Method of using a microfluidic chip

Abstract

The present invention discloses a method for using a microfluidic chip. The microfluidic chip is provided with a sample flow channel, a reagent flow channel, a detection flow channel, a common flow channel, and a mixing chamber. The common flow channel can communicate with the sample flow channel, the reagent flow channel, and the detection flow channel. The mixing chamber is located on the path of the detection flow channel and has an outlet and an inlet. The mixing chamber includes a mixing bend and a mixing chamber. The mixing bend is a curved flow channel and is located on the path of the detection flow channel. The width dimension of the mixing chamber is larger than the diameter dimension of the detection flow channel. The method for using the microfluidic chip includes the steps of: S11, driving the sample in the sample flow channel into the common flow channel; S12, driving the reagent in the reagent flow channel into the common flow channel; S13, driving the sample and the reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, flowing into the mixing chamber after entering the mixing bend from the inlet of the mixing chamber to form a mixed liquid, and then driving the mixed liquid to flow into the detection flow channel again from the outlet of the mixing chamber and flow out from the detection flow channel.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic chips, and particularly relates to a method for using a microfluidic chip. Background Art

[0002] With the increase of human lifespan, the importance of healthcare and maintenance has received more and more attention. Urine can reflect a person's health condition to a certain extent, and diseases of the pancreas and kidneys can be understood through quantitative analysis of urine components. Urine analysis is an important indicator for detecting a person's physical condition, and usually, pH, protein, occult blood, specific gravity, glucose, ketone bodies, urobilinogen, nitrates, white blood cells, bilirubin, and vitamin C can be detected to reflect a series of physical indicators. Usually, urine tests are carried out in hospitals. Due to the shortage of medical resources, after the examinees arrive at the hospital, they often have to queue up for registration, see a doctor, pay fees, collect urine, and wait for the results of batch urine tests and queue up to get the results, which is time-consuming and laborious.

[0003] Currently, urine analyzers for household use have emerged on the market. When the reagent flows in the flow channel plate of the urine analyzer, it cannot be fully mixed and air bubbles will be generated, resulting in unstable test results. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for using a microfluidic chip to solve the problems of the prior art.

[0005] To solve the above technical problems, an embodiment of the present invention provides a method for using a microfluidic chip, and the microfluidic chip is provided with:

[0006] A sample flow channel;

[0007] A reagent flow channel;

[0008] A detection flow channel;

[0009] A common flow channel, which is operably connected to the sample flow channel, the reagent flow channel, and the detection flow channel;

[0010] A mixing chamber, which is located on the path of the detection flow channel and has an outlet and an inlet, and the mixing chamber includes:

[0011] A mixing bend, which is a curved flow channel and is located on the path of the detection flow channel; and

[0012] At least one mixing chamber, the width dimension of which is larger than the diameter dimension of the detection flow channel;

[0013] The method for using the microfluidic chip includes the steps:

[0014] S11. Drive the sample in the sample flow channel into the common flow channel;

[0015] S12. Drive the reagent in the reagent flow channel into the common flow channel;

[0016] S13. Drive the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the mixing bend from the inlet of the mixing chamber and then flow into the mixing chamber to form a mixed liquid, and then drive the mixed liquid to flow into the detection flow channel again from the outlet of the mixing chamber and flow out from the detection flow channel.

[0017] In one embodiment, the mixing chamber is located above the mixing bend, and the inlet of the mixing chamber is below the outlet of the mixing chamber;

[0018] In step S13, drive the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the mixing bend from the inlet of the mixing chamber and then flow into the mixing chamber, and then the mixed liquid flows back into the mixing bend from the mixing chamber, and then drive the mixed liquid to flow into the mixing chamber from the mixing bend, and finally drive the mixed liquid to enter the detection flow channel again from the mixing chamber and flow out from the detection flow channel.

[0019] In one embodiment, the mixing chamber is located above the mixing bend;

[0020] In step S13, drive the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the mixing bend from the inlet of the mixing chamber and then flow into the mixing chamber, and then the mixed liquid flows back into the mixing bend from the mixing chamber, stay for a preset time, and then drive the mixed liquid to flow into the mixing chamber from the mixing bend, and finally drive the mixed liquid to enter the detection flow channel again from the mixing chamber and flow out from the detection flow channel.

[0021] In one embodiment, the mixing chamber includes a plurality of mixing bends and a plurality of mixing chambers, and the plurality of mixing bends and the plurality of mixing chambers are arranged at intervals and communicate with each other;

[0022] In step S13, drive the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the plurality of mixing bends and the plurality of mixing chambers from the inlet of the mixing chamber, and finally drive the mixed liquid to flow out from the detection flow channel.

[0023] In one embodiment, each mixing chamber has an inlet and an outlet respectively, the inlet and the outlet of each mixing chamber communicate with the adjacent mixing bends respectively, and the inlet of each mixing chamber is located above the outlet.

[0024] In step S13, after the sample and the reagent enter the mixing cavity, they flow through one of the mixing bends, pass through an adjacent sampling port, and then enter the mixing cavity. Then, they flow into another mixing bend through the sample outlet. The sample and the reagent continuously flow in a plurality of mixing chambers and a plurality of mixing bends to form the mixed liquid.

[0025] In one embodiment, the mixing bend is an S-shaped bend and includes at least two horizontal flow channels and at least one turning flow channel. Any one of the turning flow channels is located at both ends of the two horizontal flow channels.

[0026] The horizontal flow channel extends in the horizontal direction. The turning flow channel includes a curved inner arc and an outer arc. Both the inner arc and the outer arc are arc-shaped, and the inner diameter of the inner arc is smaller than the outer diameter of the outer arc.

[0027] In one embodiment, the inner wall of the mixing chamber includes a first arc surface and a second arc surface opposite to the first arc surface. The first arc surface and the second arc surface are arc surfaces protruding towards the outside.

[0028] In one embodiment, each mixing chamber communicates with the horizontal flow channels of the two mixing bends, and the two horizontal flow channels are radially symmetrically arranged at the top and bottom of the mixing chamber.

[0029] In step S13, drive the sample and the reagent in the common flow channel to enter the mixing cavity from the detection flow channel. Enter one of the mixing bends from the entrance of the mixing cavity and flow into the mixing chamber, then flow into another mixing bend from the mixing cavity to form the mixed liquid. After staying for a preset time, the mixed liquid flows back into the mixing chamber, and drive the mixed liquid to be discharged from the mixing bend.

[0030] In one embodiment, the microfluidic chip is further provided with a clean water flow channel.

[0031] The common flow channel is operably connected to the clean water flow channel.

[0032] After step S13, it further includes step S14: drive the water in the clean water flow channel to enter the common flow channel, then drive the water in the common flow channel to enter the detection flow channel, then enter the mixing cavity from the entrance of the mixing cavity, and then enter the detection flow channel again from the outlet of the mixing cavity and be discharged from the detection flow channel.

[0033] In one embodiment, the microfluidic chip includes a flow channel plate and a cover plate covering the front side of the flow channel plate.

[0034] The sample flow channel, the reagent flow channel, the detection flow channel, the common flow channel and the mixing cavity are formed by recessing the front side of the flow channel plate;

[0035] The flow channel plate is provided with:

[0036] a central hole that penetrates the flow channel plate and is communicated with the inner end of the common flow channel;

[0037] a plurality of circumferential holes that are arranged circumferentially around the central hole;

[0038] The inner end of the common flow channel is communicated with the central hole, and the outer end of the common flow channel is connected to a pump through a driving pipeline;

[0039] The inner end of the sample flow channel is communicated with one of the circumferential holes;

[0040] The inner end of the reagent flow channel is communicated with one of the circumferential holes;

[0041] The inner end of the detection flow channel is communicated with one of the circumferential holes;

[0042] The central hole is communicated with a transfer channel of a rotor, and the outer end of the transfer channel can be communicated with any one of the circumferential holes as the rotor rotates;

[0043] Step S11: The rotor rotates, the transfer channel is communicated with the sample flow channel through one of the circumferential holes, the pump pumps out the air in the common flow channel and the sample flow channel, and pumps the sample in the sample flow channel into the common flow channel;

[0044] Step S12: The rotor rotates, the transfer channel is communicated with the reagent flow channel through one of the circumferential holes, the pump pumps out the air in the common flow channel and the reagent flow channel, and pumps the sample in the reagent flow channel into the common flow channel;

[0045] Step S13: The rotor rotates, the transfer channel is communicated with the detection flow channel through one of the circumferential holes; the pump drives the sample and reagent in the common flow channel to enter the inlet of the mixing cavity from the detection flow channel, flows into the mixing chamber after entering the mixing cavity from the inlet of the mixing cavity, forms a mixed liquid, and then drives the mixed liquid to flow into the detection flow channel again from the outlet of the mixing cavity and flow out from the detection flow channel.

[0046] In one embodiment, the flow channel plate is provided with two sample flow channels, namely an upper sample flow channel and a lower sample flow channel;

[0047] The inner ends of the lower sample flow channel and the upper sample flow channel are respectively communicated with two of the circumferential holes;

[0048] The lower sample flow channel communicates with the collection cavity for holding samples through a lower sample pipeline, and a burette is provided on the lower sample pipeline;

[0049] The upper sample flow channel communicates with the burette through an upper sample pipeline;

[0050] In step S11, the rotor rotates to align the outer end of the transfer channel with the circumferential hole communicating with the upper sample flow channel, and the pump drives the driving pipeline to extract the air in the common flow channel, the transfer channel, the upper sample flow channel and the burette;

[0051] Then the rotor rotates, aligns the outer end of the transfer channel with the circumferential hole communicating with the lower sample flow channel, and pumps the sample in the collection cavity from the lower sample flow channel to the common flow channel.

[0052] In one embodiment, the flow channel plate further has a clear water flow channel, and the clear water flow channel communicates with one of the circumferential holes;

[0053] After step S13, step S14 is further included: the rotor rotates, the transfer channel communicates with the clear water flow channel through one of the circumferential holes, the pump drives the water in the clear water flow channel to enter the common flow channel, then drives the water in the common flow channel to enter the detection flow channel, then enters the mixing cavity from the inlet of the mixing cavity, and then enters the detection flow channel again from the outlet of the mixing cavity and is discharged from the detection flow channel.

[0054] In one embodiment, the flow channel plate is provided with an upper clear water flow channel and a lower clear water flow channel, and the inner ends of the upper clear water flow channel and the lower clear water flow channel respectively communicate with two of the circumferential holes;

[0055] The lower clear water flow channel communicates with a collection cavity for holding water through a lower clear water pipeline, and a burette is provided on the lower clear water pipeline;

[0056] The upper clear water flow channel communicates with the burette through an upper clear water pipeline;

[0057] After step S13, step S14 is further included: the rotor rotates to align the outer end of the transfer channel with the circumferential hole communicating with the upper clear water flow channel, and the pump pumps the air in the common flow channel, the transfer channel, the upper clear water flow channel and the burette to the outside;

[0058] Then the rotor rotates, aligns the outer end of the transfer channel with the circumferential hole communicating with the lower clear water channel, and pumps the clear water in the collection chamber from the lower clear water channel to the common channel. The rotor rotates, aligns the common channel with the circumferential hole of the detection channel, and discharges the water in the common channel through the detection channel in sequence.

[0059] In one embodiment, the flow channel plate is further provided with an air flow channel, and the inner end of the air flow channel communicates with another circumferential hole;

[0060] In step S13, before the rotor rotates and the transfer channel communicates with the detection channel through one of the circumferential holes; the rotor rotates to align the transfer channel with the circumferential hole communicating with the air, so that the common channel communicates with the air flow channel, and the pump extracts air from the air flow channel into the common channel;

[0061] Then the rotor rotates, and the transfer channel communicates with the detection channel through one of the circumferential holes; the pump drives the sample and reagent in the common channel to enter the inlet of the mixing chamber from the detection channel, flows into the mixing chamber after entering the mixing bend, forms a mixed liquid, and then drives the mixed liquid to flow into the detection channel again from the outlet of the mixing chamber and flow out from the detection channel.

[0062] The microfluidic chip of the present invention is provided with a mixing chamber, which can reduce the bubbles in the liquid, ensure the mixing effect of the sample and the reagent, and increase the accuracy of the test. Description of the Drawings

[0063] Figure 1 is a perspective view of a urine analyzer according to an embodiment of the present invention.

[0064] Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 and Figure 8 are respectively Figure 1 exploded views of the urine analyzer of the embodiment shown.

[0065] Figure 9 is Figures 1 - 8 a perspective view of the locking member in the embodiment shown.

[0066] Figure 10 and Figure 11 are respectively Figure 2 perspective views of the reagent kit in the embodiment shown.

[0067] Figure 12 and Figure 13Respectively are Figure 2 The perspective view of the sealing cover plate in the illustrated embodiment.

[0068] Figure 14 Is Figure 2 The perspective view of the flow channel plate in the illustrated embodiment.

[0069] Figure 15 Is Figure 14 The partial enlarged view of area A of the flow channel plate of

[0070] Figure 16 Is Figure 14 The partial enlarged view of area B of the flow channel plate of

[0071] Figure 17 Is Figure 2 The perspective view of the flow channel plate in the illustrated embodiment.

[0072] Figure 18 Is Figure 4 And Figure 5 The perspective view of the first adapter in the illustrated embodiment.

[0073] Figure 19 Is Figure 4 And Figure 5 The assembly drawing of the kit, flow channel plate, sealing cover plate, first adapter, rotor and protective cover in the illustrated embodiment.

[0074] Figure 20 Is Figure 1 The exploded view of the first housing in the illustrated embodiment.

[0075] Figure 21 Is Figure 2 The perspective view of the detection plate in the illustrated embodiment.

[0076] Figure 22 And Figure 23 Is Figure 2 The exploded view of the protective cover, rotor, compression spring and convex ring in the illustrated embodiment.

[0077] Figure 24 Is Figure 1 The exploded view of the second housing, peristaltic pump, plunger pump and motor in the illustrated embodiment.

[0078] Figure 25 The perspective view of the collector of an embodiment of the present invention.

[0079] Figure 26 And Figure 27 Respectively are Figure 25 The exploded view of the collector in the illustrated embodiment.

[0080] Figure 28 The perspective view of the collector of another embodiment of the present invention.

[0081] Figure 29 and Figure 30 are respectively Figure 28 the exploded views of the collectors of the illustrated embodiments.

[0082] Figure 31 is the flowchart of the control method of the urine analyzer according to an embodiment of the present invention.

[0083] Figure 32 is the liquid path diagram of the urine analyzer according to an embodiment of the present invention. Detailed embodiments

[0084] To make the objectives, technical solutions and advantages of the present invention clearer, the following will elaborate on each embodiment of the present invention in conjunction with the accompanying drawings. However, those of ordinary skill in the art can understand that in each embodiment of the present invention, many technical details are presented for the better understanding of the readers of this application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical solutions claimed in each claim of this application can still be implemented.

[0085] Unless the context requires otherwise, throughout the specification and claims, the words "comprising" and its variants, such as "including" and "having", should be understood in an open, inclusive sense, that is, construed as "including, but not limited to".

