SSR (Simple Sequence Repeat) marker detection primer for identifying potato diploid and application of SSR marker detection primer

Abstract

The invention relates to the technical field of biology, in particular to an SSR (Simple Sequence Repeat) marker detection primer for identifying potato diploid and application thereof, which can be used for quickly and accurately identifying and analyzing ploidy and genome purity of a ploidy reduction group of 'Liughua No.6' and has important application value in the aspects of potato breeding parent selection and filial generation selection research.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to an SSR marker detection primer for potato diploid identification and a use thereof. Background Art

[0002] Potato is a general term for some species with edible tubers in the genus Solanum of the Solanacea family, native to the Andes Mountains in South America. At present, 235 potato species have been discovered, including 7 cultivated species including common cultivated species and original cultivated species, and 228 wild species. The ploidy of these species is also relatively complex, including diploid (such as S. phureja), triploid (such as S. juzepczukii), tetraploid (such as S. tuberosum) and pentaploid (such as S. curtilobum), and most of them are diploid. It is difficult to hybridize between materials with different ploidy. In order to develop and utilize the abundant diploid resources, it is necessary to reduce the ploidy of the tetraploid common cultivated species S. tuberosum to diploid.

[0003] The commonly used potato ploidy identification methods in scientific research include: (1) Plant morphology identification: Compared with materials with lower ploidy, materials with higher ploidy have thicker leaves, thicker stems, larger flowers and fruits, etc. However, the role of genes controlling various traits is not very clear at present, and there are many exceptions. This method can only be used as an auxiliary means of ploidy identification. (2) Chloroplast counting in stomatal guard cells: The number of chloroplasts in the stomatal guard cells of a species is basically constant, and its number usually increases with the increase of ploidy. Therefore, the ploidy of the species can be roughly determined by the number of chloroplasts in stomatal guard cells. (3) Flow cytometry: Flow cytometry can directly measure the DNA content of cells, thereby identifying the ploidy of plants. However, this method cannot currently determine aneuploidy in organisms. (4) Chromosome counting: The ploidy of organisms is related to the number of chromosomes in cells. Selecting cells in the middle and late stages of cell division to count chromosomes is the most intuitive and accurate method for ploidy identification. However, this method has low work efficiency and requires high experimental skills of personnel. (5) Molecular marker identification: Molecular markers are usually a special DNA sequence in the genome. By detecting the differences in these DNA sequences, they can be used as auxiliary evidence to determine the gene (group) origin and ploidy of the plant. (6) Whole genome sequencing: The whole genome of the experimental material is sequenced and assembled, and the ploidy is determined based on the assembled chromosome sequence. However, this method is expensive and requires very high skills of the operator.

[0004] Zheng Yingzhuan et al. conducted research on the identification of potato triploids, tetraploids and diploids using SSR markers in the potato "Hezuo 88" reduced ploidy population. The study used 7 pairs of SSR primers to analyze the relationship between diploids, triploids and tetraploids in the C88 reduced ploidy population. According to the SSR detection results, the ploidy misdetection rate of these 7 pairs of primers for the reduced ploidy population was 12.0%. These 7 pairs of primers were used to detect the reduced ploidy population of "Lishu No. 6", and it was found that the results were confusing and could not be used for ploidy detection of the parents of the population and the reduced ploidy population. Therefore, it is necessary to find SSR marker primers suitable for the "Lishu No. 6" reduced ploidy population for rapid and accurate identification of potato ploidy. Summary of the invention

[0005] In order to overcome the shortcomings of the above technical defects, the present invention provides an SSR marker detection primer for potato diploid identification and its use, which can be used to quickly identify the ploidy of the high-yield potato variety "Lishu No. 6" and its derivative materials.

[0006] To achieve the above object, the present invention is implemented through the following scheme:

[0007] The genome of the maternal variety "Lishu No. 6" was sequenced using the Illumina xplus sequencing platform and the PacBio sequencing platform, and the second-generation and third-generation sequencing results were assembled using SOAPdenovo and HifiAsm for whole-genome sequence splicing. The assembled "Lishu No. 6" genome was subjected to SSR scanning analysis, and primers were designed to extend 100-200bp on both wings of the obtained SSR fragments to obtain corresponding detection primers. PCR amplification was performed with detection primers in the "Lishu No. 6" reduced ploidy population, and the amplified products were detected by electrophoresis. Screening was performed based on the electrophoresis results, and the inventors determined 6 pairs of primers that can be used to distinguish the ploidy of the population in combination (screening process see Figure 1 ) can be used to quickly and accurately identify and analyze the ploidy and genomic purity of the "Lishu No. 6" reduced ploidy population.

