Uniform mixing and cleaning structure for single pipe

Abstract

The invention provides a single-tube mixing and cleaning structure which comprises a mounting plate, a lifting mechanism is fixedly connected to the mounting plate, and an eccentric mechanism is mounted at the lifting end of the lifting mechanism; the rotating end of the eccentric mechanism is connected with the eccentric needle seat, the sampling needle is mounted on the eccentric needle seat and penetrates through a mounting hole of the sampling needle swab, and the sampling needle swab is hollow and is provided with a swab liquid inlet and a swab liquid outlet. According to the invention, the sampling needle can be effectively cleaned, and meanwhile, the functions of stirring and uniformly mixing samples by controllably and accurately positioning the position of the sampling needle can be realized, the stirring amplitude can be designed and controlled, the start-stop position of the sampling needle can be accurately controlled, the stirring speed of the sampling needle can be accurately controlled, and accurate positioning and uniform mixing of sample tubes with different diameters can be realized.

Description

Technical Field

[0001] The invention relates to the technical field of cytometers, in particular to a single-tube mixing and cleaning structure. Background Art

[0002] When using a flow cytometer, a sample containing cells or particles is suspended in a liquid and injected into the flow cytometer instrument, allowing the cells to pass through a focused laser beam one by one. The light refracted by the cells contains information about the characteristics of the cells and their components. Because these cells have been marked with fluorescent markers before measurement, these cells will absorb certain wavelengths of laser light and then re-emit fluorescence at a certain wavelength bandwidth. Flow cytometer experiments can detect tens of thousands of cells per second, and the data is collected by a computer for processing.

[0003] Flow cytometers include full-spectrum flow cytometers, and flow cytometers usually include sampling needles. The sampling needles of existing flow cytometers usually have a single function, which is only a sampling function, such as not having an automatic cleaning function. In the prior art, the sampling needles of flow cytometers are usually cleaned by a cleaning pool outside the needle, which has the disadvantage of inconvenient cleaning. Summary of the invention

[0004] The present invention provides a single-tube mixing and cleaning structure to solve the technical problems raised by the above-mentioned background technology.

[0005] In order to solve the above technical problems, the present invention discloses a single-tube mixing and cleaning structure, comprising: A mounting plate, to which a lifting mechanism is fixedly connected, and an eccentric mechanism is installed on the lifting end of the lifting mechanism; An eccentric needle seat, the rotating end of the eccentric mechanism is connected to the eccentric needle seat, the sampling needle is installed on the eccentric needle seat, the sampling needle passes through the mounting hole of the sampling needle swab, the sampling needle swab is hollow inside, and is provided with a swab liquid inlet and a swab liquid discharge port.

[0006] Preferably, the eccentric mechanism comprises: a motor mounting block, a motor mounting block is mounted on the lifting end of the lifting mechanism, a motor is mounted on the motor mounting block, an eccentric rotating shaft is fixed on the output shaft of the motor, and the eccentric rotating shaft is mounted on the eccentric needle seat through a first bearing; An optical coupler baffle is fixed on the eccentric rotating shaft, and a counting optical coupler is mounted on a motor mounting block. When the optical coupler baffle rotates, the counting optical coupler is passed through.

[0007] Preferably, it also includes: A first retaining shaft, wherein the first retaining shaft is fixed on the sampling needle swab, a fixing sleeve is fixedly arranged on the mounting plate, and the first retaining shaft is inserted into the fixing sleeve; Two guide retaining shafts are fixed on the sampling needle swab, and the two guide retaining shafts pass through the second bearing, and the second bearing is installed on the eccentric needle seat.

[0008] Preferably, the lifting mechanism includes: a fixed guide rail, the fixed guide rail is fixedly connected to the mounting plate, a slider is slidably connected to the fixed guide rail along the up and down directions, and a motor mounting block is installed on the slider; the slider is driven by the translation drive mechanism to slide on the fixed guide rail.

[0009] Preferably, it also includes: a tension spring fixing plate, the tension spring fixing plate is fixedly connected to the motor mounting block, a tension spring fixing pin is fixedly arranged on the eccentric needle seat, and the two ends of the guide-keeping tension spring are respectively connected to the tension spring fixing plate and the tension spring fixing pin.

