Application of jujube bud tea extract in preparation of product for improving alcoholic liver injury

Abstract

The invention belongs to the technical field of jujube bud reutilization, and provides application of a jujube bud tea extract in preparation of a product for improving alcoholic liver injury. The alcoholic liver injury is acute alcoholism liver injury. The jujube bud tea extract is a jujube bud tea water extract. The preparation method of the jujube bud tea water extract comprises the following steps: adding 10 times by weight of water into jujube bud tea, carrying out reflux extraction at 100 DEG C for 1 hour, filtering, adding 8 times by weight of water into filter residues, carrying out reflux extraction at 100 DEG C for 45 minutes, filtering, combining two filtrates, and concentrating into 0.3 g.mL <-1 > water decoction according to the crude drug amount. The prepared jujube bud tea water extract has a certain protection effect on the liver of a drunken rat, and can reduce the injury degree of the liver; the jujube bud tea extract has a certain improvement effect on liver injury of rats suffering from acute alcoholism.

Description

Technical Field

[0001] The invention belongs to the technical field of jujube bud recycling, and specifically relates to the application of a jujube bud tea extract in the preparation of a product for improving alcoholic liver damage. Background Art

[0002] my country is a major tea-producing country in the world, with a long history of tea production, a wide distribution of tea-producing areas, huge tea production and a large number of tea companies. China is not only the birthplace of tea, but also the origin of black tea. In addition, my country's red date production accounts for more than 98% of the world's total production, and has an important industrial position.

[0003] The jujube bud tea involved in the present invention is produced in Lin County, Shanxi Province. Lin County is located at 37° north latitude. The area is mostly wild and pollution-free loess hilly gully area, which provides a unique growth environment for Lin County red dates, making the jujubes sweet and sour and rich in nutrition. Jujube bud black tea uses the tender buds and leaves of jujube trees as raw materials, and is refined through nine traditional processes such as picking, withering, rolling, fermentation, sun drying, microwave killing, drying, aroma, grade screening and packaging combined with modern technology.

[0004] The red date planting area in the four counties along the Yellow River in Luliang has reached 1.6 million mu, of which Lin County has a planting area of ​​820,000 mu. According to statistics from the past three years, the theoretical yield of red date buds and leaves per mu of red date trees is about 1,000 kilograms. However, due to the high crown of the red date trees and the gullies in the growing area, the actual effective picking rate is about one-fourth, that is, an average of 250 kilograms can be picked per mu, and the total amount that can be picked in the county is about 100 million kilograms per year. The development of red date bud black tea not only realizes the comprehensive utilization of the red date forest economy and turns waste into treasure, but also provides stable green economic benefits for red date farmers.

[0005] Jujube bud black tea belongs to the category of economic forest medicinal tea, with warm nature and sweet taste. At present, there are still few studies on the active ingredients and nutritional value of jujube bud black tea. It is urgent to further explore the potential effects of jujube bud tea and clarify its medicinal and health value. It is urgent to provide important scientific basis and reference value for the development and application of jujube bud black tea in multiple fields. Summary of the invention

[0006] The invention provides an application of a jujube bud tea extract in preparing a product for improving alcoholic liver damage.

[0007] The present invention is achieved by the following technical scheme: application of a jujube bud tea extract in the preparation of a product for improving alcoholic liver damage.

[0008] Furthermore, the alcoholic liver injury is acute alcohol poisoning liver injury.

[0009] Furthermore, the jujube bud tea extract is a jujube bud tea water extract.

[0010] The preparation method of the jujube bud tea water extract is as follows: add 10 times the weight of water to the jujube bud tea, reflux extract at 100°C for 1 hour, filter, add 8 times the weight of water to the filter residue, reflux extract at 100°C for 45 minutes, filter, combine the two filtrates, and concentrate to 0.3 g·mL according to the crude drug amount. -1 Decoction of.

