Cosmetic formulations comprising stable, homogeneous aqueous suspensions of biopolymers

Abstract

Described herein are cosmetic compositions comprising biopolymer molecules, preferably chitosan, that have been mechanically processed into a stable, homogeneous aqueous suspension having insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent. The cosmetic composition may be formulated as a sunscreen composition, a skin care composition, or a hair care composition. In some embodiments, the biopolymer is selected from the group consisting of chitin, chitosan, cellulose, hemicellulose, lignin, amylose, actin, fibrin, collagen, silk, fibroin, keratin, wool, alginic acid, and mixtures thereof. In further embodiments, the biopolymer has been mechanically processed under high shear conditions and / or at high mechanical energy to obtain a paste, ointment, cream, lotion, gel or emulsion. In a further embodiment, the biopolymer is used to replace the microplastic in a cosmetic composition, where the cosmetic composition may be a sunscreen composition, a skin care composition, or a hair care composition.

Description

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims priority to provisional patent application US 63 / 356,665 (filed on June 29, 2022), the contents of which are incorporated herein in their entirety. Technical Field

[0003] The present invention relates to the field of cosmetics, more particularly to novel formulations for sun protection, skin care and / or hair care, comprising a homogeneous biopolymer suspension. Background Art

[0004] The beauty industry is always looking for new ingredients that are natural, biocompatible, biodegradable and non-toxic. For example, microplastics are commonly used in the beauty industry, but there are not many environmentally friendly alternatives.

[0005] Natural polymers or biopolymers are abundant natural and renewable polymers, making them an attractive resource for use in the beauty industry. However, the most abundant biopolymers such as cellulose and chitin are insoluble, which limits and complicates their use. Advantageously, the applicant describes in WO 2022 / 137184 a new homogeneous biopolymer suspension that is homogeneous and stable and can have many applications in the beauty industry.

[0006] Therefore, there is still a need for a biodegradable alternative to replace microplastics in beauty products.

[0007] Furthermore, there remains a need for cosmetic formulations, in particular sunscreen, skin care and hair care formulations, comprising natural, biocompatible, biodegradable and non-toxic ingredients such as cellulose, chitin, etc.

[0008] The present invention addresses these needs and other needs, which will become apparent from a review of the following disclosure and description of the features of the invention. Summary of the invention

[0009] According to one aspect, the present invention relates to a cosmetic composition comprising biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension, wherein the stable homogeneous aqueous suspension comprises insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent.

[0010] According to another aspect, the present invention relates to a sunscreen composition comprising: a biopolymer and at least one of a sun protection factor (SPF) booster, a sensory enhancer, a film former, an emulsifier, a co-emulsifier, an emollient, a UVB filter, a UVA filter and a broad spectrum filter.

[0011] According to another aspect, the present invention relates to the use of a sunscreen composition as defined herein for protecting the skin from the sun.

[0012] According to another aspect, the present invention relates to a method of skin protection comprising applying to the skin a sunscreen composition as defined herein.

[0013] According to another aspect, the present invention is directed to a skin care composition comprising: a biopolymer, and at least one of a moisturizer, an emollient, an occlusive agent, a ceramide, an emulsifier, a thickener, a preservative, an antioxidant, a fragrance, and a functional ingredient.

[0014] According to another aspect, the present invention relates to the use of a skin care composition as defined herein for benefiting the skin, such as moisturizing the skin, improving skin appearance, protecting from environmental damage, combating the effects of aging, removing wrinkles and / or sun spots, etc.

[0015] According to another aspect, the present invention relates to a method of protecting the skin and / or improving the appearance of the skin, which comprises applying to the skin a skin care composition as defined herein.

[0016] According to another aspect, the present invention is directed to a hair care composition comprising: a biopolymer and at least one of a surfactant, a conditioning surfactant, a co-surfactant, a conditioning softener, a thickener, an opacifier, a ceramide, a conditioning agent, a pH buffer, a chelating agent, a preservative, an emulsifier, a stabilizer, an antioxidant, a fragrance, and a functional ingredient.

[0017] According to another aspect, the present invention relates to the use of biopolymers for replacing microplastics in cosmetic compositions, said use comprising replacing at least a portion of said microplastics with a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent.

[0018] According to another aspect, the present invention is directed to a method for replacing microplastics in a sunscreen composition, comprising: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; and (2) mixing the biopolymer suspension with at least one of a sensory enhancer, a film former, an emulsifier, an emollient, a UVB filter, a UVA filter, and a broad-spectrum filter.

[0019] According to another aspect, the present invention is directed to a method for replacing microplastics in a skin care composition, comprising: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; and (2) mixing the biopolymer suspension with at least one of a moisturizer, an emollient, an occlusive agent, a ceramide, an emulsifier, a thickener, a preservative, an antioxidant, a fragrance, and a functional ingredient.

[0020] According to another aspect, the present invention is directed to a method for replacing microplastics in a hair care composition, comprising: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; and (2) mixing the biopolymer suspension with at least one of a surfactant, a conditioning surfactant, a co-surfactant, a conditioning softener, a thickener, an opacifier, a ceramide, a conditioning agent, a pH buffer, a chelating agent, a preservative, an emulsifier, a stabilizer, an antioxidant, a fragrance, and a functional ingredient.

[0021] Other aspects, advantages and features of the present invention will become more apparent upon reading the following non-limiting description of preferred embodiments, which are exemplary and should not be construed as limiting the scope of the invention. DETAILED DESCRIPTION

[0022] In the following description of the embodiments, examples in which the present invention can be practiced are described. It should be understood that other embodiments may be formed without departing from the scope of the disclosed invention. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention belongs.

[0023] General Overview

[0024] In PCT publication WO 2022 / 137184, entitled “HOMOGENEOUSBIOPOLYMER SUSPENSIONS, PROCESSES FOR MAKING SAME AND USES THEREOF,” the contents of which are incorporated herein by reference in their entirety, applicants describe the preparation of stable, homogeneous suspensions of insoluble and / or semi-soluble biopolymers.

[0025] The present invention generally relates to the use of such homogeneous biopolymer suspensions in the manufacture of "cosmetic compositions" or "cosmetic preparations" (the two terms are used interchangeably herein). Specific aspects relate to novel sunscreen, skin care and hair care preparations / compositions and methods for obtaining the same. The present invention includes the general compositions and specific compositions described in WO 2022 / 137184, but is not limited thereto.

[0026] Biopolymers

[0027] Biopolymers are minimally required according to the cosmetic composition of the present invention (e.g., sunscreen, skin care and / or hair care compositions). As used herein, the term "biopolymer" refers to a natural polymer produced by the cells of a living organism. Biopolymers are composed of monomer units that are covalently bonded to form larger molecules. The present invention includes polypeptides, polysaccharides, and polynucleotide biopolymers. Other examples of biopolymers include natural rubber (polymers of isoprene), suberin and lignin (composite polyphenol polymers), cutin and cutan (composite polymers of long-chain fatty acids), and melanin. In an embodiment, the biopolymers used as starting materials and obtained in a suspension mode are substantially pure, i.e., they are only composed of purified natural polymers.

[0028] Preferably, the biopolymer used in the cosmetic formulation is substantially free of chemical residues, and any such chemical residues are absent or present in undetectable amounts or trace amounts. Preferably, the biopolymer is substantially free of chemical residues, and any such chemical residues are absent or present in undetectable amounts or trace amounts. As used herein, "substantially free of chemical residues" means such as acids, bases, reactive chemicals, organic and / or inorganic salts, surfactants, dispersants (such as Twin 80 TM ), silanizing agents, acrylamide, etc. are completely absent or only present in undetectable or trace amounts in the final composition or final suspension. In embodiments, the biopolymer may constitute at least 98 wt % or at least 99 wt % or at least 99.9 wt % or at least 99.99 wt % of the organic compounds in the biopolymer composition or suspension, i.e., the biopolymer composition or suspension may contain less than 2 wt % or less than 1 wt %, less than 0.1 wt % or less than 0.01 wt % or less than 0.001 wt % of organic components other than the biopolymer or degradation products.

[0029] According to the present invention, biopolymers can be insoluble in water or semi-soluble in water. As used herein, the term "insoluble biopolymer" refers to a biopolymer that is "insoluble" in a polar solvent (particularly water), and the term includes equivalent terms such as "water-insoluble", "water-insoluble", "water-insoluble" or "insoluble". Insolubility can usually be observed by separation, i.e., there are two separated phases in an aqueous mixture, such as biopolymers depositing / precipitating at the bottom of an aqueous mixture or floating on the top of an aqueous mixture. According to the present invention, examples of insoluble biopolymers include chitin, chitosan, cellulose, hemicellulose, lignin, amylose, actin, fibrin, collagen, silk, silk protein, keratin, wool, alginic acid and mixtures thereof, but are not limited thereto. As used herein, the term "semi-soluble (semi-soluble) biopolymer" refers to a biopolymer that can be dissolved in a polar solvent (e.g., water) only under certain conditions (e.g., molecular weight, heat, added chemicals (e.g., acid, alcohol, surfactant, etc.)). According to the present invention, examples of semi-soluble biopolymers include gelatin, pectin, starch, pullulan, agarose, hyaluronic acid, RNA, DNA, xanthan gum, latex, polymannan, suberin, keratin, horny carbon and mixtures thereof, but are not limited thereto.

[0030] In an embodiment, the insoluble biopolymer is selected from the group consisting of chitin, chitosan, cellulose, hemicellulose, lignin, amylose, actin, fibrin, collagen, silk, silk protein, keratin, wool, and mixtures thereof. In an embodiment, the semi-soluble biopolymer is selected from the group consisting of gelatin, pectin, starch, pullulan, agarose, alginic acid, alginate, hyaluronic acid, RNA, DNA, xanthan gum, guar gum, carrageenan, latex, polymannos, suberin, keratin, horny carbon, and mixtures thereof.

[0031] Biopolymers from various sources can be used, and the present invention is not limited to specific material sources. For example, suitable sources of chitin can include green plants, algae and fungi, but are not limited thereto. Suitable sources of chitin and chitosan can include fungi, crustaceans (such as crabs and shrimps) and insects, but are not limited thereto. In an embodiment, insoluble or semi-soluble biopolymers are obtained from fungi and mushrooms. In an embodiment, insoluble or semi-soluble biopolymers are obtained from plant materials, and the plant materials include roots, tubers, leaves, petals, seeds, fruits, etc., but are not limited thereto. Preferred sources are non-animal sources.

[0032] In an embodiment, the biopolymer is a 100% natural biopolymer, such as SunSpheres Bio TM (microcrystalline cellulose, Dow Chemical), Chemjac TM(konjac root extract and xanthan gum, Chemspire), Kelset TM (sodium alginate, Dupont), Instant Pure Flo F TM (Corn starch, Ingredion), Gelcarin TM GP 379 (carrageenan, iota type, Dupont), Betafib TM MCF (cellulose (and) water-microfibrillated cellulose, Cosun Biobased Products), Betafib TM ETD (cellulose and cellulose gum-microfibrillated cellulose, Cosun Biobased Products), Exilva TM FM02-V,L (cellulose-microfibrillated cellulose, Borregard), Naturesoft TM 800 (cellulose-micro powder), Kelcogel TM CG-HA (Gelan gum-CP, Kelco), agar, agarose, mushroom chitosan (e.g., GBS003, Qingdao Chibio Biotech), fungal chitosan (e.g., GBS010, Qingdao Chibio Biotech; or from Kraeber & Co), chitosan derived from fungi (e.g., Kiosmetine-CS TM , Kitozyme).

