Protein solution purification device and purification method

Abstract

The invention discloses a protein solution purification device and method, the protein solution purification device comprises a chromatography device and a dialysis container connected through a four-way pipe, the chromatography device comprises a chromatography tube half tube B, a chromatography tube half tube A and a plurality of blocking nets, the chromatography tube half tube B and the chromatography tube half tube A are detachably connected, and the blocking nets are lapped between the chromatography tube half tube B and the chromatography tube half tube A, and thin-layer matrixes are arranged on the blocking nets; a dialysis device is arranged in the dialysis container, and a buffer solution inlet pipe opening and a dialysis liquid outlet pipe are arranged on one side of the dialysis container. Through cooperative use of the chromatography half-tube A and the chromatography half-tube B, the chromatography half-tube B and the chromatography half-tube A are detachably connected, and the blocking net and the thin-layer matrix are overlapped in the chromatography half-tube A and the chromatography half-tube B, the problem that a stationary phase is not easy to replace in a chromatography experiment is solved, a user can flexibly adjust the number of the blocking net and the thin-layer matrix according to experiment requirements, and the experiment efficiency is improved. The separation effect is improved, a large amount of protein solution can be treated at a time, the blocking net, the thin-layer matrix and the dialysis device are allowed to be replaced according to needs, and the applicability is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of protein solution purification, and in particular to a protein solution purification device and a purification method. Background Art

[0002] Protein expression and purification plays a vital role in biological science research and biopharmaceuticals. With the growing demand for high-purity proteins, researchers are paying more and more attention to effective purification technologies. In the prior art, dialysis bags are a commonly used protein purification tool. The principle is based on the characteristics of semipermeable membranes, which allow small molecules to pass through and block large molecular proteins. However, dialysis bags have many limitations in practical applications, especially when dealing with large volumes of protein solutions. Their operation is complicated and inefficient, which has become an important challenge faced by researchers. Specifically, the volume of dialysis bags is limited, and when dealing with a large amount of protein, multiple bags are often needed at the same time, which not only increases the complexity of operation, but also may cause solution leakage and cross-contamination. In addition, the process of replacing the dialysate is cumbersome and time-consuming. Researchers need to remove the dialysis bag from the device, dump the old liquid and reinstall it, which significantly affects the purification efficiency. In addition, the existing dialysis equipment lacks automated control and cannot accurately control key parameters such as dialysis time, liquid flow rate and temperature, thereby limiting the purification effect and quality.

[0003] In addition to dialysis bags, chromatography technology is also an important protein purification method. However, chromatography technology also faces a series of problems. In chromatography experiments, the replacement of the stationary phase is often complicated and time-consuming. Traditional devices fail to consider the convenience of replacement, resulting in experimenters spending a lot of time and energy when replacement is needed. At the same time, the amount of stationary phase used is difficult to accurately control, which affects the separation effect of the protein, and the irregularity of the chromatography path further reduces the separation efficiency. In addition, the column pressure problem cannot be ignored. The increase in pressure causes the stationary phase to accumulate, further reducing the chromatography efficiency.

[0004] In view of the shortcomings of the existing technology, a new type of automated and efficient protein purification device is urgently needed. Summary of the invention

[0005] The technical problem to be solved by the present invention is: how to improve the efficiency of protein solution chromatography and dialysis, and further realize automation.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] A protein solution purification device comprises a chromatography device and a dialysis container connected by a four-way pipe, wherein the chromatography device comprises a detachably connected chromatography tube half-tube B and a chromatography tube half-tube A, and a plurality of screens overlapped between the two, wherein the screens are provided with a thin layer matrix;

[0008] A dialysis device is arranged inside the dialysis container, a buffer inlet pipe port and a dialysis liquid outlet pipe are arranged on one side of the dialysis container, and a buffer release pipe port is arranged at the bottom of the dialysis device.