[0008] Therefore, in a first aspect, the present invention provides an SSR marker detection primer for potato diploid identification, comprising a primer pair as shown below:

[0009] Primer pair A1: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively;

[0010] Primer pair A2: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 3 and SEQ ID NO: 4, respectively;

[0011] Primer pair A3: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 5 and SEQ ID NO: 6, respectively;

[0012] Primer pair B1: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 7 and SEQ ID NO: 8, respectively;

[0013] Primer pair G3: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 9 and SEQ ID NO: 10, respectively;

[0014] Primer pair G4: comprises a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 11 and SEQ ID NO: 12, respectively.

[0015] The present invention utilizes these 6 pairs of primers to detect the ploidy of the parents and reduced ploidy population of the potato tetraploid variety "Lishu No. 6", with an accuracy rate of up to 97.8%.

[0016] Further, the nucleotide sequence of the primer is (a), (b) or (c), achieving the same detection purpose;

[0017] (a) the nucleotide sequence shown in SEQ ID NO.1 to SEQ ID NO.12;

[0018] (b) a nucleotide sequence that hybridizes with and encodes the nucleotide sequence shown in SEQ ID NO.1 to SEQ ID NO.12 under stringent conditions;

[0019] (c) A nucleotide sequence encoding a nucleotide sequence having 80% or more homology to the nucleotide sequence shown in SEQ ID NO. 1 to SEQ ID NO. 12.

[0020] In some specific embodiments, the nucleotide sequence of the SSR marker detection primer pair provided by the present invention has 80% identity with the sequence shown in SEQID NO.1-SEQ ID NO.12; preferably, it has 85% identity, more preferably, it has 90% identity, more preferably, it has 95% identity, and most preferably, it has 99% identity.

[0021] Exemplarily, "stringent conditions" as described herein refer to conditions under which the probe will hybridize to its target sequence to a detectable extent exceeding hybridization with other sequences (e.g., at least 2 times the background). Stringent conditions are sequence-dependent and vary depending on the environment. By controlling the stringency of hybridization and / or washing conditions, a target sequence that is 100% complementary to the probe can be identified. Alternatively, stringent conditions can be adjusted to allow some sequence mismatches so that a lower degree of similarity is detected. These nucleotide sequences that hybridize under stringent conditions can be used, for example, to express variant proteins of SEQ ID NO.1 or can be used as primers, probes, exogenous donor sequences, guide RNA, antisense RNA, shRNA and siRNA.

[0022] In a second aspect, the present invention provides a kit for potato diploid identification, comprising the above primer pair set.

[0023] In a third aspect, the present invention provides use of a primer pair for detecting SSR markers in identifying the ploidy of potato distant hybridization progeny.

[0024] Furthermore, the potato is "Lishu No. 6".

[0025] Further, determining whether the sample to be tested includes the DNA fragments shown in (a)-(n);

[0026] (a) the nucleotide sequence shown in SEQ ID NO: 13;

[0027] (b) the nucleotide sequence shown in SEQ ID NO: 14;

[0028] (c) the nucleotide sequence shown in SEQ ID NO: 15;

[0029] (d) the nucleotide sequence shown in SEQ ID NO: 16;

[0030] (e) the nucleotide sequence shown in SEQ ID NO: 17;

[0031] (f) the nucleotide sequence shown in SEQ ID NO: 18;

[0032] (g) the nucleotide sequence shown in SEQ ID NO: 19;

[0033] (h) the nucleotide sequence shown in SEQ ID NO: 20;

[0034] (i) the nucleotide sequence shown in SEQ ID NO: 21;

[0035] (j) the nucleotide sequence shown in SEQ ID NO: 22;

[0036] (k) the nucleotide sequence shown in SEQ ID NO: 23;

[0037] (l) the nucleotide sequence shown in SEQ ID NO: 24;

[0038] (m) the nucleotide sequence shown in SEQ ID NO: 25;

[0039] (n) The nucleotide sequence shown in SEQ ID NO: 26.

