An antioxidant and anti-aging composition, its preparation method and application

Abstract

The application provides an antioxidant and anti-aging composition and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine.The composition comprises the following components in parts by weight: 25-40 parts of radix astragali, 5-10 parts of angelica sinensis, 18-25 parts of mulberry, 8-15 parts of hawthorn, 8-15 parts of malt and 2-5 parts of pericarpium citri reticulatae; wherein the weight ratio of radix astragali to angelica sinensis is 5:1, and the weight ratio of mulberry, hawthorn and malt is 18-25:8-15:8-15. The composition has the effects of antioxidation and anti-aging.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of traditional Chinese medicine, and particularly relates to an antioxidant and anti-aging composition, a preparation method and application thereof. BACKGROUND

[0002] Aging refers to the gradual decline of the functions of various organs of the human body. The aging of the body is mainly manifested in the skin, cardiovascular system, nervous system, digestive system, etc. Among them, the aging of the nervous system will lead to slow reaction, such as decline in learning, memory, judgment, and motor coordination, and a series of changes in the nervous system, and the sensitivity of body temperature regulation will also decrease, and some elderly people are also prone to Alzheimer's disease. The human body contains a variety of endogenous antioxidant enzymes and low molecular weight antioxidants, which can capture free radicals that harm the human body, so that the production and elimination of free radicals in the body are in a dynamic balance, so that the body is not harmed. With age, the activity of antioxidant enzymes such as superoxide dismutase (SOD) in the human body decreases, and the ability to scavenge oxygen free radicals in the body decreases, and the high concentration of oxygen free radicals acts on nucleic acids, proteins, cell membranes, enzymes, etc. to cause irreversible damage, thereby leading to physiological disorders and even pathological changes in the body.

[0003] Chinese patent application CN112273649A discloses an antioxidant and anti-aging composition, a preparation method and application thereof, the composition comprising the following components: American ginseng, brown algae peptide, black fruit of Ribes nigrum L., beta-nicotinamide mononucleotide, Lycium barbarum polysaccharide, Hippophae rhamnoides polysaccharide, Morus nigra polysaccharide and Malus sieversii polysaccharide. However, it contains brown algae peptide, beta-nicotinamide mononucleotide and other high-priced raw materials, and contains Lycium barbarum polysaccharide, Hippophae rhamnoides polysaccharide, Morus nigra polysaccharide and Malus sieversii polysaccharide, which limits the application of the composition to the population with sugar intolerance.

[0004] Chinese patent application CN117918515A discloses an anti-fatigue nutritional preparation comprising the following components: Radix Astragali 25-35 parts by weight, Radix Codonopsis 10-20 parts by weight, Angelica sinensis 5-10 parts by weight, Pericarpium Citri Reticulatae 5-15 parts by weight, Fructus Jujubae 8-15 parts by weight, Fructus Crataegi 8-15 parts by weight, Flos Chrysanthemi 8-15 parts by weight, Rhizoma Dioscoreae 10-20 parts by weight, Endod 2-8 parts by weight, and Malt 2-8 parts by weight. The above-mentioned application has many components, and does not disclose the effect of anti-aging and antioxidant.

[0005] Although there are many compositions with antioxidant and anti-aging effects at present, there is a lack of a pure traditional Chinese medicine preparation composition with antioxidant and anti-aging effects, which has low cost, few components, is suitable for a wide range of people, and has good effect. SUMMARY

[0006] The present application provides an antioxidant and anti-aging composition, a preparation method and application thereof, and has the advantages of good antioxidant and anti-aging effect, low cost, few components, and wide application population.

[0007] To achieve the above object, the technical scheme adopted by the present application is as follows:

[0008] The first object of the present application is to provide an antioxidant and anti-aging composition, and the raw material components are as follows in terms of weight parts: 25-40 parts of Astragalus, 5-10 parts of Angelica, 18-25 parts of mulberry, 8-15 parts of hawthorn, 8-15 parts of malt, and 2-5 parts of dried tangerine or orange peel, wherein the weight ratio of Astragalus to Angelica is 5:1, and the weight ratio of mulberry, hawthorn and malt is 18-25:8-15:8-15.

[0009] Preferably, the raw material components are as follows in terms of weight parts: 28-35 parts of Astragalus, 6-8 parts of Angelica, 18-22 parts of mulberry, 8-12 parts of hawthorn, 8-12 parts of malt, and 2-4 parts of dried tangerine or orange peel.

