Application of Nepeta extract in regulating the growth of crops

Abstract

The present invention belongs to the technical fields of natural product extraction and medicinal plants, and particularly relates to the application of Schizonepeta tenuifolia extract in regulating the growth of crops. The Schizonepeta tenuifolia extract includes Schizonepeta tenuifolia inflorescence extract and / or Schizonepeta tenuifolia stem extract; the Schizonepeta tenuifolia inflorescence extract includes Schizonepeta tenuifolia inflorescence water extract, Schizonepeta tenuifolia inflorescence water extract, and Schizonepeta tenuifolia inflorescence ethanol extract; the Schizonepeta tenuifolia stem extract includes Schizonepeta tenuifolia stem water extract, Schizonepeta tenuifolia stem water extract, and Schizonepeta tenuifolia stem ethanol extract. The Schizonepeta tenuifolia extract provided by the present invention has a significant inhibitory effect on the germination of lettuce seeds and the growth of seedlings, while having a significant promoting effect on the germination of barley and green vegetable seeds and the growth of seedlings. Among vegetables and crops, the use of Schizonepeta tenuifolia extract to inhibit weeds around the required vegetables and crops will also promote the growth of the plants, and can replace the use of chemical reagents while avoiding the adverse effects of chemical reagents on the environment and the quality of fruits and vegetables.

Description

Technical Field

[0001] The present invention belongs to the technical fields of natural product extraction and medicinal plants, and particularly relates to the application of Nepeta cataria extract in regulating the growth of crops. Background Art

[0002] Biodiversity refers to the diversity and variability of life on Earth and plays a crucial role in ecological functions. The comprehensive utilization of plant biodiversity is one of the ways to improve food security and sustainable agriculture. Species combinations such as multiple cropping, intercropping, crop rotation, and mulching also have a positive impact on crop productivity and yield stability. The interaction between plant species may include the production and release of bioactive substances, which directly or indirectly affect the growth and development of other organisms, and this phenomenon is called allelopathy. This definition was later revised to refer to any process of secondary metabolites produced by plants, microorganisms, viruses, and fungi that affect the growth and development of agricultural and biological systems, including positive and negative effects. The secondary metabolites related to allelopathy are released into the environment through volatilization, leaching, root exudation, and the decomposition of plant residues in the soil, and are called allelochemicals. These allelochemicals exist in different parts of various plants, such as leaves, roots, rhizomes, stems, flowers, pollen, and fruits, in natural ecological processes. Some plants are not dominant competitors in their natural habitats, but show strong succession advantages when introduced into new areas. Allelopathy is generally considered an important ecological factor determining the structure and composition of plant communities.

[0003] Despite recent progress in the development of agricultural chemicals for controlling pests and diseases in modern agriculture, there are still diseases. This is mainly due to pests, pathogens, and weeds. Weeds are particularly destructive, and more than 240 weed species have been found to have allelopathic effects on surrounding plants, whether on the same species or on other crop and weed species. However, scientists in many different habitats around the world have demonstrated the necessity of agricultural chemical pest control. The allelopathy of many plant species has been reported. For example, among 245 plant species in the Sino-Japanese flora, 84 species have significant inhibitory activities, and among them, 10 species show the strongest effects. The evaluation of the allelopathic potential of 83 Iranian medicinal plants found that Peganum harmala , Berberis vulgaris , Artemisia aucheri and Ferulago angulateThe inhibitory effect on the root growth of lettuce exceeds 80%. The allelopathic potential assessment of medicinal plant species used in Ghana found that 75 out of 183 medicinal plants significantly inhibited the radicle growth of lettuce through leaf leachates. Especially considering the increasing popularity of organic products in the past decade. The secondary metabolites of medicinal plants are considered to have strong allelopathic effects. In addition, it is easier to analyze medicinal plants to discover new natural compounds than other plants. Some bioactive substances in medicinal plants, such as ferulic acid, coumaric acid, vanillic acid, caffeic acid, and chlorogenic acid, have been found to have inhibitory effects on plant growth. Therefore, new strategies for developing the allelopathic potential of medicinal plants in regulating plant growth and development are needed. Summary of the Invention

[0004] The object of the present invention is to develop new strategies for the allelopathic potential of medicinal plants in regulating plant growth and development, expand the application of medicinal plants in more fields, explore the allelopathic effects of different parts of Nepeta cataria under laboratory conditions using the sandwich method and plate assay method, and provide the application of Nepeta cataria extract in regulating the growth of crops. To achieve the above object, the present invention adopts the following technical solutions.

[0005] The present invention provides an application of Nepeta cataria extract in regulating the growth of crops, wherein the Nepeta cataria extract regulates the growth of the crops through allelopathic effects on the crops; the Nepeta cataria extract includes Nepeta cataria inflorescence extract and / or Nepeta cataria stem extract.

[0006] The Nepeta cataria inflorescence extract includes at least one of Nepeta cataria inflorescence water extract, Nepeta cataria inflorescence water extract, and Nepeta cataria inflorescence ethanol extract.

[0007] The Nepeta cataria stem extract includes at least one of Nepeta cataria stem water extract, Nepeta cataria stem water extract, and Nepeta cataria stem ethanol extract.

[0008] The Nepeta cataria inflorescence water extract, the Nepeta cataria inflorescence water extract, and the Nepeta cataria inflorescence ethanol extract are all obtained by using different methods with Nepeta cataria inflorescence as the material.

[0009] The Nepeta cataria stem water extract, the Nepeta cataria stem water extract, and the Nepeta cataria stem ethanol extract are all obtained by using different methods with Nepeta cataria stem as the material.

[0010] In order to develop new strategies for the allelopathic potential of medicinal plants in regulating plant growth and development, the present invention provides an application of Nepeta cataria extract in regulating the growth of crops, and expands the application of Nepeta cataria extract in more fields. The research found that the Nepeta cataria extract provided by the present invention can be used to regulate the growth of crops, and thus play a role in regulating the germination of crop seeds and / or the growth of seedlings.

[0011] Preferably, the crops include any one or more of lettuce, barley, and green vegetables. Taking these three crops, lettuce, barley, and green vegetables, as representative plants for allelopathy research receptor plants can facilitate exploring the effects of allelochemicals on the growth of crop plants under different environmental stresses, as well as how the stress resistance mechanisms of crop plants interact with allelopathy, providing ideas for cultivating crop varieties with strong stress resistance.

[0012] Preferably, the Nepeta extract is used for regulating the seed germination and seedling growth of the crops.

[0013] Preferably, the regulation includes promoting and / or inhibiting the seed germination and seedling growth of the crops.

[0014] Preferably, the preparation method of the aqueous extract of Nepeta cataria inflorescence includes the following steps:

[0015] After drying the Nepeta cataria inflorescence, at 4°C, add water and extract for 48 hours, then filter, and collect the filtrate to obtain the aqueous extract of Nepeta cataria inflorescence.

