Strain of high polysaccharide of tremella fuciformis and application thereof

Abstract

The present application relates to a high polysaccharide strain Adel25 (Auricularia delicata), the preservation number of which is CCTCC NO: M 20242465, the strain colony is dense and neat, the growth period cycle is long, the strain is high-temperature resistant, the cultivated Auricularia delicata has high nutrient content, the ears are neat, can be turned 3-4 times, can greatly improve the yield, reduce the cost, is suitable for one-time harvesting factory large-scale production mode, and has wide market prospect.

Description

Technical Field

[0001] This invention relates to the field of biology, specifically to a high-polysaccharide strain of Auricularia auricula-judae and its applications. Background Technology

[0002] Auricularia delicata (Mont. ex Fr.) Henn., belonging to the phylum Basidiomycota, class Agaricomycetes, order Agaricales, and family Auriculariaceae, is an edible fungus used for both food and medicinal purposes. It is mainly distributed in tropical and subtropical regions of Africa, South America, and Asia. It is believed to enhance the body's immune function and has preventative and therapeutic effects on coronary heart disease. In Manipur, India, it is used as a traditional medicine to treat gastrointestinal and liver diseases.

[0003] Wrinkled wood ear mushrooms are one of the emerging edible fungi industries in my country, with large-scale production in Sichuan, Yunnan, Guizhou, Jilin, and other regions. They are a specialty edible fungus in Southwest and Northeast China. Due to their unique mushroom flavor and texture between fleshy and gelatinous, and the reticulated wrinkles on the underside of their fruiting bodies, they offer a multi-layered taste and help retain the flavor compounds of cooked ingredients, making them a highly sought-after premium edible fungus. However, there are relatively few cultivated varieties of wrinkled wood ear mushrooms; all reported varieties are derived from the domestication and systematic breeding of wild germplasm resources. There is a need for wrinkled wood ear mushroom strains with superior nutrition and texture. Summary of the Invention

[0004] The purpose of this invention is to provide the Auricularia delicata strain Adel25, accession number CCTCC NO:M 20242465.

[0005] Another object of the present invention is to provide the application of the Adel25 strain of Auricularia auricula-judae in the cultivation and breeding of Auricularia auricula-judae.

[0006] In a preferred embodiment of the present invention, the temperature during the fruiting period of the wrinkled fungus strain is 14-33℃.

[0007] In a preferred embodiment of the present invention, the wrinkled fungus obtained by cultivating the wrinkled fungus strain has a brownish-red ventral surface and a brownish-gray dorsal surface on the fresh ear pieces; and a dark brown ventral surface and a gray dorsal surface on the dried ear pieces.

[0008] In a preferred embodiment of the present invention, the dry-to-wet ratio of the wrinkled fungus is 1:8 to 1:8.5.

[0009] In a preferred embodiment of the present invention, the crude polysaccharide content of the wrinkled fungus is greater than 25%, preferably greater than 35%, and more preferably greater than 45%.

[0010] In a preferred embodiment of the present invention, the protein content of the wrinkled fungus is greater than 3%, preferably greater than 4%, and more preferably greater than 5%.

[0011] In a preferred embodiment of the present invention, the total amino acid content of the wrinkled fungus is greater than 2%, preferably greater than 3%, and more preferably greater than 4%.

[0012] In the preferred embodiment of the present invention, the wrinkled fungus strain exhibits good resistance and no specific diseases or pests have been observed.

[0013] In a preferred embodiment of the present invention, the hardness of the wrinkled fungus is greater than 300, preferably greater than 320, and more preferably greater than 340.

[0014] In a preferred embodiment of the present invention, the elasticity of the wrinkled fungus is greater than 0.5, preferably greater than 0.7, and more preferably greater than 0.9.

[0015] Unless otherwise stated, when this invention relates to percentages between liquids, the percentage is volume / volume percentage; when this invention relates to percentages between liquids and solids, the percentage is volume / weight percentage; when this invention relates to percentages between solids and liquids, the percentage is weight / volume percentage; the remainder is weight / weight percentage.

[0016] The present invention employs the following detection method:

[0017] 1. Dry-to-wet ratio: refers to the ratio of the dry weight of the ear pieces to the wet weight after soaking. The wet weight test method is to soak the dried ear pieces in water at room temperature for 4 hours, remove them and drain the water, use absorbent paper to dry the surface moisture of the ear pieces, and weigh them, referring to "NY / T 3729-2020 Guidelines for Testing the Distinctiveness (Distinguishing), Uniformity and Stability of Plant Varieties".