[0010] Preferably, it also includes: The detection sleeve is detachably mounted on the outer side of the upper part of the sampling needle. A distance sensor is arranged on the outer peripheral side of the detection sleeve. The distance sensor is used to detect the vertical distance between the distance sensor and the side wall of the current sample tube. Each time sampling is performed, the sampling needle is inserted into the current sample tube to a preset depth, and then the distance sensor is controlled to perform several tests. A first calculation module, used for calculating an actual center state value based on a detection value of a ranging sensor; A temperature measurement module, used to detect the temperature of a temperature measurement point in the current sample tube before stirring the current sample tube; A second calculation module, used for calculating the actual temperature state value based on the temperature measurement module; A third calculation module, used for calculating a first target power of the stepper motor when stirring the current sample tube based on the first calculation module and the second calculation module; The first control module is used to control the actual power of the stepper motor to be the first target power when the sample tube is currently being stirred.

[0011] Preferably, the first calculation module calculates the actual center state value based on the following formula: ; is the actual center state value; is the actual detection value of the jth ranging sensor; N is the total number of ranging sensors; is the reference detection value of the distance measuring sensor; is the radius of the current sample tube; is the actual detection value of the j-1th distance measuring sensor; a temperature measurement module is used to detect the temperature of the temperature measurement point in the current sample tube before stirring the current sample tube; The second calculation module calculates the actual temperature state value based on the following formula; ; is the actual temperature state value; M is the total number of temperature measurement points; is the temperature detection value of the i-th temperature measurement point; is the reference temperature of the current sample in the current sample tube; for The total number of temperature measurement points corresponding to a value greater than 0; for The total number of temperature measurement points corresponding to less than 0; The third calculation module is based on the following calculation formula: ; P is the first target power of the stepper motor; is the theoretical power of the stepper motor when stirring the current type of sample in the sample tube with a radius of d; is the logarithm with base 10, and e is a natural constant.

[0012] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments.

[0013] Compared with the prior art, the present invention has the following beneficial effects: 1. It can realize the up and down linear motion of the sampling needle, the eccentric motion of the sampling needle, and the follow-up motion of the sampling needle swab; 2. The stirring intensity is controllable, which can achieve stirring and mixing with controllable intensity; 3. The stirring amplitude can be designed and controlled by changing the eccentric size; 4. The stop position of the sampling needle is controllable, and the exact stop position of the sampling needle can be controlled to achieve precise positioning; 5. The cleaning, stirring and up and down movement of the sampling needle are integrated into one design.

[0014] The present invention can realize the controllable stirring and mixing function of the sample while effectively cleaning the sampling needle and accurately positioning the sampling needle position. The stirring amplitude can be designed and controlled, the start and stop positions of the sampling needle can be accurately controlled, and the stirring speed of the sampling needle can be accurately controlled, so as to realize accurate positioning and mixing of sample tubes with different diameters. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings: Figure 1 It is a front view of the present invention; Figure 2 For the present invention Figure 1 Schematic diagram of the three-dimensional structure in the AA direction; Figure 3 The side view of the present invention Figure 1 ; Figure 4 The side view of the present invention Figure 2 ; Figure 5 For the present invention Figure 4 Middle BB view; Figure 6 It is a schematic diagram of an embodiment of an integrated circuit of the present invention.

[0016] In the figure: 1. Guide rail; 2. Slider; 3. Motor; 4. Eccentric shaft; 5. Eccentric needle seat; 6. Guide holding shaft; 7. Sampling needle; 8. Sampling needle swab; 9. Mounting plate; 11. Fixing sleeve; 12. Second bearing; 13. Guide holding spring; 14. Motor mounting block; 15. Tension spring fixing plate; 16. Optical coupling baffle; 17. Counting optical coupling; 18. Tension spring fixing pin; 19. Tension spring fixing screw; 20. Swab liquid inlet; 21. First bearing; 22. Second bearing fixing screw; 23. Swab liquid discharge port; 24. First holding shaft ; U1, first field effect transistor; U2, second field effect transistor; U3, third field effect transistor; U4, fourth field effect transistor; U5, fifth field effect transistor; U6, sixth field effect transistor; R1, first resistor; R2, second resistor; R3, third resistor; R4, fourth resistor; R5, fifth resistor; R6, sixth resistor; R7, seventh resistor; R8, eighth resistor; C1, first capacitor; C2, second capacitor; U7, power field effect transistor; V1, first power supply; V2, second power supply; D1, first diode; D2, second diode. DETAILED DESCRIPTION

[0017] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.