[0016] The preparation method of the aqueous extract of Nepeta cataria stem includes the following steps:

[0017] After drying the Nepeta cataria stem, at 4°C, add water and extract for 48 hours, then filter, and collect the filtrate to obtain the aqueous extract of Nepeta cataria stem.

[0018] The material ratio of the Nepeta cataria inflorescence to water and the material ratio of the Nepeta cataria stem to water are both 8g:100mL. This material ratio can also ensure the concentration and purity of the extract, improve the extraction efficiency and economy. Specifically, the material ratio of the Nepeta cataria inflorescence to water and the material ratio of the Nepeta cataria stem to water are both 8g:100mL. This optimized material ratio can ensure the concentration and purity of the extract. An appropriate material ratio can not only ensure sufficient contact between the solvent and the plant material but also avoid the dilution effect caused by excessive solvent, thus maintaining the concentration of the active ingredients in the extract during the extraction process and improving the activity of the extract.

[0019] At the same time, the above material ratio can improve the extraction efficiency, reduce the amount of solvent used, and lower the production cost. At the same time, by precisely controlling the material ratio, the consistency of each extraction process can be ensured, improving the stability and repeatability of the product, which is of great significance for large-scale production and application.

[0020] The preparation methods of the aqueous extract of Nepeta cataria inflorescence and the aqueous extract of Nepeta cataria stem provided by the present invention can more effectively extract the active ingredients in Nepeta cataria through low-temperature water extraction and the above material ratio, enhancing their effects in anti-inflammatory, antiviral, antioxidant, and immunomodulatory aspects, thus effectively solving the related problems existing in the prior art.

[0021] Preferably, the preparation method of the Nepeta cataria L. inflorescence water extract comprises the following steps:

[0022] After drying the Nepeta cataria L. inflorescence, using water as the first extraction solvent, perform the first extraction, and collect the extract to obtain the Nepeta cataria L. inflorescence water extract.

[0023] The preparation method of the Nepeta cataria L. stem water extract comprises the following steps:

[0024] After drying the Nepeta cataria L. stem, using water as the first extraction solvent, perform the first extraction, and collect the extract to obtain the Nepeta cataria L. stem water extract.

[0025] The material ratio of the Nepeta cataria L. inflorescence to the first extraction solvent is 5 g: 3 mL; the material ratio of the Nepeta cataria L. stem to the first extraction solvent is 10 g: 6 mL.

[0026] Preferably, the conditions for the first extraction are: extraction temperature 100 °C, extraction pressure 10.34 Mpa, extraction time 4 min.

[0027] Preferably, the preparation method of the Nepeta cataria L. inflorescence ethanol extract comprises the following steps:

[0028] After drying the Nepeta cataria L. inflorescence, using 75% ethanol by volume as the second extraction solvent, perform the second extraction, and collect the extract to obtain the Nepeta cataria L. inflorescence ethanol extract.

[0029] The preparation method of the Nepeta cataria L. stem ethanol extract comprises the following steps:

[0030] After drying the Nepeta cataria L. stem, using 75% ethanol by volume as the second extraction solvent, perform the second extraction, and collect the extract to obtain the Nepeta cataria L. stem ethanol extract.

[0031] The material ratio of the Nepeta cataria L. inflorescence to the second extraction solvent is 8 g: 4.8 mL; the material ratio of the Nepeta cataria L. stem to the second extraction solvent is 10 g: 6 mL.

[0032] Preferably, the conditions for the second extraction are: extraction temperature 100 °C, extraction pressure 10.34 Mpa, extraction time 5 min.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] 1. The purpose of the present invention is to develop new strategies for allelopathic potential of medicinal plants in regulating plant growth and development, to provide an application of Nepeta cataria extract in regulating the growth of crops, and to expand the application of medicinal plants in more fields. Through the study of the allelopathy of Nepeta cataria, it is found that the water extracts of Nepeta cataria inflorescence, Nepeta cataria stem, the water extracts of Nepeta cataria inflorescence, Nepeta cataria stem, the ethanol extracts of Nepeta cataria inflorescence and Nepeta cataria stem have significant inhibitory effects on the germination of lettuce seeds and the growth of seedlings, while they have significant promoting effects on the germination of barley and green vegetable seeds and the growth of seedlings. The Nepeta cataria extract provided by the present invention can be used to regulate the growth of crops, and thus play a role in regulating the germination of crop seeds and / or the growth of seedlings.

[0035] 2. Through the study of the allelopathy of Nepeta cataria, it is found that the above-ground part of Nepeta cataria, the water extracts of Nepeta cataria inflorescence, Nepeta cataria stem, the water extracts of Nepeta cataria inflorescence, Nepeta cataria stem, the ethanol extracts of Nepeta cataria inflorescence and Nepeta cataria stem have significant inhibitory effects on the germination of lettuce seeds and the growth of seedlings, while they have significant promoting effects on the germination of barley and green vegetable seeds and the growth of seedlings. Moreover, the volatile substances in Nepeta cataria stem have a stronger inhibitory effect on lettuce than Nepeta cataria inflorescence, indicating that the content of inhibitory volatile substances is higher in Nepeta cataria stem. On the contrary, the volatile substances in Nepeta cataria inflorescence have a stronger promoting effect on barley and green vegetables than Nepeta cataria stem, indicating that the content of promoting volatile substances is higher in the inflorescence.

[0036] 3. The inhibitory effect of the water extract of Nepeta cataria inflorescence on lettuce is stronger than that of the water extract of Nepeta cataria stem, indicating that the content of inhibitory volatile substances is higher in Nepeta cataria inflorescence. Similarly, the promoting effect of the water extract of Nepeta cataria inflorescence on barley and green vegetables is stronger than that of the water extract of Nepeta cataria stem, indicating that the content of promoting volatile substances is higher in Nepeta cataria inflorescence.

[0037] The inhibitory effect of the water extract and ethanol extract of Nepeta cataria stem on lettuce is stronger than that of the water extract and ethanol extract of Nepeta cataria inflorescence, and the promoting effect on barley and green vegetables is also stronger than that of Nepeta cataria stem, indicating that the content of inhibitory volatile substances and promoting volatile substances is higher in Nepeta cataria stem.

[0038] Therefore, among vegetables and crops, using an appropriate concentration of the water extract, water extract or ethanol extract of Nepeta cataria to inhibit the weeds around the required vegetables and crops while also promoting the growth of the plants can replace the use of chemical reagents and avoid the adverse effects of chemical reagents on the environment and the quality of fruits and vegetables.

