Device for simulating perspiration of human epidermis and manufacturing method thereof

Abstract

The invention belongs to the technical field of micro-nano manufacturing and sensors, and particularly discloses a device for simulating perspiration of human epidermis and a manufacturing method thereof, the device comprises a perspiration module, an injector and an injection pump; the injector is installed on the injection pump, liquid in the injector is pushed by a movement mechanism on the injection pump to be injected into the perspiration module, and the injector is connected with the perspiration module through a hose; the perspiration module is composed of an artificial epidermal layer, a porous membrane layer, a first sealing gasket, a supporting frame, a second sealing gasket, a lower cavity, a liquid guide pipe, an upper cover plate and microfibers. According to the device for simulating perspiration of the human skin and the manufacturing method of the device, the device has the advantages of being uniform in perspiration, good in controllability, high in control precision and the like; according to the manufacturing method, a real human body skin reverse mold processing mode is adopted, so that the surface appearance and mechanical characteristics of the human body skin are closer to those of real skin, and the simulation precision of human body perspiration behaviors is higher.

Description

Technical Field

[0001] The invention belongs to the field of micro-nano manufacturing and sensor technology, and specifically relates to a device for simulating human epidermal perspiration and a manufacturing method thereof. Background Art

[0002] In recent years, wearable sensing technology has attracted more and more attention and interest. Wearable sensors for measuring body parameters such as electrocardiogram and electromechanical are widely used among consumers. In addition to these physical signals, wearable solutions are also used to measure biomarkers in human biofluids (such as tears, sweat and saliva) and detect biological and chemical signals of the human body for use by patients with chronic diseases, practitioners in special industries and ordinary consumers. Among them, sweat is an ideal research object for long-term, semi-continuous and non-aggressive health monitoring because it is a continuously available biofluid that contains rich physiological and metabolic information. It is easier than blood, tears and saliva.

[0003] In the process of developing wearable sweat sensors, it is necessary to test their collection, manipulation and flow in the presence of sweat. Currently, this part of the work requires expensive human subject testing, which limits the development cycle and testing time of wearable sweat sensors. Especially in the early development stage, in vivo testing on humans is not appropriate because there is a large variability in skin and sweat characteristics between people and even within a single person. In addition, sweat characteristics cannot be well controlled in in vivo testing. Therefore, it is crucial to develop a suitable human epidermal sweat test platform to test the performance of wearable sensors.

[0004] Existing research has explored devices that simulate human epidermal perspiration: Chinese invention patent 201911334647.7 discloses an artificial epidermal structure that seeks to expand the range of changes in the friction force acting on the object and adjust its surface friction performance, but has nothing to do with the perspiration process. Chinese invention patent 202010227486.8 discloses a method for preparing an artificial epidermal cosmetics testing kit and its application. Human skin tissue cells: human fibroblasts and epidermal cells are cultured on a scaffold material to prepare an artificial epidermis with a dermis and epidermis structure. The prepared artificial epidermal cosmetics testing kit can detect the irritation, corrosion, anti-aging, antioxidant, whitening effect of cosmetics on human skin, etc., rather than for perspiration testing. Scholars from Nanjing University of Science and Technology proposed the design principle and preparation method of an artificial microfluidic "skin" layer based on a double-layer membrane structure design. The upper layer of the artificial microfluidic "skin" layer uses rigid materials such as stainless steel to maintain its structural stability. This artificial microfluidic "skin" can be used for wearable sweat sensor testing, but using metal materials to simulate human skin cannot fully simulate the effects of human skin surface texture and sweat hair on fluid flow.

[0005] At present, the difficulties in developing a device that simulates human epidermal perspiration lie in the following aspects. First, the surface of human skin has certain grooves and lines, which are difficult to design or manufacture directly; second, the sweat hairs in the human sweat pores have an important influence on the discharge and flow of sweat, and existing studies have not considered this issue; finally, due to the limitations of manufacturing technology, the size consistency of the processed micro-sweat pores is poor. During the perspiration test, uneven sweat flow often occurs, which is difficult to overcome, and it is very difficult to manufacture a device that simulates human epidermal perspiration.