[0011] The jujube bud tea water extract prepared by the invention has a certain protective effect on the liver of drunk rats and can reduce the degree of liver damage; the jujube bud tea extract has a certain improvement effect on the liver damage of rats with acute alcohol poisoning. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 Middle: a: Effect of jujube leaf extract on ALT, a serum index of drunken rats; b: Effect of jujube leaf extract on AST, a serum index of drunken rats; c: Effect of jujube leaf extract on TG, a serum index of drunken rats; d: Effect of jujube leaf extract on TC, a serum index of drunken rats; e: Effect of jujube leaf extract on HDL-C, a serum index of drunken rats; f: Effect of jujube leaf extract on LDL-C, a serum index of drunken rats; g: Effect of jujube leaf extract on T-bil-V, a serum index of drunken rats; h: Effect of jujube leaf extract on D-bil-V, a serum index of drunken rats; i; Effect of jujube leaf extract on GLU, a serum index of drunken rats; j: Effect of jujube leaf extract on the time of drunkenness in the serum of drunken rats; k: Effect of jujube leaf extract on the sobering time of drunken rats; 1: Effect of jujube leaf extract on ADH, a serum index of drunken rats; m: Effect of jujube leaf extract on ALDH, a serum index of drunken rats; #p indicates a significant comparison with the normal group; *P indicates a significant comparison with the model group; Figure 2 Middle: a: HE staining results of the liver of the drunk rat Nor. group; b: HE staining results of the liver of the drunk rat Con. group. c: HE staining results of the liver of the drunk rat L-JBT group; d: HE staining results of the liver of the drunk rat M-JBT group. e: HE staining results of the liver of the drunk rat H-JBT group; f: HE staining results of the liver of the drunk rat NKD group; Figure 3Middle: a: Effect of jujube leaf extract on serum index ALT in rats with acute alcohol poisoning; b: Effect of jujube leaf extract on serum index AST in rats with acute alcohol poisoning; c: Effect of jujube leaf extract on serum index TG in rats with acute alcohol poisoning; d: Effect of jujube leaf extract on serum index TC in rats with acute alcohol poisoning; e: Effect of jujube leaf extract on serum index HDL-C in rats with acute alcohol poisoning; f: Effect of jujube leaf extract on serum index LDL-C in rats with acute alcohol poisoning; g: Effect of jujube leaf extract on serum index T-bil-V in rats with acute alcohol poisoning; h: Effect of jujube leaf extract on serum index D-bil-V in rats with acute alcohol poisoning; 1: Effect of jujube leaf extract on serum index GLU in rats with acute alcohol poisoning; j: Effect of jujube leaf extract on serum drunkenness time in rats with acute alcohol poisoning; k: Effect of jujube leaf extract on sobering time in rats with acute alcohol poisoning; 1: Effect of jujube leaf extract on serum index ADH in rats with acute alcohol poisoning; m: Effect of jujube leaf extract on serum index ALDH in rats with acute alcohol poisoning. #p indicates significant comparison with the normal group; *P indicates significant comparison with the model group; Figure 4 Middle: a: HE staining results of liver in Nor. group of rats with acute alcohol intoxication; b: HE staining results of liver in Con. group of rats with acute alcohol intoxication; c: HE staining results of liver in L-JBT group of rats with acute alcohol intoxication; d: HE staining results of liver in M-JBT group of rats with acute alcohol intoxication; e: HE staining results of liver in H-JBT group of rats with acute alcohol intoxication; t: HE staining results of liver in NKD group of rats with acute alcohol intoxication. DETAILED DESCRIPTION

[0013] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0014] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the disclosure and materials cited therein are hereby incorporated by reference.

[0015] Technical equivalents to the specific embodiments described that are apparent to those skilled in the art using no more than routine experimentation are intended to be encompassed by this application.

[0016] The experimental methods in the following examples are all conventional methods unless otherwise specified. The instruments and equipment used in the following examples are all conventional laboratory instruments and equipment unless otherwise specified; the experimental materials used in the following examples are all purchased from conventional biochemical reagent stores unless otherwise specified.