[0033] In an embodiment, the biopolymer is a naturally derived biopolymer, such as Natrathix TM Biocellulose (cellulose gum, Ashland), Aquasorb TM A500 (cellulose gum, Ashland), Polysurf TM CS 67 / Natrosol TM CS plus 330 (cetyl hydroxyethyl cellulose, Ashland), Structure XL TM (hydroxypropyl starch phosphate, Nouryon), CD-58 (chitosan succinimide, Onlystar Bio-Technology Ltd), carboxymethyl chitosan derivative (GBS010, Qingdao Chibio Biotech), Makimousse TM 7 / 400 (sodium polyacrylate starch-kobo product, Daito Kasei Kogyo), Salanjul / Sanfresh TM1000 / 300sp (sodium polyacrylate starch, IwaseCosfa USA Inc. / Sanyo), Antaron ECoT ethylcellulose (ethylcellulose, Ashland).

[0034] In embodiments, the biopolymer comprises a synergistic biopolymer combination, such as Chemjac TM (konjac root extract and xanthan gum, Chemspire), PemuPur TM start (microcrystalline cellulose (and) Sphingomonas ferment extract (and) cellulose gum, Lubrizol), Nomcort CG (xanthan gum, carob gum, Ikeda).

[0035] Biopolymers can also include other biopolymer active substances, such as B-CAN TM 55% (oat beta glucan, Adams Food Ingredients) and / or beta glucan from mushrooms.

[0036] The present invention includes a mixture of two, three, four, five or more insoluble biopolymers, including chitin + chitosan, chitin + cellulose, chitin + collagen, chitin + silk, chitosan + silk, chitosan + cellulose, chitosan + collagen, cellulose + collagen, cellulose + silk, collagen + silk, etc., but not limited thereto. The present invention also includes a mixture of two, three, four, five or more semi-soluble biopolymers, including agarose + DNA, xanthan gum + starch, latex + alginate, xanthan gum + DNA, guar gum + horn carbon, etc., but not limited thereto. It is also conceivable to mix two, three, four, five or more insoluble biopolymers and semi-soluble biopolymers together, wherein the insoluble biopolymers and semi-soluble biopolymers include chitin + agarose, chitosan + agarose, chitosan + gelatin, chitosan + xanthan gum, chitosan + xanthan gum, chitosan + sodium hyaluronate, chitosan + sodium hyaluronate, cellulose + sodium hyaluronate, chitosan + agarose, chitosan + agarose, cellulose + agarose, but are not limited thereto.

[0037] The present invention also includes combinations of hydrophobically modified biopolymers and unmodified biopolymers that can form stable viscous oil-in-water emulsions when dispersed using high shear processes and / or mechanical energy (with or without emulsifiers). Examples of hydrophobically modified biopolymers include Natrosol TM CS Plus 330 / Polysurf TM CS 67 (cetyl hydroxyethyl cellulose, Ashland), StarDesign Ultra TM(Sodium starch octenylsuccinate, Cargill Beauty), Inutec TM SP1 (inulin dodecyl carbamate, Beneo), Texturlux Stabil TM (Hydrolyzed corn starch hydroxyethyl ether, Primient).

[0038] In embodiments, biopolymer is made up of biopolymer composition, and the biopolymer composition comprises biopolymer molecules that have been mechanically processed into stable uniform aqueous biopolymer suspension. As used herein, the term "uniform" generally refers to the appearance of suspension under the naked eye (for example, uniform color, uniform texture, etc.). As used herein, uniformity does not exclude the possibility that suspension is "non-uniform" on the molecular level (for example, the presence of various particle sizes, aggregates, etc.). As used herein, the term "stable uniform aqueous biopolymer suspension" or similar terms used interchangeably herein such as "uniform biopolymer suspension" or "stable biopolymer suspension" or simply "biopolymer suspension" all refer to the suspension of insoluble and / or semi-soluble biopolymer particles that have been stably dispersed in polar solvents. Polar solvents can be polar protic solvents or polar aprotic solvents. Polar solvents can be aqueous solvents. Insoluble and / or semi-soluble biopolymer particles present in biopolymer suspensions can be shaped as similar to fibers and / or similar to agglomerated spheres or agglomerates. The stability of biopolymer suspensions can be assessed by any suitable method. In a preferred embodiment, stability is measured or observed by the absence of separation, i.e., there is a single phase rather than two separate phases in the aqueous mixture, e.g., no biopolymer sedimentation / precipitation at the bottom of the aqueous mixture or floating on the top of the aqueous mixture. Preferably, the biopolymer suspension according to the present invention is stable (e.g., without separation) for at least 1 day or at least 1 week or at least one month or at least one year or more.

[0039] Notwithstanding the above, it will be appreciated by those skilled in the art that insoluble and / or semi-soluble biopolymers may never completely dissolve. Instead, they become "swellable" and bind water, which is why the biopolymers become viscous suspensions in accordance with the present invention when subjected to high shear conditions and / or mechanical energy. Thus, the present invention includes "swellable biopolymers" and "non-swellable biopolymers" because non-swellable polymers may be swellable using high shear and / or mechanical energy. As used herein, swellable biopolymers include biopolymers that absorb and bind water such that their particle size and aqueous dispersion viscosity increases.

[0040] In embodiments, the biopolymer may be swollen by wet ball milling. This may include, but is not limited to, chitin, chitosan, hemicellulose, and pregelatinized corn starch.

[0041] In embodiments, the biopolymer may be swollen by a high shear process other than ball milling. This may include, but is not limited to, microcrystalline cellulose, microfibrillated cellulose, nanocellulose, hairy nanocellulose, konjac glucomannan, hydroxypropyl starch phosphate, high acyl gellan gum, gellan gum, carboxymethyl starch, carboxymethyl cellulose (low ds type), agar, and agarose.

[0042] According to the present invention, it is also contemplated to use soluble biopolymers including, but not limited to, xanthan gum, diutangum, sodium alginate, sclerotium gum.

[0043] In an embodiment, the biopolymer molecules or particles as part of the cosmetic composition have been mechanically processed into a stable uniform aqueous biopolymer suspension. In an embodiment, the mechanical processing involves high shear conditions and / or high mechanical energy. In an embodiment, the high shear conditions and / or high mechanical energy are obtained by processes including, but not limited to, mechanical shearing, shear thinning, planetary ball milling, rolling milling, vibratory ball milling, tumbling agitated ball milling, horizontal media milling, colloid milling. As described below, high shear conditions and / or high mechanical energy can be continued for a period of time under parameters, suitable conditions, etc. until a desired state change is obtained, such as a color change, a viscosity change, a change from a slurry to a paste, an ointment, a cream, a lotion, a gel or an emulsion, etc.

[0044] Without wishing to be bound by theory, subjecting the biopolymer to high shear conditions and / or high mechanical energy can improve biopolymer properties that cannot be ensured using conventional methods, including improved rheological properties (e.g., viscosity), shear thinning properties, and high yield values. Biopolymers dispersed by fatty alcohols using a high shear process can contain lamellar crystalline gel networks (LGNs) that can synergistically increase the viscosity of the biopolymer dispersion. The oil + water biopolymer dispersion according to the present invention can also contain a Pickering emulsion, where a water-in-oil or oil-in-water emulsion is stabilized by a biopolymer.

[0045] In an embodiment, high shear conditions and / or high mechanical energy require the use of suitable devices or equipment, including ball mills (e.g., planetary ball mills, rolling mills, vibrating ball mills, tumbling agitated ball mills, horizontal media mills, colloid mills, magnetic mills), twin screw extruders, high pressure homogenizers, blade homogenizers, stirring homogenizers, dispersers, rotor-stator homogenizers, high shear mixers, plowshare mixers, dynamic mixers, plowshare mixers, turbine mixers, speed mixers, friction mills, ultrasonic instruments (e.g., high shear ultrasonic processes), tissue tearers, cell lysers, homogenizers (polytrons), ribbon stirrers, microfluidizers, high pressure homogenizers, and combinations thereof, but are not limited thereto. In a preferred embodiment, the present invention uses ball milling under wet conditions. Specific examples of ball mills include vertical planetary mills (e.g., Tencan XQM-2A) with a 100 mL capacity zirconia jar and 10 mm diameter zirconia balls. TM ), Flacktek with 40mL zirconia canister and 5mm diameter zirconia ball or ring TM Speed ​​mixer (DAC 330-11SE), 1.5L Supermill Plus with 1.4 to 1.7 mm zirconium oxide beads TM and Netzsch Labstar with 0.6 to 0.8 mm beads or 1.4 to 1.7 mm beads, but not limited thereto.

[0046] In a particular embodiment, the biopolymer compositions and suspensions according to the invention are obtained using a particular protocol referred to herein as the "10+1 Alt method". The method comprises grinding the biopolymer for a certain period of time (e.g. 10 minutes), followed by a short pause (e.g. 1 minute), and then grinding in the opposite direction for a certain period of time (e.g. 10 minutes), for a total of 1 hour or 2 hours or 3 hours or 5 hours, 10 hours or 12 hours.

[0047] Advantageously, the viscosity of the composition / suspension can be changed by changing the high shear conditions and / or mechanical energy experienced by the biopolymer. These conditions can be adjusted to obtain a stable uniform suspension (e.g., a stable colloid uniform suspension) with a desired viscosity. Typically, providing more mechanical energy will increase shearing and correspondingly reduce the viscosity of the final product. The biopolymer itself and / or the final composition for drug delivery can be formulated as a paste, ointment, cream, lotion, gel or emulsion.

[0048] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules or particles is a colloidally homogeneous biopolymer suspension. In embodiments, the colloidally homogeneous suspension comprises colloids ranging from about 1 nm to about 1 μm.

[0049] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules comprises a biopolymer fiber. In embodiments, the width of the fiber is from about 1 nm to about 5 μm, or from about 5 nm to about 5 μm, from about 7 nm to about 5 μm, or from about 10 nm to about 5 μm, or from about 20 nm to about 5 μm, or from about 25 nm to about 5 μm, or from about 30 nm to about 5 μm, or from about 35 nm to about 5 μm, or from about 35 nm to about 3 μm. In embodiments, the width of the fiber is at least 1 nm, or at least 5 nm, or at least 10 nm, or at least 20 nm, or at least 30 nm, or at least 40 nm, or at least 50 nm, or at least 75 nm, or at least 100 nm, or at least 250 nm, or at least 500 nm, or at least 750 nm, or at least 1 μm, or at least 2 μm, or at least 3 μm, or at least 4 μm, or at least 5 μm, or wider.

[0050] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules comprises biopolymer fibers having a length of about 1 nm to about 200 μm, about 10 nm to about 100 μm, or about 50 nm to about 10 μm, or about 100 nm to about 10 μm, or about 500 nm to about 10 μm, or about 750 nm to about 10 μm, or about 800 nm to about 10 μm, or about 900 nm to about 5 μm, or about 1 μm to about 10 μm, or about 1 μm to about 5 μm, or about 1 μm to about 3 μm. In embodiments, the length of the fiber is at least 1 nm or at least 10 nm, at least 50 nm or at least 100 nm or at least 250 nm or at least 500 nm or at least 750 nm or at least 800 nm or at least about 900 nm or at least 1 μm or at least 2 μm or at least 3 μm or at least 4 μm or at least 5 μm or at least 6 μm or at least 7 μm or at least 8 μm or at least 9 μm or at least 10 μm or at least 25 μm or at least 50 μm or at least 75 μm or at least 100 μm or at least 150 μm or at least 200 μm or longer. In embodiments, the dry particle size may range from about 1 nm to about 1 μm or up to 10 μm, and the wet particle size may range from about 200 nm to about 20 μm or up to 200 μm.

