Vinyltrimethylsilane-modified gelatin, and preparation method and application thereof

Abstract

This invention provides a vinyltrimethylsilane-modified gelatin, its preparation method, and its application, belonging to the field of gelatin modification technology. Vinyltrimethylsilane is used to modify gelatin. In addition to having strong hydrophobicity and antioxidant properties, the modified gelatin also exhibits better resistance to external forces, significantly improved toughness and rigidity, and can promote cell migration, achieving a cell migration rate of 60-65.4% for epidermal cells, thus accelerating the healing of skin wounds.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, specifically to a vinyltrimethylsilane-modified gelatin, its preparation method, and its applications. Background Technology

[0002] Gelatin is a derivative of collagen, a major structural and connective protein in animals. Derived from denatured collagen, gelatin contains polypeptide sequences with Gly-XY repeats, where X and Y are most commonly proline and hydroxyproline residues. These sequences form a triple helix structure and influence the gelling ability of gelatin polypeptides. Currently available gelatin is extracted through processing from the hides and bones of animals typically derived from cattle and pigs. Gelatin's biophysical properties make it a versatile material, widely used in medicine, imaging, industry, cosmetics, and food and beverage fields, making it a valuable and multi-purpose product.

[0003] To obtain gelatin products with superior performance, several reports have documented methods for modifying or optimizing gelatin. Among them, the invention patent application with publication number "CN107519035A" (application number: 201710766008.2) discloses the application of a gelatin-hydrolyzed gelatin compound in skin care products. After being added to cosmetics, this compound has greater skin permeability due to the similarity between gelatin and protein structures. It can promote the synthesis of collagen in the skin, replenish skin collagen, and achieve the effect of skin health and restoration of elasticity. The invention patent with publication number "CN107513173A" (application number: 201710731344.3) discloses a method for preparing organosilicon-modified collagen membrane. This method involves first completely dissolving animal collagen in acetic acid solution to prepare a collagen protein solution. After removing air bubbles, the solution is cast into a film and dried to obtain a white, transparent collagen membrane. Then, at room temperature, the prepared collagen membrane is placed in an inorganic alkaline solution, and organosilicon is added to initiate the reaction. After the reaction, the surface is rinsed and dried to obtain the modified collagen membrane. The resulting collagen membrane has significantly improved hydrophobicity, essentially eliminating obstacles to the commercial application of collagen membranes and preventing exogenous toxic chemicals from entering the collagen membrane and affecting its edibility. Both of the above patents describe methods for compounding or modifying gelatin, resulting in significant improvements in skincare and other aspects of modified gelatin. However, currently, there are still relatively few records regarding methods and applications of gelatin modification.

[0004] Vinyltrimethylsilane is a colorless, transparent liquid with an ester-like odor. It plays a significant role in modern pharmaceutical synthesis, undergoing electrophilic substitution and addition reactions equivalent to ethylene. It can also be used to prepare various unsaturated aryl derivatives, silyl etherification reagents, unsaturated aldehydes, chiral three-membered rings, and for stereoselective hydrogenation. Furthermore, vinyltrimethylsilane is a silane coupling agent; it is a compound composed of organic groups and silicon. Its molecule contains different reactive groups capable of chemically binding with both inorganic and organic materials. When used for material modification, it can increase the compatibility between inorganic fillers and polymeric organic materials, significantly improving the performance of composite materials. For example, the invention patent with publication number "CN104945563A" (application number: 201510406043.4) introduces vinyltrimethylsilane for grafting through in-situ chlorination grafting. A dispersant is used to make the reaction more complete. The in-situ chlorination grafting reaction of PVC is adopted. Compared with general graft copolymers, vinyltrimethylsilane can further improve the polarity and comprehensive performance of the polymer, and also increase the mechanical properties and heat resistance of the modified PVC resin.

[0005] To further explore more applications of vinyltrimethylsilane and more modification methods for gelatin, this invention proposes to modify gelatin with vinyltrimethylsilane and to find more potential applications of vinyltrimethylsilane in different fields. Summary of the Invention

[0006] To address the aforementioned problems, this invention provides a vinyltrimethylsilane-modified gelatin, its preparation method, and its application. The gelatin is modified with vinyltrimethylsilane. In addition to possessing strong hydrophobicity and antioxidant properties, the modified gelatin exhibits better resistance to external forces, significantly improved toughness and rigidity, and promotes cell migration, achieving a cell migration rate of 60-65.4% for epidermal cells, thus accelerating the healing process of skin wounds.

[0007] The technical solution of this invention is as follows:

[0008] A vinyltrimethylsilane-modified gelatin, wherein vinyltrimethylsilane is used to modify gelatin, and the tensile strength of the vinyltrimethylsilane-modified gelatin is 15–19.3 N·mm. 2 It has a Young's modulus of 10–14.8 kPa, an elongation at break of 20–25.2%, a contact angle of 90–98°, a scavenging rate of 90–98.6% for hydroxyl radicals, a scavenging rate of 73–80.4% for DPPH radicals, a scavenging rate of 85–91.5% for superoxide radicals, and a cell migration rate of 60–65.4% for epidermal cells.

[0009] Preferably, the infrared spectrum of the vinyltrimethylsilane-modified gelatin is within 1140 cm⁻¹.-1 The Si-O characteristic peaks appeared, as well as at 3110 cm⁻¹. -1 Amide II band at NH bond bending vibration, 3020 cm -1 The stretching vibration peak of the CH bond in the olefin, 1590 cm⁻¹ -1 Amide II band at NH bond bending vibration, 1480 cm -1 Amide III band of CN bond stretching vibration at 1360 cm⁻¹ -1 1300cm -1 The symmetric stretching vibration peak of the methyl CH bond, 979 cm⁻¹ -1 The bending vibration peak of the CH bond.

[0010] The method for preparing vinyltrimethylsilane-modified gelatin involves modifying gelatin with vinyltrimethylsilane and using tetrabutylammonium iodide and triethylamine as catalysts in the modification reaction to obtain vinyltrimethylsilane-modified gelatin.

