Hyaluronic acid composition with instantaneous tightening effect and application and cosmetics thereof

Abstract

The invention belongs to the field of cosmetics, and discloses a hyaluronic acid composition with an instantaneous tightening effect and application of the hyaluronic acid composition, and the hyaluronic acid composition comprises the following components: 0.1-1 part of hyaluronic acid, 0.1-0.5 part of carboxymethyl chitosan and 0.05-0.5 part of an algae extract. The hyaluronic acid in the hyaluronic acid composition is matched with the carboxymethyl chitosan and the algae extract, so that the instant tightening and anti-wrinkle effects can be achieved; in addition, the invention also discloses a cosmetic which contains 0.2 wt%-1.0 wt% of the hyaluronic acid composition.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a hyaluronic acid composition with instant firming effect, application thereof, and cosmetics. Background Art

[0002] As we age, our metabolism slows down, our water content and subcutaneous fat decrease, elastic fibers become thicker, our skin wrinkles, and our luster fades. Skin aging is a prominent manifestation of aging of the entire body, especially on the face. The number of fibroblasts in the body decreases, and the collagen and elastin they secrete also decrease and break due to oxidation, which causes the epidermis to lose its support, resulting in uneven collapse. The dermis begins to thin, the skin's elasticity deteriorates, and wrinkles begin to appear. In addition to natural physiological factors, the aging of human skin is also related to factors such as long-term sleep quality, ultraviolet radiation, working environment, living habits, nutritional status, and skin care.

[0003] The application of algae extracts in anti-wrinkle is disclosed by a large number of prior arts, such as:

[0004] Chinese patent application 202010722691.1 discloses an anti-wrinkle composition and its preparation method and application, and Chinese patent application 202211195178.7 discloses a three-algae soothing skin care product and its application in inhibiting the expression of sensitive factors;

[0005] The above patent application uses algae extract to achieve the improvement of skin's anti-wrinkle and other performances.

[0006] During the research of this project, we found that algae extracts and skin can be combined through ionic bonding. Therefore, based on this characteristic, improving the application effect of algae extracts from the perspective of film formation is an optional application direction. Summary of the invention

[0007] In order to solve the above problems, the present invention provides a method for preparing a hyaluronic acid composition with instant firming effect, which achieves the effect of instant skin firming by combining hyaluronic acid, carboxymethyl deacetylated chitosan and algae extract.

[0008] To achieve the above-mentioned purpose, the present application discloses a hyaluronic acid composition with instant firming effect, which comprises the following components by weight:

[0009] 0.1-1 part of hyaluronic acid;

[0010] Polysaccharides 0.1-0.5 parts;

[0011] 0.05-0.5 parts of algae extract;

[0012] The polysaccharide substance is carboxymethyl deacetylated chitosan.

[0013] Hyaluronic acid is a natural ingredient widely found in the human body, which has significant anti-wrinkle and moisturizing effects. Therefore, adding hyaluronic acid can fill the air gaps between cells, make the skin plump and elastic, and reduce the appearance of wrinkles. It can also form a barrier on the skin surface to prevent moisture loss.

[0014] Carboxymethyl chitosan has a high inhibition rate on a variety of inflammatory factors, can effectively repair cells, reduce skin redness, itching and inflammatory reactions, and also has a certain moisturizing effect;

[0015] Algae extract has a good antioxidant effect. It absorbs free radicals to protect biological macromolecules from attack, thereby removing free radicals and slowing down the aging of the skin. Algae extract can also stimulate the production of collagen and enhance skin elasticity, thereby achieving the effect of firming the skin.

[0016] Through the synergistic effect of hyaluronic acid's instant filling effect, algae extract's antioxidant effect, and carboxymethyl chitosan's cell repair effect, the skin can achieve an instant firming effect.

[0017] Preferably, the molecular weight of the carboxymethyl chitosan is 9,000-11,000.

[0018] Preferably, the algae extract is selected from at least one of a red algae extract and a brown algae extract.

[0019] Further preferably, the algae extract is a mixture of a red algae extract and a brown algae extract, and the mass ratio of the red algae extract to the brown algae extract is 0.08-0.20:0.08-0.20.

[0020] Preferably, the hyaluronic acid is hyaluronic acid with a total molecular weight and an average molecular weight of 600±10 kDa.

