Polypeptide variants

Abstract

The present invention relates to polypeptide variants and methods for obtaining variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Description

[0001] This application is a divisional application of the Chinese invention patent application with the application date of April 6, 2020, application number: 202080025024.6, and invention name: "Polypeptide Variant".

[0002] References to sequence listings

[0003] This application contains a Sequence Listing in computer readable form, which is incorporated herein by reference. Technical Field

[0004] The present invention relates to novel dispersin variants having improved properties relative to the parent dispersin protein, which may include one or more of the following: wash performance, stability, such as detergent and / or storage stability. The variants of the present invention are suitable for use in cleaning processes and detergent compositions, such as laundry compositions and dishwashing compositions (including hand and automatic dishwashing compositions). The present invention also relates to isolated DNA sequences encoding these variants, expression vectors, host cells, and methods for producing and using the dispersin variants of the present invention. Background Art

[0005] Enzymes have been used in detergents for decades, the most commercially relevant being proteases and amylases, which are effective in removing protein- and starch-related soils, respectively. However, most soils associated with household care are complex mixtures of various organic substances. Hard surfaces and fabrics are exposed to skin debris, such as dead cells, sweat, sebum, pollution, and stains originating from microorganisms from the environment and from, for example, the human body. Complex organic stains, such as biofouling from human skin, such as dead skin cells, sweat, sebum, and in particular underarm soil, are composed of different substances, such as proteins, starches, greases, but also, for example, polysaccharides. Therefore, different enzyme activities are required for stain removal, depending on the specific stain being targeted.

[0006] To be useful in cleaning processes, dispersed proteins such as laundry enzymes need to be stable in detergent compositions and compatible with standard detergent ingredients such as surfactants, builders, bleaches and the like.

[0007] The present invention provides such enzymes which are suitable for use in detergents, for example for improved laundry cleaning and washing / dishwashing machines. Summary of the Invention

[0008] The present invention relates to dispersin variants which are particularly useful in detergents and cleaning processes such as laundry and dishwashing.

[0009] One aspect of the invention relates to a dispersin variant comprising an alteration, preferably a substitution, at one or more positions corresponding to one or more of the following positions: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 115, 116, 117, 118, 119, 210, 211, 219, 220, 221, 222, 223 4, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 1 88, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 or 323, wherein the disperse protein variant has hexosaminidase activity, preferably β-1,6N-acetylglucosaminidase activity, and wherein the variant has increased stability, preferably thermostability, as measured by half-life improvement factor (HIF), compared to the disperse protein having SEQ ID NO: 1.

[0010] One aspect of the invention relates to a dispersin variant comprising an alteration, preferably a substitution, at one or more positions corresponding to positions 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 115, 116, 117, 118, 119, 210, 211, 219, 220, 221, 222, 223 4, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187 7, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 27 1, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein the dispersin variant has β-1,6N-acetylglucosaminidase activity, wherein the dispersin variant is the same as SEQ The polypeptide shown in ID NO: 1 has at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity, and wherein the variant has increased stability, preferably thermostability, as measured by half-life improvement factor (HIF), compared to the dispersed protein of SEQ ID NO: 1.

[0011] One aspect of the invention pertains to dispersin variants comprising at least one alteration selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N4 5L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N6 2H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S7 2M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L9 0F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V1 06E, V106H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107 C. K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D 111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y11 6N, Y116R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G,E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H、Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A2 71D, A271G, H272D, H272I, H272M, H272P, H272V, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276 G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y282E, Y282N, Y281R, H283I, A284I, A284L, A284N, A28 4P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R , T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K3 8L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein these positions correspond to SEQ 1, wherein the dispersin variant has hexosaminidase activity, preferably β-1,6N-acetylglucosaminidase activity, preferably wherein the alteration at one or more positions results in a dispersin variant having increased stability, preferably thermostability, as measured by an improvement factor HIF of at least 1.1 compared to the polypeptide shown in SEQ ID NO: 1, and preferably wherein the dispersin variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 90%, at least 100%, at least 110%, at least 120%, at least 130%, at least 140%, at least 150%, at least 160%, at least 170%, at least 180%, at least 190%, at least 200%, at least 210%, at least 220%, at least 230%, at least 240%, at least 250%, at least 260%, at least 270%, at least 280%, at least 290%, at least 300%, at least 310%, at least 320%, at least 330%, at least 340%, at least 350%, at least 360%, at least 370%, at least 380%, at least 390%, at least 400%, at least 410%, at least 420%, at least 430%, at least 440%, at least 450%, at least 460%, at least 470%, at least 480%, at least 490%, at least 500%, at least 510%, at least 520%, at least 530%, at least 540%, at least 550%, at least 560%, at least 570%, at least 580%, at least 590%, at least 600%, at least 600%, at least 6At least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity.

[0012] One aspect of the present invention relates to a method for obtaining a dispersin variant, the method comprising:

[0013] a) introducing into a dispersin parent having hexosaminidase, preferably β-1,6-N-acetylglucosaminidase activity, an alteration, preferably a substitution, at one or more positions corresponding to: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110 of the polypeptide of SEQ ID NO: 1 、111、113、114、116、117、118、119、120、122、123、124、125、126、127、128、131、135、138、139、140、142、145、147、148、149、150、151、152、163、164、167、168、170、171、173、174、175、177、1 78, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261 1, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, or 323,

[0014] b) and recovering the variant.

[0015] One aspect of the present invention relates to a method for obtaining a dispersin variant, the method comprising:

[0016] a) introducing into the disperse protein parent having 1,6-N-acetylglucosaminidase activity

[0017] The alteration, preferably substitution, at one or more positions corresponding to the following positions: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110 of the polypeptide of SEQ ID NO: 1. 、111、113、114、116、117、118、119、120、122、123、124、125、126、127、128、131、135、138、139、140、142、145、147、148、149、150、151、152、163、164、167、168、170、171、173、174、175、177、1 78, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261 1, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, and 323.

[0018] b) and recovering the variant.

[0019] One aspect of the present invention relates to a composition, preferably a cleaning composition, comprising a variant according to the invention.

[0020] One aspect of the present invention relates to a method of cleaning an item comprising a) adding a dispersed protein variant according to the present invention to a cleaning composition and b) washing the item with the composition, wherein the item is a textile or a hard surface.

[0021] One aspect of the present invention relates to a method for finding improved polypeptide variants, the method comprising the steps of:

[0022] a) generating diversity at selected, preferably all, polypeptide positions, thereby producing polypeptide variants (V L ) library, and

[0023] b) Selecting a sublibrary with improved variants (V LSub ).

[0024] The above method preferably comprises subjecting some subset of variants to one or more stress conditions and then subjecting the reference disperse protein variant to no stress.In this way the subsets can be compared and improved variants selected.

[0025] One aspect of the present invention relates to a method for finding improved polypeptide variants, the method comprising the steps of:

[0026] a) generating diversity at selected, preferably all, polypeptide positions, thereby producing polypeptide variants (V L )'s library,

[0027] b) polypeptide variant (V L ) is exposed to one or more stress conditions suitable for measuring the property of interest, thereby generating stress V LS+n and unstressed V LUS library;

[0028] c) Compare the stress V LS and unstressed V LUS library; and

[0029] d) Selecting a sublibrary with improved variants (V LSub ).

[0030] definition

[0031] The term "adjunct material" means any liquid, solid or gaseous material selected for a particular type of detergent composition and product form (e.g., liquid, granule, powder, rod, paste, spray, tablet, gel or foam composition) desired, which is also preferably compatible with the dispersoid variant enzyme used in the composition. In some respects, the granular composition is in a "compressed" form, while in other respects, the liquid composition is in a "concentrated" form.

[0032] The term "allelic variant" means any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation occurs naturally through mutation and can lead to polymorphism within a population. Gene mutations can be silent (no change in the encoded polypeptide) or can encode a polypeptide with an altered amino acid sequence. An allelic variant of a polypeptide is a polypeptide encoded by an allelic variant of a gene.

[0033] The term "biofilm" means that on a surface, for example, textiles, tableware or hard surfaces, cells adhere to each other in any group of microorganisms. These adherent cells are usually embedded in the autogenous matrix of extracellular polymeric substances (EPS). Biofilm EPS is a polymer mass generally composed of extracellular DNA, proteins and polysaccharides. Biofilm can be formed on living or non-living surfaces. The microbial cells grown in biofilms are physiologically different from the planktonic cells of the same organism (by contrast, planktonic cells are single cells that can float or float in a liquid medium). The bacteria living in biofilms usually have significantly different characteristics from the free-floating bacteria of the same species, because the dense and protected environment of the film allows them to collaborate and interact in different ways. A benefit of this environment is to increase resistance to detergents and antibiotics, because the dense extracellular matrix and the outer layer of the cell protect the inside of the community. Bacteria that produce laundry biofilms can be found in the following species: Acinetobacter sp., Aeromicrobium sp., Brevundimonas sp., Microbacterium sp., Micrococcus luteus, Pseudomonas sp., Staphylococcus epidermidis, and Stenotrophomonas sp.

[0034] The term "biofouling" means stains composed of several types of organic material, such as proteins, starches, greases, but also e.g. polysaccharides.

[0035] The term "cDNA" means a DNA molecule that can be prepared by reverse transcription from a mature, spliced ​​mRNA molecule obtained from a prokaryotic or eukaryotic cell. cDNA lacks intron sequences that may be present in the corresponding genomic DNA. The initial primary RNA transcript is a precursor to mRNA that is processed through a series of steps, including splicing, before appearing as mature, spliced ​​mRNA.

[0036] The term "clade" means a group of polypeptides that are grouped together based on homology characteristics traced back to a common ancestor. A polypeptide clade can be viewed as a phylogenetic tree, and a clade is a group of polypeptides consisting of a common ancestor and all its direct descendants. Polypeptides that form a clade such as shown in a phylogenetic tree often share common characteristics and are also more closely related in function than other polypeptides that are not in the clade.

[0037] The term "coding sequence" means a polynucleotide that directly specifies the amino acid sequence of its polypeptide product. The boundaries of the coding sequence are generally determined by an open reading frame, which usually begins with the ATG start codon or an alternative start codon (such as GTG and TTG) and ends with a stop codon (such as TAA, TAG and TGA). The coding sequence can be a DNA, cDNA, synthetic, or recombinant polynucleotide.

[0038] The term "control sequence" means all components necessary for the expression of a polynucleotide encoding a variant of the present invention. Each control sequence may be native or foreign to the polynucleotide encoding the variant, or may be native or foreign to each other. Such control sequences include, but are not limited to, a leader sequence, a polyadenylation sequence, a propeptide sequence, a promoter, a signal peptide sequence, and a transcription terminator. At a minimum, a control sequence includes a promoter, and transcriptional and translational stop signals. These control sequences may be provided with linkers for the introduction of specific restriction enzyme sites that facilitate ligation of these control sequences to the coding region of the polynucleotide encoding the variant.

[0039] By the term "deep-cleaning" is meant the destruction or removal of components of the organic matter (eg, biofouling or biofilm and EPS, such as polysaccharides, PNAGs, proteins, DNA, dirt) or other components present in the organic matter.

[0040] Unless otherwise indicated, the term "detergent composition" includes general-purpose or heavy-duty detergents in granular or powder form, especially cleaning detergents; general-purpose detergents in liquid, gel or paste form, especially the so-called heavy-duty liquid (HDL) type; liquid delicate fabric detergents; hand dishwashing detergents or light-duty dishwashing detergents, especially those of the high-sudsing type; machine dishwashing detergents, including different tablets, granules, liquids and rinse aid types for use in homes and institutions; liquid cleaners and disinfectants, including antibacterial hand wash types, cleaning bars, soap bars, mouthwashes, denture cleaners, car or carpet shampoos, bathroom cleaners; shampoos and hair rinses; shower gels, foam baths; metal cleaners; as well as cleaning aids (such as bleach additives) and "stain-sticks" or pre-treatment types. The terms "detergent composition" and "detergent formulation" are used with respect to mixtures in a wash medium intended for cleaning soiled objects. In some aspects, the term is used with respect to washing fabrics and / or clothes (e.g., "laundry detergent"). In alternative aspects, the term refers to other detergents, such as those used to clean dishes, cutlery, etc. (e.g., "dishwashing detergents"). It is not intended that the invention be limited to any particular detergent formulation or composition. The term "detergent composition" is not intended to be limited to compositions containing surfactants. It is intended that, except in accordance with the variants of the present invention, the term encompasses detergents that may contain, for example, surfactants, builders, chelators or chelating agents, bleach systems or bleach components, polymers, fabric conditioners, foam boosters, suds suppressors, dyes, perfumes, tarnish inhibitors, optical brighteners, bactericides, fungicides, soil suspending agents, corrosion inhibitors, enzyme inhibitors or stabilizers, enzyme activators, one or more transferases, hydrolases, oxidoreductases, bluing agents and fluorescent dyes, antioxidants, and solubilizing agents.

[0041] The term "dispersin" and the abbreviation "Dsp" or "Disp" are polypeptides with hexosaminidase activity, EC 3.2.1.-, found, for example, in biofilms, EPS, cell debris and other biofouling, which catalyze the hydrolysis of β-1,6-glycosidic bonds of N-acetyl-glucosamine polymers (poly-N-acetylglucosamine, PNAG). Thus, dispersin is an enzyme with β-1,6N-acetylglucosamine glycosidase activity or poly-β-1,6-N-acetylglucosamine (PNAG) activity. For the purposes of the present invention, dispersin activity, i.e., β-1,6N-acetylglucosamine glycosidase activity, is determined according to the procedure described in the activity assay of Example 3. In some aspects, the dispersin variants of the present invention have improved dispersin activity compared to the parent dispersin. In some aspects, the dispersin variants of the invention have at least 100%, e.g., at least 110%, at least 120%, at least 130%, at least 140%, at least 150%, at least 160%, at least 170%, at least 180%, at least 190%, or at least 200% of the dispersin activity compared to the polypeptide set forth in SEQ ID NO: 1, or e.g., from 100% to 200% of the dispersin activity compared to the polypeptide set forth in SEQ ID NO: 1.

[0042] The term "effective amount of an enzyme" refers to the amount of enzyme necessary to achieve the desired enzymatic activity in a particular application, e.g., in a defined detergent composition. Such an effective amount is readily determined by one of ordinary skill in the art and is based on many factors, such as the specific enzyme being used, the cleaning application, the specific composition of the detergent composition, and whether a liquid or dry (e.g., granular, bar) composition is desired. The term "effective amount" of a dispersoid variant refers to the amount of the aforementioned dispersoid variant that achieves the desired level of enzymatic activity (e.g., in a defined detergent composition).

[0043] The term "expression" includes any step involved in the production of the variant including, but not limited to, transcription, post-transcriptional modification, translation, post-translational modification, and secretion.

