Experimental equipment for rice pesticide residue detection
By designing an operating table with a detection table, a reaction table and a sample table, and equipped with a sample rotation assembly and a timing unit, the problem of cumbersome detection process and mistaken pick-up of existing equipment is solved, and efficient and accurate detection results are achieved.
Patent Information
- Application Number
- CN202510079005.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-17
- Publication Date
- 2025-04-25
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing rice pesticide residue detection equipment lacks an integrated operating platform, which leads to cumbersome and inefficient sample detection process, and is prone to mistaken pickups, affecting the detection results.
An operating table including a detection table, a reaction table and a sample table is designed. The holes and slots of each part are arranged one by one, and are equipped with a sample rotation assembly, a timing unit and a control motherboard. The samples are mixed by a motor driven by a rotary tube, the timing module controls the detection time, and prompts the operator through a signal light.
The sample detection process is achieved in an orderly and efficient manner, reducing the possibility of human operation errors, improving the accuracy and convenience of detection, and suitable for use in a fast on-site testing environment outside the laboratory.
Smart Images
Figure CN119881294A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of grain detection, in particular to an experimental device for detecting pesticide residues in rice. Background Art
[0002] In the production process of agricultural products such as fruits, vegetables, and grains, organophosphorus chemicals are widely used. Taking an organophosphate insecticide, chlorpyrifos, as an example, it is a broad-spectrum insecticide and acaricide, suitable for controlling pests and mites on various crops such as citrus, cotton, corn, apples, pears, rice, peanuts, soybeans, wheat, and tea trees. An existing chlorpyrifos detection kit adopts the principle of colloidal gold immunocompetitive inhibition chromatography. The kit mainly includes a pesticide residue extract, a gold-labeled microwell with a reaction substrate, and a detection card. The following steps are performed: 1. Weigh 1g of the sample to be tested in a centrifuge tube, add 3ml of the pesticide residue extract, and mix thoroughly for 3min to obtain the test solution to be tested; 2. Absorb 100uL of the test solution into the gold-labeled microwell, dissolve the reaction substrate in the well, and wait for the reaction for 5 minutes; 3. Absorb all the solution in the well and drop it into the sample addition well of the detection card. Start timing after adding the sample, and read the test result after 5-8 minutes. The existing test kits require a pipette and multiple test tubes to complete the operation during use, and multiple pipetting is required during the test, but there is currently no supporting integrated equipment for operation. When there are multiple samples to be tested, the large number of centrifuge tubes, gold-labeled microwells, and test cards will occupy a large area of the laboratory table, causing chaos on the laboratory table. When multiple people collaborate on the test, it is easy to take the wrong thing, which seriously affects the test efficiency. Therefore, it is necessary to make improvements. Summary of the invention
[0003] The purpose of the present invention is to provide an experimental device for detecting pesticide residues in rice. The device has a simple structure and is easy to use. It can be well applied to the colloidal gold method for detecting pesticide residues, and is very suitable for on-site rapid detection in environments outside laboratories such as market supervision, agricultural planting, farmers' markets, etc.
[0004] To achieve the above object, the present invention provides the following technical solutions:
[0005] An experimental device for detecting pesticide residues in rice, comprising an operating table, wherein the operating table is provided with a detection table portion, a reaction table portion and a sample table portion in sequence from front to back, wherein the top surface of the detection table portion is provided with one or more detection slots arranged in sequence from left to right, wherein the reaction table portion is higher than the detection table portion and has one or more reaction wells arranged in sequence from left to right on its top surface, wherein the sample table portion is higher than the reaction table portion and has one or more sample wells arranged in sequence from left to right on its top surface, wherein the detection slots, reaction wells and sample wells are arranged in one-to-one correspondence;
[0006] A sample rotating assembly is provided on the sample platform corresponding to each sample hole position, and the sample rotating assembly includes a support frame, a motor, a rotating tube and a rolling bearing. The support frame is fixedly installed in the sample platform, and the rotating tube and the motor are arranged in the support frame from top to bottom. The end of the output shaft of the motor is fixedly connected to the bottom of the rotating tube, and the support frame and the rotating tube are rotatably connected through the rolling bearing, and then the rotating tube is driven to rotate in the support frame by the motor, and the tube hole of the rotating tube forms a sample hole;
[0007] A timing unit is installed on the top surface of the detection platform corresponding to each detection slot, and the timing unit has a timing switch and a signal light.