[0021] Preferably, the preparation method of the hyaluronic acid composition with instant firming effect is: mixing and dispersing hyaluronic acid, polysaccharide substances and algae extracts to obtain the hyaluronic acid composition.

[0022] In addition, the invention also discloses the use of the hyaluronic acid composition with instant firming effect for preparing cosmetics.

[0023] In addition, a cosmetic is disclosed, comprising 0.2 wt% to 1.0 wt% of the above hyaluronic acid composition with instant firming effect;

[0024] Preferably, the cosmetic is essence water or essence milk.

[0025] The beneficial effects of the present invention are:

[0026] The present invention provides a method for preparing a hyaluronic acid composition with instant firming effect. The composition comprises hyaluronic acid, carboxymethyl chitosan and algae extract. The instant filling effect of hyaluronic acid, the antioxidant effect of algae extract and the cell repair effect brought by carboxymethyl chitosan interact with each other to achieve an instant firming effect on the skin. DETAILED DESCRIPTION

[0027] In the description of the present invention, it should be noted that, if the specific conditions are not specified in the examples, the experiments were carried out according to conventional conditions or the conditions recommended by the manufacturer. The reagents or instruments used, if the manufacturer is not specified, are all conventional products that can be purchased commercially.

[0028] Product information:

[0029] Hyaluronic acid with full molecular weight and average molecular weight of 200±10 kDa: provided by Shandong Guantianxia Biotechnology Co., Ltd., model or brand: full molecular weight distribution sodium hyaluronate-20 (version number 202203);

[0030] Hyaluronic acid with a total molecular weight and an average molecular weight of 600±10 kDa: Shandong Guantianxia Biotechnology Co., Ltd., model or brand: GD-HA60 (version number 202202);

[0031] Carboxymethyl chitosan (MHA): Qingdao Boyite Biomaterial Co., Ltd.;

[0032] Brown algae extract (Antarctic ice sea brown algae): provided by Guangzhou Huilang Biotechnology Co., Ltd., with an active ingredient content of 98%;

[0033] Red algae extract: provided by Shanghai Jiayu Biotechnology Co., Ltd., with an active ingredient content of 80%;

[0034] Chlorella extract: provided by Wuhan Baixing Biotechnology Co., Ltd., item number: 101;

[0035] Euglena extract: provided by Shanxi Ruisi Biotechnology Co., Ltd., catalog number: RS-0516-458;

[0036] Chitosan oligosaccharide: provided by Shandong Hailongyuan Biotechnology Co., Ltd., with a molecular weight of less than 1610.

[0037] Unless otherwise specified, the preparation methods of a hyaluronic acid composition with instant firming effect in the following examples and comparative examples are:

[0038] The hyaluronic acid, polysaccharide substances and algae extract are mixed according to mass fractions, stirred and dispersed to obtain a hyaluronic acid composition with instant firming effect.

[0039] Examples 1-7

[0040] The formula of a hyaluronic acid composition with instant firming effect is shown in Table 1 by weight:

[0041] Table 1

[0042]

[0043] Wherein, the polysaccharide substance described in Examples 1-7 is carboxymethyl deacetylated chitosan with a molecular weight of 10250±205;

[0044] The hyaluronic acid is a full molecular weight hyaluronic acid with an average molecular weight of 600±10 kDa.

[0045] Embodiment 8-13

[0046] The formula of a hyaluronic acid composition with instant firming effect is shown in Table 2 by weight:

[0047] Table 2

[0048]

[0049] In Example 8, the hyaluronic acid is a hyaluronic acid with a total molecular weight and an average molecular weight of 200±10 kDa;

[0050] The hyaluronic acid in Example 9 is a commercially available hyaluronic acid with a medium molecular weight (molecular weight span is 10 to 1000 kDa) and an average molecular weight of 600 ± 10 kDa;

[0051] The hyaluronic acid in Examples 10-11 is a full molecular weight hyaluronic acid having an average molecular weight of 600±10 kDa;

[0052] The polysaccharide substance in Examples 8-11 is carboxymethyl deacetylated chitosan with a molecular weight of 10250±205.

[0053] Comparative Examples 1-4

[0054] The method is basically the same as Example 1, except that the formula of the hyaluronic acid composition is calculated by weight as shown in Table 3:

[0055] Table 3

[0056]

[0057]

[0058] Comparative Example 5

[0059] The method is substantially the same as Example 1, except that chitosan oligosaccharide is used to replace carboxymethyl deacetylated chitosan.