[0044] The term "expression vector" means a linear or circular DNA molecule comprising a polynucleotide encoding a variant and operably linked to additional nucleotides that provide for its expression.

[0045] The term "fabric" encompasses any textile material. Thus, it is intended that the term encompass garments, as well as fabrics, yarns, fibers, nonwoven materials, natural materials, synthetic materials, and any other textile material.

[0046] The term "high detergent concentration" system includes detergents in which greater than about 2000 ppm of detergent components are present in the wash water. European detergents are generally considered to be high detergent concentration systems.

[0047] The term "host cell" means any cell type that is susceptible to transformation, transfection, transduction, etc. with a nucleic acid construct or expression vector comprising a polynucleotide of the present invention. The term "host cell" encompasses any progeny of a parent cell that is not identical to the parent cell due to mutations that occur during replication.

[0048] The term "improved properties" means features associated with a variant that are improved compared to the parent and / or compared to a dispersed protein having SEQ ID NO: 1, or compared to a dispersed protein having the same amino acid sequence as the variant but without changes at two or more of the specified positions. Such improved properties include, but are not limited to, stability, such as detergent stability, washing performance (e.g., a deep cleaning effect, and the deep cleaning effect may include, but is not limited to, a degumming effect (some organic materials such as EPS are sticky, i.e., glue-like and cause dirt to stick to the material)), increased whiteness, reduced malodor and redeposition. Improved properties also include improved stability in the presence of certain enzymes (e.g., protease stability) or substances (e.g., stability in the presence of sulfites (e.g., sulfite stability)).

[0049] The term "improved dispersin activity" is defined herein as an altered dispersin activity, for example by increased catalysis of the β-1,6-glycosidic bond of an N-acetyl-glucosamine polymer (poly-N-acetylglucosamine, PNAG), i.e., a dispersin variant exhibits a change in activity relative to (or compared to) the activity of the parent dispersin variant (e.g., compared to a dispersin having SEQ ID NO: 1 or compared to a dispersin variant having the same amino acid sequence but without an alteration at one or more of the specified positions).

[0050] The term "low detergent concentration" system includes detergents in which less than about 800 ppm of detergent components are present in the wash water. Asian (eg, Japanese) detergents are typically considered to be low detergent concentration systems.

[0051] The term "improved washing performance" includes, but is not limited to, the term "deep cleaning effect". Improved properties, such as compared to a disperse protein parent (the disperse protein shown in SEQ ID NO: 1) or compared to a disperse protein having the same amino acid sequence as the variant but not having a change at one or more of the specified positions, measure the deep cleaning performance of disperse protein variants according to the present invention. Improved performance, such as deep cleaning performance, can be expressed as the reflectance value of a small piece of cloth sample stained. After washing and rinsing, the small piece of cloth sample is spread out and allowed to air dry overnight at room temperature. All washed small pieces of cloth samples are assessed the next day after washing. Light reflectance evaluation of small pieces of cloth samples is performed using a Macbeth Color Eye 7000 reflectance spectrophotometer with a very small aperture. Measured under the condition of no UV in the incident light, and the reflectance at 460nm is extracted. A positive reaction indicates that dirt is removed.

[0052] The term "laundry" refers to both domestic and industrial laundry and means the process of treating textiles with a solution containing the cleaning or detergent composition of the present invention. The laundry process can be carried out, for example, using, for example, a domestic or industrial washing machine or can be carried out manually.

[0053] The term "mature polypeptide" means a polypeptide in its final form after translation and any post-translational modifications (e.g., N-terminal processing, C-terminal truncation, glycosylation, phosphorylation, etc.). In some aspects, the mature polypeptide is amino acids 1 to 324 of SEQ ID NO: 1. The N-terminus of the mature polypeptide used in accordance with the present invention can be experimentally confirmed based on EDMAN N-terminal sequencing data and complete MS data. It is known in the art that a host cell can produce a mixture of two or more different mature polypeptides (i.e., having different C-terminal and / or N-terminal amino acids) expressed from the same polynucleotide.

[0054] The term "medium detergent concentration" system includes detergents in which between about 800 ppm and about 2000 ppm of detergent components are present in the wash water.North American detergents are generally considered to be medium detergent concentration systems.

[0055] The term "nucleic acid construct" means a single-stranded or double-stranded nucleic acid molecule isolated from a naturally occurring gene, or modified in a manner that does not otherwise exist in nature to contain a nucleic acid fragment, or synthesized. When the nucleic acid construct contains the control sequences required for expression of a coding sequence of the present invention, the term nucleic acid construct is synonymous with the term "expression cassette."

[0056] The term "non-fabric detergent composition" includes non-textile surface detergent compositions including, but not limited to, compositions used for hard surface cleaning, such as dishwashing detergent compositions (including hand dishwashing compositions), oral compositions, denture compositions, and personal cleansing compositions.

[0057] The term "operably linked" means a configuration in which a control sequence is placed at an appropriate position relative to the coding sequence of a polynucleotide such that the control sequence directs the expression of the coding sequence.

[0058] The terms "parent" dispersin, dispersin parent, or precursor dispersin are used interchangeably. In the context of the present invention, "parent dispersin" is understood to mean a dispersin in which at least one change has been made in the amino acid sequence to produce a dispersin variant having an amino acid sequence that is less than 100% identical to the dispersin sequence in which the change was made, i.e., the parent dispersin. Thus, the parent is a dispersin having the same amino acid sequence as the variant but without changes at one or more of the specified positions. It will be understood that the expression "having the same amino acid sequence" in this context is relative to 100% sequence identity. In specific aspects, the dispersin parent is a dispersin having at least 60%, at least 61%, at least 62%, at least 63%, at least 64%, at least 65%, at least 66%, at least 67%, at least 68%, at least 69%, at least 70%, at least 72%, at least 73%, at least 74%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, e.g., at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6 or 100% identity to the polypeptide shown in SEQ ID NO: 1.

[0059] The term "relevant washing conditions" as used herein indicates the conditions actually used in household use in the detergent market segment, in particular wash temperature, time, wash mechanics, detergent concentration, detergent type and water hardness.

[0060] The term "stability" includes storage stability and stability during use (e.g., during washing) and reflects the stability of the dispersed protein variant according to the present invention as a function of time, e.g., how much activity is retained when the dispersed protein variant is placed in a solution, especially in a detergent solution. Stability is affected by many factors, such as pH, temperature, detergent composition, e.g., the amount of builder, surfactant, etc. As described in Examples 2a, 2b, 3, 4, 5, and 6, it is possible to improve the RA (residual activity), T m (melting temperature) or T 1 / 2 (half-life) is used to measure dispersoid stability. The term "improved stability" or "increased stability" is defined herein as the stability of a variant dispersoid showing increased stability in solution relative to the parent dispersoid and / or relative to the stability of SEQ ID NO: 1. "Improved stability" and "increased stability" include detergent stability. The term "detergent stability" or "improved detergent stability" is defined as improved stability in a detergent (e.g., a cleaning composition) compared to the parent dispersoid. The stability of the variant dispersoid can be determined by RAR (residual activity ratio), HIF (half-life improvement factor), or T as described in Examples 2a, 2b, 3, 4, 5, and 6. m to measure the improvements of disperse protein variants.

[0061] The term "textile" refers to woven fabrics, and staple fibers and filaments suitable for conversion into or use as yarn, weaving, knitting, and non-woven fabrics. The term encompasses yarns made from natural and synthetic (e.g., manufactured) fibers. The term "textile material" is a general term for fibers, yarn intermediates, yarns, fabrics, and products (e.g., clothing and other articles) made from fabrics.

[0062] The term "transcription terminator" is used to refer to a segment of gene sequence that marks the end of a gene or operator on genomic DNA for transcription.

[0063] The term "variant" means a polypeptide comprising an alteration at one or more (e.g., several) positions compared to a parent or reference polypeptide. The alteration may be a substitution, insertion, or deletion. Substitution means that the amino acid occupying a position is replaced by a different amino acid, deletion means removing the amino acid occupying a position, and insertion means adding an amino acid adjacent to the amino acid occupying a position, e.g., 1 to 10 amino acids, preferably 1 to 3 amino acids. The term "dispersin variant" means a polypeptide having hexosaminidase, preferably β-1,6N-acetylglucosaminidase activity or having activity on poly-β-1,6-N-acetylglucosamine (PNAG), and comprising an alteration (i.e., substitution, insertion, and / or deletion) at one or more (or one or several) positions compared to a parent disperse protein (e.g., compared to SEQ ID NO: 1). The term "dispersin activity" means a polypeptide having hexosaminidase, preferably β-1,6N-acetylglucosaminidase activity or activity towards poly-β-1,6-N-acetylglucosamine (PNAG). Variants of the present invention, such as dispersin variants, preferably have at least 20%, for example, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, or at least 100% of the dispersin activity of the polypeptide shown in SEQ ID NO: 1.

[0064] As used herein, the term "water hardness" or "degree of hardness" or "dH" or "°dH" refers to German degrees of hardness. One degree is defined as 10 mg calcium oxide per liter of water.

[0065] The term "wild-type dispersin" means dispersin expressed by a naturally occurring organism, such as a fungus, bacterium, archaea, yeast, plant, or animal found in nature.

[0066] Variant naming conventions

[0067] For the purposes of the present invention, the polypeptide disclosed in SEQ ID NO: 1 is used to determine the corresponding amino acid residue in another disperse protein. The amino acid sequence of another disperse protein is aligned with the polypeptide disclosed in SEQ ID NO: 1, and based on this alignment, the amino acid position number corresponding to any amino acid residue in the polypeptide disclosed in SEQ ID NO: 1 is determined using, for example, the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol. 48: 443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277) (preferably version 5.0.0 or later). The parameters used are a gap opening penalty of 10, a gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix.

[0068] The identification of the corresponding amino acid residue in another disperse protein can be determined by alignment of multiple polypeptide sequences using several computer programs using their corresponding default parameters, including but not limited to MUSCLE (Multiple Sequence Comparison by Log-Expectation; Version 3.5 or later; Edgar, 2004, Nucleic Acids Research 32:1792-1797), MAFFT (Version 6.857 or later; Katoh and Kuma, 2002, Nucleic Acids Research 30:3059-3066; Katoh et al., 2005, Nucleic Acids Research 33:511-518; Katoh and Toh, 2007, Bioinformatics 23:372-374; Katoh et al., 2009, Methods in Molecular Biology, 19 ... Biology 537:39-64; Katoh and Toh, 2010, Bioinformatics 26:1899-1900), and EMBOSS EMMA using ClustalW (version 1.83 or later; Thompson et al., 1994, Nucleic Acids Research 22:4673-4680).

[0069] When another enzyme deviates from the polypeptide of, for example, SEQ ID NO: 1 so that traditional sequence-based comparison methods cannot detect their relationship (Lindahl and Elofsson, 2000, J. Mol. Biol. 295: 613-615), other pairwise sequence comparison algorithms can be used. Higher sensitivity in sequence-based searches can be obtained using search programs that use probabilistic representations (profiles) of families of polypeptides to search databases. For example, the PSI-BLAST program generates multiple profiles through an iterative database search process and is capable of detecting distant homologs (Atschul et al., 1997, Nucleic Acids Res. 25: 3389-3402). Even higher sensitivity can be achieved if the family or superfamily of polypeptides has one or more representatives in the protein structure database. Programs such as GenTHREADER (Jones, 1999, J. Mol. Biol. 287:797-815; McGuffin and Jones, 2003, Bioinformatics 19:874-881) use information from a variety of sources (PSI-BLAST, secondary structure predictions, structural alignment profiles, and solvation potential) as input to a neural network that predicts the structural fold of a query sequence. Similarly, the method of Gough et al., 2000, J. Mol. Biol. 313:903-919 can be used to align sequences of unknown structure with superfamily models present in the SCOP database. These alignments can, in turn, be used to generate homology models for polypeptides, and the accuracy of such models can be assessed using a variety of tools developed for this purpose.

[0070] For proteins of known structure, there are several tools and resources that can be used to retrieve and produce structural alignments. For example, the SCOP superfamily of proteins has been structurally aligned, and those alignments are accessible and downloadable. Multiple algorithms such as distance alignment matrices (Holm and Sander, 1998, Proteins [protein] 33:88-96) or combined extensions (Shindyalov and Bourne, 1998, Protein Engineering [protein engineering] 11:739-747) can be used to compare two or more protein structures, and the implementation of these algorithms can be used in addition to query the structural database with the structure of interest to find possible structural homologues (for example, Holm and Park, 2000, Bioinformatics [biological information science] 16:566-567).

[0071] Since different amino acids may be present at a given position depending on the variant parent selected, amino acid positions are indicated by #1, #2, etc. in the following definitions. In describing the variants of the present invention, the nomenclature described below has been adapted for ease of reference. Accepted IUPAC single-letter or three-letter amino acid abbreviations are used.

[0072] replace : For amino acid substitutions, the following nomenclature is used: original amino acid, position, substituted amino acid. Thus, a substitution of valine at position #1 with alanine is indicated as "Val#1Ala" or "V#1A." Multiple mutations can be separated by a plus sign ("+") or a comma (,), for example, "Val#1Ala+"Pro#2Gly" or V#1A,P#2G represent substitutions of valine (V) and proline (P) at positions #1 and #2 with alanine (A) and glycine (G), respectively. If more than one amino acid can be substituted in a given position, the amino acids can be listed in brackets, such as [X] or {X}. Thus, if both Trp and Lys can be substituted instead of the amino acid occupying position #1, this can be represented as X#1{W,K}, X#1[W,K] or X#1[W / K], where X represents the amino acid residue at that position present in the parent disperse protein (e.g., disperse protein such as shown in SEQ ID NO: 1 or a disperse protein having at least 60% identity thereto). In some cases, these variants can be represented as #1{W,K} or X#2P, emphasizing that the amino acids to be substituted vary depending on the parent. For convenience, when SEQ ID NO: 1 is used, the amino acids to be substituted vary. When NO: 1 is used for numbering, the amino acid in the corresponding position in SEQ ID NO: 1 (e.g., D2V) is used to represent it. However, it will be clear to those skilled in the art that dispersin variants comprising D2V are not limited to the parent dispersin having a "D" (aspartic acid) at the position corresponding to position 2 of SEQ ID NO: 1. In parent dispersin having, for example, asparagine at position 2, one skilled in the art would designate the translational mutation as D2V to N2V. In cases where the parent dispersin has a valine at position 1, one skilled in the art would recognize that the parent dispersin has not changed at this position. The same applies to deletions and insertions described below.

[0073] Missing : For amino acid deletions, the following nomenclature is used: original amino acid, position, * Thus, a deletion of valine at position #1 is represented as "Val#1*" or "V#1*." Multiple deletions can be separated by a plus sign ("+") or a comma, for example, "Val#1*+Pro#2*" or "V#1*,P#2*."

