Organ-like 3D printer temperature control system

Abstract

The organ-like 3D printer temperature control system comprises a first liquid guide assembly, a first driving assembly, a pore plate and a first temperature control assembly. The first liquid guiding assembly is provided with a first liquid guiding end used for guiding out biological ink. The first driving assembly is connected to the first liquid guiding assembly and used for driving the first liquid guiding assembly to move. The pore plate is arranged below the first liquid guide assembly, the pore plate comprises an upper surface wall and a lower surface wall, the upper surface wall is concavely provided with a plurality of accommodating grooves, and each accommodating groove is used for accommodating the biological ink guided out by the first liquid guide end. The first temperature control assembly is attached to the lower surface wall and used for adjusting the temperature value of the pore plate to a first preset range. According to the invention, the accommodating grooves are integrated on the pore plate, and the temperature of the solution in each accommodating groove can be mutually conducted through the pore plate, so that the temperature of each accommodating groove can tend to be average, and operations such as large-batch printing and culturing of the organoids can be more efficiently and accurately carried out.

Description

Technical Field

[0001] The present invention relates to the field of organoid and cell culture technology, and in particular to a temperature control system for an organoid 3D printer. Background Art

[0002] In today's industrial production and technical applications, containers, as a basic component, are widely used in many fields. However, with the continuous advancement of technology and the increase in application requirements, the container design in the existing technology has gradually exposed some shortcomings.

[0003] Specifically, existing container designs are often independent entities. Although this design is simple and easy to manufacture, it has many problems in actual use. In particular, in application scenarios where heating or reaction is required, independent containers are often difficult to achieve uniform heating, resulting in low reaction efficiency and may even cause safety issues.

[0004] For example, in chemical laboratories or industrial production lines, it is often necessary to heat the substances in containers to trigger chemical reactions. However, due to the independence of existing containers, it is difficult to transfer heat evenly to each container, resulting in some containers being overheated while others are underheated. This uneven heating condition not only affects the efficiency of the reaction, but may also trigger unnecessary side reactions and even cause safety hazards. Summary of the invention

[0005] The main purpose of the present invention is to propose a temperature control system for an organoid 3D printer, which can more efficiently and accurately perform large-scale organoid manufacturing, reaction testing and other operations.

[0006] To achieve the above objectives, some embodiments of the present invention provide a temperature control system for an organoid 3D printer, comprising: A first liquid guiding component, comprising a first liquid guiding end for guiding out the biological ink; A first driving component, connected to the first liquid guiding component, and used to drive the first liquid guiding component to move; An orifice plate is disposed below the first liquid guiding component, the orifice plate comprises an upper surface wall and a lower surface wall, the upper surface wall is concavely provided with a plurality of receiving grooves, each receiving groove is used to receive the biological ink guided out by the first liquid guiding end; The first temperature control component is attached to the lower surface wall, and the first temperature control component is used to adjust the temperature value of the orifice plate to a first preset range.

[0007] In some embodiments, the temperature control system of the organoid 3D printer includes a placement platform, and the orifice plate and the first temperature control component are respectively disposed on opposite sides of the placement platform; Among them, the placement platform is recessed to form a first recessed portion, which is used to accommodate the orifice plate. The side of the placement platform away from the first recessed portion is recessed in the opposite direction of the recessed direction of the first recessed portion to form a second recessed portion, which is used to accommodate the first temperature control component.

[0008] In some embodiments, the first temperature control assembly includes a first temperature control device and a second temperature control device, the first temperature control device is fixed to the placement platform, and the first temperature control device is attached to the lower surface wall of the orifice plate to adjust the temperature of the orifice plate; The second temperature control device is movably connected to the placement platform to adjust the local temperature of the orifice plate.

[0009] In some embodiments, the first temperature control component includes a driving device connected to the second temperature control device, and the driving device is configured to drive the second temperature control device to move relative to the orifice plate to adjust the temperature of the area of ​​the orifice plate containing the biological ink.

[0010] In some embodiments, the placement platform includes a plurality of upwardly protruding stoppers, and the plurality of stoppers all abut against the edge of the well plate.

[0011] In some embodiments, the temperature control system of the organoid 3D printer further comprises a temperature probe, which is connected to the placement platform and is located on a side of the placement platform away from the well plate.

[0012] In some embodiments, the temperature control system of the organoid 3D printer includes a second temperature control component, which is connected to the first liquid conducting component, and the second temperature control component is used to adjust the temperature of the biological ink exported by the first liquid conducting component to within a second preset range.

[0013] In some embodiments, the temperature control system of the organoid 3D printer includes a first driving component, the first driving component is connected to the first liquid guiding component, and the first driving component is used to drive the first liquid guiding component to move along a first preset path; Among them, the first driving component is configured to first move the liquid guiding component toward the direction close to the solution to be treated until the biological ink discharged from the first liquid guiding end adheres to the solution to be treated, and then move the first liquid guiding component away from the solution to be treated until the biological ink separates from the first liquid guiding end.

[0014] In some embodiments, the organoid 3D printer temperature control system also includes a second liquid conducting component and a second driving component, the second liquid conducting component has a second liquid conducting end for conducting culture fluid, the second driving component is connected to the second liquid conducting component, and the second driving component drives the second liquid conducting component to move along a second preset path to inject the culture fluid into the holding tank.

[0015] In some embodiments, the organoid 3D printer temperature control system also includes a shell component, the shell component has an inner chamber, and the inner chamber can seal and accommodate the first liquid guide component, the first drive component, the orifice plate and the first temperature control component.

[0016] According to the above embodiments, the beneficial effects of the present invention are: The temperature control system of the organoid 3D printer of the present invention comprises a first liquid guide component, a first drive component, a well plate and a first temperature control component. The first liquid guide component has a first liquid guide end for guiding out the biological ink, and the design of the first liquid guide end enables the biological ink to be evenly dripped into each receiving groove of the well plate. The first drive component is connected to the first liquid guide component, and its function is to drive the first liquid guide component to move precisely to ensure that the biological ink can be accurately dripped into the designated position. The well plate is arranged below the first liquid guide component, and the well plate is composed of an upper surface wall and a lower surface wall. A plurality of receiving grooves are recessed on the upper surface wall. The layout design of these receiving grooves is intended to maximize the space for cell growth while ensuring the uniformity of the distribution of the biological ink, thereby improving the culture efficiency of the temperature control system of the organoid 3D printer. The first temperature control component is installed close to the lower surface wall of the well plate to adjust the temperature value of the well plate to a first preset range suitable for cell growth. This structural design ensures that cells grow in a suitable temperature environment, thereby improving the efficiency and quality of biological reactions.