[0006] Therefore, there is a need in the art to develop a device for simulating human epidermal perspiration and a manufacturing method thereof, which can effectively solve the above problems. Summary of the invention

[0007] The purpose of the present invention is to provide a device for simulating human epidermal perspiration and a manufacturing method thereof, wherein the device has the advantages of uniform perspiration, good controllability, and high control accuracy; the manufacturing method adopts a real human skin mold processing method to make its surface morphology and mechanical properties closer to real skin, and the simulation accuracy of human perspiration behavior is higher.

[0008] To achieve the above-mentioned purpose, the present invention provides a device for simulating human epidermal perspiration, comprising a perspiration module, a syringe, and an injection pump; the syringe is installed on the injection pump, and the motion mechanism on the injection pump pushes the liquid in the syringe to be injected into the perspiration module, and the syringe and the perspiration module are connected by a hose; the perspiration module is composed of an artificial epidermis layer, a porous membrane layer, a first sealing pad, a support frame, a second sealing pad, a lower cavity, a liquid guide tube, an upper cover plate and microfibers.

[0009] Preferably, the upper surface of the porous membrane layer is in contact with the lower surface of the artificial skin layer, the first sealing gasket is installed on the lower surface of the porous membrane layer, the support frame is installed on the lower surface of the first sealing gasket, and the second sealing gasket is installed on the lower surface of the support frame;

[0010] The liquid in the syringe and the hose is introduced into the lower cavity through the catheter; the volume specification of the syringe is 2mL, and the delivery range of the injection pump is 10mL / min-13mL / min.

[0011] Preferably, the artificial epidermis layer is made of one of human silicone, silicone rubber and siloxane after curing, and its surface has the same grooves and patterns as human skin; a plurality of micro-through holes are distributed on the artificial epidermis layer to form a group hole structure.

[0012] Preferably, the density of micro-through holes is 60-400 / cm 2The micro-through holes are evenly distributed on the artificial epidermis layer; the size of the micro-through holes is close to that of human sweat pores, which is 80-150 μm; the porous membrane layer is provided with pores, and the size of the pores is 0.01-30 μm, which is smaller than the pore size of the micro-through holes on the artificial epidermis layer.

[0013] Preferably, the porous membrane layer, the first sealing pad, the support frame and the second sealing pad are integrally located inside the lower cavity; the lower cavity and the upper cover plate are installed together by means of threads or screws to compress the artificial epidermis layer, the porous membrane layer, the first sealing pad, the support frame and the second sealing pad inside.

[0014] Preferably, the microfiber simulates human sweat hair, and the diameter of the microfiber is 10-30 μm; it is located inside the micro-through hole of the artificial epidermis layer, one end of which protrudes 3-5 mm from the outer surface of the artificial epidermis layer, and the other end of which is inserted into and fixed in the pores of the porous membrane layer; the material of the microfiber is acrylic.

[0015] A method for manufacturing a device for simulating human epidermal perspiration, comprising the following steps:

[0016] Step S1, taking human skin as the object, performing inverted molding to make an artificial epidermis mold;

[0017] Step S2, installing the artificial epidermis mold in the lower mold cavity, processing a group hole structure on the artificial epidermis mold and the lower mold cavity; the group hole structure on the artificial epidermis mold serves as sweat pores;

[0018] Step S3, installing the upper mold cavity with the hole group structure corresponding to the lower mold cavity on the lower mold cavity; fixing the upper mold cavity and the lower mold cavity, and defining the required thickness of the artificial epidermis layer;

[0019] Step S4, passing the metal wire through the upper mold cavity, the lower mold cavity and the artificial epidermis mold;

[0020] The diameter of the metal wire is 80-150 μm, and the metal wire material is one of stainless steel, copper, molybdenum and tungsten;