[0018] In addition, in the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes, and do not specifically refer to the order or sequence, nor are they used to limit the present invention. They are only used to distinguish components or operations described with the same technical terms, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions and technical features between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0019] The present invention provides the following embodiments Embodiment 1, the embodiment of the present invention provides a single tube mixing and cleaning structure, such as Figure 1-Figure 5 As shown, including: A mounting plate 9, to which a lifting mechanism is fixedly connected, and an eccentric mechanism is installed at the lifting end of the lifting mechanism; The eccentric needle seat 5, the rotating end of the eccentric mechanism is connected to the eccentric needle seat 5, the sampling needle 7 is installed on the eccentric needle seat 5, the sampling needle 7 passes through the installation hole of the sampling needle swab 8, the sampling needle swab 8 is hollow inside, and is provided with a swab liquid inlet 20 and a swab liquid discharge port 23. The sampling needle swab 8 is connected to the sampling needle 7, and the cleaning liquid can be input through the swab liquid inlet 20 to clean the sampling needle. The swab liquid inlet 20 can also be closed during sampling, and the swab liquid discharge port 23 can be opened to collect the liquid / sample in the sample tube through the sampling pump and output to the sample detection position.

[0020] Preferably, the eccentric mechanism comprises: a motor mounting block 14, a motor mounting block 14 is mounted on the lifting end of the lifting mechanism, a motor 3 is mounted on the motor mounting block 14, an eccentric rotating shaft 4 is fixed on the output shaft of the motor 3, and the eccentric rotating shaft 4 is mounted on the eccentric needle seat 5 through a first bearing 21; the eccentric rotating shaft 4 is a prior art, such as CN222026135U, CN206409506U; An optical coupling baffle 16 is fixed on the eccentric rotating shaft 4 , and a counting optical coupling 17 is mounted on the motor mounting block 14 . When the optical coupling baffle 16 rotates, it passes through the counting optical coupling 17 .

[0021] Preferably, it also includes: A first retaining shaft 24, wherein the first retaining shaft 24 is fixed on the sampling needle swab 8, a fixing sleeve 11 is fixedly arranged on the mounting plate 9, and the first retaining shaft 24 is inserted into the fixing sleeve 11; Two guide retaining shafts 6 are fixed on the sampling needle swab 8 , and the two guide retaining shafts 6 pass through the second bearing 12 , and the second bearing 12 is installed on the eccentric needle seat 5 .

[0022] Preferably, the lifting mechanism includes: a fixed guide rail 1, which is fixedly connected to the mounting plate 9, a slider 2 which is slidably connected to the fixed guide rail 1 along the up and down directions, and a motor mounting block 14 is installed on the slider 2; the slider 2 is driven by a translation drive mechanism (which can be an electric telescopic rod) to slide on the fixed guide rail 1, so as to control the depth of the sampling needle 7 inserted into the sample.

[0023] Preferably, it also includes: a tension spring fixing piece 15, the tension spring fixing piece 15 is fixedly connected to the motor mounting block 14, a tension spring fixing pin 18 is fixedly arranged on the eccentric needle seat 5, and both ends of the guide holding tension spring 13 are respectively connected to the tension spring fixing piece 15 and the tension spring fixing pin 18. The guide holding tension spring 13 is connected to the tension spring fixing piece 15 through a tension spring fixing screw 19.

[0024] The second bearing 12 is a linear bearing; wherein the second bearing 12 is connected to the eccentric needle seat 5 via a second bearing fixing screw 22 .

