Tumor liquid biopsy kit based on microfluidic technology

Abstract

The invention relates to the technical field of biology, and discloses a tumor liquid biopsy kit based on a microfluidic technology, the tumor liquid biopsy kit comprises a detection main body, the upper side of the detection main body is provided with a biopsy kit main body, the upper side of the detection main body is provided with a filtering mechanism, and the rear side of the detection main body is provided with a placing mechanism; a heating mechanism is arranged on the inner side of the detection main body, and a plurality of groups of buckling mechanisms are arranged on the right side of the detection main body; the filtering mechanism comprises a stand column, the stand column is arranged on the upper side of the detection body, a plurality of annular grooves are formed in the outer side of the stand column, and four clamping grooves are formed in the lower sides of the annular grooves at equal intervals. Blood impurities and thrombus can be removed through a multi-layer filter by filtering, sample components can be fully mixed by stirring, a reliable result is guaranteed, a reagent bottle placed in a folding manner is convenient to carry, a silica gel cylinder can be stored, and a heating mechanism consists of a controller and a heating pipe, so that the temperature requirement of detection is met, and the process and the result are guaranteed.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a tumor liquid biopsy kit based on microfluidics technology. Background Art

[0002] Tumor diagnosis faces many challenges. Traditional imaging examinations have low sensitivity for early tumors. Although tissue biopsy is the gold standard, it is invasive and has problems such as sampling errors. Liquid biopsy technology has emerged because it can detect tumor components in body fluids, but traditional detection methods are difficult to meet its high sensitivity and specificity requirements. Microfluidic technology has developed accordingly. It can accurately control flow in micro-nano channels and has obvious advantages in liquid biopsy. It requires less sample volume, has high sensitivity, and is fast to analyze. It can also be integrated and automated, bringing new hope to tumor diagnosis.

[0003] First, a blood sample is collected and introduced into the test kit, and pre-processed in or before the microfluidic chip, such as filtering impurities and lysing cells. Then, tumor markers are enriched. Circulating tumor cells can be captured by structures such as microcolumn arrays, and circulating tumors are detected using aptamers and probe technology. Optical, electrochemical or other detection methods are then used, such as fluorescent labeling optical detection and electrochemical sensor detection. Finally, the results are output after signal processing, and doctors can diagnose tumors, evaluate therapeutic effects and predict the risk of recurrence based on this.

[0004] When testing blood, if the blood concentration is high, it needs to be stirred or diluted first. If there is a blood clot in the blood, it is very easy to block the device and affect the normal progress of the test. In addition, when it is necessary to go out for testing, you have to carry a reagent bottle rack, which undoubtedly increases the burden of going out for testing and brings many inconveniences to the testing work. Summary of the invention

[0005] In view of the deficiencies in the prior art, the present invention provides a tumor liquid biopsy kit based on microfluidic technology, which solves the problems of high-concentration blood needing to be stirred or diluted, blood clots easily blocking the device, and the need to carry a reagent bottle rack when going out for testing, which increases the burden and inconvenience.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: a tumor liquid biopsy kit based on microfluidic technology, comprising a detection body, a biopsy kit body is arranged on the upper side of the detection body, a filtering mechanism is arranged on the upper side of the detection body, a placing mechanism is arranged on the rear side of the detection body, a heating mechanism is arranged on the inner side of the detection body, and multiple groups of buckle mechanisms are arranged on the right side of the detection body; The filtering mechanism includes a column, which is arranged on the upper side of the detection body, and a plurality of annular grooves are provided on the outer side of the column, and four slots are equidistantly provided on the lower side of the annular groove, and a ring block is provided outside the annular groove, and two slots are equidistantly provided on the inner side of the ring block, and the slots are engaged with the corresponding slots, and the outer sides of the ring blocks are respectively connected to U-shaped plate one and U-shaped plate two through connecting blocks, and a stirring assembly is provided on the inner side of the U-shaped plate one, and a stainless steel mesh pre-filter, a silicon nitride membrane intermediate filter and a fine ceramic membrane filter are respectively provided on the inner side of the U-shaped plate two.

[0007] Preferably, the placement mechanism includes a placement plate, which is connected to the rear side of the detection body through a hinge, a plurality of circular holes are provided on the upper side of the placement plate, a silicone tube is provided on the inner side of the circular holes, a square groove is provided on both sides of the placement plate, and an adjustment component is provided on the inner side of the square groove.

