Rhodomyrtus tomentosa essential oil and application of compound essential oil based on rhodomyrtus tomentosa essential oil in pest control

Abstract

The invention belongs to the technical field of biological control of pests, and particularly relates to rhodomyrtus tomentosa essential oil and application of compound essential oil based on the rhodomyrtus tomentosa essential oil in pest control. The compound essential oil comprises rhodomyrtus tomentosa essential oil, pepper essential oil, capsicum essential oil and lemon grass essential oil. The rhodomyrtus tomentosa leaf essential oil and the compound essential oil preparation thereof have poisoning and killing effects on various pests such as spodoptera frugiperda, solenopsis invicta, crape myrtle long-spot aphid and flat valley thief in agriculture and forestry, and show good poisoning, contact killing and fumigating activities. The invention can lay a foundation for the development of botanical biopesticides, and provides a new way for the popularization of a green pest prevention and control technology.

Description

Technical Field

[0001] The invention belongs to the technical field of biological pest control, and particularly relates to application of myrtle essential oil and a compound essential oil based thereon in controlling pests. Background Art

[0002] The fall armyworm (Spodopterafrugiperda), a Lepidoptera insect of the family Noctuidae, is a major agricultural pest on the global alert of the Food and Agriculture Organization of the United Nations. In 2017, the International Center for Agriculture and Biosciences listed it as one of the "Top Ten Plant Pests in the World".

[0003] The red fire ant (Solenopsis invicta) is an insect of the genus Solenopsis in the family Formicidae in the order Hymenoptera. The red fire ant originated in Brazil, Paraguay and the Paraná River City in Argentina. It was introduced to the southern United States in the 1930s and is currently the most dangerous ant in the world. The red fire ant is very aggressive. Children, the elderly and people with allergies are the "high-risk groups" threatened by the red fire ant. In the areas where the red fire ant invades, it often brings serious ecological disasters and is a major enemy of biodiversity protection and agricultural production. It invades houses, schools, lawns and other places, and has a greater chance of contact with people, and bites occur from time to time. The red fire ant chews on wires, often causing short circuits and even small fires.

[0004] The crape myrtle aphid (Tinocallis kahawaluokalani) belongs to the family Aphidoideae of the order Hemiptera. The crape myrtle aphid mainly harms the leaves of crape myrtle. The insects are swarming, and the back of the young leaves is often covered with pests. The leaves are often curled and uneven, and the new shoots of the affected plants are twisted, the development of flower buds is inhibited, and the inflorescence is shortened, affecting flowering. It can also induce sooty mold disease and spread viral diseases.

[0005] The long-horned flour beetle (Cryptolestespusillus) is an insect of the family Cryptolepidae in the order Coleoptera. It is distributed all over the world and is an important stored grain pest. It is a late-stage pest. Adults and larvae damage broken or damaged grains, oilseeds, flour, beans, dried fruits and other agricultural products and their processed products. Sometimes the insect population density is extremely high. In addition to directly feeding, it often causes agricultural products to heat up and mold.

[0006] At present, the control of pests mainly adopts chemical pesticides. However, the long-term use of chemical pesticides will inevitably lead to the development of pest resistance. At present, the fall armyworm has developed resistance to a variety of insecticides (carbaryl, methyl parathion, diazinon, fluvalinate, bifenthrin, tralomethrin, cypermethrin, methomyl, chlorpyrifos).

[0007] Since the 1960s until now, people have relied on the chemical agent phosphine to control the long-grained flat grain beetle.

[0008] However, the long-term, single, and large-scale use of phosphine has caused the long-horned flat grain beetle to develop serious resistance to pesticides. Rice stored for a year sometimes needs to be fumigated 3 to 4 times. The crape myrtle aphid reproduces quickly and has many generations. It is easy to develop resistance to pesticides. It has developed resistance to a variety of insecticides (acetamiprid water dispersible granules, imidacloprid wettable powder, emamectin benzoate, etc.). The effectiveness of red fire ant baits is greatly reduced when exposed to water. The excessive use of chemical pesticides will not only remain in the soil and water sources and continue to pollute the environment, but also destroy the ecosystem around the farmland, causing the soil to become barren and lose fertility, affecting the growth and yield of crops, and even causing harm to human health. Therefore, it is urgent to find green and sustainable pest control methods.