[0008] Optimization scheme, the present invention also includes a control mainboard, on which a motor control module is provided for each motor of the sample rotating assembly, and a timing control module is provided for each timing unit;
[0009] The motor control module is used to control the running state of the motor;
[0010] The timing control module is used to control the timing state of the timing unit, and the timing control module includes a first timing module, a second timing module and a trigger counting module; the first timing module is used to time the first timing duration, the second timing module is used to time the second timing duration, and the trigger counting module is used to record the number of triggering times of the timing switch;
[0011] The motor of the sample rotating assembly, the timing switch and the signal light of the timing unit are electrically connected to the control mainboard respectively;
[0012] After the timing switch is triggered for the first time, the first timing module starts timing and the signal light goes off;
[0013] After the first timing module completes the timing, the signal light turns on and flashes;
[0014] After the timing switch is triggered for the second time, the second timing module starts timing and the signal light goes out at the same time;
[0015] After the second timing module finishes timing, the signal light lights up again and flashes;
[0016] After the timing switch is triggered for the third time, the signal light goes out, the trigger counting module returns to zero, and the detection ends.
[0017] A further optimization scheme is that one or more detection programs are provided on the control main board, each detection program is preset with the running status of the motor, the first timing duration and the second timing duration, and the detection programs include a chlorpyrifos detection program, an aldicarb detection program, a thiophanate-methyl detection program, an avermectin detection program, a paraquat detection program and / or a quinolone detection program.
[0018] In a further optimized solution, at least two arc-shaped elastic parts are provided on the inner wall of the sample hole and along the axial direction of the sample hole, and the arc-shaped elastic parts are distributed in a ring array around the central axis of the sample hole. Alternatively, at least two fan-shaped elastic parts are provided on the inner wall of the sample hole and along the radial direction of the sample hole, and the fan-shaped elastic parts are distributed in a ring array around the central axis of the sample hole.
[0019] According to the optimized solution, the detection groove is a long strip-shaped groove structure.
[0020] According to the optimized solution, the detection table portion, the reaction table portion and the sample table portion of the operating table are in a rectangular structure arranged in a stepped shape.
[0021] A further optimization scheme, the present invention also includes a test tube box, which is a rectangular box-shaped structure with a test tube placement plate on the top and an opening on the bottom, the test tube placement plate of the test tube box has a plurality of test tube placement holes, and one side of the top of the test tube box has a rectangular space corresponding to the reaction table portion. The inverted test tube box can be placed on the side of the sample table portion on the operating table by the test tube placement plate, so that the operating table and the inverted test tube box are combined into a portable component with a rectangular structure.
[0022] According to a further optimized solution, at least two elastic fan-shaped pieces are arranged on the test tube placement hole of the test tube box, and the elastic fan-shaped pieces are distributed in a ring array around the central axis of the test tube placement hole.
[0023] As a further optimization solution, the present invention also includes a suitcase with a rectangular shell structure, and the portable component can be placed in the suitcase.
[0024] Compared with the prior art, the present invention has the following beneficial effects:
[0025] 1. The operating table of the present invention is divided into a sample table part, a reaction table part and a detection table part, and the sample holes, reaction holes and detection slots of each part are arranged one by one, so that the whole process from sample placement to reaction and then to detection is carried out in an orderly manner, avoiding confusion of different samples, improving the convenience and accuracy of operation, and reducing the possibility of human operation errors.
[0026] 2. The sample rotating assembly of the sample platform of the present invention drives the rotating tube to rotate through a motor, so that the sample in the rotating tube is fully mixed with the added pesticide residue extract, which accelerates the reaction process and improves the detection efficiency. At the same time, it also reduces the operation steps such as manual stirring, making the experimental operation more time-saving and labor-saving.
[0027] 3. The present invention controls the flashing of the signal light through the state of the timing module to provide an intuitive timing status indication; and selects a predetermined detection program according to different detection samples to ensure that the detection personnel can complete the detection of different samples efficiently, reliably and quickly.