[0018] 2. Amino acids: GB 5009.124-2016; Proteins: GB 5009.5-2016 Method I; Crude polysaccharides: NY / T1676-2023.

[0019] 3. Hardness and Elasticity: Total Texture Analysis (TPA) was performed using a texture analyzer. The pre-test speed was 1 mm / s, the test speed was 1 mm / s, and the post-test speed was 1 mm / s. The target mode was compression (strain), the deformation was 50%, and the trigger force was 5g. A cylindrical P75 probe (75 mm in diameter) was used.

[0020] Compared with the prior art, the beneficial technical effects of the present invention include:

[0021] The wrinkled fungus strain screened in this invention has dense and uniform colonies, a long growth cycle (fruiting can occur from May to September), and is heat-resistant (fruiting can occur at a maximum temperature of 33℃). The wrinkled fungus obtained from cultivation has a high nutrient content (the polysaccharide content is 6 times that of ordinary wrinkled fungus), uniform fruiting, and can undergo 3-4 flushes, which can significantly increase the yield (twice that of ordinary wrinkled fungus). At the same time, it has a wide adaptability to raw materials (ordinary agricultural by-products can be used for cultivation), low cost, and is suitable for large-scale factory production with single-harvest processing, and has broad market prospects.

[0022] Preservation of biological materials

[0023] The wrinkled fungus Adel25 (Auricularia delicata) of this invention was deposited on November 7, 2024, with accession number CCTCC NO:M 20242465, deposited at the China Center for Type Culture Collection (CCTCC), located at Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province. Attached Figure Description

[0024] Figure 1 The front view of the wrinkled fungus mycelium in this invention;

[0025] Figure 2 This invention relates to wrinkled auricularia mycelium (cultivation strain);

[0026] Figure 3 The present invention relates to the ventral surface of the fruiting body of *Auricularia auricula-judae*. Detailed Implementation

[0027] The present invention will be described below with reference to the embodiments. However, the present invention is not limited to the embodiments.

[0028] Example 1: Screening of the *Auricularia auricula-judae* strain of the present invention

[0029] Wild *Auricularia delicata* fruiting bodies collected from Mount Emei in Sichuan Province were isolated from wild material tissue to obtain wild material strains. These strains were then purified through pure culture, and the *Auricularia delicata* strain of this invention was obtained using systematic selection methods. Morphological and ITS sequence analysis confirmed it as *Auricularia delicata* (Fr.) Henn. The basidiospores of this strain are elliptical to oval, with translucent, filamentous hyphae and clamp connections. The colonies are dense and uniform, with brown patches on the agar. Figure 1-2 ).

[0030] The strain was named Adel25 (Auricularia delicata) and was deposited on November 7, 2024, with accession number CCTCC NO:M 20242465. The depository institution is the China Center for Type Culture Collection (CCTCC), located at Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0031] Example 2: Field trial study of the wrinkled auricularia strain of the present invention

[0032] The field experiment was conducted in a greenhouse at the experimental base in Shifang City, Sichuan Province, using the wrinkled black fungus Adel25 of this invention for two consecutive years of regional variety trials.

[0033] Potato glucose agar medium (PDA): contains 200g potato, 20g glucose, 20g agar and 1000mL distilled water; take 20mL of medium and put it into a test tube (test tube diameter 20mm×200mm), sterilize at 121℃ for 30min, cool, and you will get a test tube containing PDA solid medium.

[0034] Preparation of wrinkled auricularia mycelial culture: The wrinkled auricularia strain Adel25 of the present invention was placed in a test tube (20mm in diameter × 200mm) containing PDA solid medium and cultured at 25℃ for 15 days to obtain the culture.

[0035] Cultivation bags (200mm×480mm×0.28mm) containing 1000g of cultivation material: The cultivation material, by weight ratio, consists of 330g sawdust, 100g cottonseed hulls, 300g corn cobs, 200g wheat bran, 20g corn flour, 40g lime, 10g gypsum, and 600g water. The cultivation bags are sterilized at 121℃ for 2.5 hours and then cooled before use.