[0009] Plant essential oil is an important secondary metabolite existing in different tissues of plants. The main effects of plant essential oil on pests include repelling, attracting, and contact killing. A large number of studies have shown that plant essential oils have high biological activity against pests. The use of plant essential oil preparations to control pests is not only non-toxic and harmless to humans and the environment, but also because of its complex composition, it is not easy for pests to develop resistance. It is an environmentally friendly, safe, and effective agricultural control method. Myrtle (Rhodomyrtus tomoentosa), belonging to the Myrtaceae family, is an excellent wild medicinal plant resource, widely distributed in Southeast Asian countries and regions, especially in southern China, Japan, Thailand, and Vietnam. Myrtle leaf essential oil has antioxidant, anti-inflammatory, and antibacterial activities.

[0010] Currently, the application of myrtle extract in insecticide is relatively scarce. Summary of the invention

[0011] In view of the deficiencies in the prior art, the present invention provides an application of myrtle essential oil and a compound essential oil based thereon in preventing and controlling pests, so as to enhance the insecticidal effect of plant essential oil preparations.

[0012] In order to achieve the above object, the present invention is implemented by the following scheme:

[0013] The invention provides a myrtle essential oil, which is composed of the following effective ingredients in parts by weight:

[0014] 0.58 parts of 2,4-dimethyl-3-pentanone, 0.67 parts of n-hexanal, 0.95 parts of hexamethylcyclotrisiloxane, 0.99 parts of trans-2-hexenal, 0.95 parts of cis-4-hexen-1-ol, 0.67 parts of hexyl formate, 0.43 parts of heptaldehyde, 0.62 parts of 1,5-pentanediol, 1.52 parts of 2-methyl-4-heptanone, 0.51 parts of (Z)-2-heptenal, 2.77 parts of benzaldehyde, 0.68 parts of n-heptanol, 0.38 parts of 1-octen-3-ol, 0.31 parts of methyl heptenone, 1.31 parts of tris(trimethylsiloxy)silane, 0.5 parts of (E)-3-hexen-1-ol acetate, 0.45 parts of isopropyl isocyanate, 0.99 parts of eucalyptol, 0.26 parts of 2,2,6-trimethylcyclohexanone, 0.26 parts of trans-2-dodecenal, 1.23 parts of 3-methyltetrahydrophthalic anhydride, 8.95 parts of octanol, 0.22 parts of trans-linalool oxide (furans), 0.22 parts of 4-isopropenyltoluene, 0.47 parts of hexamethylcyclotrisiloxane, 7.63 parts of linalool, 4.48 parts of fenchol, 0.15 parts of succinic acid, tridec-2-yn-1-yl pentafluorophenyl ester, 0.9 5 parts, (-)-trans-pine carveol 3.5 parts, camphene hydrate 0.34 parts, N-[(Pentafluorophenyl)methylene]-β,3,4-tris(trimethylsilyloxy)benzeneethanamine 0.8 parts, 3-nolanthone 1.37 parts, 2-camphenol 2.95 parts, nonane-1-ol 0.6 parts, 4-terpineol 5.23 parts, L-α-terpineol 34.05 parts, myrtenol 0.96 parts, dodecane 0.23 parts, terpineol 0.18 parts of Luozhou camphor, 0.36 parts of (-)-verbenone, 0.33 parts of 3,4-dimethylbenzaldehyde, 0.64 parts of (1R,5S)-rel-carveol, 0.13 parts of (S)-(+)-carvone, 0.07 parts of trans-2-caren-4-ol, 0.34 parts of dodecamethylcyclohexasiloxane, 0.23 parts of benzyl 2-methylbenzoate, 0.36 parts of benzyl valerate, 0.4 parts of tetrakis(trimethylsiloxy)silane, 0.08 parts of 2,4-di-tert-butylphenol, 14-hydroxy-alpha-muurole ne 0.14 parts, dodecamethylcyclohexasiloxane 0.12 parts, alpha-calacorene 0.08 parts, but-3-yn-2-ylpentyl fumarate 0.1 parts, maaliol 0.13 parts, caryophyllene alcohol 0.1 parts, eucalyptol 0.8 parts, virgin olive oil 0.26 parts, bicyclo [6.3.0] undec-1 (8) -en-3-one, 2,2,5,5-tetramethylol 0.23 parts, Ylangenol 1.45 parts, 1,3-dimethyl-7-pentyl-3,7-dihydro-1H-purine-2,6-dione 0.09 parts, T-ylangol 0.12 parts, aromatic tree ene-4,10-diol 0.84 parts, N-[(pentafluorophenyl)methylene]-β,3,4-tris(trimethylsilyloxy)benzeneethanamine 0.56 parts, (-)-isolongitol methyl ether 0.17 parts, D:A-Friedooleanan-2-one 0.03 parts, 10-methyl-2,5:3,10-dioxybicyclo[4.3.1]decane 0.11 parts, 5-methylsalicylic acid 0.11 parts, octadecylcyclononasiloxane 0.28 parts, dodecamethyldihydrohexasiloxane 0.2 parts, hexadecanoic acid ethyl ester 0.23 parts, 1,1,1,5,7,7,7-HEPTAMETHYL-3,3-BIS 0.61 parts. .