[0028] 4. The arc-shaped elastic part or fan-shaped elastic part on the inner wall of the sample hole of the present invention can elastically position the rotating tube and the reagent tubes such as centrifuge tubes placed in the rotating tube, thereby rotating synchronously to ensure smooth and uniform rotation of the reagent tubes during detection.
[0029] 5. The timing unit installed near the detection platform of the present invention has a timing switch function and a light flashing alarm function after the timing is completed. When conducting pesticide residue detection, the detection card corresponding to each sample accurately controls the time, and the operator is reminded of the end of the reaction in time through the light alarm, avoiding the influence of the detection result due to the inaccurate time control, and further improving the accuracy of the detection.
[0030] 6. The operating table of the present invention can be combined with the test tube box to form a portable component with a rectangular structure, and combined with a suitcase with a rectangular shell structure, the entire experimental equipment is easy to carry and store. This is very practical for situations where rice pesticide residue detection needs to be carried out in different locations, such as in fields, agricultural product purchasing points, etc., where a detection platform can be quickly built and detection work can be carried out in a timely manner.
[0031] 7. The test tube placement plate of the test tube box of the present invention is provided with a test tube placement hole, and the placement hole is provided with an elastic fan-shaped piece, which can better fix the test tube and prevent the test tube from tipping over or being damaged by collision during placement and transfer. At the same time, the test tube box can be combined with the operating table after being inverted. At the same time, the inverted test tube box can also be used to place test tubes and other testing supplies, so as to increase the placement space when storing and carrying. This not only makes full use of the space of the test tube box, but also improves its practicality. BRIEF DESCRIPTION OF THE DRAWINGS
[0032] Figure 1 It is a structural schematic diagram of the present invention.
[0033] Figure 2 It is a schematic diagram of the internal structure of the sample rotation assembly of the present invention.
[0034] Figure 3 This is a top view of a sample hole of the present invention in which an arc-shaped elastic portion is provided.
[0035] Figure 4 This is a top view of a sample hole of the present invention in which a fan-shaped elastic portion is provided.
[0036] Figure 5 and Figure 6 The present invention is provided with a test tube box assembly state schematic diagram.
[0037] Figure 7 and Figure 8 It is a schematic structural diagram of the test tube box of the present invention.
[0038] Fig. 9 It is a top view of the test tube box of the present invention.
[0039] Fig.10 It is a schematic diagram of the test tube box of the present invention in use.
[0040] Fig.11 The present invention is provided with a structural schematic diagram of a suitcase.
[0041] Fig.12 It is a schematic diagram of the electrical connection of the control mainboard of the present invention. DETAILED DESCRIPTION
[0042] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention.
[0043] Example
[0044] refer to Figures 1 to 12 , an experimental device for detecting pesticide residues in rice, comprising an operating table 1.
[0045] The operating table 1 is provided with a detection table part 11, a reaction table part 12 and a sample table part 13 from front to back. The top surface of the detection table part 11 has one or more detection slots 2 arranged in sequence from left to right. The reaction table part 12 is higher than the detection table part 11, and has one or more reaction holes 3 arranged in sequence from left to right on its top surface. The sample table part 13 is higher than the reaction table part 12, and has one or more sample holes 4 arranged in sequence from left to right on its top surface. The detection slots 2, reaction holes 3 and sample holes 4 are arranged in one-to-one correspondence.
[0046] Specific reference Figure 2A sample rotating assembly is provided on the sample platform 13 corresponding to each sample hole 4. The sample rotating assembly includes a support frame 5, a motor 51, a rotating tube 52 and a rolling bearing 53. The support frame 5 is fixedly installed in the sample platform 13. The rotating tube 52 and the motor 51 are arranged in the support frame 5 from top to bottom. The end of the output shaft of the motor 51 is fixedly connected to the bottom of the rotating tube 52, and the support frame 5 and the rotating tube 52 are rotatably connected through the rolling bearing 53, and then the rotating tube 52 is driven to rotate in the support frame 5 by the motor 51, and the tube hole of the rotating tube 52 forms the sample hole 4.
[0047] A timing unit 6 is installed on the top surface of the detection platform 11 near each detection slot 2 , and the timing unit 6 has a timing switch 61 and a signal light 62 .
