A strain of Lactiplantibacillus plantarum YW99, its cell lysate and their antibacterial applications

Abstract

The present invention relates to the field of microorganisms, and specifically, to a Lactiplantibacillus plantarum YW99, its cell lysate, and their antibacterial applications. Lactiplantibacillus plantarum ( Lactiplantibacillus plantarum ) YW99 has been deposited in the General Microbiological Center of the China General Microbiological Culture Collection Center, with the deposit number of CGMCC No. 33160. The cell lysate of Lactiplantibacillus plantarum YW99 is prepared by a series of operations after the YW99 strain is heat-inactivated. It has good safety, will not cause toxic and side effects to the human body, has a certain function of improving the body's immunity, and can show different degrees of antibacterial ability against Candida albicans, Gardnerella, Pseudomonas aeruginosa, Aspergillus brasiliensis and other strains by changing the concentration of the YW99 cell lysate. It has good application prospects in female health products, hygiene products, daily chemical products, and disinfection products.

Description

Technical Field

[0001] The present invention relates to the field of microorganisms, and specifically, to a Lactiplantibacillus plantarum YW99, its cell lysate, and the antibacterial applications of both. Background Art

[0002] With the improvement of people's living standards, people not only satisfy their basic food and clothing needs but also pay increasing attention to health. Pathogenic bacteria are bacteria that cause human diseases. They are tiny in size and can enter the human body through various channels, parasitize, multiply, and cause diseases in the human body.

[0003] Pathogenic bacteria such as Candida albicans and Gardnerella vaginalis can attach to the skin or mucous membranes of the human body. When the balance of the human body's flora is disrupted, these pathogenic bacteria will multiply in large numbers and cause diseases. They are the main pathogenic bacteria of vaginitis and the primary factor leading to many common female health problems. However, there are currently not many targeted effective substances, and the effects are not obvious. In the common prior art on the market, most feminine hygiene products with antibacterial and bacteriostatic functions use antibacterial agents containing metals, antibiotics, or organic substances. First, during use, these antibacterial agents cause significant irritation to the skin and mucous membranes, bringing certain discomfort to the applicable population, or having defects such as carcinogenicity, teratogenicity, and short shelf life, affecting physical and mental health and normal life. Second, the antibacterial agents will be lost, which will not only reduce the antibacterial efficiency and duration of feminine hygiene products but also cause environmental pollution.

[0004] Pseudomonas aeruginosa, also known as green pus bacillus, is an opportunistic pathogen that is resistant to a variety of antibiotics. It is a common contaminant of food and cosmetics. There is currently no good antibacterial drug for it, and mainly the method of using antibiotics is adopted to inhibit it. However, Pseudomonas aeruginosa is extremely likely to develop drug resistance, so there are few effective antibiotics. Moreover, overuse will cause the bacterial drug resistance to become more serious, posing a potential threat to human health and the ecological environment.

[0005] Aspergillosis is a chronic mycosis caused by the infection of Aspergillus, which can invade the skin, mucous membranes, eyes, external auditory canals, nose, nasal sinuses, bronchi, lungs, gastrointestinal tract, nervous system, or bones, and in severe cases, lead to septicemia. Aspergillus brasiliensis is the most common Aspergillus species of fungi, which will spread in production sites or hospitals through improperly cleaned equipment. After being infected, it will cause severe lung lesions. The common inhibitory drugs are broad-spectrum disinfectants and antifungal agents. The former is applied to industrial disinfection, and the latter is applied to treat the infected.

[0006] Natural antibacterial preservatives mainly come from animal, plant, and microbial extracts, etc., which are safer for the human body and meet the requirements of green environmental protection. Due to its safety, lactic acid bacteria have been widely used in the food field, and bacteriocin, the metabolite of lactic acid bacteria, is a natural antibacterial agent that combines safety and antibacterial activity at present and has attracted much attention. For example, reuterin has extremely strong antibacterial activity against Helicobacter pylori, and nisin has a certain inhibitory effect on some foodborne Gram-positive pathogenic bacteria. However, in the fields of female health products, sanitary products, daily chemical products, disinfection products, etc., it still cannot meet the increasingly diverse market demands and the antibacterial needs of female populations. This not only restricts the high-quality development of various industries but also affects people's physical health. Therefore, there is an urgent need to find a new, natural, safe, broad-spectrum antibacterial, and highly efficient antibacterial substance to be applied in the fields of female health products, sanitary products, daily chemical products, disinfection products, etc., to promote the upgrading of the industry. Summary of the Invention

[0007] In view of the deficiencies in the above-mentioned prior art, the present application provides a Lactiplantibacillus plantarum YW99 and a cell lysate of Lactiplantibacillus plantarum YW99.