[0040] In some specific embodiments, 6 pairs of primers (whose nucleotide sequences are shown in SEQ ID NO.1-SEQ ID NO.12) can amplify a total of 14 DNA fragments (whose nucleotide sequences are shown in SEQ ID NO.13-SEQ ID NO.26) in the mother plant. The presence or absence of these DNA fragments can be used to identify the ploidy of distant hybridization offspring.

[0041] In a third aspect, the present invention provides the use of an SSR marker detection primer pair in identifying the genomic purity of potato distant hybridization progeny.

[0042] Furthermore, the potato is "Lishu No. 6".

[0043] Furthermore, the uses include nucleic acid hybridization detection, molecular labeling, preparation of gene chips, preparation of molecular probes, and preparation of detection kits.

[0044] Further, determining whether the sample to be tested includes the DNA fragments shown in (a)-(i);

[0045] (a) the nucleotide sequence shown in SEQ ID NO: 27;

[0046] (b) the nucleotide sequence shown in SEQ ID NO: 28;

[0047] (c) the nucleotide sequence shown in SEQ ID NO: 29;

[0048] (d) the nucleotide sequence shown in SEQ ID NO: 30;

[0049] (e) the nucleotide sequence shown in SEQ ID NO: 31;

[0050] (f) the nucleotide sequence shown in SEQ ID NO: 32;

[0051] (g) the nucleotide sequence shown in SEQ ID NO: 33;

[0052] (h) the nucleotide sequence shown in SEQ ID NO: 34;

[0053] (i) The nucleotide sequence shown in SEQ ID NO:35.

[0054] In some specific embodiments, 6 pairs of primers (nucleotide sequences of which are shown in SEQ ID NO.1-SEQ ID NO.12) can amplify a total of 9 DNA fragments (nucleotide sequences of which are shown in SEQ ID NO.29-SEQ ID NO.36) in the paternal IVP101. Based on the presence or absence of these DNA fragments, it can be inferred whether there is introgression of paternal genes in the double materials, thereby identifying the genomic purity of the offspring of distant hybridization.

[0055] Furthermore, the potato is "Lishu No. 6".

[0056] Furthermore, the uses include nucleic acid hybridization detection, molecular labeling, preparation of gene chips, preparation of molecular probes, and preparation of detection kits.

[0057] Compared with the prior art, the present invention has the following beneficial effects:

[0058] (1) The amplification results of 6 pairs of SSR marker primers are used to determine the ploidy of the reduced population. Compared with genome sequencing and flow cytometry, it does not require special instruments and equipment, and the detection cost is also lower;

[0059] (2) Compared with methods such as embryo spot marking, plant morphology observation, and physiological and biochemical index measurement, the test results are more objective, more accurate, and have good repeatability. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] Figure 1 Flow chart for primer screening for SSR marker detection;

[0061] Figure 2 This is the ploidy detection diagram of the hybridization-induced ploidy reduction population material of "Lishu No. 6";

[0062] Figure 3 This is the agarose gel electrophoresis detection diagram of SSR marker primers;

[0063] Figure 4 This is a cluster analysis diagram of the SSR marker detection results of the hybridization-induced reduced polyploidy population of "Lishu No. 6". DETAILED DESCRIPTION

[0064] In order to make those skilled in the art better understand the technical scheme of the present invention, the present invention is described in detail below in conjunction with specific embodiments. The experimental methods for which specific conditions are not indicated in the following examples are usually carried out under normal conditions or according to the conditions recommended by the manufacturer. The test materials used in the following examples, unless otherwise specified, are purchased from conventional biochemical reagent stores. Unless otherwise specified, percentages and parts are calculated by weight. Unless otherwise defined, all professional and scientific terms used in the text have the same meaning as those familiar to those skilled in the art. In addition, any method and material similar to or equal to the recorded content can all be applied to the present invention. The preferred implementation methods and materials described in the text are only for demonstration purposes.