[0074] insertInsertion of an additional amino acid residue, such as, for example, the insertion of a lysine after Val#1, can be represented as: Val#1ValLys or V#1VK. Alternatively, insertion of an additional amino acid residue, such as the insertion of a lysine after V#1, can be represented as: *#1aK. When more than one amino acid residue is inserted, such as, for example, the insertion of Lys and Ala after #1, such insertion can be represented as: Val#1ValLysGly or V#1VKG. In such cases, the inserted amino acid residue(s) can also be numbered by adding lowercase letters to the amino acid residue position number preceding the inserted amino acid residue(s), in this example: *#1aK *#1bG.

[0075] Various changes : Variants containing multiple alterations can be separated by a plus sign ("+") or by a comma (,), for example, "Val#1Trp+Pro#2Gly" or "V#1W,P#2G" represent that valine and proline at positions #1 and #2 are replaced by tryptophan and glycine, respectively, as described above.

[0076] Different changes: When different changes can be introduced at one position, these are separated by commas, e.g., "Val#1Trp,Lys" or V#1W,K represents a substitution of valine at position #1 by tryptophan or lysine. Thus, "Val#1Trp,Lys+Pro#2Asp" represents the following variants: "Val#1Trp+Pro#2Asp", "Val#1Lys+Pro#2Asp" or V#1W,K+P#2D. In the context of the present invention, the term "substitution group" is a variant comprising more than one mutation compared to a parent or reference enzyme, e.g., the substitution group D2V+Q3L (compared to SEQ ID NO: 1) is a variant of SEQ ID NO: 1 comprising two mutations, D2V+Q3L, compared to SEQ ID NO: 1.

[0077] When changes, such as mutations in a substitution group, are separated by commas or plus signs "+", this means that all changes in the group are present and the selection is between the changes (change groups). For example, "comprising one or more of the following substitution groups: Q3I+A49W, Q3I+N59E, Q3I+S163P, Q3I+S186R, Q3I+Q215K, Q3I+S225G, Q3I+N227T, Q3I+N252P, Q3I+N267T, Q3I+F276A, Q3I+Y281P, Q3I+K308Q, Q3I+K308E, Q3I+K309E, Q3I+K312Q, Q3I+K312E..."

[0078] Means to choose between each substitution group separated by commas.

[0079] The term parent enzyme includes terms such as reference enzyme, backbone enzyme or starting enzyme and is used to refer to the enzyme to which the mutation (e.g. substitution) is made. These terms can be used interchangeably.

[0080] Specific nomenclature used for clades

[0081] For the purposes of the present invention, the designation [IV] or [I / V] means that the amino acid at that position can be isoleucine (Ile, I) or valine (Val, V). Similarly, for other combinations as described herein, the designations [LVI] and [L / V / I] mean that the amino acid at that position can be leucine (Leu, L), valine (Val, V), or isoleucine (Ile, I), and so on. Unless further limited otherwise, amino acid X is defined such that it can be any of the 20 naturally occurring amino acids. DETAILED DESCRIPTION

[0082] The present invention provides novel dispersed proteins, preferably obtained from the genus Terribacillus (particularly Terribacillus saccharophilus). The dispersed proteins of the present invention preferably comprise at least 60% sequence identity with the polypeptide having SEQ ID NO: 1 and comprise an alteration at at least one amino acid position compared to the dispersed protein having SEQ ID NO: 1. In some aspects, the dispersed protein variants of the present invention comprise an amino acid sequence comprising at least two amino acid substitutions made at positions equivalent to those in SEQ ID NO: 1. The present invention also relates to methods for producing dispersed protein variants. The dispersed protein variants of the present invention have at least one improved property compared to the parent dispersed protein or compared to SEQ ID NO: 1. Properties include, but are not limited to, stability such as; stability in detergents, storage stability, stability in washing and thermal stability, wash performance, particularly deep cleaning performance, increased expression levels and reduced malodor. Preferably, the improved property is improved stability, such as stability in detergents (e.g., cleaning compositions). When household care surfaces (such as textiles) are soiled, the stains are typically a complex mixture of various organic substances (such as skin debris, such as dead cells, sweat and sebum, contamination from the outside and from, for example, the body). This biofouling is composed of different organic stains (such as proteins, starches, greases, and, for example, polysaccharides). Complex stains are usually stubborn and difficult to remove. WO 2017 / 186943 (Novozymes A / S) describes the use of dispersoids in laundry processes and detergents, which discloses dispersoids from the genus Bacillus, such as those with SEQ ID NO 1. Dispersoids have been shown to be very effective in removing stains associated with PNAG (poly-N-acetylglucosamine) from textiles under laundry conditions. However, when the enzyme is formulated into a cleaning composition, such as a detergent, its activity may decrease over time, particularly in liquid detergents. Obviously, preferably, the enzyme is stable in the cleaning and detergent composition during storage (storage stability) and during the washing process (stability during washing). The former can be measured in accelerated stability studies, where dispersed protein variants are tested under stress (e.g., increasing heat) and non-stress conditions, and the residual activity (RA) or half-life (T 1 / 2) and compared to the value of the parent (or another reference). The residual activity ratio (RAR) or half-life improvement factor (HIF) of the reference or parent dispersin can be assigned a value of 1. Thus, a dispersin variant having a RAR or HIF greater than 1 has improved stability under the conditions of measurement compared to the parent or reference dispersin. In a preferred embodiment of the invention, the dispersin variant has improved stability compared to the parent dispersin and is therefore superior to state-of-the-art dispersins.

[0083] Disperse protein variants

[0084] Some aspects of the invention relate to variants of dispersin of SEQ ID NO: 1 or variants of dispersin having at least 60% identity thereto, and to methods for producing variants of dispersin of SEQ ID NO: 1 or variants of dispersin having at least 60% identity thereto.

[0085] In one aspect, the invention relates to a dispersin protein comprising an alteration at one or more positions selected from the list consisting of: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100 、103、104、105、106、107、110、111、113、114、116、117、118、119、120、122、123、124、125、126、127、128、131、135、138、139、140、142、145、147、148、149、150、151、152、163、164、167、168、170、171、173、1 74, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261 1, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g., compared to SEQ ID NO 1.

[0086] In one aspect, the invention relates to a disperse protein comprising an alteration at one or more positions selected from the list consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has disperse protein activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0087] In a preferred aspect, the invention relates to a dispersin protein comprising an alteration at one or more positions selected from the group consisting of: 1, 2, 3, 12, 15, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 190 314, 315, 319, 321, 322, and 323, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g., compared to SEQ ID NO: 1.

[0088] In a preferred aspect, the invention relates to a dispersin protein comprising an alteration at one or more positions selected from the list consisting of: 3, 15, 49, 59, 140, 163, 186, 207, 215, 218, 225, 227, 232, 235, 237, 252, 260, 267, 272, 276, 279, 281, 288, 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0089] In a preferred aspect, the invention relates to a dispersin protein comprising a substitution at one or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0090] In a preferred aspect, the invention relates to a dispersin protein comprising a substitution at two or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0091] In a preferred aspect, the invention relates to a dispersin protein comprising substitutions at three or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0092] In a preferred aspect, the invention relates to a dispersin protein comprising a substitution at four or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0093] In a preferred aspect, the invention relates to a dispersin protein comprising a substitution at five or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0094] In a preferred aspect, the invention relates to a disperse protein comprising substitutions at six or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has disperse protein activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0095] In a preferred aspect, the invention relates to a dispersin protein comprising substitutions at seven or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0096] In a preferred aspect, the invention relates to a disperse protein comprising a substitution at eight or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has disperse protein activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0097] In a preferred aspect, the invention relates to a dispersin protein comprising a substitution at nine or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0098] In a preferred aspect, the invention relates to a dispersin protein comprising a substitution at ten or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0099] In a preferred aspect, a dispersin protein comprising a substitution at eleven or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281, 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g., compared to SEQ ID NO 1.

[0100] In a preferred aspect, the invention relates to a dispersin protein comprising a substitution at twelve or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0101] In a preferred aspect, the invention relates to a disperse protein comprising a substitution at thirteen or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has disperse protein activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0102] In a preferred aspect, the invention relates to a disperse protein comprising substitutions at fourteen or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has disperse protein activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0103] In a preferred aspect, the invention relates to a disperse protein comprising a substitution at fifteen or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has disperse protein activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0104] In a preferred aspect, the invention relates to a disperse protein comprising a substitution at sixteen or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has disperse protein activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0105] In a preferred aspect, the invention relates to a dispersin protein comprising substitutions at seventeen or more positions selected from the list consisting of: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has dispersin activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0106] In a preferred aspect, the invention relates to a disperse protein comprising a substitution at all of the following positions: 3, 15, 49, 59, 163, 186, 225, 227, 232, 235, 252, 260, 272, 279, 281 , 308, 309 and 312, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein the variant has disperse protein activity and preferably wherein the variant has improved stability compared to the parent, e.g. compared to SEQ ID NO 1.

[0107] The term "comprising a substitution" in this context means comprising a substitution compared to the starting dispersin or parent. Thus, an amino acid (e.g. position 3) of dispersin is replaced by another amino acid.

[0108] In some aspects, the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence of the mature parent disperse protein (e.g., disperse protein shown in SEQ ID NO: 1).

[0109] In some preferred aspects, the dispersin variant comprises an alteration selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30R, Y30 T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59 D. N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71 R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80V, K8 0W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R , L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V1 06K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107 M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D 111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S11 7*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L, E119G,E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H、Q262W、Q262Y、M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H2 72D, H272I, H272M, H272P, H272V, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M , F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y2 81*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y281R, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V , T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T 303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308 S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein the variant has dispersin activity and wherein each position corresponds to a position in the polypeptide set forth in SEQ ID NO: 1 (numbering is based on SEQ ID NO: 1).

[0110] In some preferred aspects, the alteration is a substitution and the dispersin variant comprises one or more substitutions selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30D, Y30L, Y30M, Y30N, Y31 0R, Y30T, Y30V, G32L, G32M, G32R, N43H, N43L, N45D, N45L, N45V, A49W, A49Y , Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N 59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63 D. T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L 71W, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74 L, S77A, D79V, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R , K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V1 06N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107 W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D 111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117D, S117H, S117N, S11 7P, E118A, E118D, E118G, E118L, E119G, E119W, T120I, T120L, T120M, T120V,T120W、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261A、E261D、E261R、E261W、Q262F、Q262H、Q262W、Q262Y、M263K、M263L、M263Q、D264C、D264E、Y265F、N267S、N267T、W268C、W268E、W268M、W268R、Y270F、A271D、A271G, H272D, H272I, H272M, H272P, H272V, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F2 76N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D , S279E, S279G, S279N, D280C, D280E, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y281R, Y282E, Y282N, H2 83I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V , T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D 304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309 A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein the variant has dispersin activity and wherein each position corresponds to a position in the polypeptide set forth in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1).

[0111] One aspect relates to a dispersin variant comprising one or more substitutions selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30A , Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43H, N43L, N45D, N45L, N45V, A49W, A49Y, Y52M, G54L, G54M, G54N, S56T, S56W, S 57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N6 2C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67 L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72C, S72D, S72E, S72F, S72G , S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D 81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K103V, K104N, K1 04W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V10 6Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107 W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V , D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117D, S117H, S117N,S117P、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122H、D122R、Y123W、Y124C、Y124H、Y124I、Y124K、Y124L、Y124M、Y124N、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125H、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128A、V128C、V128D、V128L、V128T、D131V、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138N、D138Q、D138R、D138S、D138V、D138W、E139W、V140I、D142R、D142W、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150A、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、S163P、G164D、G164E、G164H、G164S、G164V、V167A、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181K、S181N、S181P、S181Q、S181T、S181V、S181W、E185A、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203Q、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207K、D207N、D207Q、D207R、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215K、Q215R、Q215M、Q215L、S217V、T218A、T218L、T218Q、T218R、T218V、S221N、G222D、E224A、E224P、S225G、N227A、N227Q、N227R、N227S、N227T、N227K、D230N、D230R、D230T、D230W、E232D、E232V、N233D、N233E、N233H、N233Q、N233R、N233W、W234R、G235A、G235W、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251A、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256D、Q256E、Q256R、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N2 60T、N260V、N260W、N260Y、E261A、E261D、E261R、E261W、Q262F、Q262H 、Q262W、Q262Y、M263K、M263L、M263Q、D264C、D264E、D264N、Y265F、N267S、N267T、W268C、W268E、W268M、W268R、Y270F、A271D、A271G、H272 D、H272I、H272M、H272P、H272V、H272W、N273W、K274R、K274A、K274H、F276A、F276C、F276K、F276N、F276G、F276L、F276M、F276P、F276S、F27 6V、F276W、I278A、I278K、I278N、I278Q、I278V、S279C、S279D、S279E、 S279G、S279N、D280C、D280E、Y281A、Y281C、Y281H、Y281K、Y281N、Y28 1P、Y281R、Y282E、Y282N、H283I、A284I、A284L、A284N、A284P、A284T 、A284V、T287N、S288P、S288D、S288K、S288N、V290I、K291L、K291R、K2 91V、T296C、E300A、E300D、H301C、H301N、H301R、T303A、T303C、T303 G、T303K、T303Q、T303R、T303W、D304C、D304M、L305M、L305N、S306C、K 308A、K308D、K308E、K308G、K308I、K308L、K308Q、K308S、K308T、K30 8V、K308Y、K309A、K309C、K309D、K309E、K309G、K309H、K309L、K309M、 K309N、K309Q、K309S、K309T、K309I、K312A、K312E、K312L、K312M、K312N、K312Q、K312S、K312W、E314I、E314L、E314V、L315I、L315V、R319A、Y321F and N323R, wherein these positions correspond to positions of SEQ ID NO: 1 (numbered according to SEQ ID NO: 1), wherein the variant has sequence identity to the polypeptide shown in SEQ ID NO: 1, and has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to SEQ ID NO 1, and wherein the variant has dispersin, preferably β-1,6N-acetylglucosaminidase activity.

[0112] One aspect relates to a dispersin variant comprising one or more substitutions selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30D, Y30L, Y30M, Y30N , Y30R, Y30T, Y30V, G32L, G32M, G32R, N43H, N43L, N45D, N45L, N45V, A49W, A49Y, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N 59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T6 3C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71 R, L71V, L71W, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T , S72Y, I74L, S77A, D79V, K80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V 106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107L, K107M, K1 07T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D11 1Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117 D. S117H, S117N, S117P, E118A, E118D, E118G, E118L, E119G, E119W, T120I,T120L、T120M、T120V、T120W、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261A、E261D、E261R、E261W、Q262F、Q262H、Q262W、Q262Y、M263K、M263L、M263Q、D264C、D264E、Y265F、N267S、N267T、W268C、W268E、W268M、W268R, Y270F, A271D, A271G, H272D, H272I, H272M, H272P, H272V, H272W, N273W, K274R, K274A, K274H, F27 6A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y28 1R, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T30 3R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein these positions correspond to SEQ ID NO: 1 (numbered according to SEQ ID NO: 1), wherein the variant has sequence identity to the polypeptide shown in SEQ ID NO: 1, and has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to SEQ ID NO 1, and wherein the variant has dispersin, preferably β-1,6N-acetylglucosaminidase activity.