[0086] The following will elaborate on each embodiment of the present invention in conjunction with the accompanying drawings to more clearly understand the objectives, features and advantages of the present invention. It should be understood that the embodiments shown in the drawings are not limitations on the scope of the present invention, but only to illustrate the essential spirit of the technical solutions of the present invention.

[0087] References to "an embodiment" or "one embodiment" throughout the specification mean that the particular features, structures or characteristics described in connection with the embodiment are included in at least one embodiment. Thus, appearances of "in an embodiment" or "in one embodiment" throughout the specification do not necessarily all refer to the same embodiment. Additionally, the particular features, structures or characteristics may be combined in any manner in one or more embodiments.

[0088] As used in this specification and the appended claims, the singular forms "a" and "the" include plural referents unless the context clearly dictates otherwise. It should be noted that the term "or" is generally used in its inclusive sense of "and / or" unless the context clearly dictates otherwise.

[0089] In the following description, in order to clearly show the structure and working mode of the present invention, many directional terms will be used for description. However, terms such as "front", "rear", "left", "right", "outer", "inner", "outward", "inward", "up", and "down" should be understood as convenient terms and should not be understood as limiting terms.

[0090] The present invention relates to a urine analyzer 100. As Figures 1 - 4 shown, the urine analyzer 100 includes a housing 1, a collector 4, a reagent kit 2 located inside the housing 1, a rotary cutting valve, a pump, and a detection mechanism. The rotary cutting valve includes a microfluidic chip, a rotor 6, and a driving member 93. Among them, the reagent kit 2 is used to hold a variety of reagents. The collector 4 is provided with a collection chamber, and the collection chamber includes a sample collection chamber 411 and a clean water collection chamber 412. The sample collection chamber 411 is used to collect samples, and the clean water collection chamber 412 is used to store water. The flow channel plate 3 is used to provide a plurality of flow channels for the confluence of reagents and samples. As Figure 5 shown, the detection mechanism includes a detector and a detection plate 5. The detection plate 5 is provided with a detection chamber, and the detection chamber is used to receive the samples and reagents from the flow channel plate 3, and the detection chamber is used to detect the samples in the detection chamber. The rotor 6 is used to cooperate with the flow channel plate 3 to facilitate the collection of samples and reagents and push the samples and reagents into the detection mechanism for detection. Figures 22 - 24 The driving member 93 is connected to the rotor 6 and is used to drive the rotor 6 to rotate.

[0091] Specifically, as Figure 1 shown, the housing 1 includes a first housing 11 and a second housing 12. The rear side of the first housing 11 and the front side of the second housing 12 are detachably connected. The "front side" and "front end" of the urine analyzer 100 are the side of the first housing 11 away from the second housing 12, and the "rear side" or "rear end" is the side of the second housing 12 away from the first housing 11. The reagent kit 2, the rotor 6, and the flow channel plate 3 are located inside the first housing 11, while the pump and the driving member 93 are located inside the second housing 12, and the driving member 93 and the rotor 6 are detachably connected. The driving member 93 and the pump inside the second housing 12 are durable components with a longer service life, while the first housing 11 and the reagent kit 2, the rotor 6, and the flow channel plate 3 inside the first housing 11 are combined into a detection device. This detection device is a consumable item. After using it for a period of time, the reagents in the reagent kit 2 are exhausted, and the detection device can be replaced, which can ensure that things are made the best use of and waste is reduced.

[0092] In a specific embodiment, as Figures 4 - 5As shown, the first housing 11 includes a first front cover 111 and a first rear cover 112. The first front cover 111 and the first rear cover 112 are snap-connected or fixedly connected by bolts, and form a receiving space. As shown, the first front cover 111 includes a first front connecting ring 1111 and a first front cover plate 1112 connected to the front end of the first front connecting ring 1111. The first rear cover 112 includes a first rear connecting ring 1121 and a first rear cover plate 1122 connected to the rear end of the first rear connecting ring 1121. The rear end of the first front connecting ring 1111 and the front end of the first rear connecting ring 1121 face each other and are snap-connected, and are also fixedly connected by bolts. The specific connection method is not limited.

[0093] As Figure 4 shown, the second housing 12 includes a second front cover 121 and a second rear cover 122. The second front cover 121 and the second rear cover 122 are fixedly connected and form another receiving space for receiving the pump and the driving member 93.

[0094] The front end of the second front cover 121 is provided with a recessed mounting groove 1211. As Figure 2 shown, the mounting groove 1211 is used to receive the first rear cover 112.

[0095] In addition, as Figure 8 shown, the urine analyzer 100 further includes two locking members 13. The two locking members 13 are respectively connected to the first rear connecting ring 1121 and are symmetrically arranged on both sides of the first rear connecting ring 1121. The two locking members 13 can detachably connect the first rear cover 112 to the second front cover 121 of the second housing 12.

[0096] Specifically, as Figures 6 - 9 shown, the side wall of the mounting groove 1211 of the second front cover 121 is provided with two locking grooves 1212 arranged at intervals. The two locking members 13 respectively include a locking tongue 131, a rotating member 132 and a handle 133. The rotating member 132 is rotatably connected to the first rear cover plate 1122 of the first rear cover 112 through a rotating shaft. The locking tongue 131 is connected to the rear end of the rotating member 132, and the handle 133 is also connected to the front end of the rotating member 132.

[0097] As Figure 7 shown, the first front connecting ring 1111 is provided with a handle avoidance hole 1113. The handle 133 is plate-shaped and the inner end is connected to the front end of the rotating member 132, and the outer end extends to the outside of the first front connecting ring 1111 through the handle avoidance hole 1113. As Figure 2 、 Figure 6 shown, the first rear connecting ring 1121 is provided with a locking tongue avoidance hole 1123. The inner end of the locking tongue 131 is connected to the rotating member, and the outer end extends into the locking groove 1212 through the locking tongue avoidance hole 1123 and is snap-connected to the locking groove 1212.

[0098] As Figure 2 、 Figure 6 shown, when the first housing 11 needs to be disassembled, by pressing the handle 133 inwardly into the first housing 11, the rotating member 132 rotates, and the locking tongue 131 can be moved out of the locking groove 1212. During installation, by rotating the handle 133 in the reverse direction, the locking tongue 131 can be locked into the locking groove 1212.

[0099] In addition, two limiting members 1124 are further provided on the first rear connection ring 1121. As Figure 7 shown, the two limiting members 1124 are located at both ends of the handle avoidance hole 1113, and two bumps 134 are further provided at the inner end of the handle 133. The two bumps 134 are located inside the first rear connection ring 1121 and are blocked by the two limiting members 1124 to prevent the handle 133 from detaching from the first rear connection ring 1121.

[0100] In other embodiments, an elastic member may be provided between the rotating member 132 and the inner wall of the first rear connection ring 1121. The elastic member can pull the locking tongue 131 of the rotating member 132 to move towards the locking groove 1212 to prevent the locking tongue 131 from automatically detaching from the locking groove 1212. Of course, in other embodiments, the locking tongue 131 and the locking groove 1212 are in interference fit, and the locking tongue 131 can also be locked tightly.

[0101] It should be understood that in other embodiments, the first housing 11 and the second housing 12 may also be snap-connected, magnetically attracted, or bolt-connected. The present invention does not limit the specific connection manner between the first housing 11 and the second housing 12.

[0102] The reagent kit 2 is installed in the first housing 11 and is provided with a plurality of reagent chambers 21 for containing reagents. As Figures 10 - 11 shown, the reagent kit 2 is in the shape of a flat plate and is provided with a plurality of reagent chambers 21 and a plurality of flow channel holes 22. The plurality of reagent chambers 21 extend in the vertical direction and are arranged in the first direction, and the first direction is the width direction of the urine analyzer 100. The reagent kit 2 is in the shape of a plate and is stacked with the flow channel plate 3. The outer periphery of the reagent kit 2 basically fits the inner wall of the first housing 11. The plurality of reagent chambers 21 basically occupy the entire reagent kit 2, can contain more reagents, and do not occupy too much space.

[0103] As Figure 6 、 Figure 10 shown, both the first front connection ring 1111 and the first rear connection ring 1121 of the first housing 11 are annular, that is, the inner side wall of the first housing 11 is an arc surface, the bottom end of the reagent kit 2 also presents an arc shape, and the bottom ends of the plurality of reagent chambers 21 are arranged around a horizontal axis and are basically on the same circumference. The bottom ends of the plurality of reagent chambers 21 are close to the bottom end of the reagent kit 2 to increase the utilization rate of the reagent chambers 21.

[0104] As shown Figure 10 As shown, the bottom wall of the reagent chamber 21 includes an inclined surface 23 and a vertical surface 24. The vertical surface 24 extends in the vertical direction, while the inclined surface 23 is an inclined surface that gradually decreases from the rear to the front. The bottom end of the inclined surface 23 is connected to the top surface of the vertical surface 24.

[0105] The top end and the front side of the reagent chamber 21 are open. Reagents can be put in from the top end, and then a seal can be set at the top end of the reagent chamber 21, or an oil seal can be used. A sealing plate (not shown in the figure) is also installed at the front end of the reagent kit 2. This sealing plate is stacked on the front side of the reagent kit 2 and can seal the reagent chamber 21. Of course, when processing is convenient, the sealing plate can also not be provided, and the front side of the sealing chamber can be set in a sealed shape.

[0106] As shown Figure 10 and Figure 11 As shown, a plurality of flow channel holes 22 are also arranged at intervals around the horizontal axis and recess from the rear side of the reagent kit 2 towards the front end. The plurality of flow channel holes 22 recess to the vertical surface 24 and communicate with the plurality of reagent chambers 21. The inclined surface 23 is provided to facilitate the slow flow of the reagent to the bottom end and flow into the flow channel plate 3 through the flow channel holes 22.

[0107] As shown Figure 10 and Figure 11 As shown, a plurality of sealing grooves 25 are also provided on the rear side of the reagent kit 2. The plurality of sealing grooves 25 are formed by recessing from the rear side of the reagent kit 2, and the plurality of flow channel holes 22 are formed by recessing from the inner wall of the plurality of sealing grooves 25 away from the second housing 12 to the vertical surface 24. The sealing grooves 25 can also be set in a conical shape, that is, the sealing grooves 25 gradually decrease from the rear to the front, and the external opening is larger.

[0108] As shown Figure 7 , Figure 12 As shown, the microfluidic chip includes a flow channel plate 3 and a cover plate 7 stacked on the front side of the flow channel plate 3. Among them, the cover plate 7 is located on the rear side of the reagent kit 2. The cover plate 7 is in a thin plate shape and is stacked on the rear side of the reagent kit 2 and the front side of the flow channel plate 3. A plurality of conical insertion columns 71 are provided on the front side of the cover plate 7. The plurality of insertion columns 71 are respectively located in the plurality of sealing grooves 25, and a plurality of sealing rings are sleeved outside the plurality of insertion columns 71, so that the plurality of insertion columns 71 and the plurality of sealing grooves 25 are sealed and connected.

[0109] As shown Figure 7 , Figure 12 As shown, a plurality of through holes 72 are also provided on the rear side of the cover plate 7. The plurality of through holes 72 extend to the front ends of the plurality of insertion columns and are respectively communicated with the plurality of flow channel holes 22. The reagent in the reagent chamber 21 can flow into the flow channel plate 3 through the flow channel holes 22 and the through holes 72 of the insertion columns 71.

[0110] The cover plate 7 and the kit 2 are snap - connected, bonded, or connected by a thermoplastic method. The present invention does not limit the specific connection method between the cover plate 7 and the kit 2.

[0111] In Figures 11 - 13 the illustrated embodiment, a clamping groove 26 is provided at the bottom end of the kit 2, and a protruding buckle 73 is provided at the bottom end of the cover plate 7. The buckle 73 can be snapped into the clamping groove 26 of the kit 2, so that the kit 2 and the cover plate 7 can be quickly assembled.

[0112] The flow - channel plate 3 is stacked on the rear side of the cover plate 7. The flow - channel plate 3, the cover plate 7, and the kit 2 are fixedly connected to the first front cover plate 1112 or the first rear cover plate 1122 of the first housing 11 by the same bolt. In Figure 4 and Figure 7 the illustrated embodiment, two mounting posts 14 are respectively provided on the inner walls of the first front cover plate 1112 and the first rear cover plate 1122 of the first housing 11, and the mounting posts 14 of the first front cover plate 1112 and the first rear cover plate 1122 are abutted against each other and are both provided with threaded holes. The threaded hole of the mounting post 14 of the first rear cover plate 1122 extends to the rear side of the first rear cover plate 1122. The bolt can be screwed into the threaded hole of the mounting post 14 of the first front cover plate 1112 from the rear side of the first rear cover plate 1122, so as to fix the first rear cover plate 1122 and the first front cover plate 1112.

[0113] In addition, as Figure 7 shown, the kit 2 is provided with a countersunk hole 27, and the first rear cover plate 1122 is further provided with another mounting post 14. The threaded hole of the mounting post 14 and the countersunk hole 27 are arranged oppositely, and a flange is provided at the inner end of the mounting post 14. A part of the flange can abut against the rear side of the flow - channel plate 3. Screwing the bolt into the countersunk hole 27 from the rear side of the first rear cover plate 1122 can fix the kit 2, the cover plate 7, and the flow - channel plate 3 together on the first rear cover plate 1122, and make the kit 2, the cover plate 7, and the flow - channel plate 3 fit tightly.

[0114] The front side of the flow - channel plate 3 is provided with a central hole 31, a plurality of circumferential holes 32, a common flow - channel 33, a sample flow - channel, a clean - water flow - channel, an air flow - channel 36, a plurality of reagent flow - channels 37, and a detection flow - channel 38. As Figures 14 - 16 shown, the central hole 31 is located on the above - mentioned horizontal axis, and the central hole 31 is a through - hole 72 that penetrates the flow - channel plate 3 from front to back. The plurality of circumferential holes 32 are located on a circumference centered on the central hole 31 and are arranged at intervals, that is, the plurality of circumferential holes 32 are on the same circumference.

[0115] The inner ends of the sample flow channel, the clear water flow channel, the inner end of the air flow channel 36, the inner end of the detection flow channel 38, and the inner ends of the plurality of reagent flow channels 37 respectively extend to the plurality of circumferential holes 32 and communicate with the plurality of circumferential holes 32. The outer ends of the sample flow channel, the clear water flow channel, the outer end of the air flow channel 36, the outer end of the detection flow channel 38, and the outer ends of the plurality of reagent flow channels 37 are respectively dispersed away from the central hole 31 to other positions of the flow channel plate 3. Among them, the outer end of the detection flow channel 38 communicates with the detection cavity of the detection mechanism, facilitating the sample and reagent to flow into the detection cavity from the detection flow channel 38. The outer ends of the plurality of reagent flow channels 37 respectively extend to another circumference and align and communicate with the plurality of through holes 72 of the plurality of cover plates 7.

[0116] In some embodiments, one sample flow channel and one clear water flow channel are respectively provided. The outer end of the clear water flow channel is connected to the clear water collection cavity 412 of the collection cavity through a pipeline, facilitating water to enter the clear water flow channel from the clear water collection cavity 412. The sample flow channel is connected to the sample collection cavity 411 through a pipeline, facilitating the sample to enter the sample flow channel from the sample collection cavity 411.