[0008] Preferably, the heating mechanism comprises a controller, the controller is arranged on the right side of the detection body, and a heating tube is arranged at the output end of the controller, and the heating tube is arranged on the inner side of the detection body.

[0009] Preferably, the snap mechanism includes square boxes, which are equidistantly arranged on the right side of the detection body, wherein sliders are slid inside the square boxes, springs are arranged between the sliders and the inner walls of the square boxes, slide plates are arranged on the outer sides of the sliders, and triangular blocks are arranged on the tops of the sliders.

[0010] Preferably, the stirring assembly comprises a motor, the motor is arranged on the lower side of the U-shaped plate 1, and a stirring blade is arranged at the output end of the motor.

[0011] Preferably, the adjustment assembly includes a pull rod, and the pull rod slides on the inner side of the square groove one. The pull rod is connected to the square groove one through a spring two, and the pull rod is engaged with the cylindrical hole corresponding to the detection body.

[0012] Preferably, a square groove 2 is provided on the upper side of the detection body, a slide groove is provided on the inner wall of the square groove 2, a biopsy kit body is provided on the inner side of the square groove 2, and a long strip is provided on the outer side of the biopsy kit body, and the long strip slides on the inner side of the slide groove.

[0013] Preferably, the fine hole at the bottom of the U-shaped plate 1 is close to the upper side of the U-shaped plate 2, and the fine ceramic membrane filter at the bottom of the U-shaped plate 2 is close to the drip inlet of the biopsy kit body.

[0014] Preferably, a reagent bottle is arranged inside the silica gel cylinder.

[0015] Preferably, bases are provided at the four corners of the bottom of the detection body.

[0016] The present invention provides a tumor liquid biopsy kit based on microfluidics technology. It has the following beneficial effects: 1. The present invention can effectively remove impurities and thrombi in blood samples through a multi-layer filtering structure such as a stainless steel mesh pre-filter, a silicon nitride membrane intermediate filter and a fine ceramic membrane filter, thereby ensuring sample purity, improving the accuracy of tumor liquid biopsy, providing high-quality samples for subsequent detection links, and reducing the interference of impurities on the test results; the motor drives the stirring blade to stir the sample to fully mix the sample components and ensure that the sample is in a uniform state before testing. This is particularly critical for the mixing operation of thick blood or after adding reagents, which is beneficial to improving the reliability of the test results.

[0017] 2. The placement plate of the present invention is connected to the detection body by a hinge and can be folded. At the same time, the silicone tube in the round hole can stably place the reagent bottle. This design is convenient for placing the reagent bottle when in use, and can save space when not in use or when carried out, thereby improving the portability and practicality of the test kit.

[0018] 3. The controller in the heating mechanism of the present invention can accurately control the heating tube to heat the relevant parts of the test kit, which can meet the temperature requirements of certain detection steps, such as ensuring the activity of biological components in the sample at a specific temperature and promoting certain reactions, thereby ensuring the smooth progress of the detection process and the accuracy of the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is an overall stereogram of the present invention; Figure 2 It is a rear view schematic diagram of the present invention; Figure 3 It is a schematic diagram of the development of the present invention; Figure 4 It is a schematic diagram of the unfolded rear view of the present invention; Figure 5 For the present invention Figure 1 An enlarged schematic diagram of the filtering mechanism; Figure 6 For the present invention Figure 1 An enlarged schematic diagram of the buckle mechanism; Figure 7 For the present invention Figure 1 An enlarged schematic diagram of the placement mechanism; Figure 8 For the present invention Figure 1 Enlarged schematic diagram of the heating mechanism.

[0020] Among them, 1. detection body; 2. filtering mechanism; 201. column; 202. ring groove; 203. card slot; 204. ring block; 205. card block; 206. connection block; 207. U-shaped plate 1; 208. motor; 209. stirring blade; 2010. stainless steel mesh pre-filter; 2011. silicon nitride membrane intermediate filter; 2012. fine ceramic membrane filter; 2013. U-shaped plate 2; 3. placement mechanism; 301. Placement plate; 302, round hole; 303, square slot one; 304, silicone tube; 4, snap mechanism; 401, square box; 402, spring one; 403, slider; 404, triangular block; 405, slide plate; 5, heating mechanism; 501, heating tube; 502, controller; 6, reagent bottle; 7, pull rod; 8, biopsy kit body; 9, square slot two; 10, slide groove; 11, long strip; 12, spring two; 13, base. DETAILED DESCRIPTION

