Automatic embryo culture and monitoring management system

Abstract

The invention belongs to the technical field of biomedicine, and provides an automatic embryo culture and monitoring management system which comprises a gas supply module, an embryo culture module, an optical monitoring module and a liquid path assembly, and the gas supply module, the embryo culture module and the optical monitoring module are electrically connected in sequence; according to the invention, a plurality of gases required by embryo culture are provided through the gas supply module, and online precise adjustment of gas concentration is realized, so that the stability and controllability of an embryo culture environment are ensured, and the culture success rate is improved; meanwhile, the optical monitoring module monitors the embryo growth state in real time, an intelligent evaluation algorithm is introduced, and the monitoring precision and the intelligent level are improved; the liquid path assembly realizes on-line automatic replacement of the culture solution, and the replacement opportunity is optimized in combination with a prediction algorithm, so that the embryo growth is prevented from being influenced by the culture solution problem.

Description

Technical Field

[0001] The invention belongs to the field of biomedical technology, and in particular is an automated embryo culture and monitoring management system. Background Art

[0002] In biomedicine, embryo culture is a crucial link, and its process involves the precise control and monitoring of embryos. Traditional embryo culture methods mostly rely on manual operations, including gas supply and regulation, culture medium replacement, and embryo growth status monitoring. However, this manual operation method has many shortcomings, such as cumbersome operation, high error rate, and difficulty in achieving precise control of the embryo culture environment. In addition, due to the limitations of manual monitoring, abnormal conditions in the embryo growth process are often not discovered in time, thus affecting the embryo's development quality and success rate.

[0003] In recent years, with the rapid development of automation technology, more and more fields have begun to try to apply automation technology to the embryo culture process; however, most of the existing automated embryo culture systems can only realize the automation of some functions, such as automatic gas supply or automatic replacement of culture medium, but cannot achieve comprehensive automated monitoring and management of the entire embryo culture process.

[0004] To this end, those skilled in the art have proposed an automated embryo culture and monitoring management system to solve the problems raised in the background art. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides an automated embryo culture and monitoring management system to solve the problem that most automated embryo culture systems in the prior art can only automate part of the functions and thus cannot achieve comprehensive automated monitoring and management of the entire embryo culture process.

[0006] An automated embryo culture and monitoring management system, comprising:

[0007] Gas supply module, used to provide a variety of gases required for embryo culture, and capable of online adjustment of gas concentration;

[0008] An embryo culture module, used for carrying embryos and providing a culture environment, comprises an embryo culture chamber and a plurality of gas distribution branches corresponding to the embryo culture chambers, wherein the gas distribution branches are used for delivering gas to the embryo culture chambers;

[0009] An optical monitoring module, used for monitoring the embryos in the embryo culture chamber;

[0010] The liquid circuit assembly is used to realize online automatic replacement of culture fluid and comprises a plurality of liquid distribution branches corresponding to the embryo culture chambers one by one. The liquid distribution branches are used to transport and discharge culture fluid to the embryo culture chambers.

[0011] Preferably, the gas supply module comprises a multi-channel gas source supply unit and a gas mixing device, wherein the multi-channel gas source supply unit is used to provide a variety of gases, and the gas mixing device is used to mix the multiple gases in a specific ratio and then transport them to the embryo culture chamber.

[0012] Preferably, in the gas supply module, in order to achieve more precise online regulation of gas concentration, an intelligent control algorithm is introduced. The algorithm dynamically adjusts the supply ratio and flow rate of each gas according to the gas concentration data monitored in real time in the embryo culture room to ensure that the gas concentration in the culture environment is always in the optimal state.

[0013] Preferably, the embryo culture module further comprises an embryo cover, which is used to confine the embryo in the space inside the embryo cover, and the outer wall of the embryo cover is provided with a plurality of through holes of a size that allows the culture solution to pass through but prevents the embryo from passing through.

[0014] Preferably, the optical monitoring module observes the embryos cultured in the embryo cover through an observation channel, and the observation channel is formed by the first glass window, the embryo cover and the second glass window being positioned opposite to each other in the vertical direction.

