Application of plant-derived active substance DMPA in prevention and treatment of tomato leaf tier adult

Abstract

This invention discloses the application of a plant-derived active substance, DMPA, in the control of adult tomato leafminer moths, belonging to the field of pest control. The invention's research found that 2,2-dimethyl-4-pentenoic acid, the main volatile gaseous component of *Nepenthes*, has an excellent control effect on tomato leafminer moths, achieving a concentration of 1×10⁻⁶. ‑4 A dosage of 2 × 10⁻⁶ liters per cubic meter is lethal. ‑4 At a dosage of 1 liter per cubic meter, the lethality rate reaches approximately 70%, 4 × 10 ‑4 With a dosage of approximately 100% per cubic meter, it can be used to control tomato leafminer in greenhouses.

Description

Application of plant-derived bioactive substance DMPA in the control of adult tomato leafminer Technical Field

[0001] This invention belongs to the field of pest control, and in particular relates to the application of DMPA in the control of adult tomato leafminer moths. Background Technology

[0002] The tomato leafminer, an insect belonging to the genus *Solanum* in the family Lysimachiae, order Lepidoptera, is a devastating and invasive pest native to South America. Due to its wide temperature tolerance, diverse host crops, broad suitable habitat, high reproductive capacity, and significant damage, it has spread rapidly since invading my country, severely impacting the production of tomatoes and other solanaceous crops. The tomato leafminer primarily damages the plant in its larval stage. The larvae feed on the leaf tissue, particularly young leaves, creating translucent tunnels or spots of various shapes on the affected leaves. Later stages of infestation cause leaves to wrinkle and dry out, leaving only green veins. In addition, the larvae also bore into flower buds and fruits.

[0003] In 2023, the tomato leafminer was added to the "List of Class A Crop Diseases and Pests." Various control methods have been used to manage its occurrence, with synthetic chemical agents remaining the primary means of control. However, the widespread and extensive use of traditional chemical agents has led to increased resistance in the tomato leafminer. Therefore, finding new effective substances and developing novel insecticides is of great significance for the control of the tomato leafminer. Summary of the Invention

[0004] The technical problem to be solved by this invention is to provide a new method for the control of tomato leafminer.

[0005] The technical solution of this invention is: the application of 2,2-dimethyl-4-pentenoic acid in the control of adult tomato leafminer moths.

[0006] Furthermore, the method of prevention and control is to use 2,2-dimethyl-4-pentenoic acid for fumigation in the greenhouse.

[0007] Furthermore, the dosage of the 2,2-dimethyl-4-pentenoic acid is 1 × 10⁻⁶ per cubic meter of space. -4 More than 1 liter of 2,2-dimethyl-4-pentenoic acid.

[0008] Furthermore, the dosage of the 2,2-dimethyl-4-pentenoic acid is 2 × 10⁻⁶ per cubic meter of space. -4 More than 1 liter of 2,2-dimethyl-4-pentenoic acid.

[0009] Furthermore, the dosage of the 2,2-dimethyl-4-pentenoic acid is 4 × 10⁻⁶ per cubic meter of space. -4 2,2-Dimethyl-4-pentenoic acid (above 1 liter)

[0010] Furthermore, ethyl acetate is used as a solvent during the fumigation process.

[0011] Further, the volume ratio of 2,2-dimethyl-4-pentenoic acid to ethyl acetate is (0.5–2):(98–99.5). The use of 2,2-dimethyl-4-pentenoic acid in the preparation of drugs for controlling tomato leafminer.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] This invention has discovered that 2,2-dimethyl-4-pentenoic acid, a volatile gaseous component of *Nepenthes*, has an excellent control effect on adult tomato leafminer moths, with a concentration of 1×10⁻⁶. -4 A dosage of 2 × 10⁻⁶ liters per cubic meter is lethal. -4 At a dosage of 1 liter per cubic meter, the lethality rate reaches approximately 70%, 4 × 10 -4 With a dosage of approximately 100% per cubic meter, it can be used to control tomato leafminer in greenhouses. Attached Figure Description

[0014] Figure 1 shows the DMPA content detected in the odor volatiles from pitcher plant traps;

[0015] Figure 2 shows a scene from a fumigation experiment.

[0016] Figure 3 shows the mortality rate of DMPA fumigation on tomato leafminer moths;

[0017] Figure 4 shows the lethal phenotype of tomato leafminer caused by DMPA fumigation. Detailed Implementation

[0018] Unless otherwise specified, the experimental methods used in the following examples are conventional methods. Unless otherwise specified, the experimental materials used in the following examples were all purchased from commercial sources.

[0019] Example 1

[0020] A high level of 2,2-dimethyl-4-pentenoic acid (DMPA) was detected in the volatile aroma components of pitcher plants (Figure 1), which is one of the volatile aroma components of predatory pitcher plants. DMPA is a methyl-branched fatty acid with a molecular weight of 128.17 and can be used as an intermediate in organic synthesis. It has some solubility, is slightly soluble in water, and soluble in solvents such as alcohols and ethers. DMPA has good thermal and chemical stability and is not easily decomposed or deteriorated.

[0021] Example 2

[0022] To verify the control effect of DMPA on adult tomato leafminer moths, the following experiment was conducted.

[0023] (1) Experimental method: DMPA (≥98%) was prepared into 2%, 1%, and 0.5% (v / v) solutions using ≥99.5% ethyl acetate, respectively. The control was ≥99.5% ethyl acetate. 5 μL of the solution was dropped into a 1 cm solution. 2 The DMPA was applied to filter paper in three treatment groups (0.1 μL, 0.05 μL, and 0.025 μL). The filter paper was secured with a pin and suspended above the top of a 250 mL Erlenmeyer flask, which was then sealed with sealing film. Ten three-day-old adult tomato leafminers were tested for each treatment, with three replicates per treatment. The fumigation treatment lasted for 3 hours.

[0024] (2) Experimental results: 5 μL of ethyl acetate (≥99.5%) had no lethal effect on adult tomato leafminers. Different amounts of DMPA had varying degrees of lethal effect on tomato leafminers. 0.05 μL of DMPA had a good fumigation lethal effect on tomato leafminers (Figures 2, 3, and 4). This indicates that DMPA can be used for the control of tomato leafminers.

Claims

Application of 1,2,2-Dimethyl-4-pentenoic acid in the control of adult tomato leafminer moths.

2. The application according to claim 1, characterized in that, The method of prevention and control is to use 2,2-dimethyl-4-pentenoic acid for fumigation in greenhouses.

3. The application according to claim 2, characterized in that, The dosage of 2,2-dimethyl-4-pentenoic acid is 1×10⁻⁶ per cubic meter of space. -4 More than 1 liter of 2,2-dimethyl-4-pentenoic acid.

4. The application according to claim 3, characterized in that, The dosage of 2,2-dimethyl-4-pentenoic acid is 2 × 10⁻⁶ per cubic meter of space. -4 More than 1 liter of 2,2-dimethyl-4-pentenoic acid.

5. The application according to claim 4, characterized in that, The dosage of the 2,2-dimethyl-4-pentenoic acid is 4 × 10⁻⁶ per cubic meter of space. -4 More than 1 liter of 2,2-dimethyl-4-pentenoic acid.

6. The application according to any one of claims 2-5, characterized in that, Ethyl acetate was used as the solvent during the fumigation.

7. The application according to claim 6, characterized in that, The volume ratio of 2,2-dimethyl-4-pentenoic acid to ethyl acetate is (0.5-2):(98-99.5). 8.2,2-Dimethyl-4-pentenoic acid in the preparation of drugs for controlling tomato leafminer.

Images