[0009] The present invention uses a chromatography half-tube A and a chromatography half-tube B in combination. The chromatography half-tube B is detachably connected to the chromatography half-tube A, and a screen and a thin-layer matrix are overlapped inside the two, thereby solving the problem that the stationary phase in the chromatography experiment is difficult to replace. The user can flexibly adjust the number of screens and thin-layer matrices according to experimental requirements, thereby improving the separation effect. The device can process a large amount of protein solution at a time, and allows the screen, thin-layer matrix, and dialysis device to be replaced as needed, thereby enhancing applicability. The overall design fully considers the convenience of operation, and simplifies the operation steps from the introduction of samples to the collection after purification, and then to the cleaning and maintenance of each component, thereby greatly reducing the workload and operation difficulty of the experimenter.

[0010] As a further solution of the present invention: the chromatography tube half tube B and the chromatography tube half tube A are both arc-shaped plate structures, wherein the interiors of the chromatography tube half tube B and the chromatography tube half tube A are provided with a plurality of groups of blocks for overlapping the blocking nets along the vertical direction.

[0011] As a further solution of the present invention: U-shaped slide grooves are arranged on both sides of the chromatography tube half tube B, and slide rails corresponding to the U-shaped slide grooves are arranged on both sides of the chromatography tube half tube A, and the U-shaped slide grooves of the chromatography tube half tube B can be slidably connected to the outer side of the slide rails.

[0012] As a further solution of the present invention: the chromatography device also includes a liquid inlet tube cover, a liquid storage tube and a liquid outlet tube cover, the liquid inlet tube cover is installed on the top of the liquid storage tube, the chromatography tube half tube B and the chromatography tube half tube A are installed at the bottom of the liquid storage tube, and the liquid outlet tube cover is installed at the bottom of the chromatography tube half tube B and the chromatography tube half tube A, wherein the liquid outlet tube cover is connected to the dialysis container through a four-way tube.

[0013] As a further solution of the present invention: the chromatography device includes a dialysis inlet tube connected to the four-way pipe, the dialysis inlet tube is connected to a dialysis bag through a rubber ring, a dialysis bag support net is arranged on the outside of the dialysis bag, the dialysis bag is arranged in a circuitous shape, and the other end of the dialysis bag is connected to the dialysis outlet tube.

[0014] As a further solution of the present invention: the dialysis inlet tube and the dialysis outlet tube have the same structure, and the sides of the dialysis inlet tube and the dialysis outlet tube connected to the dialysis bag are both provided with triangular flat interfaces, and the other sides of the dialysis inlet tube and the dialysis outlet tube are both cylindrical interfaces.

[0015] As a further solution of the present invention: the four-way pipe includes a first pipe opening of the four-way pipe, a second pipe opening of the four-way pipe, a third pipe opening of the four-way pipe and a fourth pipe opening of the four-way pipe, the first pipe opening of the four-way pipe and the third pipe opening of the four-way pipe form a connected horizontal pipe, the second pipe opening of the four-way pipe and the fourth pipe opening of the four-way pipe form a connected vertical pipe, wherein the second pipe opening of the four-way pipe is connected to the bottom of the chromatography device, the first pipe opening of the four-way pipe is connected to the dialysis container, and a control valve is provided in each pipe opening.

[0016] As a further solution of the present invention: the control valves in the third pipe port of the four-way pipe and the fourth pipe port of the four-way pipe are closed, and the protein solution enters the chromatography device and the dialysis container for chromatography and dialysis treatment.

[0017] As a further solution of the present invention: close the control valves in the second and fourth ports of the four-way pipe, and connect a circulation pump between the buffer release port and the third port of the four-way pipe to perform circulating dialysis of the protein solution.

[0018] The present invention also discloses a purification method of a protein solution purification device, comprising the following steps:

[0019] Close the buffer release port, and pass the buffer (or dialysate) into the dialysis container from the buffer inlet port;

[0020] Close the four-way tube connecting the chromatography device and the dialysis device, open it, and pass the purified protein solution sample into the chromatography device. After passing through the thin layer matrix and dialysis bag for chromatography and dialysis in sequence, the dialysis effluent is collected from the dialysis effluent outlet.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. The present invention solves the problem of difficult replacement of stationary phase in chromatography experiments by using the liquid storage tube, chromatography half tube A and chromatography half tube B in coordination, pushing and pulling the external slide rails and U-shaped slide grooves of chromatography half tube B and chromatography half tube A to open and close, and overlapping the blocking net and thin layer matrix inside the two.