[0039] The experimental results show that Nepeta cataria has strong allelopathic potential against weeds. Once there are more Nepeta cataria plants in the environmental protection area with a large variety of plant species, there is a chance of causing relatively serious harm. For example, the growth of plants in this protection area will be affected and they cannot grow healthily or even die directly, resulting in a decrease in the number of plant species and an imbalance in the ecological environment. Therefore, Nepeta cataria should be discovered and cleared in a timely manner during management and maintenance. In addition, when it is necessary to remove certain weeds in agriculture, the extract of Nepeta cataria or plant residues can be appropriately utilized, which may have a certain effect on removal. Description of the Drawings

[0040] Figure 1 This shows the effect of the Nepeta cataria inflorescence by the sandwich method on the growth rate of the seeds of receptor plants; among them, "R" represents the radicle, and "H" represents the hypocotyl.

[0041] Figure 2 This shows the effect of the Nepeta cataria stem by the sandwich method on the growth rate of the seeds of receptor plants; among them, "R" represents the radicle, and "H" represents the hypocotyl.

[0042] Figure 3 This shows the effect of the water extract of Nepeta cataria inflorescence at different concentrations on the growth of lettuce seedlings, expressed by the growth rate, and its unit is %; among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0043] Figure 4 This shows the effect of the water extract of Nepeta cataria stem at different concentrations on the growth of lettuce seedlings, expressed by the growth rate, and its unit is %; among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0044] Figure 5 This shows the effect of the water extract of the above-ground part of Nepeta cataria at different concentrations on the growth of lettuce seedlings, expressed by the growth rate, and its unit is %; among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0045] Figure 6 This shows the comparison of the effects of the water extracts of different parts of Nepeta cataria on the radicle growth rate of lettuce seedlings at different concentrations; among them, the water extracts of different parts of Nepeta cataria include the water extract of Nepeta cataria stem, the water extract of Nepeta cataria inflorescence, and the water extract of the above-ground part of Nepeta cataria.

[0046] Figure 7 This shows the effect of the water extract of Nepeta cataria inflorescence at different concentrations on the growth of barley seedlings, expressed by the growth rate, and its unit is %; among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0047] Figure 8 This shows the effect of the water extract of Nepeta cataria stem at different concentrations on the growth of barley seedlings, expressed by the growth rate, and its unit is %; among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0048] Figure 9 The effects of the water extract of Nepeta cataria L. inflorescence at different concentrations on the growth of Brassica chinensis L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0049] Figure 10 The effects of the water extract of Nepeta cataria L. stem at different concentrations on the growth of Brassica chinensis L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0050] Figure 11 The effects of the ethanol extract of Nepeta cataria L. inflorescence at different concentrations on the growth of Lactuca sativa L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0051] Figure 12 The effects of the ethanol extract of Nepeta cataria L. stem at different concentrations on the growth of Lactuca sativa L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0052] Figure 13 The effects of the ethanol extract of Nepeta cataria L. inflorescence at different concentrations on the growth of Hordeum vulgare L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0053] Figure 14 The effects of the ethanol extract of Nepeta cataria L. stem at different concentrations on the growth of Hordeum vulgare L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0054] Figure 15 The effects of the ethanol extract of Nepeta cataria L. inflorescence at different concentrations on the growth of Brassica chinensis L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0055] Figure 16 The effects of the ethanol extract of Nepeta cataria L. stem at different concentrations on the growth of Brassica chinensis L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0056] Figure 17 The effects of the water extract of Nepeta cataria L. inflorescence at different concentrations on the growth of Lactuca sativa L. seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0057] Figure 18The effects of the water extract of Schizonepeta tenuifolia Briq. stems at different concentrations on the growth of lettuce seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0058] Figure 19 The effects of the water extract of Schizonepeta tenuifolia Briq. inflorescences at different concentrations on the growth of barley seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0059] Figure 20 The effects of the water extract of Schizonepeta tenuifolia Briq. stems at different concentrations on the growth of barley seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0060] Figure 21 The effects of the water extract of Schizonepeta tenuifolia Briq. inflorescences at different concentrations on the growth of pakchoi seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0061] Figure 22 The effects of the water extract of Schizonepeta tenuifolia Briq. stems at different concentrations on the growth of pakchoi seedlings are expressed by the growth rate, with the unit of %. Among them, "R" represents the radicle, and "H" represents the hypocotyl respectively. Detailed implementation manners

[0062] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments, but it should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following embodiments are conventional means well known to those skilled in the art. The materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0063] The Schizonepeta tenuifolia Briq. samples of the present invention are sampled through two ways: natural collection and purchase. The first time was in July 2022, collected from the Hanshan Nature Reserve in Jarud Banner, Tongliao City. The second time was purchased from Inner Mongolia Muxin Pharmaceutical Co., Ltd.

[0064] Receptor plants: lettuce, barley and pakchoi.

[0065] Among them, the lettuce ( Lactuca sativa var. ramosa Hort .) seeds were purchased from Hebei Qingfeng Seed Industry Co., Ltd., the barley ( Hordeum vulgare L.) seeds were purchased from Henan Liaodong Granary, and the pakchoi was Shanghaiqing ( Brassica rapa var . chinensis (L.) Kitam.) seeds were purchased from Qingxian Xingyun Seed Industry Co., Ltd.

[0066] The collected Schizonepeta tenuifolia Briq. samples were processed as follows:

[0067] First, dry at 60°C in a drying oven for 4 hours. Then, store in a sealed specimen bag in a dark place.

[0068] Example 1

[0069] I. Method

[0070] 1. Sandwich method

[0071] The sandwich method was developed based on the action of active substances from tree leaves and plant residues as an assay. In the preliminary research of the present invention, it was known that the average annual leaf fall of general trees was calculated as 3t / 10000m

[0072] According to the calculation of the preliminary research, it varies according to different plant growth environments and regions and is used as a standard with 3t. The collected Nepeta samples were dried in a 60°C drying oven for 4 hours respectively. Among them, the Nepeta samples were Nepeta stems and Nepeta inflorescences. 2 According to the calculation of the preliminary research, it varies according to different plant growth environments and regions and is used as a standard with 3t. After the collected Nepeta samples, which were Nepeta stems and Nepeta inflorescences, were dried in a 60°C drying oven for 4 hours.

[0073] Mix nutrient agar with distilled water at a ratio of 15mg:2mL, that is, 2mL of distilled water and 15mg of nutrient agar, weigh and prepare to obtain a nutrient agar medium. Put the nutrient agar medium into an autoclave and sterilize at 115°C for 15 minutes. Use a 6-well culture dish. Weigh 10mg and 50mg of the processed Nepeta samples respectively. 10mg for 3 wells and 50mg for 3 wells, and put them into the 6-well culture dish. Put 5mL of the above nutrient agar into each well. After waiting for the nutrient agar to solidify, put another 5mL of nutrient agar. After the nutrient agar solidifies, plant the seeds of 3 receptor plants, 5 seeds in each well. In total: 6 wells × 5 seeds = 30 seeds. Embed in the nutrient agar medium in the form of a sandwich. At the same time, set up a 6-well culture dish without receptor plants under the same conditions as a control group for the experiment. Let the 3 receptor plants germinate and grow. After sealing the six-well culture dish and wrapping it with tin foil, put it into a 25°C constant temperature incubator and culture for 3 days, then measure the radicle length and hypocotyl length of the seeds of the 3 receptor plants, and estimate the biological activity of the 3 receptor plants.