[0051] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules comprises a biopolymer fiber having: (i) a width greater than 20 nm (e.g., at least 25 nm or at least 40 nm or at least 50 nm) and a length greater than 50 nm (e.g., at least 100 nm or at least 500 nm or at least 1 μm or at least 2 μm); or (ii) a width greater than 32 nm (e.g., at least 35 nm or at least 40 nm or at least 50 nm) and a length greater than 50 nm (e.g., at least 100 nm or at least 500 nm) or (iii) a width greater than 20 nm (e.g., at least 25 nm, at least 40 nm, or at least 50 nm) and a length greater than 500 nm (e.g., at least 600 nm, or at least 750 nm, or at least 1 μm, or at least 2 μm); or (iv) a width greater than 30 nm (e.g., at least 35 nm, or at least 40 nm, or at least 50 nm) and a length greater than 800 nm (e.g., at least 900 nm, or at least 1 μm, or at least 2 μm); or (v) a width greater than 8 nm (e.g., at least 10 nm, or at least 20 nm); or (vi) a width of greater than 11 nm (e.g., at least 15 nm, at least 25 nm, or at least 35 nm, or at least 40 nm, or at least 50 nm) and a length of greater than 166 nm (e.g., at least 200 nm, or at least 350 nm, or at least 500 nm, or at least 750 nm, or at least 900 nm) or at least 1 μm or at least 2 μm; or (vii) a width of greater than 11 nm (e.g., at least 15 nm, or at least 25 nm, or at least 35 nm, or at least 40 nm, or at least 50 nm) and a length of greater than 166 nm (e.g., at least 200 nm, or at least 350 nm, or at least 500 nm, or at least 750 nm, or at least 900 nm) or at least 1 μm or at least 2 μm. or (viii) a width greater than 32 nm (e.g., at least 35 nm, or at least 40 nm, or at least 50 nm) and a length greater than 800 nm (e.g., at least 900 nm, or at least 1 μm, or at least 2 μm, or at least 3 μm, or at least 4 μm, or at least 5 μm, or at least 6 μm, or at least 7 μm, or at least 8 μm, or at least 9 μm, or at least 10 μm, or at least 25 μm, or at least 50 μm, or at least 75 μm, or at least 100 μm, or at least 150 μm, or at least 200 μm, or longer).

[0052] In an embodiment, the biopolymer and / or biopolymer composition comprising biopolymer molecules comprises biopolymer fibers, wherein the average width and average length of the fibers in the composition are as defined above, e.g., the average width is greater than 20 nm (e.g., at least 25 nm or at least 40 nm or at least 50 nm) and the average length is greater than 50 nm (e.g., at least 60 nm, at least 75 nm, or at least 100 nm, or at least 500 nm, at least 750 nm, or at least 1 μm, or at least 2 μm, or at least 3 μm, or at least 4 μm, or at least 5 μm, or at least 6 μm, or at least 7 μm, or at least 8 μm, or at least 9 μm, or at least 10 μm, or at least 25 μm, or at least 50 μm, or at least 75 μm, or at least 100 μm, or at least 150 μm, or at least 200 μm, or more).

[0053] In embodiments, the biopolymer suspension and / or biopolymer composition comprising biopolymer molecules has a pH between about 6.5 and about 8.5. In specific embodiments, the biopolymer suspension is a chitosan suspension having a pH between about 7.8 and about 8.1.

[0054] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules comprises biopolymer fibers of both crystalline regions and amorphous regions. In embodiments, the stable homogenous suspension comprises biopolymer fibers having a spherical shape. In embodiments, the stable homogenous suspension consists primarily of or consists solely of suspended biopolymer nanofibrils.

[0055] Those skilled in the art will appreciate that particle size measurements will vary depending on the measurement method and the state of the particles (e.g., particles in a wet state are generally larger than the same particles in a dry state). Typically, particles are in a wet or suspended state when measured by dynamic light scattering (DLS), and particles are in a dry state when measured by scanning electron microscopy (SEM).

[0056] In embodiments, biopolymers and / or biopolymer compositions comprising biopolymer molecules comprise agglomerated spheres of alginic acid having an average size of about 40 nm to about 80 nm or about 45 nm to about 75 nm when measured by scanning electron microscopy (SEM). In embodiments, stable homogeneous suspensions comprise agglomerated spheres of alginic acid having a median size of about 30 nm to about 70 nm or about 35 nm to about 65 nm, an average size of about 40 nm to about 80 nm or about 45 nm to about 75 nm when measured by scanning electron microscopy (SEM).

[0057] In an embodiment, the biopolymer and / or biopolymer composition comprising the biopolymer molecules comprises agglomerated spheres of cellulose having an average size of about 50 nm to about 80 nm or about 55 nm to about 75 nm, an average size of about 40 nm to about 80 nm or about 45 nm to about 75 nm, when measured by scanning electron microscopy (SEM). In an embodiment, the stable homogenous biopolymer suspension comprises agglomerated spheres of cellulose having a median size of about 35 nm to about 75 nm or about 40 nm to about 65 nm, an average size of about 40 nm to about 80 nm or about 45 nm to about 75 nm, when measured by scanning electron microscopy (SEM).

[0058] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules comprises agglomerated spheres of chitosan having an average size of about 45 nm to about 85 nm or about 50 nm to about 80 nm. In embodiments, the stable homogenous biopolymer suspension comprises agglomerated spheres of cellulose having a median size of about 45 nm to about 80 nm or about 50 nm to about 75 nm when measured by scanning electron microscopy (SEM).

[0059] In embodiments, the biopolymer and / or biopolymer composition comprising the biopolymer molecules comprises agglomerated spheres of chitosan having an average size of about 75 nm to about 120 nm, or about 80 nm to about 115 nm, or about 85 nm to about 110 nm when measured by scanning electron microscopy (SEM). In embodiments, the stable homogeneous suspension comprises agglomerated spheres of chitosan having a median size of about 70 nm to about 100 nm, or about 75 nm to about 95 nm when measured by scanning electron microscopy (SEM).

[0060] In an embodiment, the biopolymer and / or biopolymer composition comprising the biopolymer molecules comprises agglomerated spheres of silk having an average size of about 40 nm to about 165 nm or about 45 nm to about 160 nm when measured by a scanning electron microscope (SEM). In an embodiment, the stable homogenous biopolymer suspension comprises agglomerated spheres of silk having a median size of about 40 nm to about 150 nm or about 45 nm to about 140 nm when measured by a scanning electron microscope (SEM).

[0061] In an embodiment, the biopolymer and / or biopolymer composition comprising biopolymer molecules comprises particles of one or more of alginate, cellulose, chitin, chitosan and silk, wherein the particle size range when measured by SEM is as defined in the tables and figures of WO 2022 / 137184.

[0062] In embodiments, the biopolymer and / or biopolymer composition comprising the biopolymer molecules is characterized by those visual properties as shown by the SEM images shown in the figures of WO 2022 / 137184.

[0063] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules is characterized by a Fourier transform infrared spectroscopy (FTIR) spectrum as shown in the figures of WO 2022 / 137184.

[0064] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules is characterized by solid state nuclear magnetic resonance (SSNMR) characterization as shown in the figures of WO 2022 / 137184.

[0065] In an embodiment, the biopolymer and / or biopolymer composition comprising the biopolymer molecules is characterized by a Power X-Ray Diffraction (PXRD) pattern as shown in the figures of WO 2022 / 137184.

[0066] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules is characterized by dynamic light scattering (DLS) measurements as those described in WO 2022 / 137184.

[0067] In embodiments, the biopolymer and / or biopolymer composition comprising biopolymer molecules is characterized by a transmission spectrum as shown in the Figures of WO 2022 / 137184.

[0068] In an embodiment, the biopolymer and / or biopolymer composition comprising biopolymer molecules is characterized by the Glance Suspension Test as described in WO 2022 / 137184.

[0069] In embodiments, the biopolymer and / or biopolymer composition comprising the biopolymer molecules is characterized by rheological behavior as shown in the figures of WO 2022 / 137184.

[0070] Sunscreen composition

[0071] A particular aspect of the invention relates to a sunscreen composition comprising a biopolymer and / or a biopolymer suspension as described herein. In an embodiment, the biopolymer comprises or consists of a biopolymer composition comprising biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension (e.g., a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent).

[0072] Advantageously, the sunscreen composition may comprise smaller monomeric units of multimeric biopolymers, such as oligomers of acetylglucosamine (GlcNAc) and / or NAG, which may exhibit UV-shielding properties.

[0073] In one embodiment, the sunscreen composition comprises: a biopolymer, and at least one of a sensory enhancer, a film former, an emulsifier, a co-emulsifier, an emollient, a UVB filter, a UVA filter, a broad spectrum filter, and a preservative. In an embodiment, the sunscreen composition comprises at least water, a biopolymer (acting as a thickener), a UVB absorber, a UVA absorber, an emollient, and a preservative. In an embodiment, the sunscreen composition further comprises one or more of the following optional ingredients: a humectant, an emulsifier, and a film former.

[0074] In an embodiment, the sensory enhancer is selected from cyclopentasiloxane, cyclohexasiloxane, cyclopentasiloxane, cisiloxane, trisiloxane, dimethicone, caprylyl methicone, phenyl trimethicone, cetyl dimethicone, bis-hydroxyethoxypropyl dimethicone, dimethicone / vinyl dimethicone crosspolymer, dimethicone / dimethicone crosspolymer, PEG-20 sorbitan cocoate, phenoxyethanol, ethylhexylglycerin, potassium sorbate, phenoxyethanol, methylparaben, isopropylparaben, isobutylparaben, butylparaben, and mixtures thereof.

[0075] In an embodiment, the film former is selected from the group consisting of cetyl dimethicone, stearyl dimethicone, phenyl trimethicone, bis-hydroxyethoxypropyl dimethicone, isododecane, polypropyl silsesquioxane, dimethicone / vinyl dimethicone crosspolymer, C30-45 alkyl methicone, C30-45 olefin, beeswax, candelilla (euphorbia cerifera) (candelilla) wax, copernicia prunifera) (carnuba) wax, isododecane, acrylates polytrimethylsiloxy methacrylate copolymer, hydrogenated polycyclopentadiene, galactoarabinan, dihydroabietate, trimethylsiloxy silicate, trimethylsiloxy silicate, polypropyl silsesquioxane, C30-45 alkyl dimethylsilyl polypropyl silsesquioxane, ethylenediamine / hydrogenated dimer dilinoleate copolymer bis-di-C14-18 alkyl amide, acrylate copolymers, and mixtures thereof. Specific examples include Dermacryl E TM (Styrene / acrylate copolymer (Nouryon)), Ganex TM V220 (eicosene / VP), Cosmedia TM DC (hydrogenated dimer linoleyl carbonate / dimethyl carbonate (BASF)), Baycusan TM C 1000 (Polyurethane-34 (Covestro)), Hercolyn TM D (Methyl Hydrogenated Rosinate (Pinova)), Tego TM SP 13-1, 13-6 (poly C10-30 alkyl acrylate (Evonik)), Ganex TM P904 (Butyl PVP (Ashland)), but not limited thereto.