[0017] 1. Reagents and instruments: Sinocare blood glucose meter and test strips (Batch No.: 2J01E210813, Sinocare Biosensor Co., Ltd., Changsha, China); alkaline phosphatase (ALP) assay kit (AMP buffer method) (Batch No.: 140324005, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); γ-glutamyltransferase (γ-GT) assay kit (IFCC method) (Batch No.: 140424002, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); total bilirubin (T-Bil) assay kit (vanadate Oxidation method) (Batch number: 140624010, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); Direct bilirubin (D-Bil) assay kit (vanadate oxidation method) (Batch number: 140724011, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); Alanine aminotransferase (ALT) assay kit (IFCC method) (Batch number: 140124019, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); Aspartate aminotransferase (AST) assay kit (IFCC method) (Batch number: 140224007, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); High-density lipoprotein cholesterol (HDL-C) assay kit (direct method) (Lot No.: 142124014, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); Low-density lipoprotein cholesterol (LDL-C) assay kit (direct method) (Lot No.: 142024009, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); Glucose (GLU) assay kit (glucose oxidase method) (Lot No.: 141524012, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); total cholesterol (TC) assay kit (oxidase method) (Batch No.: 141624005, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); triglyceride (TG) assay kit (oxidase method) (Batch No.: 141724016, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China); uric acid (UA) assay kit (uricase-peroxidase method) (Batch No.: 141224011, Shenzhen Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China).

[0018] Haiwang Jinzun Tablets (Batch No. SC12722020028862, No. 1999, Haiwang Road, Zhongxin Food Zone, Chaluhe Town, Yongji County, Jilin City, Jilin Province, China); 56-degree Red Star Erguotou (Batch No. SC11511160310087, Liuquxiang Branch of Beijing Red Star Co., Ltd., Beijing, China); Fenjiu (Batch No. SC11514118200634, Xianghua Village, Fenyang City, Shanxi Province, China); Sodium carboxymethyl cellulose (CMC) (Batch No. SC20131011600816, Shanghai Changguang Enterprise Development Co., Ltd., Shanghai, China); Alcohol dehydrogenase (ADH) test kit (Batch No. 20240920, Nanjing Jiancheng Bioengineering Institute, Nanjing, China); Aldehyde dehydrogenase (ALDH) detection kit (Batch No. 20240920, Nanjing Jiancheng Bioengineering Institute, Nanjing, China).

[0019] GeneGnome system (GeneGnome XRQ, Gene, USA); Imag5eJ (ImageJ2, NIH, USA); GraphPad Prism (version 5.0, GraphPad Software, La Jolla, CA, USA).

[0020] 2. Experimental animals: Male SD rats (Beijing Biotechnology Co., Ltd., Beijing, China; Animal License No.: SCXK (Beijing) 2019-0010), SPF grade, 5-7 weeks old, body weight 180-220 g. Housing conditions: temperature (23±2)℃, relative humidity (50±10)%, lighting conditions 12 h light / 12 h dark cycle, free access to food and water, all mice used were adaptively fed for 1 week before the experiment.

[0021] 3. Experimental Methods 1. Preparation of jujube bud tea extract: Take an appropriate amount of jujube bud tea, add 10 times the amount of water, reflux extraction for 1 h, filter, add 8 times the amount of water to the residue, reflux extraction for 45 min, filter, combine the two filtrates, and concentrate to 0.3 g mL -1 (calculated based on the amount of raw herbs) as a decoction.

[0022] 2. Preparation and administration of drunk rat and acute alcohol intoxication rat models: (1) Animal grouping and group treatment: After one week of adaptive feeding, 48 rats were randomly selected and divided into a blank control group, a model control group, a positive control Neptune Jinzun tablets group, and a low-, medium-, and high-dose group of jujube bud tea extract, with 8 rats in each group.