[0011] Preferably, the preparation method specifically includes the following steps:

[0012] (1) Prepare a 3-5% wt gelatin solution, let it stand at room temperature, put the gelatin solution into a 42-48℃ water bath and stir for 10-15 min, and adjust the pH to 9-10;

[0013] (2) Tetrabutylammonium iodide was added to the gelatin solution in step (1) under oxygen-free conditions to obtain reactant 1;

[0014] (3) Dissolve vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, add vinyltrimethylsilane solution to reactant 1, mix thoroughly to obtain reactant 2;

[0015] (4) Prepare a mixed solvent by mixing dimethyl sulfoxide and water in a ratio of 5-7:4-5 (v / v), dissolve triethylamine in the mixed solvent to obtain a triethylamine solution, add the triethylamine solution to reactant 2, and stir the reaction at 42-48°C for 40-50 h to obtain reactant 3;

[0016] (5) Pour reactant 3 into a mold and dry it in an oven at 40-45°C under normal pressure. Then rinse it with acetone and dry it again to obtain vinyltrimethylsilane modified gelatin.

[0017] Preferably, the room temperature standing time in step (1) is more than 1 hour;

[0018] Preferably, the method for isolating oxygen in step (2) is as follows: nitrogen gas is continuously introduced into the gelatin solution of step (1) for 10 minutes, then tetrabutylammonium iodide is added, and nitrogen gas is continued to be introduced for 30 minutes.

[0019] Preferably, the weight ratio of gelatin, tetrabutylammonium iodide, vinyltrimethylsilane, and triethylamine is 30-40:1:5:1.

[0020] The application of vinyltrimethylsilane-modified gelatin in improving the tensile strength of gelatin.

[0021] The application of the vinyltrimethylsilane-modified gelatin in promoting wound healing.

[0022] The application of vinyltrimethylsilane-modified gelatin in improving the antioxidant properties of gelatin.

[0023] The application of vinyltrimethylsilane-modified gelatin in improving the hydrophobicity of gelatin.

[0024] The application of the vinyltrimethylsilane-modified gelatin in the preparation of skin care products.

[0025] Preferably, the skincare products include toners, lotions, moisturizing creams, hand creams, foot creams, bath products, shampoos, and hair care products, and the dosage forms of the skincare products include liquids, lotions, creams, gels, and masks.

[0026] Beneficial effects:

[0027] This invention provides a vinyltrimethylsilane-modified gelatin, its preparation method, and its application. The gelatin is modified with vinyltrimethylsilane. In addition to having strong hydrophobicity and antioxidant properties, the modified gelatin also has better resistance to external forces, significantly improved toughness and rigidity, and can promote cell migration. The cell migration rate of epidermal cells is 60-65.4%, which accelerates the healing of skin wounds. Attached Figure Description

[0028] Figure 1 Infrared spectral analysis results of gelatin before modification, vinyltrimethylsilane, and vinyltrimethylsilane-modified gelatin;

[0029] Figure 2 The results of hydrogen spectroscopy for gelatin before modification, vinyltrimethylsilane, and vinyltrimethylsilane-modified gelatin;

[0030] Figure 3 Stress-strain curves of gelatin before modification and vinyltrimethylsilane-modified gelatin;

[0031] Figure 4 The results of cytotoxicity tests are for gelatin before modification and gelatin modified with vinyltrimethylsilane.

[0032] Figure 5 The results show the hydroxyl radical scavenging rates of gelatin before modification and vinyltrimethylsilane-modified gelatin.

[0033] Figure 6 The results of the detection of DPPH free radical scavenging rate of gelatin before modification and vinyltrimethylsilane modified gelatin;

[0034] Figure 7 The results of superoxide radical scavenging rate tests were obtained for gelatin before modification and gelatin modified with vinyltrimethylsilane.

[0035] Figure 8 Images of cell scratches at 0h and 24h;

[0036] Figure 9 Cell migration rates of gelatin before modification and vinyltrimethylsilane-modified gelatin at 24 h;

[0037] Figure 10 For stability testing of moisturizing face cream by centrifugation experiment;

[0038] Figure 11 To test the stability of moisturizing face cream against heat, cold, and alternating hot and cold conditions;

[0039] Figure 12 Patch test for evaluating the safety of moisturizing face cream;

[0040] Figure 13 The results of an experiment on the moisturizing effect of a moisturizing face cream on the skin;

[0041] Figure 14 The results of an experiment on the effects of moisturizing face cream on skin elasticity;

[0042] Figure 15 The results of using the moisturizing face cream for 28 days showed improvement in skin roughness;

[0043] Figure 16 The results of using a moisturizing face cream for 14 days showed improvement in skin roughness; Detailed Implementation

[0044] The following description is based on specific embodiments:

[0045] Explanation of the source of experimental materials:

[0046] CCK-8 test solution: purchased from Shanghai Weihuan Biotechnology Co., Ltd.;

[0047] Human skin fibroblasts: purchased from Pronosei Biotechnology Co., Ltd.;

[0048] Hacat cells: purchased from Aibiwei Biotechnology;

[0049] Electronic universal tensile testing machine: purchased from Xinte Testing Machine Co., Ltd.;

[0050] Contact angle measuring instrument: purchased from Shengding Precision Instruments Co., Ltd.;

[0051] ELISA reader: purchased from Ruifudi Biomedical (Shanghai) Co., Ltd.;

[0052] Coreometer CM825 skin moisture content testing probe: purchased from Shanghai Tusen Vision Technology Co., Ltd.;

[0053] Cutometer MPA580 instrument probe: purchased from Shanghai Tusen Vision Technology Co., Ltd.;

[0054] Skin surface texture testing system: purchased from Shanghai Tusen Vision Technology Co., Ltd.;

[0055] VisioScan VC 20plus: Purchased from Shanghai Tusen Vision Technology Co., Ltd.

[0056] Example 1:

[0057] Preparation of vinyltrimethylsilane modified gelatin

[0058] The specific steps are as follows:

[0059] (1) Weigh 5.00g of gelatin and put it into a 250mL three-necked flask. Add 95mL of water to prepare a 5%wt gelatin solution. After standing at room temperature for 1h, place the three-necked flask containing the gelatin solution into a 45℃ water bath and stir for 10min. Adjust the pH to 9.0.

[0060] (2) Nitrogen gas was continuously introduced into the gelatin solution of step (1) for 10 min, then 0.13 g of tetrabutylammonium iodide was added, and nitrogen gas was continued to be introduced for 30 min to obtain reactant 1;

[0061] (3) Dissolve 0.70g of vinyltrimethylsilane in 5mL of dimethyl sulfoxide to obtain vinyltrimethylsilane solution. Transfer the vinyltrimethylsilane solution to a constant pressure dropping funnel and add it dropwise to reactant 1 at a rate of 1d / 2s to obtain reactant 2.

