Application of Artemisia dubia Wall. ex Bess. Extract in Promoting Growth of Crops, Increasing Yield of Crops or Preventing and Controlling Root Rot

Abstract

The present invention relates to the application of Artemisia dubia Wall. ex Bess. extract in promoting the growth of crops, increasing the yield of crops or preventing and controlling root rot. The present invention discovers that Artemisia dubia Wall. ex Bess. extract can promote the growth of crops, increase the yield of crops and prevent and control root rot. Preparing it into a seed treatment suspension and coating soybean seeds before sowing can effectively promote the growth of soybeans, increase the yield of soybeans and prevent and control soybean root rot.

Description

Technical Field

[0001] The present invention relates to the field of growth promoters or biocides, and particularly to the application of Artemisia dubia Wall. ex Bess. extract in promoting the growth of crops, increasing crop yields or controlling root rot diseases. Background Art

[0002] Soybeans are rich in high-quality protein and unsaturated fatty acids, and are common foods with relatively high nutritional value in daily life. Their planting area ranks fourth in the world, second only to rice, wheat, and corn. To increase supply, it is necessary to increase soybean yields. In addition, soybean root rot, as a soil-borne disease, is characterized by wide distribution, severe damage, multiple pathogen species, and difficult control. It seriously affects the yield and quality of soybeans, causing huge losses to soybean production. How to effectively control soybean root rot is of great significance for ensuring soybean production and quality safety. Summary of the Invention

[0003] One aspect of the present invention provides the application of Artemisia dubia Wall. ex Bess. extract in promoting the growth of crops, increasing crop yields or controlling root rot diseases. The Artemisia dubia Wall. ex Bess. extract is obtained by the following method: mixing the above-ground part of Artemisia dubia Wall. ex Bess. with 95% ethanol at a ratio of 1 g: (10 to 30) ml, and then performing ultrasonic extraction, wherein the ultrasonic frequency is 40 KHz, the ultrasonic power is 160 to 220 W, such as 180 W, the cyclic ultrasonic extraction time is 2 to 3 h, such as 2.5 h, the temperature is 30 to 50 °C, such as 40 °C. After natural cooling, suction filtration is carried out. After suction filtration, the filtrate is retained, and the filter residue is extracted more than 2 times. The filtrates obtained from several extractions are combined and concentrated to an extract paste to obtain the Artemisia dubia Wall. ex Bess. extract.

[0004] In a specific embodiment, the application is the application of Artemisia dubia Wall. ex Bess. extract in promoting the growth of soybeans or increasing soybean yields.

[0005] In a specific embodiment, the root rot disease is soybean root rot.

[0006] Another aspect of the present invention provides a composition, which comprises Artemisia dubia Wall. ex Bess. extract and a pharmaceutically acceptable carrier. The Artemisia dubia Wall. ex Bess. extract is obtained by the following method: mixing the above-ground part of Artemisia dubia Wall. ex Bess. with 95% ethanol at a ratio of 1 g: (10 to 30) ml, and then performing ultrasonic extraction, wherein the ultrasonic frequency is 40 KHz, the ultrasonic power is 160 to 220 W, such as 180 W, the cyclic ultrasonic extraction time is 2 to 3 h, such as 2.5 h, the temperature is 30 to 50 °C, such as 40 °C. After natural cooling, suction filtration is carried out. After suction filtration, the filtrate is retained, and the filter residue is extracted more than 2 times. The filtrates obtained from several extractions are combined and concentrated to an extract paste to obtain the Artemisia dubia Wall. ex Bess. extract.

[0007] In a specific embodiment, the pharmaceutically acceptable carrier includes at least one of a dispersion wetting agent, a film-forming agent, a thickening agent, a preservative, an antifreeze agent, and water.

[0008] In a specific embodiment, based on 100% of the total mass of the composition, the content of Artemisia dubia Wall. ex Bess. extract is 1% to 5%, the content of the dispersion wetting agent is 1.5% to 7%, the content of the film-forming agent is 3% to 7%, the content of the thickening agent is 0.2% to 6%, the content of the preservative is 0.1% to 1%, the content of the antifreeze agent is 3% to 5%, and the content of water is 49% to 71.2%.

[0009] In a specific embodiment, the dispersion wetting agent is Morwet EFW and / or Morwet IP.