[0015] Preferably, in the optical monitoring module, in order to improve the monitoring accuracy and intelligence level of the embryo growth status, an embryo growth status evaluation algorithm is introduced to automatically identify and extract the key growth characteristics of the embryo, including morphology, size, color, etc., so as to perform an intelligent evaluation of the embryo's growth status.

[0016] Preferably, the liquid circuit assembly further comprises a waste liquid container connected to the ends of the liquid distribution branch pipes for collecting the discharged culture liquid.

[0017] Preferably, in the liquid circuit component, in order to optimize the timing of replacing the culture medium, a prediction algorithm is introduced. This algorithm can predict when the culture medium needs to be replaced based on factors such as the growth rate of the embryo and the consumption of nutrients in the culture medium, so as to avoid affecting the growth of the embryo due to aging of the culture medium or insufficient nutrients.

[0018] Preferably, the system further comprises a culture medium balance chamber for storing the culture medium and delivering the culture medium to the embryo culture chamber through the fluid path assembly.

[0019] Preferably, the system introduces a multi-objective optimization algorithm to find the optimal combination of system parameters.

[0020] A processor is configured to execute the above-mentioned automated embryo culture and monitoring management system.

[0021] A computer-readable storage medium stores a computer program, which, when executed by a processor, implements the above-mentioned automated embryo culture and monitoring management system.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. The present invention provides a variety of gases required for embryo culture through a gas supply module, and can achieve online adjustment of gas concentration, thereby ensuring the stability and controllability of the embryo culture environment; this not only improves the success rate of embryo culture, but also reduces the risk of abnormal embryo development due to environmental factors.

[0024] 2. The present invention uses an optical monitoring module to monitor the embryos in the embryo culture room in real time, and introduces an embryo growth status evaluation algorithm to automatically identify and extract key growth characteristics of the embryo, such as morphology, size, color, etc., to achieve intelligent evaluation of the embryo growth status. This not only improves the monitoring accuracy of the embryo growth status, but also provides scientific researchers with more intuitive and accurate embryo growth data, which is helpful for the development of scientific research and the optimization of embryo culture technology.

[0025] 3. The present invention realizes online automatic replacement of culture medium through a liquid circuit assembly, and introduces a prediction algorithm to optimize the replacement timing of the culture medium, thereby avoiding the problem of embryo growth being affected by aging of the culture medium or insufficient nutrients, thereby improving the growth rate and development quality of the embryo.

[0026] 4. The present invention also introduces a multi-objective optimization algorithm to find the optimal combination of system parameters, which not only improves the overall performance of the system, but also enables the system to flexibly adjust parameters when facing different types of embryo culture requirements to achieve personalized embryo culture plans. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a framework diagram of the automated embryo culture and monitoring management system of the present invention. DETAILED DESCRIPTION

[0028] The following embodiments of the present invention are described in further detail in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0029] Embodiment: The present invention provides an automated embryo culture and monitoring management system, such as Figure 1 As shown, it includes an air supply module, an embryo culture module, an optical monitoring module and a liquid circuit component, and the air supply module, the embryo culture module and the optical monitoring module are electrically connected in sequence:

[0030] Gas supply module, used to provide a variety of gases required for embryo culture, and capable of online adjustment of gas concentration;

[0031] An embryo culture module, used for carrying embryos and providing a culture environment, comprises an embryo culture chamber and a plurality of gas distribution branches corresponding to the embryo culture chambers, wherein the gas distribution branches are used for delivering gas to the embryo culture chambers;

[0032] An optical monitoring module, used for monitoring the embryos in the embryo culture chamber;

[0033] The liquid circuit assembly is used to realize online automatic replacement of culture fluid and comprises a plurality of liquid distribution branches corresponding to the embryo culture chambers one by one. The liquid distribution branches are used to transport and discharge culture fluid to the embryo culture chambers.