[0062] (4) Take 6 mL of dimethyl sulfoxide and 4 mL of deionized water to prepare a mixed solvent, dissolve 1.30 g of triethylamine in the mixed solvent to obtain a triethylamine solution, transfer the triethylamine solution to a constant pressure dropping funnel, add it dropwise to reactant 2 at a rate of 1 d / 2 s, stir the reaction at 45 °C for 48 h to obtain reactant 3;

[0063] (5) Pour reactant 3 into a polytetrafluoroethylene mold and dry it in an oven at 45°C under normal pressure. Then rinse it with acetone and dry it again to obtain vinyltrimethylsilane modified gelatin.

[0064] Infrared spectroscopy was performed on the gelatin before modification, vinyltrimethylsilane, and the vinyltrimethylsilane-modified gelatin obtained above. The results are as follows: Figure 1 As shown; by Figure 1 It can be seen that vinyltrimethylsilane modified gelatin at 1140 cm⁻¹ -1 The characteristic Si-O peaks appeared at 2960 cm⁻¹, and vinyltrimethylsilane showed a peak at 2960 cm⁻¹. -1 The stretching vibration of the -CH3 bond disappears; vinyltrimethylsilane-modified gelatin also shows a vibration at 3110 cm⁻¹. -1 Amide II band at NH bond bending vibration, 3020 cm -1 The stretching vibration peak of the CH bond in the olefin, 1590 cm⁻¹ -1 Amide II band at NH bond bending vibration, 1480 cm -1 Amide III band of CN bond stretching vibration at 1360 cm⁻¹ -1 1300cm -1 The peaks at 979 cm⁻¹ represent the symmetric stretching vibration of the methyl CH bond and the bending vibration of the CH bond.

[0065] The gelatin before modification, vinyltrimethylsilane, and the vinyltrimethylsilane-modified gelatin obtained above were subjected to 1H NMR spectroscopy, and the results are as follows: Figure 2 As shown; by Figure 2 It can be seen that, compared with the gelatin before modification, the vinyltrimethylsilane modified gelatin showed a new chemical shift. The a and c peaks near 2.75 ppm and 2.4 ppm are both proton peaks of methyl groups, and the b peak near 2.6 ppm is a proton peak of vinyl groups. This indicates that the vinyltrimethylsilane modified gelatin has been successfully synthesized.

[0066] Experimental Example 1:

[0067] Mechanical property analysis of vinyltrimethylsilane modified gelatin

[0068] Weigh 0.05 g each of the gelatin before modification (gelatin) and the vinyltrimethylsilane modified gelatin (silanized modified gelatin) prepared in Example 1 and place them in 1 mL of deionized water. Prepare a 5% wt gelatin solution at 45 °C and a stirring speed of 200 rad / s. Place each gelatin solution at 4 °C to accelerate the formation of gelatin gel.

[0069] After the gelatin gel is formed, the gel is made into a dumbbell shape with a diameter of 60 mm and a length of 10 mm. The tensile strength, Young's modulus and elongation at break of the gelatin are tested on an electronic universal tensile testing machine at 25°C. The weight sensor of the electronic universal tensile testing machine is 200 N and the crosshead speed is 5 mm / min.

[0070] The formula for calculating tensile strength is: P = F / S; where: P is the tensile strength (N·mm). 2 F is the load force (N) at which the gel breaks, and S is the cross-sectional area of ​​the gel (mm²). 2 );

[0071] The formula for calculating Young's modulus is: E = σ / ε; where: E is Young's modulus (kPa), σ is tensile stress (kPa), and ε is tensile strain (%).

[0072] The formula for calculating elongation at break is: e = (L a -L0) / L0; where: e is the elongation at break (%), L0 is the initial length of the gel, L a This refers to the elongation at the moment the gel breaks.

[0073] The stress-strain curves of each gelatin sample are as follows: Figure 3 As shown in Table 1, the tensile strength, Young's modulus, and elongation at break are as follows:

[0074] Table 1. Test results of tensile strength, Young's modulus, and elongation at break for each gelatin sample

[0075] Sample Name <![CDATA[P(N·mm 2 )]]> E (kPa) e(％) gelatin before modification 36.2 9.4 26.5 Vinyltrimethylsilane modified gelatin 43.2 10.8 33.2

[0076] Depend on Figure 3 As shown in Table 1, the tensile strength of vinyltrimethylsilane-modified gelatin increased by 19.3% compared to the gelatin before modification, the Young's modulus increased by 14.8% compared to the gelatin before modification, and the elongation at break increased by 25.2% compared to the gelatin before modification. This indicates that vinyltrimethylsilane-modified gelatin has better resistance to external forces, and its toughness and rigidity have been correspondingly improved.

[0077] Experimental Example 2:

[0078] Hydrophobicity analysis of vinyltrimethylsilane modified gelatin

[0079] 0.05 g each of the unmodified gelatin and the vinyltrimethylsilane-modified gelatin prepared in Example 1 were weighed and placed in 1 mL of deionized water. A 5% wt gelatin solution was prepared at 45°C and a stirring speed of 200 rad / s. The gelatin solution was then dropped onto isolated pig skin using a dropper and allowed to air dry naturally, forming a smooth film, which was the gelatin sample. The contact angle (θ) of the gelatin sample was measured using a contact angle meter. The experimental results are shown in Table 2 below.

[0080] Table 2. Contact angle measurement results for each gelatin sample

[0081]

[0082]

[0083] As shown in Table 2, the contact angle of the vinyltrimethylsilane-modified gelatin increased by 20° compared with that of the unmodified gelatin. This indicates that the vinyltrimethylsilane-modified gelatin has better hydrophobicity. The enhanced hydrophobicity of gelatin can make it more effective in peeling certain skin contact materials.

[0084] Experimental Example 3:

[0085] Biocompatibility test of vinyltrimethylsilane modified gelatin

[0086] Cytotoxicity assays simulate the growth environment of organisms in vitro, allowing for the screening of cytotoxicity in samples within a short time. This is an important foundation for conducting other cell experiments. The Cell Counting Kit-8 (CCK-8) reagent can be used for rapid and convenient identification and analysis of the toxic effects of substances. Its mechanism of action is as follows: It contains WTS-8 [2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene)-2H-tetrazole monosodium salt], whose molecular structure contains multiple nitro (-NO2) and aryl sulfonic acid (-SO3H) groups. These groups have strong light absorption and undergo photolysis to produce free radicals or ions, which further undergo redox reactions. Under the action of 1-Methoxy PMS, they are reduced by intracellular dehydrogenases to a yellow formazan product. The amount of product is directly proportional to the survival rate. The yellow formazan product is characterized by high water solubility and a specific absorption at 450 nm. The cytotoxicity of the analyte can be determined by detecting its absorbance.