[0017] The present invention integrates the holding tanks on the orifice plate, and the holding tanks are connected by the orifice plate. The heat received by the orifice plate can be conducted between the holding tanks, or the temperature of the solution in each holding tank can be conducted and influenced by each other through the orifice plate. Therefore, the design of the present application can make the temperature of each holding tank tend to be average. Such a design is conducive to ensuring that the solution in each holding tank has the same environmental conditions (such as temperature), and can more efficiently and accurately perform large-scale organoid printing, reaction testing and other operations.

[0018] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0020] Figure 1 This is a schematic diagram of the structure of a temperature control system for an organoid 3D printer in one embodiment of the present invention; Figure 2 yes Figure 1 Schematic diagram of the explosion structure of the temperature control system of the organoid 3D printer; Figure 3 yes Figure 2 Enlarged view of point A in the middle; Figure 4 A schematic diagram of the structure of the temperature control system of the organoid 3D printer observed from another perspective in one embodiment of the present invention; Figure 5 yes Figure 4 Schematic diagram of the cross-sectional structure of the temperature control system of the organoid 3D printer cut by the aa plane; Figure 6 This is a schematic diagram of the structure of the temperature control system of the organoid 3D printer behind the hidden outer door panel in one embodiment of the present invention; Figure 7 yes Figure 6 Schematic diagram of the cross-sectional structure of the temperature control system of the organoid 3D printer cut by the bb plane; Figure 8 It is a partial structural schematic diagram of the first liquid guiding component and the second liquid guiding component in one embodiment of the present invention; Fig. 9 From another perspective Figure 8 A schematic diagram of the structure of the first liquid guiding component and the second liquid guiding component; Fig.10 is a schematic structural diagram of a culture platform unit in one embodiment of the present invention; Fig.11 yes Fig.10 Schematic diagram of the cross-sectional structure of the culture platform unit cut by the cc plane.

[0021] Description of Figure Numbers: Shell assembly 10; filter fan 101; high-efficiency filter 102; microscope display screen 103; outer door panel 104; cabinet rear end support 105; machine support unit 106; load-bearing foot cup 107; cabinet lower end support 108; The second liquid guide assembly 20; a vertical precision screw motor 201; a motor slider 202; a hydraulic block 203; a liquid injection pump 204; a liquid packaging shell 205; a liquid adding device fixture 206; a microscope 207; a microscope slide 208; Culture platform unit 30; well plate holder 301; placement platform 302; temperature probe 303; first temperature control device 304; second temperature control device 305; First liquid guide assembly 40; vertical precision screw motor 401; motor slider 402; sample spotting block 403; sample spotting injection pump 404; sample spotting package shell 405; sample spotting circulating water cooling generator 406; temperature probe 407; sample spotting device holder 408; A second temperature control component 50; A first temperature control component 60; A first drive assembly 70; A second drive assembly 80; Orifice plate 90; Receiving groove 910.

[0022] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back, etc.), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0025] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features limited to "first" and "second" may explicitly or implicitly include at least one of the features. In addition, if "and / or", "and / or" or "and / or" appears in the full text, its meaning includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in the field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0026] In today's industrial production and technological applications, the manufacturing process of organoids often involves temperature control, including temperature control during the extrusion process and temperature control in porous containers, so that they can be smoothly manufactured and formed.

[0027] Specifically, the preparation of organoid precursors often involves the mixing and extrusion of hydrogels such as matrigel and cells. Among them, the cells and the hydrogel Matrigel are always kept at a low temperature during the mixing process, so that they present a fluid state that is easy to extrude. After extrusion, the matrigel must be solidified and formed in the range of 35°C-45°C while maintaining the activity of the cells to promote the development and maturation of organoids. The current printing device temporarily lacks a separate temperature control module for the organoid manufacturing process. In particular, there is no corresponding design and device for the temperature control device of the overall ambient temperature and the stage part of the porous container during the extrusion process, which makes it impossible to form organoids during the manufacturing process and maintain the activity of cells in the preparation of organoid precursors after forming.

[0028] Reference below Figures 1 to 11 The temperature control system of the organoid 3D printer according to an embodiment of the present invention is described below. Figures 1 to 3 as well as Fig.10 and Fig.11 In some embodiments, the temperature control system of the organoid 3D printer includes a first liquid guide component 40, a first drive component 70, a well plate 90 and a first temperature control component 60. The first liquid guide component 40 has a first liquid guide end for guiding the biological ink, and the design of the first liquid guide end allows the biological ink to be uniformly dripped into each receiving groove 910 of the well plate 90. The first drive component 70 is connected to the first liquid guide component 40, and its function is to drive the first liquid guide component 40 to move accurately to ensure that the biological ink can be accurately dripped into the designated position. The well plate 90 is arranged below the first liquid guide component 40, and the well plate 90 is composed of an upper surface wall and a lower surface wall. A plurality of receiving grooves 910 are recessed on the upper surface wall. The layout design of these receiving grooves 910 is intended to maximize the space for cell growth while ensuring the uniformity of the distribution of the biological ink, thereby improving the culture efficiency of the temperature control system of the organoid 3D printer. The first temperature control component 60 is installed close to the lower surface wall of the well plate 90, and the temperature value of the well plate 90 is adjusted to a first preset range suitable for cell growth through an electric heating or cooling element. The first preset range can be 32 degrees Celsius to 42 degrees Celsius to simulate the human body environment, such as 33 degrees Celsius, 36 degrees Celsius, 37 degrees Celsius, 39 degrees Celsius, 42 degrees Celsius, preferably 37 degrees Celsius. This structural design ensures that cells grow in a suitable temperature environment, thereby improving the efficiency and quality of biological reactions.