[0021] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Example: Please see attached Figure 1 , Attachment Figure 2 , Attachment Figure 3 , Attachment Figure 4 , Attachment Figure 5 With attached Figure 6 The embodiment of the present invention provides a tumor liquid biopsy kit based on microfluidic technology, including a detection body 1, which is the core structure of the entire kit and is used to carry and cooperate with other components. A biopsy kit body 8 is arranged on the upper side of the detection body 1. The biopsy kit body 8 is used to accommodate and process samples and other related operations, and cooperates with the detection body 1 to complete the detection function. A square groove 9 is opened on the upper side of the detection body 1, and the square groove 9 provides space for placing the biopsy kit body 8 on the detection body 1. A sliding groove 10 is opened on the inner wall of the square groove 9. The slide groove 10 is used to cooperate with the long strip 11 on the outside of the biopsy kit body 8, so that the biopsy kit body 8 can slide stably and smoothly in the square groove 29. The biopsy kit body 8 is arranged on the inner side of the square groove 29, and the long strip 11 is arranged on the outer side of the biopsy kit body 8. The long strip 11 slides on the inner side of the slide groove 10. This sliding connection method is convenient for the installation, disassembly and operation of the biopsy kit body 8. The four corners of the bottom of the detection body 1 are provided with a base 13. The base 13 plays a role in supporting the entire detection body 1 to ensure the stability of the test kit during use. A filter mechanism 2 is provided on the upper side of the detection body 1. The filter mechanism 2 is used to filter the sample and remove the thrombus to ensure the accuracy of the detection. The filter mechanism 2 includes a column 201. The column 201 is an important supporting structure of the filter mechanism 2 and provides a basis for the installation and fixation of subsequent filter-related components. The outer side of the column 201 is provided with a plurality of annular grooves 202. The annular grooves 202 provide positioning and installation positions for installing and fixing other components. Four card slots 203 are evenly spaced on the lower side of the annular groove 202. The card slots 203 cooperate with other components to achieve the card-fitting fixation of the components and ensure the stable connection of the various components of the filter mechanism 2. A ring block 204 is provided on the outside of the annular groove 202. The ring block 204 is used as a connecting component to connect other components to the column 2. 01 are connected, two card blocks 205 are equidistantly arranged on the inner side of the ring block 204, and the card blocks 205 are all engaged with the corresponding card slots 203. This engaging structure ensures that the ring block 204 is stably fixed on the column 201, and the outer side of the ring block 204 is respectively connected to the U-shaped plate 1 207 and the U-shaped plate 2013 through the connecting block 206. The inner side of the U-shaped plate 1 207 is provided with a stirring component, and the stirring component is used to stir the sample to fully mix and dilute the sample components. The inner side of the U-shaped plate 2013 is respectively provided with a stainless steel mesh pre-filter 2010, a silicon nitride membrane intermediate filter 2011 and a fine ceramic membrane filter 2012. These different types of filters filter the sample step by step to remove impurities and thrombi of different sizes, etc. The stirring assembly includes a motor 208, which is the power source of the stirring assembly and provides power for the stirring operation. The motor 208 is arranged on the lower side of the U-shaped plate 1 207 and can directly drive the stirring blade 209. The output end of the motor 208 is provided with a stirring blade 209, and the stirring blade 209 rotates under the drive of the motor 208 to stir the sample. The fine holes at the bottom of the U-shaped plate 1 207 are close to the upper side of the U-shaped plate 2 2013, ensuring that the stirred sample can flow smoothly from the U-shaped plate 1 207 to the U-shaped plate 2 2013. The fine ceramic membrane filter 2012 at the bottom of the U-shaped plate 2 2013 is close to the dripping inlet of the biopsy kit body 8, so that the filtered sample can be accurately dripped into the biopsy kit body 8. A plurality of buckle mechanisms 4 are arranged on the right side of the detection body 1. The buckle mechanisms 4 are used to fix and limit the biopsy kit body 8 and other components to ensure the stability and safety of the kit. The buckle mechanism 4 includes a square box 401. The square box 401 is the outer shell of the buckle mechanism 4 and provides installation space for internal components. The square boxes 401 are equidistantly arranged on the right side of the detection body 1 to ensure that the buckles are evenly stressed. Slide blocks 403 are slid inside the square boxes 401. The sliders 403 are key moving components for realizing the buckle function. Springs 402 are arranged between the sliders 403 and the inner walls of the square boxes 401. The springs 402 provide elastic force for the sliders 403 to realize automatic resetting and stable fixation of the buckles. Slide plates 405 are arranged on the outside of the sliders 403. The slide plates 405 are convenient for users to operate the sliders 403. Triangular blocks 404 are arranged on the tops of the sliders 403. The triangular blocks 404 are used to buckle and limit the components.