[0008] The first object of the present invention is to provide an application of Lactiplantibacillus plantarum in the preparation of a product with antibacterial function. The Lactiplantibacillus plantarum is Lactiplantibacillus plantarum YW99, and its taxonomic name is Lactiplantibacillus plantarum , which was deposited at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on December 23, 2024, with the deposit number CGMCC No. 33160. The above-mentioned product is one or more of female health products, sanitary products, daily chemical products, and disinfection products.

[0009] Furthermore, the above-mentioned antibacterial function at least includes one of the following: (1) inhibiting the growth of Candida albicans; (2) inhibiting the growth of Gardnerella vaginalis; (3) inhibiting the growth of Pseudomonas aeruginosa; (4) inhibiting the growth of Aspergillus brasiliensis.

[0010] Furthermore, the above-mentioned product at least meets one of the following: (1) the sanitary product is a gynecological sanitary product, and the gynecological sanitary product is a sanitary napkin or a gel product; (2) the daily chemical product is an oral health care product, and the oral health care product is one or more of a mouthwash, mouthwash powder, toothpaste, tooth powder, and oral spray; (3) the disinfection product is a skin disinfection product, and the skin disinfection product is one or more of a tissue, wet wipe, antibacterial liquid product, wet toilet paper, and facial mask.

[0011] The second object of the present invention is to provide an application of a cell lysate solution of Lactiplantibacillus plantarum YW99 in the preparation of a product with antibacterial function. The cell lysate solution of Lactiplantibacillus plantarum YW99 is prepared from Lactiplantibacillus plantarum YW99 with the preservation number of CGMCC No. 33160. The cell lysate solution of Lactiplantibacillus plantarum YW99 satisfies at least one of the following: (1) The concentration of the cell lysate solution of Lactiplantibacillus plantarum YW99 is 10% (W / V), and it contains at least 10 10 CFU / mL of inactivated Lactiplantibacillus plantarum YW99 cells; (2) It contains the metabolites lactic acid and acetic acid of Lactiplantibacillus plantarum YW99, with the lactic acid content ≥ 22 mg / 100 mL and the acetic acid content ≥ 5 mg / 100 mL; (3) It contains the metabolite flavonoids of Lactiplantibacillus plantarum YW99, with the flavonoid content ≥ 52 mg / mL; (4) It contains the metabolite amino acids of Lactiplantibacillus plantarum YW99, with the amino acid content ≥ 70 μmol / mL; (5) It contains the metabolite polysaccharides of Lactiplantibacillus plantarum YW99, with the polysaccharide content ≥ 700 mg / L; The product is one or more of female health products, hygiene products, daily chemical products, and disinfection products.

[0012] Furthermore, the above antibacterial function includes at least one of the following: (1) Inhibiting the growth of Candida albicans; (2) Inhibiting the growth of Gardnerella vaginalis; (3) Inhibiting the growth of Pseudomonas aeruginosa; (4) Inhibiting the growth of Aspergillus brasiliensis.

[0013] Furthermore, the above product satisfies at least one of the following: (1) The hygiene product is a gynecological hygiene product, and the gynecological hygiene product is a sanitary napkin or a gel product; (2) The daily chemical product is an oral health care product, and the oral health care product is one or more of mouthwash, mouthwash powder, toothpaste, tooth powder, and oral spray; (3) The disinfection product is a skin disinfection product, and the skin disinfection product is one or more of tissue paper, wet wipes, antibacterial liquid products, wet toilet paper, and facial masks.

[0014] Compared with the prior art, the present invention has the following advantages:

[0015] (1) The present application provides a strain of Lactiplantibacillus plantarum YW99 and the cell lysate of Lactiplantibacillus plantarum YW99. This strain is isolated and screened from Tibetan kefir grains and is a probiotic with strong antibacterial function. It has certain application value in the preparation of female health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), and disinfection products. The cell lysate of Lactiplantibacillus plantarum YW99 is prepared by heat inactivation method. The preparation method is simple and efficient. The obtained cell lysate of Lactiplantibacillus plantarum YW99 has good inhibitory effects on the growth of pathogenic bacteria such as Candida albicans, Gardnerella vaginalis, Pseudomonas aeruginosa, and Aspergillus brasiliensis. Moreover, this preparation method can increase the concentration of effective substances in the cell lysate of Lactiplantibacillus plantarum YW99 and enhance the antibacterial effects of the cell lysate of Lactiplantibacillus plantarum YW99 against Candida albicans, Gardnerella vaginalis, Pseudomonas aeruginosa, and Aspergillus brasiliensis.

