Pesticide composition for preventing and treating bactrocera cucurbitae

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating bactrocera cucurbitae. The invention relates to a pesticide composition with insecticidal activity. The pesticide composition with insecticidal activity is prepared by binary compounding of arfamarin and flubendiamide or tolfenpyrad, wherein the mass ratio of the arfamarin to the flubendiamide is (1-15): (9-1); the mass ratio of the arfamparin to the tetratolfenpyrad is (1-30): (30-1). According to the pesticide composition disclosed by the invention, the arfamparin and the flubendiamide or the tetratolfenpyrad with different action mechanisms are compounded, and the compounded composition shows a synergistic effect on killing the bactrocera cucurbitae, so that the control effect can be improved, the dosage of the pesticide can be reduced, and the pesticide cost can be reduced. Meanwhile, the compound can also be used for preparing stomach poison agents and poison baits with efficient insecticidal activity on the bactrocera cucurbitae, and has important significance on prevention and control of the bactrocera cucurbitae.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and controlling bitter melon fruit flies. Background Art

[0002] The melon fruit fly (Bactrocera cucurbitae) is one of the important pests of melon and fruit crops. The loss of melons and fruits damaged by the melon fruit fly is generally more than 30%, among which bitter melon is the most seriously damaged. In recent years, the excessive use of pesticides for the control of melon fruit flies in production has caused serious pollution to the environment, and has caused melon fruit flies to develop different degrees of resistance to pesticides. At the same time, there is also the problem of pesticide residues. With the improvement of people's awareness of ecological safety, the control of melon fruit flies by using stomach poison agents processed into poison baits has gradually become the main means of control strategy. Compared with contact-type agents, the poison baits processed from stomach poison agents use less medicine, have strong target properties, are safe for non-target organisms, and have little pollution to the environment. Based on this, screening stomach poison agents with high insecticidal activity against melon fruit flies and processing them into poison baits is of great significance for the prevention and control of melon fruit flies.

[0003] Afoxolaner is a new type of isoxazoline insecticide and acaricide successfully developed by DuPont in the United States in 2008. CAS: 1093861-60-9, molecular formula: C 26 H 17 ClF9N3O3, relative molecular weight: 625.870, structural formula is as follows:

[0004]

[0005] Afolanol acts on ligand-gated chloride ion channels, especially inhibiting channels gated by the neurotransmitter γ-aminobutyric acid (GABA), blocking the transmission of chloride ions from the presynaptic membrane to the postsynaptic membrane, resulting in increased neuronal activity in insects and excessive excitement and death. The patent CN11838337A discloses a composition containing afolanol and cyantraniliprole, which discloses that afolanol and cyantraniliprole have a synergistic effect when used to control agricultural insects after compounding. There is no report on the binary compounding of afolanol with bromobenzene or tetrazolyl cypermethrin. Summary of the invention

[0006] The object of the present invention is to provide a pesticide composition for controlling bitter melon fruit flies, so as to solve the problem caused by excessive use of pesticides for controlling bitter melon fruit flies in production.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A pesticide composition with insecticidal activity, which is formed by a binary compound of afoxolan and brofenoxamide or tetrazolyl pyrifos; wherein the mass ratio of afoxolan to brofenoxamide is 1-15:9-1; and the mass ratio of afoxolan to tetrazolyl pyrifos is 1-30:30-1.

[0009] The effect is better, and the mass ratio of afoxlan to bromofenac is 1:3.

[0010] The effect is better, and the mass ratio of afolan to tetrazobactam is 1:7.

[0011] The present invention also provides application of the insecticidal pesticide composition in preventing and controlling bitter melon fruit flies.

[0012] The present invention also provides the use of the insecticidal pesticide composition in preparing a stomach poison agent or poison bait agent for bitter melon fruit flies.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The pesticide composition of the present invention compounds afoxanil with bromofenac or tetrazobactam, which have different action mechanisms. The compound combination shows a synergistic effect in killing bitter melon fruit flies, can improve the control effect, and can reduce the amount of the agent used. At the same time, it can also be used to prepare stomach poison agents and poison bait agents with high insecticidal activity against bitter melon fruit flies, which is of great significance for the prevention and control of bitter melon fruit flies. DETAILED DESCRIPTION

[0015] The technical solution of the present invention is described clearly and completely below.

