Pesticide composition containing fluorine indane pyraclostrobin

Abstract

The invention belongs to the technical field of pesticide compositions, and particularly relates to a pesticide composition containing fluorine indane pyraclostrobin. The invention relates to a pesticide composition, which is prepared by compounding fluindazole amine and Zhongshengmycin according to a mass ratio of (1-5): (25-1), or compounding fluindazole amine and physcion according to a mass ratio of (1-15): (35-1). According to the pesticide composition, when the fluindazole amine and the zhongshengmycin are compounded according to the mass ratio of (1-5): (25-1) and when the fluindazole amine and the physcion are compounded according to the mass ratio of (1-15): (35-1), a synergistic effect is achieved on crop diseases, and compared with any single agent of the fluindazole amine, the zhongshengmycin and the physcion, the pesticide composition has the advantages that the pesticide composition is safe and environment-friendly, and the pesticide effect is better. On the basis, the application amount of pesticides can be reduced, and pesticide residues and the prevention and treatment cost are reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compositions, and particularly relates to a pesticide composition containing fluinoxatamide. Background Art

[0002] Fluindapyr (CAS: 1383809-87-7) is a new pyrazole amide fungicide developed jointly by SEG in Italy and FMC in the United States. It is a type of succinate dehydrogenase inhibitor (SDHI) fungicide that works by inhibiting succinate dehydrogenase in the mitochondrial respiration of pathogens. Fluindapyr can be used in crops such as cereals, rice, soybeans, corn, vegetables and fruit trees to prevent and control diseases caused by pathogens such as Septoria, Alternaria, Cercospora and Coryneformis, such as powdery mildew, sheath blight, gray mold, sclerotinia and anthracnose.

[0003] Zhongshengmycin is an agricultural antibiotic fungicide developed by the Institute of Biocontrol of the Chinese Academy of Agricultural Sciences. It is produced by the Hainan variant of Streptomyces lilacinus and is an N-glycoside alkaline water-soluble substance. Zhongshengmycin has obvious antibacterial activity against crop pathogens such as cabbage soft rot, cucumber angular leaf spot, rice white leaf blight, apple ring rot and wheat fusarium. It inhibits the formation of peptide bonds in pathogenic bacterial proteins, leading to bacterial death; it can also inhibit the growth of fungal hyphae and spore germination, thereby achieving the effect of preventing and controlling fungal diseases; secondly, it can interact with plants to increase the activity of the main enzymes of plant defense reactions such as peroxidase, phenylalanine ammonia lyase and chalcone synthase, inducing plant disease resistance.

[0004] Physcion is a botanical fungicide independently developed in my country. It is made from natural rhubarb as raw material, and is processed and developed by extracting active ingredients. It belongs to the anthraquinone class of compounds. Physcion is a plant secondary metabolite that has the function of preventing and treating plant diseases, and has a good control effect on powdery mildew, downy mildew, gray mold and anthrax. In terms of mechanism of action, physcion interferes with the biosynthesis of chitin in the cell wall of pathogenic fungi, causing the germ tube and mycelium to swell and rupture locally, and the cell contents to discharge, leading to their death. In addition, it can inhibit the production of spores and the expansion of lesions, and has a friendly systemic conduction effect.

[0005] In the prior art, flutoxin, zhongshengmycin and physaccharin methyl ether are commonly used fungicides for preventing and controlling diseases on various crops, but in actual application, the prevention and control effect of a single agent is limited, and long-term use is prone to drug resistance. At present, there is no report on the composite composition of flutoxin and zhongshengmycin or physaccharin methyl ether in the prevention and control of crop diseases. Summary of the invention

[0006] The object of the present invention is to provide a pesticide composition containing flutoxafenamide to solve the problem that flutoxafenamide, cypermethrin and physcion are commonly used as fungicides for preventing and controlling diseases on various crops, but in actual application, the control effect of a single agent is limited and long-term use is prone to drug resistance.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A pesticide composition is prepared by compounding fluoxathiapiprolin and zhongshengmycin in a mass ratio of 1-5:25-1, or by compounding fluoxathiapiprolin and physaccharin methyl ether in a mass ratio of 1-15:35-1.

[0009] The effect is better, and the compounding mass ratio of flutoxafenamide to zhongshengmycin is 1:15.

[0010] In addition, the present invention also provides a pesticide preparation, which includes the pesticide composition.

[0011] Furthermore, in the pesticide preparation, the mass of the pesticide composition accounts for 0.1-85% of the total mass of the pesticide preparation, and the rest are auxiliary ingredients allowed to be added in pesticide science.

[0012] Furthermore, in the pesticide preparation, the mass of the pesticide composition accounts for 5-65% of the total mass of the pesticide preparation.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] When flutoxin and zhongshengmycin in the pesticide composition of the present invention are compounded at a mass ratio of 1-5:25-1, and when flutoxin and physaccharin methyl ether are compounded at a mass ratio of 1-15:35-1, a synergistic effect is shown on crop diseases. Compared with any single agent of flutoxin, zhongshengmycin and physaccharin methyl ether, the control effect on diseases can be improved, based on which the application amount of pesticides can be reduced, and the pesticide residues and control costs can be reduced. DETAILED DESCRIPTION

[0015] Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0016] 1. Indoor activity test of different compound combinations

[0017] 1.1 Test crops

[0018] Qinghai big red tomatoes, potted to the stage of 3-4 true leaves, 1 plant per pot.