[0117] In Figure 14 and 16 In the illustrated embodiment, two clear water flow channels and two sample flow channels are respectively provided. The inner ends of the two clear water flow channels and the two sample flow channels are respectively connected to different circumferential holes 32. The usage methods of the two clear water flow channels and the two sample flow channels will be described in detail below.

[0118] To facilitate the connection of the sample flow channel, the detection flow channel 38, the reagent flow channel 37, and the air flow channel 36 with the sample collection cavity 411, the detection cavity, and the reagent cavity 21, a first adapter 301 is further installed at the rear side of the flow channel plate 3. The first adapter 301 can be installed in the first housing 11 or in the second housing 12.

[0119] Specifically, as Figure 18 and Figure 19 shown, if the first adapter 301 is installed in the first housing 11, the first adapter 301 is snap-connected or fixedly connected to the rear side of the flow channel plate 3 through bolts, or welded, etc. The specific connection method between the first adapter 301 and the rear side of the flow channel plate 3 is not limited.

[0120] The first adapter 301 is used to introduce the sample and clear water into the microfluidic chip, and also discharge the waste liquid after detection in the detection plate into the collector, and then from the collector to the toilet. Moreover, after the reagent is used up, the first adapter 301 and the pipeline are disassembled together, and all the components in the new first housing are directly replaced, reducing pollution and increasing the service life of the urine analyzer.

[0121] As Figure 18 , Figure 26 , Figure 27As shown, the first adapter 301 is provided with seven transfer pipes. The seven transfer pipes are used to communicate with the sample collection chamber 411, the clean water collection chamber 412, the sewage discharge channel 416 of the detector, or the external air through multiple pipes. The transfer pipes are preferably arranged at the top of the first adapter 301, which is convenient for samples or water to flow from the sample collection chamber 411 or the clean water collection chamber 412 into the flow channel plate 3, and is also convenient for the liquid in the detection chamber to flow into the sewage discharge channel 416 of the collector through the connecting pipe and the pipe.

[0122] As Figure 18 , Figure 26 , Figure 27 As shown, among the seven transfer pipes, two of the transfer pipes communicate with the sample collection chamber 411 of the collector, and this transfer pipe is defined as the sample transfer pipe 3011. Another two transfer pipes communicate with the clean water collection chamber 412 of the collector, and these two transfer pipes are defined as the clean water transfer pipes 3012. Another transfer pipe communicates with the air flow channel 36 of the flow channel plate 3, and is defined as the air transfer pipe 3013. Another transfer pipe communicates with the sewage discharge channel 416 of the collector, and is defined as the sewage transfer pipe 3014. The last transfer pipe communicates with the drive pipe, and is defined as the drive transfer pipe 3015.

[0123] As Figure 18 As shown, seven first slots 3016 are provided on the front side of the first adapter 301. There are seven channels in the first adapter 301. The multiple channels extend forward and communicate with the seven transfer pipes. The bottom ends of the seven channels communicate with the seven first slots 3016.

[0124] As Figure 14 , Figure 17 As shown, nine first adapter posts 3017 are further provided on the rear side of the flow channel plate 3. The nine first adapter posts 3017 are respectively provided with first adapter holes. The nine first adapter holes respectively extend into the flow channel plate 3 and communicate with the clean water flow channel, the detection flow channel 38, the sample flow channel, or the air flow channel 36 of the flow channel plate 3. Seven of the nine first adapter posts 3017 are respectively inserted into the seven first slots 3016 of the first adapter. The other two need to be inserted into the inlets and outlets of the detection plate. Among the seven first adapter posts, the first adapter holes of two of the first adapter posts 3017 communicate with the two sample flow channels of the flow channel plate 3, and the first adapter holes of these two first adapter posts 3017 also communicate with the two sample transfer pipes 3011. The first adapter holes of another two first adapter posts 3017 communicate with the two clean water flow channels and also communicate with the two clean water transfer pipes 3012.

[0125] As Figure 14 , Figure 17 , Figure 18As shown, the first transfer hole of one of the first transfer posts 3017 communicates with the air flow channel 36 and the air transfer pipe 3013. The first transfer hole of the other first transfer post 3017 communicates with the drive pipe and the drive transfer pipe 3015. There are also three first transfer holes of the first transfer posts 3017, one of which is for communicating with the sewage transfer pipe 3014, and the other two are for communicating with the detection chambers respectively, which will be described in detail below.

[0126] In summary, the seven first transfer posts 3017 are respectively inserted into the seven first slots 3016 of the first transfer member, so that the outer end of the sample flow channel of the flow channel plate 3 communicates with the sample transfer pipe 3011, the outer end of the clear water flow channel communicates with the clear water transfer pipe 3012, the outer end of the air flow channel 36 communicates with the air transfer pipe 3013, and the outer end of the detection flow channel 38 communicates with the sewage transfer pipe 3014.

[0127] In another embodiment, the first transfer member 301 is installed in the second housing 12 or can also be installed in the installation groove. Since the second housing 12 and the first housing 11 are detachably connected, regardless of whether the first transfer member 301 is connected inside or outside the second housing 12, the multiple first slots 3016 of the first transfer member 301 need to be exposed in the installation groove 1211 of the second housing 12.

[0128] As Figure 17 shown, there are also multiple first transfer posts 3017 provided on the rear side of the flow channel plate 3, and the first rear cover plate 1122 of the first housing 11 also needs to be provided with avoidance holes for avoiding the multiple first transfer posts 3017, so as to facilitate the insertion of the first transfer posts 3017 into the first slots 3016 of the first transfer member 301 as the first housing 11 and the second housing 12 are assembled.

[0129] As Figure 6 、 Figure 17 、 Figure 18 shown, when the first housing 11 and the second housing 12 are assembled, when the locking tongue 131 of the locking member 13 penetrates into the locking groove 1212, the multiple first transfer posts 3017 can be simultaneously pressed into the multiple first slots 3016 respectively.

[0130] Of course, in order to increase the sealing performance between the first transfer member 301 and the flow channel plate 3, a sealing ring sleeved outside the first transfer post 3017 also needs to be provided in the first slot 3016.

[0131] As Figure 18 、 Figure 19 shown, the detection plate 5 can be installed in the first housing 11 and fixedly connected or snapped to the first transfer member 301, or fixedly connected to the rear side of the flow channel plate 3. Of course, in other embodiments, the detection plate 5 can also be arranged in the second housing 12 and be configured to be detachably connected to the first transfer member 301.

[0132] Figure 14 and 19 As shown in 19 , if disposed within the first housing 11, the detection plate 5 can be directly fixedly connected to the rear side of the flow channel plate 3. One end of the detection chamber is communicated with the detection flow channel 38, and the other end is communicated with the sewage transfer pipe 3014 of the first adapter 301. The sewage transfer pipe 3014 is communicated with the sewage discharge channel 416 of the collector through a pipeline. The liquid in the detection flow channel 38 flows into the detection chamber for detection. After the detection is completed, the liquid in the detection chamber can flow from the sewage transfer pipe 3014 into the sewage discharge channel 416 and be discharged into the toilet from the sewage discharge channel 416.

[0133] Specifically, as Figure 5 and Figure 19 and Figure 21 shown, the detection plate 5 is a plate-shaped one extending forward and backward along the urine analyzer 100. The detection chamber is generally U-shaped and has an inlet 51 and an outlet 52, and the inlet 51 and the outlet 52 are respectively located at the front end of the detection plate 5. As Figure 17 shown, there are three first adapter posts 3017 on the rear side of the flow channel plate 3. Two of the first adapter posts 3017 are respectively inserted into the inlet 51 and the outlet 52. The first adapter post 3017 inserted into the inlet 51 communicates the detection flow channel 38 of the flow channel plate 3 with the inlet 51. The samples and liquids in the detection flow channel 38 can sequentially flow into the detection chamber from the inlet 51 for detection. That is to say, one end of the detection chamber is communicated with the detection flow channel, and the other end is communicated with the sewage pipeline.

[0134] As Figure 14 and Figure 17 and Figure 18 shown, the flow channel plate 3 is further provided with a sewage flow channel 302. Both ends of the sewage flow channel 302 are respectively communicated with the other two first adapter posts 3017 on the rear side of the flow channel plate 3. One of the first adapter posts 3017 of the two first adapter posts 3017 is inserted into the outlet 52 of the detection chamber, and the first adapter hole of the first adapter post 3017 is communicated with the outlet 52. The other first adapter post 3017 is inserted into the first slot 3016 at the front end of the first adapter 301 and is communicated with the sewage transfer pipe 3014 located at the top of the first adapter 301.

[0135] That is to say, the mixed liquid of the samples and reagents in the detection flow channel 38 flows into the detection chamber from the first adapter hole of one of the first adapter posts 3017 and the inlet 51 of the detection chamber. After the detection is completed, the mixed liquid then flows from the outlet 52 into the sewage flow channel 302 of the flow channel plate 3, and then flows from the sewage flow channel 302 into the sewage transfer pipe 3014, and flows into the sewage discharge channel 416 of the collection chamber from the sewage transfer pipe 3014 and the pipeline, and then flows into the toilet from the sewage discharge channel 416.

[0136] The function of the sewage flow channel 302 is equivalent to a transfer flow channel. One end is connected to the flow outlet 52 of the detection cavity, and the other end is connected to the sewage transfer connection pipe 3014. The mixed liquid in the detection cavity passes through the sewage flow channel 302 and then flows into the sewage channel 416 through the sewage transfer connection pipe 3014. This design can reduce the volume of the first adapter 301 and make full use of the internal space of the flow channel plate 3 and the first adapter 301.

[0137] If the detection plate 5 is installed in the first housing 11, when the reagent in the first housing 11 is exhausted, it can be disassembled along with the first housing 11. When replacing the new detection device, a new detection plate 5 is also installed inside it.

[0138] The detector of the present invention includes a light source device and a photosensitive sensor. The light source device and the photosensitive sensor are respectively arranged at corresponding positions of the detection plate 5. The detection plate 5 is made of a transparent material. The light source device is an LED light, which is used to irradiate the mixed liquid in the detection cavity, and the photosensitive sensor is used to sense the light after the light source device irradiates the mixed liquid to detect the sample components. However, the installation of the detector is relatively complex. Therefore, for the convenience of customers' assembly, the detector needs to be installed in the second housing 12, that is, the detector and the detection plate 5 are assembled separately. And the detector needs to be close to the detection plate 5 to detect the mixed liquid in the detection cavity. Therefore, the rear end of the detection plate 5 needs to extend into the second housing 12.

[0139] Specifically, as Figure 20 shown, avoiding holes need to be provided on the inner walls of the first rear cover plate 1122 of the first housing 11 and the installation groove 1211 of the second housing 12 respectively. This avoiding hole is the detection plate avoiding hole 15. The front end of the detection plate 5 is connected to the flow channel plate 3 and the first adapter 301, and the rear end extends into the second housing 12 from the two detection plate avoiding holes 15 of the first housing 11 and the second housing 12. The detector is installed at the top or bottom of the detection plate avoiding hole 15 of the second housing 12, and can detect the liquid in the detection cavity.

[0140] In another embodiment, the detection plate 5 can also be directly installed in the second housing 12. The front end of the detection plate 5 extends to the inner wall of the installation groove 1211. Among the three first adapter posts 3017 at the rear end of the flow channel plate 3, the first adapter holes of two of the first adapter posts 3017 are respectively communicated with both ends of the sewage flow channel 302. The first adapter hole of one of the two first adapter posts 3017 is communicated with the sewage transfer connection pipe 3014, and the other is communicated with the flow outlet 52 of the detection cavity.

[0141] Among the three first adapter posts 3017, the first adapter hole of the other first adapter post 3017 is communicated with the flow inlet 51 of the detection cavity.

[0142] The two first adapter columns 3017 connected to the inlet 51 and the outlet 52 of the detection chamber need to extend to the rear side of the first rear cover plate 1122 of the first housing 11. When the first housing 11 and the second housing 12 are assembled, the rear ends of the two first adapters 301 can be inserted into the two inlets 51 and the outlet 52 at the front end of the detection plate 5, and quick disassembly and assembly can also be achieved.

[0143] It should be understood that a sealing connection is required between the multiple transfer pipes and the multiple first adapter columns 3017, or between the first adapter column and the outlet 52 and the inlet 51. The sealing method can be selected as an O-ring or other methods.

[0144] The common flow channel 33 is a curved flow channel, and its inner end extends to the central hole 31 and is connected to the central hole 31. The outer end bends around the central hole 31 within the circle formed by the multiple circumferential holes 32 and then bends and extends to the outside of the flow channel plate 3. The common flow channel 33 and the multiple circumferential holes 32 are arranged in a staggered manner and are not directly connected to each other.

[0145] In one embodiment, the outer end of the common flow channel 33 is used to communicate with the drive pipe, and the drive pipe is located within the second housing and is connected to the pump. The pump can extract the air within the drive pipe and the common flow channel 33. Of course, in some embodiments, two pumps can be arranged in series through a pipe, and one of the pumps is connected to the drive pipe.

[0146] In Figure 14 In the illustrated embodiment, at least one bend is provided in the middle part of the common flow channel 33. On the one hand, it increases the volume of the common flow channel 33, and on the other hand, it prevents the reagent or sample within the common flow channel 33 from flowing back into the drive pipe.

[0147] The common flow channel 33 needs to be connected to the drive pipe, and the pump is connected to the drive pipe. After the pump is started, the air within the common flow channel 33 can be emptied to form a negative pressure.

[0148] The pump and the drive pipe are respectively located within the second housing 12. Therefore, in order to achieve quick disassembly and assembly of the drive pipe and the common flow channel 33, a second adapter 306 also needs to be installed. The second adapter 306 will be introduced below.

[0149] As a preferred solution, in order to more accurately and quantitatively extract the reagent and the sample, two pumps can be used to communicate with the common flow channel through two drive pipes respectively. One is a peristaltic pump 91, and the other is a plunger pump 92. The two pumps are respectively installed within the second housing 12 and are respectively connected to the two drive pipes. The two drive pipes are respectively defined as the first drive pipe and the second drive pipe.

[0150] The second driving pipeline is connected to the plunger pump, and the first driving pipeline is connected to the peristaltic pump 91. The first driving pipeline includes two sections, and the two ends of the two sections are respectively connected to the two ends of the hose of the peristaltic pump, and the other two sections are respectively connected to the two peristaltic pump adapter posts 3041 described below.

[0151] On the front side of the flow channel plate 3, there is also a driving flow channel 303. The driving flow channel 303 extends in the vertical direction and its top end communicates with the first transfer hole of one of the first transfer posts 3017. The first transfer hole communicates with the driving transfer pipe 3015.

[0152] On the rear side of the flow channel plate 3, there are also three second transfer posts. The three second transfer posts are respectively provided with second transfer holes. Among the three second transfer posts, two of them are peristaltic pump adapter posts 3041, and the other one is a plunger pump adapter post 3042.

[0153] Such as Figure 14 、 Figure 17 As shown, the second transfer hole of one of the two peristaltic pump adapter posts 3041 communicates with the bottom end of the driving flow channel 303, and the second transfer hole of the other peristaltic pump adapter post 3041 communicates with the common flow channel 33.

[0154] The plunger pump adapter post 3042 is located between the two peristaltic pump adapter posts 3041 and its second transfer hole also communicates with the common flow channel 33.