[0015] The invention also provides application of the myrtle essential oil in preventing and controlling pests.

[0016] The invention provides a compound essential oil, which is composed of the following effective ingredients: the myrtle essential oil, pepper essential oil, chili essential oil and lemongrass essential oil according to claim 1.

[0017] In the present invention, the concentration of the myrtle essential oil is 20-40 ml / L, the concentration of the pepper essential oil is 3-30 ml / L, the concentration of the chili essential oil is 10-30 ml / L, and the concentration of the lemongrass essential oil is 5-10 ml / L.

[0018] The invention also provides application of the compound essential oil in preventing and controlling pests.

[0019] The present invention also provides application of the compound essential oil in preventing and controlling red fire ants.

[0020] The present invention also provides application of the compound essential oil in preventing and controlling fall armyworm.

[0021] The invention also provides application of the compound essential oil in preventing and controlling crape myrtle aphids.

[0022] The present invention also provides application of the compound essential oil in preventing and treating long-horned flat grain beetle.

[0023] The present invention uses fall armyworm, red imported fire ant, crape myrtle aphid, long-horned flat grain beetle as test insects, and uses myrtle and its compound essential oil to carry out insecticidal activity determination, and finds that various combinations of essential oil mixtures have certain toxicity to the test insects. Therefore, the present invention further provides an effective combination concentration range of an essential oil mixture with toxicity to multiple pests, and the essential oil mixture has the following beneficial effects compared with the prior art.

[0024] The invention discloses a myrtle leaf essential oil component and the preparation and application of its compound essential oil. The myrtle leaf essential oil is prepared by distillation, and other various plant essential oils are provided by Ji'an Huashuo Spice Oil Co., Ltd. A large number of biological assays show that the mixed poison has a strong insecticidal effect on fall armyworm, red fire ant, crape myrtle aphid, and long-horned flat grain beetle, and can be used to prepare an environmentally friendly essential oil insecticide to prevent and control the above pests. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is the chromatogram of the volatiles of essential oil from Myrtle leaves detected by gas chromatography-mass spectrometry (GC-MS);

[0026] Figure 2 A is the control group for the biological activity test on red fire ants. Figure 2 B is the experimental group for the biological activity determination experiment on red imported fire ants;