[0060] Performance Testing

[0061] 1. Instant firming and anti-wrinkle test:

[0062] Human test: The purpose of this test is to evaluate the instant lifting, anti-wrinkle, and reduction of roughness and crow's feet area and number effects of a cosmetic ingredient. The research subjects are five volunteers in a group. Each group of volunteers uses a 0.6wt% aqueous solution of the hyaluronic acid composition prepared in any one of Examples 1-13 or Comparative Examples 1-5 on one side of their face, and directly uses clean water on the other side of their face. The average value of the measurement results of the five volunteers is the final result of the group.

[0063] Bioassay Assessment:

[0064] use 1000D took macro images of the face at T = 0, T = 30 min, and T = 60 min;

[0065] The number and area of ​​crow's feet and the roughness of the skin will be assessed by PRIMOS Premium at T=0, T=30min and T=60min respectively.

[0066] The smaller the F4 measurement value (skin firmness parameter F4 value), the better the skin firmness, and the smaller the F4 measurement value, the shorter the length of the skin wrinkles at the crow's feet. The skin firmness parameter F4 measurement values ​​are shown in Table 4:

[0067] Table 4

[0068]

[0069]

[0070] The measured values ​​of skin wrinkle length are shown in Table 5:

[0071] Table 5

[0072]

[0073]

[0074] Conclusion analysis:

[0075] 1. As can be seen from Table 4, when the mass ratio of hyaluronic acid, polysaccharide and red algae extract was slightly changed in Examples 1-3, the change rate of the skin firming parameter F4 value of Examples 1-3 showed certain fluctuations. At the same time, the change rate of the skin firming parameter F4 value of Example 1 was greater than that of Examples 2 and 3, whether at 30 minutes or at 60 minutes. It can be seen that the mass ratio of hyaluronic acid, polysaccharide and red algae extract in the formula of Example 1 is more advantageous.

[0076] Further observation of Table 5 shows that the formula of Example 1 also has advantages over Examples 2 and 3 in the test of skin wrinkle length change rate;

[0077] Taking the skin firming parameter F4 value change rate of Example 1 as an example, within the first 30 minutes of use, the skin firming parameter F4 value decreased by 14.17% compared with before use;

[0078] After 60 minutes of use, the skin firmness parameter F4 value decreased by 21.00% compared with before use;

[0079] It can be seen that the composition has a good firming effect within the first 30 minutes, and thus the composition of the present application has a good instantaneous firming ability. Similarly, we still cannot deny that the composition also has a good long-term moisturizing ability;

[0080] 2. It can be seen from Examples 1 and 4 that when the red algae extract is replaced by the brown algae extract, Example 1 has a certain degree of advantage over Example 4 in terms of the change rate of the skin firming parameter F4 value and the change rate of the skin wrinkle length, but the advantage is not obvious;

[0081] Further observation of Example 5 shows that when the red algae extract and the brown algae extract are compounded for use in Example 5, Example 5 is significantly superior to Example 1 and Example 4 in terms of the change rate of the skin firming parameter F4 value and the change rate of the skin wrinkle length. It can be seen that when the red algae extract and the brown algae extract are compounded for use, the two can produce a synergistic effect to further enhance the firming and anti-wrinkle ability of the composition.

[0082] While Examples 6-7 also use a compound of red algae extract and brown algae extract, but it can be seen from Tables 4-5 that although Examples 6-7 still have advantages over Examples 1 and 4, their advantages are not as obvious as those of Example 5. It can be seen that the mass ratio of hyaluronic acid, carboxymethyl deacetylated chitosan, red algae extract, and brown algae extract in Example 5 is more advantageous;

[0083] 3. It can be seen from Examples 1 and 8-9 that when HA20 (hyaluronic acid with a total molecular weight and an average molecular weight of 200±10 kDa) is used to replace HA60 (hyaluronic acid with a total molecular weight and an average molecular weight of 600±10 kDa) in Example 1, the change rate of the skin firming parameter F4 value and the change rate of the skin wrinkle length in Example 8 are lower than those in Example 1;

[0084] At the same time, when Example 9 uses medium molecular weight hyaluronic acid instead of full molecular weight hyaluronic acid in Example 1, the change rate of skin firming parameter F4 value and skin wrinkle length in Example 9 are also reduced compared with Example 1. It can be seen that the average molecular weight of hyaluronic acid and the molecular weight distribution range of hyaluronic acid also have an important influence on the firming and anti-wrinkle ability of the composition.

