beta-carotene contained in chrysanthemum have a positive effect on protecting eyesight and improving eyesight.

[0038] Lycium barbarum: sweet and neutral in nature, it enters the liver and kidney meridians. Lycium barbarum has the effects of nourishing the liver and kidney, improving eyesight and strengthening bones and muscles. Modern pharmacological research shows that Lycium barbarum is rich in antioxidants, such as β-carotene, vitamin C, vitamin E, etc. These ingredients can resist free radical damage, play an anti-inflammatory, protect nerves, protect the liver and eyes, and are very beneficial to the health of the liver and eyes.

[0039] Ophiopogon japonicus: sweet, slightly bitter, cold, and enters the heart, lung, and stomach meridians. Ophiopogon japonicus has the effects of nourishing yin and moistening the lungs, benefiting the stomach and promoting fluid production, and clearing the heart and relieving restlessness. Modern pharmacological studies have shown that Ophiopogon japonicus exerts anti-inflammatory effects by controlling the production of pro-inflammatory factors such as NO and TNF-α. The main active ingredient of Ophiopogon japonicus - steroidal saponin compounds, has the characteristics of scavenging free radicals in the body, anti-oxidation, and has a protective effect on the liver.

[0040] Polygonatum odoratum: sweet in nature and slightly cold, it enters the lung and stomach meridians. Polygonatum odoratum has the effects of promoting fluid production and moistening the lungs, nourishing yin and nourishing the stomach, and tonifying the kidneys. Its effect is mild and does not nourish greasy and restrain evil. Modern pharmacological studies have shown that Polygonatum odoratum has significant antioxidant activity, and its main material basis is Polygonatum odoratum polysaccharide and Polygonatum odoratum flavonoids. The active ingredients Polygonatum odoratum polysaccharide and saponin in Polygonatum odoratum can enhance the body's immune function and protect liver cells from damage.

[0041] Angelica: Sweet, pungent, warm in nature, it enters the liver, heart, and spleen meridians. Angelica has the effects of nourishing blood, activating blood circulation, regulating menstruation and relieving pain, and moistening the intestines and relieving constipation. Modern pharmacological studies have shown that Angelica has the effects of enhancing and regulating immune function, anti-oxidation and anti-inflammatory effects, can promote blood circulation, improve blood fluidity, and also has a certain antibacterial effect.

[0042] Astragalus: sweet in nature, slightly warm, and enters the lung and spleen meridians. Astragalus has the effects of replenishing qi and raising yang, strengthening the exterior and stopping sweating, promoting diuresis and reducing swelling, promoting fluid and nourishing blood, expelling toxins and draining pus, and healing sores and promoting tissue regeneration. Modern pharmacological studies have shown that Astragalus has the effects of enhancing immunity, anti-oxidation, and relieving fatigue.

[0043] Danshen: bitter in nature and slightly cold, it enters the heart and liver meridians. Danshen has the effects of promoting blood circulation and removing blood stasis, clearing the heart and relieving restlessness, and calming the mind. Modern pharmacology has found that Danshen has a certain antibacterial effect, can reduce inflammation, and has the ability to scavenge free radicals.

[0044] Bletilla striata: bitter in nature and slightly cold, it enters the heart and stomach meridians. Bletilla striata has the effects of clearing away heat and detoxifying, eliminating carbuncle and dispersing knots, and healing sores and promoting tissue regeneration. Modern pharmacological studies have shown that Bletilla striata has the effect of regulating immune activity, protecting liver cells, promoting liver cell regeneration, and improving liver function.

[0045] Creeping pot grass: It is sweet, light and cool in nature, and has the effects of clearing away heat and dampness, detoxifying and reducing swelling. Modern pharmacological studies have shown that the flavonoids in Creeping pot grass are its main chemical components, which can significantly reduce the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the body. It is its main active ingredient for lowering enzymes, protecting the liver, repairing damaged liver cells and anti-inflammatory, and has a significant protective effect on the liver.