[0087] This experiment used human skin fibroblasts as a cell model and vinyltrimethylsilane-modified gelatin as the test sample. The CCK-8 assay was performed, and the specific steps are as follows:

[0088] (1) Select human skin fibroblasts of suitable density, digest them with trypsin to prepare a cell suspension, and seed them in 96-well plates. Control the volume of the suspension in each well to be 100 μL, and the number of cells in the suspension to be 6–8 × 10⁶ cells / well. 3 One sample group was prepared; a blank control group was set up at the same time. No cells were added to the blank control group, and only the same volume of cell suspension was added to each well; the 96-well plate was incubated in a 37°C, 5% CO2 incubator for 24 hours.

[0089] (2) After the cells in the 96-well plate from step (1) have adhered firmly, vinyltrimethylsilane-modified gelatin is added to the sample group and the blank control group, respectively, so that the final concentration of vinyltrimethylsilane-modified gelatin in each well is 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL and 100 μg / mL, respectively, and three replicates are set for each concentration; the 96-well plate is incubated at 37℃ in a 5% CO2 incubator for 24 h.

[0090] (3) After incubation, remove the 96-well plate from the incubator, add 10 μL of CCK-8 detection solution to each well, and continue incubation at 37°C and 5% CO2 for 3 h; after incubation, measure the absorbance of the liquid in the well plate at 450 nm.

[0091] (4) Results Analysis: Cell viability was calculated using the measured absorbance, and the calculation formula is as follows:

[0092] Cell viability = A x / A0×100%; where A x A0 represents the absorbance of the blank control group at 450 nm, and A0 represents the absorbance of the sample group at 450 nm.

[0093] The results of cell viability detection at different concentration gradients are as follows: Figure 4 As shown; by Figure 4 It can be seen that the survival rate of human skin fibroblasts under vinyltrimethylsilane modified gelatin is close to 100%, which indicates that vinyltrimethylsilane modified gelatin products have reached the implantable level.

[0094] Experiment Example 4:

[0095] Antioxidant experiment of vinyltrimethylsilane modified gelatin

[0096] I. Hydroxyl radical scavenging ability

[0097] Hydroxyl radicals are generated using the Fenton reaction: H₂O₂ + Fe 2+ =OH - +Fe 3+ +H₂O; Adding salicylic acid to the reaction system allows the hydroxyl radicals generated in the Fenton reaction to react with the salicylic acid, producing 2,3-dihydroxybenzoic acid, which has a specific absorption at 510 nm. Adding an analyte with hydroxyl radical scavenging capabilities to the reaction system reduces the generation of hydroxyl radicals, thereby correspondingly reducing the amount of 2,3-dihydroxybenzoic acid produced. This experimental example uses a fixed reaction time method to measure absorbance at 510 nm to determine the scavenging effect of vinyltrimethylsilane-modified gelatin on hydroxyl radicals. The specific steps are as follows:

[0098] First, add 7 mL each of 100 μg / mL modified pre-gelatin (gelatin) and vinyltrimethylsilane modified gelatin (silanized modified gelatin) to different test tubes. Then, add 1 mL each of 9 mmol / L salicylic acid, 9 mmol / L FeSO4, and 8.8 mmol / L H2O2 to each test tube sequentially. React at a constant temperature of 37℃ for 30 min to obtain the reaction product. Measure the absorbance of the reaction product at 510 nm using a UV-Vis spectrophotometer, and calculate the hydroxyl radical scavenging rate using the following formula:

[0099] Hydroxyl radical scavenging rate = [1-(A x -A x0 ) / A0]×100%; Where: A x A represents the absorbance at 510 nm of the reaction system containing gelatin sample, salicylic acid, FeSO4, and H2O2. x0 A0 represents the absorbance of the reaction system containing gelatin sample, salicylic acid, FeSO4, and deionized water at 510 nm.

[0100] The detection results of hydroxyl radical scavenging rate are as follows: Figure 5 As shown; by Figure 5 It was found that the hydroxyl radical scavenging rates of the unmodified gelatin with a concentration of 12 mg / mL and the vinyltrimethylsilane-modified gelatin reached 94.3% and 97.1%, respectively, indicating that the vinyltrimethylsilane-modified gelatin has a more prominent scavenging effect on hydroxyl radicals.

[0101] II. DPPH free radical scavenging ability

[0102] The principle of the DPPH free radical scavenging method: DPPH is a free radical that is not easily altered. It appears deep purple in methanol or ethanol solutions and has a maximum absorption peak at 520 nm. The ability to scavenge DPPH is essentially a test of the antioxidant capacity of the sample: antioxidants can donate H+, causing DPPH to transform into DPPH-H, resulting in the solution color disappearing or lightening. The antioxidant capacity of the sample can be analyzed by measuring its absorbance. The ability of the analyte to scavenge DPPH free radicals can be evaluated by the change in absorbance of the DPPH free radicals before and after its addition; a greater change in absorbance indicates a stronger antioxidant capacity.

[0103] This experiment was conducted in 96-well plates, with sample group 1, sample group 2, and a control group. Each group had three replicate wells. Sample group 1 consisted of gelatin (before modification) dissolved in 180 μL of distilled water, with 90 μL of 0.1 mM DPPH solution added to achieve final gelatin concentrations of 2 mg / mL, 4 mg / mL, 6 mg / mL, 8 mg / mL, 10 mg / mL, and 12 mg / mL. Sample group 2 consisted of vinyltrimethylsilane-modified gelatin (silanized modified gelatin), with the same conditions as sample group 1. The control group used 180 μL of distilled water and 90 μL of 0.1 mM DPPH solution. After mixing all components, the 96-well plates were incubated in a shaker in the dark for 15 min. The OD value at 520 nm was measured using a microplate reader. 520 .

[0104] The formula for calculating DPPH free radical scavenging rate is: DPPH free radical scavenging rate = [(A0 - A...]] x [(A0) / A0]×100%; where A0 represents the absorbance OD of the control group. 520 A x The absorbance OD of the sample group 520 .