[0113] In some preferred aspects, the alteration is a substitution and the dispersin variant comprises one or more substitutions selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y12 3W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G , D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D 171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D17 4N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S1 99L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207 C. D207G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N26 7S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K , F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A 284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L30 5M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L,K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A, and N323R, wherein the variant has dispersin activity, preferably β-1,6N-acetylglucosaminidase activity and wherein each position corresponds to a position of the polypeptide set forth in SEQ ID NO: 1 (numbering is according to SEQ ID NO: 1).

[0114] In some aspects, the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence of the mature parent disperse protein (e.g., disperse protein shown in SEQ ID NO: 1).

[0115] In some aspects, the number of substitutions in the variants of the invention is 2-20, e.g., 2-10 and 2-5, such as 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 substitutions. In one aspect, the total number of substitutions compared to SEQ ID NO: 1 is 3-20, e.g., at least 5 to 20, at least 10 to 20 or such as at least 15 to 20 substitutions compared to a dispersin protein comprising the amino acid sequence shown in SEQ ID NO: 1.

[0116] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 2 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 2 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Leu, Ala, Trp, Arg or Asn. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of D2V, D2L, D2A, D2W, D2R and D2N, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0117] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 3 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 3 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe, Leu, Val, Tyr, Met, Ile, Pro or Thr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of Q3F, Q3L, Q3V, Q3Y, Q3M, Q3I, Q3P and Q3T, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0118] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 12 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 12 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala. In another aspect, the dispersin variant comprises or consists of the substitution S12A, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0119] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 15 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 15 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Tyr or Phe. In another aspect, the dispersin variant comprises or consists of the substitution H15Y or H15F, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0120] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 17 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 17 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Val, Arg, Met, Phe, Cys or Glu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of T17W, T17V, T17R, T17M, T17F, T17C and T17E, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0121] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 18 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 18 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu. In another aspect, the dispersin variant comprises or consists of the substitution V18L, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0122] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 19 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 19 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro, Asn, Lys or Asp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of E19P, E19N, E19K and E19D, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0123] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 22 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 22 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Val or Met. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K22A, K22V and K22M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0124] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 23 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 23 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Cys, Glu, Ile, Leu, Arg, Thr or Val. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S23A, S23C, S23E, S23I, S23L, S23R, S23T and S23V, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0125] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 24 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 24 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution L24I, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0126] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 25 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 25 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg. In another aspect, the dispersin variant comprises or consists of the substitution V25R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0127] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 26 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 26 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met. In another aspect, the dispersin variant comprises or consists of the substitution D26M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0128] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 30 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 30 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 30 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Val, Asp, Thr, Asn, Met, Ala, or Leu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of Y30R, Y30V, Y30D, Y30T, Y30N, Y30M, Y30A and Y30L, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0129] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 32 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 32 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Met or Leu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of G32R, G32M and G32L, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0130] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 34 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 34 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp. In another aspect, the dispersin variant comprises or consists of the substitution N34D, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0131] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 43 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 43 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 43 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu or His. In another aspect, the disperse protein variant comprises or consists of the substitution N43L or N43H, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0132] In another aspect, the dispersin variant comprises or consists of an amino acid deletion at the position corresponding to position 44 of SEQ ID NO: 1.

[0133] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 45 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 45 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Leu or Val. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of N45D, N45L and N45V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0134] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 49 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 49 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp or Tyr. In another aspect, the dispersin variant comprises or consists of the substitution A49W, A49Y, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0135] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 52 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 52 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 52 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met. In another aspect, the disperse protein variant comprises or consists of the substitution Y52M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0136] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 54 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 54 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Leu, or Met. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of G54N, G54L and G54M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0137] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 56 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 56 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp or Thr. In another aspect, the dispersin variant comprises or consists of the substitution S56W or S56T, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0138] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 57 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 57 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp. In another aspect, the dispersin variant comprises or consists of the substitution S57W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0139] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 58 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 58 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution E58N, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0140] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 59 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 59 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Cys, Asp, Glu, Phe, Met, Arg, Val or Trp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0141] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 60 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 60 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution T60V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0142] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 62 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 62 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Gln, His, Cys, or Asp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of N62W, N62Q, N62H, N62C and N62D, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0143] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 63 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 63 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Leu, Asn, Arg, Asp or Cys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of T63V, T63L, T63N, T63R, T63D and T63C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0144] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 67 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 67 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu or Ala. In another aspect, the dispersin variant comprises or consists of the substitution K67L or K67A, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0145] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 68 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 68 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu or Gln. In another aspect, the dispersin variant comprises or consists of the substitution N68L or N68Q, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0146] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 71 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 71 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, His, Asn, Trp, or Val. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of L71R, L71H, L71N, L71W and L71V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0147] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 72 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 72 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 72 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr, Gly, Cys, Met, Phe, Asn, Tyr, Arg, Ile, Asp or Glu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S72T, S72G, S72C, S72M, S72F, S72N, S72Y, S72R, S72I, S72D and S72E, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0148] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 74 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 74 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu. In another aspect, the dispersin variant comprises or consists of the substitution I74L, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0149] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 77 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 77 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala. In another aspect, the dispersin variant comprises or consists of the substitution S77A, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0150] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 79 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 79 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution D79V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0151] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 80 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 80 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 80 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Arg, Trp, Leu, Val, Gln, or His. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K80N, K80R, K80W, K80E, K80L, K80V, K80Q and K80H, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0152] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 81 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 81 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly, Ala, Trp, Leu, Trp, Thr, Val, Arg, Ser, or Asn. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of D81G, D81A, D81L, D81W, D81T, D81V, D81R, D81S and D81N, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0153] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 82 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 82 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution I82V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0154] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 90 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 90 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe. In another aspect, the dispersin variant comprises or consists of the substitution L90F, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0155] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 99 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 99 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln or Arg. In another aspect, the dispersin variant comprises or consists of the substitution E99Q or E99R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0156] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 100 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 100 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ser. In another aspect, the dispersin variant comprises or consists of the substitution L100S, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0157] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 103 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 103 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Val or Ala. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K103R, K103V and K103A, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0158] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 104 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 104 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn or Trp. In another aspect, the dispersin variant comprises or consists of the substitution K104N or K104W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0159] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 105 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 105 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution D105N, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0160] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 106 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 106 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Tyr, Leu, His, Glu, Trp, Asn, Arg, Met, Asp, Lys, Ala, or Gln. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of V106Y, V106L, V106H, V106E, V106W, V106N, V106R, V106M, V106D, V106K, V106A and V106Q, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0161] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 107 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 107 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met, Trp, Leu, Cys, Val, Ala, or Thr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K107M, K107W, K107L, K107C, K107V, K107A and K107T, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0162] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 110 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 110 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Val or Met. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of N110R, N110V and N110M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0163] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 111 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 111 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Ala, Arg, Asn, Trp, Met, Gln or Glu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of D111V, D111A, D111R, D111N, D111W, D111M, D111Q and D111E, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0164] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 113 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 113 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr. In another aspect, the dispersin variant comprises or consists of the substitution V113T, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0165] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 114 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 114 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ser or Cys. In another aspect, the dispersin variant comprises or consists of the substitution T114S or T114C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0166] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 116 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 116 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Asn or Arg. In another aspect, the disperse protein variant comprises or consists of the following substitutions: Y116D, Y116N or Y116R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0167] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 117 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 117 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 117 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, His, Asn, or Pro. In another aspect, the disperse protein variant comprises or consists of the following substitutions: S117D, S117H, S117N or S117P, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0168] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 118 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 118 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 118 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Asp, Gly or Leu. In another aspect, the disperse protein variant comprises or consists of the following substitutions: E118A, E118D, E118G or E118L, wherein the position corresponds to a position in SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0169] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 119 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 119 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly or Trp. In another aspect, the dispersin variant comprises or consists of the substitution E119G or E119W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0170] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 120 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 120 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile, Leu, Met, Val or Trp. In another aspect, the disperse protein variant comprises or consists of the following substitutions: T120I, T120L, T120M, T120V or T120W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0171] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 122 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 122 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 122 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg or His. In another aspect, the disperse protein variant comprises or consists of the substitution D122R or D122H, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0172] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 123 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 123 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp. In another aspect, the dispersin variant comprises or consists of the substitution Y123W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0173] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 124 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 124 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile, Met, His, Arg, Val, Gln, Thr, Lys, Leu, Asn, Trp or Cys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of Y124I, Y124M, Y124H, Y124R, Y124V, Y124Q, Y124T, Y124K, Y124L, Y124N, Y124W and Y124C, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0174] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 125 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 125 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Cys, Gly, His, Lys, or Gln. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: D125R, D125C, D125G, D125H, D125K and D125Q, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0175] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 126 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 126 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution N126V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0176] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 127 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 127 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Trp, Gln, Met, Asp, His or Lys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of R127L, R127W, R127Q, R127M, R127D, R127H and R127K, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0177] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 128 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 128 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Asp, Ala, Cys, or Thr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: V128L, V128D, V128A, V128C and V128T, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0178] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 131 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 131 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution D131V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0179] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 135 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 135 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 135 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met, Tyr, Ala, Asp, Glu, or Lys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of Q135M, Q135Y, Q135A, Q135D, Q135E and Q135K, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0180] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 138 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 138 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met, Val, Leu, Trp, Ser, Arg, Lys, Asn or Gln. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of D138M, D138V, D138L, D138W, D138S, D138R, D138K, D138N and D138Q, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0181] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 139 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 139 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp. In another aspect, the dispersin variant comprises or consists of the substitution E139W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0182] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 140 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 140 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution V140I, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0183] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 142 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 142 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp or Arg. In another aspect, the dispersin variant comprises or consists of the substitution D142W or D142R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0184] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 145 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 145 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 145 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Asn, His, or Leu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of Y145V, Y145N, Y145H and Y145L, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0185] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 147 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 147 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Asp, Ala, Val, Gly, Arg, Ser, Met, Thr, Cys or Phe. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of P147L, P147D, P147A, P147V, P147G, P147R, P147S, P147M, P147T, P147C and P147F, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0186] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 148 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 148 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Asp, Ala, or Val. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K148L, K148D, K148A and K148V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0187] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 149 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 149 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Met or Leu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: F149N, F149M and F149L, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0188] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 150 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 150 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Arg, Asp, Lys, Mat, His, Trp, Val, Gly, Leu or Ala. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of E150N, E150R, E150D, E150K, E150M, E150H, E150W, E150V, E150Y, E150L and E150A, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0189] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 151 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 151 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Pro, Trp, Asp, Leu, Ser, Asn or Cys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of G151A, G151P, G151W, G151D, G151L, G151S, G151N and G151C, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0190] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 152 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 152 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Leu or Arg. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K152D, K152L and K152R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0191] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 163 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 163 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro. In another aspect, the dispersin variant comprises or consists of the substitution S163P, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0192] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 164 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 164 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, His, Ser, Glu or Val. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of G164D, G164H, G164S, G164E and G164V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0193] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 167 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 167 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Ala, Trp, Pro, Asp, Leu, Arg or Glu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of V167Q, V167A, V167W, V167P, V167D, V167L, V167R and V167E, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0194] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 168 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 168 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution H168N, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0195] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 170 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 170 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Lys, Ser, His, Asn, Arg, Glu, Phe, Met, Ala, Trp, Tyr, Val, Gln or Pro. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of L170D, L170K, L170S, L170H, L170N, L170R, L170E, L170F, L170M, L170A, L170W, L170Y, L170V, L170Q and L170P, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0196] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 171 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 171 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Ala, Trp, Arg, Cys, Glu, Lys, Tyr, Met, Gln or Leu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of D171V, D171A, D171W, D171R, D171C, D171E, D171K, D171Y, D171M, D171Q and D171L, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0197] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 173 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 173 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Cys. In another aspect, the dispersin variant comprises or consists of the substitution I173C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0198] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 174 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 174 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, His, Met, Asn or Val. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: D174W, D174H, D174M, D174N and D174V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0199] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 175 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 175 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Tyr. In another aspect, the dispersin variant comprises or consists of the substitution F175Y, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0200] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 177 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 177 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met. In another aspect, the dispersin variant comprises or consists of the substitution N177M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0201] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 178 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 178 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 178 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Trp, Ala, or Lys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of Q178R, Q178W, Q178A and Q178K, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0202] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 179 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 179 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr. In another aspect, the dispersin variant comprises or consists of the substitution I179T, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0203] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 181 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 181 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr, Phe, Gln, Gly, Asn, Cys, Lys, Trp, Val, Asp, Thr or Pro. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S181T, S181F, S181Q, S181G, S181N, S181C, S181K, S181W, S181V, S181D, S181T and S181P, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0204] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 185 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 185 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Arg, Met, Val or Trp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of E185A, E185R, E185M, E185V and E185W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0205] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 186 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 186 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Glu, Asn, Gln, Leu, Trp, Val, Ile, Lys, Met, Arg or His. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S186D, S186E, S186N, S186Q, S186L, S186W, S186V, S186I, S186K, S186M, S186R and S186H, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0206] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 187 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 187 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly, Arg, Trp, Val, Cys, Asp or Ser. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K187G, K187R, K187W, K187V, K187C, K187D and K187S, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0207] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 188 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 188 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro. In another aspect, the dispersin variant comprises or consists of the substitution Y188P, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0208] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 189 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 189 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Val or Trp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of E189V, E189L and E189W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0209] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 199 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 199 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Tyr, Met, Cys or Leu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S199Y, S199M, S199C and S199L, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0210] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 200 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 200 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Asp, Lys, Arg, Met, Phe, Asn or Leu. In another aspect, the dispersin variant comprises or consists of a substitution selected from the group consisting of E200W, E200D, E200K, E200R, E200M, E200F, E200N and E200L, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0211] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 203 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 203 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Thr, Pro, Cys, Leu, Ser, Met, Gln, Thr, Asp, Gly, Glu or Val. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of A203R, A203W, A203P, A203C, A203L, A203S, A203M, A203Q, A203T, A203D, A203G, A203E and A203V, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0212] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 204 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 204 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Tyr, Val, Trp or Met. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of N204L, N204Y, N204V, N204W and N204M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0213] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 205 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 205 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution L205I, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0214] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 207 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 207 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Lys, Ser, Cys, Gly, Asn, Val, Arg, Gln, Glu, Trp or Ala. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of D207S, D207C, D207G, D207N, D207V, D207R, D207Q, D207E, D207W, D207K and D207A, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0215] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 208 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 208 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Val, Cys, Trp, Asp, Thr, Gln, Gly or Leu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S208A, S208V, S208C, S208W, S208D, S208T, S208Q, S208G and S208L, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0216] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 210 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 210 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr. In another aspect, the dispersin variant comprises or consists of the substitution S210T, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0217] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 215 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 215 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 215 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Lys, Arg, Leu, or Met. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of Q215K, Q215R, Q215L and Q215M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0218] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 217 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 217 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val. In another aspect, the dispersin variant comprises or consists of the substitution S217V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0219] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 218 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 218 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Arg, Ala, Val or Leu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of T218Q, T218R, T218A, T218V and T218L, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0220] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 221 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 221 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution S221N, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0221] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 222 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 222 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp. In another aspect, the dispersin variant comprises or consists of the substitution G222D, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0222] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 224 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 224 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala or Pro. In another aspect, the dispersin variant comprises or consists of the substitution E224A or E224P, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0223] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 225 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 225 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly. In another aspect, the dispersin variant comprises or consists of the substitution S225G, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0224] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 227 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 227 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Gln, Arg, Ser, Thr or Lys. In another aspect, the disperse protein variant comprises or consists of the following substitutions: N227A, N227Q, N227R, N227S, N227T or N227K, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0225] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 230 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 230 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 230 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Arg, Trp, or Thr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: D230N, D230R, D230W and D230T, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0226] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 232 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 232 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp or Val. In another aspect, the dispersin variant comprises or consists of the substitution E232D or E232V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0227] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 233 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 233 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Asp, His, Gln, Arg or Trp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of N233E, N233D, N233H, N233Q, N233R and N233W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0228] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 234 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 234 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg. In another aspect, the dispersin variant comprises or consists of the substitution W234R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0229] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 235 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 235 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Ala, Ser, Val, Leu, Met, Asn, His, Glu, Phe, Pro or Ile. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of G235W, G235A, G235S, G235V, G235L, G235M, G235N, G235H, G235E, G235F, G235P and G235I, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0230] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 237 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 237 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp, Tyr, Gly, Met, Asn or Cys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S237W, S237Y, S237G, S237M, S237N and S237C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0231] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 244 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 244 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Met or Cys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: Y244E, Y244M and Y244C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0232] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 249 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 249 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Arg, Trp, His, Lys, or Tyr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of L249Q, L249R, L249W, L249H, L249K and L249Y, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0233] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 251 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 251 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Leu, Arg, Trp or Asn. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S251A, S251L, S251R, S251W and S251N, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0234] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 252 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 252 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro or Cys. In another aspect, the dispersin variant comprises or consists of the substitution N252P or N252C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0235] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 253 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 253 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp or Trp. In another aspect, the dispersin variant comprises or consists of the substitution G253D or G253W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0236] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 254 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 254 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile, Leu, Met or Tyr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: F254I, F254L, F254M and F254Y, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0237] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 256 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 256 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Arg or Asp. In another aspect, the disperse protein variant comprises or consists of the following substitutions: Q256E, Q256R or Q256D, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0238] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 260 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 260 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 260 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Leu, Cys, Arg, Met, Lys, Ala, Val, Ile, Thr, Glu, Trp, or Tyr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of N260Q, N260L, N260C, N260R, N260M, N260K, N260A, N260V, N260I, N260T, N260E, N260W and N260Y, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0239] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 261 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 261 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Asp, Arg, or Trp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: E261A, E261D, E261R and E261W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0240] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 262 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 262 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 262 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with His, Phe, Trp, or Tyr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: Q262H, Q262F, Q262W and Q262Y, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0241] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 263 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 263 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Leu, or Lys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: M263Q, M263L and M263K, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0242] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 264 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 264 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 264 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Cys, or Asn. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: D264E, D264C and D264N, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0243] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 265 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 265 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe. In another aspect, the dispersin variant comprises or consists of the substitution Y265F, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0244] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 267 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 267 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Thr or Ser. In another aspect, the dispersin variant comprises or consists of the substitution N267T or N267S, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0245] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 268 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 268 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Arg, Cys or Met. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of W268E, W268R, W268C and W268M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0246] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 270 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 270 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe. In another aspect, the dispersin variant comprises or consists of the substitution Y270F, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0247] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 271 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 271 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gly or Asp. In another aspect, the dispersin variant comprises or consists of the substitution A271G or A271D, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0248] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 272 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 272 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Met, Pro, Ile, Val or Trp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: H272D, H272M, H272P, H272I, H272V and H272W wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0249] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 273 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 273 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Trp. In another aspect, the dispersin variant comprises or consists of the substitution N273W, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0250] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 274 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 274 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with His, Arg or Ala. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K274H, K274R and K274A, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0251] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 276 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 276 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala, Asn, Lys, Cys, Gly, Leu, Met, Pro, Ser, Val or Trp. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of F276A, F276N, F276K, F276C, F276G, F276L, F276M, F276P, F276S, F276V and F276W, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0252] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 278 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 278 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Val, Gln, Ala, Asn, or Lys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: I278V, I278Q, I278A, I278N and I278K, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0253] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 279 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 279 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Cys, Glu, Asn, Asp or Gly. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S279C, S279E, S279N, S279D and S279G, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0254] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 280 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 280 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu or Cys. In another aspect, the dispersin variant comprises or consists of the substitution D280E or D280C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0255] In another aspect, the dispersin variant comprises or consists of a deletion of an amino acid at the position corresponding to position 281 of SEQ ID NO: 1. In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 281 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 281 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with His, Ala, Lys, Asn, Cys, Pro, or Arg. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of Y281H, Y281A, Y281K, Y281N, Y281C, Y281P and Y281R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0256] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 282 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 282 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn or Glu. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: Y282N and Y282E, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0257] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 283 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 283 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution H283I, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0258] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 284 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 284 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Ile, Val, Asn, Pro, or Thr. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of A284L, A284I, A284V, A284N, A284P and A284T, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0259] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 287 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 287 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn. In another aspect, the dispersin variant comprises or consists of the substitution T287N, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0260] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 288 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 288 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Pro, Asp, Asn, or Lys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of S288P, S288D, S288N and S288K, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0261] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 290 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 290 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile. In another aspect, the dispersin variant comprises or consists of the substitution V290I, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0262] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 291 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 291 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg, Leu or Val. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K291R, K291L and K291V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0263] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 296 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 296 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Cys. In another aspect, the dispersin variant comprises or consists of the substitution T296C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0264] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 300 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 300 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp or Ala. In another aspect, the dispersin variant comprises or consists of the substitution E300D or E300A, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0265] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 301 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 301 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asn, Cys, or Arg. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: H301N, H301C and H301R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0266] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 303 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 303 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Gln, Ala, Cys, Lys, Gly, Trp or Arg. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of T303Q, T303A, T303C, T303K, T303G, T303W and T303R, wherein the position corresponds to a position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0267] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 304 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 304 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met or Cys. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of: D304M and D304C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO 1.