[0021] Step S5, tensioning the metal wire with a metal wire tensioning device to make it in a vertical state, then injecting one of human silicone, silicone rubber, and siloxane into the mold cavity, and placing it in an oven at 25-30° C. to cure for 10-12 hours to cast an artificial epidermis layer;

[0022] Step S6, removing the metal wire, the upper mold cavity and the lower mold cavity to form an artificial epidermis layer with micro-through holes and skin texture;

[0023] Step S7, sequentially attaching and installing a porous membrane layer, a first sealing gasket, a support frame, and a second sealing gasket on the flat side of the artificial epidermis layer; applying 100-200 μm of adhesive along the edge of each layer to ensure that the layers remain bonded and do not fall off;

[0024] Step S8, installing the artificial skin layer with the porous membrane layer, the first sealing gasket, the support frame and the second sealing gasket inside the lower cavity of the device as a whole, ensuring that the upper surface of each stack is higher than the upper surface of the lower cavity;

[0025] Step S9, installing the upper cover plate, pressing and fixing the artificial epidermis layer, the porous membrane, the first sealing pad, the support frame and the second sealing pad inside the lower cavity, so that the surface of the artificial epidermis layer is slightly deformed to ensure precise fitting between the layers; the upper cover plate and the lower cavity are connected by threads or screws;

[0026] Step S10, inserting microfibers into the micro-through holes of the artificial epidermis layer, wherein the microfibers are inserted into the interior of the artificial epidermis layer, one end of the microfibers protrudes 3-5 mm from the surface of the artificial epidermis layer, and the other end is inserted into and fixed in the pores of the porous membrane layer, thereby obtaining a perspiration module;

[0027] Step S11, installing the syringe on the syringe pump, connecting the syringe and the perspiration module through a hose, and finally forming an artificial epidermis perspiration device.

[0028] Preferably, step S1 specifically comprises:

[0029] Step S11, firstly applying a polyurethane-silicone mixture to replicate silicone on human skin, wherein the proportion of silicone in the polyurethane-silicone mixture is 10%-50%;

[0030] Step S12, curing for 14-16 minutes at room temperature and a humidity of 40%-60%; peeling off after curing to produce an artificial epidermis mold;

[0031] Step S13, spraying the artificial skin mold with a release agent and then drying it.

[0032] Preferably, in step S2, the hole group structure on the artificial epidermis mold is processed by micro-drilling; the hole group structure on the lower mold cavity is processed by micro-drilling or laser processing.

[0033] Preferably, the materials of the upper mold cavity and the lower mold cavity are both one of polymethyl methacrylate polymer materials, copper metal materials, and aluminum metal materials.

[0034] The present invention adopts the above-mentioned device for simulating human epidermal perspiration and the manufacturing method thereof, and the beneficial effects are as follows:

[0035] (1) The device of the present invention is adaptable in terms of wetting properties, skin texture, sweat pore density and fluid drive, close to human skin, so as to adapt to different skin and sweat properties and simulate specific body locations and applications of sweat sensing devices;

[0036] (2) The device of the present invention adopts a double-layer structure of an artificial epidermis layer and a porous membrane layer, and has uniform perspiration, good controllability, and high control accuracy;

[0037] (3) The device of the present invention is prepared by using a real human skin mold processing method, so that its surface morphology and mechanical properties are closer to real skin, and the simulation accuracy of human perspiration behavior is higher;

[0038] (4) The manufacturing method of the perspiration module of the device for simulating perspiration of the human epidermis in the present invention is simple in process, low in cost, and can be automated.