[0010] In a specific embodiment, the film-forming agent is carboxymethyl cellulose.

[0011] In a specific embodiment, the thickening agent is xanthan gum and / or magnesium aluminum silicate.

[0012] In a specific embodiment, the preservative is sorbic acid.

[0013] In a specific embodiment, the antifreeze agent is ethylene glycol.

[0014] In a specific embodiment, based on 100% of the total mass of the composition, the content of Artemisia dubia Wall. ex Bess. extract is 1% to 5%, the content of Morwet EFW is 0.5% to 3%, the content of Morwet IP is 1% to 4%, the content of carboxymethyl cellulose is 3% to 7%, the content of xanthan gum is 0.1% to 3%, the content of magnesium aluminum silicate is 0.1% to 3%, the content of sorbic acid is 0.1% to 1%, the content of ethylene glycol is 3% to 5%, and the content of water is 49% to 71.2%.

[0015] In a specific embodiment, the composition is prepared by the following steps:

[0016] 1) Prepare a carboxymethyl cellulose solution;

[0017] 2) Prepare a xanthan gum solution;

[0018] 3) After fully dissolving the dispersion wetting agent in water, add Artemisia dubia Wall. ex Bess. extract, magnesium aluminum silicate, antifreeze agent, and preservative, and mix well to obtain a first material to be ground. Grind the first material to be ground at a temperature below 25 °C to obtain a first ground material;

[0019] 4) Mix the first grinding material, xanthan gum solution and carboxymethyl cellulose solution to form the second material to be ground. Grind the second material to be ground at a temperature below 25 °C for the second time. Stop the second grinding when 95% of the particles are less than 5 microns to obtain the seed treatment suspension.

[0020] In a specific embodiment, the first grinding is carried out with zirconia beads having a particle size of 1 to 1.2 mm.

[0021] In a specific embodiment, the second grinding is carried out with zirconia beads having a particle size of 0.8 to 1 mm.

[0022] In a specific embodiment, the first material to be ground is ground for the first time at 5 to 15 °C.

[0023] In a specific embodiment, the time for the first grinding is 2 to 5 hours.

[0024] In a specific embodiment, the second material to be ground is ground for the second time at 5 to 15 °C.

[0025] In a specific embodiment, the first grinding is carried out in a sand mill, and the zirconia beads with a particle size of 1 to 1.2 mm account for 60% to 75% of the volume of the grinding tank of the sand mill.

[0026] In a specific embodiment, the second grinding is carried out in a sand mill, and the zirconia beads with a particle size of 0.8 to 1 mm account for 60% to 75% of the volume of the grinding tank of the sand mill.

[0027] The third aspect of the present invention provides the use of the composition according to any one of the second aspects of the present invention in promoting the growth of crops, increasing the yield of crops or preventing and treating root rot.

[0028] Advantages of the present invention: The present invention discovers that the extract of Artemisia dubia Wall. ex Bess. can promote the growth of crops, increase the yield of crops and prevent and treat root rot. Description of the Drawings

[0029] Figure 1 Shows the results of various item indicators of the seed treatment suspension of Examples 2 to 5.

[0030] Figure 2 Shows the influence of the seed treatment suspension of Examples 2 to 4 on the growth of indoor soybeans.

[0031] Figure 3 Shows the prevention and treatment of field soybean root rot and the influence on soybean yield by the seed treatment suspension of Examples 2 to 4. Detailed Embodiments

[0032] The above content of the present invention will be further described in detail below in the form of preferred embodiments, but it does not limit the present invention.

[0033] Unless otherwise specified, the reagents in the embodiments of the present invention can be purchased through commercial channels.

[0034] Morwet EFW and Morwet IP were purchased from Nanjing Jieren Technology Co., Ltd.

[0035] Carboxymethyl cellulose (CMC) was purchased from Shandong Chengxin Chemical Technology Co., Ltd.

[0036] Xanthan gum was purchased from Guangdong Hengtian Biotechnology Co., Ltd.

[0037] Magnesium aluminum silicate was purchased from Wuhan Jiyesheng Chemical Co., Ltd.

[0038] Sorbic acid was purchased from Shanghai Macklin Biochemical Co., Ltd.

[0039] Ethylene glycol was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd.