[0016] (2) Based on the function of the cell lysate of Lactiplantibacillus plantarum YW99, the present application applies Lactiplantibacillus plantarum YW99 or the cell lysate of Lactiplantibacillus plantarum YW99 to fields such as female health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), and disinfection products, providing a natural and highly safe antibacterial option for the market and consumers. Compared with chemical antibacterial drugs, it has characteristics such as naturalness, safety, non-toxicity, and good biocompatibility. On the one hand, it reduces the irritation to the skin and mucous membranes of users and the probability of carcinogenicity and teratogenicity, and on the other hand, it meets the requirements of green environmental protection, so it has a wider scope of application. Compared with antibiotic agents, there is no hidden danger of bacterial drug resistance caused by overuse. Compared with traditional probiotic preparations, the cell lysate of Lactiplantibacillus plantarum YW99 has no pathogenicity and the metabolite release is more complete. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is the effect diagram of the cell lysate of YW99 strain in Test Example 1 of the present application inhibiting Candida albicans;

[0018] Figure 2 It is the effect diagram of the cell lysate of YW99 strain in Test Example 1 of the present application inhibiting Gardnerella vaginalis;

[0019] Figure 3 It is the effect diagram of the cell lysate of YW99 strain in Test Example 1 of the present application inhibiting Pseudomonas aeruginosa;

[0020] Figure 4 It is the MIC experimental result of the cell lysate of YW99 strain in Test Example 2 of the present application against Candida albicans;

[0021] Figure 5 It is the anti-corrosion test result of the YW99 antibacterial gel in Test Example 4 of the present application. Detailed implementation manners

[0022] The present invention will be further described below in conjunction with specific embodiments. The following embodiments are only used to more clearly illustrate the technical solutions of the present invention and cannot be used to limit the protection scope of the present invention. Those not described in detail in this patent application for the present invention can be understood as common general knowledge in the art.

[0023] With the development of society, consumers have more diverse requirements for female health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), and disinfection products with antibacterial and bacteriostatic functions. A large number of studies have shown that probiotics have a variety of probiotic functions, such as regulating the intestinal flora, enhancing immunity, promoting nutrient absorption, reducing blood sugar, antioxidation, and reducing the risk of diseases. As a preparation of inanimate microorganisms and / or their components beneficial to the health of the host, lactic acid bacteria cell lysates mainly include the metabolites of probiotic bacteria and the soluble substances produced after the bacteria are eliminated and decomposed, such as bacteriocins, antimicrobial peptides, organic acids, and exopolysaccharides, etc. Moreover, compared with probiotics, they are more stable, have higher safety, are also suitable for some special populations such as newborns and sensitive populations, and are not interfered or inhibited by antibiotics. However, at present, most probiotics and lactic acid bacteria cell lysates are used in the food field and rarely applied to the fields of disinfection and antibacterial. Therefore, this application aims to apply probiotics and / or lactic acid bacteria cell lysates to industries such as female health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), and disinfection products, so as to provide consumers with a new, natural, safe, broad-spectrum antibacterial, and highly efficient antibacterial and bacteriostatic product.

[0024] The Lactiplantibacillus plantarum used in the present invention is: Lactiplantibacillus plantarum YW99, the preservation date is December 23, 2024, the preservation unit is the General Microbiology Center of the China Microbial Culture Collection Center, and the taxonomic name is Lactiplantibacillus plantarum ( Lactiplantibacillus plantarum ), and the preservation number is CGMCC No. 33160.

[0025] Unless otherwise specified, the experimental methods in the following embodiments are all conventional methods; unless otherwise specified, the test materials and culture media used in the following embodiments are all obtained from commercial channels.

