Device for metasurface fluid detection and use method

Abstract

The invention discloses a device for detecting metasurface fluid and a using method, and belongs to the field of microfluidics. The device comprises two acrylic plates, quartz, a fixing screw, an L-shaped needle tube, a Teflon gasket and a metasurface. Four corners of each acrylic plate are provided with internal thread grooves, the center of one acrylic plate is provided with a square groove, and the center of the square groove is provided with a hollow circle; a mushroom-shaped hollow is formed in the center of the top plate; the number of the fixing screws is four. The number of the L-shaped needle tubes is two. Two circular small holes are formed in the centers of the two sides of the quartz plate, so that a liquid sample can be conveniently injected and discharged; the two L-shaped needle tubes are connected with the acrylic plate top plate to control the injection of a fluid sample and the discharge of waste liquid; the quartz plate and the metasurface are attached to the square groove of the bottom plate of the acrylic plate through sizes; and the quartz plate, the metasurface and the Teflon gasket are clamped by the two acrylic plates through fixing screws. According to the invention, invasion and damage to the sample can be reduced, and efficient, accurate and low-cost metasurface fluid detection is realized.

Description

Technical Field

[0001] The invention relates to a device for super-surface fluid detection and a use method thereof, and belongs to the field of microfluidics. Background Art

[0002] In the field of biochemical detection, the detection of fluid samples is one of the core links. The rapid and accurate detection of biological molecules such as pathogens, proteins, nucleic acids, etc. is of great significance for disease diagnosis, drug development, food safety, etc. Traditional biochemical detection methods, such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), although they have high detection sensitivity and specificity, often have problems such as complex operation, long detection cycle, and high cost. To this end, we must continue to explore new detection technologies to meet the urgent needs of the field of biochemical detection for high throughput, high sensitivity, and rapid response. The ideal fluid detection technology should minimize the intrusion and destruction of the sample as much as possible to maintain the original state of the sample. The present invention aims to innovatively design an advanced fluid detection device in response to the above technical challenges and actual needs.

[0003] As a new type of artificial electromagnetic material, metasurface has unique physical properties and the ability to regulate electromagnetic waves. It can realize flexible and effective regulation of electromagnetic wave polarization, amplitude, phase, polarization mode, propagation mode and other characteristics. When electromagnetic waves pass through a fluid, their propagation characteristics will be affected by parameters such as the fluid's dielectric constant, viscosity, and density. By analyzing the transmission, reflection, and refraction characteristics of electromagnetic waves, relevant information about the fluid can be obtained. Metasurface technology uses this principle to achieve real-time monitoring of fluid parameters. The electromagnetic unit of the metasurface can achieve local resonance, so that the electromagnetic wave undergoes strong resonant absorption at a specific frequency. This local resonance effect helps to improve the sensitivity of fluid detection. By changing the geometric parameters or material properties of the metasurface electromagnetic unit, the operating frequency, detection range, sensitivity and other parameters of the fluid detection device can be regulated to meet the needs of different application scenarios. Summary of the invention

[0004] In order to solve the problems of high sample requirements, complex detection process, and destructiveness to samples in traditional fluid detection devices, the purpose of the present invention is to provide a device and method for use for supersurface fluid detection, which can achieve efficient, accurate and low-cost supersurface fluid detection, and is particularly suitable for supersurface fluid detection in the field of biochemical detection.

[0005] The purpose of the present invention is achieved through the following technical solutions.

[0006] The present invention discloses a device for super-surface fluid detection, comprising an acrylic plate, quartz, fixing screws, an L-shaped needle tube, a Teflon gasket and a super-surface. The acrylic plates are two, each of which has internal thread grooves at four corners, one of which is used as a bottom plate and has a square groove in the center, and a hollow circle in the center of the square groove; the other is used as a top plate and has a "mushroom-shaped" hollow in the center; the fixing screws are four; the L-shaped needle tubes are two; and the quartz plate has two circular holes in the center on both sides to facilitate the injection and outflow of liquid samples.

[0007] The two L-shaped needle tubes are connected to the top plate of the acrylic plate to control the injection of fluid samples and the discharge of waste liquid; the quartz plate and the super surface are fitted with the square groove of the bottom plate of the acrylic plate in size; the two acrylic plates clamp the quartz plate, the super surface and the Teflon gasket by fixing screws.

[0008] The present invention discloses a method for using a super-surface fluid detection device, which is implemented based on a device for super-surface fluid detection. The method for using a super-surface fluid detection device comprises the following steps:

[0009] Step 1: Place the acrylic sheet base flat on the table.

[0010] Step 2: Place the metasurface in the square groove of the acrylic base plate according to the polarization angle to be tested, with the coated side of the metasurface facing up.

[0011] Step 3: Place a Teflon gasket on the metasurface, wherein the structure of the metasurface is located in the middle of the Teflon gasket.