[0040] Preparation of Artemisia dubia Wall. ex Bess. Extract Example 1

[0041] Take the aerial part of Artemisia dubia Wall. ex Bess., and use the ultrasonic extraction method. Crush Artemisia dubia Wall. ex Bess. with a pulverizer, mix the aerial part of Artemisia dubia Wall. ex Bess. with 95% ethanol at a ratio of 1 g: 30 ml, and perform ultrasonic extraction in an ultrasonic extractor. Among them, the ultrasonic frequency is 40 KHz, the ultrasonic power is 180 W, the cyclic ultrasonic extraction time is 2.5 h, the temperature is 40 °C. After natural cooling, perform suction filtration. After suction filtration, retain the filtrate, and soak the filter residue again with 95% ethanol at a ratio of 1 g: 30 ml. After repeating the extraction 3 times, combine the filtrates of several extractions, and concentrate them to an extract state under reduced pressure with a rotary evaporator to obtain the Artemisia dubia Wall. ex Bess. extract.

[0042] Using high performance liquid chromatography (HPLC), it was measured that the content of capillin in the Artemisia dubia Wall. ex Bess. extract was 31%.

[0043] Based on the determination of nitrogen, phosphorus and potassium in plants (molybdenum antimony anti-absorbance photometry of NY / T 2017-2011), the phosphorus element in the Artemisia dubia Wall. ex Bess. extract was detected, and the result was not detected.

[0044] Preparation of Seed Treatment Suspension Example 2

[0045] 1) Add 100 g of water to the batching tank. With the stirring device turned on, slowly and evenly sprinkle 50 g of carboxymethyl cellulose (CMC) into the batching tank and keep stirring. When the carboxymethyl cellulose (CMC) is evenly dispersed in water and there are no obvious large lumps, stop stirring to obtain carboxymethyl cellulose solution.

[0046] 2) Slowly add 10 g of xanthan gum to 100 g of water while stirring to obtain xanthan gum solution.

[0047] 3) Take 630 g of water and add it to a container. Then add 10 g of Morwet EFW and 20 g of Morwet IP and dissolve them fully. Then add 25 g of Artemisia dubia Wall. ex Bess. extract in paste form, 10 g of magnesium aluminum silicate, 40 g of ethylene glycol, and 5 g of sorbic acid and mix them evenly to obtain the first material to be ground. Then add the first material to be ground and zirconia beads with a particle size of 1 mm to a vertical sand mill and conduct the first grinding at 15 °C. Among them, the zirconia beads account for 70% of the tank volume, the rotation speed of the first grinding is 2000 revolutions per minute, and grind for 2 hours to obtain the first ground material.

[0048] 4) Mix the first ground material, xanthan gum solution, and carboxymethyl cellulose solution to form the second material to be ground. Add the second material to be ground and zirconia beads with a particle size of 0.8 mm to a vertical sand mill and conduct the second grinding at 15 °C. Among them, the zirconia beads account for 70% of the tank volume, the rotation speed of the second grinding is 2000 revolutions per minute. During this period, take out a small amount and use a laser particle size analyzer to detect the particle size of the particles. Stop the second grinding when 95% of the particles are less than 5 microns to prepare the seed treatment suspension. Example 3

[0049] 1) Add 100 g of water to the batching tank. With the stirring device turned on, slowly and evenly sprinkle 30 g of carboxymethyl cellulose (CMC) into the batching tank and keep stirring. When the carboxymethyl cellulose (CMC) is evenly dispersed in water and there are no obvious large lumps, stop stirring to obtain carboxymethyl cellulose solution.

[0050] 2) Slowly add 1 g of xanthan gum to 100 g of water while stirring to obtain xanthan gum solution.

[0051] 3) Add 712 g of water into a container, then add 5 g of Morwet EFW and 10 g of Morwet IP and dissolve them thoroughly. Then add 10 g of the extract paste of Artemisia dubia Wall., 1 g of magnesium aluminum silicate, 30 g of ethylene glycol, and 1 g of sorbic acid, and mix them evenly to obtain the first material to be ground. Then add the first material to be ground and zirconia beads with a particle size of 1.2 mm into a vertical sand mill and conduct the first grinding at 25 °C. Among them, the zirconia beads account for 60% of the tank volume, the rotation speed of the first grinding is 2000 revolutions per minute, and the first grinding lasts for 2 hours to obtain the first ground material.