[0155] Figure 17 、 Figure 18 As shown, the second adapter 306 is connected to the inner wall of the installation groove 1211 of the second housing 12, and is provided with three second slots 3061. Among the three second slots 3061, two of the second slots 3061 respectively accommodate the two peristaltic pump adapter posts 3041. That is, the common flow channel 33 communicates with one end of one section of the first driving pipeline through one peristaltic pump adapter post 3041, and one end of the other section of the first driving pipeline communicates with the bottom end of the driving flow channel 303 through the other peristaltic pump adapter post 3041. The top end of the driving flow channel 303 is connected to the driving transfer pipe 3015 through the first connecting post, and the driving transfer pipe 3015 can also be connected to the collector. That is to say, one end of the two sections of the first driving pipeline is respectively connected to the two ends of the hose of the peristaltic pump, and the other end is respectively connected to the common flow channel and the driving transfer pipe 3015. After the peristaltic pump is started, the fluid in the common flow channel can be pumped from the first driving pipeline and the driving transfer pipe to the collector and discharged from the collector into the toilet.

[0156] Among the three second slots 3061, the other second slot 3061 communicates with one end of the second driving pipeline, and the second slot 3061 accommodates the plunger pump adapter post 3042.

[0157] The peristaltic pump 91 is used to drive the air discharge of the common flow channel 33, or drive the samples and water in the sample collection chamber into the sample flow channel and the clean water flow channel. That is to say, a relatively large air flow path is required. Therefore, a driving flow channel 303 is provided on the flow channel plate 3, and a driving adapter pipe 3015 is provided on the first adapter 301. If it is bent, a relatively large air flow path can be provided. The plunger pump 92 only needs to quantitatively extract the samples and reagents in the sample flow channel and the reagent flow channel 37 into the common flow channel, and the required flow path is relatively small. Therefore, one end of the second driving pipe is connected to the common flow channel 33, and the other end is arranged near the plunger pump 92.

[0158] As Figure 5 shown, the detection flow channel 38 is connected to the detection chamber, and a mixing chamber 39 is also provided on the path of the detection flow channel 38. The mixing chamber 39 is connected to the detection flow channel 38 and the width of the mixing chamber 39 is greater than the diameter of the detection flow channel 38. When the sample and the reagent flow in the detection flow channel 38 and pass through the mixing chamber 39, due to the relatively large width of the mixing chamber 39, the sample and the reagent will accelerate when flowing from the detection flow channel 38 into the mixing chamber 39, and then be mixed in the mixing chamber 39.

[0159] As a preferred solution, the mixing chamber 39 includes a mixing bend 392 and at least one mixing chamber 391 that are connected to each other. The mixing bend 392 is an S-shaped curved flow channel, and the mixing chamber 391 is connected to the mixing bend 392 and the width dimension of the mixing chamber 39 is greater than the diameters of the mixing bend 392 and the detection flow channel 38.

[0160] In Figures 14 - 16 the embodiment shown, the mixing chamber 39 includes three mixing bends 392 and three mixing chambers 391. The three mixing chambers 391 and the three mixing bends 392 are arranged alternately and connected to each other, and the inlet of the mixing chamber 39 is at the bottom end and the outlet is at the top end. That is to say, the three mixing bends 392 and the mixing chamber 39 extend upward in a curved shape.

[0161] The mixing bend 392 includes at least two horizontal flow channels and at least one turning flow channel 394, and any one of the turning flow channels 394 is located at both ends of the two horizontal flow channels;

[0162] The mixing bend 392 is an S-shaped bend and includes at least two horizontal flow channels and at least one turning flow channel 394. The horizontal flow channel is a flow channel extending in the horizontal direction, and the turning flow channel 394 is located between any two horizontal flow channels, that is, at the corner of the mixing bend 392.

[0163] As Figure 16 shown, the turning flow channel 394 includes a curved inner arc 395 and an outer arc 396. Both the inner arc 395 and the outer arc 396 are arcs and the inner diameter of the inner arc 395 is smaller than the outer diameter of the outer arc 396.

[0164] The mixing chamber 39 of the present invention adopts an S-shaped mixing bend 392 in cooperation with the mixing chamber 39, and the outer diameter is enlarged at the turning corner of the mixing bend 392. By using the bend with variable diameter here, the flow velocity at the outer arc 396 is greater than that at the inner arc 395, forming a local Dean flow effect, and improving the mixing effect of the mixed liquid of the sample and the reagent in the flow channel.

[0165] As shown in Figure 15 , the vertical section of the mixing chamber 391 is approximately circular, and the two side walls of the mixing chamber 391 are also arc surfaces, which are respectively a first arc surface 397 and a second arc surface 398 opposite to the first arc surface 397. Both the first arc surface 397 and the second arc surface 398 are arc surfaces protruding towards the outside.

[0166] As a preferred solution, as shown in Figure 15 , the first arc surface 397 and the second arc surface 398 are circular arc surfaces with the same diameter and the same arc length, and the tangents at both ends of the first arc surface 397 and the second arc surface 398 are parallel to each other. In the embodiment shown in the figure, the arc length of the first arc surface 397 is equal to 1 / 4 of the circumference of the circle, and the tangents at both ends are perpendicular to each other, and one of the tangents extends in the vertical direction and the other extends in the horizontal direction.

[0167] Since the entire mixing chamber 39 extends in a bent manner in the vertical direction, as shown in Figure 16 , each mixing chamber 391 is located between two mixing bends 392. Each mixing chamber 39 needs to be connected to the horizontal flow channels of the two mixing bends 392. The two horizontal flow channels are symmetrically arranged in a mirror image. One is located at the top of the mixing chamber 391, and the other is located at the bottom of the mixing chamber 391.

[0168] Among the two horizontal flow channels connected to the mixing chamber 391, one horizontal flow channel is defined as the liquid inlet horizontal flow channel 3931, and the other horizontal flow channel is defined as the liquid outlet horizontal flow channel 3932. The liquid inlet horizontal flow channel 3931 is connected to the top of the mixing chamber 391, and the liquid outlet horizontal flow channel 3932 is connected to the bottom of the mixing chamber 391. The sample or reagent flows into the mixing chamber 391 from the liquid inlet horizontal flow channel 3931 and then flows out from the liquid outlet horizontal flow channel 3932. That is to say, the liquid inlet horizontal flow channel 3931 is located upstream of the liquid outlet horizontal flow channel 3932. The sample and the reagent flow from the common flow channel 33 into the detection flow channel 38. After passing through, for example, the mixing chamber 39, they enter the mixing chamber 391 from the liquid inlet horizontal flow channel 3931 of one mixing bend 392 and then flow into the horizontal flow channel of another mixing bend 392.

[0169] As shown in Figure 15As shown, the mixing chamber 391 has a sample inlet 3911 and a sample outlet 3912. Among them, the sample inlet 3911 is connected to the liquid inlet horizontal flow channel of one of the mixing bends, and the sample outlet 3912 is connected to the liquid outlet horizontal flow channel 3932 of the other mixing bend. The sample and the reagent can flow into the mixing chamber from the liquid inlet horizontal flow channel of one of the mixing bends and the sample inlet 3911, and then flow into the other mixing bend from the sample outlet 3912 and the liquid outlet horizontal flow channel, and continuously flow in several mixing chambers and several mixing bends.

[0170] Taking the connection line between the liquid inlet horizontal flow channel 3931 and the liquid outlet horizontal flow channel 3932 as the dividing line, as Figure 15 shown by the dotted line. This dividing line can divide the mixing chamber 391 into two parts. Among them, the area formed by this dividing line and the first arc surface 397 located below is the slow zone 3934, and the area between the dividing line and the second arc surface 398 is the fast zone 3933. The flow rate of the flow entering the fast zone 3933 and the slow zone 3934 of the mixing chamber 391 from the liquid inlet horizontal flow channel 3931 has a certain speed difference. The mixed liquid of the sample and the reagent forms a vortex around the center point in the circular mixing chamber 391, increasing the contact area between the liquids and improving the mixing effect.

[0171] Due to the narrowing of the liquid outlet horizontal flow channel 3932, when the mixed liquid of the sample and the reagent flows out from the liquid outlet horizontal flow channel 3932, the speed suddenly increases, and the mixed liquid of the sample and the reagent is relatively squeezed, further increasing the mixing intensity of the mixed liquid of the sample and the reagent. Through the series connection of the above-mentioned mixing bends 392 with multiple diameter changes and the circular mixing chamber 391, the mixing intensity is gradually increased until the detection chamber, and the sample and the reagent are basically in a completely mixed state.

[0172] This mixing chamber 39 of the present invention is often used in the technical fields of microfluidic chips such as biology and chemistry. When sample or reagent or the mixture of sample and reagent is required, using this structure can greatly improve the mixing efficiency and avoid some problems of residue and bubbles at the same time.

[0173] In this mixing chamber 39, the liquid inlet horizontal flow channel 3931 connected to each mixing chamber 391 is relatively higher than the liquid outlet horizontal flow channel 3932. In special application scenarios, such as when it needs to be used vertically, when the liquid flows from bottom to top, the influence of gravity does not need to be considered, so that there will be no residue problem in the mixing chamber 391.

[0174] When the sample or reagent flows in a relatively rough flow channel, bubbles are easily generated, which will affect the mixing effect and even be brought into the detection chamber, causing analysis errors. In this application, multiple mixing chambers 39 of the chip are connected in series with the mixing bends 392, and smooth fillet transitions are made at the flow channel corners of each mixing bend 392, which can avoid and eliminate bubbles in the mixing chamber 391 to a certain extent.

[0175] The inlet of the mixing chamber 39 is located below the outlet. The mixing chamber 39 is located above the common flow channel 33. The inlet of the mixing chamber 39 is in communication with the common flow channel 33, and the outlet is in communication with the detection chamber. After the sample and reagent in the common flow channel 33 flow into the mixing chamber 39 from the bottom inlet of the mixing chamber 39, they then flow into the detection chamber from the outlet.

[0176] As Figure 14 、 Figure 22 shown, the rotor 6 is rotatably installed inside the first housing 11 and is located on the rear side of the flow channel plate 3. There is a transfer channel inside the rotor 6. The inner end 61 and the outer end of this transfer channel open towards the front side of the rotor 6. The transfer channel is generally U-shaped. The inner end 61 of the transfer channel is aligned with the central hole 31, and the inner end is recessed from the front side of the rotor 6 into the interior of the rotor 6. The outer end 62 of the transfer channel also opens towards the front side of the rotor 6, and the distance from the outer end 62 to the inner end 61 is the same as the distance between the central hole 31 and the plurality of circumferential holes 32.

[0177] As Figure 14 、 Figure 22 shown, the inner end 61 of the transfer channel is aligned and in communication with the central hole 31. The outer end 62 rotates with the rotor 6 and can be aligned and in communication with any one of the circumferential holes 32 as the rotor 6 rotates.

[0178] When it is necessary to extract a sample, the rotor 6 rotates so that the outer end 62 of the transfer channel is aligned with the circumferential hole 32 that is in communication with the inner end of the sample flow channel. At this time, both ends of the transfer channel are in communication with the sample flow channel and the common flow channel 33 respectively. The peristaltic pump 91 can extract the air in the common flow channel 33, the transfer channel, the sample flow channel, and the external sample transfer pipe or the pipe in communication with the transfer pipe, forming a negative pressure, and extracting the sample in the sample collection chamber 411 to the common flow channel 33.

[0179] When it is necessary to extract a reagent, the same operation is performed. The rotor 6 is rotated so that the outer end 62 of the transfer channel is aligned with the circumferential hole 32 that is in communication with the reagent flow channel 37, so that the common flow channel 33 is in communication with the reagent channel. The peristaltic pump 91 discharges the air in the common flow channel 33 and the reagent channel, forming a negative pressure, and extracting the reagent in the reagent chamber 21 to the common flow channel 33.

[0180] When it is necessary to extract the clear water in the clear water collection chamber 412, the same operation is performed, which will not be elaborated here.

[0181] After the sample and reagent are drawn into the common flow channel 33, the rotor 6 continues to rotate so that the outer end 62 of the transfer channel faces the circumferential hole 32 that is in communication with the inner end of the detection flow channel 38. The peristaltic pump 91 drives the sample and reagent in the common flow channel 33 to flow into the detection flow channel 38 from the common flow channel 33, and then flow into the detection chamber from the detection flow channel 38.

[0182] In an embodiment where the mixing chamber 39 is provided, the peristaltic pump 91 can push the sample and the reagent into the mixing chamber 39 from the detection inflow, and push the sample and the reagent from the bottom inlet of the mixing chamber 39 to the top outlet. For the mixing effect, the peristaltic pump 91 can also reverse the flow of the sample and the reagent in the mixing chamber 391 and the mixing bend 392 multiple times. After the sample and the reagent pass through the mixing chamber 391 and multiple mixing bends 392, they are repeatedly mixed to form a mixed liquid. After the mixing effect is achieved, the mixed liquid is finally flowed into the detection chamber from the inflow port 51 of the detection chamber.

[0183] According to the above description, the flow channel plate 3 is further provided with a sewage flow channel 302. The two ends of the sewage flow channel 302 are respectively communicated with the first transfer holes of the other two first transfer columns 3017 at the rear side of the flow channel plate 3. One of the two first transfer columns 3017 is inserted into the flow outlet 52 of the detection chamber, and the first transfer hole of the first transfer column 3017 is communicated with the outflow hole. The other first transfer column 3017 is inserted into the first slot 3016 at the front end of the first transfer member 301 and is communicated with the sewage transfer pipe 3014 located at the top end of the first transfer member 301.

[0184] After the detection is completed, the pump drives air into the detection flow channel 38 and the detection chamber again, and the mixed liquid flows into the sewage flow channel 302 from the flow outlet 52 of the detection chamber, then flows into the sewage transfer pipe 3014 from the sewage flow channel 302, and flows into the sewage channel 416 of the collector from the sewage transfer pipe 3014 and the pipeline, and flows into the toilet due to the sewage channel 416.

[0185] The rotor 6 is rotatably connected to the rear side of the flow channel plate 3. In a specific embodiment, a connecting member 85 is further provided on the rear side surface of the flow channel plate 3. The connecting member 85 is annularly formed by extending outside a plurality of circumferential holes 32 and is located radially outside the rotor 6.

[0186] A protective cover 81 is also covered outside the rotor 6, as Figure 22 and 23 shown. The protective cover 81 covers the connecting member 85 and is also clamped with the connecting member 85, and the rotor 6 rotates in the protective cover 81.

[0187] In addition, as Figure 22 、 Figure 23 shown, in order to drive the rotor 6 to abut against the rear side surface of the flow channel plate 3 and prevent liquid from overflowing from the circumferential holes 32, a compression spring 82 is further provided in the protective cover 81, and a convex ring 83 is provided on the rear side of the rotor 6. The compression spring 82 is sleeved outside the convex ring 83 and the front and rear ends abut against the rear side of the rotor 6 and the rear wall of the protective cover 81, so that the rotor 6 and the flow channel plate 3 are closely matched. Of course, the compression spring 82 can also be replaced with other biasing members, such as magnets attracting each other are respectively provided on the rotor 6 and the flow channel plate 3.