[0268] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 305 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 305 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Met or Asn. In another aspect, the dispersin variant comprises or consists of L305M or L305N, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0269] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 306 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 306 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Cys. In another aspect, the dispersin variant comprises or consists of S306C, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0270] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 308 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 308 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Asp, Glu, Ala, Val, Gln, Ser, Tyr, Gly, Leu, Thr or Ile. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K308D, K308E, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T and K308I, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0271] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 309 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 309 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Gly, Cys, Leu, Asp, Gln, Asn, Thr, Ala, Ser, Met, His, or Ile. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K309E, K309G, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H and K309I, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0272] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 312 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 312 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Glu, Ala, Gln, Ser, Trp, Leu, Asn or Met. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of K312E, K312A, K312Q, K312S, K312W, K312L, K312N and K312M, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0273] In another aspect, the dispersin variant comprises, or consists of, a substitution at the position corresponding to position 314 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 314 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Leu, Val or Ile. In another aspect, the disperse protein variant comprises or consists of a substitution selected from the group consisting of E314L, E314V and E314I, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0274] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 315 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 315 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ile or Val. In another aspect, the dispersin variant comprises or consists of L315I or L315V, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0275] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 319 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 319 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Ala. In another aspect, the dispersin variant comprises or consists of R319A, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0276] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 321 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 321 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Phe. In another aspect, the dispersin variant comprises or consists of Y321F, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0277] In another aspect, the dispersin variant comprises or consists of a substitution at the position corresponding to position 323 of SEQ ID NO: 1. In another aspect, the amino acid at the position corresponding to position 323 of SEQ ID NO: 1 is substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val, preferably with Arg. In another aspect, the dispersin variant comprises or consists of N323R, wherein the position corresponds to the position of SEQ ID NO: 1 and wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 1.

[0278] In one aspect, the variant comprises or consists of one or more substitutions at a position selected from the group consisting of: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103 , 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174 , 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, and 323, wherein the position corresponds to SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1, which has dispersin, preferably β-1,6N-acetylglucosaminidase activity, and further, the variant has at least one improved property compared to a reference enzyme, for example compared to SEQ ID NO 1.

[0279] In one aspect, the variant comprises or consists of one or more substitutions at a position selected from the group consisting of: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205 04, 205, 207, 210, 215, 218, 221, 225, 227, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322, and 323, wherein the position corresponds to SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1, which has dispersin, preferably β-1,6N-acetylglucosaminidase activity, and further, the variant has at least one improved property compared to a reference enzyme, for example compared to SEQ ID NO 1.

[0280] In one aspect, the variant comprises or consists of one or more substitutions at positions selected from the group consisting of 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein the position corresponds to a position of SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1, it has dispersin, preferably β-1,6N-acetylglucosaminidase activity, and further, the variant has at least one improved property compared to a reference enzyme, e.g., compared to SEQ ID NO 1.

[0281] In one aspect, the variant comprises or consists of one or more of the alterations selected from the group consisting of D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30A, Y30B, Y31C, Y32E, 0D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H , N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S 56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59 W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K 67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72 E. S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D8 1R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K1 03V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106 M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K 107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D11 1R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D,S117H、S117N、S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124H、Y124I、Y124K、Y124L、Y124M、Y124N、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125H、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128A、V128C、V128D、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138N、D138Q、D138R、D138S、D138V、D138W、E139W、V140I、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150A、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、S163P、G164D、G164E、G164H、G164S、G164V、V167A、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181K、S181N、S181P、S181Q、S181T、S181V、S181W、E185A、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203Q、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207K、D207N、D207Q、D207R、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215K、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、S221N、G222D、E224A、E224P、S225G、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230N、D230R、D230T、D230W、E232D、E232V、N233D、N233E、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251A、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256D、Q256E、Q256R、N260*、N 260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260T、N26 0V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H 、Q262W、Q262Y、M263K、M263L、M263Q、D264*、D264C、D264E、D264N、Y265F、N 267S、N267T、W268C、W268E、W268M、W268R、Y270F、A271D、A271G、H272D、H272I、H272M、H272P、H272V、H272W、N273W、K274R、K274A、K274H、F276A、F276C 、F276K、F276N、F276G、F276L、F276M、F276P、F276S、F276V、F276W、I278A、I278K、I278N、I278Q、I278V、S279C、S279D、S279E、S279G、S279N、D280C、D28 0E、Y281*、Y281A、Y281C、Y281H、Y281K、Y281N、Y281P、Y281R、Y282E、Y282 N、H283I、A284I、A284L、A284N、A284P、A284T、A284V、T287N、S288P、S288D、 S288K、S288N、V290I、K291L、K291R、K291V、T296C、E300A、E300D、H301C、H3 01N、H301R、T303A、T303C、T303G、T303K、T303Q、T303R、T303W、D304C、D304 M、L305M、L305N、S306C、K308A、K308D、K308E、K308G、K308I、K308L、K308Q、 K308S、K308T、K308V、K308Y、K309A、K309C、K309D、K309E、K309G、K309H、K3 09L、K309M、K309N、K309Q、K309S、K309T、K309I、K312A、K312E、K312L、K312M、K312N、K312Q、K312S、K312W、E314I、E314L、E314V、L315I、L315V、R319A、Y321F and N323R, wherein the position corresponds to the position of SEQ ID NO: 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity to the polypeptide shown in SEQ ID NO: 1, it has dispersin, preferably β-1,6N-acetylglucosaminidase activity, and further, the variant has at least one improved property compared to a reference enzyme, for example compared to SEQ ID NO 1.

[0282] In one aspect, the variant comprises or consists of one or more of the substitutions selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y1 24I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125 K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F1 75Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185M , E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A 203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D20 7G, S210T, Q215R, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M, Y244C , N252P, N252C, Q256E, Q262H, N260Q, M263Q, D264E, Y265F, N267T, N267S, Y2 70F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276K, F276 C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A284T, A 284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L305M, L30 5N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L, K308T,K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A, and N323R, wherein the position corresponds to the position of SEQ ID NO: 1, wherein the variant is identical to SEQ ID NO: 2. The polypeptide shown in NO: 1 has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% identity, which has disperse protein, preferably β-1,6N-acetylglucosaminidase activity, and in addition, compared to a reference enzyme, for example compared to SEQ ID NO 1 or compared to SEQ ID NO 15, the variant has at least one improved property.

[0283] In some respects, the improved characteristic is increased stability, such as improved detergent stability, improved wash stability and improved thermostability. Some aspects of the present invention relate to the dispersin variants having an improvement factor (e.g., measured with HIF) higher than 1 when the dispersin variants are tested for a characteristic of interest in a related assay, wherein the characteristic with reference to dispersin is given a value of 1. In some respects, the characteristic is stability, such as storage stability. Some aspects of the present invention relate to the dispersin variants having an improvement factor (e.g., measured with HIF) higher than 1 when the dispersin variants are tested for a characteristic of interest in a assay described in example 2, 3, 4, 5 or 6, wherein the characteristic with reference to dispersin is given a value of 1. In some respects, the characteristic is stability, such as storage stability.

[0284] In some aspects, the improved property is increased stability, such as improved detergent stability, improved wash stability, and improved thermal stability. Some aspects of the invention relate to dispersin variants having an improvement factor greater than 1 when the dispersin variant is tested for a property of interest in a relevant assay, wherein the property of the reference dispersin is assigned a value of 1. In some aspects, the property is stability, such as storage stability.

[0285] In some aspects, the improved property is improved detergent stability.

[0286] In some aspects, the improved property is improved protease stability.

[0287] In some respects,

[0288]

[0289] As shown in Examples 2a and 3, variants according to the invention are improved when the residual activity ratio is defined as being higher than 1 compared to a reference disperse protein under the conditions of measurement. In some aspects, the variants are improved compared to a reference disperse protein.