[0052] 4) Mix the first ground material, xanthan gum solution and carboxymethyl cellulose solution to form the second material to be ground. Add the second material to be ground and zirconia beads with a particle size of 1.0 mm into a vertical sand mill and conduct the second grinding at 25 °C. Among them, the zirconia beads account for 60% of the tank volume, the rotation speed of the second grinding is 2000 revolutions per minute. During this period, take out a small amount and detect the particle size of the particles with a laser particle size analyzer. Stop the second grinding when 95% of the particles are less than 5 microns to prepare the seed treatment suspension. Example 4

[0053] 1) Add 100 g of water into a batching tank. With the stirring device turned on, slowly and evenly sprinkle 70 g of carboxymethyl cellulose (CMC) into the batching tank and keep stirring. When the carboxymethyl cellulose (CMC) is evenly dispersed in water and there are no obvious large lumps, stop stirring to obtain the carboxymethyl cellulose solution.

[0054] 2) Slowly add 30 g of xanthan gum to 100 g of water while stirring to obtain the xanthan gum solution.

[0055] 3) Take 490 g of water and add it to a container, then add 30 g of Morwet EFW and 40 g of Morwet IP and dissolve them thoroughly. Then add 50 g of the extract paste of Artemisia dubia Wall., 30 g of magnesium aluminum silicate, 50 g of ethylene glycol, and 10 g of sorbic acid, and mix them evenly to obtain the first material to be ground. Then add the first material to be ground and zirconia beads with a particle size of 1 mm into a vertical sand mill and conduct the first grinding at 5 °C. Among them, the zirconia beads account for 75% of the tank volume, the rotation speed of the first grinding is 2000 revolutions per minute, and the grinding lasts for 2 hours to obtain the first ground material.

[0056] 4) Mix the first grinding material, xanthan gum solution and carboxymethyl cellulose solution to form the second material to be ground. Add the second material to be ground and zirconia beads with a particle size of 0.8 mm into a vertical sand mill, and conduct the second grinding at 5 °C. Among them, the zirconia beads account for 75% of the volume of the tank body, and the rotation speed of the second grinding is 2000 revolutions per minute. During this period, take out a small amount for detection of the particle size of the particulate matter with a laser particle size analyzer, and stop the second grinding when 95% of the particles are less than 5 microns to prepare a seed treatment suspension. Example 5

[0057] 1) Add 100 grams of water to the batching tank. With the stirring device turned on, slowly and evenly sprinkle 50 grams of carboxymethyl cellulose (CMC) into the batching tank and keep stirring. When the carboxymethyl cellulose (CMC) is evenly dispersed in water and there are no obvious large lumps, stop stirring to obtain the carboxymethyl cellulose solution.

[0058] 2) Slowly add 2 grams of xanthan gum to 100 grams of water while stirring to obtain the xanthan gum solution.

[0059] 3) Take 603 grams of water and add it to a container, then add 10 grams of Morwet EFW and 20 grams of Morwet IP and dissolve them fully; then add 60 grams of the extract of Artemisia dubia Wall. ex Bess. in paste form, 10 grams of magnesium aluminum silicate, 40 grams of ethylene glycol, and 5 grams of sorbic acid, and mix them evenly to obtain the first material to be ground. Then add the first material to be ground and zirconia beads with a particle size of 1 mm into a vertical sand mill, and conduct the first grinding at 15 °C. Among them, the zirconia beads account for 70% of the volume of the tank body, and the rotation speed of the first grinding is 2000 revolutions per minute. Grind for 2 hours to obtain the first grinding material.

[0060] 4) Mix the first grinding material, xanthan gum solution and carboxymethyl cellulose solution to form the second material to be ground. Add the second material to be ground and zirconia beads with a particle size of 0.8 mm into a vertical sand mill, and conduct the second grinding at 15 °C. Among them, the zirconia beads account for 70% of the volume of the tank body, and the rotation speed of the second grinding is 2000 revolutions per minute. During this period, take out a small amount for detection of the particle size of the particulate matter with a laser particle size analyzer, and stop the second grinding when 95% of the particles are less than 5 microns to prepare a seed treatment suspension.

[0061] Determination of performance indicators of the seed treatment suspension

[0062] Analyze the control item indicators of the seed treatment suspensions in Examples 2 to 5, and the results are shown in Figure 1 .