[0010] Further preferably, the raw material components are as follows in terms of weight parts: 30 parts of Astragalus, 6 parts of Angelica, 20 parts of mulberry, 10 parts of hawthorn, 10 parts of malt, and 2 parts of dried tangerine or orange peel.

[0011] Preferably, the composition further comprises a pharmaceutically acceptable excipient.

[0012] Further preferably, the excipient is selected from any one or more of dextrin, malt dextrin, soluble starch and lactose.

[0013] The second object of the present application relates to a preparation method of the above-mentioned composition, comprising the following steps:

[0014] (1) weighing the raw materials, repeatedly decocting with water, filtering, and combining the filtrates;

[0015] (2) concentrating the combined filtrate into extract, adding an excipient, and drying to obtain the composition.

[0016] Preferably, in step (1), the number of repeated decocting with water is 1-3 times, the amount of water added in each decocting is 6-10 times the amount of raw materials, and the decocting time of each decocting is 1-2 hours.

[0017] Preferably, in step (2), the extract is a traditional Chinese medicine extract containing 2g / mL of crude drug.

[0018] The third object of the present application relates to the use of the above-mentioned composition in the preparation of an antioxidant and anti-aging drug.

[0019] Preferably, the dosage form of the medicine is not limited to pills, capsules, granules, oral liquids, powders, tablets, lozenges, pastes, etc. For different dosage forms, suitable pharmaceutical carriers in the art can be selected.

[0020] Huangqi, derived from Shennong's Herbal Classic, has been praised since ancient times as a good medicine for tonifying qi and raising yang, consolidating the exterior and stopping sweating, promoting water excretion and swelling reduction, generating fluid and nourishing blood, removing stagnation and relieving arthralgia, removing toxins and pus, and promoting wound healing and tissue regeneration. In the treatment of anti-aging in the elderly, Huangqi, with its outstanding qi-tonifying effect, plays the role of monarch drug. It not only significantly enhances the body's immune function and improves the body's resistance to disease, but also promotes the production of qi and blood, improves the body's anti-fatigue ability, and provides stable energy support for the brain, which is conducive to the improvement of learning and memory ability, thereby delaying the aging process of the body.

[0021] Danggui, a holy medicine for tonifying blood and regulating menstruation, is derived from Shennong's Herbal Classic. It is matched with Huangqi in a ratio of one to five, and is derived from Danggui Buxue Decoction created by Li Dongyuan in the Jin and Yuan Dynasties. The two complement each other and jointly produce the effect of tonifying qi, tonifying blood and promoting blood circulation. Danggui, with its functions of tonifying blood, regulating menstruation, relieving pain, moistening the intestines and promoting defecation, is used together with Huangqi, greatly enhancing the tonifying blood effect and effectively improving the pale complexion, dizziness and other deficiency symptoms caused by deficiency of qi and blood in the elderly. At the same time, the blood-activating property of Danggui helps to unblock blood vessels and promote blood circulation, including microcirculation in the brain, providing sufficient oxygen and nutrients for the brain. In traditional Chinese medicine theory, blood deficiency may lead to memory decline. Danggui, through tonifying blood and promoting blood circulation, improves the supply of nutrients to the brain, thereby indirectly promoting the recovery and improvement of brain function, including learning and memory ability.

[0022] Mudan, a good product for nourishing yin and blood, generating fluid and moistening dryness, and nourishing the liver and improving eyesight, plays the role of ministerial drug in this prescription, assisting Huangqi and Danggui to further consolidate the foundation of tonifying blood and nourishing yin. The liver-nourishing and eyesight-improving effect of Mudan is particularly suitable for the treatment of visual impairment, dizziness and tinnitus caused by liver and kidney yin deficiency in the elderly.

[0023] Shanzha, an expert in digesting and resolving food accumulation, promoting blood circulation and reducing fat and softening blood vessels, not only enhances the spleen-strengthening and food-digesting function of this prescription, but also promotes the digestion and absorption of the elderly. Its unique lipid-lowering and vascular-softening effect adds new strength to the prevention of cardiovascular and cerebrovascular diseases and the delay of aging.