[0029] The present invention integrates the holding tanks 910 on the orifice plate 90, and the holding tanks 910 are connected by the orifice plate 90. The heat received by the orifice plate 90 can be conducted between the holding tanks 910, or the temperature of the solution in each holding tank 910 can be mutually conducted and mutually influenced through the orifice plate 90. Therefore, the design of the present application can make the temperature of each holding tank 910 tend to be average. Such a design is conducive to ensuring that the solution in each holding tank 910 has the same environmental conditions (such as temperature), and can more accurately and efficiently perform large-scale organoid printing, culture, reaction testing and other operations.

[0030] It is understood that in some embodiments, the first liquid guide assembly 40 can be equipped with a flow control valve to accurately control the flow of the bio-ink, so as to better meet different experimental requirements. In addition, the first drive assembly 70 can use a stepper motor or a servo motor, which has high-precision position control capabilities and can achieve fine-tuning of the bio-ink drop position, ensuring that the bio-ink can be accurately dripped into each receiving slot 910 of the orifice plate 90.

[0031] In some embodiments, the material of the orifice plate 90 can be selected to have excellent biocompatibility and thermal conductivity, such as glass, polystyrene, etc., which not only helps the adsorption and growth of cells, but also effectively transfers the heat generated by the first temperature control component 60 to maintain the uniformity of the temperature of the orifice plate 90.

[0032] In some embodiments, in addition to electric heating, the first temperature control component 60 can also be combined with a water bath circulation system to remove or supplement heat through water flow. This method is particularly suitable for experimental scenarios that require rapid temperature changes. For example, the first temperature control component 60 itself is a cavity with the function of accommodating coolant, and the first temperature control component 60 is concave to accommodate the orifice plate 90. The surrounding wall of the first temperature control component 60 fits the surrounding wall of the orifice plate 90 to increase the contact area between the first temperature control component 60 and the orifice plate 90, thereby improving the heat exchange efficiency. The first temperature control component 60 can also be a heat exchange pipe, which is wrapped around the outer periphery of the orifice plate 90, so as to achieve heat exchange between the coolant in the first temperature control component 60 and the orifice plate 90. In addition, in order to further improve the accuracy of temperature control, multiple temperature sensors can be arranged inside or on the surface of the orifice plate 90 to monitor the temperature changes of various parts in real time so as to adjust the temperature control strategy in time.

[0033] It is understood that in some embodiments, the number and arrangement of the receiving slots 910 on the well plate 90 can be adjusted according to the experimental requirements. For example, the well plate 90 can be designed to have 96 holes, 24 holes or 6 holes to accommodate experiments of different scales. The shape and size of the receiving slots 910 can also be optimized according to the cell type and culture conditions, such as round, square or oval.

[0034] The orifice plate 90 may be equipped with a cover to prevent external contaminants from entering the holding tank 910 and maintain a sterile environment. The cover may be designed to be a breathable material to allow gas exchange but prevent microorganisms from entering. In addition, the orifice plate 90 may also be equipped with a marking system, such as a number or letter mark, to facilitate the experimenter to quickly locate and record the position and content of each holding tank 910.

[0035] The manufacturing process of the orifice plate 90 can be injection molding or blow molding to ensure the dimensional accuracy and surface quality of the orifice plate 90. The edge of the orifice plate 90 can be designed to be wavy or serrated to increase the gripping force and facilitate the operation of the experimenter. The orifice plate 90 can also be equipped with a bracket to maintain its stability on the laboratory table.

[0036] Reference Figure 3 , Fig.10 and Fig.11 In some embodiments, the temperature control system of the organoid 3D printer includes a placement platform 302, and the orifice plate 90 and the first temperature control component 60 are respectively installed on opposite sides of the placement platform 302. The placement platform 302 is concave to form a first recessed portion, and the first recessed portion can accommodate the orifice plate 90. Since the concave structure shortens the distance between the orifice plate 90 and the first temperature control component 60, such a design ensures that the orifice plate 90 is stably placed, and also helps to maintain the close contact between the orifice plate 90 and the first temperature control component 60, thereby improving the temperature control speed of the orifice plate 90 by the first temperature control component 60. The side of the placement platform 302 away from the first recessed portion is recessed in the opposite direction of the concave direction of the first recessed portion to form a second recessed portion, and the second recessed portion is used to install the first temperature control component 60. Such a design not only saves space, but also shortens the distance between the first temperature control component 60 and the orifice plate 90, and can ensure that the first temperature control component 60 can effectively and quickly heat or cool the orifice plate 90, thereby ensuring the efficiency and stability of temperature control.

[0037] It is understood that in some embodiments, the placement platform 302 can be designed as a modular structure, allowing the user to replace the orifice plate 90 or the temperature control component of different specifications according to the experimental requirements, thereby improving the flexibility and applicability of the system. For example, the orifice plate 90 and the temperature control component can be quickly installed and removed by means of a slide rail or a buckle, which is convenient for maintenance and cleaning.

[0038] The material of the placement platform 302 can be selected to have good corrosion resistance and thermal stability to cope with the chemical substances and temperature changes that may be generated during the biological reaction. In addition, in some embodiments, a layer of heat insulation material can be added to the bottom of the placement platform 302 to prevent heat from diffusing to the outside and improve energy utilization efficiency. In some cases, the placement platform 302 can also be equipped with a temperature monitoring system to monitor the temperature of the orifice plate 90 and the temperature control component in real time through an integrated temperature sensor to ensure that the entire system is in the best working condition.

[0039] Reference Figure 3 , Fig.10 and Fig.11 In some embodiments, the first temperature control assembly 60 includes a first temperature control device 304 and a second temperature control device 305. The first temperature control device 304 is fixed to the placement platform 302 and fits the lower surface wall of the orifice plate 90 to adjust the overall temperature of the orifice plate 90. The second temperature control device 305 is movably connected to the placement platform 302 and can adjust the local temperature of the orifice plate 90. This design allows the orifice plate 90 to be finely adjusted while the overall temperature is controlled, thereby better meeting the needs of different cell growth. It can be understood that the movable connection of the second temperature control device 305 enables the second temperature control device 305 to be configured to compensate for the temperature imbalance area in time. For example, during the reaction, if the temperature of a certain holding tank 910 deviates from the preset value, the second temperature control device 305 can be driven to move to the area to adjust the temperature of the solution in the holding tank 910 and correct the reaction process in time. Of course, in some embodiments, only part of the orifice plate 90 is injected with biological ink or the solution to be treated. At this time, it is only necessary to drive the second temperature control device 305 to move to the area where the containing tank 910 containing the biological ink or the solution to be treated is located, and only the temperature of this area is adjusted, thereby saving power and reducing energy consumption.