[0027] Figure 3 A is the control group of red fire ants, Figure 3 B is the experimental group of red imported fire ants;

[0028] Figure 4 A is a diagram of the experimental process of fall armyworm. Figure 4 B is the dead fall armyworm, Figure 4 C is the surviving fall armyworm;

[0029] Figure 5 It is the experimental material of the crape myrtle aphid;

[0030] Figure 6 A is the control group of crape myrtle aphids, Figure 6 B is the experimental group of crape myrtle aphid;

[0031] Figure 7 A is the control group of crape myrtle aphids. Figure 7 B is the experimental group of crape myrtle aphids;

[0032] Figure 8 A and B are the experimental processes of the long-horned flat grain beetle;

[0033] Fig. 9 A is the control group of long-horned flat beetles, Fig. 9 B is the experimental group of the long-horned flat grain beetle. DETAILED DESCRIPTION

[0034] The present invention is further described in detail below in conjunction with the accompanying drawings and specific examples of the specification. The examples are only used to explain the present invention and are not used to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0035] Example 1

[0036] Myrtle Leaf Essential Oil Extract

[0037] Myrtle leaves are picked in Zhanjiang, Guangdong. The healthy and pest-free complete leaves are placed in a distiller for direct distillation to obtain myrtle leaf essential oil.

[0038] The myrtle leaf essential oil obtained above has a unique aromatic odor. After detection by gas chromatography-mass spectrometry, its composition and content are as follows:

[0039] Table 1 Components and percentages of essential oil from myrtle leaves

[0040]

[0041]

[0042]

[0043] Example 2

[0044] Preparation of myrtle compound essential oil and its insecticidal effect on red imported fire ants

[0045] 1. Experimental Methods

[0046] 1. Sources

[0047] Red fire ants are captured from the wild and cultured in the laboratory.

[0048] Myrtle leaf essential oil was prepared by the distillation method described in Example 1, and pepper essential oil, chili essential oil, and lemongrass essential oil were purchased from Ji'an Huashuo Spice Oil Co., Ltd.

[0049] The red fire ant anti-escape liquid is a mixture of talcum powder and anhydrous ethanol in a ratio of 1:1.

[0050] 2. Preparation of compound essential oils

[0051] Step 1: Preparation of solvent

[0052] Mix Tween 80 and water in a ratio of 1:2000 in a 65° water bath and shake well to obtain the solvent.

[0053] Step 2: Preparation of emulsifier

[0054] Mix Nongru 600# and OP-10 at a concentration of 1.15% and a volume ratio of 1:1, and shake well to obtain an emulsifier.

[0055] Step 3: Preparation of essential oil compound

[0056] According to the compound components shown in Table 2, the compound essential oil mixture of each formula is prepared with the emulsifier provided in step 2, and then the above mixture is fully emulsified by an ultrasonic oscillator to obtain the compound essential oil of different formulas. At the same time, a blank control and a Myrtle single essential oil control (30 ml / L) are set.

[0057] Table 2 Components of the myrtle compound essential oil for red fire ants

[0058]

[0059] 3. Test on contact killing activity of compound essential oil against red fire ants

[0060] The red fire ants were used as test insects. The red fire ants were placed in a glass wide-mouth bottle (the bottle mouth was treated to prevent escape), 30 heads / bottle. The above formulas 1, 2, 3, 4 and the control were placed in a spray bottle and sprayed evenly on the body surface of the red fire ants in an appropriate amount. Each treatment was repeated 3 times, 30 heads / repetition. The treated red fire ants were placed in a constant temperature incubator at 26°C, and the death was observed and recorded after 24 hours.

[0061] 4. Test on the toxicity of compound essential oil to red imported fire ants

[0062] The sliced ​​ham sausage was immersed in the above-mentioned compound 1, 2, 5, 6 and control solution for 3 seconds, then taken out and dried until there was no obvious moisture, and then placed in a wide-mouth bottle containing red fire ants for feeding red fire ants. Each treatment was repeated 3 times, 30 heads / repetition. The treated red fire ants were placed in a constant temperature incubator at 26℃, and the death was observed and recorded after 24 hours.