[0188] The driving member 93 is located inside the second housing 12 and is detachably connected to the rotor 6. As Figure 24 shown, the driving member 93 can be a motor. Of course, other driving methods can also be selected. The rotating shaft of the motor extends into the installation groove 1211 of the second housing 12. After the second housing 12 and the first housing 11 are assembled, the rotating shaft of the motor and the rotor 6 can be automatically assembled.

[0189] Specifically, the first rear cover plate 1122 of the first housing 11 is provided with an avoidance hole for avoiding the protection cover 81, and the inner wall of the installation groove 1211 of the second housing 12 is also provided with an avoidance hole for avoiding the rotating shaft of the motor. The rear side of the protection cover 81 is located in the avoidance hole of the first rear cover plate 1122 and is also provided with a through hole, and the rotating shaft of the driving member 93 extends from the avoidance hole on the inner wall of the installation groove 1211 into the protection cover 81. In addition, the rear side of the convex ring 83 is provided with a recessed spline groove 84, as Figure 23 and 24 shown, and the front end of the motor rotating shaft is provided with a spline 931 located in the spline groove 84. When the first housing 11 and the second housing 12 are assembled, the spline 931 of the rotating shaft is inserted into the spline groove 84 to complete the assembly.

[0190] The collector is located outside the first housing 11 and the second housing 12, and the collector is connected to the transfer pipe of the first adapter 301 through a plurality of pipes.

[0191] In Figures 25 - 27 the shown embodiment, the collector includes a collection plate 41, an outer cover plate 43, an inner cover plate 42 and a clean water collection member. The collection plate 41 is disc-shaped and has an outer side and an inner side. The outer side is the side away from the toilet, and the inner side is the side close to the toilet. The outer side of the collection plate 41 is provided with a sample collection cavity 411, and the rear side is provided with a clean water collection cavity 412.

[0192] The sample collection cavity 411 is located at the top of the collection plate 41 and the width gradually increases from the bottom to the top, and opens towards the top end of the collection plate 41. This opening is used to collect samples. The clean water collection cavity 412 is located at the bottom of the collection plate 41 and is arranged in a staggered manner with the sample collection cavity 411.

[0193] The top end of the collection plate 41 is also provided with a water inlet channel 413, a water outlet channel 414, and a sample outlet channel 415. The water inlet channel 413 and the water outlet channel 414 are respectively communicated with the clean water collection cavity 412. The water inlet channel 413 and the water outlet channel 414 are both located at the rear side of the collection plate 41 and are recessed from the top end of the collection plate 41 into the clean water collection cavity 412.

[0194] The sample collection chamber 411 is located on the front side of the collection plate 41 and is a groove formed by a depression on the front side of the collection plate 41. After the collection plate 41 and the outer cover plate 43 are connected and joined together, the sample collection chamber 411 is formed. The top end of the front cover plate is provided with a filtering portion 44, and the filtering portion 44 is a grille arranged at intervals at the top end of the front cover plate. A filter screen or the like can also be added to filter foreign matters.

[0195] The inner walls on both sides of the sample collection chamber 411 are arc surfaces protruding towards each other, so that the width of the sample collection chamber 411 gradually increases from the bottom end to the top end. In addition, the arc-shaped inner wall can increase the opening of the sample collection chamber 411 and also facilitate the sample to flow from the top end to the bottom end.

[0196] The inner wall of the sample collection chamber 411 is also provided with two recessed drainage grooves 4111. The two drainage grooves 4111 are respectively formed by depressions downward from the top end of the collection plate 41 and are arranged at intervals. A partition column 402 is formed between the two drainage grooves 4111. The partition column 402 is basically located in the middle position of the sample collection chamber 411, and the two drainage grooves 4111 are also basically the same in shape. The bottom walls of the two drainage grooves 4111 are also arc surfaces recessed downward.

[0197] The drainage grooves 4111 are convenient for receiving more samples and also for the samples to flow along the inner walls of the drainage grooves 4111, providing a larger flow channel arc surface, facilitating the samples to slowly flow into the bottom end of the sample collection chamber 411 and reducing the generation of bubbles.

[0198] It should be understood that if there is enough space in the sample collection chamber 411, multiple drainage grooves 4111 can also be provided. The multiple drainage grooves 4111 can be arranged at intervals along the width direction of the collector. The inner walls and bottom walls of the multiple drainage grooves 4111 are preferably smooth arc surfaces.

[0199] The bottom end of the sample collection chamber 411 is also provided with a sewage discharge port 417. The sewage discharge port 417 is open towards the bottom end of the collection chamber and the diameter of the sewage discharge port 417 is very small. During the sample collection process, the sewage discharge port 417 is always open. Due to its small diameter, it will not affect the sample collection.

[0200] A liquid level sensor is also provided in the sample collection chamber 411. The liquid level sensor is located below the two drainage grooves 4111 and adjacent to the drainage grooves 4111 for sensing the liquid level of the sample collection.

[0201] In addition, the sample outlet channel 415 is formed by a depression on the rear side of the collection plate 41. The bottom end of the sample outlet channel 415 extends into the sample collection chamber 411 and is located above the sewage discharge port 417, while the top end is located at the upper end of the collection plate 41. The top end of the sample outlet channel 415 is connected to the sample transfer pipe 3011 through a pipe. The sample in the sample collection chamber 411 can flow into the sample flow channel through the sample outlet channel 415 and the sample transfer pipe 3011.

[0202] The clear water collection chamber 412 is formed by a recess at the rear side of the collection plate 41. After the inner cover plate 42 is connected to the rear side of the collection plate 41, the inner cover plate 42 covers the clear water collection chamber 412 to form a closed cavity.

[0203] The clear water collection chamber 412 includes a first chamber 4121 and a second chamber 4122. The first chamber 4121 and the second chamber 4122 are located on both sides of the sample collection chamber 411 and are connected at the bottom. The first chamber 4121 and the second chamber 4122 are symmetrically arranged, and exhaust holes 418 are respectively provided at the top ends.

[0204] The water inlet channel 413 and the water outlet channel 414 are respectively formed by recesses at the rear side of the collection plate 41. The bottom end of the sample outlet channel 415 is connected to the bottom end of the sample collection chamber 411.

[0205] The top end of the water inlet channel 413 is also connected to an external clear water collection member. The clear water collection member can be a pipe connected to a clear water tank or a water switch, or a pipe can be directly provided and adhered to the inner wall of the toilet and above the collector for collecting clear water. The present invention does not limit the specific implementation manner of the clear water collection member.

[0206] The diameter of the water outlet channel 414 is much smaller than the diameter of the water inlet channel 413, and the top end of the water outlet channel 414 can be connected to the clear water adapter pipe 3012 through a pipe.

[0207] That is to say, the water collected by the clear water collection member flows into the clear water collection chamber 412 from the water inlet channel 413. The clear water collection chamber 412 is similar to a water storage tank. The water in the clear water collection chamber 412 then enters the clear water flow channel of the flow channel plate 3 through the water outlet channel 414 and the clear water adapter pipe 3012.

[0208] After the water of the clear water collection member enters the clear water collection chamber 412, bubbles will be generated. The bubbles will float to the water surface. The water outlet channel 414 is located at the bottom end of the clear water collection chamber 412, and the water outlet channel 414 is relatively thin. The bubbles will not enter the water outlet channel 414. After flowing into the clear water adapter pipe 3012 from the water outlet channel 414, there will be no bubbles in the clear water flowing into the clear water flow channel.

[0209] A liquid level sensor is also installed in the clear water collection chamber 412 for sensing the liquid level of the clear water.

[0210] On the rear side of the collection board 41, there is also a sewage discharge channel 416. The sewage discharge channel 416 extends to the top of the collection board 41 and is connected to a sewage discharge adapter 3014 through a pipe. The sewage discharge adapter 3014 is connected to the detection chamber, and the inner cover plate 42 is also provided with a liquid outlet 421 communicating with the sewage discharge channel 416. After the detection is completed, the mixed liquid in the detection chamber can flow into the sewage discharge channel 416 from the sewage discharge channel 302 of the flow channel plate 3 and the sewage discharge adapter 3014 in sequence, and then flow into the toilet from the sewage discharge channel 416 and the liquid outlet 421.

[0211] In Figures 28 - 30 In another embodiment described above, the collector also includes a collection board 41, an outer cover plate 43, an inner cover plate 42 and a clean water collection member. Similarly, the collection board 41 is also disc-shaped and is also provided with a clean water collection chamber 412 and a sample collection chamber 411. The top surface of the collection board 41 is also provided with a water inlet channel 413, a water outlet channel 414 and a sample outlet channel 415.

[0212] The difference is that the sample collection chamber 411 and the clean water collection chamber 412 are formed by the combination of the collection board 41, the inner cover plate 42 and the outer cover plate 43. The sample collection chamber 411 and the clean water collection chamber 412 are through grooves provided on the collection board 41. After the front cover plate and the rear cover plate are covered on the front and rear sides of the collection board 41, the front and rear sides of the sample collection chamber 411 and the clean water collection chamber 412 are closed to form the sample collection chamber 411 and the clean water collection chamber 412.

[0213] The volume of the clean water collection chamber 412 is larger than that of the sample collection chamber 411. The clean water collection chamber 412 and the sample collection chamber 411 are respectively on both sides of the collection board 41, and there is an isolation part in the middle.

[0214] The top surface of the collection board 41 is provided with a sample collection groove 401, and the sample collection groove 401 is formed by the depression of the top surface of the collection board 41.

[0215] The bottom wall of the sample collection groove 401 is also provided with a sampling channel, and the sampling channel is formed by the depression of the bottom wall of the sample collection groove 401 to the sample collection chamber 411.

[0216] The sample outlet channel 415 is also formed by the depression of the bottom wall of the sample collection groove 401 to the bottom of the sample collection chamber 411. The sample outlet channel 415 is located on the isolation part, and the top end of the sample outlet channel 415 is a vertical flow channel extending vertically upward, and the middle area is a bent arc-shaped flow channel, and the bottom end is a horizontally extending channel, and the bottom end extends to the bottom of the sample collection chamber. This bent design of the sample outlet channel 415 can also make the sample fit the inner wall of the sample outlet channel 415 and mix during the flow process to reduce air bubbles.

[0217] The bottom end of the sample collection chamber 411 is also provided with a sewage outlet 417, which is open towards the bottom end of the collection plate 41. Excess samples can flow into the toilet through the sewage flow channel 302.

[0218] The water inlet channel 413 is also formed by the depression of the bottom wall of the sample collection tank 401 to the clean water collection chamber 412, and the water inlet channel 413 is also located on the front side of the collection plate 41. A pipe can be inserted into the top end of the water inlet channel 413, and it is connected to the clean water collection component through this pipe. Similar to the previous embodiment, it will not be elaborated here.

[0219] The water outlet channel 414 is formed by bending upwards from the bottom of the clean water collection chamber 412 to the bottom wall of the sample collection tank 401. The water outlet channel 414 is formed by the depression of the rear side of the collection plate 41 and is also located on the isolation part. The water outlet channel 414 also has a bent shape. During the process of collecting clean water, the air bubbles in the clean water can also be reduced from entering the clean water flow channel of the flow channel plate 3.

[0220] A sewage channel 416 is also provided on the rear side of the isolation part. The top end of the sewage channel 416 extends to the bottom wall of the sample collection tank 401 and is connected to the sewage transfer joint 3014 through a pipe. The bottom end of the sewage channel 416 is connected to the liquid outlet 421 of the inner cover plate 42. The inner cover plate 42 is covered on the rear side of the collection plate 41 and is used to attach to the inner wall of the toilet. The liquid in the detection chamber can flow into the sewage channel 416 from the sewage flow channel 302 and the sewage transfer joint 3014 in sequence, and then flow into the toilet from the liquid outlet 421.

[0221] This collector also includes two liquid level sensors, which are respectively installed in the clean water collection chamber 412 and the sample collection chamber 411 to sense the liquid levels of clean water and samples.

[0222] A sample flow channel can be set on the flow channel plate 3, and this sample flow channel can be directly connected to the sample collection chamber 411 through the sample transfer joint 3011. In another embodiment, two sample flow channels can also be set on the flow channel plate 3. The inner ends of the two sample flow channels are respectively connected to two of the circumferential holes 32, and the outer ends are respectively connected to two pipes through two sample transfer joints 3011.

[0223] Define the two sample flow channels as the upper sample flow channel 341 and the lower sample flow channel 342, and the pipes connected to the two sample flow channels are respectively the upper sample pipe 344 and the lower sample pipe 345. Among them, the upper sample flow channel 341 is connected to the upper sample pipe 344 through the sample transfer joint 3011, and the lower sample flow channel 342 is connected to the lower sample pipe 345 through a sample transfer joint 3011.

[0224] One end of the lower sample pipeline is communicated with the lower sample flow channel 342, and the other end is communicated with the sample outlet channel 415 of the sample collection chamber 411. A burette is provided in the middle of the lower sample pipeline, and this burette is defined as the sample burette 343. One end of the upper sample pipeline is communicated with the upper sample flow channel 341, and the other end is communicated above the sample burette 343. When it is necessary to extract a sample, first align the rotor 6 with the circumferential hole 32 communicated with the upper sample flow channel 341, evacuate the air in the upper sample flow channel 341, the upper sample pipeline and the sample burette 343, and create a negative pressure in the sample burette 343, so that the sample can drip into the sample burette 343 from the sample collection chamber 411. Then rotate the rotor 6 again to align the transfer channel with the circumferential hole 32 of the lower sample flow channel 342, evacuate the air in the lower sample flow channel 342 and the lower sample pipeline, and let the liquid in the sample burette 343 flow into the lower sample flow channel 342 through the lower sample pipeline. This setting can ensure that the sample drips slowly into the lower sample flow channel 342 without generating bubbles.

[0225] Of course, in another embodiment, an exhaust hole 418 is provided in the sample collection chamber 411. The upper sample flow channel 341 can be communicated with the exhaust hole 418, and the lower sample flow channel 342 is communicated with the sample outlet channel 415 of the sample collection chamber 411. At this time, the sample collection chamber 411 is similar to the above-mentioned sample burette, and the operation method is the same as above, so it will not be elaborated here.

[0226] One clear water flow channel can also be provided on the flow channel plate 3, and this clear water flow channel is directly communicated with the clear water collection chamber 412.

[0227] Of course, two clear water flow channels are provided on the flow channel plate 3. Based on the same principle as the above two sample flow channels, the two clear water flow channels are respectively the upper clear water flow channel 351 and the lower clear water flow channel 352. The outer ends of the two clear water flow channels are respectively communicated with the two circumferential holes 32. Similarly, the outer end of the lower clear water flow channel is communicated with the clear water adapter tube 3012, and the clear water adapter tube 3012 is communicated with the clear water collection chamber 412 through the upper clear water pipeline 354. A clear water burette 353 is also provided in the lower clear water pipeline 355. The clear water burettes 353 of the upper clear water pipeline 354 and the lower clear water pipeline 355 are communicated.

[0228] The upper clear water flow channel 351 is communicated with the clear water adapter tube 3012, and this clear water adapter tube 3012 is communicated with the upper end of the clear water burette 353 through the upper clear water pipeline 354.