[0076] In an embodiment, the emulsifier is selected from acrylates / C10-30 alkyl acrylate crosspolymer, potassium cetyl phosphate, sodium cetearyl sulfate, C20-22 alkyl phosphate, PEG-30 dipolyhydroxystearate, polyglyceryl-2-dipolyhydroxystearate, polyglyceryl-3-diisostearate, and mixtures thereof. Specific examples include Emulsiphos TM(Potassium Cetyl Phosphate + Hydrogenated Palm Glycerides (Symrise)), Dracorin TM GOC (Glyceryl Oleate Citrate + Caprylic / Capric Triglyceride (Symrises)), Dracorin TM CE (glyceryl stearate citrate (Symrise)), polyglyceryl 10 stearate and glyceryl tristearate, but are not limited thereto.

[0077] In an embodiment, the co-emulsifier is selected from cetyl alcohol, stearyl alcohol, stearic acid, glyceryl stearate, sorbitan stearate, polyglyceryl 3 distearate and mixtures thereof. In an embodiment, the emollient is selected from C12-15 alkyl benzoate, trisiloxane, squalene, butyloctanol salicylate, isopropyl isostearate, isostearyl isostearate, octyl carbonate, isopropyl myristate, dibutyl adipate, caprylic / capric triglyceride, coconut caprylate, dicaprylyl carbonate, ethylhexyl methoxycrylene, diethylhexyl 2,6-naphthalene dicarboxylate, butyloctanol salicylate, tridecyl salicylate, polyester-8, diethylhexyl syringylidenemalonate, benzotriazolyl dodecyl p-cresol, propyl heptyl octanoate and mixtures thereof.

[0078] In an embodiment, the UVB filter is selected from Uvivul TM T 150 (ethylhexyl triazone), titanium dioxide, benzophenone-3 (oxybenzone), octinoxate (ethylhexyl methoxycinnamate), octocrylene, ethylhexyl salicylate (octisalicylate), homosalate (homomenthyl salicylate), ensulizole (phenylbenzimidazole sulfonic acid), amiloxate (isoamyl p-methoxycinnamate), polysilicone-15, diethylhexyl butamido triazone (iscotrizinol), 4-methylbenzylidene camphor (enxacamene), and mixtures thereof.

[0079] In an embodiment, the UVA filter is selected from butyl methoxydibenzoylmethane, butyl methoxydibenzoylmethane (Avobenzone TM ), diethylamino hydroxybenzoyl hexyl benzoate (Univul TM a plus), terephthalylidene dicamphor sulfonic acid (Mexoryl TM SX), disodium phenyl dibenzimidazole tetrasulfonate (Neo Heliopan TMap), Tinosorb TM S (Bis-ethylhexyloxyphenol methoxyphenyl triazine), Tinosorb TM M (methylene bis-benzotriazolyl tetramethylbutylphenol (and) water (and) decyl glucoside (and) propylene glycol (and) xanthan gum), Tinosorb TM A2B (tris-biphenyl triazine (and) water (and) decyl glucoside (and) butylene glycol (and) disodium phosphate (and) xanthan gum) and mixtures thereof.

[0080] In an embodiment, the broad spectrum filter is selected from bis-ethylhexyloxyphenol methoxyphenyl triazine, tris-biphenyl triazine (nano), methylene bis-benzotriazolyl tetramethylbutylphenol, drometrizole trisiloxane, zinc oxide and mixtures thereof.

[0081] In an embodiment, the SPF booster is selected from Soltex INO TM Polymer (acrylates / methacryloyloxyethyl phosphate copolymer, Dow Chemical), Soltex INO TM Polymer (acrylates / methacryloyloxyethyl phosphate copolymer, Dow Chemical), Solterra Boost TM (Methylcellulose, Dow Chemical), Synoxyl HSS TM (Trimethoxybenzylidene pentanedione, Sytheon), SunSpheres TM Powder (styrene acrylate copolymer, DowChemical), SunSpheres BIO TM SPF booster (microcrystalline cellulose, Dow Chemical), SunBoost ATB TM (Argan Oil (and) Tocopheryl Acetate (and) Bisabolol, Kobo Products), NS Boost TM (3% active substance), NSBoost TM (Mica, Silica, Next Step Labs), LaraCare TM A200 (arabinogalactan, Lonza), HallBrite TM BHB (Butyl Octyl Salicylate, Hallstar), Dow Corning TM 5562 Methanol Liquid (Bis-Hydroxyethoxypropyl Dimethicone, Nexeo Solutions), Performacol TM350 (C20-40 alcohol, New Phase Technologies), Corapan TM TQ (diethylhexyl 2,6-naphthalene dicarboxylate, Symrise), Lexgard TM O (caprylyl glycol, Inolex), HallBrite TM BHB (butyl octanol salicylate, Hallstar), TDSA (tridecyl salicylate, Alzo Inc.), Karansun TM SC 50 Concentrate (Pongamia Seed Oil, BioSynthis), Pongamia Butter (Pongamia Extract, Biosynthis), Solastay TM S1 (ethylhexyl methoxy polyacrylonitrile, Hallstar), AvoBrite TM (Acrylates Copolymer, Hallstar), Tego TM SP 13-1, 13-6 (poly C10-30 alkyl acrylate, Evonik), Oleocraft TM HP 31, MP30, MP31 (polyamide 3, Croda), Koboguard TM HRPC (Hydrogenated Polycyclopentadiene (and) Polyethylene (and) Copernicia cerifera (Carnauba) Wax (and) Tocopherol, Kobo Products), Baycusan TM C1000 (Polyurethane 34, 40% latex solids, Bayer Material Science), SolPerForm TM (water, hydrolyzed wheat protein / PVP crosspolymer, 29% solids, Croda) and mixtures thereof.

[0082] In an embodiment, the preservative is selected from the group consisting of benzyl alcohol, sodium benzoate, caprylyl glycol, glyceryl caprylate, phenoxyethanol, hexylene glycol, hydroxyacetophenone, sodium dehydroacetate, sodium phytate, and mixtures thereof.

[0083] In an embodiment, the biopolymer functions as a thickener. However, it is contemplated that a thickener may be added to the sunscreen composition, including, but not limited to, cetyl alcohol, stearyl alcohol, carnauba wax, stearic acid, hydroxyethylcellulose, locust bean gum, xanthan gum, gelatin, silica, bentonite, magnesium aluminum silicate, carbomer, sodium chloride.

[0084] In an embodiment, the sunscreen composition comprises the following ingredients:

[0085]

[0086] In embodiments, the sunscreen composition provides a sun protection factor (SPF) of at least 15, or at least 30, or at least 45, at least 50, or at least 60.

[0087] The sunscreen composition according to the present invention can have many applications, at least for protecting the skin from sunlight. The method of use may include simply applying the composition to the skin. The application may be repeated as needed, for example at different time intervals (e.g., every hour, or every 2 hours, or every 6 hours, or every 12 hours, or once a day, or twice a day, or three times a day, or once, twice or three times a week, etc.).

[0088] Skin care compositions

[0089] A particular aspect of the invention relates to a skin care composition comprising a biopolymer and / or a biopolymer suspension as described herein. In an embodiment, the biopolymer comprises or consists of a biopolymer composition comprising biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension (e.g., a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent).

[0090] Advantageously, the skin care composition may comprise smaller monomeric units of multimeric biopolymers, such as oligomers of N-acetylglucosamine (GlcNAc) and / or NAG, which may exhibit anti-aging properties.

[0091] In one embodiment, the skin care composition comprises: a biopolymer, and at least one of a moisturizer, an emollient, an occlusive, a ceramide, an emulsifier, a thickener, a preservative, an antioxidant, a fragrance, and a functional ingredient. In an embodiment, the skin care composition comprises at least a biopolymer, an emulsifier / stabilizer, and a preservative.

[0092] In an embodiment, the moisturizer is selected from glycerin, urea, hyaluronic acid, salicylic acid, alpha hydroxy acid, propylene glycol, honey, sorbitol, butylene glycol, sodium PCA, allantoin, sodium lactate, dicyandiamide, Tremella fuciformis extract, seaweed, aloe vera, and mixtures thereof.

[0093] In an embodiment, the emollient is selected from beeswax, butylphthalimide, isopropylphthalimide, Euphorbia cera, castor, cocoa butter, coconut oil, cyclomethicone, cyclopentasiloxane, dicaprylyl ether, diisopropyl dimer dilinoleate, dimethicone, dipropylene glycol dibenzoate, glycerin, hydrogenated polyisobutene, isocetyl stearoyl stearate ... stearate), isododecane, isohexadecane, isoparaffin, isopropyl isostearate, isopropyl palmitate, isopropyl stearate, jojoba oil, lanolin, mineral oil, octyldodecanol, octyl palmitate, olive oil, palm, pentaerythrityl tetraoctanoate, petrolatum, phenethyl benzoate, polyglyceryl-4 oleate, propylene glycol, propylene glycol dibenzoate, propylene glycol isostearate, shea butter, sorbitol, squalane, sunflower, trisiloxane, and mixtures thereof.

[0094] In an embodiment, the occlusive agent is selected from the group consisting of mineral oil, petrolatum, lanolin, dimethicone, shea butter, and mixtures thereof.

[0095] In an embodiment, the ceramide is selected from the group consisting of ceramide EOS, ceramide NS, ceramide NG, ceramide NP, ceramide AP, ceramide EOP, phytosphingosine, sphingosine, and mixtures thereof.

[0096] In an embodiment, the emulsifier is selected from the group consisting of sodium lauryl sulfate, dioctyl sodium sulfosuccinate, tragacanthins, lecithin, mustard, soy lecithin, sodium phosphate, diacetyl tartaric acid monoglyceride, sodium stearoyl lactylate, and mixtures thereof.

[0097] In an embodiment, the thickener is selected from the group consisting of magnesium aluminum silicate, silica, bentonite, carbomer, cetyl palmitate, ammonium acryloyldimethyltaurate, and mixtures thereof.

[0098] In an embodiment, the preservative is selected from phenoxyethanol, benzyl alcohol, sodium benzoate, potassium sorbate, ethylhexylglycerin, DMDM ​​hydantoin, quaternium-15, imidazolidinyl urea, diazolidinyl urea, polyoxymethylene urea, sodium hydroxymethylglycinate, 2-bromo-2-nitropropane-1,3-diol (bromopol), glyoxal, ethylparaben, butylparaben, methylparaben, propylparaben, isobutylparaben, isopropylparaben, benzyl, benzoic acid, benzyl ester, methylisothiazolinone (MIT; 2-methyl-4-isothiazolin-3-one), methylchloroisothiazolinone (CMIT; 5-chloro-2-methyl-4-isothiazolin-3-one), phenoxyethanol, 2-phenoxyethanol, Euxyl K TM 400 (a mixture of phenoxyethanol and 1,2-dibromo-2,4-dicyanobutane), triclosan (TSC), triclocarban (tcc), benzyl alcohol, benzalkonium chloride, citric acid, dehydroacetic acid, essential oils, grapefruit seed extract, lactic acid, levulinic acid, potassium sorbate, sodium dehydroacetate, sodium metabisulfite, sodium salicylate, sorbic acid, vitamin E, zinc pyrithione, and mixtures thereof. In an embodiment, the preservative comprises Dermosoft TM and Spectrastat TM At least one of .