[0034] As can be seen from the above, the gas supply module provides a variety of gases required for embryo culture, and realizes online precise adjustment of gas concentration, ensuring the stability and controllability of the embryo culture environment and improving the success rate of culture. At the same time, the optical monitoring module monitors the embryo growth status in real time, and introduces an intelligent evaluation algorithm to improve the monitoring accuracy and intelligence level. The liquid circuit component realizes online automatic replacement of the culture medium, and optimizes the replacement time in combination with the prediction algorithm, avoiding the impact of culture medium problems on embryo growth. In addition, the system also introduces a multi-objective optimization algorithm, flexibly adjusts parameters, realizes personalized embryo culture programs, and overall improves the performance and applicability of the system.

[0035] Furthermore, the gas supply module includes a multi-channel gas source supply unit and a gas mixing device, wherein the multi-channel gas source supply unit is used to provide multiple gases, and the gas mixing device is used to mix the multiple gases in a specific ratio and then transport them to the embryo culture chamber.

[0036] Furthermore, in the gas supply module, in order to achieve more accurate online regulation of gas concentration, an intelligent control algorithm is introduced. The algorithm dynamically adjusts the supply ratio and flow rate of each gas according to the gas concentration data monitored in real time in the embryo culture room to ensure that the gas concentration in the culture environment is always in the optimal state. The formula of the intelligent control algorithm includes:

[0037] C′=f(P 1 ,Q 1 ,P 2 ,Q 2 ,...,P n ,Q n );

[0038] Where C′ represents the gas concentration in the culture chamber, P 1 ,P 2 ,...,P n Indicates the supply ratio of each gas, Q 1 ,Q 2 ,...,Q nIndicates the flow rate of each gas;

[0039] The objective function of the intelligent control algorithm includes:

[0040] min(|C′-C * |);

[0041] Among them, C * is the target gas concentration.

[0042] As can be seen from the above, in the gas supply module, an intelligent control algorithm is introduced to achieve more accurate online regulation of gas concentration. The algorithm can dynamically adjust the supply ratio and flow rate of each gas according to the real-time monitoring of gas concentration data in the embryo culture room to ensure that the gas concentration in the culture environment is always in the optimal state. This not only improves the stability and controllability of the embryo culture environment, but also effectively avoids the risk of abnormal embryo development caused by gas concentration fluctuations, thereby significantly improving the success rate and development quality of embryo culture.

[0043] Furthermore, the embryo culture module also includes an embryo cover, which is used to confine the embryo in the space inside the embryo cover, and the outer wall of the embryo cover is provided with a plurality of through holes with a size that allows the culture solution to pass through but prevents the embryo from passing through.

[0044] As can be seen from the above, in the embryo culture module, an embryo cover is added and the outer wall is provided with a through hole that allows the culture fluid to pass through but prevents the embryo from passing through. The embryo cover can effectively restrain the embryo in its internal space, ensuring that the embryo will not be displaced or damaged during the culture process. At the same time, the design of the through hole allows the culture fluid to flow freely, providing sufficient nutrition and oxygen for the embryo, while preventing the embryo from passing through, avoiding direct contact and interference with the embryo during the culture process, thereby helping to maintain a good culture environment and improve the stability and success rate of embryo culture.

[0045] Furthermore, the optical monitoring module observes the embryos cultured in the embryo cover through an observation channel, and the observation channel is formed by the first glass window, the embryo cover and the second glass window facing each other in the vertical direction.

[0046] Furthermore, in the optical monitoring module, in order to improve the monitoring accuracy and intelligence level of the embryo growth status, an embryo growth status evaluation algorithm is introduced to automatically identify and extract the key growth characteristics of the embryo, including morphology, size, color, etc., so as to intelligently evaluate the growth status of the embryo. The formula of the embryo growth status evaluation algorithm includes:

[0047] S g =h(F m ,F s ,F c );

[0048] Among them, Sg Indicates embryo growth status score; F m Indicates embryo morphological characteristics; F s Indicates embryo size characteristics; F g represents the embryo color feature; h represents the function for calculating the growth status score based on the key growth characteristics of the embryo.