[0105] DPPH free radical scavenging rate test results are as follows Figure 6 As shown; by Figure 6 It can be seen that at a concentration of 12 mg / mL, the scavenging rates of unmodified gelatin and vinyltrimethylsilane-modified gelatin for DPPH free radicals reached 53.7% and 80.4%, respectively, and the scavenging rate was higher at higher concentrations, indicating that the scavenging ability of vinyltrimethylsilane-modified gelatin for DPPH free radicals was significantly improved.

[0106] III. Superoxide radical scavenging ability

[0107] Under weakly alkaline conditions, pyrogallol is readily auto-oxidized, and the intermediate products in the auto-oxidation process are colored and have a specific absorption peak at 320 nm. Therefore, the rate of pyrogallol auto-oxidation can be assessed by measuring changes in absorbance. If the analyte has antioxidant capacity, the yield of the readily auto-oxidized intermediate products of pyrogallol will decrease, causing a change in the absorbance of the solution. This principle can be used to evaluate the antioxidant capacity of the analyte.

[0108] This experiment used unmodified gelatin (gelatin) and vinyltrimethylsilane-modified gelatin (silanized modified gelatin) as samples to evaluate their antioxidant capacity. The specific operation steps are as follows:

[0109] (1) Zeroing solution: Take 600 μL of 0.1M Tris-HCl buffer (pH=8.2) and 600 μL of distilled water and place them in a centrifuge tube. Mix well and place in a 25℃ water bath for 20 min.

[0110] (2) Preparation of solution I: Mix 600 μL of 0.1 M Tris-HCl buffer solution (pH = 8.2) with 580 μL of distilled water, and add the sample to achieve final concentrations of 2 mg / mL, 4 mg / mL, 6 mg / mL, 8 mg / mL, 10 mg / mL, and 12 mg / mL; the control group is 1200 μL of distilled water.

[0111] (3) Preparation of solution II: 20 μL of 5 mM pyrogallol solution;

[0112] (4) Under a water bath at 37℃, rapidly mix solution I and solution II until homogeneous, and let stand for 10 min to obtain the reaction solution; measure the absorbance value (OD) of the reaction solution at 325 nm. 325 During the measurement process, the first time was recorded as 0 min, and a second record was made after a 5-minute interval. The superoxide radical scavenging rate of the sample was then calculated.

[0113] The formula for calculating the superoxide radical scavenging rate is: Superoxide radical scavenging rate = [(ΔA0 - ΔA] / (ΔA)] x [(ΔA0) / ΔA0]×100%; where ΔA0 represents the absorbance difference between the control group and the control group; ΔA x This indicates the difference in absorbance after the sample is added.

[0114] The results of the superoxide radical scavenging rate test are as follows: Figure 7 As shown; by Figure 7 It can be seen that the scavenging rates of superoxide radicals by pre-modified gelatin and vinyltrimethylsilane-modified gelatin with a concentration of 12 mg / mL reached 70.4% and 91.5%, respectively, and the scavenging rate was higher with higher concentration, which indicates that the scavenging ability of vinyltrimethylsilane-modified gelatin for superoxide radicals was also greatly improved.

[0115] Experimental Example 5:

[0116] Cell scratch assay of vinyltrimethylsilane modified gelatin

[0117] The cell scratch assay is an experimental technique that studies cell migration, repair capabilities, and cell-cell interactions by scratching a cell monolayer and periodically capturing images using a time-lapse microscope. The basic principle is that a blank area, called a "scratch," is artificially created on a fused monolayer of cells. Cells at the edge of the scratch gradually move into the blank area, causing the "scratch" to heal. Images are captured periodically during cell migration, and by measuring the distance between scratches at different time points and calculating the difference, the cell's migration ability can be assessed. Because the cell scratch assay resembles the in vitro wound healing process, it is also known as the wound healing assay. It can be used to observe the effects of exogenous factors such as drugs and genes on cell migration, repair, and interactions.

[0118] Exogenous sodium hyaluronate can promote the proliferation and differentiation of epidermal cells and has a certain repair effect on skin damage. It can promote skin cell growth and wound healing, and restore the damaged area to health. Therefore, it is widely used as a skin wound healing and regeneration aid, drug carrier, etc.

[0119] The specific steps of this experiment are as follows:

[0120] (1) Use a marker to draw horizontal lines evenly on the back of the 6-hole plate, with the horizontal lines spaced 0.5 to 1 cm apart, passing through the holes, with at least 5 lines passing through each hole;

[0121] (2) Prepare a cell suspension from Hacat cells (human immortalized epidermal cells) cultured to 80-90% confluence, using 5×10 5 The cells were added to the well plates and the well plates were placed in a 5% CO2, 37°C cell culture incubator for culture.

[0122] (3) When the cultured cells reach a confluence of more than 95%, use a 200μL pipette tip to make a straight scratch on the bottom of the well plate perpendicular to the horizontal line, and take a photo to record the scratch at 0h.

[0123] (4) Add 100 μg / mL of sample solution to the well plate respectively. The sample solutions are gelatin before modification (gelatin) solution, vinyltrimethylsilane modified gelatin (silanized modified gelatin) solution and sodium hyaluronate solution. The blank control group is the same volume of cell culture medium. After culturing for 24 h, take pictures to record the healing of cell scratches and calculate the cell migration rate.

[0124] The cell migration rate is calculated using the following formula: M = (S0 - S t ) / S0; where S0 represents the initial scratch area, S t This represents the area of ​​the scratch at time t.

[0125] Cell scratch images at 0h and 24h are as follows Figure 8 As shown, the calculated cell migration rate over 24 hours is as follows: Figure 9 As shown; by Figure 9 It was found that both the sample group and the control group exhibited cell migration behavior to varying degrees at 24 hours, with the migration rate of the sample group being higher than that of the blank control group. In terms of cell migration rate, the migration rates of vinyltrimethylsilane-modified gelatin, unmodified gelatin, sodium hyaluronate, and the blank control group were 65.4%, 24.1%, 36.8%, and 18.6%, respectively. This indicates that vinyltrimethylsilane-modified gelatin can promote cell migration more quickly than unmodified gelatin, and its effect surpasses that of sodium hyaluronate. It holds promise for application in wound repair technology to promote wound healing.

[0126] Example 2:

[0127] Application of vinyltrimethylsilane modified gelatin in skin care products

[0128] I. Preparation of Moisturizing Face Cream:

[0129] This embodiment applies vinyltrimethylsilane-modified gelatin to a moisturizing face cream to study the efficacy of vinyltrimethylsilane-modified gelatin in skincare products.