[0016] Example :Indoor biological activity test

[0017] 1. Test pests

[0018] After the damaged bitter melons were collected, the melon fruit fly larvae were picked out from them. After several generations of successive breeding with fresh and clean bitter melons, healthy adults of the same batch at 3-4 days old after emergence were selected as test subjects.

[0019] Rearing conditions: temperature 25±1℃, relative humidity 70±5%, photoperiod 16L / 8D.

[0020] 2. Test reagents

[0021] 98% Afolan technical, 98% Bromofenac technical, 90% Tetrazodone technical.

[0022] 3. Test methods

[0023] Liquid feeding method: After dissolving the test agent, dilute it with 10% sucrose solution (the solvent is 0.1% Tween-80 aqueous solution) to form a single-dose stock solution; set up multiple groups of ratios, and dilute each single dose and each group of mixed ratios with 10% sucrose solution to 5 mass concentration gradients for use.

[0024] Completely immerse a disinfected sponge of 2cm×2cm×2cm in the liquid medicine for 30 seconds and then take it out. When the liquid medicine stops dripping, place the sponge in a conical flask. Introduce 20 test pests that have been starved for 24 hours with clean water into the conical flask, and seal the mouth of the flask with gauze to prevent escape. Continue to raise them under the conditions of temperature 25±1℃, relative humidity 70±5%, and photoperiod 16L / 8D, and repeat 3 times for each concentration. The treatment with 10% sucrose solution was used as the blank control. Observe the death of the test insects 36 hours after treatment, record the total number of insects and the number of dead insects in each treatment, and calculate the corrected mortality rate of each treatment. Among them, the failure of the test pest to turn over within 30 seconds was used as the criterion for determining death.

[0025]

[0026] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0027]

[0028] In the above formula: P1--corrected mortality rate, unit is %; P t --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0029] 4. Data Analysis

[0030] DPS software was used to perform regression analysis on the logarithmic values ​​of the concentration of each treatment agent and the corrected mortality probability value of each treatment to calculate the LC of each treatment agent. 50 Then the Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC value) of the mixture.

[0031] 5. Efficacy evaluation

[0032] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0033] Table 1 The results of toxicity test of afolan and bromofenac against bitter melon fruit flies

[0034]

[0035] As shown in Table 1, the co-toxicity coefficients of afoxolana and bromofenac in the mass ratio range of 1-15:9-1 are all greater than 120, that is, the biological activity of afoxolana and bromofenac against bitter melon fruit flies is synergistic. The synergistic effect is particularly obvious when the mass ratio is 1:3, reaching 459.119.

[0036] Table 2 The results of toxicity test of afolan and tetrazobacteramide against bitter melon fruit flies

[0037]

[0038]

[0039] As shown in Table 2, the co-toxicity coefficients of afoulana and tetrazolyl thiocarb in the mass ratio range of 1-30:30-1 were all greater than 120, that is, the biological activity of afoulana and tetrazolyl thiocarb against bitter melon fruit flies showed a synergistic effect. The synergistic effect was particularly obvious when the mass ratio was 1:7, reaching 340.954.

[0040] In summary, the pesticide composition of the present invention compounds afoxanil with bromofenac or tetrazobactam, which have different action mechanisms. The compound combination shows a synergistic effect in killing bitter melon fruit flies, can improve the control effect, and can reduce the amount of the agent used. At the same time, it can also be used to prepare stomach poison agents and poison baits with high insecticidal activity against bitter melon fruit flies, which is of great significance for the prevention and control of bitter melon fruit flies.

Claims

1. An insecticidal pesticide composition, characterized in that: The insecticidal pesticide composition is formed by a binary compound of afoxolan and brofenoxamide or tetrazolyl pyrifos; wherein the mass ratio of afoxolan to brofenoxamide is 1-15:9-1; and the mass ratio of afoxolan to tetrazolyl pyrifos is 1-30:30-1.

2. The insecticidal pesticide composition according to claim 1, characterized in that The mass ratio of afoxlan to bromofenac is 1:

3.

3. The insecticidal pesticide composition according to claim 1, characterized in that: The mass ratio of afolanine to tetrazobactam is 1:

7.

4. Use of the insecticidal pesticide composition according to any one of claims 1 to 3 in controlling bitter melon fruit flies.

5. Use of the insecticidal pesticide composition according to any one of claims 1 to 3 in the preparation of a stomach poison or bait for bitter melon fruit flies.

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