[0290] In some aspects, variants according to the invention have improved stability relative to a reference disperse protein as measured by a residual activity ratio (RAR) greater than 1.0.

[0291] The residual activity ratio (RAR) of the disperse protein variants of the present invention can be calculated as RA 变体 / RA 参考 As shown in Examples 2a and 3, improved variants were identified as variants having a residual activity ratio (RAR) greater than 1.0 compared to the reference disperse protein.

[0292] In some aspects, variants according to the invention have improved stability relative to a reference dispersin protein as measured by a Half-Life Improvement Factor (HIF) greater than 1.0.

[0293] The half-life improvement factor (HIF) of the dispersin variants of the present invention can be calculated as T 1 / 2变体 / T 1 / 2参考As shown in Example 2b, improved variants are identified as variants having a half-life improvement factor HIF greater than 1.0 compared to a reference disperse protein. Improved alterations, e.g., substitutions, according to the present invention are variants that result in an improvement factor, e.g., HIF or RAR, greater than 1 or that have at least one improved property compared to a starting molecule (i.e., a precursor, a reference parent polypeptide, etc.). In particularly preferred aspects, disperse protein variants comprising alterations according to the present invention produce disperse protein variants with improved stability, wherein HIF>1.0. In some aspects, compared to a reference disperse protein, e.g., SEQ ID NO: 1 or compared to SEQ ID NO: The variants according to the invention have a half-life improvement factor (HIF) of at least: 1.1; 1.2; 1.3; 1.4; 1.5; 1.6; 1.7; 1.8; 1.9; 2.0; 2.1; 2.2; 2.3; 2.4; 2.5; 2.6; 2.7, 2.8; 2.9; 3.0, 3.1; 3.2; 3.3; 3.4; 3.5, 3.6, 3.7, 3.8, 3.9; 4.0, 4.1; 4.2; 4.3; 4.4; 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1; 5.2; 5.3; 5.4; 5.5, 5.6, 5.7, 5.8, 5.9; 3.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1, 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 12, 15, 16, 20, 25, or 30.

[0294] In preferred aspects, the combination of mutations according to the present invention produces a disperse protein variant with improved stability, wherein RAR>1.0. In some aspects, compared to a reference disperse protein, such as SEQ ID NO: 1 or compared to SEQ ID NO: The variants according to the invention have a residual activity ratio (RAR) of at least: 1.1; 1.2; 1.3; 1.4; 1.5; 1.6; 1.7; 1.8; 1.9; 2.0; 2.1; 2.2; 2.3; 2.4; 2.5; 2.6; 2.7, 2.8; 2.9; 3.0, 3.1; 3.2; 3.3; 3.4; 3.5, 3.6, 3.7, 3.8, 3.9; 4.0, 4.1; 4.2; 4.3; 4.4; 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1; 5.2; 5.3; 5.4; 5.5, 5.6, 5.7, 5.8, 5.9; 3. .0, 6.1; 6.2; 6.3; 6.4; 6.5, 6.6, 6.7, 6.8, 6.9; 7.0, 7.1; 7.2; 7.3; 7.4; 7.5, 7.6, 7.7, 7.8, 7.9; 8.0, 8.1; 8.2; 8.3; 8.4; 8.5, 8.6, 8.7, 8.8, 8.9; 9.0, 9.1; 9.2; 9.3; 9.4; 9.5, 9.6, 9.7, 9.8, 9.9; 10.0, 10.1; 10.2; 10.3; 10.4; 10.5, 10.6, 10.7, 10.8, 10.9; 12, 15, 16, 20, 25 or 30.

[0295] A preferred embodiment relates to a dispersin variant with improved stability, wherein HIF>1.0 compared to SEQ ID NO: 1 or compared to SEQ ID NO 15. A preferred embodiment relates to a dispersin variant with improved stability, wherein the half-life improvement factor is at least 1.5 compared to SEQ ID NO: 1 or compared to SEQ ID NO 15 when measured as described in Example 2a.

[0296] A preferred embodiment relates to a dispersin variant with improved stability, wherein the RAR>1.0 compared to SEQ ID NO: 1 or compared to SEQ ID NO 15. A preferred embodiment relates to a dispersin variant with improved stability, wherein the residual activity ratio (RAR) is at least 1.5 compared to SEQ ID NO: 1 or compared to SEQ ID NO 15 when measured as described in Examples 2a and 3.

[0297] A preferred embodiment relates to a dispersin variant with improved stability, wherein HIF>1.0 compared to SEQ ID NO: 1. A preferred embodiment relates to a dispersin variant with improved stability, wherein the half-life improvement factor (HIF) is at least 1.2, such as 1.5, compared to SEQ ID NO: 1 or compared to SEQ ID NO: 15, wherein HIF is calculated as T 1 / 2分散蛋白变体 / T 1 / 2参考 and the half-life (T 1 / 2 , in minutes) is calculated as: 20 minutes x LN(0.5) / LN(RA) and the residual activity (RA) of each dispersin variant and reference dispersin (e.g., SEQ ID NO: 1 or 15) is calculated as: slope (stressed sample, 56°C for 60 min) / slope (unstressed sample, 21°C for 60 min), e.g., as described in Example 2b.

[0298] A preferred embodiment relates to a dispersin variant with improved stability, wherein the residual activity ratio (RAR) is > 1.0 compared to SEQ ID NO: 1 or compared to SEQ ID NO 15. A preferred embodiment relates to a dispersin variant with improved stability, wherein the residual activity ratio (RAR) is at least 1.2, such as 1.5, compared to SEQ ID NO: 1 or compared to SEQ ID NO 15, wherein the RAR is calculated as:

[0299]

[0300] As described in 2a and 3 in the Examples section.

[0301] A preferred aspect of the invention relates to a dispersin variant comprising an alteration, e.g., a substitution, compared to the dispersin protein having SEQ ID NO: 1 at a position selected from the group consisting of: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115 03, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174 4, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261 , 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, and 323, wherein these positions correspond to SEQ 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 or the polypeptide sequence shown in SEQ ID NO 15, wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity and wherein, compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15, the variant has improved stability as measured by an improved factor, e.g., RAR or HIF > 1.0.

[0302] A preferred aspect of the invention relates to a dispersin variant comprising an alteration, e.g., a substitution, compared to the dispersin protein having SEQ ID NO: 1 at a position selected from the group consisting of: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322, and 323, wherein these positions correspond to SEQ 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 or the polypeptide sequence shown in SEQ ID NO 15, wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity and wherein the variant has improved stability compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15 as measured by an improvement factor, e.g., RAR or HIF > 1.0.

[0303] A preferred aspect of the invention relates to a dispersin variant comprising an alteration, e.g., a substitution, at a position selected from the group consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, compared to the dispersin protein having SEQ ID NO: 1, wherein these positions correspond to positions of SEQ ID NO: 1 (numbered according to SEQ ID NO: 1), wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 or the polypeptide sequence shown in SEQ ID NO: 15, wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity and wherein the variant has the same sequence identity as SEQ ID NO: 1 The variant has improved stability as measured by an improved factor, eg, RAR or HIF > 1.0, compared to the polypeptide sequence shown in NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO: 15.

[0304] A preferred aspect of the invention relates to a dispersin variant comprising one or more alterations, e.g., substitutions, selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26 M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32 R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M , G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F , N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S 72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K 80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E9 9Q, E99R, L100S, K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106 D. V106E, V106H, V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y , K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V , D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C,T114S、Y116D、Y116N、Y116R、S117*、S117D、S117H、S117N、S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124H、Y124I、Y124K、Y124L、Y124M、Y124N、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125H、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128A、V128C、V128D、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138N、D138Q、D138R、D138S、D138V、D138W、E139W、V140I、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150A、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、S163P、G164D、G164E、G164H、G164S、G164V、V167A、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181K、S181N、S181P、S181Q、S181T、S181V、S181W、E185A、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203Q、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207K、D207N、D207Q、D207R、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215K、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、S221N、G222D、E224A、E224P、S225G、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230N、D230R、D230T、D230W、E232D、E232V、N233D、N233E、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251A、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F2 54M、F254Y、Q256D、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260 K、N260L、N260M、N260Q、N260R、N260T、N260V、N260W、N260Y、E261*、E261A、 E261D、E261R、E261W、Q262*、Q262F、Q262H、Q262W、Q262Y、M263K、M263L、M26 3Q、D264*、D264C、D264E、D264N、Y265F、N267S、N267T、W268C、W268E、W268M、W268R、Y270F、A271D、A271G、H272D、H272I、H272M、H272P、H272V、H272W、N 273W、K274R、K274A、K274H、F276A、F276C、F276K、F276N、F276G、F276L、F276M、F276P、F276S、F276V、F276W、I278A、I278K、I278N、I278Q、I278V、S279C、 S279D、S279E、S279G、S279N、D280C、D280E、Y281*、Y281A、Y281C、Y281H、Y281K、Y281N、Y281P、Y281R、Y282E、Y282N、H283I、A284I、A284L、A284N、A284 P、A284T、A284V、T287N、S288P、S288D、S288K、S288N、V290I、K291L、K291R、 K291V、T296C、E300A、E300D、H301C、H301N、H301R、T303A、T303C、T303G、T30 3K、T303Q、T303R、T303W、D304C、D304M、L305M、L305N、S306C、K308A、K308D、K308E、K308G、K308I、K308L、K308Q、K308S、K308T、K308V、K308Y、K309A、K 309C、K309D、K309E、K309G、K309H、K309L、K309M、K309N、K309Q、K309S、K309T、K309I、K312A、K312E、K312L、K312M、K312N、K312Q、K312S、K312W、E314I、E314L, E314V, L315I, L315V, R319A, Y321F and N323R, wherein these positions correspond to positions of SEQ ID NO: 1 (numbering according to SEQ ID NO: 1), wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100% sequence identity to the polypeptide set forth in SEQ ID NO: 1 or the polypeptide sequence set forth in SEQ ID NO: 15, wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity and wherein the variant has a β-1,6N-acetylglucosaminidase activity compared to the polypeptide sequence set forth in SEQ ID NO: 1 or to the polypeptide sequence set forth in SEQ ID NO: The variant has improved stability as measured by an improved factor, e.g., RAR or HIF > 1.0, compared to the polypeptide sequence shown in FIG15.

[0305] A preferred aspect of the invention relates to a dispersin variant comprising one or more alterations, e.g., substitutions, compared to the dispersin protein having SEQ ID NO: 1 selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30 L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44 *, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W , S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V , N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K 80E, K80H, K80L, K80N, K80Q, K80V, K80W, D81A, D81G, D81L, D81R, D81S, D 81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K104N, K10 4W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106M, V106N, V10 6Q, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K107V, K107W, N110 M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D111R, D111V, D111W , V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D, S117H, S117N,S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R, E261W, Q262*, Q262F, Q262H, Q262W, Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, H272M, H272P, H272V, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y281R, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K308L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein these positions correspond to SEQ 1, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 100%, at least 101%, at least 102%, at least 103%, at least 104%, at least 105%, at least 106%, at least 107%, at least 108%, at least 109%, at least 110%, at least 111%, at least 112%, at least 113%, at least 114%, at least 115%, at least 116%, at least 117%, at least 118%, at least 119%, at least 120%, at least 121%, at least 122%, at least 123%, at least 124%, at least 125%, at least 126%, at least 127%, at least 128%, at least 129%, at least 130%, at least 131%, at least 132%, at least 133%, at least 134%, at least 135%, at least 136%, at least 137%, at least 138%, at least 139%, at least 140%, at least 141%, at least 142%, at least 143%, at least 144%, at least 145%, at least 146%, at least 147%, at least 148%, at least 149%, at least 15At least 98% or at least 99% but less than 100% sequence identity, wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity and wherein the variant has improved stability as measured by an improved factor, e.g., RAR or HIF>1.0, compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO 15.

[0306] A preferred aspect of the invention relates to a dispersin variant comprising one or more alterations, e.g., substitutions, selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L 170S, L170H, D171E, D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D 174H, D174M, D174N, F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S1 81N, S181C, E185R, E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y1 88P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L20 5I, D207N, D207S, D207C, D207G, S210T, Q215R, T218Q, N227T, N227K, E23 2D, G235W, S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263 Q, D264E, Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273 W, K274H, F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E , D280C, Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R , T296C, T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V,8Q, K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A, and N323R, wherein these positions correspond to positions in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1), wherein the variant is identical to the polypeptide set forth in SEQ ID NO: 1. 15, wherein the variant has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% but less than 100% sequence identity, wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity and wherein the variant has improved stability compared to the polypeptide sequence shown in SEQ ID NO: 1 or compared to the polypeptide sequence shown in SEQ ID NO: 15, as measured by having an improved factor, e.g., RAR or HIF > 1.0.

[0307] In some preferred aspects, the disperse protein variants of the invention comprise an alteration at one or more positions selected from the group consisting of: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100 、103、104、105、106、107、110、111、113、114、116、117、118、119、120、122、123、124、125、126、127、128、131、135、138、139、140、142、145、147、148、149、150、151、152、163、164、167、168、170、171、173、 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 and 323, wherein the variant is the same as SEQ The polypeptide shown in SEQ ID NO: 1 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1) and wherein the variant has at least one improved property compared to the parent dispersin (e.g., a dispersin comprising a polypeptide having the amino acid sequence shown in SEQ ID NO: 1), preferably the improved property is improved stability, wherein stability is tested as shown in Examples 2a, 2b or 3.

[0308] In some preferred aspects, the disperse protein variants of the invention comprise an alteration at one or more positions selected from the group consisting of: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 190, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322, and 323, wherein the variant is identical to SEQ The polypeptide shown in SEQ ID NO: 1 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1) and wherein the variant has at least one improved property compared to the parent dispersin (e.g., a dispersin comprising a polypeptide having the amino acid sequence shown in SEQ ID NO: 1), preferably the improved property is improved stability, wherein stability is tested as shown in Examples 2a, 2b or 3.

[0309] In some preferred aspects, the disperse protein variants of the present invention comprise an alteration at one or more positions selected from the group consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1) and wherein the variant has at least one improved property compared to the parent disperse protein (e.g., a disperse protein comprising a polypeptide having the amino acid sequence shown in SEQ ID NO: 1), preferably the improved property is improved stability, wherein stability is tested as shown in Examples 2a, 2b or 3.