[0130] First, four different formulas were used to prepare moisturizing face creams, and the specific components are shown in Table 3 below:

[0131] Table 3. Ingredients of Moisturizing Cream

[0132]

[0133] Prepare moisturizing face creams according to the four formulas in Table 3, with the specific steps as follows:

[0134] (1) Accurately weigh out phase A, disperse it evenly, and heat it to 80℃ to obtain component 1;

[0135] (2) Accurately weigh phase C and heat it to 80℃ to obtain component 2;

[0136] (3) Under homogenization, add component 1 to component 2, keep the temperature at 75-80℃, and homogenize for 3 min;

[0137] (4) Add phase B, homogenize for 2 minutes, and then cool down while stirring;

[0138] (5) When the temperature drops to 65°C, add phase E, stir evenly, and continue to cool down while stirring.

[0139] (6) When the temperature drops to 45℃, add phase D and stir evenly, then add phase F (phase F is not added in formula 1) and continue stirring evenly to obtain a moisturizing cream.

[0140] II. Safety Properties Testing of Face Cream

[0141] (1) pH value evaluation:

[0142] The direct measurement method was used. First, at room temperature, an appropriate amount of face cream sample from formulas 1 to 4 was placed in a beaker for later use. After calibrating the pH meter, the electrode was rinsed with distilled water and dried with a paper towel. The electrode was slowly placed into the face cream sample. After the reading stabilized, the value was recorded accurately to 0.1. After recording the data, the electrode was cleaned and the measurement was continued. A total of 5 measurements were taken, and the average value was recorded.

[0143] After testing, all four formulas of the moisturizing face cream had a pH value of 6.5, which is suitable for human skin.

[0144] (2) Stability evaluation

[0145] ① Centrifugation experiment: At room temperature, samples from formulas 1-4 were placed in a high-speed centrifuge and centrifuged for 30 minutes at speeds of 500 rpm / min, 1000 rpm / min, 2000 rpm / min, and 3000 rpm / min, respectively. The blank group was not centrifuged. The experimental results for formula 3 face cream are as follows: Figure 10 As shown, the face creams prepared by formulas 1 to 4 did not exhibit any precipitation, stratification, discoloration, or significant changes in hardness after high-speed centrifugation, and were able to maintain a stable form.

[0146] ② Heat Resistance Test: Take two equal portions of samples from formulas 1-4. Place one portion at room temperature and the other in a 40℃ constant temperature chamber for 10 days. After 10 days, remove the sample and place it at room temperature for at least 8 hours. Then compare the sample with the one at room temperature and observe whether the sample in the high-temperature environment shows any changes such as discoloration, precipitation, layering, or hardness. The experimental results for formula 3 face cream are as follows: Figure 11 As shown in Figure A, the sample on the left is at room temperature.

[0147] ③ Cold Resistance Test: Take two equal portions of samples from formulas 1-4. Place one portion at room temperature and the other in a -20℃ freezer for 10 days. After 10 days, remove the sample and allow it to stand at room temperature for at least 8 hours. Then compare the sample with the sample at room temperature and observe whether there are any changes in color, precipitation, layering, or hardness in the sample in the low-temperature environment. The experimental results for formula 3 face cream are as follows: Figure 11 As shown in Figure B, the sample on the left is at room temperature.

[0148] ④ Alternating Hot and Cold Test: Take two equal portions of samples from formulas 1-4. Place one portion at room temperature, and the other portion successively in a 40℃ constant temperature incubator and a 20℃ refrigerator, alternating between hot and cold for 24 hours, for a total of 10 days. Afterward, remove the sample and place it at room temperature for at least 8 hours. Then compare it with the sample at room temperature and observe whether there are any changes in color, precipitation, layering, or hardness in the sample under alternating hot and cold conditions. The experimental results for formula 3 face cream are as follows: Figure 11 As shown in Figure C, the left side represents the sample at room temperature.

[0149] The face creams prepared by formulas 1 to 4 above did not exhibit any precipitation, layering, discoloration, or significant changes in hardness after undergoing cold resistance, heat resistance, and alternating hot and cold tests. They were able to maintain a stable form, indicating that the face creams have good stability.

[0150] III. Safety Evaluation of Face Cream

[0151] There are many types of skincare products with complex and diverse ingredients. In order to ensure the safety of human use and avoid skin allergies or other reactions, it is necessary to evaluate the safety of the products. Currently, two commonly used methods are human trial experiments and patch tests.

[0152] Patch testing is the most commonly used evaluation method for assessing the safety of cosmetic ingredients and cosmetics. This experiment used a human patch test to evaluate the safety of a face cream. First, 15 subjects were selected. All participants were required to participate voluntarily, have no history of skin allergies, be in good health, and not have participated in other skincare efficacy evaluations in the past three months. Then, an appropriate amount of moisturizing face cream was placed in a standard patch test chamber. The patch, rich in the test substance, was slowly applied to the inside of the subject's arm for 12 hours to maintain product efficacy release. After the test, the patch was removed, and changes in the skin on the inside of the subject's arm were observed at 30 minutes (b), 12 hours (c), and 24 hours (d). The results are as follows: Figure 12 As shown in Table 4; finally, the results of the skin occlusion patch test were observed and recorded according to the scoring criteria in Table 4, and the results are shown in Table 5:

[0153] Table 4. Patch Test Scoring Criteria

[0154] Scoring Division Level Classification Reaction phenomenon 0 － negative reaction 1 ± Only faint erythema 2 + Weak positive reaction: infiltration, erythema 3 ++ Strong positive reaction: erythema, infiltration, edema, extending beyond the test area 4 +++ Extremely strong positive reaction: obvious erythema, severe infiltration, extending beyond the test area

[0155] Table 5. Patch Test Results

[0156] Depend on Figure 12 As shown in Table 8, none of the 15 subjects experienced adverse reactions, indicating that the moisturizing creams prepared by formulas 1 to 4 all meet the safety requirements.

[0157] IV. Evaluation of the efficacy of face cream

[0158] The face creams prepared according to formulas 1-4 were applied to the skin of the subjects to further test the efficacy of the face creams. The specific steps are as follows:

[0159] (1) Skin moisture content test: In this operation, the Coreometer CM825 skin moisture content test probe is used for testing. The probe test principle is based on the capacitance method. The specific method is as follows: First, disinfect the instrument probe with 75% alcohol and adjust the equipment. Open the software, place the probe vertically downward and gently press it on the test site of the subject. Take 3 points in the same area and finally take the average value, which is the stratum corneum moisture content. The larger the value, the higher the skin moisture content.