[0099] In an embodiment, the antioxidant is selected from vitamin A, vitamin C, vitamin E, vitamin B3, green tea extract, resveratrol, curcumin, lycopene, coenzyme Q10, polyphenols, C. asiatica, tamanu, and mixtures thereof.

[0100] In an embodiment, the fragrance is selected from linalool, citronellol, limonene, geraniol, eugenol, lavender oil (Lavandula angustifolia), rose flower extract (Rosa damascene, canina, centifolia, gallica, multiflora), bergamot oil (Citrus bergamia), ylang-ylang oil (Canaga odorata), lemon (Citrus limon), lime (Citrus aurantifolia or Citron (Citrus medica), orange (Citrus sinensis), citrus (Citrus tangerine), peppermint (Mentha piperita), spearmint (Mentha odorata), spicata), eucalyptus, cinnamon bark (Cinnamomum), cinnamaldehyde (cinnamal), and mixtures thereof.

[0101] In an embodiment, the functional ingredient is selected from biological extracts, vitamins, amino acids, peptides, proteins, essential fatty acids (eg, alpha hydroxy acids, beta hydroxy acids), beta-glucans, and mixtures thereof.

[0102] Skin care compositions according to the present invention can have many applications, at least benefiting the skin. For example, the skin care composition can provide one or more of the following benefits: moisturizing the skin, improving skin appearance, protecting from environmental damage, combating the effects of aging, removing wrinkles and / or sun spots.

[0103] In an embodiment, the skin care composition comprises the following ingredients:

[0104]

[0105] Methods of use may include simply applying the composition to the skin. Can be repeated as needed, for example at different time intervals (e.g., every hour, or every 2 hours, or every 6 hours, or every 12 hours, or once a day, or twice a day, or three times a day, or once, twice or three times a week, etc.).

[0106] In embodiments, the skin care compositions according to the present invention can be used to "smooth" the skin (e.g., remove wrinkles). In embodiments, the skin care composition reduces the average Ra parameter (linear average profile roughness) by at least 0.1% or at least 0.25% or at least 0.5% or at least 1% or at least 1.5% or at least 2% or at least 2.5% or at least 3% or at least 3.5% or at least 4% or at least 4.5% or at least 5% or between 0.5% and 5% or between 1% and 3% or about 2.5% of the average value 15 minutes after application to the skin of a human subject. In other embodiments, the skin care composition reduces the average Ra parameter by the same values ​​described above after 10 days of use (applied once, twice, or three times per day on the skin for 10 days).

[0107] In an embodiment, the skin care composition according to the present invention has a water resistance effect, as measured after 10 sprays of water from the application of the product. In an embodiment, the skin care composition has a water resistance score greater than or equal to 7, such as at least 7 or at least 7.5 or at least 8 or at least 8.5 or at least 9 or at least 9.5.

[0108] In an embodiment, the skin care composition according to the present invention is dermatologically safe. In an embodiment, the composition does not cause any adverse effects, such as erythema, peeling, edema and blisters, when applied to the skin. Preferably, the skin care composition according to the present invention shows a very low or zero mean irritation index (MII) (e.g., MII of 0 at 15 minutes and 24 hours after application according to a conventional patch test (see Example 9 below)). Preferably, the skin care composition according to the present invention can be classified as "non-irritating" and "dermatologically tested as skin compatible".

[0109] In an embodiment, according to USP regulations (e.g., USP 51 and / or USP 61), the skin care composition according to the present invention can fully meet the requirements of the preservative efficacy test for topical products. Preferably, the skin care composition can reduce the total number of microorganisms of various microbial strains (including Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC6538, Candida albicans ATCC 10231, and Aspergillus brasiliensis ATCC 16404, but not limited thereto). Preferably, the skin care composition effectively reduces the total number of microorganisms by at least 1 log, or at least 2 log, or at least 3 log, or at least 4 log, or at least 5 log over 14 days. In embodiments, the skin care composition may be suitably preserved to prevent bacterial growth for at least 5 days, or at least 10 days, at least 14 days, at least 21 days, at least 28 days, or longer.

[0110] Hair care composition

[0111] A particular aspect of the invention relates to a hair care composition comprising a biopolymer and / or a biopolymer suspension as described herein. In an embodiment, the biopolymer comprises or consists of a biopolymer composition comprising biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension (e.g., a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent).

[0112] In one embodiment, the hair care composition comprises: a biopolymer, and at least one of a surfactant, a hair conditioning surfactant, a co-surfactant, a cationic conditioning polymer, a conditioning softener, a thickener, an opacifier, a ceramide, a conditioning agent, a pH buffer, a chelating agent, a preservative, an antioxidant, a fragrance, and a functional ingredient. In an embodiment, the skin care composition comprises at least a biopolymer, an emulsifier, a stabilizer, and a preservative.

[0113] In an embodiment, the biopolymer is selected from the group consisting of chitin, chitosan, cellulose, and mixtures thereof. In an embodiment, the biopolymer is microcrystalline cellulose (e.g., SunSpheres TM BIO SPF booster), microfibrillated cellulose (such as Exilva TM (FM02-V), Borregaard). It is also conceivable to use mixtures of these celluloses.

[0114] In an embodiment, the surfactant is selected from lauryl sulfates (e.g., sodium lauryl sulfate), laureth sulfate salts (e.g., sodium laureth sulfate), sarcosinates (e.g., sodium lauroyl sarcosinate), sulfosuccinates (e.g., sodium dioctyl sulfosuccinate), betaines (e.g., cocamidopropyl betaine), alkyl amino acids, poloxamers, amine oxides (e.g., cocamidopropyl amine oxide), polyglucosides (e.g., dodecyl glucoside), and mixtures thereof.

[0115] In an embodiment, the conditioning surfactant is selected from the group consisting of behenyltrimonium chloride, stearyl dimethylaminopropylamine, cetyltrimonium chloride, stearalkonium chloride, and mixtures thereof.

[0116] In an embodiment, the cationic conditioning polymer is selected from the group consisting of Polyquaternium 7, 10, 53, 87, guar hydroxypropyltrimonium chloride, starch hydroxypropyltrimonium chloride, Sensomer TM CI-50 (33% active, Lubrizol), Cassia gelatin hydroxypropyltrimonium chloride, and mixtures thereof.

[0117] In an embodiment, the co-surfactant is selected from the group consisting of cetyl alcohol, stearyl alcohol, glyceryl stearate, and mixtures thereof.

[0118] In an embodiment, the conditioning softener is selected from dimethicone, dimethiconol, trisiloxane, amodimethicone, polyricinoleic acid, polyester 37, trimethylolpropane tricaprylate / tricaprate, polyester-11, PPG-3 benzyl etherethylhexanoate, and mixtures thereof.

[0119] In an embodiment, the preservative is selected from caprylyl glycol, hexylene glycol, ethyllauroyl arginate HCl, Everguard TM LAE 20 (20% solution in glycerin), benzyl alcohol, glyceryl caprylate, trisodium ethylenediamine disuccinate, sodium benzoate, potassium sorbate, Euxyl TM k 712 preservatives (sodium benzoate (and) potassium sorbate (and) water-ashland) and mixtures thereof.

[0120] In embodiments, the thickener is selected from the group consisting of sodium chloride, gums, cellulose, polyvinyl alcohol, acrylate copolymers, and mixtures thereof.

[0121] In an embodiment, the preservative is selected from isothiazolones (eg, methylchloroisothiazolinone), cationic surfactants (eg, benzalkonium chloride), and mixtures thereof. Further examples of possible preservatives and possible stabilizers and / or emulsifiers are provided below.

[0122] In an embodiment, the opacifying agent and pearlescent agent are selected from the group consisting of polyglycolester, latex opacifier, pearlescent color additive, and mixtures thereof.

[0123] In an embodiment, the conditioning agent is selected from quaternary surfactants (e.g., stearalkonium chloride, cetyltrimonium chloride, quaternary ammonium salts and polyquaternium salts (e.g., polyquaternium-10)), polyvinylpyrrolidone, humectants (e.g., glycerin), proteins, silicones (e.g., dimethicone, cyclomethicone, dimethicone and amodimethicone), and mixtures thereof.

[0124] In embodiments, the pH buffer is selected from the group consisting of citric acid, glycolic acid, and mixtures thereof.

[0125] In an embodiment, the chelating agent is EDTA.

[0126] In an embodiment, the active ingredient is selected from anti-dandruff agents (eg, ketoconazole) zinc pyrithione, sulfur, salicylic acid, and mixtures thereof.

[0127] In an embodiment, the additional ingredients are selected from vitamins, flavors, plant extracts, and mixtures thereof.

[0128] The hair care composition according to the present invention can be used in many applications, such as in shampoo, rinse-off cream conditioner, spray conditioner, shower gel and / or any other product for washing hair, preventing and / or treating hair damage, etc. The method of use can include simply applying the composition to the hair (and / or body) during washing, rinsing with water and drying the hair (and / or). The application can be repeated as needed, for example at different time intervals (e.g. once a day, or twice a day, or once, twice or three times a week, etc.).

[0129] In an embodiment, the hair care composition is formulated as a shampoo (e.g., a conditioning shampoo) and comprises the following ingredients:

[0130]

[0131] In an embodiment, the hair care composition is formulated as a rinse-off cream conditioner and comprises the following ingredients:

[0132]

[0133] In an embodiment, the hair care composition is formulated as a spray conditioner and comprises the following ingredients:

[0134]

[0135] The nature of expectations

[0136] The cosmetic composition according to the invention is preferably stable. Stable means at least that the biopolymer (e.g. fibers, spheroids) or any other component of the composition does not settle to the bottom. In an embodiment, the insoluble and / or semi-soluble biopolymer remains in suspension for at least 1 week or at least 1 month or at least 6 months or at least 12 months or at least 18 months or at least two years or at least three years or longer.

[0137] The cosmetic composition according to the present invention can be formulated to have a desired viscosity (e.g., the viscosity of a so-called paste, ointment, cream, lotion, gel or emulsion). In embodiments, the viscosity of the composition / suspension can be varied by varying the high shear conditions and / or mechanical energy to which the biopolymer is subjected. In embodiments, a stable homogeneous suspension has a viscosity of about 25 mPa to about 85,000 mPa. Table 1 below provides non-limiting examples of desired viscosities of compositions / suspensions according to the present invention.

[0138] Table 1 : Example of desired viscosity

[0139] Required viscosity Exemplary viscosity (mPa·s) Paste About 40,000 to about 300,000 ointment About 20,000 to about 50,000 Cream About 1500 to about 30000 lotion About 800 to about 4000 gel About 1,000 to about 40,000 Lotion About 20 to about 2000

[0140] In embodiments, the viscosity of the skin care and / or sunscreen compositions according to the invention may be varied by selecting sensory enhancers, film formers, emulsifiers, emollients, UVB filters, UVA filters and / or broad spectrum filters accordingly.