[0063] 2. Experimental Results

[0064]

[0065] The contact killing effect of the compound essential oil on red fire ants is shown in Table 3.

[0066] Table 3 Mortality statistics of the red fire ant contact test group

[0067]

[0068]

[0069] The poisoning effect of the compound essential oil on red fire ants is shown in Table 4.

[0070] Table 4 Mortality statistics of the red fire ant poison feeding experimental group

[0071] deal with Experimental Group 1 Experimental Group 2 Experimental Group 3 average value Blank control 0％ 0％ 0％ 0％ Single essential oil 40％ 66.67％ 66.67％ 57.78％ Compound 1 90％ 100％ 100％ 96.67％ Compound 2 100％ 100％ 100％ 100％ Compound 5 100％ 100％ 96.67％ 98.89％ Compound 6 100％ 100％ 93.33％ 97.78％

[0072] The results of the analysis show that the compound essential oil has a synergistic effect on the myrtle leaf essential oil in controlling red fire ants, with both contact activity and feeding activity. The above formulas all have good insecticidal effects, and the antennae of dead red fire ants curl up (attached Figure 3 -B). For this purpose, the concentration of myrtle essential oil in the compound essential oil is in the range of 20-40 ml / L, the concentration of pepper essential oil is in the range of 3-30 ml / L, the concentration of chili essential oil is in the range of 10-30 ml / L, and the concentration of lemongrass essential oil is in the range of 5-10 ml / L.

[0073] Example 3

[0074] Preparation of compound essential oil and its insecticidal effect on fall armyworm

[0075] 1. Experimental Methods

[0076] 1. Sources

[0077] The fall armyworm is collected from the wild and cultivated in the laboratory.

[0078] The remaining experimental materials are the same as in Example 2.

[0079] 2. Preparation of compound essential oils

[0080] According to the compound components shown in Table 5, the compound essential oil mixtures of various formulas were prepared with the emulsifier prepared in step 2 of Example 2, and then the above mixtures were fully emulsified using an ultrasonic oscillator to obtain compound essential oils of different formulas. At the same time, a blank control and a Myrtle essential oil control (30 ml / L) were set.

[0081] Table 5 Components of the myrtle compound essential oil against fall armyworm

[0082]

[0083] 3. Test on contact killing of compound essential oil against fall armyworm

[0084] The above compound essential oil, blank control example and single essential oil example were used as raw materials to conduct contact killing experiment on fall armyworm. The raw materials were placed in a spray bottle and sprayed evenly on the surface of fall armyworm in an appropriate amount. Observe under shade, take 10 heads in each group, repeat 3 groups, and place in a shaded and cool place. Observe the results after 24 hours and count the number of deaths.

[0085] 2. Experimental Results

[0086]

[0087] The contact killing effect of the compound essential oil on fall armyworm is shown in Table 6.

[0088] Table 6 Mortality statistics of the fall armyworm contact test group

[0089] deal with Experimental Group 1 Experimental Group 2 Experimental Group 3 average value Blank control 0％ 0％ 0％ 0％ Single essential oil 40％ 60％ 60％ 53.33％ Compound 1 80％ 100％ 100％ 93.33％ Compound 2 100％ 100％ 70％ 90％ Compound 3 100％ 100％ 90％ 96.67％ Compound 4 100％ 90％ 80％ 90％

[0090] The results of the analysis showed that both the myrtle leaf essential oil and the compound essential oil had a contact killing effect on the larvae of the fall armyworm, and the compound essential oil had a more obvious effect. Figure 4 -B), the color becomes darker and curled up. For this reason, the concentration of myrtle essential oil in the myrtle compound essential oil is in the range of 20-35 ml / L, the concentration of pepper essential oil is 3-10 ml / L, the concentration of chili essential oil is 10-30 ml / L, and the concentration of lemongrass essential oil is 5-10 ml / L.