[0049] As can be seen above, in the optical monitoring module, an embryo growth status assessment algorithm is introduced, which can automatically identify and extract the key growth characteristics of the embryo, and obtain the embryo growth status score through intelligent calculation. This not only greatly improves the monitoring accuracy and intelligence level of the embryo growth status, but also provides researchers with more intuitive and accurate embryo growth data. Through real-time monitoring and intelligent evaluation, researchers can promptly detect abnormalities in the embryo growth process, and take effective measures to adjust and optimize, thereby improving the success rate and development quality of embryo culture.

[0050] Furthermore, the liquid circuit assembly also includes a waste liquid container connected to the ends of the liquid distribution branch pipes, for collecting the discharged culture liquid.

[0051] As can be seen from the above, in the liquid circuit assembly, a waste liquid container connected to the end of the liquid distribution branch pipe is provided to collect the discharged culture liquid. The existence of the waste liquid container ensures that the culture liquid can be effectively collected and processed during the replacement process, avoiding the problem of culture liquid leakage or environmental pollution. At the same time, this also facilitates the management and disposal of the culture liquid by scientific researchers, improves the neatness and safety of the embryo culture process, and provides a good guarantee for the healthy growth of the embryo.

[0052] Furthermore, in the fluid circuit assembly, in order to optimize the timing of replacing the culture medium, a prediction algorithm is introduced. The algorithm can predict when the culture medium needs to be replaced based on factors such as the growth rate of the embryo and the consumption of nutrients in the culture medium, so as to avoid affecting the growth of the embryo due to aging of the culture medium or insufficient nutrients. The prediction algorithm includes: assuming that the growth rate of the embryo is V G The nutrient concentration of the culture medium is C n , the threshold for changing the culture medium is T t , the prediction algorithm formula includes:

[0053] T r =g(V G ,C n ,T t );

[0054] When C n Lower than T t or V G When the temperature slows down significantly, the prediction algorithm will output the timing T of replacing the culture medium. r .

[0055] As can be seen from the above, in the liquid circuit assembly, a prediction algorithm is introduced to optimize the timing of culture medium replacement. The prediction algorithm can comprehensively consider factors such as the growth rate of the embryo and the consumption of nutrients in the culture medium, and accurately predict when the culture medium needs to be replaced. By timely replacing aging or nutrient-deficient culture medium, the adverse effects of these factors on embryo growth are effectively avoided. This not only improves the growth rate and development quality of the embryo, but also reduces the waste of resources and operating costs caused by frequent replacement of culture medium, providing strong support for the optimization of the embryo culture process.

[0056] Furthermore, the system also includes a culture medium balance chamber for storing the culture medium and delivering the culture medium to the embryo culture chamber through the liquid path component.

[0057] As can be seen from the above, the culture medium balance chamber can ensure that the culture medium maintains stable quality and temperature before delivery, providing a more suitable growth environment for the embryo. At the same time, it can also deliver culture medium in a timely and appropriate amount according to the needs of the embryo culture room, which not only meets the needs of embryo growth, but also avoids the waste of culture medium and the risk of contamination, thereby further improving the success rate and efficiency of embryo culture.

[0058] Furthermore, the system introduces a multi-objective optimization algorithm to find the optimal combination of system parameters. The formula of the multi-objective optimization algorithm includes:

[0059] v i+1 =w·v i +c 1 ·r 1 ·(p best -x i )+c 2 ·r 2 ·(g best -x i );

[0060] Among them, v i is the particle velocity, x i is the particle position, p best is the optimal position of the individual, g best is the global optimal position, w, c 1 、c 2 is the weight coefficient, r 1 、r 2 is a random number.

[0061] From the above, we can see that the multi-objective optimization algorithm is introduced to find the optimal combination of system parameters. The algorithm can dynamically adjust the system parameters and find the best balance point by comprehensively considering multiple optimization objectives, such as the accuracy of gas concentration control, the monitoring accuracy of embryo growth status, and the optimization of the timing of culture medium replacement. This intelligent parameter adjustment method not only improves the overall performance and stability of the system, but also enables the system to flexibly respond to different types of embryo culture needs and realize personalized embryo culture plans. In addition, the application of the multi-objective optimization algorithm also enhances the system's adaptive ability, helps to maintain efficient operation in a complex and changeable embryo culture environment, and further improves the success rate and efficiency of embryo culture.