[0026] MRS medium: Glucose 20.0 g / L, peptone 10.0 g / L, beef extract 10.0 g / L, yeast extract 5.0 g / L, sodium acetate 5.0 g / L, diammonium citrate 2.0 g / L, dipotassium hydrogen phosphate 2.0 g / L, magnesium sulfate 0.2 g / L, manganese sulfate 0.05 g / L, 1.0 g / L Tween-80, made up to 1,000 mL with distilled water. For solid medium, 2% agar is added, the pH value is 6.2 - 6.4, and autoclaved at 115 °C for 15 min.

[0027] Sabouraud Dextrose Agar Medium (SDA): 40.0 g of glucose, 5.0 g of animal tissue gastric digest, 5.0 g of tryptone, 15.0 g of agar, made up to 1,000 mL with distilled water, pH 5.4 - 5.8, autoclaved at 121 °C for 15 min.

[0028] Potato Dextrose Agar (PDA): 200.0 g of potato extract, 20.0 g of glucose, 20.0 g of agar, made up to 1,000 mL with distilled water, pH 5.4 - 5.8, autoclaved at 121 °C for 15 min.

[0029] Example 1 Preparation of YW99 Cell Lysate

[0030] Pick an appropriate amount of Lactiplantibacillus plantarum YW99 isolated from Tibetan kefir grains to prepare YW99 cell lysate. The specific method is as follows:

[0031] S1. Inoculate Lactiplantibacillus plantarum YW99 into MRS medium for full activation, culture at 37 °C for 24 h until the late logarithmic phase to obtain the YW99 activated strain; then inactivate at 105 °C for 20 min, centrifuge at 8,000 r / min for 3 min, and take the supernatant to obtain the YW99 cell lysate stock solution, which is stored at -20 °C for later use;

[0032] S2. Pre-freeze the YW99 cell lysate stock solution at -80 °C for 3 h, and then perform freeze-drying operation for 24 h to obtain the dry powdered YW99 cell lysate, which is stored for later use.

[0033] Example 2 Preparation of YW99 Cell Lysate Solution

[0034] Resuspend the YW99 cell lysate obtained in Example 1 to obtain a 10% (W / V) YW99 cell lysate solution. Conduct component detection on it and find that the 10% YW99 cell lysate solution contains metabolites such as lactic acid, acetic acid, flavonoids, amino acids, and polysaccharides. The content detection results of the main metabolites are shown in Table 1.

[0035] Table 1 Component Contents in 10% YW99 Cell Lysate Solution

[0036]

[0037] In some specific embodiments, a 10% YW99 cell lysate solution can be added as a raw material to female health products, sanitary products, daily chemical products (including but not limited to dental products, cosmetics), and disinfection products. In other specific embodiments, the pH of the finally obtained 10% YW99 cell lysate solution is in the range of 3.5 - 4.0, and the contents of bacteria and metabolites both at least meet the following: inactivated Lactiplantibacillus plantarum YW99 bacteria ≥ 10 10 CFU / mL, lactic acid ≥ 22 mg / 100 mL, acetic acid ≥ 5 mg / 100 mL, flavonoids ≥ 52 mg / mL, amino acids ≥ 70 μmol / mL, polysaccharides ≥ 700 mg / L. In some other specific embodiments, the YW99 cell lysate solution may also contain the above metabolites alone or simultaneously to meet different application requirements.

[0038] Test Example 1 Qualitative Detection of the Bacteriostatic Ability of YW99 Cell Lysate

[0039] In this test example, the YW99 cell lysate prepared in Example 1 was formulated into a 10% (W / V) solution for the determination of bacteriostatic performance. Activated Candida albicans ATCC 10231, Gardnerella vaginalis, and Pseudomonas aeruginosa ATCC 9027 were separately taken and formulated into bacterial solutions with a concentration of 1×10 8 CFU / mL. 100 μL of each bacterial solution was taken and spread on the corresponding solid medium. A puncher with an inner diameter of 6 mm was used to punch holes in the solid medium, with 4 or 5 holes per dish. Among them, 2 holes were used as positive controls (+: 50 μg / mL ampicillin) and negative controls (-: physiological saline). Different concentrations of the YW99 cell lysate solution were taken and 100 μL of each was injected into the holes. After culturing at a certain temperature for a period of time, the diameter of the bacteriostatic zone was measured with a vernier caliper, and the results were analyzed as follows.