[0160] Moisturizing creams have a moisturizing effect on the skin, such as Figure 13 As shown; by Figure 13 It was found that the moisture content of the stratum corneum increased by -2.1%, 30.2%, 40.7%, and 51.3% at 6 hours compared to 0 hours, respectively. This indicates that the addition of vinyltrimethylsilane-modified gelatin and tripeptide can increase the moisture content of the stratum corneum, and the modified gelatin has excellent moisturizing effect.

[0161] (2) Skin Elasticity Test: Skin elasticity is an important indicator of skin condition, in which the content and arrangement of collagen and elastin fibers play a key role. During aging, the loss of ring-shaped elastin fibers and vertically arranged filaments leads to skin sagging, wrinkles, and reduced elasticity. By testing skin elasticity, the degree of skin aging can be reflected to some extent. This procedure uses the Cutometer MPA580 instrument probe based on the principles of suction and stretching. By analyzing the relationship between the length of skin stretching and time, parameters representing skin elasticity indicators are obtained, and the skin elasticity values ​​R2 and R5 are recorded. The larger the R2 and R5 values, the better the skin elasticity.

[0162] Moisturizing creams have effects on skin elasticity such as Figure 14 The diagrams A through D are shown in the image, where A, B, C, and D represent formula 1, formula 2, formula 3, and formula 4, respectively. Figure 14 It can be seen that when using face creams of formulas 3 and 4, the R2 and R5 values ​​both showed an upward trend compared to h0, and the differences were significant (**P<0.01); the R2 values ​​increased by 13.6% and 16.4%, respectively, and the R5 values ​​increased by 11.8% and 12.3%, respectively; this indicates that the addition of vinyltrimethylsilane modified gelatin can significantly improve skin elasticity.

[0163] (3) Skin roughness test: Roughness measures the degree of skin dryness. The skin surface texture test system is used to take images of the skin surface and the skin surface analysis software SELS is used to analyze the gray values ​​of the skin surface to obtain the test parameter Ser. The specific method is as follows: establish a test project, use VisioScan VC 20plus to take pictures of the test area, record the skin roughness data, and export the captured images; the smaller the Ser value, the lower the skin roughness.

[0164] Experimental results on how moisturizing face creams improve skin roughness include... Figure 15 , 16 As shown; by Figure 15 It was found that after using the face creams of formulas 3 and 4 for 28 days, skin roughness decreased significantly; Figure 16 It can be seen that after using the face cream 14D of formula 3 and formula 4, the roughness of the face decreased by 9.9% and 13.4% respectively; this shows that the addition of vinyltrimethylsilane modified gelatin can significantly improve skin roughness and effectively reduce wrinkles.

[0165] Example 2:

[0166] Unlike Example 1, in Example 2, the weight ratio of gelatin, tetrabutylammonium iodide, vinyltrimethylsilane, and triethylamine is 30:1:5:1; other steps are the same as in Example 1; finally, vinyltrimethylsilane modified gelatin is obtained.

[0167] Example 3:

[0168] Unlike Example 1, in step (1) of Example 3, a 4% wt gelatin solution was prepared, and after standing at room temperature for 1 hour, the three-necked flask containing the gelatin solution was placed in a 45°C water bath and stirred for 10 minutes to adjust the pH to 10; the other steps were the same as in Example 1; finally, vinyltrimethylsilane modified gelatin was obtained.

[0169] Example 4:

[0170] Unlike Example 1, in step (4) of Example 4, 5 mL of dimethyl sulfoxide and 5 mL of deionized water were prepared into a mixed solvent, and 1.30 g of triethylamine was dissolved in the mixed solvent to obtain a triethylamine solution. The triethylamine solution was transferred to a constant pressure dropping funnel and added dropwise to reactant 2 at a rate of 1 d / 2 s. The mixture was stirred at 46 °C for 45 h to obtain reactant 3. Other steps were the same as in Example 1. Finally, vinyltrimethylsilane modified gelatin was obtained.

[0171] In summary, this invention uses vinyltrimethylsilane to modify gelatin. In addition to having strong hydrophobicity, antioxidant properties, and moisturizing properties, the modified gelatin also has better resistance to external forces, and can promote cell migration and accelerate the healing of skin wounds.

Claims

1. A vinyltrimethylsilane-modified gelatin, characterized in that, The vinyltrimethylsilane-modified gelatin has a contact angle of 90-98°, a scavenging rate of 90-98.6% for hydroxyl radicals, a scavenging rate of 73-80.4% for DPPH radicals, a scavenging rate of 85-91.5% for superoxide radicals, and a cell migration rate of 60-65.4% for epidermal cells. The tensile strength of the vinyltrimethylsilane-modified gelatin is 19.3% higher than that of the unmodified gelatin, the Young's modulus is 14.8% higher, and the elongation at break is 25.2% higher. The preparation method of the vinyltrimethylsilane modified gelatin specifically includes the following steps: (1) Prepare a 3-5%wt gelatin solution, let it stand at room temperature, put the gelatin solution into a 42-48℃ water bath and stir for 10-15 minutes, and adjust the pH to 9-10; (2) Tetrabutylammonium iodide was added to the gelatin solution in step (1) under oxygen-free conditions to obtain reactant 1; (3) Dissolve vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, add vinyltrimethylsilane solution to reactant 1, mix thoroughly to obtain reactant 2; (4) Prepare a mixed solvent by mixing dimethyl sulfoxide and water in a ratio of 5~7:4~5 (v / v), dissolve triethylamine in the mixed solvent to obtain a triethylamine solution, add the triethylamine solution to reactant 2, and stir the reaction at 42~48℃ for 40~50 h to obtain reactant 3; (5) Pour reactant 3 into a mold and dry it in an oven at 40~45℃ under normal pressure. Then rinse it with acetone and dry it again to obtain vinyltrimethylsilane modified gelatin.

2. The vinyltrimethylsilane-modified gelatin as described in claim 1, characterized in that, The infrared spectrum of the vinyltrimethylsilane-modified gelatin is at 1140 cm⁻¹. -1 The Si-O characteristic peaks appeared, as well as at 3110 cm⁻¹. -1 Amide II band at NH bond bending vibration, 3020 cm -1 The stretching vibration peak of the CH bond in the olefin, 1590 cm⁻¹ -1 Amide II band at NH bond bending vibration, 1480 cm -1 Amide III band of CN bond stretching vibration at 1360 cm⁻¹ -1 1300cm -1 The symmetric stretching vibration peak of the methyl CH bond, 979 cm⁻¹ -1 The bending vibration peak of the CH bond.