[0062] Furthermore, the effects of an automated embryo culture and monitoring management system of the embodiment are compared with the currently available automated embryo culture system (comparative example), and the following table is obtained:

[0063]

[0064]

[0065] It can be seen from the above table that the automated embryo culture and monitoring management system of this embodiment is significantly superior to the currently available automated embryo culture system in terms of gas concentration control, embryo culture environment, optical monitoring accuracy, culture medium management, waste liquid treatment, system intelligence, personalized culture and overall performance, and can better meet the needs of embryo culture and improve the success rate and efficiency of embryo culture.

[0066] Working principle: By constructing an automated embryo culture and monitoring management system including a gas supply module, an embryo culture module, an optical monitoring module and a liquid circuit component, the embryo culture process is fully automated. The gas supply module provides the required gas and adjusts the concentration online, the embryo culture module provides a stable culture environment, the optical monitoring module monitors the embryo growth status in real time, and the liquid circuit component automatically replaces the culture medium. The modules work together to achieve precise control of the embryo culture environment, intelligent evaluation of the embryo growth status, and optimization of the timing of culture medium replacement through intelligent control algorithms, embryo growth status evaluation algorithms, and prediction algorithms, thereby improving the success rate and efficiency of embryo culture.

[0067] The embodiment of the present application provides an electronic device, which is applicable to the above-mentioned automated embryo culture and monitoring management system, including:

[0068] Memory, used to protect computer programs and data;

[0069] Processor, used to run system programs.

[0070] An embodiment of the present application provides a computer storage medium, which is applicable to the above-mentioned automated embryo culture and monitoring management system, and performs hierarchical confidentiality management on the above-mentioned system and data in accordance with confidentiality management requirements.

[0071] Those skilled in the art will appreciate that the embodiments of the present application may be provided as a system or a computer program product. Therefore, the present application may adopt the form of a complete hardware embodiment, a complete software embodiment, or an embodiment in combination with software and hardware. Moreover, the present application may adopt the form of a computer program product implemented on one or more computer-usable storage media (including but not limited to disk storage, CD-ROM, optical storage, etc.) that contain computer-usable program code.

[0072] The present application is described with reference to the flowcharts and / or block diagrams of the devices (systems) and computer program products according to the embodiments of the present application. It should be understood that each process and / or box in the flowchart and / or block diagram, as well as the combination of the processes and / or boxes in the flowchart and / or block diagram, can be implemented by computer program instructions. These computer program instructions can be provided to a processor of a general-purpose computer, a special-purpose computer, an embedded processor, or other programmable data processing device to generate a machine, so that the instructions executed by the processor of the computer or other programmable data processing device generate instructions for implementing the processes in the flowchart and / or block diagram. Figure 1 A process or multiple processes and / or boxes Figure 1 A device that provides the functions specified in a block or multiple blocks.

[0073] These computer program instructions may also be stored in a computer-readable memory capable of directing a computer or other programmable data processing device to operate in a specific manner, so that the instructions stored in the computer-readable memory produce an article of manufacture comprising an instruction device, which implements the process Figure 1 A process or multiple processes and / or boxes Figure 1 A function specified in one or more boxes.

[0074] These computer program instructions can also be loaded onto a computer or other programmable data processing device so that a series of operating steps are executed on the computer or other programmable device to produce a computer-implemented process, thereby providing instructions for implementing the process. Figure 1 A process or multiple processes and / or boxes Figure 1 A step that specifies a function in one or more boxes.

[0075] In a typical configuration, a computing device includes one or more processors (CPU), input / output interfaces, network interfaces, and memory.

[0076] The memory may include non-permanent memory in a computer-readable medium, random access memory (RAM) and / or non-volatile memory in the form of read-only memory (ROM) or flash RAM. The memory is an example of a computer-readable medium.