[0023] The disappearance of the mouse "righting reflex" was used as the indicator of intoxication. The number of intoxicated and dead mice was recorded and the intoxication rate and mortality rate were calculated. The dose with the highest intoxication rate and the lowest mortality rate was used as the optimal oral gavage dose for the acute intoxication model of mice. Each experimental group was gavaged with the corresponding test substance for 2 weeks.

[0024] (2) Establishment of rat acute alcohol intoxication model: The blank control group mice were gavaged with an equal volume of distilled water, once a day for 14 consecutive days; the model control group mice were gavaged with 56° Red Star Erguotou at 20 mL / kg body weight once to establish the rat drunkenness model; the positive control group was given 3 g / kg of Haiwang Jinzun tablets, and the low, medium and high gavage doses of jujube bud tea extract were 1.1 g / kg, 2.2 g / kg and 4.4 g / kg, respectively, once a day for 14 consecutive days. The mice were fasted for 12 hours before the last administration. One hour after the last administration, each group was gavaged with 56° Red Star Erguotou at 20 mL / kg body weight once. Compared with the normal control group, the blood ethanol and acetaldehyde volume fractions of rats in the model control group were increased, with statistically significant differences (P < 0.01), confirming the success of the drunkenness model.

[0025] (3) Experiment on acute intoxication model of mice: 35 rats were randomly divided into 5 groups, namely normal control group, model control group, positive control group, low-dose group and high-dose group, with 7 rats in each group. The rats were given oral administration. The dose of positive control group was 3 g / kg, and the low, medium and high oral doses of jujube bud tea extract were 1.1 g / kg, 2.2 g / kg and 4.4 g / kg, respectively. 0.5 h after oral administration, except for the normal group, the rats in the model group and the drug group were given oral administration of 52% liquor at a dose of 1.5 mL / 100 g (rat body weight) once to establish the rat acute alcohol intoxication model.

[0026] 4. Results and Discussion 1. Effects of jujube bud tea extract on various indicators of drunken rats A. Effects of jujube bud tea extract on serum parameters of drunken rats: Results are as follows Figure 1 As shown in Figure 2, compared with the normal group, the alanine aminotransferase ALT in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, M-JBT ( P <0.001)、H-JBT( P <0.01) and NKD( P <0.001) group ALT was significantly decreased ( Figure 1 a).

[0027] Compared with the normal group, the level of aspartate aminotransferase AST in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P<0.001)、M-JBT( P <0.001) and NKD( P <0.001) group AST was significantly decreased ( Figure 1 b).

[0028] Compared with the normal group, the triglyceride TG in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group TG was significantly decreased ( Figure 1 c).

[0029] Compared with the normal group, the total cholesterol TC in the Con. group was significantly increased ( P <0.01). Compared with the Con. group, L-JBT ( P <0.01) and NKD( P <0.01) group TC was significantly reduced ( Figure 1 d).

[0030] Compared with the normal group, there was no significant difference in high-density lipoprotein cholesterol HDL-C in the Con. group. Compared with the Con. group, H-JBT ( P <0.001) group HDL-C significantly decreased ( Figure 1 e).

[0031] Compared with the normal group, the low-density lipoprotein cholesterol (LDL-C) in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group LDL-C significantly decreased ( Figure 1 f).

[0032] Compared with the normal group, the total bilirubin T-Bil-V in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P <0.05) group D-Bil-V was significantly decreased ( Figure 1 g).

[0033] Compared with the normal group, the direct bilirubin D-Bil-V in the Con. group was significantly increased ( P<0.001). Compared with the Con. group, H-JBT ( P <0.01) group D-Bil-V was significantly decreased ( Figure 1 h).

[0034] Compared with the normal group, the glucose GLU in the Con. group increased significantly ( P <0.001). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.01) and NKD( P <0.001) group GLU significantly decreased ( Figure 1 i).