[0063] From Figure 1It can be seen that the thermal storage stability of Example 5 does not meet the standard, and the test results of various indicators of the seed treatment suspension agents in Examples 2 to 4 all meet the standard, and the indicators such as suspension rate and pourability of Example 2 are better. Therefore, Examples 2 to 4 are selected for the next experiment.

[0064] Application Example 1: Influence on soybean growth indoors

[0065] The tested soybean variety is Henong 75.

[0066] The experiment was carried out in a greenhouse. The greenhouse environmental conditions were a room temperature of 23 °C, a relative humidity of 65%, LED light source for supplementary lighting, and 12 h of alternating light and darkness.

[0067] The soybean seeds were soaked in an aqueous ethanol solution of 75 wt% for 30 s and then poured out, and rinsed 3 times with sterile water; then soaked in an aqueous sodium hypochlorite solution of 1 wt% for 1.5 min, and continuously stirred during this period to make the seeds fully blend with the aqueous sodium hypochlorite solution, and then repeatedly rinsed 3 times with sterile water to obtain pretreated soybean seeds. The seed treatment suspension agents in Examples 2 to 4 and the pretreated soybean seeds were mixed according to a mass ratio of 1:100, and then dried to obtain coated soybean seeds. The coated soybeans were sown in flower pots containing sterilized nutrient soil. Among them, the sterilized nutrient soil was prepared by mixing sterile soil and sterilized vermiculite according to a volume ratio of 1:1. The flower pot specifications were 11 cm in diameter and 9 cm in height, with 2 soybean seeds per pot, 12 pots for each treatment, and repeated 4 times. The pretreated soybean seeds without coating were used as the blank control. After 21 d, 7 plants were selected from each repetition of each treatment to measure four indicators of the soybean plants: plant height, root length, aboveground fresh weight, and underground fresh weight, and then the promotion rate of each indicator was calculated based on the following indicators.

[0068] Index promotion rate = (index value of the coating treatment group - index value of the blank control group) × 100% / index value of the blank control group.

[0069] Then, data statistics were carried out using Excel 2021 software, the experimental data were statistically analyzed using SPSS 25.0 software, and the Duncan's new multiple range method was used for significance test of differences. The results are shown in Figure 2 .

[0070] From Figure 2It can be seen that the emergence rates of Examples 2 to 4 are all above 90%, and the emergence rates are relatively good; compared with the blank control group, the promoting effect of Example 2 on the plant height of soybean plants reaches 19%, the promoting effect on the root length of soybean plants reaches 36%, the promoting effect on the fresh weight of the above-ground part reaches 40%, and the promoting effect on the fresh weight of the underground part reaches 60%; the promoting effect of Example 3 on the plant height of soybean plants reaches 14%, the promoting effect on the root length of soybean plants reaches 21%, the promoting effect on the fresh weight of the above-ground part reaches 29%, and the promoting effect on the fresh weight of the underground part reaches 46%; the promoting effect of Example 4 on the plant height of soybean plants reaches 14%, the promoting effect on the root length of soybean plants reaches 22%, the promoting effect on the fresh weight of the above-ground part reaches 32%, and the promoting effect on the fresh weight of the underground part reaches 51%. It shows that the Artemisia dubia Wall. ex Bess. extract seed treatment suspension agent of the present invention has a promoting effect on the emergence rate, plant height, root length and fresh weight of soybeans, and Example 2 has the best promoting effect on soybean growth.

[0071] Application Example 2: Control of soybean root rot in the field and its effect on soybean yield

[0072] The experiment referred to GB / T17980.88-2004 "Pesticide field efficacy test guidelines (part 2) - Part 88: Fungicides for controlling soybean root rot".

[0073] The experiment was carried out in Yonghe Village, Yongfeng Town, Qinggang County, Suihua City, Heilongjiang Province. The previous crop was soybean, and root rot occurred relatively seriously in previous years. The cultivation conditions of all experimental plots were the same and met the local scientific agricultural practice (GAP). A randomized block design was adopted, with a plot area of 20m 2 , and 1 plot was used as one replication, with 4 replications for each treatment.

[0074] Control object: Soybean root rot.

[0075] Test variety: The soybean variety was Henong 75.