[0024] Dama, i.e. malt, is a good medicine for digesting and strengthening the stomach, resolving milk and swelling, and soothing the liver and relieving depression. It complements Shanzha and optimizes the spleen and stomach function of the elderly, promoting digestion and absorption. The liver-soothing and depression-relieving property of malt is particularly good at regulating emotions and relieving physical discomfort caused by emotional fluctuations in the elderly, embodying the wisdom of emotional therapy in traditional Chinese medicine. In traditional Chinese medicine theory, the liver is in charge of dispersion and is closely related to emotional activities. Malt, through soothing the liver and relieving depression, improves the emotional state, helps the brain to maintain clarity and focus, and thereby indirectly improves learning and memory efficiency.

[0025] The dried tangerine or orange peel is a good product for regulating qi, invigorating the spleen, drying dampness, resolving phlegm and promoting qi and relieving pain, and is used as an auxiliary medicine in the prescription, which can help digestion, dry dampness and resolve phlegm, and improve the symptoms of cough, excessive phlegm, dizziness and memory decline caused by internal resistance of phlegm and dampness in middle-aged and old people.

[0026] In summary, the modified prescription is based on the angelica blood tonic soup, and the astragalus and angelica are used as the monarch, which supplements qi and nourishes blood; the mulberry is used as the minister, which enhances blood tonification and yin nourishment; the hawthorn and malt are used as the auxiliary, which invigorates the spleen, removes food retention and soothes the liver and relieves depression; and the dried tangerine or orange peel is used as the messenger, which regulates qi, resolves phlegm, promotes qi and relieves pain. The whole prescription has the effects of supplementing qi and nourishing blood, nourishing yin and moistening dryness, removing food retention and resolving accumulation, promoting qi and removing blood stasis, soothing the liver and relieving depression, and reducing fat and softening the tube, and aims to enhance the physical strength and immunity of middle-aged and old people, and improve memory and learning ability, and is an optimal prescription for anti-aging prevention and treatment and antioxidant of middle-aged and old people.

[0027] Compared with the prior art, the composition provided by the application can improve the learning and memory function of aging mice, reduce the MDA and SOD levels of brain tissue, reduce the oxidative stress level of brain tissue, reduce the generation of beta-glycosidase, and slow down the aging degree of brain tissue, and the effect is remarkable. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 : Partial motion trajectory diagram of the Morris water maze experiment of the mice in each group;

[0029] Figure 2 : Positioning navigation experiment result diagram of the mice in each group;

[0030] Figure 3 : Spatial exploration experiment result of the mice in each group on the last day;

[0031] Figure 4 : Platform crossing frequency result diagram of the mice in each group;

[0032] Figure 5 : Mice brain tissue MDA level detection result diagram;

[0033] Figure 6 : Mice brain tissue SOD level detection result diagram;

[0034] Figure 7 : Mice brain slice ROS fluorescence detection result (Note: The number in the lower right corner of each diagram is 1.25 mm);

[0035] Figure 8 : Mice brain slice beta-glycosidase staining result diagram. DETAILED DESCRIPTION

[0036] The following non-limiting examples can provide a more complete understanding of the application to one of ordinary skill in the art, but are not intended to limit the scope of the application in any way. The following description is merely exemplary of the application and is not intended to limit the scope of the application, as described in the appended claims.

[0037] When a numerical range is given in the examples, it is understood that, unless the application indicates otherwise, each numerical range is selected from two endpoints and any number between the two endpoints. Unless otherwise defined, all technical and scientific terms used in the application have the same meaning as commonly understood by one of ordinary skill in the art to which the application belongs.

[0038] The application is further described below in the manner of specific examples. The various chemical reagents and raw materials used in the examples of the application are obtained through conventional commercial channels unless otherwise specified.

[0039] Composition 1: An antioxidant and anti-aging composition, the raw material components are as follows in terms of weight parts: Radix Astragali 30 parts, Angelica 6 parts, mulberry 20 parts, haw 10 parts, malt 10 parts, and dried tangerine or orange peel 2 parts. The preparation method is as follows: 8 times the weight of the raw materials of pure water is boiled at 600W high fire, then changed to 200W low fire for 2 hours, filtered to collect the medicinal liquid, and the residue is boiled again with 8 times the volume of pure water for 1 hour, filtered to collect the medicinal liquid, and the medicinal liquids are combined and concentrated to a traditional Chinese medicine extract containing 2g / mL of raw medicinal materials by a rotary evaporator.