[0229] When a sample needs to be extracted, the rotor 6 needs to be aligned with the circumferential hole 32 communicating with the upper clear water flow channel 351 first. The air in the upper clear water flow channel 351, the upper sample clear water pipeline, and the clear water burette 353 is extracted, and a negative pressure is formed in the clear water burette, so that the clear water can drip into the clear water burette 353 from the clear water collection cavity 412. Then the rotor 6 is rotated to align the transfer channel with the circumferential hole 32 of the lower clear water flow channel 352, and the air in the lower clear water flow channel 352 and the lower clear water pipeline is extracted, and the liquid in the clear water burette 353 flows into the lower clear water flow channel 352 from the lower clear water pipeline. This setting can ensure that the water drips slowly into the lower clear water flow channel 352 without generating bubbles.

[0230] The present invention also relates to a control method for a urine analyzer 100. The urine analyzer 100 includes a reagent kit 2, a sampler, a rotary valve, and the above-mentioned detection mechanism. The reagent kit 2 is the same as above, and is provided with a plurality of reagent chambers 21, and reagents are placed in the reagent chambers 21. The sampler is also provided with the above-mentioned sample collection cavity 411 and clear water collection cavity 412. The rotary valve is the same as above and includes a microfluidic chip and a rotor 6. The microfluidic chip is also provided with a common flow channel 33, a lower sample flow channel 342, an upper sample flow channel 341, an upper clear water flow channel 351, a lower clear water flow channel 352, a plurality of reagent flow channels 37, a detection flow channel 38, an air flow channel 36, and a transfer channel. The outer end of the lower sample flow channel 342 communicates with the sample collection cavity 411 through a lower sample pipeline 345, and the lower sample pipeline 345 is provided with a sample burette 343. The outer end of the upper sample flow channel 341 communicates with the top of the sample burette 343. The outer ends of the plurality of reagent flow channels 37 respectively communicate with the plurality of reagent chambers 21. The outer end of the lower clear water flow channel 352 communicates with the clear water collection cavity 412. The rotor 6 is provided with a transfer channel. When the rotor 6 rotates, the transfer channel can be driven to rotate. The inner end 61 of the transfer channel is located at the center of the rotor 6 and communicates with the inner end of the common flow channel 33, and the outer end rotates with the rotor 6 and communicates with the inner ends of the lower sample flow channel 342, the upper sample flow channel, the upper clear water flow channel 351, the lower clear water flow channel 352, the plurality of reagent flow channels 37, the detection flow channel 38, and the air flow channel 36. That is to say, every time the rotary valve switches to the lower sample flow channel 342, the upper sample flow channel, the upper clear water flow channel 351, the lower clear water flow channel 352, the plurality of reagent flow channels 37, the detection flow channel 38, and the air flow channel 36, the transfer channel communicates with the lower sample flow channel 342, the upper sample flow channel, the upper clear water flow channel 351, the lower clear water flow channel 352, the plurality of reagent flow channels 37, the detection flow channel 38, and the air flow channel 36.

[0231] The detection mechanism is the same as above, and is provided with a detector and a detection cavity. The detection cavity communicates with the detection flow channel 38, and the detector is used to detect the sample in the detection cavity.

[0232] As Figure 31 shown, the control method of the urine analyzer 100 includes the steps:

[0233] S1. The rotary valve switches to the upper sample flow channel 341. The transfer channel communicates with the upper sample flow channel 341, and the air in the sample burette 343 is pumped into the transfer channel and the common flow channel 33. A negative pressure is formed in the sample burette 343, and the sample in the sample collection chamber 411 enters the sample burette 343. When the rotary valve switches to the upper sample flow channel and the common flow channel communicates with the upper sample flow channel, the air in the sample burette 343 can be pumped into the common flow channel 33, causing a negative pressure to be formed in the sample burette 343. After the sample enters the sample burette 343, it flows to the bottom of the sample burette 343 under the action of gravity.

[0234] S2. The rotary valve switches to the lower sample flow channel 342. The transfer channel communicates with the lower sample flow channel 342, and the sample in the sample burette 343 is pumped into the common flow channel 33 or the transfer channel. At this time, a part of the sample enters the transfer channel, and most of it stays in the lower sample flow channel 342, facilitating subsequent quantitative extraction.

[0235] S3. The rotary valve switches to the lower clear water flow channel 352. The transfer channel communicates with the lower clear water flow channel 352, and the water in the clear water collection chamber 412 is pumped into the common flow channel 33 or the transfer channel. Because the transfer channel and the common flow channel 33 are connected, when the amount of sample and water entering the transfer channel is large, it may enter the common flow channel 33. The water and the sample entering the common flow channel 33 or the transfer channel are mixed.

[0236] S4. The sample and water in the common flow channel 33 are discharged from the outer end of the common flow channel 33. It can flow out from the outer end of the common flow channel 33 or from the detection chamber, and the specific discharge method is not limited.

[0237] S5. The rotary valve switches to the lower sample flow channel 342. The transfer channel communicates with the lower sample flow channel 342, and a quantitative amount of the sample in the lower sample flow channel 342 is pumped into the common flow channel 33 or the transfer channel. In step S2, the sample is pumped into the lower sample flow channel 342, and the sample in the lower sample flow channel 342 basically flows to the inner end of the lower sample flow channel 342. Therefore, the sample can be quantitatively extracted according to the duration of sample extraction.

[0238] S6. The rotary valve switches to the reagent flow channel 37. The transfer channel communicates with the reagent flow channel 37, and a quantitative amount of the reagent in the reagent chamber 21 is pumped into the common flow channel 33 or the transfer channel. The reagent flow channel 37 has been in communication with the reagent chamber 21, so a part of the reagent in the reagent chamber 21 is located in the reagent flow channel 37 and can be directly quantitatively extracted.

[0239] S7. The rotary cutting valve switches to the detection flow path 38. The transfer channel communicates with the detection flow path 38, and the sample and reagent in the common flow path 33 or the transfer channel are pushed into the detection chamber.

[0240] S8. The detector detects the sample in the detection chamber.

[0241] The outer end of the transfer channel of the rotor communicates with different flow paths as the rotary cutting valve switches. For example, when the rotary cutting valve switches to the reagent flow path, the outer end of the transfer channel communicates with this reagent flow path. The same applies when the rotary cutting valve switches to other flow paths, which will not be elaborated here.

[0242] Further, the urine analyzer 100 includes a first pump and a second pump. The first pump is the peristaltic pump 91 mentioned above, and the second pump is the plunger pump 92. The first pump is connected to a first drive pipeline, and the first drive pipeline communicates with the outer end of the common flow path 33. The second pump is connected to a second drive pipeline, and the second drive pipeline communicates with the outer end of the common flow path 33.

[0243] In the above steps, in step S1, the first pump rotates forward to extract the air in the sample burette 343.

[0244] In step S2, the first pump rotates forward to suck the sample in the sample burette 343 into the common flow path 33.

[0245] In step S3, the first pump rotates forward to suck the water in the clean water collection chamber 412 into the common flow path 33.

[0246] In step S4, the first pump rotates forward to discharge the sample and water in the common flow path 33 through the outer end of the first drive pipeline.

[0247] In step S5, the second pump rotates forward and quantitatively extracts the sample in the lower sample flow path 342 into the common flow path 33.

[0248] In step S6, the second pump rotates forward and quantitatively extracts the reagent in the reagent chamber 21 into the common flow path 33.

[0249] In step S7, the second pump rotates in reverse to push the sample and reagent in the common flow path 33 into the detection chamber.

[0250] That is to say, the stroke of the first pump is large, and it can extract a large amount of clean water or samples, while the second pump is mainly responsible for quantitatively extracting samples and reagents.

[0251] Optionally, the lower clean water flow path 352 communicates with the clean water collection chamber 412 through a lower clean water pipeline 355, and the lower clean water pipeline 355 is provided with a clean water burette 353;

[0252] The rotary cutting valve is provided with an upper clear water flow path, and the upper clear water flow path 351 communicates with the clear water burette 353;

[0253] In step S3, the rotary cutting valve switches to the upper clear water flow path 351, the transfer channel communicates with the upper clear water flow path 351, the first pump rotates forward, and the air in the clear water burette 353 is pumped out. A negative pressure is formed in the clear water burette 353. After the clear water in the clear water collection chamber 412 enters the clear water burette 353, the rotary cutting valve switches to the lower clear water flow path 352, and the first pump rotates forward to pump the water in the clear water burette 353 into the lower clear water flow path 352.

[0254] Optionally, before step S6, it is judged whether multiple reagents need to be extracted. If one reagent is needed, step S6 is carried out; if multiple reagents are needed, step S6 is repeated, then the rotary cutting valve is switched to the detection flow path, the reagent and the sample in the transfer channel are pushed into the detection flow path, and then the rotary cutting valve is switched to different reagent flow paths, different reagents are extracted into the transfer channel, and then the extracted reagents are repeatedly pushed into the detection flow path.

[0255] That is to say, each time the rotary cutting valve is switched to a different reagent flow path, a reagent is extracted into the transfer channel, then the rotary cutting valve is switched to the detection flow path, the reagent in the transfer channel is pushed into the detection flow path to react with the sample, and then after waiting for a few seconds or a preset time, the rotary cutting valve is switched to a different reagent flow path again, different reagents are extracted into the detection flow path and then react with the sample. The reaction time or the waiting time can be set according to the basic requirements. In some embodiments, the waiting time may not be set either.

[0256] Some detection items can be completed with one reagent, and some items require two or more reagents. When carrying out detection items that require multiple reagents, step S6 needs to be repeated multiple times, and different reagents are extracted each time.

[0257] It should be understood that whether the detection item needs dilution, the amount of diluted sample required for detection, the type of reagent required, and the amount of reagent are set in the control module. Therefore, after the detection is started, the control module will judge the process to be carried out according to the detection item.

[0258] Optionally, in step S5, after the second pump sucks the sample in the lower sample flow path 342 into the common flow path 33, the following steps are further included: the rotary cutting valve is switched to the lower clear water flow path 352, and the second pump quantitatively extracts the water in the lower clear water flow path 352 into the common flow path 33 and mixes it with the sample in the common flow path 33 to dilute the sample. Some detection items require mixing the sample with clear water to dilute the sample. Therefore, after extracting the sample, a quantitative amount of clear water also needs to be extracted.

[0259] In step S6, after the second pump rotates forward to push the reagent in the reagent chamber 21 into the common flow channel 33, the following steps are further included: the rotary cutting valve switches to the air flow channel 36, and the second pump rotates forward to absorb air into the transfer channel.

[0260] Optionally, in step S5, after the second pump quantitatively extracts the water in the lower clear water flow channel 352 into the common flow channel 33 and mixes it with the sample in the common flow channel 33, the sample is diluted for 8s - 12s. The dilution time is preferably 10s.

[0261] As a preferred solution, the rotary cutting valve is further provided with a mixing chamber 39, and the mixing chamber 39 is located on the path of the detection flow channel 38 and communicates with the detection flow channel 38;

[0262] In step S6, after the second pump quantitatively extracts the reagent in the reagent chamber 21 into the common flow channel 33, the following steps are further included: the rotary cutting valve switches to the air flow channel 36, and the second pump rotates forward to quantitatively extract air into the common flow channel 33. Similarly, due to the stroke problem, a part of air needs to be extracted.

[0263] In step S7, the rotary cutting valve switches to the detection flow channel 38, and the second pump rotates in reverse to push the sample and reagent in the common flow channel 33 into the mixing chamber 39;

[0264] The first pump rotates forward for a preset time and then rotates in reverse for a preset time to make the reagent and sample flow continuously in the mixing chamber 39 and form a mixed liquid. The peristaltic pump 91 makes the reagent and sample flow continuously in the mixing chamber 39 and mixes them.

[0265] Then the second pump rotates forward to push the mixed liquid in the mixing chamber 39 into the detection chamber.

[0266] Further, the mixing chamber 39 includes a mixing bend 392 and a mixing chamber 391 that communicate with each other, and the mixing bend 392 is a curved flow channel.

[0267] In step S7, after the second pump rotates in reverse to push the sample and reagent in the common flow channel 33 into the mixing chamber 39, the first pump rotates forward for a preset time and then rotates in reverse for a preset time to make the reagent and sample flow continuously in the mixing bend 392 and the mixing chamber 39 and form a mixed liquid.

[0268] Optionally, the rotary cutting valve is in a vertical state and is provided with a mixing chamber 39. The mixing chamber 39 is located on the path of the detection flow channel 38 and communicates with the detection flow channel 38. The mixing chamber 39 has an inlet and an outlet, and the inlet is located below the outlet. The flow channel plate 3 is placed in a vertical state. The mixing chamber 39 is located above the common flow channel 33. The inlet of the mixing chamber 39 is at the lower part, and the outlet is at the upper part. The outlet refers to the end opening that flows into the detection chamber.

[0269] In step S6, after the second pump quantitatively extracts the reagent in the reagent chamber 21 into the common flow channel 33, the following steps are further included: the rotary cutting valve switches to the air flow channel 36, and the second pump rotates forward to quantitatively extract air into the common flow channel 33;

[0270] In step S7, the rotary cutting valve switches to the detection flow channel 38, and the second pump rotates in reverse to push the sample and reagent in the common flow channel 33 from the inlet to the top of the mixing chamber 39. The sample and reagent move downward by gravity. Then, after the first pump rotates in reverse for a preset time, the reagent and sample are pushed into the top of the mixing chamber 39 again. Then the first pump stops moving, and the sample and reagent move downward by gravity again to the bottom of the mixing chamber 39 and flow continuously to form a mixed liquid;

[0271] Finally, the second pump rotates forward to push the mixed liquid in the mixing chamber 39 into the detection chamber.

[0272] Preferably, after step S8, the following step S9 is further included: the rotary cutting valve switches to the lower clear water flow channel 352, and the first pump rotates forward to extract the water in the lower clear water flow channel 352 into the common flow channel 33. Then the rotary cutting valve switches to the detection flow channel 38, and the first pump rotates in reverse to push the water in the common flow channel 33 into the detection flow channel 38 and the detection chamber and then discharge it. After the detection is completed, the detection chamber is cleaned with clear water again.

[0273] Optionally, after step S8, the following step S9 is further included: the rotary cutting valve switches to the lower clear water flow channel 352, and the first pump rotates forward to extract the water in the lower clear water flow channel 352 into the common flow channel 33. Then the rotary cutting valve switches to the air flow channel 36, and the first pump rotates forward to extract air and then discharge the water in the common flow channel 33 from the first drive pipeline. After the detection is completed, the common flow channel 33 and the first drive pipeline are cleaned with clear water. The first drive pipeline is connected to the collector, and the waste liquid can be discharged from the collector into the toilet.

[0274] Optionally, after step S8, step S9 is further included: the rotary cutting valve is switched to communicate with the lower clear water flow channel 352, the first pump rotates forward to extract the water in the lower clear water flow channel 352 into the common flow channel 33, then the rotary cutting valve is switched to communicate with the lower sample flow channel 342, the first pump rotates in reverse and pushes the water in the common flow channel 33 into the lower sample pipe 345, and discharges it from the sample collection chamber 411. After the detection is completed, clear water is extracted again to clean the lower sample flow channel 342, the lower sample pipe 345 and the sample collection chamber 411, and the waste liquid is discharged from the sample collection chamber 411.