[0039] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 A schematic diagram of the structure of an embodiment of a device for simulating human epidermal perspiration and a method for manufacturing the same according to the present invention;

[0041] Figure 2 A flow chart of a manufacturing method of a device for simulating human epidermal perspiration and a manufacturing method thereof according to an embodiment of the present invention;

[0042] Figure 3 A schematic diagram of a human skin mold-turning process in an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof according to the present invention;

[0043] Figure 4 A schematic diagram of the processing technology of the sweating group hole structure of an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof of the present invention;

[0044] Figure 5 This is a schematic diagram of installing an artificial epidermis mold according to an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof of the present invention;

[0045] Figure 6 A schematic diagram of metal wire installation in an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof according to the present invention;

[0046] Figure 7 A schematic diagram of manufacturing an artificial epidermis layer according to an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof of the present invention;

[0047] Figure 8 This is a schematic diagram of the demoulding completion of the artificial epidermis layer of a device for simulating human epidermal perspiration and a manufacturing method thereof according to an embodiment of the present invention;

[0048] Fig. 9 This is a schematic diagram of the installation of an artificial epidermis layer and a porous membrane layer in an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof according to the present invention;

[0049] Fig.10 This is a schematic diagram of the installation of a support frame and a sealing pad in an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof according to the present invention;

[0050] Fig.11 This is a schematic diagram of the installation of a liquid guide tube and a lower cover plate in an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof according to the present invention;

[0051] Fig.12 This is a schematic diagram of the installation of the upper cover plate of an embodiment of a device for simulating human epidermal perspiration and a manufacturing method thereof according to the present invention;

[0052] Fig.13 The present invention is a schematic diagram of microfiber installation in an embodiment of a device for simulating human epidermal perspiration and a method for manufacturing the same.

[0053] Reference numerals

[0054] 1. Perspiration module; 101. Artificial epidermis layer; 102. Porous membrane layer; 103. First sealing pad; 104. Support frame; 105. Second sealing pad; 106. Lower cavity; 107. Liquid guide tube; 108. Upper cover plate; 109. Microfiber;

[0055] 2. Syringe; 3. Syringe pump; 4. Artificial epidermis mold; 5. Lower mold cavity; 6. Upper mold cavity; 7. Metal wire; 8. Tensioning device. DETAILED DESCRIPTION

[0056] The technical solution of the present invention is further described below through the accompanying drawings and embodiments.

[0057] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0058] like Figure 1 As shown, a device for simulating human epidermal perspiration includes a perspiration module 1, a syringe 2, and an injection pump 3. The syringe 2 is mounted on the injection pump 3, and the motion mechanism on the injection pump 3 drives the liquid in the syringe 2 to be injected into the perspiration module 1. The syringe 2 and the perspiration module 1 are connected by a hose. The liquid in the syringe 2 and the hose is introduced into the lower cavity 106 through the liquid guide tube 107. The volume specification of the syringe 2 is 2mL, and the delivery range of the injection pump 3 is 10mL / min-13mL / min.

[0059] The perspiration module 1 is composed of an artificial epidermis layer 101 , a porous membrane layer 102 , a first sealing pad 103 , a support frame 104 , a second sealing pad 105 , a lower cavity 106 , a liquid guide tube 107 , an upper cover plate 108 and microfibers 109 .

[0060] The upper surface of the porous film layer 102 is fitted with the lower surface of the artificial epidermis layer 101, and the effect of uniform sweating under low flow rate can be achieved. The first sealing pad 103 is installed on the lower surface of the porous film layer 102 to play a liquid sealing role to prevent leakage. The support frame 104 is installed on the lower surface of the first sealing pad 103 to support the aforementioned artificial epidermis layer 101, the porous film layer 102 and the first sealing pad 103 to ensure that the fluid flows smoothly through the porous film layer 102 and the artificial epidermis layer 101 to prevent the deformation of the two under pressure from being too large. The second sealing pad 105 is installed on the lower surface of the support frame 104 to play a liquid sealing role to prevent leakage.

[0061] The artificial epidermis layer 101 is made of one of human silicone, silicone rubber, and siloxane after curing, and its surface has the same grooves and lines as human skin. There are a number of micro-through holes distributed on the artificial epidermis layer 101, forming a group hole structure. According to the density of sweat glands in different parts of the human body, the density of micro-through holes is 60-400 / cm 2 The micro-through holes are evenly distributed on the artificial epidermis layer 101. The size of the micro-through holes is close to that of the sweat pores of the human body, which is 80-150 μm.