[0046] The present invention also provides a method for preparing the above-mentioned Chinese medicine composition, comprising the following steps:

[0047] A1: Prepare chrysanthemum extract dry powder, wolfberry extract dry powder, ophiopogon extract dry powder, polygonatum odoratum extract dry powder, angelica extract dry powder, astragalus extract dry powder, salvia miltiorrhiza extract dry powder, white peony extract dry powder and scutellaria baicalensis extract dry powder respectively.

[0048] A2: Mix the various extract powders prepared in step A1 according to 20-30 parts of chrysanthemum extract powder, 15-20 parts of wolfberry extract powder, 12-18 parts of ophiopogon extract powder, 5-10 parts of polygonatum odoratum extract powder, 10-20 parts of angelica extract powder, 5-15 parts of astragalus extract powder, 8-14 parts of salvia miltiorrhiza extract powder, 11-17 parts of white peony root extract powder, and 10-15 parts of scutellaria baicalensis extract powder to obtain a traditional Chinese medicine composition.

[0049] The preparation methods of the above-mentioned various Chinese medicine extract dry powders are as follows:

[0050] A11: Grind chrysanthemum, wolfberry, ophiopogon, polygonatum, angelica, astragalus, salvia miltiorrhiza, white ampel and creeping spatholobi into 80-120 meshes respectively to obtain different medicinal powders.

[0051] A12: The above-mentioned various medicinal powders are subjected to a wall-breaking treatment, and then water or ethanol is added to the various medicinal materials after the wall-breaking treatment, and a pressure extraction treatment is performed, followed by filtration to obtain different medicinal liquids. Preferably, the volume of the water or ethanol solution in this step is 4-8 times the volume of the medicinal powder, the volume concentration of the ethanol used is 50-95%, the pressure of the pressure extraction treatment is 0.3-0.5MPa, and the time of the pressure extraction treatment is 2-4h. Further preferably, the number of extractions of the pressure extraction can be 2-3 times, and the temperature of the pressure extraction can be 50-70°C.

[0052] A13: The above-mentioned various medicinal liquids are respectively subjected to reduced pressure concentration treatment to obtain different concentrated solutions, and then the above-mentioned various concentrated solutions are respectively spray-dried at a temperature of 50-60°C, and then pulverized to obtain chrysanthemum extract dry powder, wolfberry extract dry powder, ophiopogon extract dry powder, polygonatum odoratum extract dry powder, angelica extract dry powder, astragalus extract dry powder, salvia miltiorrhiza extract dry powder, white peony extract dry powder and scutellaria baicalensis extract dry powder, respectively.

[0053] The present invention also provides a fumigant containing the Chinese medicine composition, and the fumigant can also be used for treating dry eyes.

[0054] The fumigation agent can avoid the disadvantage of Chinese medicine being too bitter to swallow, and can directly act on the eyes. At the same time, fumigation itself can promote the efficacy of the medicine and increase blood circulation. The fumigation agent of the present invention is used to treat dry eye, and the recurrence rate after the cure is stopped is lower than that of antibiotics, tear gland secretion promoting drugs and the like for treating dry eye in the prior art.

[0055] Specifically, the preparation method of the above-mentioned fumigation agent for treating dry eyes comprises the following steps:

[0056] S1: Evenly mix 20-30 parts of chrysanthemum extract dry powder, 15-20 parts of wolfberry extract dry powder, 12-18 parts of ophiopogon extract dry powder, 5-10 parts of polygonatum odoratum extract dry powder, 10-20 parts of angelica extract dry powder, 5-15 parts of astragalus extract dry powder, 8-14 parts of salvia miltiorrhiza extract dry powder, 11-17 parts of white peony root extract dry powder and 10-15 parts of scutellaria baicalensis extract dry powder to obtain a traditional Chinese medicine composition.

[0057] S2: sterilizing the Chinese medicine composition, specifically by gamma ray sterilization or electron beam sterilization, and then adding sterile water so that the mass ratio of the Chinese medicine composition to the sterile water is 1:20-1:50, and then heating the mixed solution to 40-45° C. to obtain a fumigant.

[0058] In addition, in addition to the fumigant, the Chinese medicine composition of the present invention can also be used as the main component of eye patches, atomizing liquids, eye ointments, etc.

[0059] In order to further clarify the scheme of the present invention and its technical advancement, the following is a description in conjunction with specific embodiments and technical effects.