[0023] Please see attached Figure 1 , Attachment Figure 2 , Attachment Figure 3 , Attachment Figure 7 With attached Figure 8 A placing mechanism 3 is provided at the rear side of the detection body 1. The placing mechanism 3 is used to place items such as reagent bottles 6 without affecting the operation of other components. The placing mechanism 3 includes a placing plate 301. The placing plate 301 is connected to the rear side of the detection body 1 through a hinge. The hinge can realize the rotation of the placing plate 301 relative to the detection body 1. A plurality of circular holes 302 are provided on the upper side of the placing plate 301. The circular holes 302 are used to place reagent bottles 6. Silicone tubes 304 are provided on the inner sides of the circular holes 302. The silicone tubes 304 protect and hold the reagent bottles 6. Square grooves 303 are provided on both sides of the placing plate 301. The square grooves 303 provide space for the installation of the adjustment component. The inside of the silicone tube 304 is provided with a reagent bottle 6. The reagent bottle 6 is placed in the silicone tube 304. An adjustment component is provided inside the square groove 1 303, and the adjustment component is used to adjust the parameters of the placement mechanism 3. The adjustment component includes a pull rod 7, and the pull rod 7 slides inside the square groove 1 303 to trigger the change of related functions of the placement mechanism 3. The pull rod 7 is connected to the square groove 1 303 through a spring 2 12, and the spring 2 12 provides elastic force for the pull rod 7 so that it can automatically reset. The pull rod 7 is engaged with the cylindrical hole corresponding to the detection body 1. This engagement method connects the placement mechanism 3 with the detection body 1 to ensure its stability and position accuracy. A heating mechanism 5 is provided on the inner side of the detection body 1, and the heating mechanism 5 is used to heat the sample or the reagent kit part. The heating mechanism 5 includes a controller 502. The controller 502 serves as the control core of the heating mechanism 5, which is convenient for users to operate and set parameters. A heating tube 501 is provided at the output end of the controller 502. The heating tube 501 is the heating execution component. The heating tube 501 is provided on the inner side of the detection body 1, and can directly heat the sample or related components to ensure the heating effect and avoid harm to the user.

[0024] Working principle: First, push the biopsy kit body 8 into the square slot 2 9. At this time, the triangular block 404 of the buckle mechanism 4 will block the biopsy kit body 8. Then, lift the placement plate 301 to make the pull rod 7 engage with the cylindrical hole of the detection body 1. Then, insert the reagent bottle 6 into the circular hole 302 and lead out the silicone tube 304. Then, lift the ring block 204 upward to disengage it from the slot 203. After rotating the ring block 204, engage it with the corresponding slot 203 to align the filter mechanism 2. Pick up the reagent bottle 6 and use a tool to drip blood into the U-shaped plate 1 207. If the blood is thick, pour in the reagent. The stirring blade 209 is driven by the motor 208 to stir. The blood flows from the liquid outlet at the bottom of the U-shaped plate 1 207 to the U-shaped plate Second 2013, the thrombus is filtered out through three filter layers including the stainless steel mesh pre-filter 2010, the silicon nitride membrane intermediate filter 2011 and the fine ceramic membrane filter 2012, and the value is checked on the display screen of the detection body 1. When heating is required, the controller 502 is used to control the heating tube 501 to open, and the bottom of the biopsy kit body 8 is heated. After the test is completed, the slide plate 405 is slid to drive the slider 403 with the triangular block 404 to slide, so that the triangular block 404 is separated from the biopsy kit body 8, and the biopsy kit body 8 is taken out, and an empty box is placed under the filter mechanism 2, and the filter mechanism 2 is disinfected with disinfectant, and the triangular block 404 is plugged into the round hole 302, and finally the pull rod 7 is pulled to fold the placement mechanism 3.