[0023] 2. The present invention uses the combination of the slot, the screen and the thin layer matrix inside the chromatography half tube B, so that the user can change the number of screens and thin layer matrices installed according to experimental requirements, thereby solving the problem of difficult quantification of the use of the stationary phase in the traditional chromatography process and the problem of irregular chromatography path. Compared with the traditional chromatography, the present device has a more obvious separation effect on proteins in the sample;

[0024] 3. The present invention creatively uses a screen to fix a thin layer of matrix (or stationary phase), solving the problem of increased column pressure and low chromatography efficiency due to accumulation of stationary phase caused by pressure during chromatography;

[0025] 4. The present invention cooperates with the dialysis liquid inlet tube, dialysis bag, and dialysis bag support net to make the liquid cross section of the protein solution flat during dialysis, thereby increasing the surface area / volume ratio of the protein solution during dialysis and improving the dialysis efficiency;

[0026] 5. The present invention increases the path for the dialysate to flow through by the coordination of the support platform, the groove, and the dialysis bag support net in the dialysis container, thereby improving the dialysis effect and solving the problem of the time-consuming dialysis of traditional protein solutions;

[0027] 6. The present invention can process a large amount of protein solution at a time, wherein the blocking net, thin layer matrix and dialysis bag can be replaced according to the sample to be processed, thereby improving the applicability of the device;

[0028] In summary, the protein solution purification device of the present invention has important innovative significance and application value in the field of protein expression and purification, and can provide more efficient and convenient technical support for research and production in related fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 This is a schematic diagram of the structure of a protein solution purification device according to an embodiment of the present invention;

[0030] Figure 2 It is a partial cross-sectional view of a protein solution purification device according to an embodiment of the present invention;

[0031] Figure 3 It is a schematic diagram of the structure of the chromatography device according to an embodiment of the present invention;

[0032] Figure 4 It is a partial exploded view of a chromatography device according to an embodiment of the present invention;

[0033] Figure 5 It is a top view of the chromatography tube half-tube B and the chromatography tube half-tube A after being connected in the embodiment of the present invention;

[0034] Figure 6 A partial cross-sectional view of a liquid inlet pipe cover according to an embodiment of the present invention;

[0035] Figure 7 This is a schematic diagram of the structure of the barrier net and the thin layer matrix according to an embodiment of the present invention;

[0036] Figure 8 An exploded view of a chromatography device according to an embodiment of the present invention;

[0037] Fig. 9 It is a schematic diagram of the structure of a chromatography device and a dialysis device according to an embodiment of the present invention;

[0038] Fig.10 Schematic diagram of a support platform in a dialysis container according to an embodiment of the present invention;

[0039] Fig.11This is a schematic diagram of the structure of a dialysis bag support network according to an embodiment of the present invention;

[0040] Fig.12 This is a schematic diagram of the buffer solution (dialysis fluid) circulation of an embodiment of the present invention;

[0041] Fig.13 This is a schematic diagram of the dialysis cycle of a sample solution according to an embodiment of the present invention;

[0042] Description of reference numerals:

[0043] 1. Liquid inlet pipe cover; 2. Liquid storage pipe; 3. Chromatography tube half tube B; 4. Chromatography tube half tube A; 6. Liquid outlet pipe cover; 7. Bracket; 8. Four-way pipe; 81. First pipe opening of four-way pipe; 82. Second pipe opening of four-way pipe; 83. Third pipe opening of four-way pipe; 84. Fourth pipe opening of four-way pipe; 9. Dialysis liquid inlet pipe; 10. Buffer inlet pipe opening; 11. Dialysis bag; 12. Support platform; 13. Chromatography frame; 14. Dialysis liquid outlet pipe; 15. Chromatography device; 16. Buffer release pipe opening; 17. Rubber ring 1; 18. Rubber ring 2; 19. Stopper; 20. Blocking net; 21. Slide rail; 22. Dialysis container; 23. Groove; 24. Dialysis bag support net; 25. Thin layer matrix; 26. U-shaped slide; 27. Rubber ring bundle. DETAILED DESCRIPTION

[0044] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0045] Reference Figure 1 and Figure 2 A protein solution purification device includes a chromatography frame 13 and a dialysis container 22 connected to the chromatography frame 13, the dialysis container 22 is installed on one side of the chromatography frame 13, a chromatography device 15 is installed inside the chromatography frame 13, the bottom of the chromatography device 15 is supported by a bracket 7, the bracket 7 is installed on the chromatography frame 13, and the chromatography device 15 is higher than the dialysis container 22, the chromatography device 15 is connected to the dialysis container 22 through a four-way pipe 8, and the protein solution can enter the chromatography device 15 and the dialysis container 22 in turn for chromatography treatment and dialysis treatment.