[0074] Among them, the processed Nepeta samples are the dried Nepeta stems and the dried Nepeta inflorescences.

[0075] 6-well culture dish: Multidish 6 Wells, NunclonDelta Si, Thermo Fisher Scientific, China.

[0076] Three receptor plants: lettuce, barley, and green vegetables.

[0077] The English name for radicle length is Radicle. The English name for hypocotyl length is Hypocotyl.

[0078] It should be noted that the reason for using three receptor plants as donor animals and plants in the present invention is as follows: Lettuce has high sensitivity and good reproducibility, representing dicotyledonous weeds. Among the chemical substances produced by plants, the parts where inhibitory activity is generated vary depending on the substance. For example, some substances inhibit the radicles of the seedlings of receptor plants, and some substances inhibit the growth of hypocotyls. In order to master the inhibitory activity and to accurately confirm both aspects of verification, the lengths of the radicles and hypocotyls of the seedlings were measured.

[0079] 2. Preparation of the extract

[0080] (1) After drying the collected Schizonepeta stems and Schizonepeta inflorescences separately in an oven, the dried Schizonepeta stems and dried Schizonepeta inflorescences were mixed at a mass ratio of 1:1 to obtain a mixture of Schizonepeta stems and Schizonepeta inflorescences. The mixture of Schizonepeta stems and Schizonepeta inflorescences is called the above-ground part of Schizonepeta.

[0081] The above-ground part of Schizonepeta was crushed, passed through a 20-mesh sieve, and distilled water was added for extraction at a mass-to-volume ratio of 8 g / 100 mL of the above-ground part of Schizonepeta. Static extraction was carried out at 4°C in a refrigerator for 48 h, and then filtered with two layers of fine gauze and qualitative filter paper. The obtained solution is the original extract of the above-ground part of Schizonepeta. After sealing it with a sealing film, it was stored in a refrigerator at 4°C for standby. The original extract of the above-ground part of Schizonepeta is called the water extract of the above-ground part of Schizonepeta.

[0082] (2) After drying the collected Schizonepeta inflorescences in an oven, the dried Schizonepeta inflorescences were crushed, passed through a 20-mesh sieve, and distilled water was added for extraction at a mass-to-volume ratio of 8 g / 100 mL of Schizonepeta inflorescences. Static extraction was carried out at 4°C in a refrigerator for 48 h, and then filtered with two layers of fine gauze and qualitative filter paper. The obtained solution is the original extract of Schizonepeta inflorescences. After sealing it with a sealing film, it was stored in a refrigerator at 4°C for standby. Among them, the original extract of Schizonepeta inflorescences is called the water extract of Schizonepeta inflorescences.

[0083] (3)After drying the collected Schizonepeta tenuifolia stems in an oven, the dried Schizonepeta tenuifolia stems are then pulverized, passed through a 20-mesh sieve, and distilled water is added for extraction according to a mass-volume ratio of 8 g / 100 mL of Schizonepeta tenuifolia stems to distilled water. Static extraction is carried out at 4 °C in a refrigerator for 48 h, and then filtered with two layers of fine gauze and qualitative filter paper. The resulting solution is the original extract of Schizonepeta tenuifolia stems. After sealing it with a sealing film, it is stored in a refrigerator at 4 °C for standby. Among them, the original extract of Schizonepeta tenuifolia stems is called the water extract of Schizonepeta tenuifolia stems.

[0084] 3. Preparation of water extract and ethanol extract

[0085] (1)Using the LabTech automatic high-efficiency rapid solvent extractor, weigh 10 g of the dried Schizonepeta tenuifolia stems and put them into the extraction tank. Use distilled water as the first extraction solvent and perform the first extraction according to the set program: extraction temperature 100 °C, extraction pressure 10.34 Mpa, extraction time 4 min, rinsing volume 60% of the sample volume, number of cycles 2 times, and nitrogen purging time 30 sec. After the extraction is completed, collect the extract to obtain the water extract of Schizonepeta tenuifolia stems. Among them, the material ratio of Schizonepeta tenuifolia stems to the first extraction solvent is 10 g:6 mL.

[0086] (2)Using the LabTech automatic high-efficiency rapid solvent extractor, weigh 5 g of the dried Schizonepeta tenuifolia inflorescences and put them into the extraction tank. Use distilled water as the first extraction solvent and perform the first extraction according to the set program: extraction temperature 100 °C, extraction pressure 10.34 Mpa, extraction time 4 min, rinsing volume 60% of the sample volume, number of cycles 2 times, and nitrogen purging time 30 sec. After the extraction is completed, collect the extract to obtain the water extract of Schizonepeta tenuifolia inflorescences. Among them, the material ratio of Schizonepeta tenuifolia inflorescences to the first extraction solvent is 5 g:3 mL.

[0087] (3)Weigh 10 g of the dried Schizonepeta tenuifolia stems and use 75% ethanol by volume as the second extraction solvent. Perform the second extraction according to the set program: extraction temperature 100 °C, extraction pressure 10.34 Mpa, extraction time 5 min, rinsing volume 60% of the sample volume, number of cycles 2 times, and nitrogen purging time 30 sec. After the extraction is completed, collect the extract to obtain the ethanol extract of Schizonepeta tenuifolia stems. Among them, the material ratio of Schizonepeta tenuifolia stems to the second extraction solvent is 10 g:6 mL.

[0088] (4) Weigh 8 g of the dried Schizonepeta tenuifolia Briq. inflorescence, use ethanol with a volume fraction of 75% as the second extraction solvent, and perform the second extraction according to the set procedure: the extraction temperature is 100 °C, the extraction pressure is 10.34 Mpa, the extraction time is 5 min, the rinsing volume is 60% of the sample volume, the number of cycles is 2, and the nitrogen purging time is 30 sec. After the extraction is completed, collect the extract to obtain the ethanol extract of Schizonepeta tenuifolia Briq. inflorescence. Among them, the material ratio of Schizonepeta tenuifolia Briq. inflorescence to the second extraction solvent is 8 g:4.8 mL.

[0089] Put the above-obtained extracts into a solvent storage bottle, seal it with a sealing film, and store it in the refrigerator for later use.

[0090] Among them, the English abbreviation of Labtech is Lab Tech.

[0091] Automatic high-efficiency and rapid solvent extractor: Flex-HPSE.

[0092] 4. Growth inhibition experiment, EC 50

[0093] The growth inhibition experiment is an experiment to measure the effects of the extracts of Schizonepeta tenuifolia Briq. stems and Schizonepeta tenuifolia Briq. inflorescences on the growth of the young roots and hypocotyls of 3 receptor plants.

[0094] Among them, the full English name of EC 50 is Effective Concentration 50%.