[0025] The working principle of the above technical solution is: The up and down movement of the slider 2 drives the motor 3 and the sampling needle 7 to move up and down, so that the sampling needle 7 can enter and exit the sample tube to take liquid when sampling; the rotation of the motor 3 drives the eccentric shaft 4 to make an eccentric movement, and the eccentric shaft 4 is inserted into the first bearing 21 and fixed on the eccentric needle seat 5, so that the rotation of the motor 3 drives the eccentric needle seat 5 to make an eccentric movement, and the eccentric needle seat 5 drives the sampling needle 7 to make an eccentric movement, and the guide holding spring 13 pulls the eccentric needle seat 5, so that the eccentric needle seat 5 only makes an eccentric movement without rotating itself, and the flexible connection between the guide holding spring 13 and the eccentric needle seat 5 can both resist the impact of rotation and hold the eccentric needle seat 5 Orientation, when the motor 3 stops rotating, the eccentric needle seat 5 is always in the same direction as the guide holding spring 13 and the direction is unique; the guide holding shaft 6 is fixed on the sampling needle swab 8, and passes through the second bearing 12 on the eccentric needle seat 5, so that the eccentric needle seat 5, the sampling needle swab 8 and the sampling needle 7 can maintain synchronous eccentric movement, and the second bearing 12 can make the guide holding shaft 6 slide smoothly; the sampling needle 7 passes through the sampling needle swab 8, and the sampling needle 7 can be effectively cleaned by the inlet and outlet flow on the swab; the first holding shaft 24 on the sampling needle swab 8 is inserted into the fixed sleeve 11, which can keep the sampling needle 7 swab from moving up and down.

[0026] The beneficial effects of the above technical solution are: 1. It can realize the up and down linear motion of the sampling needle 7, the eccentric motion of the sampling needle 7, and the follow-up motion of the sampling needle swab 8; 2. The stirring intensity is controllable, which can achieve stirring and mixing with controllable intensity; 3. The stirring amplitude can be designed and controlled by changing the eccentric size; 4. The stop position of the sampling needle 7 is controllable, and the exact stop position of the sampling needle 7 can be controlled to achieve precise positioning; 5. The cleaning of the sampling needle 7, the stirring of the sampling needle 7 and the up and down movement of the sampling needle are integrated into one design.

[0027] The present invention can realize the controllable stirring and mixing function of the sample while effectively cleaning the sampling needle 7 and accurately positioning the sampling needle 7. The stirring amplitude can be designed and controlled, the start and stop positions of the sampling needle can be accurately controlled, and the stirring speed of the sampling needle 7 can be accurately controlled, so as to realize accurate positioning and mixing of sample tubes with different diameters.

[0028] Embodiment 2, based on embodiment 1, further comprises: The detection sleeve is detachably mounted on the outer side of the upper part of the sampling needle 7. A distance sensor is arranged on the outer peripheral side of the detection sleeve. The distance sensor is used to detect the vertical distance between the distance sensor and the side wall of the current sample tube. Each time sampling is performed, the sampling needle 7 is inserted into the current sample tube to a preset depth, and then the distance sensor is controlled to perform several detections. A first calculation module, used for calculating an actual center state value based on a detection value of a ranging sensor; A temperature measurement module, used to detect the temperature of a temperature measurement point in the current sample tube before stirring the current sample tube; A second calculation module, used for calculating the actual temperature state value based on the temperature measurement module; A third calculation module, used for calculating a first target power of the stepper motor when stirring the current sample tube based on the first calculation module and the second calculation module; The first control module is used to control the actual power of the stepper motor to be the first target power when the sample tube is currently being stirred.

[0029] A first calculation module calculates the actual center state value based on the following formula; ; is the actual center state value; is the actual detection value of the jth ranging sensor; N is the total number of ranging sensors; is the reference detection value of the distance sensor (the theoretical detection value of the distance sensor when the sampling needle is coaxial with the center of the sample tube); is the radius of the current sample tube; is the actual detection value of the j-1th ranging sensor; A temperature measurement module, used to detect the temperature of a temperature measurement point in the current sample tube before stirring the current sample tube; A first warning module, used for issuing a warning when the actual center state value is greater than the preset center state value; The second calculation module calculates the actual temperature state value based on the following formula; ; is the actual temperature state value; M is the total number of temperature measurement points; is the temperature detection value of the i-th temperature measurement point; is the reference temperature of the current sample in the current sample tube; for The total number of temperature measurement points corresponding to a value greater than 0; for The total number of temperature measurement points corresponding to less than 0; The third calculation module is based on the following calculation formula: ; P is the first target power of the stepper motor; is the theoretical power of the stepper motor when stirring the current type of sample in the sample tube with a radius of d; is the logarithm with base 10, and e is a natural constant.