[0060] Example 1

[0061] This embodiment provides a fumigation agent for treating dry eye, comprising a Chinese medicine composition and sterile water. The Chinese medicine composition comprises, by weight, 25 parts of chrysanthemum extract powder, 18 parts of wolfberry extract powder, 15 parts of ophiopogon extract powder, 8 parts of polygonatum odoratum extract powder, 15 parts of angelica extract powder, 10 parts of astragalus extract powder, 11 parts of salvia miltiorrhiza extract powder, 14 parts of white peony extract powder, and 13 parts of scutellaria baicalensis extract powder.

[0062] The preparation method of the fumigant is as follows:

[0063] S1: Weigh the dry powder of the extracts of the above components, mix them evenly, and obtain a Chinese medicine composition.

[0064] S2: sterilizing the Chinese medicine composition with gamma rays, then adding sterile water, the mass ratio of the Chinese medicine composition to the sterile water being 1:40, and then heating the mixed solution to 42° C. to obtain a fumigant.

[0065] Example 2

[0066] This embodiment provides a fumigation agent for treating dry eye, comprising a Chinese medicine composition and sterile water. The Chinese medicine composition comprises, by weight, 30 parts of chrysanthemum extract powder, 20 parts of wolfberry extract powder, 18 parts of ophiopogon extract powder, 10 parts of polygonatum odoratum extract powder, 20 parts of angelica extract powder, 15 parts of astragalus extract powder, 14 parts of salvia miltiorrhiza extract powder, 17 parts of white peony extract powder, and 15 parts of scutellaria baicalensis extract powder.

[0067] The preparation method of the fumigant is as follows:

[0068] S1: Weigh the dry powder of the extracts of the above components, mix them evenly, and obtain a Chinese medicine composition.

[0069] S2: The Chinese medicine composition is subjected to electron beam sterilization treatment, and then sterile water is added, the mass ratio of the Chinese medicine composition to the sterile water is 1:50, and then the mixed solution is heated to 45° C. to obtain a fumigant.

[0070] Example 3

[0071] This embodiment provides a fumigation agent for treating dry eye, comprising a Chinese medicine composition and sterile water. The Chinese medicine composition comprises, by weight, 24 parts of chrysanthemum extract powder, 16 parts of wolfberry extract powder, 14 parts of ophiopogon extract powder, 6 parts of polygonatum odoratum extract powder, 12 parts of angelica extract powder, 8 parts of astragalus extract powder, 9 parts of salvia miltiorrhiza extract powder, 12 parts of white peony extract powder, and 12 parts of scutellaria baicalensis extract powder.

[0072] The preparation method of the fumigant is as follows:

[0073] S1: Weigh the dry powder of the extracts of the above components, mix them evenly, and obtain a Chinese medicine composition.

[0074] S2: The Chinese medicine composition is subjected to electron beam sterilization treatment, and then sterile water is added, the mass ratio of the Chinese medicine composition to the sterile water is 1:30, and then the mixed solution is heated to 41° C. to obtain a fumigant.

[0075] Example 4

[0076] This embodiment provides a fumigation agent for treating dry eye, comprising a Chinese medicine composition and sterile water. The Chinese medicine composition comprises, by weight, 26 parts of chrysanthemum extract powder, 19 parts of wolfberry extract powder, 16 parts of ophiopogon extract powder, 9 parts of polygonatum odoratum extract powder, 16 parts of angelica extract powder, 12 parts of astragalus extract powder, 13 parts of salvia miltiorrhiza extract powder, 16 parts of white peony extract powder, and 14 parts of scutellaria baicalensis extract powder.

[0077] The preparation method of the fumigant is as follows:

[0078] S1: Weigh the dry powder of the extracts of the above components, mix them evenly, and obtain a Chinese medicine composition.

[0079] S2: sterilizing the Chinese medicine composition with gamma rays, then adding sterile water, the mass ratio of the Chinese medicine composition to the sterile water being 1:35, and then heating the mixed solution to 43° C. to obtain a fumigant.