[0095] The 3 receptor plants are lettuce, barley, and green vegetables. Among them, green vegetables are Shanghai green.

[0096] The extracts of Schizonepeta tenuifolia Briq. stems and Schizonepeta tenuifolia Briq. inflorescences in this part of the experiment include the water extract of the above-ground part of Schizonepeta tenuifolia Briq., the water extract of Schizonepeta tenuifolia Briq. stems, the water extract of Schizonepeta tenuifolia Briq. inflorescences, the water extract of Schizonepeta tenuifolia Briq. stems, the water extract of Schizonepeta tenuifolia Briq. inflorescences, the ethanol extract of Schizonepeta tenuifolia Briq. stems, and the ethanol extract of Schizonepeta tenuifolia Briq. inflorescences.

[0097] (1) First, dilute the above-prepared water extracts of the above-ground part of Schizonepeta tenuifolia Briq., water extracts of Schizonepeta tenuifolia Briq. inflorescences, and water extracts of Schizonepeta tenuifolia Briq. stems into the following gradient concentrations: 0.04 g / mL, 0.02 g / mL, 0.01 g / mL, 0.008 g / mL, 0.006 g / mL, 0.004 g / mL, 0.003 g / mL, 0.002 g / mL, 0.001 g / mL, and 0.0005 g / mL, and perform the growth inhibition experiment.

[0098] (2) Take 10 g of Schizonepeta tenuifolia stems for the second time, use 75% ethanol by volume as the second extraction solvent, and perform the second extraction treatment according to the above method. The extract of Schizonepeta tenuifolia stems obtained is subjected to rotary evaporation to obtain an extract. Then, the 74 mg of Schizonepeta tenuifolia stem extract is adjusted to concentrations of 0.002 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0012 g / mL, and 0.001 g / mL in sequence to obtain Schizonepeta tenuifolia stem ethanol extracts with different concentrations, and perform growth inhibition experiments.

[0099] (3) Take 5 g of Schizonepeta tenuifolia inflorescences for the second time, use 75% ethanol by volume as the second extraction solvent, and perform the second extraction treatment according to the above method. The extract of Schizonepeta tenuifolia inflorescences obtained is subjected to rotary evaporation to obtain an extract. Then, the 187 mg of Schizonepeta tenuifolia inflorescence extract is adjusted to concentrations of 0.002 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0012 g / mL, and 0.001 g / mL in sequence to obtain Schizonepeta tenuifolia inflorescence ethanol extracts with different concentrations, and perform growth inhibition experiments.

[0100] (4) Take 10 g of Schizonepeta tenuifolia stems for the third time, use distilled water as the first extraction solvent, and perform the first extraction treatment according to the above method. The obtained extract is placed in an evaporating dish and placed in a constant temperature water bath at 100 °C, and left to stand until an extract is formed. Then, the 542 mg of Schizonepeta tenuifolia stem extract is adjusted to concentrations of 0.002 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0012 g / mL, and 0.001 g / mL in sequence to obtain Schizonepeta tenuifolia stem water extracts with different concentrations, and perform growth inhibition experiments.

[0101] (5) Take 5 g of Schizonepeta tenuifolia inflorescences for the third time respectively, use distilled water as the first extraction solvent, and perform the first extraction treatment according to the above method. The obtained extract is placed in an evaporating dish and placed in a constant temperature water bath at 100 °C, and left to stand until an extract is formed. Then, the 415 mg of Schizonepeta tenuifolia inflorescence extract is adjusted to concentrations of 0.002 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0012 g / mL, and 0.001 g / mL in sequence to obtain Schizonepeta tenuifolia inflorescence water extracts with different concentrations, and perform growth inhibition experiments.

[0102] The specific operation of the growth inhibition experiment is as follows:

[0103] Pre-soak lettuce seeds, barley seeds, and green vegetable seeds separately in 9-cm dishes for 24 h for germination. Place filter paper on 33-mm petri dishes and put 5 germinated lettuce seeds, barley seeds, and green vegetable seeds respectively. Add 1 mL of the water extracts of the aerial parts of Nepeta cataria, the water extracts of Nepeta cataria stems, the water extracts of Nepeta cataria inflorescences, the water extracts of Nepeta cataria stems, the water extracts of Nepeta cataria inflorescences, the ethanol extracts of Nepeta cataria stems, and the ethanol extracts of Nepeta cataria inflorescences at each of the above concentrations into each petri dish and place them in a constant temperature device at 25 °C for 2 days. After two days, measure the root length and hypocotyl length of the lettuce seedlings in each petri dish. Plot the elongation of the radicle length or hypocotyl length of the seedlings against each concentration, and define the concentration that inhibits growth by 50% as EC50.

[0104] Among them, the full name of EC50 is Effective Concentration 50%.

[0105] 5. Data processing

[0106] Statistically organize the measured data using Microsoft Excel and then analyze the data for significance and regularity.

[0107] II. Results

[0108] 1. Results and analysis of the sandwich assay

[0109] The research results are shown in Table 1 and Figure 1 as follows.

[0110] Table 1 Effects of extracts of Nepeta cataria inflorescences and stems on the seed germination of three receptor plants by the sandwich method

[0111]

[0112] Note: "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0113] The results in Table 1 and Figure 1 show that in the sandwich method assay, Nepeta cataria inflorescences showed a 100% inhibitory effect on the radicles and hypocotyls of lettuce seedling at concentrations of 1 mg / mL and 5 mg / mL. When barley seeds were used as receptor plants, Nepeta cataria inflorescences showed a weak inhibitory effect on the hypocotyls and a certain promoting effect on the radicles of seedlings at concentrations of 1 mg / mL and 5 mg / mL. When green vegetable seeds were used as receptor plants, Nepeta cataria inflorescences showed a certain inhibitory effect on the radicles and hypocotyls of seedlings at a concentration of 1 mg / mL and a certain promoting effect on the radicles and hypocotyls of seedlings at a concentration of 5 mg / mL. Among them, the concentrations of Nepeta cataria inflorescences at 1 mg / mL and 5 mg / mL refer to the ratio of the mass of dried Nepeta cataria inflorescences to the volume of nutrient agar.

[0114] Table 1 and Figure 2 The results show that when the concentration of Schizonepeta tenuifolia Briq. stems is 1 mg / mL and 5 mg / mL, it shows a strong inhibitory effect on the radicles and hypocotyls of lettuce seedling embryos. When barley seeds are used as the receptor plants, Schizonepeta tenuifolia Briq. stems show a weak inhibitory effect on the radicles and hypocotyls of seedlings at the concentrations of 1 mg / mL and 5 mg / mL. When Brassica chinensis seeds are used as the receptor plants, Schizonepeta tenuifolia Briq. stems show a weak promoting effect on the radicles and hypocotyls of seedlings at the concentrations of 1 mg / mL and 5 mg / mL. Among them, the concentration of Schizonepeta tenuifolia Briq. stems at 1 mg / mL and 5 mg / mL refers to the ratio of the mass of dried Schizonepeta tenuifolia Briq. stems to the volume of nutrient agar.