[0065] Based on the present invention, in order to develop the diploid resources of the tetraploid excellent potato variety "Lishu No. 6", "Lishu No. 6" was reduced in ploidy to obtain a reduced ploidy population including diploid, triploid and tetraploid materials. Therefore, it is very important to quickly and accurately identify the ploidy of potatoes.

[0066] The technical solution of the present invention is further described in detail below in conjunction with specific embodiments and drawings. It should be understood that the following embodiments are only used to explain the present invention, and are not used to limit the present invention.

[0067] Based on the present invention, in order to develop the diploid resources of the tetraploid excellent potato variety "Lishu No. 6", "Lishu No. 6" was reduced in ploidy to obtain a reduced ploidy population including diploid, triploid and tetraploid materials. After analyzing the genome of "Lishu No. 6", the inventor developed SSR marker primers suitable for the reduced ploidy population of "Lishu No. 6", which can be used to better judge the ploidy of the reduced ploidy population material and estimate the flow direction of parental genes.

[0068] Example 1

[0069] The tetraploid potato variety "Lishu No. 6" was subjected to a ploidy reduction treatment. The leaves of some of the obtained materials were cut, quickly frozen in liquid nitrogen and stored at -80°C. The ploidy of the reduced ploidy materials was determined by flow cytometry (see Figure 2 ),like Figure 2 As shown in the figure, a: Lishu No. 6 (tetraploid, female parent); b: IVP101 (diploid, male parent); c: diploid offspring; d: triploid offspring; e: tetraploid offspring.

[0070] Example 2

[0071] The second and third generation genome sequencing of the tetraploid potato variety "Lishu No. 6" was performed on the Illumina xplus sequencing platform and the PacBio sequencing platform, respectively. The second and third generation sequencing results were assembled using SOAPdenovo and HifiAsm.

[0072] The assembled "Lishu No. 6" genome was subjected to SSR scanning analysis, and the two wings of the obtained SSR fragments were extended by 100-200bp for primer design. First, the fragments with low GC% content at both ends of the fragments that could not be used to design amplification primers were removed; then, the amplified fragments were selected to be specific to a certain chromosome and different on different chromatids of the same chromosome; the expected amplified fragments were then compared with the published diploid potato Phureja DM 1-3 516R44 v8.1 genome, and the primers were used to perform ePCR on the genome, and the fragments and primers that can distinguish between the parents were retained; the screened and retained primers were subjected to PCR detection and verification in the "Lishu No. 6" reduced ploidy population, and the amplified products were detected by electrophoresis. According to the electrophoresis results, screening was performed to determine 6 pairs of SSR marker detection primers that can be used for ploidy screening of the reduced ploidy population, which are primer pair A1 (the nucleotide sequence of which is shown in SEQ ID NO: 1 and SEQ ID NO: 2), primer pair A2 (the nucleotide sequence of which is shown in SEQ ID NO: 3, SEQ ID NO: 4), and primer pair A3 (the nucleotide sequence of which is shown in SEQ ID NO: 5). IDNO: 4), primer pair A3 (whose nucleotide sequence is shown in SEQ ID NO: 5, SEQ ID NO: 6), primer pair B1 (whose nucleotide sequence is shown in SEQ ID NO: 7, SEQ ID NO: 8), primer pair G3 (whose nucleotide sequence is shown in SEQ ID NO: 9, SEQ ID NO: 10) and primer pair G4 (whose nucleotide sequence is shown in SEQ ID NO: 11, SEQ ID NO: 12).

[0073] Example 3

[0074] The modified CTAB method was used to extract the total DNA of "Lishu No. 6", IVP101 and leaves, and the designed 6 pairs of InDel marker detection primers were used for PCR amplification detection and verification in the two parents and the reduced ploidy populations identified as diploid, triploid and tetraploid by flow cytometry. The amplification system and amplification procedure are shown in Tables 1 and 2:

[0075] Table 1 Amplification system

[0076]

[0077] Table 2 Amplification procedures

[0078]