[0141] In addition to the biopolymer, the viscosity of the cosmetic composition according to the present invention can also be varied depending on the ratio or concentration of each compound entering the composition. In an embodiment, the weight ratio of biopolymer: other components (e.g., sensory enhancers, film formers, emulsifiers, emollients, UVB filters, UVA filters and / or broad spectrum filters, etc.) is from about 0.1:20 to about 10:20, or from about 0.5:20 to about 3:20, or about 0.75:20, or about 1.0:20, 1.25:20, or about 1.5:20. In an embodiment, the cosmetic composition according to the present invention comprises from about 0.01% w / w to 10% w / w of biopolymer, or from 0.01% w / w to 5% w / w of biopolymer, or from 0.01% w / w to 2% w / w of biopolymer, or from 0.01% w / w to 1% w / w of biopolymer. In an embodiment, the cosmetic composition according to the present invention comprises about 0.01% w / w of biopolymer, or about 0.05% w / w of biopolymer, or about 0.1% w / w of biopolymer, or about 0.25% w / w of biopolymer, or about 0.5% w / w of biopolymer, or about 0.75% w / w of biopolymer, or about 1% w / w of biopolymer, or about 1.5% w / w of biopolymer, or about 2.5% w / w of biopolymer, or about 5% w / w of biopolymer.

[0142] The cosmetic composition according to the invention may also be formulated as a stable emulsion comprising fatty acids (e.g. C 10 -C 22 fatty acids), oils and / or waxes, and / or contain N-acetylglucosamine, and / or emulsifiers and preservatives, and / or additives.

[0143] A non-limiting list of additives includes preservatives, stabilizers and emulsifiers (e.g., cetyl alcohol, glyceryl stearate, soybean oil, PC90, tara gum, PSC3, PEG, guar gum, xanthan gum, agarose, sodium hyaluronate, Tween 80 TM, glycerin (humectant)), thickeners, dyes, powders (e.g. mica, pigments, chalk), inks, colorants, fragrances, essential oils, extracts (e.g. plant extracts such as aloe vera), vitamins (e.g. ascorbic acid), acids (e.g. acetic acid, citric acid, stearic acid), oils (cocoa butter, emu oil, olive oil, shea butter, silicone oil, mineral oil), metal oxides (e.g. zinc oxide), salts (e.g. sea salt, sodium lactate), honey, clay, propylene glycol, polyethylene glycol, dry ingredients (e.g. rose petal powder, orange peel powder, chamomile flower, calendula petals, etc.), allantoin, acetylglucosamine ( GlcNAc), waxes (e.g., beeswax), peptides and proteins, pharmaceutical compounds (e.g., N-acetylglucosamine, lidocaine, capsaicin, baclofen, ketamine, methylsulfonylmethane, o-phenadrine, tetracaine, amitriptyline, bupivacaine, cyclobenzaprine, doxepin, gabapentin, guaifenesin, acetaminophen, ibuprofen, naproxen, diclofenac, meloxicam, piroxicam, ketoprofen, any nonsteroidal anti-inflammatory drug), sugars (e.g., glucose, fructose, galactose, etc.), monomers of any one of cellulose, starch, chitin, chitosan, alginic acid, collagen, silk, etc., but not limited thereto. Additives may be added before, during, and / or after the step of high shear conditions and / or high mechanical energy.