[0025] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A tumor liquid biopsy kit based on microfluidics technology, comprising a detection body (1), characterized in that: A biopsy kit body (8) is arranged on the upper side of the detection body (1), a filtering mechanism (2) is arranged on the upper side of the detection body (1), a placing mechanism (3) is arranged on the rear side of the detection body (1), a heating mechanism (5) is arranged on the inner side of the detection body (1), and a plurality of buckle mechanisms (4) are arranged on the right side of the detection body (1); The filtering mechanism (2) comprises a column (201), the column (201) being arranged on the upper side of the detection body (1), a plurality of annular grooves (202) being provided on the outer side of the column (201), four clamping grooves (203) being provided at equal distances on the lower side of the annular groove (202), a ring block (204) being provided on the outer side of the annular groove (202), two clamping blocks (205) being provided on the inner side of the annular block (204), and the clamping blocks (203) being provided at equal distances. 5) are engaged with the corresponding slots (203), the outer sides of the ring blocks (204) are respectively connected to the U-shaped plate 1 (207) and the U-shaped plate 2 (2013) through the connecting blocks (206), the inner side of the U-shaped plate 1 (207) is provided with a stirring assembly, and the inner side of the U-shaped plate 2 (2013) is respectively provided with a stainless steel mesh pre-filter (2010), a silicon nitride membrane intermediate filter (2011) and a fine ceramic membrane filter (2012).

2. The tumor liquid biopsy kit based on microfluidics technology according to claim 1, characterized in that: The placement mechanism (3) comprises a placement plate (301), the placement plate (301) being connected to the rear side of the detection body (1) via a hinge, a plurality of circular holes (302) being provided on the upper side of the placement plate (301), a silicone tube (304) being provided on the inner side of each of the circular holes (302), a square groove (303) being provided on both sides of the placement plate (301), an adjustment component being provided on the inner side of the square groove (303).

3. The tumor liquid biopsy kit based on microfluidics technology according to claim 1, characterized in that: The heating mechanism (5) comprises a controller (502), the controller (502) being arranged on the right side of the detection body (1), and a heating tube (501) being arranged at the output end of the controller (502), the heating tube (501) being arranged on the inner side of the detection body (1).

4. The tumor liquid biopsy kit based on microfluidics technology according to claim 1, characterized in that: The buckle mechanism (4) comprises a square box (401), the square boxes (401) are arranged at equal distances on the right side of the detection body (1), a slider (403) is slidably arranged inside the square box (401), a spring (402) is arranged between the slider (403) and the inner wall of the square box (401), a sliding plate (405) is arranged on the outer side of the slider (403), and a triangular block (404) is arranged on the top of the slider (403).

5. The tumor liquid biopsy kit based on microfluidics technology according to claim 1, characterized in that: The stirring assembly comprises a motor (208), the motor (208) being arranged on the lower side of the U-shaped plate 1 (207), and a stirring blade (209) being arranged at the output end of the motor (208).

6. The tumor liquid biopsy kit based on microfluidics technology according to claim 2, characterized in that: The adjustment assembly comprises a pull rod (7), and the pull rod (7) slides on the inner side of the square groove (303). The pull rod (7) is connected to the square groove (303) via the spring (12), and the pull rod (7) is engaged with the corresponding cylindrical hole of the detection body (1).

7. The tumor liquid biopsy kit based on microfluidics technology according to claim 1, characterized in that: A second square groove (9) is provided on the upper side of the detection body (1), a slide groove (10) is provided on the inner wall of the second square groove (9), a biopsy kit body (8) is provided on the inner side of the second square groove (9), and a long strip (11) is provided on the outer side of the biopsy kit body (8), and the long strip (11) slides on the inner side of the slide groove (10).

8. The tumor liquid biopsy kit based on microfluidics technology according to claim 1, characterized in that: The fine hole at the bottom of the U-shaped plate 1 (207) is close to the upper side of the U-shaped plate 2 (2013), and the fine ceramic membrane filter (2012) at the bottom of the U-shaped plate 2 (2013) is close to the dripping inlet of the biopsy kit body (8).

9. The tumor liquid biopsy kit based on microfluidics technology according to claim 2, characterized in that: A reagent bottle (6) is arranged inside the silica gel cylinder (304).

10. The tumor liquid biopsy kit based on microfluidics technology according to claim 1, characterized in that: Bases (13) are provided at the four corners of the bottom of the detection body (1).

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