[0080] Example 5

[0081] This embodiment provides a fumigation agent for treating dry eye, comprising a Chinese medicine composition and sterile water. The Chinese medicine composition comprises, by weight, 20 parts of chrysanthemum extract powder, 15 parts of wolfberry extract powder, 12 parts of ophiopogon extract powder, 5 parts of polygonatum odoratum extract powder, 10 parts of angelica extract powder, 5 parts of astragalus extract powder, 8 parts of salvia miltiorrhiza extract powder, 11 parts of white peony root extract powder, and 10 parts of scutellaria baicalensis extract powder.

[0082] The preparation method of the fumigant is as follows:

[0083] S1: Weigh the dry powder of the extracts of the above components, mix them evenly, and obtain a Chinese medicine composition.

[0084] S2: The Chinese medicine composition is subjected to electron beam sterilization treatment, and then sterile water is added, the mass ratio of the Chinese medicine composition to the sterile water is 1:20, and then the mixed solution is heated to 40° C. to obtain a fumigant.

[0085] Comparative Example 1

[0086] This comparative example provides a fumigation wash for treating dry eye, which is different from Example 1 in that the amount of dry powder of Bletilla striata extract in the traditional Chinese medicine composition is 0.

[0087] Comparative Example 2

[0088] This comparative example provides a fumigation wash for treating dry eyes, which is different from Example 1 in that the amount of dry powder of the Herba Crepisae extract in the Chinese medicine composition is 0.

[0089] Comparative Example 3

[0090] This comparative example provides a fumigation wash for treating dry eye, which is different from Example 1 in that the proportions of dry powder of Bletilla striata extract and dry powder of Creeping Jasmine extract in the traditional Chinese medicine composition are both 0.

[0091] The effects of the Chinese medicine compositions prepared in Examples 1-5 and Comparative Examples 1-3 on dry eye in mice were investigated, and the specific contents are as follows:

[0092] Healthy 8-week-old BALB / c mice were selected, half male and half female. Before the experiment, mice with abnormal corneal fluorescence staining and large difference in tear secretion between the two eyes were eliminated by measuring corneal fluorescence staining scores and tear secretion volume.

[0093] The mice were evenly divided into blank control group, negative control group, experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6, experimental group 7 and experimental group 8. There were 8 mice in each group, and the number of eyes in each group was 16. The average value of the number of eyes was used for calculating the corneal staining score and tear secretion volume.

[0094] The mice in the blank control group were raised normally without any treatment. The mice in the negative control group and experimental groups 1-8 were instilled with 1 mg / ml benzalkonium chloride solution 3 times a day for 14 consecutive days, and the eyelids of the mice were closed for about 90 seconds after each instillation.

[0095] After the dry eye model was established, the eye drops prepared from the Chinese medicine compositions prepared in Examples 1-5 and Comparative Examples 1-3 were respectively dripped into both eyes of the mice in Experimental Groups 1 to 8, 5 times a day for 14 consecutive days. An equal volume of normal saline was dripped into both eyes of the mice in the blank control group and the negative control group every day.

[0096] The phenol red cotton thread experiment was used to measure the average tear secretion of the mice in the above groups after successful modeling, as well as the tear secretion after 14 days of administration. Specifically, the phenol red cotton thread was placed on the conjunctiva of the lower eyelid of the mouse at one-third of the distance from the outer corner of the eye, and was taken out after 60 seconds to measure the wet length of the phenol red cotton thread (mm). The test results are shown in Table 1 (average tear secretion after modeling, unit: mm) and Figure 1 (Average tear secretion volume after 14 days of administration, unit: mm, where AJ groups correspond to blank control group, negative control group, experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6, experimental group 7 and experimental group 8, respectively). The tear secretion volume in Table 1 is the mean ± standard deviation.

[0097] Table 1 Statistics of the average tear secretion of mice in the blank control group, negative control group and experimental groups 1-8

[0098]

[0099]

[0100] Figure 1 In the table, the same letters (abc) indicate that there is no statistical difference between the corresponding groups (P>0.05), while different letters indicate that there is a statistical difference between the corresponding groups (P<0.05).