[0040] 1. Detection of the inhibitory ability of YW99 cell lysate against Candida albicans by the bacteriostatic zone method

[0041] Using Candida albicans as the experimental bacterium and adopting the bacteriostatic zone method, in this test example, the YW99 cell lysate prepared in Example 1 was formulated into 10% (W / V) and 20% (W / V) solutions, and the bacteriostatic performances were respectively determined to measure their bacteriostatic abilities against Candida albicans. The solid medium was SDA solid medium, and it was cultured at 35°C for 16 h - 18 h. The results of the bacteriostatic zone of the 10% YW99 cell lysate solution are as Figure 1 shown. The specific experimental data are shown in Table 2, and the diameters of the bacteriostatic zones were 1.7 cm and 2.0 cm respectively, indicating that the YW99 cell lysate has an inhibitory effect on Candida albicans.

[0042] Table 2 Diameters of the Bacteriostatic Zones of YW99 Cell Lysate against Candida albicans

[0043]

[0044] The above results indicate that the YW99 cell lysate and the resuspended solution exhibit an obvious inhibitory effect on Candida albicans. As the concentration of the YW99 cell lysate solution increases, its inhibitory effect on Candida albicans also increases significantly.

[0045] 2. Detection of the inhibitory ability of YW99 cell lysate against Gardnerella vaginalis by the inhibition zone method

[0046] The medium was BHI solid medium: purchased from Qingdao Haibo Biotechnology Co., Ltd., and obtained by autoclaving at 121 °C for 15 min. It was cultured at 35 °C for 24 h - 48 h. The results of the inhibition zone of the 10% YW99 cell lysate solution are as Figure 2 shown. The diameter of the inhibition zone was 1.2 cm ± 0.2 cm, indicating that the YW99 cell lysate has an inhibitory effect on Gardnerella vaginalis.

[0047] 3. Detection of the inhibitory ability of YW99 cell lysate against Pseudomonas aeruginosa by the inhibition zone method

[0048] The solid medium was LB agar medium, and it was cultured at 37 °C for 16 h - 18 h. The results of the inhibition zone of the 10% YW99 cell lysate solution are as Figure 3 shown. The diameter of the inhibition zone was 1.6 cm ± 0.06 cm, indicating that the YW99 cell lysate has a good inhibitory effect on Pseudomonas aeruginosa.

[0049] Experimental Example 2 Quantitative detection of the antibacterial ability of YW99 cell lysate

[0050] In this experimental example, the minimum inhibitory concentration (MIC) of the YW99 cell lysate prepared in Example 1 against Candida albicans was determined. The experiment was carried out by the microbroth dilution method, in accordance with the "National Health Industry Standard of the People's Republic of China" (WS / T639 - 2018). Candida albicans bacterial liquid with a concentration of 1×10 6 CFU / mL was added to a 96 - well plate at an addition amount of 100 μL / well. The medium was used as the growth control group. In the experimental group, YW99 cell lysate solutions with different concentrations were prepared, including: 2.5% YW99 cell lysate solution, 5% YW99 cell lysate solution, 10% YW99 cell lysate solution, 20% YW99 cell lysate solution, and 3 parallels were set for each group. The incubation conditions were incubation at 25 °C - 40 °C for 16 h - 24 h. After the incubation, the turbidity of the liquid in the 96 - well plate was observed to determine the lowest concentration at which the lactic acid bacteria cell lysate inhibits the visible growth of pathogenic bacteria as the MIC. The experimental results are as Figure 4As shown, by visual observation, compared with the control group, as the concentration of the YW99 cell lysate solution increased, the inhibitory effect on Candida albicans gradually increased. When the concentration of the YW99 cell lysate solution was 5%, the suspension in the well was clear and no colonies were formed at the bottom of the well, indicating that the YW99 cell lysate had an obvious inhibitory effect on Candida albicans, and its minimum inhibitory concentration was 5%.

[0051] Test Example 3 Detection of the antibacterial ability of YW99 cell lysate using standard methods

[0052] Since the YW99 cell lysate has a strong antibacterial function against Candida albicans and Gardnerella vaginalis and has the basis and prospects for application in the preparation of products such as disposable hygiene products, this test example was carried out in accordance with GB 15979-2002 Hygiene Standards for Disposable Hygiene Products to verify its antibacterial characteristics as a raw material for antibacterial products.