[0115] 2. Determination Results and Analysis of Growth Inhibition Experiment

[0116] 2.1. Water Extract

[0117] Table 2 and Figure 3 The results show that in the growth inhibition experiment with lettuce as the receptor plant, the water extract of Schizonepeta tenuifolia Briq. inflorescences shows a strong inhibitory effect on the radicles and hypocotyls of seedlings at the concentrations of 0.08 g / mL, 0.04 g / mL, and 0.02 g / mL. The water extract of Schizonepeta tenuifolia Briq. inflorescences shows a certain inhibitory effect on the radicles of seedlings at the concentrations of 0.01 g / mL, 0.008 g / mL, 0.006 g / mL, 0.004 g / mL, 0.003 g / mL, and 0.002 g / mL, and the inhibitory effect gradually becomes stronger with the increase in concentration. The water extract of Schizonepeta tenuifolia Briq. inflorescences shows a certain promoting effect on the hypocotyls at the concentrations of 0.008 g / mL, 0.006 g / mL, 0.004 g / mL, 0.003 g / mL, and 0.002 g / mL, and the promoting effect gradually becomes stronger with the decrease in concentration.

[0118] Table 2 and Figure 4 The results show that in the growth inhibition experiment with lettuce as the receptor plant, the water extract of Schizonepeta tenuifolia Briq. stems shows a 100% inhibitory effect on the radicles and hypocotyls of seedlings at the concentration of 0.08 g / mL. The water extract of Schizonepeta tenuifolia Briq. stems shows a certain inhibitory effect on the radicles of seedlings at the concentrations of 0.04 g / mL, 0.02 g / mL, 0.01 g / mL, 0.008 g / mL, 0.006 g / mL, 0.004 g / mL, 0.003 g / mL, and 0.002 g / mL, and the inhibitory effect gradually becomes stronger with the increase in concentration. The water extract of Schizonepeta tenuifolia Briq. stems shows a certain promoting effect on the hypocotyls at the concentrations of 0.008 g / mL, 0.006 g / mL, 0.004 g / mL, 0.003 g / mL, and 0.002 g / mL, and the promoting effect gradually becomes stronger with the decrease in concentration.

[0119] Table 2 andFigure 5 The results showed that in the growth inhibition experiment with lettuce as the receptor plant, the aqueous extracts of the above-ground parts of Nepeta cataria showed 100% inhibitory effects on the radicles and hypocotyls of seedlings at concentrations of 0.08 g / mL, 0.04 g / mL, and 0.02 g / mL. The aqueous extracts of the above-ground parts of Nepeta cataria showed certain inhibitory effects on the radicles of seedlings at concentrations of 0.01 g / mL, 0.008 g / mL, 0.006 g / mL, 0.004 g / mL, 0.003 g / mL, and 0.002 g / mL, and the inhibitory effects gradually became stronger with the increase in concentration. The aqueous extracts of the above-ground parts of Nepeta cataria showed certain promoting effects on the hypocotyls at concentrations of 0.008 g / mL, 0.006 g / mL, 0.004 g / mL, 0.003 g / mL, and 0.002 g / mL, and the promoting effects gradually became stronger with the decrease in concentration.

[0120] Figure 6 As shown in the results, in the growth inhibition experiment with lettuce as the receptor plant, when comparing the results of the aqueous extracts of the above-ground parts of Nepeta cataria, the aqueous extracts of the stems of Nepeta cataria, and the aqueous extracts of the inflorescences of Nepeta cataria, the inhibitory effects of the aqueous extracts of the above-ground parts of Nepeta cataria and the aqueous extracts of the inflorescences of Nepeta cataria were stronger than those of the aqueous extracts of the stems of Nepeta cataria, which also indicated that the allelochemicals in the inflorescences of Nepeta cataria were stronger than those in other parts.

[0121] Table 2 Effects of aqueous extracts of different parts of Nepeta cataria on the growth of lettuce seedlings at different concentrations

[0122]

[0123] Note: "R" represents the radicle, and "H" represents the hypocotyl.

[0124] Table 3 and Figure 7 The results showed that in the growth inhibition experiment with barley as the receptor plant, the aqueous extracts of the inflorescences of Nepeta cataria showed certain inhibitory effects on the radicles and hypocotyls of seedlings at concentrations of 0.0030 g / mL, 0.0028 g / mL, 0.0026 g / mL, 0.0024 g / mL, 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, and 0.0014 g / mL, and the inhibitory effects also showed a trend of gradually becoming stronger with the increase in concentration. The aqueous extracts of the inflorescences of Nepeta cataria showed certain promoting effects on the radicles and hypocotyls of seedlings at concentrations of 0.0010 g / mL, 0.0008 g / mL, 0.0006 g / mL, and 0.0004 g / mL, and the promoting effects gradually became stronger with the decrease in concentration.

[0125] Table 3 and Figure 8The results showed that in the growth inhibition experiment with barley as the receptor plant, the aqueous extract of Nepeta cataria L. stems showed a weak inhibitory effect on the radicles and hypocotyls of seedlings at concentrations of 0.0030 g / mL, 0.0028 g / mL, 0.0026 g / mL, 0.0024 g / mL, 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, and 0.0014 g / mL; at concentrations of 0.0010 g / mL, 0.0008 g / mL, 0.0006 g / mL, and 0.0004 g / mL, the aqueous extract of Nepeta cataria L. stems showed a weak inhibitory effect on the hypocotyls and a strong promoting effect on the radicles of seedlings.

[0126] Table 3 Effects of aqueous extracts of Nepeta cataria L. inflorescences and Nepeta cataria L. stems at different concentrations on the growth of barley seedlings

[0127]

[0128] Note: "R" represents the radicle, and "H" represents the hypocotyl.

[0129] Table 4 and Figure 9 The results showed that in the growth inhibition experiment with Brassica chinensis L. as the receptor plant, the aqueous extract of Nepeta cataria L. inflorescences showed a certain inhibitory effect on the radicles of seedlings and a promoting effect on the hypocotyls at concentrations of 0.0030 g / mL and 0.0028 g / mL; at concentrations of 0.0026 g / mL and 0.0024 g / mL, the aqueous extract of Nepeta cataria L. inflorescences showed a certain inhibitory effect on the radicles and hypocotyls of seedlings; at concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0010 g / mL, 0.0008 g / mL, 0.0006 g / mL, and 0.0004 g / mL, the aqueous extract of Nepeta cataria L. inflorescences showed a strong inhibitory effect on the radicles of seedlings and a certain promoting effect on the hypocotyls.