[0310] In some preferred aspects, the disperse protein variants of the invention comprise one or more alterations selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N4 3H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N5 9W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S7 2E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D8 1R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K1 03V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106 M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K 107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D11 1R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D,S117H、S117N、S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124H、Y124I、Y124K、Y124L、Y124M、Y124N、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125H、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128A、V128C、V128D、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138N、D138Q、D138R、D138S、D138V、D138W、E139W、V140I、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150A、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、S163P、G164D、G164E、G164H、G164S、G164V、V167A、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181K、S181N、S181P、S181Q、S181T、S181V、S181W、E185A、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203Q、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207K、D207N、D207Q、D207R、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215K、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、S221N、G222D、E224A、E224P、S225G、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230N、D230R、D230T、D230W、E232D、E232V、N233D、N233E、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251A、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256D、Q256E、Q256R、N260*、N 260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260T、N26 0V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H 、Q262W、Q262Y、M263K、M263L、M263Q、D264*、D264C、D264E、D264N、Y265F、N 267S、N267T、W268C、W268E、W268M、W268R、Y270F、A271D、A271G、H272D、H272I、H272M、H272P、H272V、H272W、N273W、K274R、K274A、K274H、F276A、F276C 、F276K、F276N、F276G、F276L、F276M、F276P、F276S、F276V、F276W、I278A、I278K、I278N、I278Q、I278V、S279C、S279D、S279E、S279G、S279N、D280C、D28 0E、Y281*、Y281A、Y281C、Y281H、Y281K、Y281N、Y281P、Y281R、Y282E、Y282 N、H283I、A284I、A284L、A284N、A284P、A284T、A284V、T287N、S288P、S288D、 S288K、S288N、V290I、K291L、K291R、K291V、T296C、E300A、E300D、H301C、H3 01N、H301R、T303A、T303C、T303G、T303K、T303Q、T303R、T303W、D304C、D304 M、L305M、L305N、S306C、K308A、K308D、K308E、K308G、K308I、K308L、K308Q、 K308S、K308T、K308V、K308Y、K309A、K309C、K309D、K309E、K309G、K309H、K3 09L、K309M、K309N、K309Q、K309S、K309T、K309I、K312A、K312E、K312L、K312M、K312N、K312Q、K312S、K312W、E314I、E314L、E314V、L315I、L315V、R319A、Y321F and N323R, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1) and wherein the variant has at least one improved property compared to the parent dispersin (e.g., a dispersin comprising a polypeptide having the amino acid sequence shown in SEQ ID NO: 1), preferably the improved property is improved stability, wherein stability is tested as shown in Examples 2a, 2b or 3.

[0311] In some preferred aspects, the disperse protein variants of the invention comprise one or more alterations selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I, Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D1 25K, D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E , D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N, F 175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R, E185 M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C, S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S, D207C, D20 7G, S210T, Q215R, T218K, T218Q, N227T, N227K, E232D, G235W, S237W, Y244M , Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E, Y265F, N267T, N2 67S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H, F276A, F276N, F276 K, F276C, I278V, S279N, S279D, S279G, D280E, D280C, Y281P, Y282N, H283I, A 284T, A284L, A284I, S288K, V290I, K291R, T296C, T303Q, D304M, D304C, L30 5M, L305N, S306C, K308D, K308A, K308V, K308Q, K308S, K308Y, K308G, K308L,98%, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A, and N323R, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide set forth in SEQ ID NO: 1, wherein each position corresponds to a position of the polypeptide set forth in SEQ ID NO: 1 (according to SEQ ID NO: 1). ID NO: 1), and wherein the variant has at least one improved property compared to the parent dispersin (e.g., a dispersin comprising a polypeptide having the amino acid sequence shown in SEQ ID NO: 1), preferably the improved property is improved stability, wherein stability is tested as shown in Example 2a, 2b or 3.

[0312] In some preferred aspects, the disperse protein variants of the invention comprise an alteration at one or more positions selected from the group consisting of: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 1 74, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261 1, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, and 323, wherein each position corresponds to SEQ 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, 2.00, 2.01, 2.02, 2.03, 2.04, 2.05, 2.06, 2.07, 2.08, 2.09, 2.10, 2.11, 2.12, 2.13, 2.14, 2.15, 2.20, 2.21, 2.22, 2.23, 2.24, 2.259. An improvement in half-life by a factor of at least 10, an increase in stability as measured by HIF or residual activity ratio, or RAR according to 2, 3, 4, 5, or 10, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to a position in the polypeptide shown in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1).

[0313] In some preferred aspects, the disperse protein variants of the invention comprise an alteration at one or more positions selected from the group consisting of: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205 , 207, 210, 215, 218, 221, 225, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322, and 323, wherein each substitution provides a dispersin variant, compared to a parent dispersin (e.g., comprising a protein having SEQ The dispersin variant has an increase in stability as measured by a half-life improvement factor, HIF or residual activity ratio, RAR of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (according to SEQ ID NO: 1). NO: 1 for numbering) and preferably wherein the variant has hexosaminidase, such as β-1,6N-acetylglucosaminidase activity.

[0314] In some preferred aspects, the dispersin variants of the invention comprise an alteration at one or more positions selected from the group consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein each substitution provides a dispersin variant having an increase in stability as measured by a half-life improvement factor, HIF or residual activity ratio, RAR of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5, or such as at least 10 compared to a parent dispersin (e.g., a dispersin comprising a polypeptide having the amino acid sequence set forth in SEQ ID NO: 1), wherein the variant is identical to the polypeptide of SEQ ID NO: 1. The polypeptide shown in NO:1 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO:1 (numbered according to SEQ ID NO:1) and preferably wherein the variant has hexosaminidase, such as β-1,6N-acetylglucosaminidase activity.

[0315] In some preferred aspects, the disperse protein variants of the present invention comprise one or more alterations (compared to SEQ ID 1 , wherein the changes are selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S , K103A, K103R, K103V, K104N, K104W, D105N, V106A, V106D, V106E, V106H , V106K, V106L, V106M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C , K107L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E , D111M, D111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D,Y116N、Y116R、S117*、S117D、S117H、S117N、S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124H、Y124I、Y124K、Y124L、Y124M、Y124N、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125H、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128A、V128C、V128D、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138N、D138Q、D138R、D138S、D138V、D138W、E139W、V140I、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150A、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、S163P、G164D、G164E、G164H、G164S、G164V、V167A、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181K、S181N、S181P、S181Q、S181T、S181V、S181W、E185A、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203Q、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207K、D207N、D207Q、D207R、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215K、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、S221N、G222D、E224A、E224P、S225G、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230N、D230R、D230T、D230W、E232D、E232V、N233D、N233E、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251A、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q2 56D、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260 M、N260Q、N260R、N260T、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、 E261W、Q262*、Q262F、Q262H、Q262W、Q262Y、M263K、M263L、M263Q、D264*、D26 4C、D264E、D264N、Y265F、N267S、N267T、W268C、W268E、W268M、W268R、Y270F 、A271D、A271G、H272D、H272I、H272M、H272P、H272V、H272W、N273W、K274R、K 274A、K274H、F276A、F276C、F276K、F276N、F276G、F276L、F276M、F276P、F276S、F276V、F276W、I278A、I278K、I278N、I278Q、I278V、S279C、S279D、S279E、 S279G、S279N、D280C、D280E、Y281*、Y281A、Y281C、Y281H、Y281K、Y281N、Y2 81P、Y281R、Y282E、Y282N、H283I、A284I、A284L、A284N、A284P、A284T、A284 V、T287N、S288P、S288D、S288K、S288N、V290I、K291L、K291R、K291V、T296C、 E300A、E300D、H301C、H301N、H301R、T303A、T303C、T303G、T303K、T303Q、T30 3R、T303W、D304C、D304M、L305M、L305N、S306C、K308A、K308D、K308E、K308G、K308I、K308L、K308Q、K308S、K308T、K308V、K308Y、K309A、K309C、K309D、K 309E、K309G、K309H、K309L、K309M、K309N、K309Q、K309S、K309T、K309I、K312A、K312E、K312L、K312M、K312N、K312Q、K312S、K312W、E314I、E314L、E314V、L315I, L315V, R319A, Y321F and N323R, wherein each substitution provides a dispersin variant having an increase in stability as measured by an improvement factor of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 in half-life, HIF or residual activity ratio, RAR, compared to a parent dispersin (e.g., a dispersin comprising a polypeptide having the amino acid sequence set forth in SEQ ID NO: 1), wherein the variant is identical to SEQ ID NO: 1. The polypeptide shown in SEQ ID NO: 1 has a sequence identity of at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100%, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1).

[0316] In some preferred aspects, the disperse protein variants of the present invention comprise one or more substitutions (same as SEQ ID 1, wherein the substitutions are selected from the group consisting of: D2V, Q3F, Q3I, S12A, H15Y, T17W, T17E, V18L, K22M, S23I, S23C, S23T, S23L, S23V, S23E, V25R, Y30L, A49W, A49Y, S56T, N59D, N59C, N59E, N59R, N59F, N59W, N59V, N62C, N62D, T63C, N68Q, S72D, S72E, I74L, S77A, I82V, L90F, E99Q, L100S, T114S, T114C, Y123W, Y124I , Y124M, Y124R, Y124V, Y124Q, Y124T, Y124K, D125R, D125C, D125G, D125K , D125Q, Q135M, D138R, D138K, D138Q, L170D, L170K, L170S, L170H, D171E , D171K, D171Y, D171M, D171Q, D171L, I173C, D174W, D174H, D174M, D174N , F175Y, Q178K, I179T, S181T, S181F, S181Q, S181G, S181N, S181C, E185R , E185M, E185V, S186K, S186M, S186R, S186H, K187G, Y188P, E189V, S199C , S199L, A203G, A203E, A203V, N204L, N204Y, N204V, L205I, D207N, D207S , D207C, D207G, S210T, Q215R, T218Q, S225G, N227T, N227K, E232D, G235W , S237W, Y244M, Y244C, N252P, N252C, Q256E, N260Q, Q262H, M263Q, D264E , Y265F, N267T, N267S, Y270F, H272M, H272P, H272I, H272V, N273W, K274H , F276A, F276N, F276K, F276C, I278V, S279N, S279D, S279G, D280E, D280C , Y281P, Y282N, H283I, A284T, A284L, A284I, S288K, V290I, K291R, T296C , T303Q, D304M, D304C, L305M, L305N, S306C, K308D, K308A, K308V, K308Q,K308S, K308Y, K308G, K308L, K308T, K308I, K309C, K309L, K309D, K309Q, K309N, K309T, K309A, K309S, K309M, K309H, K312A, K312Q, K312S, K312W, K312L, K312N, E314L, E314V, E314I, L315I, R319A, and N323R, wherein each substitution provides a dispersin variant that is different from a parent dispersin (e.g., comprising a protein having SEQ ID The dispersin variant has an increase in stability as measured by a half-life improvement factor, HIF or residual activity ratio, RAR of at least 1.05, such as 1.08, such as 1.1, such as 1.15, such as 1.2, such as 1.25, such as 1.3, such as 1.4, such as 1.5, such as 1.6, such as 1.7, such as 1.8, such as 1.9, such as 2, such as 3, such as 4, such as 5 or such as at least 10 compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1).

[0317] In some preferred embodiments of the present invention, the disperse protein variants of the present invention comprise one or more of the following substitutions: Q3F, Q3I, H15Y, T17W, A49W, N59E, V140I, S163P, S186R, D207N, Q215K, T218Q, S225G, N227T, E232D, G235W, S237W, N252P, N260Q, N267T, H272V, H272P, F276A, S279D, Y281P, S288P, K308Q, K308E, K309E, K312Q, K312E, wherein the variants are identical to those in SEQ ID NO: The polypeptide shown in NO:1 has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity, wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO:1 (numbered according to SEQ ID NO:1).

[0318] Variants of the present invention preferably comprise one or more of the following conserved motifs: GXDE (SEQ ID NO 8), [EQ] [NRSHA] [YVFL] [AGSTC] [IVLF] [EAQYN] [SN] (SEQ ID NO 9), [VIM] [LIV] G [GAV] DE [VI] [PSA] (SEQ ID NO 10), WND [SQR] [IVL] [TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD [DR] S (SEQ ID NO 14), which are shared among dispersed proteins of the Bacillus terrestris clade described below. As explained in the "Definitions", a clade comprises a group of polypeptides that are grouped together based on homology characteristics traced back to a common ancestor. Polypeptides that form a group of clades, such as shown in a phylogenetic tree, often share common properties and are more closely related than other polypeptides that are not in the clade.

[0319] A preferred aspect of the invention relates to variants of a disperse protein parent, wherein the variant comprises one or more of the following motifs: GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] (SEQ ID NO 10), WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14). 14), and wherein the variant comprises an alteration at one or more positions selected from the list consisting of: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 314, 315, 319, 321, and 323, wherein each position corresponds to a position in the polypeptide of SEQ ID NO: 1, wherein each alteration provides a dispersin variant having at least one improved property compared to the polypeptide set forth in SEQ ID NO: 1,wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity.

[0320] A preferred aspect of the invention relates to variants of a disperse protein parent, wherein the variant comprises one or more of the following motifs: GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14). 14), and wherein the variant comprises an alteration at one or more positions selected from the list consisting of: 2, 3, 12, 15, 17, 18, 22, 23, 24, 25, 30, 49, 56, 57, 59, 62, 63, 68, 72, 74, 77, 82, 90, 99, 100, 106, 114, 123, 124, 125, 135, 138, 140, 163, 167, 170, 171, 173, 174, 175, 178, 179, 181, 185, 186, 187, 188, 189, 199, 203, 204, 205, 207, 210, 215, 218, 221, 225, 227, 232, 235, 237, 244, 252, 256, 260, 262, 263, 264, 265, 267, 270, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 288, 290, 291, 296, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, 322, and 323, wherein each position corresponds to SEQ 1, wherein each alteration provides a disperse protein variant having at least one improved property compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbering according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity.

[0321] A preferred aspect of the invention relates to variants of a disperse protein parent, wherein the variant comprises one or more of the following motifs: GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), and wherein the variant comprises an alteration at one or more positions selected from the list consisting of: 31, 33, 36, 73, 75, 94, 141, 144, 241, 277, 294, 297, 299, wherein each position corresponds to a position of the polypeptide of SEQ ID NO: 1, wherein each alteration provides an alteration to the polypeptide of SEQ ID NO: 1. A disperse protein variant having at least one improved property compared to the polypeptide shown in SEQ ID NO: 1, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, such as at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO: 1 and wherein each position corresponds to a position of the polypeptide shown in SEQ ID NO: 1 (numbered according to SEQ ID NO: 1) and preferably wherein the variant has hexosaminidase, e.g., β-1,6N-acetylglucosaminidase activity.