[0040] Experimental Example 1: In vitro antioxidant and anti-aging experiment

[0041] 1. Animal modeling and drug administration:

[0042] 1) Select 8-week-old male C57BL6 / J mice (20-25g), adaptively feed for one week, and then randomly divide into normal group (Control group), model group (D-gal group), positive drug group (D-gal+DNP), composition 1 low-dose group (D-gal+DL), and composition 1 high-dose group (D-gal+DH group). Except for the normal group, which is injected with the same volume of water for injection every day, the other groups are injected with D-galactose (D-gal) intraperitoneally for 10 weeks. Starting from the third week, the D-gal+DNP, D-gal+DL, and D-gal+DH groups are given corresponding doses of drugs by gavage every day for 8 weeks. Behavioral tests are conducted at the 11th week, and the experimental animals are sacrificed after the test.

[0043] 2) Dose: D-galactose: 120mg / kg; Composition 1 low dose: 6mg / kg / day; Composition 1 high dose: 12mg / kg / day; Positive drug: donepezil (DNP), dose: 3mg / kg / day.

[0044] 2. Morris water maze experiment:

[0045] The Morris water maze experiment includes three parts: platform visible period adaptation training, platform invisible period positioning navigation, and spatial exploration. The water temperature is controlled at 22 ± 2 ℃ during the experiment. The specific experimental steps are as follows:

[0046] 1) Build the water maze facility. On the first day of the experiment, the platform visible period experiment is conducted, and the mice are trained to find the safe platform. The mice are raised in the behavior laboratory 10 hours in advance for environmental adaptation. The swimming pool is divided into four quadrants, and the platform is located in one of the quadrants, with a height of 1 cm above the water surface. The pool water is kept transparent, and a black marker is placed on the platform to ensure that the mouse can see the platform in the water. First, place the mouse on the safe platform for 20 seconds, then gently support the mouse with your hand, and place the mouse in the water in the four quadrant positions for four experiments respectively. When placing the mouse, let the mouse's head face the pool wall, and enter the water gently and slowly, and let the animal's head enter the water first. Let the mouse freely explore the safe platform in the water for 1 minute, and if it reaches the platform and stops for 10 seconds within 1 minute, it is considered to have found the platform successfully. If it does not find the platform within 1 minute, manually guide it to the safe platform, and let the mouse stay on the platform for 15 seconds or more. Each mouse is subjected to 4 experiments, and after the experiment is completed, the mouse is immediately taken out of the pool and dried with a towel, and placed in a mouse cage next to the heating. After screening out mice with slow swimming speed due to injury to the motor organ, long time in water, and fast swimming speed, subsequent experiments are conducted.

[0047] 2) Start the positioning navigation experiment on the second day of the experiment. The safe platform is still located in the same quadrant as the first day. In this stage, non-toxic dye is poured into the water and mixed to make the pool completely turbid, and the platform is located 0.5 cm below the water surface to ensure that the mouse cannot see the safe platform in the water. Place the mouse facing the pool wall and gently into the water, let the mouse determine the position by the markings on the pool wall, and freely explore the safe platform for 1 minute. If it reaches the safe platform and stays for 10 seconds within 1 minute, it is considered to have successfully climbed the platform. If it does not find the platform within 1 minute, manually guide it to the safe platform, and let the mouse stay on the platform for 15 seconds before proceeding to the next experiment. The positioning navigation experiment is conducted continuously for 4 days, and the mouse enters the water in four different quadrants each day. The time taken by the mouse to find and climb the platform is called the latency period. If the mouse does not climb the safe platform within 1 minute, the latency period is recorded as 60 seconds. The latency period of each mouse is recorded and the entire process of image data is retained.

[0048] 3) The spatial exploration experiment was performed from the 7th day of the experiment. The safe platform in the water maze was removed, and a non-toxic dye was used to turbid the pool. The mice were placed in the water at the site farthest from the original safe platform position, facing the pool wall. The mice were gently placed in the water, and they determined their position by the markings on the pool wall. They were allowed to explore freely for 1 minute. The time spent by the mice in the original platform quadrant (target quadrant) and the time spent crossing the platform were recorded within 60 seconds.