[0144] In an embodiment, the additive or stabilizer is selected from the following stabilizers: agar, sodium alginate, carrageenan, guar gum, konjac, tragacanth gum, locust bean gum, psyllium, tara gum, fenugreek gum, xanthan gum, abietic acid, acetyl mannosyl erythritol esters, acrylamide / sodium acryloyldimethyl taurate copolymer, acrylates / aminoacrylates / C10-30 alkyl peg-20 itaconate copolymer, acrylates / C10-30 alkyl acrylate crosspolymer, acrylates / C5-8 alkyl acrylate copolymer, acrylates / octadecyl methacrylate copolymer, acrylates / vinyl isodecanoate crosspolymer, acrylates / vinyl neodecanoate crosspolymer, acrylic acid / octadecyl acrylate copolymer, acrylic acid / octadecyl methacrylate / polydimethylsiloxane methacrylate copolymer, bisacryloyl poloxamer, Alcaligenes polysaccharide, alcohol C9-11 , Allyl Methacrylate Crosspolymer, Polyglyceryl-4 Sweet Almond Oil, Aluminum Behenate, Aluminum Octanoate, Aluminum Dicetyl Phosphate, Aluminum Dilinoleate, Aluminum Dimyristate, Aluminum Distearate, Aluminum Isostearate, Aluminum Isostearate / Laurate / Palmitate, Aluminum Isostearate / Laurate / Stearate, Aluminum Isostearate / Myristate, Aluminum Isostearate / Palmitate, Aluminum Isostearate / Stearate, Aluminum Lanolinate, Aluminum Monostearate, Aluminum Myristate, Myristic Acid / Palmitate Aluminum, aluminum hydroxide / magnesium stearate, ammonium acryloyldimethyltaurate / steareth-25 methacrylate crosspolymer, ammonium acryloyldimethyltaurate / steareth-8 methacrylate copolymer, ammonium acryloyldimethyltaurate / vinyl formamide copolymer, ammonium alginate, ammonium phosphatidyl rapeseed oil, ammonium polyacryloyldimethyltaurate, ammonium lacquerate, amodimethicone glyceryl carbamate glycerocarbamate), AMP-C8-18 perfluoroalkyl ethyl phosphate, water blue algae polysaccharide, arachidyl alcohol, gum tragacanth, gum tragacanth root extract, avocado amide DEA, babassu acid, cross-linked bacillus / glucose / sodium glutamate ferment, right-handed, left-handed batyl alcohol, hydrolyzed beeswax, synthetic beeswax, behenyl alcohol, bentonite, benzalkonium montmorillonite, benzalkonium sepiolite, bittern emulsion stabilizer, brassicol emollient, brassicyl isoleucinate esylate, butylene glycol / vinyl alcohol copolymer, butoxyhydroxypropyl cetyl hydroxyethylcellulose, butter decyl esterester), butyl acrylate / isopropyl acrylamide / peg-18 dimethacrylate crosspolymer, butyl babassu oleate, butylene glycol cocoate, butylene glycol isostearate, C1-5 alkyl galactomannan, C12-13 alcohol, C12-14 sec-alcohol pareth-3, C12-14 sec-alcohol pareth-5, C12-14 sec-alcohol pareth-7, C12-14 sec-alcohol pareth-8, C12-14 sec-alcohol pareth-9, C12-14 sec-alcohol pareth-12, C12-14 sec-alcohol pareth-15, C12-14 sec-alcohol pareth-20, C12-14 sec-alcohol pareth-30, C12-14 sec-alcohol pareth -40, C12-14 sec-Alkyl Eth-50, C12-15 alcohol, C12-16 alkyl peg-2 hydroxypropyl hydroxyethylcellulose, C12-18 alkyl glucoside, hydrogenated C12-18 triglycerides, C14-15 alcohol, C14-18 glycol, C14-22 alcohol, C15-18 glycol, bis-C16-18 alkyl glyceryl undecyl polydimethylsiloxane, C18-22 alkyl peg-25 methacrylate / diethylaminoethyl methacrylate copolymer, C18-30 glycol, C18-38 alkyl hydroxystearoyl stearate, C20-22 alcohol, C20-30 glycol, C20-40 alcohol, C20-40 alkyl alcohol (C20-40 C22-24 Pareth-33, Bis-C24-28 Hydroxyalkyl Olivate Glutamate, C28-52 Olefin / Undecylenic Acid Copolymer, C30-50 Alcohol, Calcium Carboxymethylcellulose, Calcium Carrageenan, Calcium Laurate, Calcium Myristate, Calcium Polyglutamate Crosspolymer, Calcium Carbomer, Calcium Sucrose, Calcium Octenylsuccinate Starch, Calcium Stearate, Cypress Resin, Euphorbia Cerifera Wax, Euphorbia Cerifera Wax, Euphorbia Cerifera Wax / Jojoba / Rice Bran Polyglyceryl-3 Esters, Caprylyldimethicone Ethoxy Glucoside, Caprylyl / Capryl Wheatbran / Straw Glycoside glycoside), carbomer 934, carboxymethylcellulose acetate butyrate, carboxymethyl hydroxyethylcellulose, carboxymethyl hydroxypropyl guar gum, carnauba wax, safflower oleosomes, hydrogenated castor oil behenyl esters, castor oil phosphate, hydrogenated castor oil stearyl esters, hydrogenated castor oil / sebacic acid copolymer, caprate / caprylate, cellulose acetate propionate carboxylate, hydrolyzed cellulose gum, microcrystalline cellulose, ceramide NS / peg-8 / succinic acid copolymer, carob bean gum, ozokerite, ceteareth-6 olive oil ester, cetearyl alcohol, cetyl alcohol, cetyl dimethicone peg7 acetate, cetyl dodecenyl succinate, cetyl hydroxyethylcellulose, cetyl peg / ppg-7 / 3 polydimethylsiloxane, bis-cetyl / peg-8 cetyl peg-8 polydimethylsiloxane, chitosan lauramide succinimide, chitosan lauroyl glycinate, cholesterol / hdi / pullulan copolymer, sweet orange peel extract, snow orange fiber (citrus aurantium sinensis fiber), cocamide, cocamide DEA, cocamide MEA, cocamide MIPA, cocamidopropyl lauryl ether, cocoa butter glycerides, coconut alcohol, coconut oil methylpropylene glycol esters, hydrolyzed corn starch hydroxyethyl ether, guar gum, decyl ricinoleate, decyl glucoside, 7-dehydrocholesterol, dehydroxanthan gum, dicaprylylsodium sulfosuccinate sulfosuccinate), diethylene glycol / hydrogenated dimer dilinoleic acid copolymer, digalactosylglyceryl linoleate / palmitate / oleate, diglyceryl / dilinoleic acid / hydroxystearic acid copolymer, dihydrolanosterol, dihydroxyethyl cocamine oxide, dihydroxyethyl laurylamine oxide, diisotridecyl lauroyl glutamate, dilauryl maleate / C20 olefin copolymer, dimaltosyl cyclodextrin, hydrogenated dimer dilinoleyl carbonate / dimethyl carbonate copolymer, polydimethylsiloxane crosspolymer, polydimethylsiloxane ethoxy glucoside, Dimethicone / lauryl dimethicone / bis-vinyl dimethicone crosspolymer, dimethicone / peg-15 crosspolymer, dimethyl decamide, dimethyl cocamide, dimethyl laurylamine isostearate, dioleyl phosphate, dipropylene glycol isobornyl ether, lauryl cetyl alcohol, bis-ethoxydiglycol cyclohexane 1,4-dicarboxylate, ethyl hydroxyethylcellulose, bis-ethyl ppg-behenate dimonium methosulfate, bis-(ethyl ppg-3behenate) dimonium methosulfate, ethylene vinyl acetate copolymer, ethylene / acrylic acid copolymer, ethylene / sodium acrylate copolymer, feruloyl soy glyceridesglyceride), perfluorocyclohexylmethanol, perfluoroheptane, perfluoromethylcyclohexane, perfluoromethyldecahydronaphthalene, gum ghatti, glucose pentaacetate, α-dextrose pentaacetate, glyceryl polyether-7 malate, glyceryl polyether-8 hydroxystearate, glyceryl polyether-7 benzoate, hydrogenated glyceryl abietate, glycol palmitostearate, acetylated glycol stearate, glycosyl trehalose, grape seed oil glyceryl polyether-8 ester, grape seed oil polyglyceryl-6 ester, maleated hexene / propylene copolymer, hydroxyquinoline sulfate, hydroxyapatite, hydroxybutyl methylcellulose, bis-hydroxyethoxypropyl dimethicone beeswax esters, bis-hydroxyethoxypropyl dimethicone isostearate, Hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, hydroxyethylcellulose, hydroxyethyl isostearyl isopropanolamine, hydroxypropylcellulose, hydroxypropyl guar gum, hydroxypropyl methylcellulose, hydroxypropyl xanthan gum, hydroxypropyltrimonium inulin, hydroxypropyltrimonium xanthan gum, hydroxystearic acid / linolenic acid / linoleic acid polyglyceryl esters, hydroxystearic acid / linolenic acid / oleic acid polyglyceryl esters, inulin lauryl carbamate, synthetic japan wax, jojoba glyceryl polyether-8 esters, hydrogenated lanolin alcohol, lanolinamide DEA, lauryl alcohol, lauryl diphosphate, lauryl dodecenylsuccinate, lauryl / myristyl wheat bran / straw glycosides wheat bran / straw glycoside), hydrogenated lime seed oil, magnesium alginate, maltitol laurate, maltodextrin, methoxy peg-22 / dodecyl glycol copolymer, methoxy peg / ppg-25 / 4 dimethicone, methylcellulose, methyl vinyl ether-maleic anhydride copolymer, montmorillonite, myristyl / palmitamide butylguanidine acetate, myristyl aminopropionate, myristyl alcohol, oleic / linoleic / linolenic polyglyceryl, olive oil alcohol, hydrogenated olive oil caprylyl ester, hydrogenated olive oil cetyl ester, hydrogenated olive oil decyl ester, hydrogenated olive oil hexyl ester, hydrogenated olive oil lauryl ester, hydrogenated olive oil myristyl ester, hydrogenated olive oil stearyl ester, hydrogenated orange seed oil, paraffin, palm kernel oil amide DEA, palm kernel oil amide MEA, palm kernel oil amide MIPA, palm oleic acid amide DEA (palmamideDEA), palmitoleic acid amide MEA, palmitoleic acid amide MIPA, arachidamide MEA, arachidamide MIPA, pectin, tri (peg-2 phenylalanyl carboxamido) cyclohexane, peg-2 tallow amide DEA, peg-4peg-12 polydimethylsiloxane, peg-5 pentaerythrityl dihydroxymethylpropionate-2 dendrimer, peg-5 pentaerythrityl dihydroxymethylpropionate-3 dendrimer, peg-5 pentaerythrityl dihydroxymethylpropionate-4 dendrimer, peg-7 propylheptyl ether, peg-7m, peg-8 polydimethylsiloxane / polysorbate 20 crosspolymer, peg-8 propylheptyl ether, peg-9m, peg-12 carnauba wax, peg-12 glyceryl linoleate, peg-14m, peg-20m, peg-23m, peg-65m, peg-90m, peg-100 / IPDI copolymer, peg-114 polylactic acid, peg-115m, peg-160m, peg-180m, peg-400, peg-45 / dodecyl glycol copolymer, peg-450, peg-500, peg / ppg- 10 / 3 oleyl ether dimethicone, peg / ppg-100 / 70 tocopheryl ether, bis-peg / ppg-15 / 5 dimethicone, peg / ppg-18 / 18 isostearate, peg / ppg-18 / 18 laurate, peg / ppg-2 / 5 tocopheryl ether, peg / ppg-20 / 23 dimethicone, bis-peg / ppg-20 / 5 peg / ppg-20 / 5 dimethicone, peg / ppg-2000 / 200 copolymer, peg / ppg-23 / 6 poly Dimethicone, peg / ppg-30 / 10 tocopherol ether, peg / ppg-5 / 10 tocopherol ether, peg / ppg-5 / 20 tocopherol ether, peg / ppg-5 / 30 tocopherol ether, peg / ppg-50 / 20 tocopherol ether, peg / ppg-6 / 4 polydimethylsiloxane, peg / ppg-70 / 30 tocopherol ether, peg / ppg-8 / 3 laurate, pentadecanol, microcrystalline petrolatum wax, phosphatidic acid, phosphatidylserine, phosphatidylglycerol, pineamidopropyl betaine betaine), Poly C10-30 Alkyl Acrylate, Polyacrylate Crosspolymer-4, Polyacrylate Crosspolymer-6, Polyacrylate Crosspolymer-11, Polyacrylate Crosspolymer-14, Polyacrylate-10, Polyacrylate-11, Polyacrylate-27, Polyacrylate-28, Polyacrylic Acid, Polyester-14, Polyester-15, Polyethylene / isopropyl maleate / MA copolyolcopolyol), polyglyceryl-2 diisostearate / isophorone diisocyanate copolymer, polyglyceryl-3 sunflower seed methyl ester / citrate crosspolymer, polyglyceryl-4 diisostearate / polyhydroxystearate / sebacate, polyglyceryl-6 behenate, polypropylene glycol, polytrimethylene terephthalate, polyquaternium crosspolymer-2, polyquaternium-65, polyquaternium-83, polyquaternium-102, polyquaternium-103, polysilicone-25, polyurethane-29, polyvinyl acetate, polyvinyl pyrrolidone, potassium alginate, potassium behenoyl hydrolyzed rice protein, potassium behenoyl hydroxyproline hydroxyproline), carbomer potassium, carrageenan potassium, stearoyl hydrolyzed rice protein potassium, undecylenyl alginate potassium, undecylenyl carrageenan potassium, undecylenyl hydrolyzed corn protein potassium, undecylenyl hydrolyzed soy protein potassium, undecylenyl hydrolyzed wheat protein potassium, hydrolyzed potato tuber extract, ppg-4 jojoba alcohol, ppg-4 laureth-2, ppg-4 laureth-5, ppg-6-laureth-3, ppg-20 tocopherol polyether-5, ppg-10 jojoba acid, ppg-2-butanol polyether-2, propyl ester of PVM / MA copolymer, unsaponifiable matter of sweet almond oil, surface pseudoyeast smut powder fungus / tea seed oil / glucose / wild soybean meal / malt extract / yeast extract fermentation product filtrate, PVP montmorillonite, PVP / decene copolymer, apple (pyrus malus fiber, quaternium-90 sepiolite, rhamnolipids, sclerotium gum, hydrogenated sesame seed oil, sesquiethoxytriethanolamine, sesquioctyldodecyl lauroyl glutamate, shea butter glycerides, silica dimethyl silylate, silica silylate, beta-sitosterol, sodium acrylate / acryloyldimethyltaurate / dimethylacrylamide crosspolymer, sodium acrylate / sodium acryloyldimethyltaurate copolymer, sodium acrylate / sodium acryloyldimethyltaurate / acrylamide copolymer, sodium acrylate / vinyl alcohol copolymer, sodium acrylate / vinyl isodecanoate crosspolymer, sodium acryloyldimethyltaurate / acrylamide / VP copolymer, sodium acryloyldimethyltaurate / VP crosspolymer, sodium arachidate, sodium C4-12 olefin / maleic acid copolymer, sodium carboxymethylcellulose, sodium carboxymethyl dextran, sodium carboxymethyl starch, sodium carrageenan, sodium cellulose sulfate conjugatebinding), sodium cocoyl barley amino acids, sodium cocoyl / stearoyl (alanine / arginine / asparagine / aspartic acid / glutamic acid / glutamine / glycine / histidine), sodium cyclodextrin sulfate, sodium dextrin octenylsuccinate, sodium lanolin polyether sulfate, sodium polyacrylate, sodium polyacrylate starch, sodium polyacryloyldimethyl taurate, sodium poly-gamma-glutamate, sodium poly-gamma-glutamate crosspolymer, sodium polyglutamate crosspolymer, sodium polymethacrylate, sodium polynaphthalenesulfonate, sodium polystyrenesulfonate, sodium octenylsuccinate starch, sodium styrene / MA copolymer copolymer), sodium tocopheryl phosphate antioxidant, sodium trehalose octenylsuccinate, sodium / TEA-undecylenoyl alginate, sodium / TEA-undecylenoyl carrageenan, sorbitan palmitate, soy protein phthalate, soyamide DEA, hydrangea extract, starch hydroxypropyltrimonium chloride, isosteareth-200 palmitate, stearic acid, stearyl alcohol, stearyl glycol, stearyl vinyl ether / MA copolymer, sterculia spinulosa gum, stigmasteryl chloride, stigmasteryl nonanoate, stigmasteryl succinate, styrene / MA copolymer, sucrose polypalmitate, sunflower oil ethyl ferulate, sunflower oil polyglyceryl-10 esters, sunflower oil polyglyceryl-6 esters, tallow alcohol alcohol), tallow amide beauty agent, soy bean gum, triethanolamine alginate (TEA-alginate), TEA-dextrin octenylsuccinate TEA salt (TEA-dextrin octenylsuccinate), myristyl eicosanoic acid, myristyl octadecanoic acid, myristyl octadecyl behenate, myristyl octadecyl myristate, myristyl octadecyl stearate, tetrasodium etidronic acid, theobroma cacao seed butter glyceride, tocopheryl succinate methyl glucamide (tocopheryl succinatemethylglucamide), Tremella polysaccharide, triaconten / VP copolymer, 1-tridecanol, tripropylene glycol, undeceth-40, undecylenyl inulin, undecylenyl xanthan gum, vinyl alcohol / vinyl formamide copolymer, bis-vinyl dimethicone / dimethicone copolymer, bis-vinyl dimethicone / peg-10 dimethicone crosspolymer, hydrogenated microcrystalline wax, wellan gum, xanthan gum, zinc undecylenyl hydrolyzed wheat protein.

[0145] The cosmetic composition according to the invention may also contain microbial ingredients (e.g. filtrates, extracts and / or ferments of yeasts (Saccharomyces), lactobacilli (Lactobacillus) and / or microbial-based preparations such as Lallemand's P&R, Lallbiome TM 300. Lallbiome TM 201, Hydra and / or Zn.

[0146] The cosmetic composition according to the present invention may also contain cross-linked hyaluronic acid. For example, this compound can be used as a thickener to prepare a transparent moisturizing hydrogel. Specific examples include Hyacross TM Hyaluronic acid microspheres TG300 (sodium hyaluronate cross-linked polymer, Bloomage Biotechnology Corp. Ltd.) and Hylasome TM EG10 (sodium hyaluronate crosspolymer, Vantage Personal Care), but not limited thereto.

[0147] Methods of manufacturing and replacing microplastics

[0148] Another aspect of the invention relates to a method for making a cosmetic composition as defined herein (e.g. a sunscreen, skin care and / or hair care composition). A related aspect relates to the use of a biopolymer as described herein for replacing microplastics in a cosmetic composition.

[0149] In one embodiment, the method comprises replacing at least a portion of the microplastics that may be present in the cosmetic composition with a biopolymer as defined herein (e.g., a biopolymer suspension). In an embodiment, the biopolymer suspension comprises a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent.

[0150] In one embodiment, a method of making a sunscreen composition and / or a method of replacing microplastics in a sunscreen composition comprises: (1) providing a biopolymer suspension as defined herein (e.g., a suspension of nanosized insoluble and / or semi-soluble particles stably dispersed in a polar solvent); and (2) mixing the biopolymer suspension with at least one of a sensory enhancer, a film former, an emulsifier, an emollient, a UVB filter, a UVA filter, and a broad-spectrum filter.