[0012] Step 4: Place the quartz sheet on the Teflon gasket. At this time, the quartz sheet and the super surface are precisely docked and fit into the square groove of the acrylic plate bottom plate.

[0013] Step 5: Cover the acrylic plate top plate with two L-shaped needle tubes and tighten the fixing screws. At this time, the two inlets and outlets of the L-shaped needle tubes need to be aligned with the two circular holes on the quartz plate to ensure the airtightness of the microcavity.

[0014] Step 6: Push the fluid sample to be tested into the device through one of the L-shaped needle tubes, and connect the end of the other L-shaped needle tube to the waste liquid collection container. After the fluid sample to be tested is evenly discharged, relevant tests are performed.

[0015] Beneficial effects:

[0016] 1. The present invention discloses a device for metasurface fluid detection and a method for using the device. By precisely assembling two acrylic plates, metamaterials and quartz sheets, a closed liquid microcavity system with a capacity of 13 μL is constructed, which can effectively achieve high-sensitivity trace detection of extremely low-volume samples such as sweat and blood; through two L-shaped needle tubes, the entry and discharge of fluid samples are controlled to reduce damage and contamination to the samples.

[0017] 2. The present invention discloses a device and method for super-surface fluid detection, which uses two acrylic plates and a quartz sheet, etc., and combines super-surface technology to form a relatively thin liquid layer. Compared with the traditional direct dripping method or the use of a thicker liquid layer, this design significantly improves the sensitivity of fluid detection and reduces the detection limit, thereby achieving more accurate micro-analysis.

[0018] 3. The present invention discloses a device for metasurface fluid detection and a method of use. The device has flexible disassembly and reassembly capabilities, and its metasurface components are designed to be replaceable so that they can be customized and optimized according to different experimental requirements, thereby adapting to diverse experimental content and research objectives.

[0019] 4. The present invention discloses a device for super-surface fluid detection and a method of use. After disassembly, the device can be cleaned by alcohol cleaning, ensuring the reusability of the device, thereby realizing the recycling and long-term effectiveness of the experimental device.

[0020] 5. The present invention discloses a device and method for detecting metasurface fluids. The device uses a combination of acrylic plates and fixing screws, which is convenient and time-saving. By directly integrating the metasurface with microfluidic technology, the traditional bonding step is omitted, which effectively reduces the cost of experiments and research and improves the economy of the overall experimental process. Low-cost metasurface fluid detection is particularly suitable for metasurface fluid detection in the field of biochemical detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the structure of a device for super-surface fluid detection according to an embodiment of the present invention.

[0022] Figure 2 This is a physical picture of the acrylic board bottom plate of an embodiment of the present invention.

[0023] Figure 3 This is a physical picture of a Teflon gasket according to an embodiment of the present invention.

[0024] Figure 4 This is a physical picture of a quartz plate according to an embodiment of the present invention.

[0025] Figure 5 This is a physical picture of the acrylic plate top plate of an embodiment of the present invention.

[0026] Figure 6 1. The alcohol fingerprint spectrum coupled sensing diagram (a) and line graph (b) of the embodiment of the present invention.

[0027] Figure 7 1. It is a comparative alcohol fingerprint spectrum coupled sensing diagram (a) and a line graph (b) of the present invention.

[0028] Among them, 1- acrylic plate bottom plate, 2- super surface, 3- Teflon gasket, 4- quartz plate, 5- L-type needle tube, 6- acrylic plate top plate, 7- fixing screws. DETAILED DESCRIPTION

[0029] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0030] like Figure 1 As shown, a device for super-surface fluid detection disclosed in this embodiment includes an acrylic plate 1, quartz, a fixing screw 7, an L-shaped needle tube 5, a Teflon gasket 3 and a super-surface 2; the acrylic plate 1 has two pieces, each of which has internal thread grooves (3mm in diameter) at four corners, and one piece is used as a bottom plate (60mm long, 50mm wide) with a square groove (30×30mm in diameter) at the center. 2 ), the center of the square groove has a hollow circle (diameter 26mm) Figure 2 Another piece is used as the top plate 6 (length 60mm, width 50mm) with a "mushroom-shaped" hollow opening in the center. Figure 5 As shown; the fixing screws 7 have four; the L-shaped needle tubes 5 have two; Figure 4 As shown, the quartz plate 4 (30×30×0.5mm 3 ) There are two circular holes in the center of the two sides to facilitate the injection and outflow of liquid samples;

[0031] The two L-shaped needle tubes 5 are connected to the top plate 6 of the acrylic plate 1 to control the injection of fluid samples and the discharge of waste liquid; the quartz plate 4 and the super surface 2 (30×30mm 2 , where the patterned part size is 10×10mm 2 ) The square groove of the bottom plate of the acrylic plate 1 is fitted in size; the two acrylic plates 1 are clamped with the quartz plate 4, the super surface 2 and the Teflon gasket 3 (inner diameter 22mm, outer diameter 28mm, thickness 0.025mm) by fixing screws 7;

[0032] This embodiment discloses a method for using a metasurface fluid detection device, and the specific implementation steps are as follows:

[0033] Step 1: Place the acrylic plate base 1 flat on the table.