[0030] The beneficial effects of the above technical solution are: The full-spectrum flow cytometer can automatically transport multiple sample tubes to the bottom of the sampling needle 7 through the conveying device. Due to the actual position error (small error) of the conveying device or the single-tube mixing and cleaning device of the full-spectrum flow cytometer in actual long-term use, the position of the sampling needle 7 may have an error (small error) due to installation or other factors; therefore, each time sampling is performed, the sampling needle 7 is inserted into the current sample tube to a preset depth, and then the distance measuring sensor is controlled to perform several detections to determine the position state of the sampling needle 7 in the sample tube during current stirring. Then, due to the viscosity state of the sample liquid at different temperatures, the required stirring force is different. Therefore, the actual temperature state value is calculated based on the temperature measurement module, and then the appropriate first target power is determined based on the actual center state value, the actual temperature state value and the radius of the current sample tube. The actual power of the stepper motor when the current sample tube is stirred is controlled to be the first target power to ensure the stirring effect.

[0031] Considering the average radial center deviation state , and the deviation state of the distance measuring sensor position at adjacent circumferential positions , convenient according to Reliable adjustment power; Consider the average temperature deviation state , and the temperature distribution , convenient according to Reliable regulating power.

[0032] Embodiment 3, based on Embodiment 1 or 2, the swab liquid inlet 20 is connected to a liquid inlet tube, the liquid inlet tube is connected to an infusion pump, the infusion pump is connected to a cleaning liquid source, and further includes: The first acquisition module is used to acquire a theoretical power-hydraulic change curve of the infusion pump; the abscissa of the theoretical power-hydraulic change curve is the working power of the infusion pump, and the ordinate is the hydraulic pressure at the outlet of the inlet pipe; A second acquisition module is used to acquire the cleaning liquid source parameters; The third acquisition module is used to obtain sample parameters, including sample density and sample viscosity; The fourth acquisition module is used to obtain the historical cleaning parameters of the sampling needle 7 when the historical cleaning is qualified, and the historical cleaning parameters include: the historical hydraulic pressure of the liquid inlet pipe outlet, the historical sample parameters, and the historical parameters of the cleaning liquid source; A timing module, used to time the working time of the infusion pump; A third calculation module, used for calculating the actual sample cleaning state value in the sample tube to be cleaned based on the second acquisition module and the third acquisition module; The first screening module is used to screen the historical cleaning parameters of the sampling needle 7 when the target historical cleaning is qualified; the historical sample cleaning state value when the target historical cleaning of the sampling needle 7 is screened to be within the same state value range as the actual sample cleaning state value in the sample tube to be cleaned; initially, an initial cleaning test can be performed for different sample cleaning state values ​​to determine the minimum hydraulic pressure at the outlet of the liquid inlet tube when different sample cleaning state values ​​are qualified for cleaning, and then different sample cleaning state values ​​and the corresponding minimum hydraulic pressure are associated and stored. During the actual cleaning process, the infusion pump is first controlled to work so that the actual hydraulic pressure at the outlet of the liquid inlet tube is the corresponding minimum hydraulic pressure, and a cleaning test is performed. According to the cleaning test result (whether the cleaning result is qualified can be determined according to the grayscale change state of the sample tube before and after cleaning), the actual hydraulic pressure at the outlet of the liquid inlet tube is adjusted to determine the actual hydraulic pressure at the outlet of the liquid inlet tube when the cleaning is qualified; a fourth calculation module, configured to calculate a second target power based on the first screening module, the first calculation module, the first acquisition module, and the timing module; The second control module is used to control the actual working power of the infusion pump to be the second target power when cleaning the sample in the current sample tube to be cleaned.

[0033] Preferably, the third calculation module calculates based on the following formula: ; The actual sample cleaning status value in the sample tube to be cleaned The sample density in the sample tube to be cleaned currently obtained by the third obtaining module; is the density of the cleaning liquid source obtained by the second acquisition module; D is the inner diameter of the sampling needle; is the friction coefficient of the inner wall of the sampling needle; The viscosity of the cleaning liquid source obtained by the second obtaining module; The viscosity of the sample in the sample tube to be cleaned currently obtained by the third obtaining module; is the unit inner diameter; The fourth calculation module is calculated based on the following formula: ; is the second target power; The maximum value of the historical hydraulic pressure at the outlet of the liquid inlet pipe in the historical cleaning parameters when the target historical cleaning is qualified obtained by the first screening module; The minimum value of the historical hydraulic pressure at the outlet of the liquid inlet pipe among the historical cleaning parameters when the target historical cleaning is qualified obtained by the first screening module; for The length of time between the corresponding historical moment and the current moment; is the time attenuation coefficient of the infusion pump; The ordinate obtained based on the theoretical power-hydraulic change curve of the infusion pump is The corresponding working power of the infusion pump; K is the theoretical power of the infusion pump - the vertical coordinate in the hydraulic pressure change curve is The slope at for The corresponding historical moments and the duration of the corresponding historical moment; is the natural logarithm, and e is a natural constant.