[0077] Computer readable media include permanent and non-permanent, removable and non-removable media, and can be implemented by any method or technology to store information. Information can be computer readable instructions, data structures, program modules or other data. Examples of computer storage media include, but are not limited to, phase change memory (PRAM), static random access memory (SRAM), dynamic random access memory (DRAM), other types of random access memory (RAM), read-only memory (ROM), electrically erasable programmable read-only memory (EEPROM), flash memory or other memory technology, compact disk read-only memory (CD-ROM), digital versatile disk (DVD) or other optical storage, magnetic cassettes, magnetic disk storage or other magnetic storage devices or any other non-transmission media that can be used to store information that can be accessed by a computing device. As defined herein, computer readable media does not include temporary computer readable media (transitory media), such as modulated data signals and carrier waves.

[0078] It should also be noted that the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, commodity or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, commodity or device. In the absence of more restrictions, the elements defined by the sentence "comprises a ..." do not exclude the existence of other identical elements in the process, commodity or device including the elements.

[0079] The embodiments of the present invention are provided for the purpose of illustration and description. Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in this field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. An automated embryo culture and monitoring management system, characterized in that: include: Gas supply module, used to provide a variety of gases required for embryo culture, and capable of online adjustment of gas concentration; An embryo culture module, used for carrying embryos and providing a culture environment, comprises an embryo culture chamber and a plurality of gas distribution branches corresponding to the embryo culture chambers, wherein the gas distribution branches are used for delivering gas to the embryo culture chambers; An optical monitoring module, used for monitoring the embryos in the embryo culture chamber; The liquid circuit assembly is used to realize online automatic replacement of culture fluid and comprises a plurality of liquid distribution branches corresponding to the embryo culture chambers one by one. The liquid distribution branches are used to transport and discharge culture fluid to the embryo culture chambers.

2. An automated embryo culture and monitoring management system as claimed in claim 1, characterized in that: The gas supply module includes a multi-channel gas source supply unit and a gas mixing device. The multi-channel gas source supply unit is used to provide multiple gases, and the gas mixing device is used to mix the multiple gases in a specific proportion and then transport them to the embryo culture room.

3. An automated embryo culture and monitoring management system as claimed in claim 2, characterized in that: In the gas supply module, in order to achieve more precise online regulation of gas concentration, an intelligent control algorithm is introduced. This algorithm dynamically adjusts the supply ratio and flow rate of each gas according to the gas concentration data monitored in real time in the embryo culture room.

4. An automated embryo culture and monitoring management system as claimed in claim 1, characterized in that: The embryo culture module also includes an embryo cover, which is used to confine the embryo in the space inside the embryo cover. The outer wall of the embryo cover is provided with a plurality of through holes with a size that allows the culture solution to pass through but prevents the embryo from passing through.

5. An automated embryo culture and monitoring management system as claimed in claim 1, characterized in that: The optical monitoring module observes the embryos cultured in the embryo cover through an observation channel, and the observation channel is formed by the first glass window, the embryo cover and the second glass window facing each other in the vertical direction.

6. An automated embryo culture and monitoring management system as claimed in claim 5, characterized in that: In the optical monitoring module, in order to improve the monitoring accuracy and intelligence level of embryo growth status, an embryo growth status evaluation algorithm is introduced to automatically identify and extract the key growth characteristics of the embryo.

7. An automated embryo culture and monitoring management system as claimed in claim 1, characterized in that: The liquid circuit assembly also includes a waste liquid container connected to the ends of the liquid distribution branch pipes and used for collecting the discharged culture liquid.

8. An automated embryo culture and monitoring management system as claimed in claim 7, characterized in that: In the liquid circuit component, in order to optimize the timing of replacing the culture medium, a prediction algorithm is introduced. This algorithm can predict when the culture medium needs to be replaced based on the growth rate of the embryo and the consumption of nutrients in the culture medium.

9. An automated embryo culture and monitoring management system as claimed in claim 1, characterized in that: The system also includes a culture fluid balance chamber, which is used for storing the culture fluid and transporting the culture fluid to the embryo culture chamber through the fluid path component.

10. An automated embryo culture and monitoring management system as claimed in claim 1, characterized in that: The system introduces a multi-objective optimization algorithm.

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