[0046] Reference Figure 3 and Figure 4The chromatography device 15 includes a liquid inlet pipe cover 1, a liquid storage tube 2, a chromatography tube half tube B 3, a chromatography tube half tube A 4 and a liquid outlet pipe cover 6, wherein the liquid inlet pipe cover 1 is installed on the top of the liquid storage tube 2, the chromatography tube half tube B 3 and the chromatography tube half tube A 4 are sleeved on the outer side of the bottom of the liquid storage tube 2, a rubber ring 18 is provided at the connection position of the chromatography tube half tube B 3 and the chromatography tube half tube A 4 and the liquid storage tube 2, the bottom of the chromatography tube half tube B 3 and the chromatography tube half tube A 4 are installed in the liquid outlet pipe cover 6, and a rubber ring is also provided at the connection position of the chromatography tube half tube B 3 and the chromatography tube half tube A 4 and the liquid outlet pipe cover 6.

[0047] Further, see Figure 6 and Figure 8 A groove body connected to the liquid storage tube 2 is opened inside the liquid inlet pipe cover 1, and a rubber ring 17 is arranged in the groove body. A liquid inlet pipe is opened at the middle position of the top of the liquid inlet pipe cover 1, wherein the diameter of the liquid inlet pipe is smaller than the inner diameter of the liquid inlet pipe cover 1; the inner diameter of the liquid inlet pipe cover 1 is larger than the outer diameter of the liquid storage tube 2, so the liquid storage tube 2 can be inserted into the internal groove body of the liquid inlet pipe cover 1 and sealed by the rubber ring 17.

[0048] Furthermore, the liquid storage tube 2 is an overall cylindrical structure, wherein the top of the liquid storage tube 2 is inserted into the liquid inlet tube cover 1, and the bottom of the liquid storage tube 2 is provided with a concave cylinder, the outer diameter of the concave cylinder is smaller than the outer diameter of the liquid storage tube 2, the chromatography tube half tube B 3 and the chromatography tube half tube A 4 are installed from both sides of the concave cylinder at the bottom of the liquid storage tube 2, and a rubber ring 18 is sleeved at the connection position between the top of the chromatography tube half tube B 3 and the chromatography tube half tube A 4 and the concave cylinder.

[0049] Further, the chromatography tube half tube B 3 and the chromatography tube half tube A 4 are both arc-shaped plate-like structures, and the chromatography tube half tube B 3 and the chromatography tube half tube A 4 can be spliced ​​into a complete cylindrical structure; outwardly convex U-shaped slide grooves 26 are arranged on both sides of the chromatography tube half tube B 3, and a section of the bottom of the chromatography tube half tube B 3 without the U-shaped slide groove 26 is reserved for inserting into the liquid outlet pipe cover 6 to realize the installation of the liquid outlet pipe cover 6 and the chromatography tube half tube B 3; a plurality of groups of blocks 19 are equidistantly arranged in the vertical direction on the inner side of the chromatography tube half tube B 3, and a blocking net 20 is installed on the block 19, and a blocking net 20 is installed on the blocking net A thin layer matrix 25 is installed on 20. It should be noted that the specific number of groups of blocks 19 is determined according to the height of the chromatography tube half tube B 3 and actual needs, and the present application does not make any limitation. Therefore, the blocking net 20 and the thin layer matrix 25 can be adjusted in vertical position and quantity as needed. The user can flexibly adjust the number of the blocking net 20 and the thin layer matrix 25 according to experimental requirements, which solves the problem of difficult quantification of the use of the stationary phase in the traditional chromatography process and the problem of irregular chromatography path. Compared with traditional chromatography, the present device has a more obvious separation effect on proteins in the sample.