[0053] In accordance with the requirements of GB15979-2002, this test example determined the inhibition rate of a 10% YW99 cell lysate solution against Candida albicans at different action times. Five tubes each of 5 mL of the test sample (10% YW99 cell lysate solution) and 5 mL of the control solution (physiological saline) were taken. 100 μL of a Candida albicans bacterial suspension was added to each sample solution and control solution and mixed evenly. After mixing, timing began. At 2 min, 5 min, 10 min, and 20 min of action, the sample solution and the control solution were diluted and spread for counting, and each group of tests was set up with one repeat. The inhibition rate at different action times was calculated according to the following formula.

[0054]

[0055] In the formula: A is the average number of colonies in the control solution, and B is the average number of colonies in the test sample.

[0056] According to the evaluation criteria of GB 15979-2002, if the inhibition rate is ≥50% - 90%, the tested product has an antibacterial effect, and if the inhibition rate is ≥90%, it indicates that the tested product has a strong antibacterial effect. The test results are shown in Table 3. It can be seen from Table 3 that the 10% YW99 cell lysate solution showed a strong antibacterial effect on Candida albicans at 2 min, 5 min, 10 min, and 20 min, and had an inhibitory effect at 2 min of action.

[0057] Table 3 Inhibition rate of YW99 cell lysate

[0058]

[0059] The results in Table 3 show that adding YW99 cell lysate as a raw material in the process of preparing disposable sanitary products can improve the antibacterial effect of the products. In other specific embodiments, the disposable sanitary products can be various daily necessities that are discarded after one use, directly or indirectly contact with the human body, and are used for the purpose of achieving human physiological hygiene or health care (antibacterial or bacteriostatic), and the product form can be solid or liquid, including but not limited to paper towels, wet wipes, sanitary wet wipes, women's menstrual hygiene products, excrement sanitary products such as diapers, condoms.

[0060] Example 3 Application of YW99 Cell Lysate in Female Health Products and Sanitary Products

[0061] Since Lactiplantibacillus plantarum YW99 and / or Lactiplantibacillus plantarum YW99 cell lysate have strong antibacterial effects, they are added as raw materials in suitable products to play an antiseptic role. In some specific embodiments, Lactiplantibacillus plantarum YW99 and / or Lactiplantibacillus plantarum YW99 cell lysate can be directly or indirectly added to female health products to inhibit the growth of Candida albicans, Gardnerella vaginalis, and Pseudomonas aeruginosa. In other specific embodiments, Lactiplantibacillus plantarum YW99 and / or YW99 cell lysate can also be added to sanitary products, including but not limited to gynecological sanitary products such as sanitary napkins and gel products.

[0062] This example provides an application of YW99 cell lysate in female health products, specifically involving the application of YW99 cell lysate in the preparation of gel-like gynecological sanitary products.

[0063] A YW99 antibacterial gel product, the preparation method is specifically as follows: add 3% carbomer (carbomer 934p powder produced by Anhui Newman Fine Chemical Co., Ltd.) to the 10% YW99 cell lysate solution prepared in Example 2, slowly stir to completely dissolve it, let it stand for a period of time to fully hydrate, and adjust the pH to 4.2 - 4.5 to obtain the YW99 antibacterial gel.

[0064] Test Example 4 Detection of Antibacterial and Antiseptic Properties of YW99 Antibacterial Gel

[0065] This test example detects the antibacterial and antiseptic properties of the YW99 antibacterial gel prepared in Example 3 against Candida albicans, Pseudomonas aeruginosa, and Aspergillus brasiliensis. Candida albicans was cultured with Sabouraud dextrose agar medium (SDA), and Aspergillus brasiliensis was cultured with potato dextrose agar (PDA), and the concentration of pathogenic bacteria was 5×10 5 CFU / mL - 4.5×10 6CFU / mL. The test method is carried out in accordance with the international standard ISO 11930, and the detection period is 28 days. The evaluation criteria are shown in Table 4.

[0066] Table 4 Evaluation Criteria

[0067]

[0068] The antibacterial effect determination results of YW99 antibacterial gel are as Figure 5 shown. In the figure, T0, T7, T14, and T28 represent the sampling times on the 0th, 7th, 14th, and 28th days respectively, and N0, N7, N14, and N28 in the figure represent the total number of bacteria in the samples at different sampling times. The results show that on the 14th day of cultivation, the viable counts of Candida albicans and Aspergillus brasiliensis in YW99 antibacterial gel are less than 10. According to the formula in Test Example 3, the logarithmic reduction values R14 of the viable bacteria amounts are 4.6 and 4.6 respectively, and the experimental results meet Standard B. On the 28th day of cultivation, the viable counts of Pseudomonas aeruginosa, Candida albicans, and Aspergillus brasiliensis in the mixed gel are all less than 10. According to the formula in Test Example 3, the logarithmic reduction values R28 of the viable bacteria amounts are 5.7, 4.6, and 4.6 respectively.