[0101] Table 1 and Figure 1 It can be seen that the mice in the blank control group were not modeled or medicated, and the average tear secretion of the mice was always maintained in the normal range. After modeling, the average tear secretion of mice in the negative control group and experimental groups 1 to 8 was significantly reduced. Under the condition of only dripping normal saline, the average tear secretion of mice in the negative control group did not improve. After 14 days of administration, the average tear secretion of mice in experimental groups 1 to 5 returned to normal levels, greater than 5mm / 1min. The average tear secretion of mice in experimental groups 6-8 was improved, but still did not reach 5mm / 1min. Among them, the increase in the average tear secretion of mice in experimental groups 6 and 7 was slightly higher than the increase in the average tear secretion of mice in experimental group 8.

[0102] After the dry eye model was established (day 0 after successful modeling) and 14 days after administration, the blank control mice, the negative control group and the mice in experimental groups 1-8 were stained with 0.25wt% sodium fluorescein solution, and the 0.25wt% sodium fluorescein solution was allowed to stay on the ocular surface of each group of mice for 10s, and then the excess dye was rinsed with 0.9wt% sodium chloride solution, and the ocular surface was wiped dry with a cotton swab. The eyeballs of each group of mice were exposed under a slit lamp for observation, and photos were taken after magnification to record the coloring of the sodium fluorescein dye on the cornea of ​​each group of mice.

[0103] The mouse cornea was divided into four quadrants: superior temporal, inferior temporal, superior nasal, and inferior nasal. Each quadrant was scored from 0 to 3 points. The scoring criteria were: no staining was 0 points; 1 to 30 punctate staining (mild) was 1 point; more than 30 punctate staining but the staining was not fused (moderate) was 2 points; corneal punctate staining fusion, filaments, and ulcers (severe) was 3 points. Each corneal score was the sum of the scores of the four quadrants. The scoring results are shown in Table 2 (Corneal staining scores after the dry eye model was established) and Figure 2 (Corneal staining scores after 14 days of administration, where AJ groups correspond to blank control group, negative control group, experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6, experimental group 7 and experimental group 8, respectively). The corneal staining scores in Table 2 are mean values ​​± standard deviations.

[0104] Table 2 Statistics of corneal staining scores in the blank group, negative control group, and experimental groups 1-8

[0105]

[0106] In Table 2, the rules for corneal staining scoring are as follows: no staining is 0 points, 1-30 punctate staining (mild) is 1 point, more than 30 punctate staining but staining is not fused (moderate) is 2 points, corneal punctate staining fusion, filaments and ulcers (severe) is 3 points. Specifically, the lower the corneal score, the higher the degree of corneal epithelial repair of the mouse and the better the eye recovery, and the higher the score, the more severe the corneal damage. Figure 2 In the table, the same letters (abc) indicate that there is no statistical difference between the corresponding groups (P>0.05), while different letters indicate that there is a statistical difference between the corresponding groups (P<0.05).

[0107] Table 2 and Figure 2 It can be seen that the mice in the blank control group did not undergo modeling and medication, and the cornea remained at a normal level. After modeling, the corneal epithelial staining area of ​​the mice in the negative control group and experimental groups 1 to 8 increased significantly. After 14 days of medication, the corneal epithelial staining area of ​​the mice in experimental groups 1 to 5 was significantly reduced, and the degree of recovery was high. The degree of repair of the corneal epithelial staining area of ​​the mice in experimental groups 6-8 was significantly lower than that in experimental groups 1-5.

[0108] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine composition for treating dry eye, characterized in that: Calculated by weight, it includes 20-30 parts of chrysanthemum extract dry powder, 15-20 parts of wolfberry extract dry powder, 12-18 parts of ophiopogon extract dry powder, 5-10 parts of polygonatum extract dry powder, 10-20 parts of angelica extract dry powder, 5-15 parts of astragalus extract dry powder, 8-14 parts of salvia miltiorrhiza extract dry powder, 11-17 parts of white peony extract dry powder, and 10-15 parts of scutellaria baicalensis extract dry powder.