[0048] When the above structure is used, taking the chlorpyrifos detection kit mentioned in the background technology to test rice samples as an example, the sample hole 4 is used to place a centrifuge tube 9, the centrifuge tube 9 is built with a sample to be tested and a pesticide residue extract, the reaction hole 3 is used to place a gold-labeled micropore (not shown in the figure), and the detection slot 2 is used to place a detection card, and the existing steps are operated in sequence. The centrifuge tube 9 is placed in the sample hole 4 formed by the rotating tube 52, and the rotating tube 52 is driven by the motor 51 to rotate, so that the sample in the centrifuge tube 9 is fully mixed with the pesticide residue extract, accelerating the reaction process, while reducing manual operation, making the experimental operation more time-saving and labor-saving. In addition, the timing unit 6 can time and remind the detection of each sample, effectively ensuring the convenience of multiple sample detection. Furthermore, the sample hole 4, the reaction hole 3 and the detection slot 2 are arranged one by one to orderly perform operations on different samples, thereby avoiding sample confusion, improving the convenience and accuracy of operation, and reducing the possibility of human error.
[0049] Specific reference Fig.12 , further, the experimental equipment of this embodiment also includes a control mainboard, on which a motor control module is provided for each motor 51 of the sample rotating assembly, and a timing control module is provided for each timing unit 6;
[0050] The motor control module is used to control the running state of the motor 51;
[0051] The timing control module is used to control the timing state of the timing unit 6, and the timing control module includes a first timing module, a second timing module and a trigger counting module; the first timing module is used to time the first timing duration, the second timing module is used to time the second timing duration, and the trigger counting module is used to record the number of triggering times of the timing switch 61;
[0052] The motor 51 of the sample rotating assembly, the timing switch 61 and the signal light 62 of the timing unit 6 are electrically connected to the control mainboard respectively;
[0053] After the timing switch 61 is triggered for the first time, the first timing module starts timing, and the signal light 62 is off;
[0054] After the first timing module has finished timing, the signal light 62 lights up and is in a flashing state;
[0055] After the timing switch 61 is triggered for the second time, the second timing module starts timing, and the signal light 62 goes out at the same time;
[0056] After the second timing module has finished timing, the signal light 62 lights up again and is in a flashing state;
[0057] After the timing switch 61 is triggered for the third time, the signal light 62 goes out, the trigger counting module returns to zero, and the detection ends.
[0058] In the above structure, the first timing duration corresponds to the duration of "waiting for reaction for 5 minutes" in step 2 of the background technology, which is the reaction duration of the test liquid and the reaction substrate in the gold-labeled microwell. The second timing duration corresponds to the duration of "start timing after adding the sample, and read the test result after 5-8 minutes" in step 3 of the background technology, which is the reaction duration after the test card drips the liquid.
[0059] After the drop of the liquid to be tested is added to the gold-labeled micropore, the timing switch 61 is triggered for the first time, and the first timing module starts timing. After the timing of the first timing module is completed, the reaction of the liquid to be tested and the reaction substrate in the gold-labeled micropore is completed, and the signal light 62 flashes to remind the tester to perform pipetting. Subsequently, after the tester adds the sample to the test card, the timing switch 61 is triggered for the second time, and the second timing module starts timing. At the same time, the signal light 62 goes out, marking that the test sample of this group is in a reaction state and no operation is required. After the predetermined second timing module timing is completed after adding the sample to the test card, the signal light 62 flashes again to remind the tester to check the test results. After the review is completed, the timing switch 61 is triggered for the third time, the signal light 62 goes out, and the test is completed so that the next test can be performed.
[0060] The control main board is provided with one or more detection programs, each of which is respectively preset with the running state of the motor 51, the first timing duration and the second timing duration, and the detection programs include a chlorpyrifos detection program, an aldicarb detection program, a thiophanate-methyl detection program, an abamectin detection program, a paraquat detection program and / or a quinolone detection program. Before the detection begins, the corresponding detection program is pre-selected according to the pesticide residue to be detected, and the subsequent operation only needs to trigger the motor start function of the corresponding motor control module and the timing switch 61 of the timing unit 6, without the need for the detection personnel to set the motor operation parameters and timing duration by themselves, which is very convenient, reliable and efficient.