[0069] Combining the results on the 7th, 14th, and 28th days with Standard B for comparison, it can be seen that YW99 antibacterial gel meets Standard B in the international standard (ISO 11930), that is, it has an antiseptic and antibacterial effect on Pseudomonas aeruginosa, Candida albicans, and Aspergillus brasiliensis.

[0070] In summary, it can be seen that YW99 cell lysate can be used as a raw material for antibacterial products in female health products to inhibit or prevent the reproduction of pathogenic bacteria such as Pseudomonas aeruginosa, Candida albicans, and Aspergillus brasiliensis, and exert an antibacterial effect.

[0071] Example 4 Application of YW99 Cell Lysate in Daily Chemical Products and Disinfection Products

[0072] Since there are various microbial flora such as bacteria and fungi naturally present in the human oral cavity, and it has suitable temperature and humidity, which is extremely easy to promote the reproduction of microorganisms. In some specific embodiments, Lactiplantibacillus plantarum YW99 and / or YW99 cell lysate can be added to daily chemical products, including but not limited to oral health care products such as mouthwash, mouthwash powder, toothpaste, tooth powder, oral spray, etc.

[0073] In some other specific embodiments, Lactiplantibacillus plantarum YW99 and / or the cell lysate of YW99 can also be added to disinfection products, including but not limited to disinfectants, skin disinfection products (paper towels, wet wipes, antibacterial liquid products, wet toilet paper), etc. In some specific embodiments, the final dosage form of the antibacterial product added with Lactiplantibacillus plantarum YW99 and / or the cell lysate of Lactiplantibacillus plantarum YW99 can be any one of aerosol, spray, film, ointment, suppository, paste, solution, gel, injection, lotion.

[0074] Based on the ideal embodiments of the present invention as the inspiration, through the above description, relevant personnel can completely make various changes and modifications without departing from the technical idea of this invention. The technical scope of this invention is not limited to the content in the specification, and the technical scope must be determined according to the scope of the claims.

Claims

1. An application of Lactobacillus plantarum in preparing a product with antibacterial function, characterized in that: The plant lactobacillus is plant lactobacillus YW99, which is classified and named Lactiplantibacillus plantarum , deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on December 23, 2024, with the deposit number CGMCC No.33160; the antibacterial function is to inhibit the growth of Candida albicans, Gardnerella, Pseudomonas aeruginosa and Aspergillus brasiliensis; the product is one or more of sanitary products, daily chemical products or disinfection products; the application is to use the cell lysate of Lactobacillus plantarum YW99 to prepare products with antibacterial function.

2. The use according to claim 1, characterized in that: The cell lysate of Lactobacillus plantarum YW99 is a dry powdered cell lysate or a cell lysate solution, and the cell lysate solution satisfies at least one of the following conditions: (1) The concentration of the Lactobacillus plantarum YW99 cell lysate solution is 10% (W / V), including at least 10 10 CFU / mL of inactivated Lactobacillus plantarum YW99 bacteria; (2) Lactic acid and acetic acid, metabolites of Lactobacillus plantarum YW99, wherein the lactic acid content is ≥22 mg / 100 mL and the acetic acid content is ≥5 mg / 100 mL; (3) including flavonoids metabolites of Lactobacillus plantarum YW99, wherein the flavonoid content is ≥ 52 mg / mL; (4) Amino acids comprising metabolites of Lactobacillus plantarum YW99, wherein the amino acid content is ≥ 70 µmol / mL; (5) A polysaccharide comprising a metabolite of Lactobacillus plantarum YW99, wherein the content of the polysaccharide is ≥ 700 mg / L.

3. The use according to claim 1, characterized in that: The product meets at least one of the following requirements: (1) The sanitary products are gynecological sanitary products, and the gynecological sanitary products are sanitary napkins and gel products; (2) The daily chemical product is an oral health care product, and the oral health care product is one or more of mouthwash, mouthwash powder, toothpaste, tooth powder, and mouth spray; (3) The disinfectant is a skin disinfectant, which is one or more of paper towels, wet wipes, and antibacterial liquid products.

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