[0040] It is understood that, in some embodiments, the first temperature control device 304 may be an electric heating plate or a cooling plate, which has a high thermal response speed and temperature control accuracy, and can quickly adjust the temperature of the orifice plate 90 to a desired range. The second temperature control device 305 may be a small heating rod or a cooling rod, and by precisely controlling its position and power, the temperature of a local area of ​​the orifice plate 90 can be adjusted. For example, the second temperature control device 305 can be designed as a movable module, which moves on the surface of the orifice plate 90 by means of a slide rail or a mechanical arm to cover different local areas.

[0041] In some embodiments, the second temperature control device 305 can be equipped with a temperature sensor to monitor the temperature change of the local area in real time to ensure the accuracy and stability of temperature control. In order to improve the reliability and durability of the system, the connection between the first temperature control device 304 and the second temperature control device 305 can be waterproof and dustproof to prevent liquid or dust from entering and affecting the temperature control effect.

[0042] Reference Figure 3 , Fig.10 and Fig.11In some embodiments, the first temperature control assembly 60 includes a drive device, which is connected to the second temperature control device 305. The drive device is configured to drive the second temperature control device 305 to move relative to the orifice plate 90 to control the temperature of the area of ​​the orifice plate 90 containing the biological ink. This design enables the second temperature control device 305 to move flexibly on the surface of the orifice plate 90 to achieve local temperature control of different areas, thereby better adapting to the needs of different cell growth. Moreover, such a design can compensate for areas with too low temperatures or areas with too high temperatures. It can be understood that when only the temperature of a local area of ​​the orifice plate 90 needs to be adjusted, it is only necessary to control the second temperature control device 305 to regulate the temperature of the corresponding area, which can save power and make the organoid 3D printer temperature control system of the present application more environmentally friendly.

[0043] It is understood that, in some embodiments, the drive device may adopt a stepper motor or a servo motor, which has high-precision position control capability and a large driving force, and can accurately move the second temperature control device 305 to the desired position. The drive device can be equipped with a closed-loop control system, which adjusts the position and speed of the motor in real time through feedback signals to ensure the movement accuracy and stability of the second temperature control device 305. In addition, the drive device can be designed as a modular structure for easy maintenance and replacement. For example, the drive device may include a detachable motor module and a slide rail module, the slide rail module is mounted on the placement platform 302, and the motor module drives the second temperature control device 305 to move along the slide rail through a belt or a lead screw. In order to improve the reliability and safety of the system, the drive device can be equipped with overload protection and emergency stop functions to prevent damage or accidents caused by unexpected situations.

[0044] In order to cooperate with the work of the driving device, in some embodiments, the placement platform 302 can be added with a guide groove or a positioning pin to ensure that the second temperature control device 305 remains stable during the movement and avoids temperature control deviation caused by vibration or collision. In addition, wireless communication technology can be used between the driving device and the second temperature control device 305 to achieve remote control and monitoring, thereby improving the intelligence level of the system.

[0045] Reference Figure 3 , Fig.10 and Fig.11 In some embodiments, the placement platform 302 includes a plurality of upwardly protruding stoppers, which abut against the edge of the orifice plate 90. The design of the stoppers ensures the stability and precise position of the orifice plate 90 on the placement platform 302, and prevents the orifice plate 90 from being displaced or tilted during operation. This structural design not only improves the installation accuracy of the orifice plate 90, but also enhances the overall stability of the system.

[0046] It is understood that in some embodiments, the stopper can be made of elastic material, such as silicone or rubber, so that it can better adapt to the size change of the orifice plate 90, while providing a buffering effect and reducing the wear of the orifice plate 90 during installation and use. In addition, the stopper can be designed as an adjustable structure, fixed on the placement platform 302 by means of screws or buckles, and the user can adjust the position of the stopper according to the orifice plate 90 of different specifications to ensure the accurate installation of the orifice plate 90. The height and shape of the stopper can also be optimized according to the edge design of the orifice plate 90. For example, the stopper can be designed to be L-shaped or U-shaped to provide better support and fixing effect. In order to further improve the reliability of the system, an anti-skid pad can be added to the surface where the stopper contacts the orifice plate 90 to prevent the orifice plate 90 from sliding during operation. In addition, the material selection of the stopper should consider biocompatibility and chemical corrosion resistance to adapt to various chemical substances that may be produced during the biological reaction process.

[0047] Reference Figure 3 , Fig.10 and Fig.11 In some embodiments, the temperature control system of the organoid 3D printer further includes a temperature probe 303, which is connected to the placement platform 302 and is located on the side of the placement platform 302 away from the orifice plate 90. The temperature probe 303 is designed to monitor the temperature of the orifice plate 90 in real time to ensure that the temperature of the orifice plate 90 is always maintained within a first preset range. The temperature probe 303 is connected to the temperature control system controller and can provide real-time feedback of temperature data. The controller adjusts the working state of the first temperature control component 60 according to the feedback data, thereby achieving precise temperature control. This design ensures the stability and reliability of the temperature of the orifice plate 90 and provides an ideal environment for cell growth.

[0048] It is understood that in some embodiments, the temperature probe 303 can be a high-precision thermocouple or thermal resistor. These sensors have high temperature measurement accuracy and response speed, and can accurately monitor the temperature changes of the orifice plate 90 in real time. The temperature probe 303 can be designed as a detachable structure to facilitate regular calibration and maintenance to ensure its accuracy and reliability in long-term use. In addition, multiple temperature probes 303 can be equipped and distributed at different positions of the orifice plate 90 to monitor the overall temperature distribution of the orifice plate 90 to ensure the uniformity of temperature. The communication between the temperature probe 303 and the controller can be wired or wireless. The wireless communication method can reduce the complexity of wiring and improve the flexibility of the system.