[0036] The experimental method was as follows: tiered bag cultivation, with no fewer than 60 bags and 3 replicates. Bags were prepared in December, and fruiting bodies emerged from May to September of the following year. Cultivation bags inoculated with 10g of *Auricularia auricula-judae* mycelial culture per bag were placed in a cultivation room at 18-26℃ in the dark for 30 days for fruiting management. During fruiting, the temperature was maintained between 14℃ and 33℃, and the relative humidity inside the cultivation room was kept at 85%–95%. Light intensity should not exceed 310 lux on sunny days. Good ventilation was essential; the greenhouse doors were opened for ventilation in the morning and evening for 15–30 minutes per day. Fruiting bodies were ready for harvest when the edges of the fruiting bodies slightly curled upwards.

[0037] Cultivation results showed that the yield was 87.1 g / bag dry and 696.8 g / bag fresh. Mature fruiting bodies are single-lobed and clustered, with smooth edges. Fresh auricles have vein-like wrinkles on the ventral side, are brownish-red, and brownish-gray on the dorsal side; dried auricles are dark brown on the ventral side and gray on the dorsal side. Figure 3When the ears emerge from both ends during long-stick cultivation in the greenhouse, the fresh ear pieces should be 60.3–66.4 mm long, 71.3–76.8 mm wide, and 2.0–3.4 mm thick, with a dry-to-wet ratio of 1:8.

[0038] Routine nutritional analysis of mature dried fruiting bodies revealed that per 100 grams, there were 49.5 g of crude polysaccharides, 5.32 g of protein, and 4.1 g of total amino acids. The texture was crisp and elastic, with a hardness value of 344.12 ± 22.058 and an elasticity value of 0.91 ± 0.013.

[0039] Furthermore, statistics showed that the contamination rate of the wrinkled fungus mycelium cultivation spawn was only 3.5%, and the contaminating fungus was a common edible fungus contaminant, *Vibrio vulgaris*, caused by damage to the cultivation bags. No specific diseases or pests were observed during the fruiting period, indicating that the wrinkled fungus of this invention has strong resistance to contamination.

[0040] The above description of specific embodiments of the present invention does not limit the present invention. Those skilled in the art can make various changes or modifications based on the present invention, and as long as they do not depart from the spirit of the present invention, they should all fall within the scope of protection of the claims of the present invention.

Claims

1. Wrinkled wood ear fungus ( Auricularia delicata (Strain Adel25, accession number CCTCC NO: M 20242465.) 2. The application of the wrinkled fungus strain as described in claim 1 in the cultivation and breeding of wrinkled fungus, wherein the crude polysaccharide content of the wrinkled fungus is greater than 35%.

3. In the application described in claim 2, the temperature during the fruiting period of the wrinkled fungus strain is 14-33℃.

4. In the application described in claim 2, the wrinkled fungus obtained by cultivating the wrinkled fungus strain has a brownish-red ventral surface and a brownish-gray dorsal surface on the fresh ear pieces; and a dark brown ventral surface and a gray dorsal surface on the dried ear pieces.

5. In the application described in claim 2, the dry-to-wet ratio of the wrinkled fungus is 1:8 to 1:8.

5.

6. In the application described in claim 2, the crude polysaccharide content of the wrinkled fungus is greater than 45%.

7. The application as described in claim 2, wherein the protein content of the wrinkled fungus is greater than 3%.

8. In the application described in claim 7, the protein content of the wrinkled fungus is greater than 4%.

9. The application as described in claim 8, wherein the protein content of the wrinkled fungus is greater than 5%.

10. In the application described in claim 2, the total amino acid content of the wrinkled fungus is greater than 2%.

11. The application as described in claim 10, wherein the total amino acid content of the wrinkled fungus is greater than 3%.

12. The application as described in claim 11, wherein the total amino acid content of the wrinkled fungus is greater than 4%.

13. In the application described in claim 2, the hardness of the wrinkled fungus is greater than 300.

14. The application as described in claim 13, wherein the hardness of the wrinkled fungus is greater than 320.

15. The application as described in claim 14, wherein the hardness of the wrinkled fungus is greater than 340.

16. In the application as described in claim 2, the elasticity of the wrinkled fungus is greater than 0.

5.

17. The application as described in claim 16, wherein the elasticity of the wrinkled fungus is greater than 0.

7.

18. The application as described in claim 17, wherein the elasticity of the wrinkled fungus is greater than 0.9.

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