[0049] The partial motion trajectories of the mice in each group during the Morris water maze experiment are shown in Figure 1 The motion trajectories and speeds of the mice in the water maze were recorded. If a mouse exhibited a state of floating in the water without swimming in 3 or more experiments out of 4 experiments, it was directly excluded and did not participate in subsequent water maze experiments.

[0050] Figure 2 The experimental results show that in the positioning navigation experiment, the latency of all mice gradually decreased with the increase of the experimental days. However, the latency of the D-gal group was still significantly longer than that of the other experimental groups. The latency of the D-gal+DH group was significantly reduced compared to the D-gal group (P<0.01).

[0051] As shown in Figure 3 In the spatial exploration experiment on the last day, the time spent by the mice in the platform quadrant of the D-gal group was significantly reduced. The D-gal+DH group showed a significant increase in the time spent in the platform quadrant compared to the D-gal group (P<0.05).

[0052] As shown in Figure 4 The number of times the mice crossed the platform in the D-gal group was significantly reduced. Except for the D-gal+DL group, all other groups showed a significant increase in the number of times they crossed the platform compared to the D-gal group.

[0053] 3. Detection of MDA levels in mouse brain tissue:

[0054] The steps for measuring the MDA level in brain tissue are as follows: ① Tissue collection: After the mice are anesthetized and sacrificed, the brain tissue is quickly removed. ② Homogenization and extraction: The brain tissue samples are homogenized with MDA extraction solution, centrifuged at 4°C, and the supernatant is used as the analysis sample. ③ MDA determination: A certain amount of supernatant is taken, and an equal volume of thiobarbituric acid (TBA) solution is added. The mixture is heated in a boiling water bath for 60 minutes to allow the MDA to react with TBA to form a red-brown 3,5,5-trimethyl oxazole-2,4-dione. ④ Absorbance measurement: After cooling the reaction solution to room temperature, the absorbance is measured at a wavelength of 532 nm using a spectrophotometer. ⑤ Statistical analysis: The concentration and content of MDA in the sample are calculated, and the results are plotted using GraphPad Prism.

[0055] As shown in Figure 5As shown, the MDA level in the D-gal group mice was elevated, and all other groups except the D-gal+DL group showed a significant decrease in MDA level compared to the D-gal group.

[0056] 4. Detection of SOD levels in mouse brain tissue:

[0057] Steps for determining SOD levels in brain tissue: ① Sample collection: After anesthetizing and euthanizing mice, brain tissue was quickly collected. ② Homogenization and extraction: The obtained brain tissue sample was homogenized with SOD extraction solution, centrifuged at 12000×g at 4℃, and the supernatant was used as the analytical sample. An appropriate amount of brain tissue sample was weighed and placed in a pre-cooled mortar. ③ SOD activity determination: The sample was divided into a test group and a control group. An appropriate amount of pure water and SOD working solution were added to each group. An appropriate amount of homogenate was added to the test group, and an equal amount of SOD extraction solution was added to the control group as a blank control. ④ Absorbance measurement: After sample addition, the sample was incubated at 37℃ for 30 min, and the absorbance value was measured at a wavelength of 450nm. ⑤ Statistical analysis: SOD activity was calculated according to the formula: Enzyme activity = [(ODC-ODS) / (ODC)×V / Vs] / W, where V is the total volume of the sample extract (mL), Vs is the volume of the sample extract taken during the measurement (mL), and W is the sample weight (g). The calculated SOD activity values ​​were statistically analyzed.

[0058] like Figure 6 As shown, the SOD activity of mice in the D-gal group was decreased, while the SOD activity of all other groups was significantly increased compared to the D-gal group.

[0059] 5. ROS fluorescence in mouse brain slices:

[0060] Specific steps for ROS fluorescence analysis of brain tissue: ① Tissue collection: Anesthetized mice were perfused with pre-cooled PBS, and the whole brain was immediately removed. ② Fixation: The whole brain of the mice was pre-fixed in paraformaldehyde. ③ Sectioning: Brain slices were prepared to a thickness of 20 μm using an OTC implanter and a cryostat. ④ Fluorescent probe: An appropriate volume of ROS staining solution was added to ensure complete coverage of the tissue, and the tissue was incubated in a humidified chamber at 37°C for 30 min. ⑤ Observation and analysis: The stained brain tissue sections were observed under a fluorescence microscope.

[0061] like Figure 7 As shown, the ROS level of mice in the D-gal group was increased, and the ROS levels of all other groups except the D-gal+DL group were significantly lower than those in the D-gal group.