[0049] In some embodiments, in order to prevent the temperature probe 303 from being affected by the external environment, a protective cover can be added around the temperature probe 303 to protect the probe from interference by pollutants such as dust and liquid. In addition, the installation position of the temperature probe 303 should be as close as possible to the lower surface wall of the orifice plate 90 to ensure the accuracy and real-time performance of the temperature measurement. The material selection of the temperature probe 303 should take into account biocompatibility and high temperature resistance to adapt to the possible high temperature environment during the biological reaction process.

[0050] Reference Figures 1 to 7 In some embodiments, the organoid 3D printer temperature control system further includes a housing assembly 10, the housing assembly 10 having an inner chamber, the inner chamber being used to accommodate a first liquid guide assembly 40, a first drive assembly 70, an orifice plate 90, and a first temperature control assembly 60. The design of the housing assembly 10 ensures the closedness and sterile environment of the entire system. The housing assembly 10 includes a filter fan 101, the filter fan 101 includes a filter screen and a guide air duct, the filter screen and the guide air duct are suitable for connecting the inner chamber and the external environment to ensure that the inner chamber provides a sterile environment. This design not only protects the internal components from contamination, but also provides ideal environmental conditions for cell growth.

[0051] It is understandable that in some embodiments, the material of the housing assembly 10 can be selected from materials with excellent biocompatibility and chemical corrosion resistance, such as stainless steel or polypropylene, to ensure stability and reliability in long-term use. The design of the inner chamber can include multiple vents and sealed doors, the vents are used to install the filter fan 101, and the sealed doors are used to facilitate users to replace or maintain internal components under sterile conditions. The filter screen of the filter fan 101 can use HEPA high-efficiency filter material to ensure that particulate matter and microorganisms in the air are effectively filtered. The design of the guide air duct should ensure uniform air flow to avoid eddy currents or dead corners, thereby improving the quality of the sterile environment.

[0052] In some embodiments, the housing assembly 10 can be equipped with a pressure sensor and a temperature and humidity sensor to monitor the environmental parameters of the inner chamber in real time to ensure that it is always in the best condition. In order to improve the automation of the system, the housing assembly 10 can also integrate an automatic disinfection function to regularly disinfect the inner chamber with ultraviolet light or chemical disinfectants to further ensure a sterile environment. The bottom of the housing assembly 10 can be designed as a drainable structure to facilitate cleaning and discharge of wastewater generated during the cleaning process.

[0053] Reference Figure 8 and Fig. 9In some embodiments, the temperature control system of the organoid 3D printer includes a second temperature control component 50, which is connected to the liquid guide component and is used to adjust the temperature of the biological ink exported by the first liquid guide component 40 to a second preset range. This design ensures that the biological ink is at a suitable temperature before being exported, thereby improving the survival rate of cells and the efficiency of biological reactions. The second temperature control component 50 can be an independent temperature control unit, which is connected to the first liquid guide component 40 through a pipeline to ensure the temperature stability of the biological ink during the transmission process.

[0054] It is understood that in some embodiments, the second temperature control component 50 can use a water bath circulation system or an electric heating / cooling plate, which have high temperature control accuracy and response speed and can quickly adjust the temperature of the bio-ink to a desired range. The water bath circulation system removes or replenishes heat through circulating water, which is suitable for scenes that require long-term stable temperature, while the electric heating / cooling plate is suitable for scenes that require rapid temperature changes.

[0055] In some embodiments, the second temperature control component 50 can be equipped with a temperature sensor to monitor the temperature changes of the biological ink in real time to ensure the accuracy and stability of temperature control. In order to improve the reliability and safety of the system, the second temperature control component 50 can be designed as a modular structure for easy maintenance and replacement. For example, the temperature control unit can be designed as a detachable module, connected to the liquid guide component through a quick connector, which is convenient for users to perform regular inspections and maintenance. In addition, the second temperature control component 50 can be equipped with a flow control valve to further optimize the temperature control effect by adjusting the flow rate. In order to prevent the biological ink from being contaminated during the transmission process, the connecting pipe between the liquid guide component and the second temperature control component 50 can adopt a disposable sterile pipeline to ensure that it is sterile each time it is used. The second temperature control component 50 can also be equipped with data recording and transmission functions, and the temperature data is transmitted to the central control system in real time through wireless communication technology, which is convenient for users to remotely monitor and manage.

[0056] Reference Figure 8 and Fig. 9In some embodiments, the first driving assembly 70 includes a motor, a transmission mechanism and a guide rail. The motor is installed in the inner chamber of the housing assembly 10, the transmission mechanism connects the motor and the first liquid guide assembly 40, and the guide rail is used to guide the movement of the first liquid guide assembly 40. When the motor is started, the first liquid guide assembly 40 is driven by the transmission mechanism to move along the guide rail according to a predetermined path. First, the first liquid guide assembly 40 is driven to move in the direction of the solution to be treated, so that the first liquid guide end contacts the solution to be treated, and the biological ink will adhere to the surface of the solution to be treated due to capillary action. Subsequently, the motor reverses and pulls the first liquid guide assembly 40 back through the transmission mechanism, so that the biological ink falls off from the first liquid guide end, completing the fixed-point and quantitative delivery of the biological ink. This structural design ensures that the biological ink can be accurately placed at the target position, while avoiding the residue of the biological ink, so that the volume of the biological ink in the receiving tank 910 can be further accurately controlled, and the accuracy of the temperature control system of the organoid 3D printer can be improved.

[0057] The organoid 3D printer temperature control system of the present application can also be used in various application scenarios such as drug screening, screening of different growth and development factors, exploration of cell mechanisms, and manufacturing of multiple tissues.

[0058] In some embodiments, the first liquid guide component 40 includes a linear pump and a first liquid guide end. The linear pump is used to store and squeeze the bio-ink, and the first liquid guide end is connected to the linear pump. When the linear pump is working, the bio-ink is pushed out from the first liquid guide end through the reciprocating motion of the internal piston. The design of the first liquid guide component 40 allows for precise control of the discharge amount of the bio-ink, ensuring that the volume of the bio-ink discharged each time is consistent.