[0275] After the urine analyzer 100 has been used for several months or operated a preset number of times, the reagents in the reagent kit 2 are used up and the reagent kit 2 needs to be replaced. When replacing the reagent kit 2, the reagent flow channel 37 needs to be thoroughly cleaned, and the washing flow channel of the flow channel plate 3 needs to be used.

[0276] The urine analyzer 100 further includes a washing liquid and a washing chamber for containing the washing liquid.

[0277] Replacing the reagent kit includes the steps of:

[0278] S01: The rotary cutting valve is switched to the lower clear water flow channel 352, the first pump rotates forward and quantitatively extracts clear water into the common flow channel 33, then the rotary cutting valve is sequentially switched to a plurality of the reagent flow channels 37, the first pump rotates in reverse in sequence, and pushes the water in the common flow channel 33 into the plurality of reagent flow channels 37 in sequence, and flows into the plurality of reagent chambers 21 from the plurality of reagent flow channels 37.

[0279] S02: Replace the reagent kit 2.

[0280] After cleaning the reagent flow channel 37 with clear water, the waste liquid is pushed into the reagent chamber 21 of the reagent kit 2, and then the reagent kit 2 is replaced.

[0281] Optionally, before step S01, the following steps are further included:

[0282] The rotary cutting valve is switched to the lower clear water flow channel 352, the first pump rotates forward to extract clear water into the common flow channel 33, then the rotary cutting valve is switched to the air flow channel 36, the first pump rotates forward to extract air into the common flow channel 33, and discharges the clear water in the common flow channel 33 from the first driving pipe;

[0283] The rotary cutting valve is switched to the lower clear water flow channel 352 again, the first pump rotates forward to extract clear water into the common flow channel 33, then the rotary cutting valve is switched to the lower sample flow channel 342, and the first pump rotates in reverse to push the clear water in the common flow channel 33 into the lower sample pipe 345 and discharges it from the sample collection chamber 411;

[0284] The rotary valve switches to the lower clear water flow path 352 again, and the first pump rotates forward to draw clear water into the common flow path 33; then the rotary valve switches to the detection flow path 38, and the first pump rotates in reverse to push the clear water in the common flow path 33 into the detection flow path 38 and discharge it from the detection chamber.

[0285] First, draw clear water to wash the common flow path 33, the sample collection chamber 411 and the detection chamber, and then clean the reagent flow path 37.

[0286] Optionally, before step S01, the following steps are further included:

[0287] The rotary valve switches to the lower clear water flow path 352, the first pump rotates forward and quantitatively draws clear water into the common flow path 33, and then switches to the washing flow path, and the second pump rotates forward and sucks the washing liquid in the washing chamber into the common flow path 33;

[0288] Then the rotary valve switches to the detection flow path 38, the first pump rotates in reverse and sucks the clear water and washing liquid in the common flow path 33 into the mixing chamber 39, then the first pump rotates forward for a preset time and then rotates in reverse for a preset time to make the clear water and washing liquid in the mixing chamber 39 flow continuously and be mixed into a diluted washing liquid, and then the first pump rotates forward and sucks the diluted washing liquid in the mixing chamber 39 into the common flow path 33;

[0289] The rotary valve sequentially switches to multiple reagent flow paths 37, and the first pump rotates in reverse and sequentially pushes the diluted washing liquid in the common flow path 33 into multiple reagent chambers 21.

[0290] After cleaning the common flow path 33, the sample collection chamber 411 and the detection chamber with clear water, then repeat mixing the washing liquid and clear water to form a diluted washing liquid, after cleaning the reagent flow path 37 with the diluted washing liquid, then perform step S01.

[0291] After the urine analyzer 100 operates for a preset period, for example, after several months, it is also necessary to thoroughly clean the internal structure, which includes the following steps:

[0292] S001: The rotary valve is connected to the lower clear water flow path 352, the first pump rotates forward and quantitatively draws clear water into the common flow path 33, and then the rotary valve is connected to the washing flow path, and the second pump rotates forward and sucks the washing liquid in the washing chamber into the common flow path 33;

[0293] S002: Then the rotary valve switches to the detection flow channel 38, the first pump reverses and sucks the clear water and the washing liquid in the common flow channel 33 into the mixing chamber 39. Then, after the first pump rotates forward for a preset time and then reverses for a preset time, the clear water and the washing liquid in the mixing chamber 39 flow continuously and are mixed into a diluted washing liquid. Then the first pump rotates forward and sucks the diluted washing liquid in the mixing chamber 39 into the common flow channel 33. Diluted washing liquid is required for thorough cleaning, so water and washing liquid are mixed first.

[0294] S003: The rotary valve switches to the lower sample flow channel 342, and the first pump reverses to push the diluted washing liquid in the common flow channel 33 from the lower sample pipeline 345 into the sample collection chamber 411.

[0295] S004: Repeat S001 and S002, and then the first pump reverses to push the diluted washing liquid in the common flow channel 33 into the detection chamber.

[0296] S005: After waiting for a preset time, the rotary valve switches to the lower clear water flow channel 352. After the first pump rotates forward and quantitatively extracts clear water into the common flow channel 33, the clear water in the common flow channel 33 is pushed into the detection chamber, the first drive pipeline, and the clear water collection chamber and discharged.

[0297] After the diluted washing liquid is soaked in the sample collection chamber 411 and the detection chamber for a preset time, it is then discharged.

[0298] Optionally, step S005 includes:

[0299] S0051: The rotary valve switches to the lower clear water flow channel 352. After the first pump rotates forward and quantitatively extracts clear water into the common flow channel 33, the rotary valve switches to the detection flow channel 38, and the first pump reverses to push the clear water in the common flow channel 33 into the detection chamber and discharge it.

[0300] S0052: The rotary valve switches to the lower clear water flow channel 352. After the first pump rotates forward and quantitatively extracts clear water into the common flow channel 33, the rotary valve switches to the air flow channel 36, and the first pump rotates forward to extract external air into the common flow channel 33. Then the clear water in the common flow channel 33 is discharged from the first drive pipeline.

[0301] S0053: The rotary valve switches to the lower clear water flow channel 352. After the first pump rotates forward and quantitatively extracts clear water into the common flow channel 33, the rotary valve switches to the lower sample flow channel 342, and the first pump reverses to push the clear water in the common flow channel 33 into the sample collection chamber 411 and discharge it.

[0302] First, pump water into the common flow channel 33, and then use water to push the diluted washing liquid out of the sample collection chamber 411, the detection chamber, and the first drive pipe.

[0303] Optionally, before step S001, the following steps are further included:

[0304] The rotary cutting valve is switched to the lower clear water flow channel 352, the first pump rotates forward to pump clear water into the common flow channel 33, then the rotary cutting valve is switched to the air flow channel 36, the first pump rotates forward to pump air into the common flow channel 33, and discharges the clear water in the common flow channel 33 from the first drive pipe;

[0305] The rotary cutting valve is switched to the lower clear water flow channel 352, the first pump rotates forward to pump clear water into the common flow channel 33, then the rotary cutting valve is switched to the lower sample flow channel 342, and the first pump rotates in reverse to push the clear water in the common flow channel 33 into the lower sample pipe 345 and discharge it from the sample collection chamber 411;

[0306] The rotary cutting valve is switched to the lower clear water flow channel 352, the first pump rotates forward to pump clear water into the common flow channel 33, then the rotary cutting valve is switched to the detection flow channel 38, and the first pump rotates in reverse to push the clear water in the common flow channel 33 into the detection flow channel 38 and discharge it from the detection chamber.

[0307] The urine analyzer further includes a control module. After step S8, that is, after the detection is completed, the detector sends the detection result to the control module. If the detection result is abnormal, the control module restarts steps S1 to S8; if the detection result is normal, the next detection item is carried out.

[0308] Further, the urine analyzer further includes a warning light, and the warning light is connected to the control module;

[0309] After step S8, if the detection results are abnormal for two consecutive times, the control module controls the warning light to give a warning.

[0310] In addition, the control module is also connected to the liquid level sensors in the clear water collection chamber and the sample collection chamber.

[0311] Before step S1, the liquid level sensor in the sample collection chamber can send the liquid level signal in the sample collection chamber to the control module. If the liquid level meets the requirements, then S1 is carried out; if it does not meet the requirements, the process ends.

[0312] In the same way as in step S3, the control module receives the liquid level of the liquid level sensor in the clear water collection chamber to judge whether the clear water level meets the requirements. If it meets the requirements, the next step is carried out. If it does not meet the requirements, the process ends.

[0313] In addition, the urine analyzer further includes a fingerprint module or an induction module, which is connected to the control module. The operator can turn on the urine analyzer through fingerprint control, or after the induction module senses the operator's action, it transmits a signal to the control module to start the operation.

[0314] The present invention also relates to a method for using a microfluidic chip. The flow channel plate 3 is provided with the above-mentioned sample flow channel, reagent flow channel 37, detection flow channel 38, and common flow channel 33. The common flow channel 33 is operably connected to the sample flow channel, reagent flow channel 37, and detection flow channel 38. The mixing chamber 39 is located on the path of the detection flow channel 38 and has an outlet and an inlet. The mixing chamber 39 includes a mixing bend 392 and a mixing chamber 391. The mixing bend 392 is a curved flow channel and is located on the path of the detection flow channel 38. The width dimension of the mixing chamber 391 is larger than the diameter dimension of the detection flow channel 38.

[0315] The method for using the microfluidic chip includes the steps:

[0316] S11. Driving the sample in the sample flow channel into the common flow channel 33;

[0317] S12. Driving the reagent in the reagent flow channel 37 into the common flow channel 33;

[0318] S13. Driving the sample and the reagent in the common flow channel 33 to enter the inlet of the mixing chamber 39 from the detection flow channel 38, flowing into the mixing chamber 391 after entering the mixing bend 392 from the inlet of the mixing chamber 39 to form a mixed liquid, and then driving the mixed liquid to flow back into the detection flow channel 38 from the outlet of the mixing chamber 39 and flowing out from the detection flow channel 38.

[0319] The above-mentioned sample flow channel is connected to the sample collection chamber, the reagent flow channel 37 is connected to the reagent chamber 21 for storing the reagent, and the detection flow channel 38 is connected to the detection chamber.

[0320] The common flow channel 33 can be connected to the sample flow channel, reagent flow channel 37, or detection flow channel 38 through a three-way valve or other valves. The air in the common flow channel 33 can be pumped out to form a negative pressure by using a peristaltic pump 91 or a plunger pump 92, and then the sample in the above-mentioned sample flow channel or the reagent in the reagent flow channel 37 is driven into the common flow channel 33.

[0321] Optionally, the mixing chamber 391 is located above the mixing bend 392, and the inlet of the mixing chamber 39 is below the outlet of the mixing chamber 39;

[0322] In step S13, the sample and reagent in the common flow channel 33 are driven to enter the inlet of the mixing chamber 39 from the detection flow channel 38, enter the mixing bend 392 from the inlet of the mixing chamber 39 and then flow into the mixing chamber 391. Then, the mixed liquid flows back from the mixing chamber 39 into the mixing bend 392, and the sample and reagent flow back by gravity. Then, drive the mixed liquid to flow from the mixing bend 392 into the mixing chamber 39, and finally drive the mixed liquid to enter the detection flow channel 38 again from the mixing chamber 39 and flow out from the detection flow channel 38.

[0323] Optionally, the mixing chamber 391 is located above the mixing bend 392; in step S3, the sample and reagent in the common flow channel 33 are driven to enter the inlet of the mixing chamber 39 from the detection flow channel 38, enter the mixing bend 392 from the inlet of the mixing chamber 39 and then flow into the mixing chamber 391. Then, the mixed liquid flows back from the mixing chamber 391 into the mixing bend 392, stays for 1 s - 2 s, and then drives the mixed liquid to flow from the mixing bend 392 into the mixing chamber 391. Finally, drive the mixed liquid to enter the detection flow channel 38 again from the mixing chamber 391 and flow out from the detection flow channel 38.

[0324] During the mixing process of the sample and reagent, staying for 1 s - 2 s can improve the mixing effect.

[0325] As a preferred solution, the mixing chamber 39 includes a plurality of mixing bends 392 and a plurality of mixing chambers 391, and the plurality of mixing bends 392 and the plurality of mixing chambers 391 are arranged at intervals and communicate with each other;

[0326] In step S13, the sample and reagent in the common flow channel 33 are driven to enter the inlet of the mixing chamber 39 from the detection flow channel 38, enter a plurality of mixing bends 392 and a plurality of mixing chambers 39 from the inlet of the mixing chamber 39, and finally drive the mixed liquid to flow out from the detection flow channel 38.

[0327] Furthermore, the mixing bend 392 is an S-shaped bend and includes at least two horizontal flow channels and at least one turning flow channel 394, and any one turning flow channel 394 is located at both ends of the two horizontal flow channels;

[0328] The horizontal flow channel extends in the horizontal direction, and the turning flow channel 394 includes a curved inner arc 395 and an outer arc 396. Both the inner arc 395 and the outer arc 396 are arc-shaped and the inner diameter of the inner arc 395 is smaller than the inner diameter of the outer arc 396.

[0329] Optionally, the inner wall of the mixing chamber 391 includes a first arc surface 397 and a second arc surface 398 opposite to the first arc surface 397, and both the first arc surface 397 and the second arc surface 398 are arc surfaces protruding towards the outside.

[0330] Optionally, each mixing chamber 391 communicates with the horizontal flow channels of two mixing bends 392, and the two horizontal flow channels are radially symmetrically arranged at the top and bottom of the mixing chamber 391;

[0331] In step S13, the sample and reagent in the common flow channel 33 are driven to enter the mixing cavity 39 from the detection flow channel 38. After entering from the inlet of the mixing cavity 39, they flow into the mixing chamber 391 after passing through a mixing bend 392, and then flow into another mixing bend 392 from the mixing cavity 39 to form a mixed liquid. After staying for 1 s - 2 s, the mixed liquid flows back into the mixing chamber 391, and the mixed liquid is driven to be discharged from the mixing bend 392.

[0332] In one embodiment, each mixing chamber has a sample inlet and a sample outlet respectively. The sample inlet and the sample outlet of each mixing cavity are respectively communicated with the adjacent mixing bends, and the sample inlet of each mixing cavity is located above the sample outlet.

[0333] In step S13, after the sample and reagent enter the mixing cavity, they flow through one of the mixing bends, enter the adjacent sample inlet, then enter the mixing cavity, and then flow into another mixing bend from the sample outlet. The sample and reagent flow continuously in multiple mixing chambers and multiple mixing bends to form the mixed liquid.

[0334] The microfluidic chip can be placed vertically. That is to say, when the sample and reagent flow from bottom to top, the influence of gravity does not need to be considered, so that there will be no residue problem in the mixing chamber.

[0335] Optionally, the flow channel plate 3 is further provided with a clean water flow channel, and the common flow channel 33 can be communicated with the clean water flow channel through the transfer channel of the rotor 6.