[0076] The seed treatment suspension agents of Examples 2 to 4 and soybean seeds were mixed at a mass ratio of 1:100 to coat the soybean seeds, and then dried to obtain the coated soybean seeds, which were sown on May 14, 2024. The uncoated soybean seeds were used as the blank control.

[0077] Investigation of soybean root rot: The incidence of soybean root rot was investigated once at 10d, 30d, and 60d after soybean emergence. During the investigation, the 5-point sampling method was used for sampling. 10 soybean plants were randomly dug at each point, and the control effect was calculated based on the disease index by grading according to the disease conditions of the roots and the base of the stems.

[0078] Grading method: Grade 0: There are no lesions at the base of the stem and the main fibrous roots of the plant; Grade 1: There are a few lesions at the base of the stem and the main root of the plant; Grade 3: There are many lesions at the base of the stem or the main root of the plant, and the area of ​​lesions accounts for 1 / 4~1 / 2 of the total area of ​​the stem and roots; Grade 5: There are many and large lesions at the base of the stem and the main root of the plant, and the area of ​​lesions accounts for 1 / 2~3 / 4 of the total area of ​​the base of the stem and the root;

[0079] Level 7: The lesions on the base of the plant stem and the main root are connected, forming a stem-wrapped phenomenon, but the root system is not dead; Level 9: The root system of the plant is necrotic, and the above-ground part of the plant wilts or dies.

[0080] The calculation of disease index and control effect is carried out in accordance with GB / T17980.88-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 88: Fungicide Control of Soybean Root Rot".

[0081] Investigation of soybean yield components: At harvest, 10 healthy representative plants were selected from each treatment, including the blank control, and the number of nodes, number of pods and 100-grain weight were investigated.

[0082] Crop safety observation: While conducting the soybean root rot investigation, observe whether the pesticide has any damage to the crop. If there is any damage, record the type and degree of damage. Also observe whether there is any impact on other organisms.

[0083] Excel 2021 software was used to perform statistical or calculation of disease index, control effect, number of nodes per plant, number of pods per plant, 100-grain weight and 100-grain weight growth. SPSS 25.0 software was used to perform statistical analysis on the test data. Duncan's new multiple range method was used for significant difference test. The results are shown in Figure 3 .

[0084] Figure 3 The results showed that after using the seed treatment suspensions of Examples 2 to 4, the disease index at 10d, 30d, and 60d after emergence was reduced compared with the blank control. 10d after emergence, the control effect of Example 2 on soybean root rot was 89.19%, the control effect of Example 3 on soybean root rot was 81.47%, and the control effect of Example 4 on soybean root rot was 84.55%; 30d after emergence, the control effect of Example 2 on soybean root rot was 93.41%, the control effect of Example 3 on soybean root rot was 81.32%, and the control effect of Example 4 on soybean root rot was 86.26%; 60d after emergence, the control effect of Example 2 on soybean root rot was 88.32%, the control effect of Example 3 on soybean root rot was 78.48%, and the control effect of Example 4 on soybean root rot was 83.20%. From the perspective of yield components, Examples 2 and 4 were significantly higher than the blank control in terms of plant node number, plant pod number, and 100-grain weight. In summary, coating soybean seeds with Artemisia selengensis extract seed treatment suspension has a good preventive and therapeutic effect on soybean root rot and has a significant yield-increasing effect.

[0085] Crop safety observation results: No obvious phytotoxicity was found on soybeans in each treatment during the entire crop growth period, and there was no impact on other organisms.

[0086] As can be seen from the above examples and application examples, the Artemisia dubia Wall. extract seed treatment suspension provided by the present invention can not only improve the emergence rate, promote the growth of plant height, root length and fresh weight of soybean plants, but also has a good control effect and yield increase effect on soybean root rot.

Claims

1. Application of Artemisia selengensis extract in promoting soybean growth or increasing soybean yield, wherein the Artemisia selengensis extract is extracted by the following method: mixing the aerial part of Artemisia selengensis with 95% ethanol at a ratio of 1 g: (10 to 30) ml, and then performing ultrasonic extraction, wherein: The ultrasonic frequency is 40KHz, the ultrasonic power is 160 to 220W, the cyclic ultrasonic extraction time is 2 to 3h, the temperature is 30 to 50 degrees Celsius, and the extract is filtered after natural cooling. The filtrate is retained after filtration, and the residue is repeatedly extracted for more than 2 times. The filtrates from several extractions are combined and concentrated to an extract to obtain the Artemisia oxtail extract.