[0130] Table 4 and Figure 10 The results showed that in the growth inhibition experiment with Brassica chinensis L. as the receptor plant, the aqueous extract of Nepeta cataria L. stems showed a certain inhibitory effect on the radicles and hypocotyls of seedlings at concentrations of 0.0030 g / mL, 0.0028 g / mL, 0.0026 g / mL, 0.0024 g / mL, 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, 0.0010 g / mL, 0.0008 g / mL, 0.0006 g / mL, and 0.0004 g / mL, and the effect was very unstable. Except for the concentrations of 0.0026 g / mL and 0.0024 g / mL, the remaining concentrations showed a certain promoting effect on the hypocotyls.

[0131] Table 4 Effects of water extracts of Nepeta cataria L. inflorescence and Nepeta cataria L. stem on the growth of Brassica chinensis L. seedlings at different concentrations

[0132]

[0133] Note: "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0134] 2.2 Ethanol extracts

[0135] Table 5 and Figure 11 The results show that in the growth inhibition experiment with Lactuca sativa L. as the receptor plant, the ethanol extract of Nepeta cataria L. inflorescence showed a weak inhibitory effect on the radicle and hypocotyl of seedlings at the concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, and 0.0012 g / mL.

[0136] Table 5 and Figure 12 The results show that in the growth inhibition experiment with Lactuca sativa L. as the receptor plant, the ethanol extract of Nepeta cataria L. stem showed a strong inhibitory effect on the radicle and hypocotyl of Lactuca sativa L. seedlings at the concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, and 0.0012 g / mL, and the inhibitory effect tended to become stronger with the increase of concentration.

[0137] Table 5,[[]] Figure 13 and Figure 14 The results show that in the growth inhibition experiment with Hordeum vulgare L. as the receptor plant, the ethanol extracts of Nepeta cataria L. inflorescence and Nepeta cataria L. stem showed a certain inhibitory effect on the radicle and hypocotyl of seedlings at the concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, and 0.0012 g / mL. And the inhibitory effect of the ethanol extract of Nepeta cataria L. inflorescence was stronger than that of the ethanol extract of Nepeta cataria L. stem.

[0138] Table 5 and Figure 15 The results show that in the growth inhibition experiment with Brassica chinensis L. as the receptor plant, the ethanol extract of Nepeta cataria L. inflorescence showed a weak inhibitory effect on the radicle and hypocotyl of seedlings at the concentration of 0.0020 g / mL; the ethanol extract of Nepeta cataria L. inflorescence showed a promoting effect on the radicle and hypocotyl of seedlings at the concentrations of 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, and 0.0012 g / mL, and the promoting effect tended to become stronger gradually with the decrease of concentration.

[0139] Table 5 and Figure 16The results showed that in the growth inhibition experiment, when using green vegetables as the receptor plants, the ethanol extract of Nepeta cataria L. stems showed a certain promoting effect on the radicles and hypocotyls of seedlings at concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, and 0.0012 g / mL.

[0140] Table 5 Effects of ethanol extracts of Nepeta cataria L. inflorescences and Nepeta cataria L. stems on the growth of receptor plant seedlings at different concentrations

[0141]

[0142] Note: "R" represents the radicle, and "H" represents the hypocotyl.

[0143] 2.3. Water extract

[0144] Table 6 Figure 17 and Figure 18 The results showed that in the growth inhibition experiment, when using lettuce as the receptor plants, the water extract of Nepeta cataria L. inflorescences showed a strong inhibitory effect on the radicles of lettuce seedlings and a certain inhibitory effect on the hypocotyls of lettuce seedlings at concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, and 0.0012 g / mL. Comparing the water extract of Nepeta cataria L. stems and the water extract of Nepeta cataria L. inflorescences, the inhibitory effect of the water extract of Nepeta cataria L. inflorescences on lettuce was stronger than that of the water extract of Nepeta cataria L. stems.

[0145] Table 6 Figure 19 and Figure 20 The results showed that in the growth inhibition experiment, when using barley as the receptor plants, the water extract of Nepeta cataria L. inflorescences and the water extract of Nepeta cataria L. stems showed a certain promoting effect on the radicles and hypocotyls of barley seedlings at concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, and 0.0012 g / mL. Comparing the water extract of Nepeta cataria L. inflorescences and the water extract of Nepeta cataria L. stems, the promoting effect of the water extract of Nepeta cataria L. stems on barley was stronger than that of the water extract of Nepeta cataria L. inflorescences.

[0146] Table 6 Figure 21 and Figure 22 The results showed that in the growth inhibition experiment, when using green vegetables as the receptor plants, the water extract of Nepeta cataria L. inflorescences and the water extract of Nepeta cataria L. stems showed a strong promoting effect on the radicles and hypocotyls of green vegetable seedlings at concentrations of 0.0020 g / mL, 0.0018 g / mL, 0.0016 g / mL, 0.0014 g / mL, and 0.0012 g / mL.

[0147] Table 6 Effects of water extracts of Nepeta cataria L. inflorescences and Nepeta cataria L. stems on the growth of receptor plant seedlings at different concentrations

[0148]

[0149] Note: "R" represents the radicle, and "H" represents the hypocotyl respectively.

[0150] In the sandwich assay, Nepeta cataria inflorescence and Nepeta cataria stem showed 100% inhibitory effects on the growth of lettuce seedlings at concentrations of 1 mg / mL and 5 mg / mL. The inhibitory effects at different concentrations were measured by the EC50 method. Among them, the water extracts of Nepeta cataria inflorescence and Nepeta cataria stem showed inhibitory effects in the concentration range of 0.002 g / mL to 0.08 g / mL, and the inhibitory effects gradually became stronger with the increase in concentration. The water extracts of Nepeta cataria inflorescence and Nepeta cataria stem showed weak inhibitory effects on lettuce at different concentrations, and the inhibitory effects also showed a trend of becoming stronger with the increase in concentration. Nepeta cataria inflorescence and Nepeta cataria stem showed promoting effects on barley and green vegetables as a whole, and the promoting effects gradually became stronger with the decrease in concentration as measured by the EC50 method. The ethanol extracts of Nepeta cataria inflorescence and Nepeta cataria stem showed weak inhibitory effects on the growth of barley seedlings and promoting effects on the growth of green vegetable seedlings at different concentrations.

[0151] The research results of the present invention show that the above-ground part of Nepeta cataria, the water extracts of Nepeta cataria inflorescence and Nepeta cataria stem, the water extracts of Nepeta cataria inflorescence and Nepeta cataria stem, and the ethanol extracts of Nepeta cataria inflorescence and Nepeta cataria stem have significant inhibitory effects on the germination and seedling growth of lettuce seeds, while having significant promoting effects on the germination and seedling growth of barley and green vegetable seeds. Moreover, the volatile substances in Nepeta cataria stem have a stronger inhibitory effect on lettuce than Nepeta cataria inflorescence, indicating that the content of volatile substances with inhibitory effects is higher in Nepeta cataria stem. On the contrary, the volatile substances in Nepeta cataria inflorescence have a stronger promoting effect on barley and green vegetables than Nepeta cataria stem, indicating that the content of volatile substances with promoting effects is higher in Nepeta cataria inflorescence.