[0085] 4. It can be seen from Examples 1 and 10-11 that when the red algae extract is replaced by the green algae extract or the euglena extract, the skin firming parameter F4 value change rate and the skin wrinkle length change rate of Examples 10 and 11 are lower than those of Example 1. Therefore, it can be seen that the red algae extract is irreplaceable.

[0086] 5. It can be seen from Example 1 and Comparative Examples 1-3 that when any one of hyaluronic acid, carboxymethyl chitosan and algae extract is missing from the composition, the firming and anti-wrinkle abilities of Comparative Examples 1-3 show a significant downward trend compared to Example 1. It can be seen that any one of the above three is indispensable;

[0087] Further calculations of Comparative Examples 1-3 show that there is a synergistic effect between hyaluronic acid, carboxymethyl chitosan and algae extract, which greatly improves the firming and anti-wrinkle ability of the composition. The reason is that: taking the change rate of the skin firming parameter F4 value in 30 minutes as an example, when comparative example 1 uses carboxymethyl chitosan and algae extract at the same time, the change rate of the skin firming parameter F4 value in 30 minutes is -2.79%;

[0088] When hyaluronic acid and algae extract were used simultaneously in comparative example 2, the change rate of the skin firming parameter F4 value was -2.04% in 30 minutes;

[0089] When hyaluronic acid and carboxymethyl chitosan were used simultaneously in comparative example 3, the change rate of the skin firming parameter F4 value was -2.98% in 30 minutes;

[0090] It can be seen that, theoretically, if the firming ability of Example 1 is derived from the simple superposition of hyaluronic acid, carboxymethyl chitosan, and algae extract, then the change rate of the skin firming parameter F4 value of Example 1 in 30 minutes is -3.905%;

[0091] However, the actual 30-minute skin firming parameter F4 value change rate of Example 1 was -14.17%;

[0092] It can be seen that the firming ability achieved by Example 1 far exceeds the simple superposition of hyaluronic acid, carboxymethyl chitosan and algae extract;

[0093] 6. It can be seen from Example 1 and Comparative Example 5 that when the polysaccharide (carboxymethyl deacetylated chitosan) is replaced by other saccharides, the change rate of the skin firming parameter F4 value and the change rate of the skin wrinkle length of Comparative Example 5 are significantly reduced compared with those of Example 1. It can be seen that the polysaccharide in the composition is difficult to be replaced by other saccharides.

[0094] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principles of the present invention shall be equivalent replacement methods and shall be included in the protection scope of the present invention.

Claims

1. A hyaluronic acid composition having instant firming effect, characterized in that: By mass, it contains the following ingredients: 0.1-1 part of hyaluronic acid; Polysaccharides 0.1-0.5 parts; 0.05-0.5 parts of algae extract; The polysaccharide substance is carboxymethyl deacetylated chitosan.

2. The hyaluronic acid composition with instant firming effect according to claim 1, characterized in that: The molecular weight of the carboxymethyl chitosan is 9000-11000.

3. The hyaluronic acid composition with instant firming effect according to claim 1, characterized in that: The algae extract is selected from at least one of a red algae extract and a brown algae extract.

4. The hyaluronic acid composition with instant firming effect according to claim 3, characterized in that: The algae extract is a mixture of a red algae extract and a brown algae extract, and the mass ratio of the red algae extract to the brown algae extract is 0.08-0.20:0.08-0.

20.

5. The hyaluronic acid composition with instant firming effect according to claim 1, characterized in that: The hyaluronic acid has a total molecular weight and an average molecular weight of 600±10 kDa.

6. The hyaluronic acid composition with instant firming effect according to claim 1, characterized in that: The preparation method of the hyaluronic acid composition with instant firming effect is: hyaluronic acid, polysaccharide substances and algae extracts are mixed and dispersed to obtain the hyaluronic acid composition.

7. Use of the hyaluronic acid composition with instant firming effect as claimed in any one of claims 1 to 6 for preparing cosmetics.

8. A cosmetic, characterized in that: The composition comprises 0.2 wt % to 1.0 wt % of the hyaluronic acid composition with instant firming effect as claimed in any one of claims 1 to 6.

9. The cosmetic according to claim 8, characterized in that: The cosmetic is essence water or essence milk.

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