[0079] The amplified products were detected by electrophoresis on 2% agarose gel (see Figure 3 ), and found that 6 primer pairs could be used to distinguish between the parents. Figure 3 In the figure, M is Maker, L is LS6, I is IVP101, and the other lanes are the LS6×IVP101 down-progeny populations; Figure 3As can be seen in the figure, in the parent LS6, 6 pairs of designed primers can amplify 14 fragments in total, among which primer pair A1 can amplify 3 target fragments, namely A1-1 (whose nucleotide sequence is shown in SEQ ID NO: 13), A1-2 (whose nucleotide sequence is shown in SEQ ID NO: 14) and A1-3 (whose nucleotide sequence is shown in SEQ ID NO: 15), with lengths of 238 bp, 232 bp and 214 bp, respectively; primer pair A2 can also amplify 3 target fragments, namely A2-1 (whose nucleotide sequence is shown in SEQ ID NO: 16), A2-2 (whose nucleotide sequence is shown in SEQ ID NO: 17) and A2-3 (whose nucleotide sequence is shown in SEQ ID NO: 18), with lengths of 184 bp, 178 bp and 160 bp, respectively; primer pair A3 can amplify 2 target fragments, namely A3-1 (whose nucleotide sequence is shown in SEQ ID NO: 19), A3-2 (whose nucleotide sequence is shown in SEQ ID NO: 20) and A3-3 (whose nucleotide sequence is shown in SEQ ID NO: 21). NO: 20), with lengths of 207 bp and 196 bp respectively; primer pair B1 can amplify 3 target fragments, namely B1-1 (its nucleotide sequence is shown in SEQ ID NO: 21), B1-2 (its nucleotide sequence is shown in SEQ ID NO: 22) and B1-3 (its nucleotide sequence is shown in SEQ ID NO: 23), with lengths of 167 bp, 162 bp and 141 bp respectively; primer pair G3 can only amplify 1 target fragment B3-1 (its nucleotide sequence is shown in SEQ ID NO: 24), with a length of 232 bp; primer pair G4 can amplify 2 target fragments, namely G4-1 (its nucleotide sequence is shown in SEQ ID NO: 25) and G4-2 (its nucleotide sequence is shown in SEQ ID NO: 26), with lengths of 343 bp and 298 bp respectively. According to the presence or absence of these DNA fragments, the ploidy of distant hybridization offspring can be identified.

[0080] Furthermore, it was found that in the parent IVP101, 6 pairs of designed primers could amplify a total of 9 fragments. Among them, primer pair A1 amplified 1 target fragment IVP101-A1-1 (its nucleotide sequence is shown in SEQ ID NO: 27), with a length of 226 bp; primer pair A2 amplified 1 target fragment IVP101-A2-1 (its nucleotide sequence is shown in SEQ ID NO: 28), with a length of 172 bp; primer pair G3 amplified 1 target fragment IVP101-G3-1 (its nucleotide sequence is shown in SEQ ID NO: 29), with a length of 214 bp; primer pair A3 amplified 2 target fragments IVP101-A3-1 (its nucleotide sequence is shown in SEQ ID NO: 30) and IVP101-A3-2 (its nucleotide sequence is shown in SEQ ID NO: 31), with lengths of 199 bp and 198 bp, respectively; primer pair B1 amplified 2 target fragments IVP101-B1-1 (its nucleotide sequence is shown in SEQ ID NO: 32), IVP101-B1-2 (its nucleotide sequence is shown in SEQ ID NO: 33), with lengths of 148bp and 134bp respectively; two target fragments amplified from G4 are IVP101-G4-1 (its nucleotide sequence is shown in SEQ ID NO: 34) and IVP101-G4-2 (its nucleotide sequence is shown in SEQ ID NO: 35), with lengths of 331bp and 325bp respectively; based on the presence or absence of these fragments, it can be inferred whether the paternal gene has been infiltrated into the reduced-fold material.

[0081] The ploidy analysis was performed based on the PCR test results. The test results of 6 pairs of SSR marker primers in 269 "Lishu 6" reduced ploidy population materials were marked, and the results were clustered using the UPGMA (Unweighted Pair-Group Method with Arithmetic means) method (see Figure 4 ).like Figure 4 As shown, the yellow shading part represents LS6 (maternal parent), the red shading part represents IVP101 (paternal parent), the green shading part represents diploid, and the purple shading part represents tri-tetraploid. The results showed that 2 diploid materials out of 188 diploid materials were classified as tri-tetraploid materials, and 4 tetraploid materials were classified as diploid materials, with an accuracy rate of 97.8%.