[0152] The inhibitory effect of the water extract of Nepeta cataria inflorescence on lettuce is stronger than that of the water extract of Nepeta cataria stem, indicating that the content of volatile substances with inhibitory effects is higher in Nepeta cataria inflorescence. Similarly, the promoting effect of the water extract of Nepeta cataria inflorescence on barley and green vegetables is stronger than that of the water extract of Nepeta cataria stem, indicating that the content of volatile substances with promoting effects is higher in Nepeta cataria inflorescence.

[0153] The inhibitory effect of the water extract and ethanol extract of Nepeta cataria stem on lettuce is stronger than that of the water extract and ethanol extract of Nepeta cataria inflorescence, and the promoting effect on barley and green vegetables is also stronger than that of the water extract and ethanol extract of Nepeta cataria stem, indicating that the content of volatile substances with inhibitory effects and promoting effects is higher in Nepeta cataria stem.

[0154] Therefore, among vegetables and crops, using an appropriate concentration of Nepeta cataria extract or extract solution to inhibit weeds around the required vegetables and crops will also promote the growth of the plants. It can replace the use of chemical reagents and avoid the adverse effects of chemical reagents on the environment and the quality of fruits and vegetables.

[0155] At the same time, the present invention also found through experiments that Nepeta cataria has strong allelopathic potential for weeds. Once there are a large number of Nepeta cataria plants in an environmental protection area with a large variety of plant species, there is a chance of causing relatively serious harm. For example, the growth of plants in this protection area is affected and they cannot grow healthily or even die directly, resulting in a reduction in the number of plant species and an imbalance in the ecological environment. Therefore, Nepeta cataria should be discovered and cleared in a timely manner during management and maintenance. In addition, when certain weeds need to be removed in agriculture, the Nepeta cataria extract or plant residues can be appropriately utilized, which may have a certain removal effect.

[0156] Therefore, among vegetables and crops, using an appropriate concentration of Nepeta cataria extract or extract solution to inhibit weeds around the required vegetables and crops will also promote the growth of the plants. It can replace the use of chemical reagents and avoid the adverse effects of chemical reagents on the environment and the quality of fruits and vegetables.

[0157] The experimental results show that Nepeta cataria has strong allelopathic potential for weeds. Once there are a large number of Nepeta cataria plants in an environmental protection area with a large variety of plant species, there is a chance of causing relatively serious harm. For example, the growth of plants in this protection area is affected and they cannot grow healthily or even die directly, resulting in a reduction in the number of plant species and an imbalance in the ecological environment. Therefore, Nepeta cataria should be discovered and cleared in a timely manner during management and maintenance. In addition, when certain weeds need to be removed in agriculture, the Nepeta cataria extract or plant residues can be appropriately utilized, which may have a certain removal effect.

[0158] It should be noted that when the present invention involves a numerical range, it should be understood that any value between the two endpoints of each numerical range and the two endpoints themselves can be selected. To avoid repetition, the present invention describes preferred embodiments.

[0159] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications to these embodiments once they know the basic creative concept, and these changes and modifications all fall within the scope of all changes and modifications of the present invention.

Claims

1. Use of schizonepeta extract in regulating the growth of crops, characterized in that, The Nepeta extract is used for regulating the seed germination and seedling growth of crops; the regulation includes promotion and / or inhibition; the Nepeta extract includes Nepeta flower inflorescence extract and Nepeta stem extract; The crops are selected from any one or more of lettuce, barley, and Shanghaiqing; The Nepeta flower inflorescence extract includes at least one of Nepeta flower inflorescence water extract, Nepeta flower inflorescence water extract, and Nepeta flower inflorescence ethanol extract; The Nepeta stem extract includes at least one of Nepeta stem water extract, Nepeta stem water extract, and Nepeta stem ethanol extract.

2. Use of schizonepeta extract in regulating the growth of crops, characterized in that, The Nepeta extract is used for regulating the seed germination and seedling growth of crops; the regulation includes promotion and / or inhibition; the Nepeta extract includes Nepeta stem extract; The crops are selected from any one or more of lettuce, barley, and Shanghaiqing; The Nepeta stem extract includes at least one of Nepeta stem water extract, Nepeta stem water extract, and Nepeta stem ethanol extract.

3. Use of schizonepeta extract in regulating the growth of crops, characterized in that, The Nepeta extract is used for regulating the seed germination and seedling growth of crops; the regulation includes promotion and / or inhibition; the Nepeta extract includes Nepeta flower inflorescence extract; The crops are selected from any one or more of barley and Shanghaiqing; The Nepeta stem extract includes at least one of Nepeta stem water extract, Nepeta stem water extract, and Nepeta stem ethanol extract.

4. Use of the schizonepeta extract according to any one of claims 1 to 3 in regulating the growth of crops, characterized in that, The Nepeta flower inflorescence water extract is the filtrate collected after adding water to the Nepeta flower inflorescence for extraction at 4°C, followed by filtration; The Nepeta stem water extract is the filtrate collected after adding water to the Nepeta stem for extraction at 4°C, followed by filtration; The material ratio of the Nepeta flower inflorescence to water and the material ratio of the Nepeta stem to water are both 8g:100mL.

5. Use of the schizonepeta extract according to any one of claims 1 to 3 in regulating the growth of crops, characterized in that, The Nepeta flower inflorescence water extract is the extraction liquid collected by using the Nepeta flower inflorescence as the raw material and water as the first extraction solvent for the first extraction; The Nepeta stem water extract is the extraction liquid collected by using the Nepeta stem as the raw material and water as the first extraction solvent for the first extraction; The material ratio of the Nepeta flower inflorescence to the first extraction solvent is 5g:3mL; the material ratio of the Nepeta stem to the first extraction solvent is 10g:6mL.

6. Use of the schizonepeta extract according to claim 5 in regulating the growth of crops, characterized in that, The conditions for the first extraction are: extraction temperature 100°C, extraction pressure 10.34 Mpa, and extraction time 4 min.

7. Use of the schizonepeta extract according to any one of claims 1 to 3 in regulating the growth of crops, characterized in that, The Nepeta flower inflorescence ethanol extract is the extraction liquid collected by using the Nepeta flower inflorescence as the raw material and 75% ethanol by volume as the second extraction solvent for the second extraction; The Nepeta stem ethanol extract is the extraction liquid collected by using the Nepeta stem as the raw material and 75% ethanol by volume as the second extraction solvent for the second extraction; The material ratio of the Nepeta flower inflorescence to the second extraction solvent is 8g:4.8mL; the material ratio of the Nepeta stem to the second extraction solvent is 10g:6mL.

8. Use of the schizonepeta extract according to claim 7 in regulating the growth of crops, characterized in that, The conditions for the second extraction are: extraction temperature 100°C, extraction pressure 10.34 Mpa, and extraction time 5 min.

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