[0050] Reference Figure 5, slide rails 21 corresponding to the U-shaped slide groove 26 are arranged on both sides of the chromatography tube half tube A 4. When the chromatography tube half tube A 4 is installed, the slide rails 21 can be aligned with the U-shaped slide groove 26 from the bottom of the chromatography tube half tube B 3, that is, the slide rails 21 are inserted into the U-shaped slide groove 26 (such as Figure 5 ), move the chromatography tube half A 4 upward until it is connected to the chromatography tube half B 3;

[0051] A section without the slide rail 21 is reserved at the bottom of the chromatography tube half A 4 for inserting into the liquid outlet pipe cover 6 (such as Figure 8 ), realize the installation of the liquid outlet pipe cover 6 and the chromatography tube half tube A 4;

[0052] Several groups of blocks are also equidistantly arranged in the vertical direction on the inner side of the chromatography tube half tube A 4, and the blocks correspond to the positions of the blocks in the chromatography tube half tube B 3, and are used to overlap the blocking net 20 and the blocks 19 together;

[0053] Furthermore, the bottoms of the chromatography tube half tube B 3 and the chromatography tube half tube A 4 can be inserted into the liquid outlet tube cover 6 and connected to the liquid outlet tube cover 6, and rubber rings are sleeved at the positions where the chromatography tube half tube B 3 and the chromatography tube half tube A 4 are connected to the liquid outlet tube cover 6 to ensure stability and sealing during connection.

[0054] Reference Figure 4 and Figure 5 A groove body connected to the chromatography tube half-tube B 3 and the chromatography tube half-tube A 4 is provided inside the liquid outlet pipe cover 6, and a liquid outlet pipe is provided at the middle position of the bottom of the liquid outlet pipe cover 6, wherein the diameter of the liquid outlet pipe is smaller than the inner diameter of the liquid outlet pipe cover 6; the inner diameter of the liquid outlet pipe cover 6 is larger than the outer diameter of the cylinder body formed by the chromatography tube half-tube B 3 and the chromatography tube half-tube A 4, so the chromatography tube half-tube B 3 and the chromatography tube half-tube A 4 can be inserted into the internal groove body of the liquid outlet pipe cover 6 and sealed by a rubber ring.

[0055] Reference Fig.12 and Fig.13 The four-way pipe 8 includes a first four-way pipe opening 81, a second four-way pipe opening 82, a third four-way pipe opening 83 and a fourth four-way pipe opening 84. The first four-way pipe opening 81 and the third four-way pipe opening 83 form a connected horizontal pipe, and the second four-way pipe opening 82 and the fourth four-way pipe opening 84 form a connected vertical pipe, wherein the second four-way pipe opening 82 is connected to the liquid outlet pipe at the bottom of the liquid outlet pipe cover 6, and the first four-way pipe opening 81 is connected to the dialysis container 22, and a control valve is provided in each pipe opening.

[0056] Reference Figure 2 and Fig. 9A dialysis inlet pipe 9, a dialysis bag 11, a rubber ring bundle 27 and a dialysis bag support net 24 are arranged inside the dialysis container 22, a buffer inlet pipe port 10 and a dialysis outlet pipe 14 are arranged on one side of the dialysis container 22, and a buffer release pipe port 16 is arranged at the bottom of the dialysis device.

[0057] Furthermore, one end of the dialysis inlet tube 9 is connected to the first tube port 81 of the four-way tube 8, and the dialysis inlet tube 9 is connected to the dialysis bag 11 through the rubber ring bundle 27. A dialysis bag support net 24 is arranged on the outside of the dialysis bag 11. The dialysis bag support net 24 is a mesh plate. The dialysis bag support net 24 is supported by the support platform 12 on the inner wall of the dialysis container 22. A groove 23 is provided on the support platform 12 for supporting the dialysis bag 11. The dialysis bag 11 is arranged in a circuitous shape, and the other end thereof is connected to the dialysis outlet tube 14. The design of the circuitous dialysis bag 11 increases the path for the dialysate to flow, improves the dialysis effect, and solves the problem of long time consumption of traditional protein solution dialysis.