3. The vinyltrimethylsilane-modified gelatin as described in claim 1, characterized in that, The standing time at room temperature in step (1) is more than 1 hour.

4. The vinyltrimethylsilane-modified gelatin as described in claim 1, characterized in that, The method for isolating oxygen in step (2) is as follows: nitrogen gas is continuously introduced into the gelatin solution of step (1) for 10 minutes, then tetrabutylammonium iodide is added, and nitrogen gas is introduced again for 30 minutes.

5. The vinyltrimethylsilane-modified gelatin as described in claim 1, characterized in that, The weight ratio of gelatin, tetrabutylammonium iodide, vinyltrimethylsilane, and triethylamine is 30-40:1:5:

1.

6. The use of the vinyltrimethylsilane modified gelatin according to claim 1 in the preparation of skin care products.

7. The application as described in claim 6, characterized in that, The skincare products include toners, lotions, moisturizing creams, hand creams, foot creams, bath products, shampoos, and hair care products. The dosage forms of the skincare products include liquids, lotions, creams, gels, and masks.

8. The application of vinyltrimethylsilane, characterized in that, It is used to improve the tensile strength of gelatin, and the application method is as follows: (1) Prepare a 3-5%wt gelatin solution, let it stand at room temperature, put the gelatin solution into a 42-48℃ water bath and stir for 10-15 minutes, and adjust the pH to 9-10; (2) Tetrabutylammonium iodide was added to the gelatin solution in step (1) under oxygen-free conditions to obtain reactant 1; (3) Dissolve vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, add vinyltrimethylsilane solution to reactant 1, mix thoroughly to obtain reactant 2; (4) Prepare a mixed solvent by mixing dimethyl sulfoxide and water in a ratio of 5~7:4~5 (v / v), dissolve triethylamine in the mixed solvent to obtain a triethylamine solution, add the triethylamine solution to reactant 2, and stir the reaction at 42~48℃ for 40~50 h to obtain reactant 3; (5) Pour reactant 3 into a mold and dry it in an oven at 40~45℃ under normal pressure. Then rinse it with acetone and dry it again to obtain vinyltrimethylsilane modified gelatin. The vinyltrimethylsilane-modified gelatin has a contact angle of 90-98°, a scavenging rate of 90-98.6% for hydroxyl radicals, a scavenging rate of 73-80.4% for DPPH radicals, a scavenging rate of 85-91.5% for superoxide radicals, and a cell migration rate of 60-65.4% for epidermal cells. The tensile strength of the vinyltrimethylsilane-modified gelatin is 19.3% higher than that of the gelatin before modification, the Young's modulus is 14.8% higher than that of the gelatin before modification, and the elongation at break is 25.2% higher than that of the gelatin before modification.

9. The application of vinyltrimethylsilane, characterized in that, It is used to improve the antioxidant properties of gelatin. The application method is as follows: (1) Prepare a 3-5%wt gelatin solution, let it stand at room temperature, put the gelatin solution into a 42-48℃ water bath and stir for 10-15 minutes, and adjust the pH to 9-10; (2) Tetrabutylammonium iodide was added to the gelatin solution in step (1) under oxygen-free conditions to obtain reactant 1; (3) Dissolve vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, add vinyltrimethylsilane solution to reactant 1, mix thoroughly to obtain reactant 2; (4) Prepare a mixed solvent by mixing dimethyl sulfoxide and water in a ratio of 5~7:4~5 (v / v), dissolve triethylamine in the mixed solvent to obtain a triethylamine solution, add the triethylamine solution to reactant 2, and stir the reaction at 42~48℃ for 40~50 h to obtain reactant 3; (5) Pour reactant 3 into a mold and dry it in an oven at 40~45℃ under normal pressure. Then rinse it with acetone and dry it again to obtain vinyltrimethylsilane modified gelatin. The vinyltrimethylsilane-modified gelatin has a contact angle of 90-98°, a scavenging rate of 90-98.6% for hydroxyl radicals, a scavenging rate of 73-80.4% for DPPH radicals, a scavenging rate of 85-91.5% for superoxide radicals, and a cell migration rate of 60-65.4% for epidermal cells. The tensile strength of the vinyltrimethylsilane-modified gelatin is 19.3% higher than that of the gelatin before modification, the Young's modulus is 14.8% higher than that of the gelatin before modification, and the elongation at break is 25.2% higher than that of the gelatin before modification.

10. The application of vinyltrimethylsilane, characterized in that, It is used to improve the hydrophobicity of gelatin, and the application method is as follows: (1) Prepare a 3-5%wt gelatin solution, let it stand at room temperature, put the gelatin solution into a 42-48℃ water bath and stir for 10-15 minutes, and adjust the pH to 9-10; (2) Tetrabutylammonium iodide was added to the gelatin solution in step (1) under oxygen-free conditions to obtain reactant 1; (3) Dissolve vinyltrimethylsilane in dimethyl sulfoxide to obtain vinyltrimethylsilane solution, add vinyltrimethylsilane solution to reactant 1, mix thoroughly to obtain reactant 2; (4) Prepare a mixed solvent by mixing dimethyl sulfoxide and water in a ratio of 5~7:4~5 (v / v), dissolve triethylamine in the mixed solvent to obtain a triethylamine solution, add the triethylamine solution to reactant 2, and stir the reaction at 42~48℃ for 40~50 h to obtain reactant 3; (5) Pour reactant 3 into a mold and dry it in an oven at 40~45℃ under normal pressure. Then rinse it with acetone and dry it again to obtain vinyltrimethylsilane modified gelatin. The vinyltrimethylsilane-modified gelatin has a contact angle of 90-98°, a scavenging rate of 90-98.6% for hydroxyl radicals, a scavenging rate of 73-80.4% for DPPH radicals, a scavenging rate of 85-91.5% for superoxide radicals, and a cell migration rate of 60-65.4% for epidermal cells. The tensile strength of the vinyltrimethylsilane-modified gelatin is 19.3% higher than that of the gelatin before modification, the Young's modulus is 14.8% higher than that of the gelatin before modification, and the elongation at break is 25.2% higher than that of the gelatin before modification.

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