[0059] The first driving component 70 is connected to the first liquid guiding component 40, and is composed of a motor and a screw. The motor drives the screw to rotate, and the screw drives the first liquid guiding component 40 to move along the first preset path. The first driving component 70 drives the first liquid guiding component 40 to move, so that the biological ink droplets suspended at the first liquid guiding end will contact and adhere to the previously derived biological ink liquid surface, and then the first driving component 70 drives the first liquid guiding component 40 away from the previously derived biological ink liquid surface, so that the biological ink droplets previously suspended at the first liquid guiding end can be smoothly extracted from the first liquid guiding end. This design avoids droplet residue and can accurately control the volume of biological ink discharged by the first liquid guiding component 40.

[0060] Of course, it can be understood that the solution discharged by the first liquid guiding component 40 can be biological ink, and the biological ink can include cell suspension, cell mixed hydrogel, cell sphere, cell tissue block, cell fiber, or cell cluster, hydrogel, DNA, etc. The corresponding material is selected according to the actual application. Through the culture device of the present application, the volume of these materials discharged can be more accurately controlled, that is, the amount of materials participating in the reaction can be more accurately controlled.

[0061] It is understandable that in some embodiments, the linear pump of the first liquid guiding component 40 can be driven in different ways, such as a pneumatic pump or an electric pump, as long as the accurate discharge of the biological ink can be achieved. For example, the parameters of the linear pump of the first liquid guiding component 40 can be configured as 100ul, 200ul, 500ul, 1ml, 2ml, 5ml, 10ml, 50ml, 100ml, etc., and the first liquid guiding end can be a needle, and the diameter of the needle can be 0.1mm, 0.2mm, 0.3mm, 0.5mm.

[0062] It is understandable that the volume of the bio-ink droplets discharged from the first liquid-conducting end is determined by the cross-sectional area and lead of the outlet, so in some embodiments, the first liquid-conducting end can be designed into different shapes and sizes to accommodate different types of bio-inks and experimental requirements. For example, the first liquid-conducting end can be designed into an elongated shape to form small bio-ink droplets, or designed into a flat shape to form larger bio-ink droplets.

[0063] The motion control of the first liquid guiding component 40 by the first driving component 70 in order to avoid the residue of droplets at the first liquid guiding end can be a compensating type or a hovering type. For example, when configured as a compensating type, the first driving component 70 continuously drives the first liquid guiding component 40 to move away from the liquid surface of the biological ink, that is, the liquid guiding component moves up while dripping liquid, until the last moment, the dripping biological ink is pulled by the liquid surface of the biological ink and is torn off from the first liquid guiding end, so as to achieve the effect of accurately controlling the volume of the biological ink. When configured as a hovering type, the first driving component 70 only needs to drive the first liquid guiding end to a preset height. As the biological ink is injected, the liquid level of the biological ink rises. When approaching the preset height, the liquid surface of the biological ink will contact the biological ink droplet suspended from the first liquid guiding end, thereby tearing the drop of biological ink off from the first liquid guiding end. It can be understood that the volume of the bio-ink in the container is determined by the volume of the last drop of bio-ink discharged from the first liquid-conducting end. The finer the volume of the last drop of bio-ink discharged from the first liquid-conducting end, the finer the volume of the bio-ink in the container, that is, the higher the precision. Therefore, whether the compensation type or the suspension type is adopted, it is only necessary to ensure that the last drop of bio-ink discharged from the first liquid-conducting end is torn off from the first liquid-conducting end by being adsorbed by the previous bio-ink liquid surface, thereby detaching from the first liquid-conducting end.

[0064] In order to accurately control the amount of bio-ink in the receiving tank 910, it is necessary to accurately control the height of the first liquid guide end from the bio-ink liquid surface or the orifice plate 90. In some embodiments, the accuracy is accurately controlled by a grating ruler to ensure that the accuracy of the culture device can achieve the preset effect.

[0065] In some embodiments, the first temperature control component 60 includes a water-cooled generator, which can use a variety of cooling media, such as water, ethanol or other coolants, to meet different temperature regulation needs. The liquid cooling pipe can be designed to be serpentine or spiral to increase the contact area between the coolant and the bio-ink and improve the cooling efficiency. In addition, the first temperature control component 60 can also be equipped with a temperature sensor to monitor the temperature of the bio-ink in real time to ensure that the temperature is always within the first preset range. In some embodiments, the first preset range is 4 degrees Celsius to 8 degrees Celsius. For example, the temperature can be selected as 4 degrees Celsius, 5 degrees Celsius, 6 degrees Celsius, 7 degrees Celsius, and 8 degrees Celsius.

[0066] The motor of the first driving component 70 can be a stepper motor or a servo motor to achieve high-precision motion control. The screw rod can be designed with different pitches to adjust the moving speed and distance of the first liquid guiding component 40. The first driving component 70 can also be equipped with an encoder to provide real-time feedback on the position information of the first liquid guiding component 40 to ensure that it accurately reaches the predetermined position.

[0067] Reference Figures 6 to 9 In some embodiments, the culture device further includes a second liquid guiding component 20 and a second driving component 80. The second liquid guiding component 20 has a second liquid guiding end for guiding out the culture solution, and the second driving component 80 is connected to the second liquid guiding component 20. The second driving component 80 drives the second liquid guiding component 20 to move along the second preset path to inject the culture solution into the receiving groove 910 of the well plate 90.

[0068] The design of the second liquid guide component 20 is similar to the first liquid guide component 40, and includes a liquid storage container and a liquid guide pipe. The liquid storage container is used to store the culture fluid, and the liquid guide pipe connects the liquid storage container and the second liquid guide end, and the culture fluid is discharged from the second liquid guide end through the operation of the internal pump. The design of the second liquid guide end ensures the accurate discharge of the culture fluid and avoids the situation of excess or deficiency.

[0069] The second driving assembly 80 is composed of a motor and a screw rod. The motor drives the screw rod to rotate, and the screw rod drives the second liquid guiding assembly 20 to move along the second preset path. When the second liquid guiding assembly 20 reaches the predetermined position, the culture liquid is guided out from the second liquid guiding end and injected into the receiving groove 910 of the orifice plate 90. This design ensures the accurate distribution of the culture liquid and improves the efficiency and accuracy of the experiment.