[0322] A preferred aspect of the invention relates to variants of a disperse protein parent, wherein the variant comprises one or more of the following motifs: GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14), wherein the variant comprises one or more alterations (compared to SEQ ID NO 15). 1 ), wherein the changes are selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23D, 23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G3 2L, G32M, G32R, N34D, N43*, N43H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56 T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62Q, N62W , T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K 80R, K80V, K80W, D81A, D81G, D81L, D81N, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A,K103R、K103V、K104N、K104W、D105N、V106A、V106D、V106E、V106H、V106K、V106L、V106M、V106N、V106Q、V106R、V106W、V106Y、K107A、K107C、K107L、K107M、K107T、K107V、K107W、N110M、N110R、N110V、D111A、D111E、D111M、D111N、D111Q、D111R、D111V、D111W、V113T、T114C、T114S、Y116D、Y116N、Y116R、S117*、S117D、S117H、S117N、S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124H、Y124I、Y124K、Y124L、Y124M、Y124N、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125H、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128A、V128C、V128D、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138N、D138Q、D138R、D138S、D138V、D138W、E139W、V140I、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150A、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、S163P、G164D、G164E、G164H、G164S、G164V、V167A、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181K、S181N、S181P、S181Q、S181T、S181V、S181W、E185A、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203Q、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207K、D207N、D207Q、D207R、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S2...

Claims

1. A dispersin variant comprising an alteration, preferably a substitution, at one or more positions corresponding to one or more of the following positions: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110, 111, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125 14, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 178, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261, 262, 263, 264, 265, 267, 268, 270, 271, 272 , 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321 or 323, wherein the disperse protein variant has hexosaminidase activity, preferably β-1,6N-acetylglucosaminidase activity, and wherein the variant has increased stability, preferably thermal stability, measured as a half-life improvement factor (HIF) compared to the disperse protein having SEQ ID NO:

1.

2. A dispersin variant comprising at least one alteration selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19N, E19P, K22A, K22M, K22V, S23C, S23E, S23I, S23L, S23R, S23T, S23V, V25R, D26M, Y30*, Y30D, Y30L, Y30M, Y30N, Y30 30R, Y30T, Y30V, G32L, G32M, G32R, N43*, N43H, N43L, E44*, N45D, N45L, N45 V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N 59C, N59D, N59E, N59F, N59M, N59R, N59V, N59W, T60V, N62C, N62D, N62H, N62 Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L 71N, L71R, L71V, L71W, S72*, S72C, S72D, S72E, S72F, S72G, S72I, S72M, S72 N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K 80V, K80W, D81A, D81G, D81L, D81R, D81S, D81T, D81V, D81W, I82V, L90F, E99 Q, E99R, L100S, K103A, K103R, K104N, K104W, D105N, V106A, V106D, V106E, V1 06H, V106K, V106L, V106M, V106N, V106Q, V106W, V106Y, K107A, K107C, K107 L, K107M, K107T, K107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D 111N, D111Q, D111R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y11 6R, S117*, S117D, S117H, S117N, S117P, E118*, E118A, E118D, E118G, E118L,E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124I、Y124K、Y124L、Y124M、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128C、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138Q、D138R、D138S、D138V、D138W、E139W、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、G164D、G164E、G164H、G164S、G164V、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181N、S181P、S181Q、S181T、S181V、S181W、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207N、D207Q、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、G222D、E224A、E224P、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230R、D230T、D230W、E232D、E232V、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251L、S251N、S251R、S251W、N252P、N252C、G253D、G253W、F254I、F254L、F254M、F254Y、Q256E、Q256R、N260*、N260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H、Q262W、Q262Y, M263K, M263L, M263Q, D264*, D264C, D264E, Y265F, N267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A 271G, H272D, H272I, H272M, H272P, H272V, H272W, N273W, K274R, K274A, K274H, F276A, F276C, F276K, F276N, F276G, F27 6L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280 C, D280E, Y281*, Y281A, Y281C, Y281H, Y281K, Y281N, Y281P, Y281R, Y282E, Y282N, H283I, A284I, A284L, A284N, A284P, A284T, A284V, T287N, S288D, S288K, S288N, V290I, K291L, K291R, K291V, T296C, E300A, E300D, H301C, H301N, H301R, T 303A, T303C, T303G, T303K, T303Q, T303R, T303W, D304C, D304M, L305M, L305N, S306C, K308A, K308D, K308G, K308I, K30 8L, K308Q, K308S, K308T, K308V, K308Y, K309A, K309C, K309D, K309H, K309L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A, and N323R, wherein these positions correspond to positions in the polypeptide set forth in SEQ ID NO: 1, wherein the disperse protein variant has hexosaminidase activity, preferably β-1,6N-acetylglucosaminidase activity.

3. A disperse protein variant as described in any of the preceding claims, wherein the variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity with the polypeptide shown in SEQ ID NO:

1.

4. The disperse protein variant according to any one of the preceding claims, wherein the variant is obtained from the genus Terribacillus, preferably Terribacillus saccharophilus, Terribacillus goriensis or Terribacillus saccharophilus.

5. A disperse protein variant as claimed in any of the preceding claims, wherein the variant comprises one or more of the following motifs: GXDE (SEQ ID NO 8), [EQ][NRSHA][YVFL][AGSTC][IVLF][EAQYN][SN] (SEQ ID NO 9), [VIM][LIV]G[GAV]DE[VI][PSA] (SEQ ID NO 10), [WND[SQR][IVL][TLVM] (SEQ ID NO 11), QSTL (SEQ ID NO 12), NKFFY (SEQ ID NO 13) or NLD[DR]S (SEQ ID NO 14).

6. A dispersin variant as claimed in any one of the preceding claims, wherein the dispersin variant has improved stability as measured by an improved factor, e.g. RAR or HIF>1.0, compared to the dispersin having the polypeptide sequence shown in SEQ ID NO:

1.

7. A disperse protein variant as claimed in any one of the preceding claims, wherein the total number of substitutions compared to SEQ ID NO: 1 is 3-20, such as at least 5 to 20, at least 10 to 20 or such as at least 15 to 20 substitutions compared to a disperse protein comprising the amino acid sequence shown in SEQ ID NO:

1.

8. A method for obtaining a dispersed protein variant, the method comprising: a) introducing into a dispersin parent having hexosaminidase, preferably β-1,6-N-acetylglucosaminidase activity, an alteration, preferably a substitution, at one or more positions corresponding to: 2, 3, 12, 15, 17, 18, 19, 22, 23, 24, 25, 26, 30, 32, 34, 43, 44, 45, 49, 52, 54, 56, 57, 58, 59, 60, 62, 63, 67, 68, 71, 72, 74, 77, 79, 80, 81, 82, 90, 99, 100, 103, 104, 105, 106, 107, 110 of the polypeptide of SEQ ID NO: 1 , 111, 113, 114, 116, 117, 118, 119, 120, 122, 123, 124, 125, 126, 127, 128, 131, 135, 138, 139, 140, 142, 145, 147, 148, 149, 150, 151, 152, 163, 164, 167, 168, 170, 171, 173, 174, 175, 177, 1 78, 179, 181, 185, 186, 187, 188, 189, 199, 200, 203, 204, 205, 207, 208, 210, 215, 217, 218, 221, 222, 224, 225, 227, 230, 232, 233, 234, 235, 237, 244, 249, 251, 252, 253, 254, 256, 260, 261 1, 262, 263, 264, 265, 267, 268, 270, 271, 272, 273, 274, 276, 278, 279, 280, 281, 282, 283, 284, 287, 288, 290, 291, 296, 300, 301, 303, 304, 305, 306, 308, 309, 312, 314, 315, 319, 321, or 323, b) and recovering the variant.

9. The method of claim 8, comprising introducing one or more changes selected from the group consisting of: D2A, D2L, D2N, D2R, D2V, D2W, Q3F, Q3I, Q3L, Q3M, Q3P, Q3V, Q3Y, Q3T, S12A, H15F, H15Y, T17W, T17C, T17E, T17F, T17M, T17R, T17V, V18L, E19D, E19K, E19N, E19P, K22A, K22M, K22V, S23A, S23C, S23E, S23I, S23L, S23R, S23T, S23V, L24I, V25R, D26M, Y30*, Y30A, Y30D, Y30L, Y30M, Y30N, Y30R, Y30T, Y30V, G32L, G32M, G32R, N34D, N43*, N4 3H, N43L, E44*, N45D, N45L, N45V, A49W, A49Y, Y52*, Y52M, G54L, G54M, G54N, S56T, S56W, S57W, E58N, N59A, N59C, N59D, N59E, N59F, N59M, N59R, N59V, N5 9W, T60V, N62C, N62D, N62H, N62Q, N62W, T63C, T63D, T63L, T63N, T63R, T63V, K67A, K67L, N68L, N68Q, L71H, L71N, L71R, L71V, L71W, S72*, S72C, S72D, S7 2E, S72F, S72G, S72I, S72M, S72N, S72R, S72T, S72Y, I74L, S77A, D79V, K80*, K80E, K80H, K80L, K80N, K80Q, K80R, K80V, K80W, D81A, D81G, D81L, D81N, D8 1R, D81S, D81T, D81V, D81W, I82V, L90F, E99Q, E99R, L100S, K103A, K103R, K1 03V, K104N, K104W, D105N, V106A, V106D, V106E, V106H, V106K, V106L, V106 M, V106N, V106Q, V106R, V106W, V106Y, K107A, K107C, K107L, K107M, K107T, K 107V, K107W, N110M, N110R, N110V, D111A, D111E, D111M, D111N, D111Q, D11 1R, D111V, D111W, V113T, T114C, T114S, Y116D, Y116N, Y116R, S117*, S117D,S117H、S117N、S117P、E118*、E118A、E118D、E118G、E118L、E119G、E119W、T120I、T120L、T120M、T120V、T120W、D122*、D122H、D122R、Y123W、Y124C、Y124H、Y124I、Y124K、Y124L、Y124M、Y124N、Y124Q、Y124R、Y124T、Y124V、Y124W、D125C、D125G、D125H、D125K、D125Q、D125R、N126V、R127D、R127H、R127K、R127L、R127M、R127Q、R127W、V128A、V128C、V128D、V128L、V128T、D131V、Q135*、Q135A、Q135D、Q135E、Q135K、Q135M、Q135Y、D138K、D138L、D138M、D138N、D138Q、D138R、D138S、D138V、D138W、E139W、V140I、D142R、D142W、Y145*、Y145H、Y145L、Y145N、Y145V、P147A、P147C、P147D、P147F、P147G、P147L、P147M、P147R、P147S、P147T、P147V、K148A、K148D、K148L、K148V、F149L、F149M、F149N、E150A、E150D、E150H、E150K、E150L、E150M、E150N、E150R、E150V、E150W、E150Y、G151A、G151C、G151D、G151L、G151N、G151P、G151S、G151W、K152D、K152L、K152R、S163P、G164D、G164E、G164H、G164S、G164V、V167A、V167D、V167E、V167L、V167P、V167Q、V167R、V167W、H168N、L170A、L170D、L170E、L170F、L170H、L170K、L170M、L170N、L170P、L170Q、L170R、L170S、L170V、L170W、L170Y、D171A、D171C、D171E、D171K、D171L、D171M、D171Q、D171R、D171V、D171W、D171Y、I173C、D174H、D174M、D174N、D174V、D174W、F175Y、N177M、Q178*、Q178A、Q178K、Q178R、Q178W、I179T、S181C、S181D、S181F、S181G、S181K、S181N、S181P、S181Q、S181T、S181V、S181W、E185A、E185M、E185R、E185V、E185W、S186D、S186E、S186H、S186I、S186K、S186L、S186M、S186N、S186Q、S186R、S186V、S186W、K187C、K187D、K187G、K187R、K187S、K187V、K187W、Y188P、E189L、E189V、E189W、S199C、S199L、S199M、S199Y、E200D、E200F、E200K、E200L、E200M、E200N、E200R、E200W、A203C、A203D、A203E、A203G、A203L、A203M、A203P、A203Q、A203R、A203S、A203T、A203V、A203W、N204L、N204M、N204V、N204W、N204Y、L205I、D207A、D207C、D207E、D207G、D207K、D207N、D207Q、D207R、D207S、D207V、D207W、S208A、S208C、S208D、S208G、S208L、S208Q、S208T、S208V、S208W、S210T、Q215K、Q215R、Q215M、Q215L、Q215*、S217V、T218A、T218L、T218Q、T218R、T218V、S221N、G222D、E224A、E224P、S225G、N227A、N227Q、N227R、N227S、N227T、N227K、D230*、D230N、D230R、D230T、D230W、E232D、E232V、N233D、N233E、N233H、N233Q、N233R、N233W、W234R、G235W、G235A、G235E、G235F、G235H、G235I、G235L、G235M、G235N、G235P、G235S、G235V、S237C、S237G、S237M、S237N、S237W、S237Y、Y244C、Y244E、Y244M、L249H、L249K、L249Q、L249R、L249W、L249Y、S251A、S251L、S251N、S251R、S251W、N252P、N252C、<h2 style=";text-align:left;direction:ltr">G253D、G253W、F254I、F254L、F254M、F254Y、Q256D、Q256E、Q256R、N260*、N 260A、N260C、N260E、N260I、N260K、N260L、N260M、N260Q、N260R、N260T、N26 0V、N260W、N260Y、E261*、E261A、E261D、E261R、E261W、Q262*、Q262F、Q262H 、Q262W、Q262Y、M263K、M263L、M263Q、D264*、D264C、D264E、D264N、Y265F、N 267S, N267T, W268C, W268E, W268M, W268R, Y270F, A271D, A271G, H272D, H272I, H272M, H272P, H272V, H272W, N273W, K274R, K274A, K274H, F276A, F276C F276K, F276N, F276G, F276L, F276M, F276P, F276S, F276V, F276W, I278A, I278K, I278N, I278Q, I278V, S279C, S279D, S279E, S279G, S279N, D280C, D28 0E、Y281*、Y281A、Y281C、Y281H、Y281K、Y281N、Y281P、Y281R、Y282E、Y282 N、H283I、A284I、A284L、A284N、A284P、A284T、A284V、T287N、S288P、S288D、 S288K、S288N、V290I、K291L、K291R、K291V、T296C、E300A、E300D、H301C、H3 01N、H301R、T303A、T303C、T303G、T303K、T303Q、T303R、T303W、D304C、D304 M、L305M、L305N、S306C、K308A、K308D、K308E、K308G、K308I、K308L、K308Q、 K308S、K308T、K308V、K308Y、K309A、K309C、K309D、K309E、K309G、K309H、K3 09L, K309M, K309N, K309Q, K309S, K309T, K309I, K312A, K312E, K312L, K312M, K312N, K312Q, K312S, K312W, E314I, E314L, E314V, L315I, L315V, R319A,Y321F and N323R.

10. The method of any of the preceding claims, wherein the disperse protein variant has at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99%, but less than 100% sequence identity to the polypeptide shown in SEQ ID NO:

1.

11. A composition, preferably a cleaning composition, comprising a variant according to any one of claims 1 to 7.

12. A method of cleaning an item, the method comprising a) adding the dispersed protein variant of any one of claims 1 to 7 to the cleaning composition and b) washing the item with the composition, wherein the item is a textile or a hard surface.

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