[0035] Compared with the Con. group, L-JBT( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group had significantly longer drunkenness time ( Figure 1 g).

[0036] Compared with the Con. group, L-JBT( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group had significantly shorter sobering time ( Figure 1 k).

[0037] Compared with the normal group, the alcohol dehydrogenase ADH in the Con. group was significantly decreased ( P <0.001). Compared with the Con. group, 0.5hL-JBT ( P <0.001) and H-JBT( P <0.01) group ADH increased significantly; 2h L-JBT ( P <0.001) group had a significant increase in ADH ( Figure 1 L).

[0038] Compared with the normal group, the 0.5h Con. group; acetaldehyde dehydrogenase ALDH was significantly decreased ( P <0.001). Compared with the Con. group, 0.5h L-JBT ( P <0.01) group ALDH was significantly increased ( Figure 1 m).

[0039] B. Analysis of HE staining results of drunken rats: Liver Nor._ HE-200x: Microscopically, the hepatocytes were loosely arranged and normal in shape, with no obvious edema or inflammation, and damage = 0 ( Figure 2 a). Liver Con._ HE-200x: Severe edema can be seen in the liver cells under the microscope. The cells are swollen and the cytoplasm is loose and lightly stained (black arrows). Severe ballooning is often seen (green arrows). Injury = 2 ( Figure 2 b). Liver L-JBT_ HE-200x: Microscopically, mild edema of hepatocytes is widely observed, with swollen cells and loose and lightly stained cytoplasm (black arrows), and occasional focal necrosis of hepatocytes in the lobules (green arrows). Damage = 1 ( Figure 2 c). Liver M-JBT_ HE-200x: Severe edema, cell swelling, loose and lightly stained cytoplasm (black arrows) can be seen in the liver cells under the microscope, and severe ballooning (green arrows) are often seen. Damage = 2 ( Figure 2 d). Liver H-JBT_ HE-200x: Microscopically, moderate edema of hepatocytes, cell swelling, loose and lightly stained cytoplasm (black arrows), damage = 2 ( Figure 2 e). Liver NKD_ HE-200x: Severe edema, cell swelling, loose and lightly stained cytoplasm (black arrow), rarely severe to balloon-like degeneration (green arrow), injury = 2 ( Figure 2 f).

[0040] 2. Effects of jujube bud tea extract on various parameters of rats with acute alcohol poisoning A. Effects of jujube bud tea extract on serum indexes of drunken rats: Compared with the normal group, ALT in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001) group ALT was significantly decreased ( Figure 3 a). Compared with the normal group, AST in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group AST was significantly decreased ( Figure 3 b). Compared with the normal group, TG in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT(P <0.001) and NKD( P <0.001) group TG was significantly decreased ( Figure 3 c). Compared with the normal group, TC in the Con. group was significantly increased ( P <0.01). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group TC was significantly reduced ( Figure 3 d). Compared with the normal group, there was no significant difference in HDL-C in the Con. group. Compared with the Con. group, M-JBT ( P <0.001) and NKD( P <0.01) group HDL-C significantly decreased ( Figure 3 e). Compared with the normal group, LDL-C in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, M-JBT ( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group LDL-C significantly decreased ( Figure 3 f). Compared with the normal group, D-Bil-V in the Con. group was significantly increased ( P <0.01). Compared with the Con. group, NKD ( P <0.05) group D-Bil-V was significantly decreased ( Figure 3 g). Compared with the normal group, T-Bil-V in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group T-Bil-V was significantly decreased ( Figure 3 h). Compared with the normal group, GLU in the Con. group was significantly increased ( P <0.001). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group GLU significantly decreased ( Figure 3 i). Compared with the Con. group, L-JBT (P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group had significantly longer drunkenness time ( Figure 3 j). Compared with the Con. group, L-JBT ( P <0.001)、M-JBT( P <0.001)、H-JBT( P <0.001) and NKD( P <0.001) group had significantly shorter sobering time ( Figure 3 k). Compared with the normal group, the ADH in the 0.5h Con. group was significantly decreased ( P <0.001). Compared with the Con. group, 0.5h L-JBT ( P <0.05) and H-JBT( P <0.05) group ADH increased significantly; 2h L-JBT ( P <0.05) group had a significant increase in ADH ( Figure 3 l). Compared with the normal group, ALDH in the 0.5hCon. group was significantly decreased ( P <0.05) Figure 3 m).