[0070] It is understood that in some embodiments, the liquid storage container of the second liquid guide assembly 20 can be made of different materials, such as stainless steel or plastic, to accommodate different types of culture fluids. The capacity of the liquid storage container can be adjusted according to experimental requirements to meet experiments of different scales. The liquid guide pipe can be designed to have different diameters and lengths to accommodate different flow rates and distances.

[0071] The motor of the second driving component 80 can be a stepper motor or a servo motor to achieve high-precision motion control. The screw rod can be designed with different pitches to adjust the moving speed and distance of the second liquid guiding component 20. The second driving component 80 can also be equipped with an encoder to provide real-time feedback on the position information of the second liquid guiding component 20 to ensure that it accurately reaches the predetermined position.

[0072] In some embodiments, the second liquid guide component 20 may further include a diverter plate having a plurality of diverter ports, each of which corresponds to a receiving tank 910. The design of the diverter plate allows the culture fluid to be injected into a plurality of receiving tanks 910 at the same time, thereby improving the efficiency of the experiment. The shape and size of the diverter ports may be optimized according to the size of the receiving tank 910 to ensure uniform distribution of the culture fluid.

[0073] In some embodiments, a heating or cooling function may be integrated on the second liquid guide assembly 20 to adjust the temperature of the culture solution to ensure that it reaches the required temperature condition before being injected into the well plate 90. These improvements further improve the reliability of the system and the accuracy of the experimental results.

[0074] In some embodiments, regarding the temperature control system of the organoid 3D printer of the present application, refer to Figures 1 to 11 , specifically, refer to Figures 1 to 3 The temperature control system of the organoid 3D printer is mainly composed of a shell component 10, a second liquid guide component 20, a culture platform unit 30, a first liquid guide component 40, a first temperature control component 60, and a second temperature control component 50.

[0075] The housing assembly 10 in the extrusion-type organoid 3D printer of the present invention is shown in FIG. Figure 4 and Figure 5 The housing assembly 10 includes a filter fan 101, a high-efficiency filter 102, a microscope display screen 103, an outer door panel 104, a cabinet rear end support 105, a machine support unit 106, a load-bearing foot cup 107, and a cabinet lower end support 108.

[0076] The filter fan 101 in the extrusion-type organoid 3D printer of the present invention has a special FFU structure, a guide air duct and an air distribution system design, which can reduce eddy currents, eliminate noise, guide airflow to pass through the filter screen evenly, and can control the wind speed. With the high-efficiency filter screen, a sterile environment of the entire internal environment can be achieved in a short time. The microscope display screen 103 is connected to the microscope through a cable, and the captured video images can be transmitted to the display screen in real time. The outer door panel 104 has two left and right panels, which are made of translucent black acrylic material, and can achieve a semi-transparent effect of the internal structure of the machine. The rear end support 105 plays a supporting role, and there are regular heat dissipation holes in the middle for heat dissipation of the machine. The support units 106 are all hollowed out in the middle to reduce the weight of the machine, and serve as support parts for the components on the machine to ensure the smooth operation of the machine. The load-bearing foot cups 107 are distributed at the four corners of the machine, and the height of the machine operating platform can be adjusted up and down by a small margin. The lower end support 108 of the cabinet is connected to the rear end support 105 of the cabinet, and regular heat dissipation holes are symmetrically distributed in the middle of the cabinet for heat dissipation of the machine.

[0077] The second liquid guide assembly 20 in the bioreactor of the present invention is Fig. 9 The second liquid guide component includes a vertical precision screw motor 201, a motor slider 202, a hydraulic block 203, a liquid injection pump 204, a liquid packaging shell 205, a liquid adding device fixture 206, a microscope 207, and a microscope slide 208. The vertical precision screw motor 201 is precisely driven and is responsible for providing power for the liquid adding component to move up and down. Including but not limited to transmission in the form of belts, chains, gears, etc. The motor slider 202 is closely matched with the vertical precision screw motor 201, and the screw motor rotates forward and backward to realize the up and down sliding of the slider. One end of the hydraulic block 203 is connected to the liquid injection pump, and the other end is connected to the liquid adding power unit. When it is tightly fitted with the liquid injection pump 204, the downward movement of the hydraulic block 203 will cause the liquid adding material to be squeezed out from the injection needle. The liquid injection pump 204 is composed of a liquid adding push rod, a liquid adding pump shell and an injection needle, and has good airtightness. The size of the liquid injection pump 204 can be adjusted according to the amount of liquid added. The liquid adding package shell 205 serves as a fixing unit for the liquid adding injection pump 204 and plays a supporting role. The liquid adding device fixture 206 serves as a fixing plate for the second liquid guiding assembly 20, and is equipped with another set of power units for pressing the liquid adding block a203, which is placed on the back of the fixing plate.

[0078] The microscope 207 in the extrusion-type organoid 3D printer of the present invention can observe the situation of spotting and adding liquid in real time, and provide real-time feedback to the system control unit, so that the spotting can be supplemented for the individual wells that have not been spotted. The microscope slide 208 can slide up and down and then be fixed to adjust the imaging format of the microscope.

[0079] The first liquid guide component 40 in the bioreactor of the present invention is Fig. 9The first liquid guide component includes a vertical precision screw motor 401, a motor slider 402, a sample spotting pressure block 403, a sample spotting injection pump 404, a sample spotting packaging shell 405, a sample spotting circulating water cooling generator 406, a temperature probe 407, and a sample spotting device holder 408. The vertical precision screw motor 401 is a precision transmission, responsible for providing power for the sample spotting component to move up and down. Including but not limited to transmission in the form of belts, chains, gears, etc. The motor slider 402 is closely matched with the vertical precision screw motor 401, and the screw motor rotates forward and backward to realize the up and down sliding of the slider. The sample spotting pressure block 403 is connected to the sample spotting injection pump at one end and the sample spotting power unit at the other end. When it is tightly fitted with the sample spotting injection pump, the downward movement of the sample spotting pressure block 403 will cause the sample spotting material to be squeezed out from the injection needle. The sample spotting injection pump 404 is composed of a sample spotting push rod, a sample spotting pump shell and an injection needle, and has good airtightness. The size of the sample spotting injection pump can be adjusted according to the sample spotting amount. The spotting packaging shell 405 serves as a fixing unit of the spotting injection pump 404, plays a supporting role, and can transfer temperature. The other end of the spotting circulating water-cooled generator 406 is connected to the water pump, and the excess temperature is taken away through water circulation. The temperature probe 407 can detect the temperature of the spotting area in real time. When the predetermined temperature range is reached, the temperature control module will stop working. On the contrary, if it is not in the predetermined temperature range, the temperature control module will start the working mode, and the normal temperature is set at about 4 degrees Celsius to 8 degrees Celsius. The spotting device fixture 408 serves as a fixing plate of the first liquid guide component 40, on which is mounted another set of power units of the spotting pressure block 403, which are placed on the back of the fixing plate.