[0091] Example 4

[0092] Preparation of compound essential oil and its insecticidal effect on crape myrtle aphid

[0093] 1. Experimental Methods

[0094] 1. Sources

[0095] The crape myrtle aphid was captured and cultured in the laboratory at Guangdong Ocean University.

[0096] The remaining experimental materials are the same as in Example 2.

[0097] 2. Preparation of compound essential oils

[0098] According to the compound components shown in Table 7, the compound essential oil mixtures of various formulas were prepared with the emulsifier prepared in step 2 of Example 2, and then the above mixtures were fully emulsified using an ultrasonic oscillator to obtain compound essential oils of different formulas. At the same time, a blank control and a Myrtle essential oil control (30 ml / L) were set.

[0099] Table 7 Components of the myrtle compound essential oil against crape myrtle aphids

[0100]

[0101] 3. Toxicity test of compound essential oil on crape myrtle aphid

[0102] The above formulas 1, 3, 4, 5 and blank control examples and single essential oil examples were used as raw materials. Thirty crape myrtle aphids were placed on leaves in each group, and the raw materials were dipped with cotton and placed on the petiole for absorption by the leaves. Repeat 3 groups. The experimental materials were placed in a petri dish and observed in a dark place. The results were checked after 24 hours, and the number of deaths was counted.

[0103] 4. Test on contact killing of compound essential oil against crape myrtle aphid

[0104] The above formulas 1, 2, 4, 5 and blank control examples and single essential oil examples were used as raw materials. Thirty crape myrtle aphids were placed on leaves in each group, and cotton dipped in clean water was placed on the petiole to provide moisture for the leaves. Repeat 3 groups. The raw materials were placed in a spray bottle and sprayed evenly on the surface of the crape myrtle aphid in an appropriate amount. The experimental materials were placed in a petri dish and observed in a dark place. The results were checked after 24 hours.

[0105] 2. Experimental Results

[0106]

[0107] The effect of compound essential oil on crape myrtle aphids is shown in Table 8.

[0108] Table 8 Mortality statistics of the crape myrtle aphid feeding poison experimental group

[0109] deal with Experimental Group 1 Experimental Group 2 Experimental Group 3 average value Blank control 0％ 0％ 0％ 0％ Single essential oil 60％ 60％ 70％ 63.33％ Compound 1 100％ 100％ 100％ 100％ Compound 3 100％ 100％ 100％ 100％ Compound 4 90％ 90％ 93.33％ 91.11％ Compound 5 100％ 100％ 100％ 100％

[0110] The contact killing effect of the compound essential oil on the crape myrtle aphid is shown in Table 9.

[0111] Table 9 Mortality statistics of the contact-killing experimental group of Lagerstroemia indica

[0112] deal with Experimental Group 1 Experimental Group 2 Experimental Group 3 average value Blank control 0％ 0％ 0％ 0％ Single essential oil 60％ 70％ 70％ 66.67％ Compound 1 100％ 100％ 100％ 100％ Compound 2 100％ 100％ 100％ 100％ Compound 4 100％ 100％ 100％ 100％ Compound 5 100％ 100％ 100％ 100％

[0113] The results of the analysis showed that the compound essential oil had a synergistic effect on the myrtle leaf essential oil in controlling the crape myrtle aphid. The contact killing effect of each compound essential oil reached 100%, and all had good insecticidal effects. The dead crape myrtle aphid turned darker, and its legs were curled up and deformed (see attached Figure 7 -B). It can be seen that in the compound essential oil preparation for Lagerstroemia indica, the concentration of myrtle essential oil is 25-40 ml / L, the concentration of pepper essential oil is 3-10 ml / L, the concentration of chili essential oil is 10-30 ml / L, and the concentration of lemongrass essential oil is 5-10 ml / L.