[0061] Furthermore, the detection slot 2 is a long strip-shaped slot structure, and is thus compatible with a rectangular plate-shaped detection card (not shown in the figure), which is beneficial for positioning and placing the detection card.
[0062] Specific reference Figure 2 and Figure 3 Furthermore, at least two arc-shaped elastic parts 41 are provided on the inner wall of the sample hole 4 and along the axial direction of the sample hole 4, and the arc-shaped elastic parts 41 are distributed in a ring array around the central axis of the sample hole 4. In the above structure, the centrifuge tube 9 is elastically clamped by the arc-shaped elastic parts 41, so that the centrifuge tube 9 and the rotating tube 52 rotate synchronously, ensuring that the centrifuge tube 9 rotates smoothly and evenly during detection.
[0063] Or, specific reference Figure 4 The arc-shaped elastic portion 41 is replaced by a fan-shaped elastic portion 42. Specifically, at least two fan-shaped elastic portions 42 are provided on the inner wall of the sample hole 4 and along the radial direction of the sample hole 4. The fan-shaped elastic portions 42 are distributed in a circular array around the central axis of the sample hole 4. The fan-shaped elastic portions 42 also play the role of elastically clamping the centrifuge tube 9.
[0064] The detection platform 11, the reaction platform 12 and the sample platform 13 of the operating platform 1 are arranged in a stepped rectangular structure. Such a setting at different heights is conducive to the pipetting operation. In addition, a control panel 91 for controlling the running state of the motor can be set on the front of the sample platform 13. The control panel 91 is provided with operation keys corresponding to the control functions of the control mainboard and the selection functions of the detection program, which is more convenient for operation.
[0065] Specific reference Figures 5 to 10 The experimental equipment of this embodiment also includes a test tube box 7, which is a rectangular box-shaped structure with a test tube placement plate on the top and an opening on the bottom. The test tube placement plate of the test tube box 7 has a plurality of test tube placement holes 71, and one side of the top of the test tube box 7 has a rectangular space corresponding to the reaction table 12. The inverted test tube box 7 can be placed on the side of the sample table 13 on the operating table 1 by the test tube placement plate, so that the operating table 1 and the inverted test tube box 7 are combined into a portable component with a rectangular structure. Further, when combined into a portable component, specific reference is made to Fig.11 , and can also be placed in a suitcase 73 with a rectangular shell structure. In this way, the test tube box 7 can be used to place the centrifuge tube 9, and the test tube box 7 can be combined with the operating table 1 after being inverted, which can reduce the space and use the test tube box 7 as a storage box for storing test supplies. It is very suitable for on-site rapid testing in environments outside the laboratory, such as market supervision, agricultural planting, farmers' markets, etc.
[0066] Specific reference Figures 8 to 10Further, at least two elastic fan-shaped pieces 72 are provided on the test tube placement hole 71 of the test tube box 7, and the elastic fan-shaped pieces 72 are distributed in a circular array around the central axis of the test tube placement hole 71. The elastic fan-shaped pieces 72 play the role of elastically clamping the centrifuge tube 9, so that the centrifuge tube 9 can be stably inserted into the test tube placement hole 71.
Claims
1. An experimental device for detecting pesticide residues in rice, characterized in that: The operating table (1) comprises a detection table portion (11), a reaction table portion (12) and a sample table portion (13) arranged in sequence from front to back; the detection table portion (11) has one or more detection slots (2) arranged in sequence from left to right on the top surface; the reaction table portion (12) is higher than the detection table portion (11) and has one or more reaction wells (3) arranged in sequence from left to right on the top surface; the sample table portion (13) is higher than the reaction table portion (12) and has one or more sample wells (4) arranged in sequence from left to right on the top surface; the detection slots (2), reaction wells (3) and sample wells (4) are arranged in one-to-one correspondence; A sample rotating assembly is provided on the sample platform (13) corresponding to each sample hole (4), and the sample rotating assembly comprises a support frame (5), a motor (51), a rotating tube (52) and a rolling bearing (53). The support frame (5) is fixedly installed in the sample platform (13), and the rotating tube (52) and the motor (51) are arranged in the support frame (5) from top to bottom. The end of the output shaft of the motor (51) is fixedly connected to the bottom of the rotating tube (52), and the support frame (5) and the rotating tube (52) are rotatably connected via the rolling bearing (53), and then the rotating tube (52) is driven to rotate in the support frame (5) by the motor (51), and the tube hole of the rotating tube (52) forms the sample hole (4); A timing unit (6) is installed on the top surface of the detection platform (11) near each detection slot (2), and the timing unit (6) has a timing switch (61) and a signal light (62).