[0062] The porous membrane layer 102 is provided with pores, and the size of the pores is 0.01-30 μm, which is much smaller than the diameter of the micro-through holes on the artificial epidermis layer 101 .

[0063] The porous membrane layer 102, the first sealing gasket 103, the support frame 104 and the second sealing gasket 105 are integrally located inside the lower cavity 106. The lower cavity 106 and the upper cover plate 108 are assembled together by threads or screws, and the artificial epidermis layer 101, the porous membrane layer 102, the first sealing gasket 103, the support frame 104 and the second sealing gasket 105 inside are pressed tightly to prevent leakage.

[0064] The microfiber 109 simulates human hair and has a diameter of 10-30 μm. It is located inside the micro-through hole of the artificial epidermis layer 101, with one end extending 3-5 mm from the outer surface of the artificial epidermis layer 101 and the other end inserted and fixed in the pores of the porous membrane layer 102. The material of the microfiber 109 is acrylic.

[0065] Example

[0066] like Figure 2-Figure 13 As shown, a method for manufacturing a device for simulating human epidermal perspiration comprises the following steps:

[0067] Step S1, taking human skin as the object, performing mold casting to make an artificial epidermis mold 4.

[0068] Step S11, firstly, applying a polyurethane-silicone mixture to replicate silicone on human skin, wherein the proportion of silicone in the polyurethane-silicone mixture is 30%.

[0069] Step S12, curing for 15 minutes at room temperature and 50% humidity, and peeling off after curing to produce an artificial epidermis mold 4.

[0070] Step S13: spray the artificial skin mold 4 with a release agent (Mann Ease release 2831) and then dry it.

[0071] Step S2, installing the artificial epidermis mold 4 in the lower mold cavity 5, the material of the lower mold cavity 5 is aluminum metal material. Processing a group hole structure on the artificial epidermis mold 4 and the lower mold cavity 5. The group hole structure on the artificial epidermis mold 4 is used as sweat pores.

[0072] The hole group structures on the lower mold cavity 5 and the artificial epidermis mold 4 are processed by micro-drilling, and the aperture of the micro-through hole is 100 μm.

[0073] Step S3, installing the upper mold cavity 6 with the hole group structure corresponding to the lower mold cavity 5 on the lower mold cavity 5, the upper mold cavity 6 is made of aluminum metal material, fixing the upper mold cavity 6 and the lower mold cavity 5, and defining the required thickness of the artificial skin layer 101 to be 6 mm.

[0074] Step S4 , passing a tungsten wire with a diameter of 100 μm through the upper mold cavity 6 , the lower mold cavity 5 and the artificial epidermis mold 4 .

[0075] Step S5, use the metal wire 7 tensioning device 8 to tension the tungsten wire to make it in a vertical state, then inject silicone (Resion silicone rubber shore 15) into the mold cavity, and place it in an oven at 25° C. to cure for 12 hours to cast the artificial epidermis layer 101.

[0076] Step S6, remove the tungsten wire, the upper mold cavity 6 and the lower mold cavity 5, remove the burrs, flash and residues on the surface, and form an artificial epidermis layer 101 with micro-through holes and skin texture. The artificial epidermis layer 101 is distributed with 400 evenly arranged micro-holes with a pore size of 100 μm.

[0077] Step S7: The porous membrane layer 102, the first sealing gasket 103, the support frame 104 and the second sealing gasket 105 are sequentially attached to the flat side of the artificial epidermis layer 101, wherein the pore size of the porous membrane layer 102 is 10 μm. At the edge of each layer, a 150 μm adhesive is applied along the edge to ensure that the layers remain bonded and do not fall off.