[0034] The beneficial effects of the above technical solution are: The actual sample cleaning state value in the sample tube is determined based on the cleaning liquid source parameters and the sample parameters in the sample tube. The larger the actual sample cleaning state value in the sample tube, the easier it is to have cleaning residues and the larger the required cleaning hydraulic pressure. The historical cleaning parameters of the sampling needle 7 when the target historical cleaning is qualified are determined based on the actual sample cleaning state value in the sample tube to be cleaned (the historical sample cleaning state value of the sampling needle 7 when the target historical cleaning is qualified and the actual sample cleaning state value in the sample tube to be cleaned are within the same state value range). The historical cleaning parameters when the target historical cleaning is qualified are used as the basis, and the second target power is determined based on the theoretical initial power and hydraulic state of the infusion pump (theoretical power-hydraulic state change curve of the infusion pump). When the second control module controls the cleaning of the sample in the sample tube to be cleaned, the actual working power of the infusion pump is the second target power, thereby ensuring the selection of a suitable power of the infusion pump, ensuring the cleaning effect of the sample tube, and improving the cleaning efficiency.

[0035] Embodiment 4, on the basis of any one of Embodiments 1-3, as Figure 6 As shown, it also includes an integrated circuit, which includes: The monitoring device (which may be a voltage sensor for monitoring the working voltage of the motor) is used to monitor the working parameters of the motor. The monitoring device is electrically connected to the control device through the first auxiliary circuit, and the control device is electrically connected to the power supply through the second auxiliary circuit.

[0036] The first auxiliary circuit comprises: a first diode D1, wherein the anode of the first diode D1 is connected to the output end of the monitoring device, the cathode of the first diode D1 is connected in series with a fifth resistor R5 and a third resistor R3 in sequence, a first end of a fourth resistor R4 is connected to an end of the third resistor R3 away from the fifth resistor R5, and a second end of the fourth resistor R4 is grounded; A first capacitor C1, one end of which is grounded, and the other end of which is connected to a first end of a fourth resistor R4; A second resistor R2, one end of which is connected to the first end of the fourth resistor R4, the other end of the second resistor R2 is connected to the anode of the second diode D2, the cathode of the second diode D2 is connected to the first power supply V1, the anode of the second diode D2 is also grounded through the second capacitor C2, and the anode of the second diode D2 is also connected to the control device; The second auxiliary circuit comprises: A sixth field effect transistor U6, a source electrode connected to the second power supply V2, a gate electrode connected to the drain electrode of the sixth field effect transistor U6, and a drain electrode of the sixth field effect transistor U6 is grounded; A third field effect transistor U3, a source of which is connected to the second power supply V2, and a gate of which is connected to the gate of the sixth field effect transistor U6; A fourth field effect transistor U4, a source of which is connected to the second power supply V2, and a gate of which is connected to the gate of the third field effect transistor U3; A first field effect transistor U1, a source electrode of which is connected to a second power source V2, and a gate electrode of the first field effect transistor U1 is connected to a drain electrode of a fourth field effect transistor U4; A fifth field effect transistor U5, the gate of which is connected to the drain, the drain of the fifth field effect transistor U5 is connected to the drain of the third field effect transistor U3, the gate of the fifth field effect transistor U5 is connected to the gate of the second field effect transistor U2, the drain of the fifth field effect transistor U5 is also connected to the source of the third field effect transistor U3, the source of the second field effect transistor U2 is connected in series with the sixth resistor R6 and the seventh resistor R7 in sequence, and then connected to the second end of the eighth resistor R8, the first end of the eighth resistor R8 is grounded, the second end of the eighth resistor R8 is also connected to the control device, the first end of the first resistor R1 is connected to the second end of the eighth resistor R8, and the second end of the first resistor R1 is connected to the source of the fifth field effect transistor U5 and the drain of the power field effect transistor U7; The power field effect transistor U7 has a source connected to the second power supply V2 and a gate connected to the drain of the first field effect transistor U1 .