[0114] Example 5

[0115] Preparation of compound essential oil and its insecticidal effect on long-horned castanea

[0116] 1. Experimental Methods

[0117] 1. Sources

[0118] The long-horned flat grain beetle originated from the long-term cultivation in the laboratory of Guangdong Ocean University.

[0119] The remaining experimental materials are the same as in Example 2.

[0120] 2. Preparation of compound essential oils

[0121] According to the compound components shown in Table 10, the compound essential oil mixtures of various formulas were prepared with the emulsifier prepared in step 2 of Example 2, and then the mixtures were fully emulsified using an ultrasonic oscillator to obtain compound essential oils of different formulas. At the same time, a blank control and a Myrtle essential oil control (30 ml / L) were set.

[0122] Table 10 Components of the myrtle compound essential oil against long-horned flat grain beetles

[0123]

[0124] 3. Fumigation test of compound essential oil on long-horned castanea

[0125] The above-mentioned compound recipes 1, 2, 3, 4, blank control examples, and single essential oil examples were used as raw materials. 20 long-horned flat grain beetles were placed in a glass tube with a stopper for light shielding and observation. The same paper strips were cut and dipped into 30uL of each formula essential oil solution and stuffed next to the lid. Repeat for 3 groups. Check the results after 24 hours and count the number of deaths.

[0126] 2. Experimental Results

[0127]

[0128] The fumigation effect of the compound essential oil on long-horned flat grain beetle is shown in Table 11.

[0129] Table 11 Mortality statistics of the long-horned flat grain beetle fumigation experimental group

[0130] deal with Experimental Group 1 Experimental Group 2 Experimental Group 3 average value Blank control 0％ 0％ 0％ 0％ Single essential oil 60％ 60％ 70％ 63.33％ Compound 1 100％ 100％ 100％ 100％ Compound 2 100％ 100％ 100％ 100％ Compound 3 100％ 100％ 100％ 100％ Compound 4 100％ 100％ 100％ 100％

[0131] From the above fumigation data, the compound essential oil also has a significant improvement effect on the myrtle leaf essential oil in the prevention and treatment of long-horned flat grain beetles. The fumigation killing effect of each compound essential oil reached 100%, and the dead long-horned flat grain beetles were curled up and reduced in size (see attached Fig. 9 -B). In short, the essential oil compound for long-horned castanea includes 20-40 ml / L myrtle essential oil, 3-10 ml / L pepper essential oil, 10-30 ml / L chili essential oil and 5-10 ml / L lemongrass essential oil.