[0078] Step S8, install the artificial epidermis layer 101 with the porous membrane layer 102, the first sealing gasket 103, the support frame 104 and the second sealing gasket 105 inside the lower cavity 106 of the device as a whole, ensuring that the upper surface of each stack is higher than the upper surface of the lower cavity 106.

[0079] Step S9, install the upper cover plate 108, press and fix the artificial epidermis layer 101, the porous membrane, the first sealing gasket 103, the support frame 104 and the second sealing gasket 105 inside the lower cavity 106, so that the surface of the artificial epidermis layer 101 is slightly deformed to ensure the precise fit between the layers. The upper cover plate 108 and the lower cavity 106 are connected by threads.

[0080] Step S10, inserting a microfiber 109 with a diameter of 10 μm into the micro-through holes of the artificial epidermis layer 101, the microfiber 109 is inserted into the interior of the artificial epidermis layer 101, one end of the microfiber 109 protrudes 3 mm from the surface of the artificial epidermis layer 101, and the other end is inserted into and fixed in the pores of the porous membrane layer 102 to obtain a perspiration module 1, the size of the perspiration module 1 is 2 cm×2 cm.

[0081] Step S11, install the syringe 2 with a volume specification of 2 mL on the syringe pump 3, and the delivery volume of the syringe pump 3 is 12 mL / min. The syringe 2 and the perspiration module 1 are connected by a hose, and finally an artificial epidermis perspiration device is formed.

[0082] Therefore, the present invention adopts the above-mentioned device for simulating human epidermal perspiration and its manufacturing method. The device has the advantages of uniform perspiration, good controllability, and high control accuracy; the manufacturing method adopts the method of reverse molding of real human skin to make its surface morphology and mechanical properties closer to real skin, and the simulation accuracy of human perspiration behavior is higher.

[0083] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A device for simulating perspiration of human epidermis, characterized in that: It includes a sweating module, a syringe, and an injection pump; the syringe is installed on the injection pump, and the motion mechanism on the injection pump pushes the liquid in the syringe to be injected into the sweating module, and the syringe and the sweating module are connected by a hose; the sweating module is composed of an artificial epidermis layer, a porous membrane layer, a first sealing pad, a support frame, a second sealing pad, a lower cavity, a liquid guide tube, an upper cover plate and microfibers.

2. The device for simulating human epidermal perspiration according to claim 1, characterized in that: The upper surface of the porous membrane layer is attached to the lower surface of the artificial skin layer, the first sealing gasket is installed on the lower surface of the porous membrane layer, the support frame is installed on the lower surface of the first sealing gasket, and the second sealing gasket is installed on the lower surface of the support frame; The liquid in the syringe and the hose is introduced into the lower cavity through the catheter; the volume specification of the syringe is 2mL, and the delivery range of the injection pump is 10mL / min-13mL / min.

3. The device for simulating human epidermal perspiration according to claim 2, characterized in that: The artificial epidermis layer is made of one of human silicone, silicone rubber and siloxane after curing, and its surface has the same grooves and patterns as human skin; a number of micro-through holes are distributed on the artificial epidermis layer, forming a group pore structure.

4. The device for simulating human epidermal perspiration according to claim 3, characterized in that: The density of micro-through holes is 60-400 / cm 2 The micro-through holes are evenly distributed on the artificial epidermis layer; the size of the micro-through holes is close to that of human sweat pores, which is 80-150 μm; the porous membrane layer is provided with pores, and the size of the pores is 0.01-30 μm, which is smaller than the pore size of the micro-through holes on the artificial epidermis layer.

5. The device for simulating human epidermal perspiration according to claim 4, characterized in that: The porous membrane layer, the first sealing pad, the support frame and the second sealing pad are integrally located inside the lower cavity; the lower cavity and the upper cover plate are assembled together by threads or screws to compress the artificial epidermis layer, the porous membrane layer, the first sealing pad, the support frame and the second sealing pad inside.