[0058] Furthermore, the dialysis inlet tube 9 and the dialysis outlet tube 14 have the same structure, and a triangular flat interface is provided on one side of the dialysis inlet tube 9 and the dialysis outlet tube 14 connected to the dialysis bag 11. The design here makes the liquid cross-section of the protein solution flat during dialysis, increases the surface area / volume ratio of the protein solution during dialysis, and improves the dialysis efficiency; the other side of the dialysis inlet tube 9 and the dialysis outlet tube 14 are both cylindrical interfaces.

[0059] The specific operating principles of this application are as follows:

[0060] Step 1: Select the corresponding number of matrix thin layers 25 and blocking nets 20 according to the sample solution to be purified, put the matrix thin layer 25 into the blocking net 20, put the blocking net 20 into the block 19 inside the chromatography half-tube B 3, connect the chromatography half-tube B 3 to the chromatography half-tube A 4 along the external U-shaped slide 26, connect the lower part to the liquid outlet pipe cover 6, connect the upper part to the liquid storage pipe 2, and connect the upper part of the liquid storage pipe 2 to the liquid inlet pipe cover 1; after the connection is completed, place the whole on the bracket 7 and connect the second pipe port 82 of the four-way pipe 8, and the first pipe port 81 of the four-way pipe 8 is connected to the circular pipe port of the dialysis inlet pipe 9;

[0061] At this time, close the control valves in the third pipe opening 83 of the cross-way pipe and the fourth pipe opening 84 of the cross-way pipe;

[0062] Step 2: Select the corresponding dialysis bag 11 according to the required purified sample, insert one end of the dialysis bag 11 into the flat interface of the dialysis inlet tube 9, and then seal the connection with a rubber ring 27, lay the dialysis bag 11 along the dialysis bag support net 24, and insert the end into the flat interface of the dialysis outlet tube 14, and seal the connection with a rubber ring 27;

[0063] Step 3: Close the buffer release nozzle 16, and pass the buffer (or dialysate) from the buffer inlet nozzle 10 into the dialysis container 22 to immerse the dialysis bag 11. Then, according to the experimental requirements, an external circulation pump system can be connected to circulate the buffer to achieve a better purification effect (such as Fig.12 shown);

[0064] Step 4: The purified sample is introduced into the chromatography device 15 through the liquid inlet cap 1, and then passes through the thin layer matrix 25 and the dialysis bag 11 in sequence, undergoes chromatography and dialysis, and then flows out from the dialysis outlet 14 for collection;

[0065] Then, according to the experimental requirements, an external circulation pump system can be connected to perform dialysis circulation of the sample solution to achieve a better purification effect. Note: During this dialysis circulation step, the control valves of the second pipe port 82 and the fourth pipe port 84 of the four-way pipe need to be closed (such as Fig.13 shown);

[0066] Step 5: According to the purification requirements, if the protein adsorbed on the thin layer matrix 25 needs to be eluted, the control valves of the third pipe port 83 of the four-way pipe and the first pipe port 81 of the four-way pipe need to be closed, and the eluent is introduced from the liquid inlet pipe cover 1 to elute the protein adsorbed on the thin layer matrix 25, and the eluent is discharged from the fourth pipe port 84 of the four-way pipe and collected;

[0067] When purification is completed, the inlet pipe cap 1, the chromatography tube half tube A 4, the chromatography tube half tube B 3, the outlet pipe cap 6, the blocking net 20, etc. are disassembled and cleaned, the buffer release pipe port 16 is opened to allow the buffer to flow out of the container, the dialysis bag is removed, and the container is cleaned to prepare for the next protein purification.

[0068] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A protein solution purification device, characterized in that: The invention comprises a chromatography device (15) and a dialysis container (22) connected via a four-way pipe (8), wherein the chromatography device (15) comprises a detachably connected chromatography tube half-tube B (3), a chromatography tube half-tube A (4), and a plurality of blocking nets (20) overlapped between the two, wherein a thin layer matrix (25) is provided on the blocking nets (20); A dialysis device is arranged inside the dialysis container (22), a buffer inlet pipe port (10) and a dialysis effluent pipe (14) are arranged on one side of the dialysis device, and a buffer release pipe port (16) is arranged at the bottom of the dialysis container (22).