[0019] 1.2 Test spore suspension

[0020] The Botrytis cinerea isolated from the diseased tomato plants was inoculated on the PDA medium. After spore production, the spores were washed with sterile water, filtered through double gauze, and prepared into 1×10 5 spores / mL of spore suspension.

[0021] PDA medium formula: 200g potato, 20g glucose, 15g agar powder, distilled water to 1000mL, natural pH.

[0022] 1.3 Test agents

[0023] 97% flufenacet technical (Qingdao Hengning Biotechnology Co., Ltd.), 3% zhongshengmycin aqueous solution (Sichuan Jinzhu Ecological Agriculture Technology Co., Ltd.), 2% physcion methyl ether water dispersible granules (Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.)

[0024] After the flufenacet original drug was dissolved in dimethyl sulfoxide, it was diluted with 0.1% Tween-80 aqueous solution to a high-concentration single-dose mother solution of 5000 mg / L; the Zhongshengmycin aqueous solution and the physcion methyl ether water-dispersible granules were diluted with 0.1% Tween-80 aqueous solution to a high-concentration single-dose mother solution of 5000 mg / L. According to the preliminary test, multiple groups of mass ratios were set, and each single-dose mother solution and each group of mixed agents were diluted into 5 mass concentration gradients using 0.1% Tween-80 aqueous solution. All the drugs were prepared and used immediately.

[0025] 1.4 Test method (refer to NY / T 1156.10-2008 "Guidelines for Indoor Bioassay of Pesticides - Fungicides Part 10: Pot Method for Control of Botrytis cinerea")

[0026] Spray the spore suspension onto the test crop, and then transfer to a dark incubator at 22°C and 95% relative humidity. After 24 hours, spray the solution evenly on the leaves until they are completely wet. After the solution is air-dried, transfer to 22°C, 85% relative humidity, and 12h alternating light and dark conditions for further cultivation. Set up 4 replicates for each mass concentration of solution, with 3 pots of tomato seedlings per replicate, and use the treatment of spraying 0.1% Tween-80 aqueous solution as a blank control.

[0027] When the diseased leaf rate of the blank control reaches more than 50%, the disease situation of each treatment is investigated in stages, and all leaves of each treatment are investigated. Based on the investigation results, the disease index and control effect of each treatment are calculated.

[0028]

[0029] 1.5 Classification Standards

[0030] Level 0: no lesions;

[0031] Level 1: The lesions occupy less than 5% of the entire leaf area;

[0032] Level 3: lesions occupy 5-15% of the entire leaf area;

[0033] Level 5: lesions occupy 15-25% of the entire leaf area;

[0034] Level 7: lesions occupy 25-50% of the entire leaf area;

[0035] Level 9: Lesions occupy more than 50% of the entire leaf area.

[0036] 1.6 Statistical analysis

[0037] DPS software was used to perform linear regression between the logarithmic value of fungicide concentration and the probability value of control efficacy to calculate the EC of each agent. 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC value) of the mixture, and the synergistic effect of the drug was evaluated based on the co-toxicity coefficient (CTC value): CTC ≤ 80 was antagonistic; CTC ≥ 120 was synergistic; 80 < CTC < 120 was additive. The results are shown in Table 1-2.

[0038] Table 1 Indoor activity test results of flutoxafenamide combined with zhongshengmycin against tomato gray mold

[0039]

[0040]

[0041] As shown in Table 1, when flufenacet and zhongshengmycin were mixed in a mass ratio of 1:25, 1:15, 1:5, 1:1 and 5:1, they showed a synergistic effect on tomato gray mold. When the mass ratio of the compound was 1:15, the synergistic effect of the compound was the most obvious.

[0042] Table 2 Indoor activity test results of flutoxacillin and physaccharin methyl ether against tomato gray mold

[0043]

[0044] As shown in Table 2, when fluinoxabiocin and physaccharin methyl ether were compounded in the mass ratios of 1:35, 1:25, 1:15, 1:5, 1:1, 5:1 and 15:1, they showed a synergistic effect on tomato gray mold.

[0045] In summary, when flutoxin and sinoprolin in the pesticide composition of the present invention are compounded in a mass ratio of 1-5:25-1, and when flutoxin and physaccharin methyl ether are compounded in a mass ratio of 1-15:35-1, a synergistic effect is exhibited on crop diseases. Compared with any single agent of flutoxin, sinoprolin and physaccharin methyl ether, the control effect on diseases can be improved, based on which the application amount of pesticides can be reduced, and pesticide residues and control costs can be reduced.

Claims

1. A pesticide composition, characterized in that: The pesticide composition is prepared by compounding fluoxathiapiprolin and zhongshengmycin in a mass ratio of 1-5:25-1, or by compounding fluoxathiapiprolin and physaccharin methyl ether in a mass ratio of 1-15:35-1.

2. The pesticide composition according to claim 1, characterized in that The composite mass ratio of flutoxafenamide to zhongshengmycin is 1:

15.

3. A pesticide preparation, characterized in that: The pesticide preparation comprises the pesticide composition according to claim 1.

4. The pesticide formulation according to claim 3, characterized in that In the pesticide preparation, the mass of the pesticide composition accounts for 0.1-85% of the total mass of the pesticide preparation, and the rest are auxiliary components allowed to be added in pesticide science.

5. The pesticide formulation according to claim 4, characterized in that: In the pesticide preparation, the mass of the pesticide composition accounts for 5-65% of the total mass of the pesticide preparation.

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