[0080] The culture platform unit 30 in the bioreactor of the present invention is Fig.10 and Fig.11 The culture platform unit includes a well plate holder 301, a placement platform 302, a temperature probe 303, a circulating water cooler, and a semiconductor temperature controller. The first temperature control device 304 can be configured as a circulating water cooler, and the second temperature control device 305 can be configured as a semiconductor temperature controller, and of course the two can also be reversed. The well plate holder 301 can place well plates of different sizes and numbers of holes therein through the surrounding fixed holes to fix the position of each empty space. The placement platform 302, the well plate holder 301 can be placed on the top, and the first temperature control component 60 is connected to the bottom. The material has good thermal conductivity, and the temperature regulated by the first temperature control component 60 can be transmitted to the well plate 90 in time. The temperature probe 303 can detect the temperature of the placement platform 302 in real time. When the predetermined temperature range is reached, the first temperature control component 60 will stop working. On the contrary, if it is not in the predetermined temperature range, the temperature control module will start the working mode, and the normal temperature is set at around 37 degrees Celsius. The other end of the circulating water cooler is connected to a water pump, which takes away the excess temperature through water circulation. The semiconductor temperature controller has one end for cooling and the other end for generating heat. The heat generating surface can be attached to the mounting platform 302 to transfer the temperature to the orifice plate 90 .

[0081] The above are only preferred embodiments of the present invention, and are not intended to limit the patent scope of the present invention. All equivalent structural changes made using the contents of the present invention's specification and drawings, or directly / indirectly applied in other related technical fields, are included in the patent protection scope of the present invention.

Claims

1. A temperature control system for an organoid 3D printer, characterized in that: The culture device comprises: A first liquid guiding component, comprising a first liquid guiding end for guiding out the biological ink; a first driving component, connected to the first liquid guiding component, and used to drive the first liquid guiding component to move; An orifice plate is disposed below the first liquid guiding component, the orifice plate comprises an upper surface wall and a lower surface wall, the upper surface wall is concavely provided with a plurality of receiving grooves, each of the receiving grooves is used to receive the biological ink guided out by the first liquid guiding end; The first temperature control component is attached to the lower surface wall, and the first temperature control component is used to adjust the temperature value of the orifice plate to a first preset range.

2. The organoid 3D printer temperature control system according to claim 1, characterized in that: The organoid 3D printer temperature control system comprises a placement platform, and the orifice plate and the first temperature control component are respectively arranged on opposite sides of the placement platform; Among them, the placement platform is recessed to form a first recessed portion, and the first recessed portion is used to accommodate the orifice plate. The side of the placement platform away from the first recessed portion is recessed in the opposite direction of the recessed direction of the first recessed portion to form a second recessed portion, and the second recessed portion is used to accommodate the first temperature control component.

3. The organoid 3D printer temperature control system according to claim 2, characterized in that: The first temperature control assembly includes a first temperature control device and a second temperature control device, the first temperature control device is fixed to the placement platform, and the first temperature control device is attached to the lower surface wall of the orifice plate to adjust the temperature of the orifice plate; The second temperature control device is movably connected to the placement platform to adjust the local temperature of the orifice plate.

4. The organoid 3D printer temperature control system according to claim 3, characterized in that: The first temperature control component includes a driving device, which is connected to the second temperature control device. The driving device is configured to drive the second temperature control device to move relative to the orifice plate to adjust the temperature of the area of ​​the orifice plate containing the biological ink.

5. The organoid 3D printer temperature control system according to claim 2, characterized in that: The placement platform includes a plurality of upwardly protruding stoppers, and the plurality of stoppers all abut against the edge of the orifice plate.

6. The organoid 3D printer temperature control system according to claim 2, characterized in that: The organoid 3D printer temperature control system also includes a temperature probe, which is connected to the placement platform and is located on a side of the placement platform away from the orifice plate.

7. The organoid 3D printer temperature control system according to claim 1, characterized in that: The organoid 3D printer temperature control system includes a second temperature control component, which is connected to the first liquid conducting component, and the second temperature control component is used to adjust the temperature of the biological ink exported by the first liquid conducting component to a second preset range.

8. The organoid 3D printer temperature control system according to claim 1, characterized in that: The temperature control system of the organoid 3D printer includes a first driving component, the first driving component is connected to the first liquid guiding component, and the first driving component is used to drive the first liquid guiding component to move along a first preset path; Among them, the first driving component is configured to first move the liquid guiding component in a direction close to the solution to be treated until the biological ink discharged from the first liquid guiding end adheres to the solution to be treated, and then move the first liquid guiding component in a direction away from the solution to be treated until the biological ink separates from the first liquid guiding end.

9. The organoid 3D printer temperature control system according to claim 1, characterized in that: The temperature control system of the organoid 3D printer also includes a second liquid conducting component and a second driving component, the second liquid conducting component has a second liquid conducting end for conducting culture fluid, the second driving component is connected to the second liquid conducting component, and the second driving component drives the second liquid conducting component to move along a second preset path to inject the culture fluid into the holding tank.

10. The organoid 3D printer temperature control system according to claim 1, characterized in that: The organoid 3D printer temperature control system also includes a shell component, which has an inner chamber, and the inner chamber can seal and accommodate the first liquid guide component, the first drive component, the orifice plate and the first temperature control component.

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