[0041] B. Analysis of HE staining results of drunken rats: Liver Nor._ HE-200x: Microscopically, the hepatocytes were loosely arranged and normal in shape, with no obvious edema or inflammation, and damage = 0 ( Figure 4 a). Liver Con._ HE-200x: Severe edema can be seen in the liver cells under the microscope, the cells are swollen, and the cytoplasm is loose and lightly stained (black arrows). Damage = 2 ( Figure 4 b). Liver L-JBT_ HE-200x: Microscopically, the hepatocytes around the central vein are moderately edematous, the cells are swollen, and the cytoplasm is loose and lightly stained (black arrows). Damage = 1 ( Figure 4 c). Liver M-JBT_ HE-200x: Severe edema, cell swelling, loose and lightly stained cytoplasm (black arrows) can be seen in the liver cells under the microscope. Damage = 2 ( Figure 4 d). Liver H-JBT_ HE-200x: Severe edema, cell swelling, loose and lightly stained cytoplasm (black arrows) can be seen in the liver cells under the microscope. Damage = 2 ( Figure 4 e). Liver NKD_ HE-200x: Microvesicular fatty degeneration of hepatocytes is rare under the microscope, and tiny lipid droplets can be seen in the hepatocytes (black arrows), damage = 1 ( Figure 4 f).

[0042] In summary, the experimental results show that the results of the hangover experiment showed that the jujube bud tea water extract can significantly shorten the recovery time of the drunken state of rats, showing a good hangover effect. Effect of jujube bud tea water extract on serum metabolism of rats with alcoholic liver injury Metabolomics analysis showed that jujube bud tea water extract can regulate the metabolic pathways of rats with alcoholic liver injury and improve their metabolic state. Analysis of the physiological and pathological effects of jujube bud tea water extract on rats with alcoholic liver injury Serum biochemical indicators and liver tissue pathological examination showed that jujube bud tea water extract can alleviate pathological changes such as hepatocyte necrosis and fatty degeneration in rats with alcoholic liver injury. Effect of jujube bud tea water extract on the expression of antioxidant response pathways and inflammatory pathways in liver tissue of rats with alcoholic liver injury Western blot results showed that jujube bud tea water extract can upregulate the expression level of proteins related to the antioxidant response pathway, and downregulate the expression level of proteins related to the inflammatory pathway, thereby exerting antioxidant and anti-inflammatory effects. Jujube bud tea extract has a certain protective effect on the liver of drunken rats and can reduce the degree of liver damage; jujube bud tea extract has a certain improvement effect on liver damage in rats with acute alcohol poisoning.

[0043] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. Application of a jujube bud tea extract in the preparation of a product for improving alcoholic liver damage.

2. The use according to claim 1, characterized in that: The alcoholic liver injury is acute alcohol poisoning liver injury.

3. The use according to claim 1, characterized in that: The product for improving alcoholic liver injury is a medicine or food for improving alcoholic liver injury.

4. The use according to claim 1 or 2, characterized in that: The jujube bud tea extract is a jujube bud tea water extract.

5. The use according to claim 4, characterized in that: The preparation method of the jujube bud tea water extract is as follows: add 10 times the weight of water to the jujube bud tea, reflux extract at 100°C for 1 hour, filter, add 8 times the weight of water to the residue, reflux extract at 100°C for 45 min, filter, combine the two filtrates, and concentrate them into a water decoction of 0.3 g·mL-1 according to the amount of crude drug.

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