[0037] Among them, U6, U3, U4, and U1 are P-channel MOS transistors, and U5 and U7 are N-channel field effect transistors; The beneficial effects of the above technical solution are: D1 is used for rectification and voltage stabilization, R3, R4, and R5 are used for voltage regulation, C1 / R4 are used for noise reduction, and C2 and R2 are used for filtering. The above ensures that the monitoring signal of the monitoring device is reliably transmitted to the control device, making it convenient for the control device to control the motor according to the monitoring signal of the monitoring device, thereby ensuring the reliability of the motor.

[0038] U1, U7, and R6 are used for over-current protection, and U4, U3, and U6 are used for current adjustment.

[0039] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A single-tube mixing and cleaning structure, characterized in that: include: A mounting plate (9), to which a lifting mechanism is fixedly connected, and an eccentric mechanism is installed at the lifting end of the lifting mechanism; An eccentric needle seat (5), wherein the rotating end of the eccentric mechanism is connected to the eccentric needle seat (5), the sampling needle (7) is mounted on the eccentric needle seat (5), the sampling needle (7) passes through the mounting hole of the sampling needle swab (8), the sampling needle swab (8) is hollow inside and is provided with a swab liquid inlet (20) and a swab liquid outlet (23).

2. A single-tube mixing and cleaning structure according to claim 1, characterized in that: The eccentric mechanism comprises: a motor mounting block (14), a lifting end of the lifting mechanism being mounted with the motor mounting block (14), a motor (3) being mounted on the motor mounting block (14), an eccentric rotating shaft (4) being fixed on the output shaft of the motor (3), and the eccentric rotating shaft (4) being mounted on the eccentric needle seat (5) via a first bearing (21); An optical coupling baffle (16) is fixed on the eccentric rotating shaft (4), and a counting optical coupling (17) is mounted on the motor mounting block (14). When the optical coupling baffle (16) rotates, it passes through the counting optical coupling (17).

3. A single-tube mixing and cleaning structure according to claim 1, characterized in that: Also includes: A first retaining shaft (24), wherein the first retaining shaft (24) is fixed on the sampling needle swab (8), a fixing sleeve (11) is fixedly arranged on the mounting plate (9), and the first retaining shaft (24) is inserted into the fixing sleeve (11); Two guide retaining shafts (6), the two guide retaining shafts (6) are fixed on the sampling needle swab (8), the two guide retaining shafts (6) pass through the second bearing (12), and the second bearing (12) is installed on the eccentric needle seat (5).

4. A single-tube mixing and cleaning structure according to claim 2, characterized in that: The lifting mechanism comprises: a fixed guide rail (1), the fixed guide rail (1) is fixedly connected to a mounting plate (9), a sliding block (2) is slidably connected to the fixed guide rail (1) along an up-down direction, and a motor mounting block (14) is mounted on the sliding block (2); the sliding block (2) is driven by a translation driving mechanism to slide on the fixed guide rail (1).

5. A single-tube mixing and cleaning structure according to claim 2, characterized in that: Also includes: A tension spring fixing piece (15) is fixedly connected to the motor mounting block (14); a tension spring fixing pin shaft (18) is fixedly arranged on the eccentric needle seat (5); and two ends of the guide holding tension spring (13) are respectively connected to the tension spring fixing piece (15) and the tension spring fixing pin shaft (18).

6. A single-tube mixing and cleaning structure according to claim 1, characterized in that: Also includes: A detection sleeve, which is detachably mounted on the outer side of the upper portion of the sampling needle (7), and a distance measuring sensor is arranged on the outer peripheral side of the detection sleeve, and the distance measuring sensor is used to detect the vertical distance between the distance measuring sensor and the side wall of the current sample tube. Each time sampling is performed, the sampling needle (7) is inserted into the current sample tube to a preset depth, and then the distance measuring sensor is controlled to perform several detections; A first calculation module, used for calculating an actual center state value based on a detection value of a ranging sensor; A temperature measurement module, used to detect the temperature of a temperature measurement point in the current sample tube before stirring the current sample tube; A second calculation module, used for calculating the actual temperature state value based on the temperature measurement module; A third calculation module, used for calculating a first target power of the stepper motor when stirring the current sample tube based on the first calculation module and the second calculation module; The first control module is used to control the actual power of the stepper motor to be the first target power when the sample tube is currently being stirred.