2. A protein solution purification device according to claim 1, characterized in that: The chromatography tube half tube B (3) and the chromatography tube half tube A (4) are both arc-shaped plate structures, wherein the inside of the chromatography tube half tube B (3) and the chromatography tube half tube A (4) are provided with a plurality of groups of blocking blocks (19) for overlapping the blocking net (20) along the vertical direction.

3. A protein solution purification device according to claim 2, characterized in that: The two sides of the chromatography tube half tube B (3) are provided with U-shaped slide grooves (26), and the two sides of the chromatography tube half tube A (4) are provided with slide rails (21) corresponding to the U-shaped slide grooves (26), and the U-shaped slide grooves (26) of the chromatography tube half tube B (3) can be slidably connected to the outer sides of the slide rails (21).

4. A protein solution purification device according to claim 1, characterized in that: The chromatography device (15) further comprises a liquid inlet pipe cover (1), a liquid storage pipe (2) and a liquid outlet pipe cover (6), wherein the liquid inlet pipe cover (1) is mounted on the top of the liquid storage pipe (2), the chromatography tube half pipe B (3) and the chromatography tube half pipe A (4) are mounted on the bottom of the liquid storage pipe (2), and the liquid outlet pipe cover (6) is mounted on the bottom of the chromatography tube half pipe B (3) and the chromatography tube half pipe A (4), wherein the liquid outlet pipe cover (6) is connected to the dialysis container (22) via a four-way pipe (8).

5. A protein solution purification device according to claim 1, characterized in that: The chromatography device (15) comprises a dialysis inlet pipe (9) connected to a four-way pipe (8); the dialysis inlet pipe (9) is connected to a dialysis bag (11) via a rubber ring bundle (27); a dialysis bag support net (24) is arranged on the outside of the dialysis bag (11); the dialysis bag (11) is arranged in a circuitous manner, and the other end thereof is connected to a dialysis outlet pipe (14).

6. A protein solution purification device according to claim 5, characterized in that: The dialysis inlet pipe (9) and the dialysis outlet pipe (14) have the same structure. The sides of the dialysis inlet pipe (9) and the dialysis outlet pipe (14) connected to the dialysis bag (11) are both provided with triangular flat interfaces, and the other sides of the dialysis inlet pipe (9) and the dialysis outlet pipe (14) are both cylindrical interfaces.

7. A protein solution purification device according to claim 1, characterized in that: The four-way pipe (8) comprises a first pipe opening (81), a second pipe opening (82), a third pipe opening (83) and a fourth pipe opening (84) of the four-way pipe, wherein the first pipe opening (81) and the third pipe opening (83) of the four-way pipe form a connected horizontal pipe, and the second pipe opening (82) and the fourth pipe opening (84) of the four-way pipe form a connected vertical pipe, wherein the second pipe opening (82) of the four-way pipe is connected to the bottom of the chromatography device (15), and the first pipe opening (81) of the four-way pipe is connected to the dialysis container (22), and a control valve is provided in each pipe opening.

8. A protein solution purification device according to claim 7, characterized in that: The control valves in the third pipe port (83) of the four-way pipe and the fourth pipe port (84) of the four-way pipe are closed, and the protein solution enters the chromatography device (15) and the dialysis container (22) for chromatography and dialysis treatment.

9. A protein solution purification device according to claim 7, characterized in that: The control valves in the second pipe port (82) and the fourth pipe port (84) of the four-way pipe are closed, and a circulation pump is connected between the buffer release pipe port (16) and the third pipe port (83) of the four-way pipe to perform circulating dialysis of the protein solution.

10. A purification method according to any one of claims 1 to 9, characterized in that: The steps include: Close the buffer release port, and pass the buffer (or dialysate) into the dialysis container from the buffer inlet port; Close the four-way tube connecting the chromatography device and the dialysis device, open it, and pass the purified protein solution sample into the chromatography device. After passing through the thin layer matrix and dialysis bag for chromatography and dialysis in turn, the dialysis effluent is collected from the dialysis effluent outlet.

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