6. The device for simulating human epidermal perspiration according to claim 5, characterized in that: The microfiber simulates human sweat hair, and the diameter of the microfiber is 10-30μm; it is located inside the micro-through hole of the artificial epidermis layer, one end of which protrudes 3-5mm from the outer surface of the artificial epidermis layer, and the other end of which is inserted into and fixed in the pores of the porous membrane layer; the material of the microfiber is acrylic.

7. A method for manufacturing a device for simulating human epidermal perspiration as claimed in claim 6, characterized in that: The following steps are involved: Step S1, taking human skin as the object, performing inverted molding to make an artificial epidermis mold; Step S2, installing the artificial epidermis mold in the lower mold cavity, processing a group hole structure on the artificial epidermis mold and the lower mold cavity; the group hole structure on the artificial epidermis mold serves as sweat pores; Step S3, installing the upper mold cavity with the hole group structure corresponding to the lower mold cavity on the lower mold cavity; fixing the upper mold cavity and the lower mold cavity, and defining the required thickness of the artificial epidermis layer; Step S4, passing the metal wire through the upper mold cavity, the lower mold cavity and the artificial epidermis mold; The diameter of the metal wire is 80-150 μm, and the metal wire material is one of stainless steel, copper, molybdenum and tungsten; Step S5, tensioning the metal wire with a metal wire tensioning device to make it in a vertical state, then injecting one of human silicone, silicone rubber, and siloxane into the mold cavity, and placing it in an oven at 25-30° C. to cure for 10-12 hours to cast an artificial epidermis layer; Step S6, removing the metal wire, the upper mold cavity and the lower mold cavity to form an artificial epidermis layer with micro-through holes and skin texture; Step S7, sequentially attaching and installing a porous membrane layer, a first sealing gasket, a support frame, and a second sealing gasket on the flat side of the artificial epidermis layer; applying 100-200 μm of adhesive along the edge of each layer to ensure that the layers remain bonded and do not fall off; Step S8, installing the artificial skin layer with the porous membrane layer, the first sealing gasket, the support frame and the second sealing gasket inside the lower cavity of the device as a whole, ensuring that the upper surface of each stack is higher than the upper surface of the lower cavity; Step S9, installing the upper cover plate, pressing and fixing the artificial epidermis layer, the porous membrane, the first sealing pad, the support frame and the second sealing pad inside the lower cavity, so that the surface of the artificial epidermis layer is slightly deformed to ensure precise fitting between the layers; the upper cover plate and the lower cavity are connected by threads or screws; Step S10, inserting microfibers into the micro-through holes of the artificial epidermis layer, wherein the microfibers are inserted into the interior of the artificial epidermis layer, one end of the microfibers protrudes 3-5 mm from the surface of the artificial epidermis layer, and the other end is inserted into and fixed in the pores of the porous membrane layer, thereby obtaining a perspiration module; Step S11, installing the syringe on the syringe pump, connecting the syringe and the perspiration module through a hose, and finally forming an artificial epidermis perspiration device.

8. The method for manufacturing a device for simulating human epidermal perspiration according to claim 7, characterized in that: Step S1 specifically comprises: Step S11, firstly applying a polyurethane-silicone mixture to replicate silicone on human skin, wherein the proportion of silicone in the polyurethane-silicone mixture is 10%-50%; Step S12, curing for 14-16 minutes at room temperature and a humidity of 40%-60%; peeling off after curing to produce an artificial epidermis mold; Step S13, spraying the artificial skin mold with a release agent and then drying it.

9. The method for manufacturing a device for simulating human epidermal perspiration according to claim 7, characterized in that: In step S2, the hole group structure on the artificial epidermis mold is processed by micro-drilling; the hole group structure on the lower mold cavity is processed by micro-drilling or laser processing.

10. The method for manufacturing a device for simulating human epidermal perspiration according to claim 7, characterized in that: The materials of the upper mold cavity and the lower mold cavity are both one of polymethyl methacrylate polymer materials, copper metal materials and aluminum metal materials.

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