[0082] Finally, it should be noted that the above description is only a preferred embodiment of the present invention. Under the guidance of the present invention, those skilled in the art can make a variety of similar expressions without violating the purpose and claims of the present invention, and such changes all fall within the protection scope of the present invention.

Claims

1. A SSR marker detection primer for potato diploid identification, characterized in that Include the following primer pairs: Primer pair A1: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 1 and SEQ ID NO: 2, respectively; Primer pair A2: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 3 and SEQ ID NO: 4, respectively; Primer pair A3: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 5 and SEQ ID NO: 6, respectively; Primer pair B1: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 7 and SEQ ID NO: 8, respectively; Primer pair G3: comprising a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 9 and SEQ ID NO: 10, respectively; Primer pair G4: comprises a forward primer and a reverse primer whose nucleotide sequences are shown in SEQ ID NO: 11 and SEQ ID NO: 12, respectively.

2. The primer according to claim 1, characterized in that: The nucleotide sequence of the primer is (a), (b) or (c); (a) the nucleotide sequence shown in SEQ ID NO.1 to SEQ ID NO.12; (b) a nucleotide sequence that hybridizes with and encodes the nucleotide sequence shown in SEQ ID NO.1 to SEQ ID NO.12; (c) A nucleotide sequence encoding a nucleotide sequence having 80% or more homology to the nucleotide sequence shown in SEQ ID NO. 1 to SEQ ID NO.

12.

3. Use of the primer set according to claim 1 or 2 in identifying the ploidy of potato distant hybridization progeny.

4. The use according to claim 3, characterized in that: Determine whether the sample to be tested includes the DNA fragments shown in (a)-(n); (a) the nucleotide sequence shown in SEQ ID NO: 13; (b) the nucleotide sequence shown in SEQ ID NO: 14; (c) the nucleotide sequence shown in SEQ ID NO: 15; (d) the nucleotide sequence shown in SEQ ID NO: 16; (e) the nucleotide sequence shown in SEQ ID NO: 17; (f) the nucleotide sequence shown in SEQ ID NO: 18; (g) the nucleotide sequence shown in SEQ ID NO: 19; (h) the nucleotide sequence shown in SEQ ID NO: 20; (i) the nucleotide sequence shown in SEQ ID NO: 21; (j) the nucleotide sequence shown in SEQ ID NO: 22; (k) the nucleotide sequence shown in SEQ ID NO: 23; (l) the nucleotide sequence shown in SEQ ID NO: 24; (m) the nucleotide sequence shown in SEQ ID NO: 25; (n) The nucleotide sequence shown in SEQ ID NO:

26.

5. Use of the primer set according to claim 1 or 2 in identifying the genomic purity of potato distant hybridization offspring.

6. The use according to claim 5, characterized in that: Determine whether the sample to be tested includes the DNA fragments shown in (a)-(i); (a) the nucleotide sequence shown in SEQ ID NO: 27; (b) the nucleotide sequence shown in SEQ ID NO: 28; (c) the nucleotide sequence shown in SEQ ID NO: 29; (d) the nucleotide sequence shown in SEQ ID NO: 30; (e) the nucleotide sequence shown in SEQ ID NO: 31; (f) the nucleotide sequence shown in SEQ ID NO: 32; (g) the nucleotide sequence shown in SEQ ID NO: 33; (h) the nucleotide sequence shown in SEQ ID NO: 34; (i) The nucleotide sequence shown in SEQ ID NO:

35.

7. The use according to claim 3 or 4, characterized in that: The potato is "Lishu No. 6".

8. The use according to claim 5 or 6, characterized in that: The potato is "Lishu No. 6".

9. The use according to claim 3 or 4, characterized in that: The uses include nucleic acid hybridization detection, molecular labeling, preparation of gene chips, preparation of molecular probes, and preparation of detection kits.

10. The use according to claim 5 or 6, characterized in that: The uses include nucleic acid hybridization detection, molecular labeling, preparation of gene chips, preparation of molecular probes, and preparation of detection kits.

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