[0132] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. For ordinary technicians in this field, other different forms of changes or modifications can be made based on the above descriptions and ideas. It is not necessary and impossible to list all the implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A myrtle essential oil, characterized in that The myrtle essential oil is composed of the following active ingredients in parts by weight: 0.58 parts of 2,4-dimethyl-3-pentanone, 0.67 parts of n-hexanal, 0.95 parts of hexamethylcyclotrisiloxane, 0.99 parts of trans-2-hexenal, 0.95 parts of cis-4-hexen-1-ol, 0.67 parts of hexyl formate, 0.43 parts of heptaldehyde, 0.62 parts of 1,5-pentanediol, 1.52 parts of 2-methyl-4-heptanone, 0.51 parts of (Z)-2-heptenal, 2.77 parts of benzaldehyde, 0.68 parts of n-heptanol, 0.38 parts of 1-octen-3-ol, 0.31 parts of methyl heptenone, 1.31 parts of tris(trimethylsiloxy)silane, 0.5 parts of (E)-3-hexen-1-ol acetate, 0.45 parts of isopropyl isocyanate, 0.99 parts of eucalyptol, 0.26 parts of 2,2,6-trimethylcyclohexanone, 0.26 parts of trans-2-dodecenal, 1.23 parts of 3-methyltetrahydrophthalic anhydride, 8.95 parts of octanol, 0.22 parts of trans-linalool oxide (furans), 0.22 parts of 4-isopropenyltoluene, 0.47 parts of hexamethylcyclotrisiloxane, 7.63 parts of linalool, 4.48 parts of fenchol, 0.15 parts of succinic acid, tridec-2-yn-1-yl pentafluorophenyl ester, 0.9 5 parts, (-)-trans-pine carveol 3.5 parts, camphene hydrate 0.34 parts, N-[(Pentafluorophenyl)methylene]-β,3,4-tris(trimethylsilyloxy)benzeneethanamine 0.8 parts, 3-nolanthone 1.37 parts, 2-camphenol 2.95 parts, nonane-1-ol 0.6 parts, 4-terpineol 5.23 parts, L-α-terpineol 34.05 parts, myrtenol 0.96 parts, dodecane 0.23 parts, terpineol 0.18 parts of Luozhou camphor, 0.36 parts of (-)-verbenone, 0.33 parts of 3,4-dimethylbenzaldehyde, 0.64 parts of (1R,5S)-rel-carveol, 0.13 parts of (S)-(+)-carvone, 0.07 parts of trans-2-caren-4-ol, 0.34 parts of dodecamethylcyclohexasiloxane, 0.23 parts of benzyl 2-methylbenzoate, 0.36 parts of benzyl valerate, 0.4 parts of tetrakis(trimethylsiloxy)silane, 0.08 parts of 2,4-di-tert-butylphenol, 14-hydroxy-alpha-muurole ne 0.14 parts, dodecamethylcyclohexasiloxane 0.12 parts, alpha-calacorene 0.08 parts, but-3-yn-2-ylpentyl fumarate 0.1 parts, maaliol 0.13 parts, caryophyllene alcohol 0.1 parts, eucalyptol 0.8 parts, virgin olive oil 0.26 parts, bicyclo [6.3.0] undec-1 (8) -en-3-one, 2,2,5,5-tetramethylol 0.23 parts, Ylangenol 1.45 parts, 1,3-dimethyl-7-pentyl-3,7-dihydro-1H-purine-2,6-dione 0.09 parts, T-ylangol 0.12 parts, aromatic tree ene-4,10-diol 0.84 parts, N-[(pentafluorophenyl)methylene]-β,3,4-tris(trimethylsilyloxy)benzeneethanamine 0.56 parts, (-)-isolongitol methyl ether 0.17 parts, D:A-Friedooleanan-2-one 0.03 parts, 10-methyl-2,5:3,10-dioxybicyclo[4.3.1]decane 0.11 parts, 5-methylsalicylic acid 0.11 parts, octadecylcyclononasiloxane 0.28 parts, dodecamethyldihydrohexasiloxane 0.2 parts, hexadecanoic acid ethyl ester 0.23 parts, 1,1,1,5,7,7,7-HEPTAMETHYL-3,3-BIS 0.61 parts. .

2. Application of Myrtle Essential Oil according to claim 1 in pest control.

3. A compound essential oil, characterized in that: The compound essential oil consists of the following effective ingredients: the myrtle essential oil, pepper essential oil, chili essential oil and lemongrass essential oil described in claim 1.

4. The compound essential oil according to claim 3, characterized in that The concentration of the myrtle essential oil is 20-40 ml / L, the concentration of the pepper essential oil is 3-30 ml / L, the concentration of the chili essential oil is 10-30 ml / L, and the concentration of the lemongrass essential oil is 5-10 ml / L.

5. Use of the compound essential oil according to claim 3 or 4 in controlling pests.

6. Use of the compound essential oil according to claim 3 or 4 in preventing and controlling red imported fire ants.

7. Use of the compound essential oil according to claim 3 or 4 in preventing and controlling fall armyworm.

8. Use of the compound essential oil according to claim 3 or 4 in preventing and controlling Lagerstroemia indica aphids.

9. Use of the compound essential oil according to claim 3 or 4 in preventing and treating the long-horned flat grain beetle.

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