[0034] Step 2: Place the metasurface 2 in the square groove of the acrylic base plate 1 according to the polarization angle to be tested, with the coated side of the metasurface facing upward.

[0035] Step 3: Place the Teflon gasket 3 on the metasurface 2 , wherein the structure of the metasurface 2 should be in the middle of the Teflon gasket 3 .

[0036] Step 4: Place the quartz plate 4 on the Teflon gasket 3. At this time, the quartz plate 4 and the metasurface 2 are precisely docked and perfectly fit into the square groove of the acrylic plate bottom plate 1.

[0037] Step 5: Cover the acrylic top plate 6 with two L-shaped needle tubes 5, and tighten the fixing screws 7. At this time, the two inlets and outlets of the L-shaped needle tube 5 need to be aligned with the two circular holes on the quartz plate 4 to ensure the airtightness of the microcavity.

[0038] Step 6: Push the fluid sample to be tested into the device through one of the L-shaped needle tubes, and connect the end of the other L-shaped needle tube to the waste liquid collection container. After the liquid is evenly discharged, the next related test can be carried out.

[0039] After installing the device according to the above steps, place it under the terahertz reflection lens for testing.

[0040] Test: Prepare alcohol solutions with concentrations of 0, 25%, 50%, 75%, and 100%, and perform fingerprint spectrum coupling tests in order from low to high concentrations. Perform linear coupling on the obtained reflectivity difference changes, such as Figure 6 shown.

[0041] Comparative Example

[0042] Test: Place the metasurface directly on an acrylic plate, use a pipette to drop the same volume of alcohol solution onto the metasurface in sequence, and perform fingerprint spectrum coupling tests in order from low to high concentrations. Perform linear coupling on the obtained reflectivity difference changes, such as Figure 7 shown.

[0043] The test results using the device and method of this embodiment are better than those using direct dripping, indicating the superiority and feasibility of the present invention.

[0044] The specific description above further illustrates the purpose, technical solutions and beneficial effects of the invention in detail. It should be understood that the above is only a specific embodiment of the present invention and is not intended to limit the scope of protection of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A device for super-surface fluid detection, characterized in that: The invention comprises an acrylic plate (1), quartz, a fixing screw (7), an L-shaped needle tube (5), a Teflon gasket (3) and a super surface (2); the acrylic plate (1) has two pieces, each of which has four corners with internal thread grooves, one of which is used as a bottom plate and has a square groove in the center, and a hollow circle in the center of the square groove (as shown in FIG2); the other is used as a top plate (6) and has a "mushroom-shaped" hollow in the center; the fixing screws (7) have four pieces; the L-shaped needle tubes (5) have two pieces; as shown in FIG4, two circular holes are opened in the center of both sides of the quartz plate (4) to facilitate the injection and outflow of liquid samples.

2. A device for super-surface fluid detection as claimed in claim 1, characterized in that: The two L-shaped needle tubes (5) are connected to the top plate (6) of the acrylic plate (1) to control the injection of fluid samples and the discharge of waste liquid; the quartz plate (4) and the super surface (2) are fitted with the square groove of the bottom plate of the acrylic plate (1) in size; the two acrylic plates (1) clamp the quartz plate (4), the super surface (2) and the Teflon gasket (3) by fixing screws (7).

3. A method for using a super-surface fluid detection device, based on the device for super-surface fluid detection according to claim 1 or 2, characterized in that: The following steps are included: Step 1: Place the acrylic plate base (1) flat on the table; Step 2: Place the metasurface (2) in the square groove of the acrylic base plate (1) according to the polarization angle to be tested, with the coated side of the metasurface (2) facing upwards; Step 3: placing a Teflon gasket (3) on the super surface (2), wherein the structure of the super surface (2) is located in the middle of the Teflon gasket (3); Step 4: Place the quartz plate (4) on the Teflon gasket (3). At this time, the quartz plate (4) and the super surface (2) are precisely docked and fit into the square groove of the acrylic plate bottom plate (1). Step 5: Cover the acrylic plate top plate (6) with two L-shaped needle tubes (5) and tighten the fixing screws (7); at this time, the two inlets and outlets of the L-shaped needle tubes (5) need to be aligned with the two circular holes on the quartz plate (4) to ensure the airtightness of the microcavity; Step 6: Push the fluid sample to be tested into the device through one of the L-shaped needle tubes, and connect the end of the other L-shaped needle tube to the waste liquid collection container; after the fluid sample to be tested is evenly discharged, perform relevant tests.

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