[0336] After step S13, it further includes step S14: driving the water in the clean water flow channel to enter the common flow channel 33, then driving the water in the common flow channel 33 to enter the detection flow channel 38, then entering the mixing cavity 39 from the inlet of the mixing cavity 39, and then entering the detection flow channel 38 again from the outlet of the mixing cavity 39 and being discharged from the detection flow channel 38.

[0337] As a preferred solution, the flow channel plate 3 is further provided with a central hole 31 and a plurality of circumferential holes 32 arranged around the central hole 31. The central hole 31 penetrates the flow channel plate 3 and is communicated with the inner end of the common flow channel 33. The plurality of circumferential holes 32 are arranged circumferentially with the central hole 31 as the center. The inner end of the common flow channel 33 is communicated with the central hole 31, and the outer end is connected to a pump through a driving pipeline. The inner end of the sample flow channel is communicated with one of the circumferential holes 32. The inner end of the reagent flow channel 37 is communicated with one of the circumferential holes 32. The inner end of the detection flow channel 38 is communicated with one of the circumferential holes 32.

[0338] The central hole 31 is communicated with the inner end of the transfer channel of a rotor 6, and the outer end of the transfer channel can be communicated with any one of the circumferential holes 32 as the rotor 6 rotates.

[0339] In step S11, the rotor 6 rotates, and the transfer channel communicates with the sample channel through one of the circumferential holes 32. The pump evacuates the air in the common channel 33 and the sample channel, and pumps the sample in the sample channel into the common channel 33;

[0340] In step S12, the rotor 6 rotates, and the transfer channel communicates with the reagent channel 37 through one of the circumferential holes 32. The pump evacuates the air in the common channel 33 and the reagent channel 37, and pumps the sample in the reagent channel 37 into the common channel 33;

[0341] In step S13, the rotor 6 rotates, and the transfer channel communicates with the detection channel 38 through one of the circumferential holes 32; the pump drives the sample and reagent in the common channel 33 to enter the inlet of the mixing chamber 39 from the detection channel 38, flows into the mixing chamber 391 after entering the mixing bend 392 from the inlet of the mixing chamber 39 to form a mixed liquid, and then drives the mixed liquid to flow into the detection channel 38 again from the outlet of the mixing chamber 39 and flows out from the detection channel 38.

[0342] As another preferred solution, the flow channel plate 3 is provided with two sample channels, namely an upper sample channel 341 and a lower sample channel 342. The inner ends of the lower sample channel 342 and the upper sample channel 341 are respectively communicated with two circumferential holes 32. The lower sample channel is communicated with the sample collection chamber containing the sample through a lower sample pipe, and the above-mentioned sample burette is provided on the lower sample pipe. The upper sample channel 341 is communicated with the sample burette through an upper sample pipe;

[0343] In step S11, the rotor 6 rotates, aligns the outer end 62 of the transfer channel with the circumferential hole 32 communicated with the upper sample channel 341, and the pump cooperates with the driving pipe to evacuate the air in the common channel 33, the transfer channel, the upper sample channel 341 and the sample burette.

[0344] Then the rotor 6 rotates, aligns the outer end 62 of the transfer channel with the circumferential hole 32 communicated with the lower sample channel 342, and pumps the sample in the sample collection chamber from the lower sample channel 342 to the common channel 33.

[0345] Setting the sample burette can reduce the bubbles in the sample collection chamber from flowing into the sample channel.

[0346] Optionally, the flow channel plate 3 is further provided with a clean water channel, and the clean water channel is communicated with one of the circumferential holes 32.

[0347] After step S13, step S14 is further included: the rotor 6 rotates, the transfer channel communicates with the clean water channel through one of the circumferential holes 32, the pump drives the water in the clean water channel into the common channel 33, then drives the water in the common channel 33 into the detection channel 38, then enters the mixing chamber 39 from the inlet of the mixing chamber 39, and then enters the detection channel 38 again from the outlet of the mixing chamber 39 and is discharged from the detection channel 38.

[0348] Preferably, the flow channel plate 3 is provided with an upper clean water channel 351 and a lower clean water channel 352. The inner ends of the upper clean water channel 351 and the lower clean water channel 352 are respectively communicated with two circumferential holes 32. The lower clean water channel is communicated with the clean water collection chamber for storing water through a lower clean water pipe, and a clean water burette is arranged on the lower clean water pipe. The upper clean water channel 351 is communicated with the clean water burette through an upper clean water pipe.

[0349] After step S13, step S14 is further included: the rotor 6 rotates, aligns the outer end 62 of the transfer channel with the circumferential hole 32 communicated with the upper clean water channel 351, and the pump pumps the air in the common channel 33, the transfer channel, the upper clean water channel 351 and the clean water burette to the outside.

[0350] Then the rotor 6 rotates, aligns the outer end 62 of the transfer channel with the circumferential hole 32 communicated with the lower clean water channel 352, pumps the clean water in the clean water collection chamber from the lower clean water channel 352 to the common channel 33, the rotor 6 rotates, aligns the common channel 33 with the circumferential hole 32 of the detection channel 38, and discharges the water in the common channel 33 through the detection channel 38 in sequence from the detection channel 38.

[0351] Optionally, the flow channel plate 3 is further provided with an air flow channel 36, and the inner end of the air flow channel 36 is communicated with another circumferential hole 32.

[0352] Before step S13, when the rotor 6 rotates and the transfer channel communicates with the detection channel 38 through one of the circumferential holes 32; the rotor 6 rotates so that the transfer channel aligns with the circumferential hole 32 communicated with the air, so that the common channel 33 communicates with the air flow channel 36, and the pump extracts air from the air flow channel 36 and enters the common channel 33.

[0353] Then the rotor 6 rotates, and the transfer channel communicates with the detection channel 38 through one of the circumferential holes 32; the pump drives the sample and reagent in the common channel 33 to enter the inlet of the mixing chamber 39 from the detection channel 38, enters the mixing curve channel 392 from the inlet of the mixing chamber 39 and then flows into the mixing chamber 391 to form a mixed liquid, and then drives the mixed liquid to flow into the detection channel 38 again from the outlet of the mixing chamber 39 and flows out from the detection channel 38.

[0354] The flow channel plate 3 of the present invention can also be adapted to other urine analyzers 100. By configuring the above-mentioned pump or rotor 6, the sample and reagent can be mixed in the flow channel plate 3 to reduce bubbles and increase the accuracy of detection.

[0355] The preferred embodiments of the present invention have been described in detail above. However, it should be understood that if necessary, aspects of the embodiments can be modified to adopt aspects, features, and concepts of various patents, applications, and publications to provide additional embodiments.

[0356] In view of the above detailed description, these and other changes can be made to the embodiments. Generally speaking, in the claims, the terms used should not be construed as being limited to the specific embodiments disclosed in the specification and claims, but should be understood to include all possible embodiments together with the full scope of equivalents enjoyed by these claims.

[0357] Those of ordinary skill in the art can understand that the above-described embodiments are specific embodiments for implementing the present invention. In actual applications, various changes can be made in form and details without departing from the spirit and scope of the present invention.

Claims

1. A method for using a microfluidic chip, characterized in that: The microfluidic chip includes a flow channel plate and a cover plate covering the front side of the flow channel plate: The front side of the flow channel plate is provided with: A center hole, the center hole passes through the flow channel plate; A plurality of circumferential holes, wherein the plurality of circumferential holes are arranged along a circumference formed with the central hole as the center; A common flow channel, the inner end of which is in communication with the central hole, and the outer end of which is connected to a pump via a driving pipe; A lower sample flow channel, the inner end of which is connected to one of the circumferential holes; the lower sample flow channel is connected to a collection chamber for holding a sample through a lower sample pipe, and a sample burette is provided on the lower sample pipe; an upper sample flow channel, the inner end of which is in communication with one of the circumferential holes and is in communication with the upper portion of the sample burette through an upper sample pipe; a reagent flow channel, the inner end of which is in communication with one of the circumferential holes; A detection flow channel, the inner end of which is connected to one of the circumferential holes; A mixing chamber, the mixing chamber is located on the path of the detection flow channel and has an outlet and an inlet, and the mixing chamber includes: A mixing bend, which is a curved flow channel and is located on the path of the detection flow channel; and At least one mixing chamber, wherein the width of the mixing chamber is greater than the diameter of the detection flow channel; The central hole is connected to a transfer channel of a rotor, and the outer end of the transfer channel can be connected to any one of the circumferential holes as the rotor rotates; Step S11, the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the upper sample flow channel, and the pump drives the driving pipeline to extract the air in the common flow channel, the transfer channel, the upper sample flow channel and the sample burette; Then the rotor rotates, and the outer end of the transfer channel is aligned with the circumferential hole connected to the lower sample flow channel, and the sample in the collection chamber is drawn from the lower sample flow channel to the common flow channel; Step S12, the rotor rotates, the transfer channel is connected to the reagent flow channel through one of the circumferential holes, the pump extracts the air in the common flow channel and the reagent flow channel, and draws the sample in the reagent flow channel into the common flow channel; In step S13, the rotor rotates, and the transfer channel is connected to the detection channel through one of the circumferential holes; the pump drives the sample and reagent in the common channel to enter the inlet of the mixing chamber from the detection channel, and then enter the mixing bend from the inlet of the mixing chamber and flow into the mixing chamber to form a mixed liquid, and then drives the mixed liquid to flow into the detection channel again from the outlet of the mixing chamber and flow out of the detection channel.

2. The method for using the microfluidic chip according to claim 1, characterized in that: The mixing chamber is located above the mixing bend, and the inlet of the mixing chamber is below the outlet of the mixing chamber; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter the mixing bend from the entrance of the mixing chamber, and then flow into the mixing chamber. Then the mixed liquid flows back from the mixing chamber into the mixing bend, and then the mixed liquid is driven to flow from the mixing bend into the mixing chamber. Finally, the mixed liquid is driven to enter the detection flow channel from the mixing chamber again and flow out from the detection flow channel.

3. The method for using the microfluidic chip according to claim 1, characterized in that: The mixing chamber is located above the mixing curve; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter the mixing curve from the entrance of the mixing chamber, and then flow into the mixing chamber. Then, the mixed liquid flows back from the mixing chamber into the mixing curve. After staying for a preset period of time, the mixed liquid is driven to flow from the mixing curve into the mixing chamber. Finally, the mixed liquid is driven from the mixing chamber to enter the detection flow channel again and flow out of the detection flow channel.

4. The method for using the microfluidic chip according to claim 1, characterized in that: The mixing cavity comprises a plurality of mixing bends and a plurality of mixing chambers, wherein the plurality of mixing bends and the plurality of mixing chambers are arranged at intervals and communicated with each other; In step S13, the sample and reagent in the common flow channel are driven to enter the inlet of the mixing chamber from the detection flow channel, enter the multiple mixing bends and multiple mixing chambers from the inlet of the mixing chamber, and finally drive the mixed liquid to flow out of the detection flow channel.

5. The method for using the microfluidic chip according to claim 4, characterized in that: Each of the mixing chambers has an inlet and an outlet, respectively, the inlet and outlet of each of the mixing chambers are respectively connected to the adjacent mixing bends, and the inlet of each of the mixing chambers is located above the outlet; In step S13, after the sample and reagent enter the mixing cavity, they flow from one of the mixing bends through an adjacent one of the inlets into the mixing chamber, and then flow from the outlet into another mixing bend. The sample and reagent flow continuously in multiple mixing chambers and multiple mixing bends to form the mixed liquid.

6. The method for using the microfluidic chip according to claim 4, characterized in that: The mixing bend is an S-shaped bend and includes at least two horizontal flow channels and at least one turning flow channel, and any one of the turning flow channels is located at both ends of the two horizontal flow channels; The horizontal flow channel extends in a horizontal direction, and the turning flow channel includes a curved inner arc and an outer arc. Both the inner arc and the outer arc are arc-shaped, and the inner diameter of the inner arc is smaller than the inner diameter of the outer arc.

7. The method for using the microfluidic chip according to claim 4, characterized in that: The inner wall of the mixing chamber includes a first curved surface and a second curved surface opposite to the first curved surface, and the first curved surface and the second curved surface are curved surfaces protruding toward the outside.

8. The method for using the microfluidic chip according to claim 6, characterized in that: Each of the mixing chambers is connected to the horizontal flow channels of the two mixing bends, and the two horizontal flow channels are radially symmetrically arranged at the top and bottom of the mixing chamber; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter one of the mixing bends from the entrance of the mixing chamber, and then flow into the mixing chamber, and then flow from the mixing chamber into another mixing bend to form a mixed liquid. After staying for a preset period of time, the mixed liquid flows back into the mixing chamber, and the mixed liquid is driven to be discharged from the mixing bend.

9. The method for using the microfluidic chip according to claim 1, characterized in that: The microfluidic chip is also provided with a clean water flow channel; The common flow channel is operably connected to the clean water flow channel; After step S13, step S14 is also included: driving the water in the clean water channel into the common channel, and then driving the water in the common channel into the detection channel, and then entering the mixing chamber from the inlet of the mixing chamber, and then entering the detection channel again from the outlet of the mixing chamber, and being discharged from the detection channel.

10. The method for using the microfluidic chip according to claim 1, characterized in that: The flow channel plate is also provided with a clean water flow channel, and the clean water flow channel is connected to one of the circumferential holes; After step S13, step S14 is also included: the rotor rotates, the transfer channel is connected with the clean water flow channel through one of the circumferential holes, the pump drives the water in the clean water flow channel to enter the common flow channel, and then drives the water in the common flow channel to enter the detection flow channel, and then enters the mixing chamber from the inlet of the mixing chamber, and then enters the detection flow channel again from the outlet of the mixing chamber, and is discharged from the detection flow channel.

11. The method for using the microfluidic chip according to claim 1, characterized in that: The flow channel plate is provided with an upper clean water flow channel and a lower clean water flow channel, and the inner end of the upper clean water flow channel and the inner end of the lower clean water flow channel are respectively connected to the two circumferential holes; The lower clean water flow channel is connected to the water collection chamber through the lower clean water pipe, and a clean water burette is provided on the lower clean water pipe; the upper clean water flow channel is connected to the upper part of the clean water burette through the upper clean water pipe; After step S13, step S14 is also included: the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the upper clean water flow channel, and the pump draws the air in the common flow channel, the transfer channel, the upper clean water flow channel and the clean water burette to the outside; then the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the lower clean water flow channel, and the clean water in the collection chamber is pumped from the lower clean water flow channel to the common flow channel, the rotor rotates to align the common flow channel with the circumferential hole of the detection flow channel, and the water in the common flow channel is discharged from the detection flow channel in turn.

12. The method for using the microfluidic chip according to claim 1, characterized in that: The flow channel plate is also provided with an air flow channel, the inner end of which is connected to another circumferential hole; Step S13, the rotor rotates, and before the transfer channel is connected to the detection channel through one of the circumferential holes; the rotor rotates so that the transfer channel is aligned with the circumferential hole connected to the air channel, so that the common channel is connected to the air channel, and the pump draws air from the air channel into the common channel; Then the rotor rotates, and the transfer channel is connected to the detection flow channel through one of the circumferential holes; the pump drives the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the mixing bend from the inlet of the mixing chamber, and then flow into the mixing chamber to form a mixed liquid, and then drives the mixed liquid to flow into the detection flow channel again from the outlet of the mixing chamber, and then flow out of the detection flow channel.

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