2. The Chinese medicine composition for treating dry eye according to claim 1, characterized in that: Calculated by weight, it includes 24-26 parts of chrysanthemum extract dry powder, 16-19 parts of wolfberry extract dry powder, 14-16 parts of ophiopogon extract dry powder, 6-9 parts of polygonatum odoratum extract dry powder, 12-16 parts of angelica extract dry powder, 8-12 parts of astragalus extract dry powder, 9-13 parts of salvia miltiorrhiza extract dry powder, 12-16 parts of white peony extract dry powder, and 12-14 parts of scutellaria baicalensis extract dry powder.

3. The Chinese medicine composition for treating dry eye according to claim 1, characterized in that: Calculated by weight, it includes 25 parts of chrysanthemum extract dry powder, 18 parts of wolfberry extract dry powder, 15 parts of ophiopogon extract dry powder, 8 parts of polygonatum odoratum extract dry powder, 15 parts of angelica extract dry powder, 10 parts of astragalus extract dry powder, 11 parts of salvia miltiorrhiza extract dry powder, 14 parts of white peony extract dry powder, and 13 parts of scutellaria baicalensis extract dry powder.

4. A method for preparing the Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The steps include: A1: prepare dry powders of chrysanthemum extract, wolfberry extract, ophiopogon extract, polygonatum odoratum extract, angelica extract, astragalus extract, salvia miltiorrhiza extract, white peony extract and scutellaria baicalensis extract respectively; A2: Mix the various extract powders prepared in step A1 to obtain a Chinese medicine composition.

5. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The various extract powders in step A1 are prepared by the following method: A11: Grind chrysanthemum, wolfberry, ophiopogon, polygonatum, angelica, astragalus, salvia miltiorrhiza, white ampel and scutellaria baicalensis respectively to obtain different medicinal powders; A12: The above-mentioned various medicinal powders are subjected to a wall-breaking treatment, and then water or ethanol is added to the various medicinal materials after the wall-breaking treatment, respectively, for pressure extraction treatment, followed by filtering to obtain different medicinal solutions; A13: The above-mentioned various medicinal liquids are respectively subjected to reduced pressure concentration treatment to obtain different concentrated solutions, and then the above-mentioned various concentrated solutions are respectively spray-dried and then pulverized to obtain chrysanthemum extract dry powder, wolfberry extract dry powder, ophiopogon extract dry powder, polygonatum odoratum extract dry powder, angelica extract dry powder, astragalus extract dry powder, salvia miltiorrhiza extract dry powder, white peony extract dry powder and scutellaria baicalensis extract dry powder, respectively.

6. The method for preparing the Chinese medicine composition according to claim 5, characterized in that: In step A11, chrysanthemum, wolfberry, ophiopogon, polygonatum, angelica, astragalus, salvia miltiorrhiza, white ampel and scutellaria baicalensis are respectively crushed into 80-120 meshes; In step A12, the volume of water or ethanol solution is 4-8 times the volume of the powder, the volume concentration of ethanol used is 50-95%, the pressure of the pressurized extraction treatment is 0.3-0.5 MPa, and the time of the pressurized extraction treatment is 2-4 hours.

7. A fumigant for treating dry eyes, characterized in that: A Chinese medicine composition comprising any one of claims 1 to 3.

8. A method for preparing the fumigant for treating dry eye according to claim 7, characterized in that: The steps include: S1: uniformly mix 20-30 parts of chrysanthemum extract dry powder, 15-20 parts of wolfberry extract dry powder, 12-18 parts of ophiopogon extract dry powder, 5-10 parts of polygonatum odoratum extract dry powder, 10-20 parts of angelica extract dry powder, 5-15 parts of astragalus extract dry powder, 8-14 parts of salvia miltiorrhiza extract dry powder, 11-17 parts of white smilax glabra extract dry powder and 10-15 parts of scutellaria baicalensis extract dry powder to obtain a traditional Chinese medicine composition; S2: sterilizing the Chinese medicine composition, then adding sterile water and heating it to obtain a fumigant.

9. The method for preparing the fumigant for treating dry eye according to claim 8, characterized in that: In step S2, the mass ratio of the Chinese medicine composition to sterile water is 1:20-1:

50.

10. Use of the Chinese medicine composition according to any one of claims 1 to 3 or the Chinese medicine composition prepared by the preparation method according to any one of claims 4 to 6 in eye patches, eye ointments and atomized liquids.

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