7. A single-tube mixing and cleaning structure according to claim 6, characterized in that: A first calculation module calculates the actual center state value based on the following formula; ； is the actual center state value; is the actual detection value of the jth ranging sensor; N is the total number of ranging sensors; is the reference detection value of the distance measuring sensor; is the radius of the current sample tube; is the actual detection value of the j-1th ranging sensor; A temperature measurement module, used to detect the temperature of a temperature measurement point in the current sample tube before stirring the current sample tube; The second calculation module calculates the actual temperature state value based on the following formula; ； is the actual temperature state value; M is the total number of temperature measurement points; is the temperature detection value of the i-th temperature measurement point; is the reference temperature of the current sample in the current sample tube; for The total number of temperature measurement points corresponding to a value greater than 0; for The total number of temperature measurement points corresponding to less than 0; The third calculation module is based on the following calculation formula: ； P is the first target power of the stepper motor; is the theoretical power of the stepper motor when stirring the current type of sample in the sample tube with a radius of d; is the logarithm with base 10, and e is a natural constant.

8. The single-tube mixing and cleaning structure according to claim 1, characterized in that: The swab liquid inlet (20) is connected to a liquid inlet tube, the liquid inlet tube is connected to an infusion pump, the infusion pump is connected to a cleaning liquid source, and further comprises: The first acquisition module is used to acquire a theoretical power-hydraulic change curve of the infusion pump; the abscissa of the theoretical power-hydraulic change curve is the working power of the infusion pump, and the ordinate is the hydraulic pressure at the outlet of the inlet pipe; A second acquisition module is used to acquire the cleaning liquid source parameters; The third acquisition module is used to obtain sample parameters, including sample density and sample viscosity; A fourth acquisition module is used to acquire historical cleaning parameters of the sampling needle (7) when the historical cleaning is qualified, the historical cleaning parameters including: historical hydraulic pressure at the outlet of the liquid inlet pipe, historical sample parameters, and historical parameters of the cleaning liquid source; A timing module, used to time the working time of the infusion pump; A third calculation module, used for calculating the actual sample cleaning state value in the sample tube to be cleaned based on the second acquisition module and the third acquisition module; The first screening module is used to screen the historical cleaning parameters of the sampling needle (7) when the target historical cleaning is qualified; the historical sample cleaning state value when the target historical cleaning of the sampling needle (7) is screened to be qualified and the actual sample cleaning state value in the sample tube to be cleaned is within the same state value range; a fourth calculation module, configured to calculate a second target power based on the first screening module, the first calculation module, the first acquisition module, and the timing module; The second control module is used to control the actual working power of the infusion pump to be the second target power when cleaning the sample in the current sample tube to be cleaned.

9. A single-tube mixing and cleaning structure according to claim 8, characterized in that: The third calculation module is based on the following calculation formula: ； is the actual sample cleaning status value in the sample tube to be cleaned; The sample density in the sample tube to be cleaned currently obtained by the third obtaining module; is the density of the cleaning liquid source obtained by the second acquisition module; D is the inner diameter of the sampling needle; is the friction coefficient of the inner wall of the sampling needle; The viscosity of the cleaning liquid source obtained by the second obtaining module; The viscosity of the sample in the sample tube to be cleaned currently obtained by the third obtaining module; is the unit inner diameter; The fourth calculation module is calculated based on the following formula: ； is the second target power; The maximum value of the historical hydraulic pressure at the outlet of the liquid inlet pipe in the historical cleaning parameters when the target historical cleaning is qualified obtained by the first screening module; The minimum value of the historical hydraulic pressure at the outlet of the liquid inlet pipe among the historical cleaning parameters when the target historical cleaning is qualified obtained by the first screening module; for The length of time between the corresponding historical moment and the current moment; is the time attenuation coefficient of the infusion pump; The ordinate obtained based on the theoretical power-hydraulic change curve of the infusion pump is The corresponding working power of the infusion pump; K is the theoretical power of the infusion pump - the vertical coordinate in the hydraulic pressure change curve is The slope at for The corresponding historical moments and the duration of the corresponding historical moment; is the natural logarithm, and e is a natural constant.

10. The single-tube mixing and cleaning structure according to claim 1, characterized in that: It also includes integrated circuits, which include: The monitoring device is used to monitor the working parameters of the motor. The monitoring device is electrically connected to the control device through a first auxiliary circuit, and the control device is electrically connected to the power supply through a second auxiliary circuit.

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