2. The experimental equipment for detecting pesticide residues in rice according to claim 1, characterized in that: It also includes a control main board, on which a motor control module is provided for each motor (51) of each sample rotating assembly, and a timing control module is provided for each timing unit (6); The motor control module is used to control the running state of the motor (51); The timing control module is used to control the timing state of the timing unit (6), and the timing control module includes a first timing module, a second timing module and a trigger counting module; the first timing module is used to time a first timing duration, the second timing module is used to time a second timing duration, and the trigger counting module is used to record the number of times the timing switch (61) is triggered; The motor (51) of the sample rotating assembly, the timing switch (61) and the signal light (62) of the timing unit (6) are electrically connected to the control main board respectively; After the timing switch (61) is triggered for the first time, the first timing module starts timing and the signal light (62) does not light up; After the first timing module completes the timing, the signal light (62) lights up and is in a flashing state; After the timing switch (61) is triggered for the second time, the second timing module starts timing and the signal light (62) turns off at the same time; After the second timing module has finished timing, the signal light (62) lights up again and is in a flashing state; After the timing switch (61) is triggered for the third time, the signal light (62) goes out, the trigger counting module is reset to zero, and the detection ends.
3. The experimental equipment for detecting pesticide residues in rice according to claim 2, characterized in that: The control main board is provided with one or more detection programs, each detection program is respectively preset with the running state of the motor (51), a first timing duration and a second timing duration, and the detection programs include a chlorpyrifos detection program, an aldicarb detection program, a thiophanate-methyl detection program, an avermectin detection program, a paraquat detection program and / or a quinolone detection program.
4. The experimental equipment for detecting pesticide residues in rice according to claim 1, 2 or 3, characterized in that: At least two arc-shaped elastic parts (41) are provided on the inner wall of the sample hole (4) and along the axial direction of the sample hole (4), and the arc-shaped elastic parts (41) are distributed in a ring array around the central axis of the sample hole (4).
5. The experimental equipment for detecting pesticide residues in rice according to claim 1, 2 or 3, characterized in that: At least two fan-shaped elastic parts (42) are arranged on the inner wall of the sample hole (4) and along the radial direction of the sample hole (4), and the fan-shaped elastic parts (42) are distributed in a ring array around the central axis of the sample hole (4).
6. The experimental equipment for detecting pesticide residues in rice according to claim 1, 2 or 3, characterized in that: The detection slot (2) is in the form of a long strip of slot-like structure.
7. The experimental equipment for detecting pesticide residues in rice according to claim 1, 2 or 3, characterized in that: The detection platform part (11), the reaction platform part (12) and the sample platform part (13) of the operating platform (1) are rectangular structures arranged in a stepped shape.
8. The experimental equipment for detecting pesticide residues in rice according to claim 7, characterized in that: The invention also comprises a test tube box (7), wherein the test tube box (7) is a rectangular box-shaped structure having a test tube placement plate on the top and an opening on the bottom, wherein the test tube placement plate of the test tube box (7) has a plurality of test tube placement holes (71), and one side of the top of the test tube box (7) has a rectangular space corresponding to the reaction table (12). The inverted test tube box (7) can be placed on the operating table (1) on one side of the sample table (13) by the test tube placement plate, so that the operating table (1) and the inverted test tube box (7) are combined into a portable component with a rectangular structure.
9. The experimental equipment for detecting pesticide residues in rice according to claim 8, characterized in that: At least two elastic fan-shaped pieces (72) are arranged on the test tube placement hole (71) of the test tube box (7), and the elastic fan-shaped pieces (72) are distributed in a ring array around the central axis of the test tube placement hole (71).
10. The experimental equipment for detecting pesticide residues in rice according to claim 8 or 9, characterized in that: Also included is a suitcase (73) having a rectangular shell structure, wherein the portable assembly can be placed in the suitcase (73).