2. A composition comprising an Artemisia oxysporum extract and an insecticide acceptable carrier, wherein the Artemisia oxysporum extract is extracted by the following method: mixing the aerial part of Artemisia oxysporum with 95% ethanol at a ratio of 1 g: (10 to 30) ml, and then performing ultrasonic extraction, wherein: The ultrasonic frequency is 40KHz, the ultrasonic power is 160 to 220W, the cyclic ultrasonic extraction time is 2 to 3h, the temperature is 30 to 50 degrees Celsius, and the extract is filtered after natural cooling. The filtrate is retained after filtration, and the residue is repeatedly extracted for more than 2 times. The filtrates from several extractions are combined and concentrated to an extract to obtain the Artemisia oxtail extract.

3. The composition according to claim 2, characterized in that The pesticide-acceptable carrier comprises at least one of a dispersing wetting agent, a film-forming agent, a thickening agent, a preservative, an antifreeze agent and water.

4. The composition according to claim 3, characterized in that Based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 1%, the content of the dispersing wetting agent is 1.5%, the content of the film-forming agent is 3%, the content of the thickener is 0.2%, the content of the preservative is 0.1%, the content of the antifreeze agent is 3%, and the content of water is 91.2%; or Based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 2.5%, the content of the dispersing wetting agent is 3%, the content of the film-forming agent is 5%, the content of the thickener is 2%, the content of the preservative is 0.5%, the content of the antifreeze agent is 4%, and the content of water is 83%; or Taking the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 5%, the content of the dispersing wetting agent is 7%, the content of the film-forming agent is 7%, the content of the thickener is 6%, the content of the preservative is 1%, the content of the antifreeze agent is 5%, and the content of water is 69%.

5. The composition according to claim 3, characterized in that The dispersing wetting agent is Morwet EFW and / or Morwet IP; and / or The film-forming agent is carboxymethyl cellulose; and / or The thickener is xanthan gum and / or magnesium aluminum silicate; and / or The preservative is sorbic acid; and / or The antifreeze agent is ethylene glycol.

6. The composition according to claim 5, characterized in that Based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 1%, the content of Morwet EFW is 0.5%, the content of Morwet IP is 1%, the content of carboxymethyl cellulose is 3%, the content of xanthan gum is 0.1%, the content of magnesium aluminum silicate is 0.1%, the content of sorbic acid is 0.1%, the content of ethylene glycol is 3%, and the content of water is 91.2%; or Based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 2.5%, the content of Morwet EFW is 1%, the content of Morwet IP is 2%, the content of carboxymethyl cellulose is 5%, the content of xanthan gum is 1%, the content of magnesium aluminum silicate is 1%, the content of sorbic acid is 0.5%, the content of ethylene glycol is 4%, and the content of water is 83%; or Based on the total mass of the composition as 100%, the content of the Artemisia oxtail extract is 5%, the content of the Morwet EFW is 3%, the content of the Morwet IP is 4%, the content of the carboxymethyl cellulose is 7%, the content of the xanthan gum is 3%, the content of the magnesium aluminum silicate is 3%, the content of the sorbic acid is 1%, the content of the ethylene glycol is 5%, and the content of the water is 69%.

7. The composition according to claim 5 or 6, characterized in that The composition is prepared by the following steps: 1) Prepare carboxymethyl cellulose solution; 2) Prepare xanthan gum solution; 3) After the dispersing wetting agent is fully dissolved in water, the Artemisia oxtail extract, magnesium aluminum silicate, antifreeze agent and preservative are added, and the mixture is fully mixed to obtain a first material to be ground, and the first material to be ground is first ground at a temperature below 25 degrees Celsius to obtain a first ground material; 4) The first grinding material, xanthan gum solution and carboxymethyl cellulose solution are mixed to form a second grinding material, and the second grinding is performed on the second grinding material at a temperature below 25 degrees Celsius until 95% of the particles are smaller than 5 microns, thereby obtaining a seed treatment suspension.

8. Use of the composition according to any one of claims 2 to 7 in promoting soybean growth or increasing soybean yield.

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