[0062] 6. β-Glucosidase staining of mouse brain sections:

[0063] The specific steps of brain tissue β-galactosidase staining are as follows: ① sample collection: the heart of the anesthetized mouse is perfused with pre-cooled PBS, and the whole brain of the mouse is immediately taken out. ② fixation: the whole brain of the mouse is pre-fixed in paraformaldehyde. ③ sectioning: embedding is performed using OTC, and a brain slice with a thickness of 20 μm is prepared by a freezing microtome. ④ staining: a proper volume of β-galactosidase staining fixative is added to ensure sufficient coverage of the tissue, and the room temperature fixation is performed for at least 15 minutes. The tissue is washed by immersion in PBS for 3 times, each for no less than 5 minutes. The PBS is absorbed, and a proper amount of β-galactosidase staining working solution (X-gal) is added. Incubation is performed at 37°C overnight, and the evaporation is prevented by using a preservative film to seal.

[0064] As shown in Figure 8 the D-gal group, and the β-galactosidase staining degree of each of the other groups is shallower than that of the D-gal group.

[0065] Example 1

[0066] An antioxidant and anti-aging composition, the raw material components according to weight parts are as follows: 25 parts of Astragalus, 5 parts of Angelica, 18 parts of mulberry, 8 parts of hawthorn, 8 parts of malt, and 2 parts of dried tangerine or orange peel.

[0067] Example 2

[0068] An antioxidant and anti-aging composition, the raw material components according to weight parts are as follows: 40 parts of Astragalus, 8 parts of Angelica, 25 parts of mulberry, 15 parts of hawthorn, 15 parts of malt, and 5 parts of dried tangerine or orange peel.

[0069] Example 1 and Example 2 are obtained by the following preparation method:

[0070] Boil 8 times the weight of pure water of the raw material at 600W high fire, change to 200W low fire after boiling and cook for 2 hours, filter to collect the medicinal liquid, and decoct the residue with 8 times the volume of pure water for 1 hour, filter to collect the medicinal liquid, and combine the medicinal liquids and concentrate by a rotary evaporator to a traditional Chinese medicine extract containing 2g / mL of crude drug.

[0071] Finally, it should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Simple modifications or equivalent replacements of the technical solutions of the present application made by ordinary skilled in the art do not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. An antioxidant, anti-aging composition, characterized in that, The raw material components are, by weight parts: 25-40 parts of Huangqi, 5-10 parts of Danggui, 18-25 parts of Sangsheng, 8-15 parts of Shanzha, 8-15 parts of Maiya, and 2-5 parts of Chenpi, The weight ratio of Huangqi to Danggui is 5:1, and the weight ratio of Sangsheng, Shanzha and Maiya is 18-25:8-15:8-15.

2. The composition of claim 1, wherein, The raw material components are, by weight parts: 28-35 parts of Huangqi, 6-8 parts of Danggui, 18-22 parts of Sangsheng, 8-12 parts of Shanzha, and 8-12 parts of Maiya, and 2-4 parts of Chenpi.

3. The composition of claim 1, wherein, The raw material components are, by weight parts: 30 parts of Huangqi, 6 parts of Danggui, 20 parts of Sangsheng, 10 parts of Shanzha, 10 parts of Maiya, and 2 parts of Chenpi.

4. The composition according to any one of claims 1 to 3, wherein The composition further comprises a pharmaceutically acceptable excipient.

5. The composition of claim 4, wherein The excipient is selected from any one or more of dextrin, soluble starch and lactose.

6. A process for the preparation of a composition according to any one of claims 1 to 5, characterised in that, The method comprises the following steps: (1) weighing the raw materials, repeatedly decocting with water, filtering, and combining the filtrates; (2) concentrating the combined filtrate to a extract, adding an excipient, and drying to obtain the product.

7. The production method according to claim 6, wherein In step (1), the number of times of repeated decocting with water is 1-3, the amount of water added for each decocting is 6-10 times the amount of raw materials, and the decocting time for each decocting is 1-2 hours.

8. The production method according to claim 6, wherein In step (2), the extract is a traditional Chinese medicine extract containing 2 g / mL of crude drug.

9. Use of the composition of any one of claims 1-5 or prepared by the method of any one of claims 6-8 in the preparation of an anti-oxidation, anti-aging drug.

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