[0151] In one embodiment, a method of making a skin care composition and / or a method of replacing microplastics in a skin care composition comprises: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; (2) mixing the biopolymer suspension with at least one of a moisturizer, an emollient, an occlusive, a ceramide, an emulsifier, a thickener, a preservative, an antioxidant, a fragrance, and a functional ingredient.

[0152] In one embodiment, a method for making a hair care composition and / or a method for replacing microplastics in a hair care composition comprises: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; (2) mixing the biopolymer suspension with at least one of a surfactant, a thickener, an opacifying agent, a ceramide, a conditioning agent, a pH buffer, a chelating agent, a preservative, an emulsifier, a stabilizer, an antioxidant, a fragrance, and a functional ingredient.

[0153] In an embodiment, a method of making a cosmetic composition and / or a method of replacing microplastics in a cosmetic composition comprises adding ingredients in sequence. The method of making may also include combining water-based ingredients together, combining oil-based ingredients together, and mixing the two phases together. Water may be added to the oil, or the oil may be added to the water. An emulsifier (if any) may be in either phase. Additional water or oil may also be added during the mixing process. Of course, there may be a variety of variations depending on the cosmetic product to be manufactured, the desired properties of the final cosmetic product, etc. It is within the skill of those skilled in the art to test and determine suitable manufacturing processes and steps.

[0154] Those skilled in the art may recognize or be able to determine many equivalents to the specific procedures, embodiments, claims and examples described herein using only routine experimentation. Such equivalents are considered to be within the scope of the present invention and are covered by the appended claims. The present invention is further illustrated by the following examples, which should not be construed as further or specific limitations.

[0155] Example

[0156] Embodiment 1: Mineral filter sunscreen

[0157] Tables 2 and 3 provide non-limiting examples of contemplated sunscreen compositions.

[0158] Table 2 : Mineral filter sunscreen

[0159] Element# Brand Name Amount(%w / w) 1 water 31.90 2 Xanthan gum 0.50 3 Glycerin USP 2.50 4 <![CDATA[Biopure TM GLDA]]> 0.10 5 <![CDATA[Dermofeel TM NC]]> 5.00 6 <![CDATA[Eumulgin TM SG]]> 1.00 7 <![CDATA[Polyaquol TM VO4]]> 0.10 8 <![CDATA[UV-CUT ZNO TM 65CC]]> 38.00 9 <![CDATA[Kester TM K60 P]]> 1.00 10 <![CDATA[Lexfilm TM SUN]]> 5.00 11 <![CDATA[Dub Zenoat TM ]]> 3.00 12 Vitamin E 0.50 13 <![CDATA[Geogard TM ECT]]> 1.00 14 <![CDATA[Sepimat P TM ]]> 2.00 15 Biopolymer (Chitosan) 5.00 16 Citric acid solution (50% w / w) 0.40 17 water 2.00

[0160] Table 3 : Chemical filter sunscreen

[0161] Element# Brand Name Amount(%w / w) 1 water 48.90 2 Glycerin USP 2.50 3 Xanthan gum 0.50 4 <![CDATA[Biopure TM GLDA]]> 0.10 5 <![CDATA[Eusolex TM OCR Octocrylene]]> 3.00 6 <![CDATA[Parsol TM EHS Octisalate]]> 5.00 7 <![CDATA[Finsolv TM TPP]]> 2.00 8 <![CDATA[Lexfilm TM SUN]]> 5.00 9 <![CDATA[Eusolex TM 9020Avobenzone]]> 3.00 10 <![CDATA[Eusolex TM HMS Homosalate]]> 10.00 11 <![CDATA[DUB Zenoat TM ]]> 2.50 12 <![CDATA[Dermofeel TM NC]]> 5.00 13 <![CDATA[Eumulgin TM SG]]> 1.00 14 Vitamin E 0.50 15 <![CDATA[Geogard TM ECT]]> 1.00 16 <![CDATA[Sepimat P TM ]]> 2.00 17 Biopolymer (Chitosan) 5.00 18 water 3.00

[0162] In these compositions, the biopolymer composition is a chitosan suspension. Such a composition can be prepared as follows. The chitosan suspension is prepared by a 1.5 L Supermill Plus with 1.25 mm zirconium oxide beads. TM Chitosan suspensions were prepared by milling in 20 L batches, with typical milling conditions being a rotation speed of 2400 FPM (feet per minute) and a pump flow of 8 GPH (gallons per hour), using 956 mL of 1.25 mm zirconium oxide beads, wherein 20 liters of slurry were processed at 10% solids content (2.2:20), and the particles of the chitosan powder had a D99 of 19 μm, a D50 of 5 μm, and a D10 of 2 μm.

[0163] The manufacturing process contemplated for these sunscreen compositions is as follows.

[0164] Phase A

[0165] Step 1- Place ingredient #1 in a master jar.

[0166] Step 2 - Place ingredients #2 and #3 in a stainless steel container and stir until mixture is smooth. Add mixture to main pot and stir until smooth.

[0167] Step 3 - While stirring, add ingredient #4 to the main pot. Stir until the mixture is homogeneous. Heat to 75°C ± 2°C. Once temperature is reached, stop heating and maintain temperature. Stir until the mixture is homogeneous.

[0168] Phase B

[0169] Step 4 - Place ingredients #5, #6, #7, #8, #9, #10, #11 and #12 in a stainless steel container. Heat to 75°C ± 2°C. Once temperature is reached, stop heating and maintain temperature. Stir until mixture is homogeneous.

[0170] Phase A+B

[0171] Step 5 - While stirring, add the mixture from step 4 to the main tank. Stir until the mixture is homogeneous. Start cooling and keep stirring until the temperature reaches 40℃ ± 2℃.

[0172] Phase A+B+C

[0173] Step 6 - When the temperature in the main tank is below 40°C ± 2°C, add ingredients #13, #14, #15, #16, and #17 one by one. Stir between each addition until the mixture is homogeneous. Keep stirring until the temperature is 25°C ± 2°C.

[0174] Embodiment 2: SPF 30 sunscreen products

[0175] Table 4 provides non-limiting examples of contemplated sunscreen compositions providing SPF 30 protection.

[0176] Table 4 : SPF 30 products

[0177]

[0178] The manufacturing process envisioned for this sunscreen composition is as follows. Wet ball mill B in A until thickened and homogenized. Grind C in AB using a rotor / stator mill. Switch to sweep mixing and add D to ABC.

[0179] Embodiment 3: Zinc Oxide (SPF 30+) Water-in-Oil Emulsion

[0180] Table 5 provides non-limiting examples of contemplated sunscreen compositions comprising zinc oxide and providing SPF 30+ protection.

[0181] Table 5 : Zinc Oxide SPF 30+ Products

[0182]

[0183]

[0184] The manufacturing process envisioned for this sunscreen composition is as follows. Wet ball mill A and add B. Heat AB to 70°C. Grind D in C using a rotor stator mill at 10K rpm for 5 minutes, heat CD to 70°C. Grind CD in ABC using a rotor stator mill at 5K rpm for 5 minutes. Cool the batch using side sweep mixing and add F at 45°C. Cool to 30°C.

[0185] Embodiment 4: Rinse-Out Cream Conditioner

[0186] Table 6 provides non-limiting examples of contemplated rinse-off cream conditioners.

[0187] Table 6 : Rinse-out cream conditioner

[0188]

[0189] The manufacturing process envisioned for this sunscreen composition is as follows. Wet ball mill A. Heat A and B to 80°C. Homogenize B in A using a rotor stator mill at 5K rpm for 5 minutes. Propeller mix AB and cool. Add C at 45°C. Cool to 30°C.

[0190] Embodiment 5: Spray Conditioner

[0191] Table 7 provides non-limiting examples of contemplated rinse-off cream conditioners.

[0192] Table 7 : Spray conditioner

[0193]

[0194] The manufacturing process contemplated for this sunscreen composition is as follows. Wet ball mill A. Propeller mix B into A. Propeller mix C into AB.

[0195] Embodiment 6: Conditioning Shampoo

[0196] Table 8 provides non-limiting examples of contemplated conditioning shampoos.

[0197] Table 8 : Conditioning Shampoo

[0198]

[0199] The manufacturing process contemplated for this sunscreen composition is as follows. Wet ball mill A. Sweep mix B into A. Add C. Add D. Add E.

[0200] Embodiment 7: Reduces wrinkles and fine lines - Cellulose / chitosan based composition

[0201] In this study, the aim was to evaluate the effect of using a cellulose / chitosan based composition according to the invention comprising 5% w / w mushroom chitosan, 0.4% w / w cellulose, 5% w / w Dermosoft TMand water [Batch DEF23041801]. Briefly, chitosan and cellulose were wet ground with water for 30 minutes (stirrer pressure 7-9 PSI; stirrer speed 3000 rpm). Dermosoft was added at 30 minutes. TM preservatives and continue grinding for another 60 minutes. The temperature of the mixture is maintained between about 30°C and 40°C.

[0202] The above cellulose / chitosan based compositions were evaluated by an independent third party laboratory using standard guidelines. Briefly, 1.5 g of the composition was applied twice daily (morning and evening) to the volar surface of the face of 12 volunteers for 10 days. Measurements and photographs were taken before application of the product (T0), 15 minutes after the first application (T15Min), and after 10 days of use of the product (T10 days ± 2 days). 3D images were obtained by VisioTOP- +AEVA-V4 software to monitor the effect of the product. The skin sites analyzed at different times were set on random sides of the face for each analysis. Using VisioTOP- This area is evaluated in each macro photo. The instrument also measures the most representative parameters for anti-wrinkle efficacy evaluation, namely Ra and Rz, where Ra is the linear average profile roughness and Rz is the average maximum profile height difference.

[0203] The results were very positive, with improvements in crow's feet roughness and wrinkles. In fact, the study observed a statistically significant reduction in wrinkles after application of the cellulose / chitosan-based composition tested. The average reduction was 4.1% after just 15 minutes and 5.7% after 10 days.

[0204] In addition, the cellulose / chitosan-based composition can also be used to "smooth" the skin. 15 minutes after the application of the product, it was shown that the mean Ra parameter (linear mean profile roughness) decreased by a mean value equal to -2.2%. After 10 days of use, the mean value was equal to -3.0%. The decrease at these two time points was considered statistically significant compared to the mean values ​​measured before application.

[0205] Therefore, these results clearly demonstrate that the biopolymer composition according to the present invention is active and effective in reducing both wrinkles and fine lines.

[0206] Example 8: Cellulose Waterproofing Test

[0207] The waterproofing effect of the cellulose biopolymer composition according to the present invention was evaluated by an independent third-party laboratory. The composition was prepared by ball milling and contained 5% w / w cellulose and water [Batch: CMC B2AC, 450G]. Briefly, a 1.5L Supermill PLUS TM Cellulose was pre-grinded in isopropanol in a horizontal mill for 23 hours (grinding speed 2750 FPM, 1.25 mm zirconium oxide beads). TM The product was wet ground in a horizontal mill using 1.25 mm zirconium oxide beads at a flow rate of 11 gallons per hour, a grinding speed of 2400 FPM, and a PSI of 3.

[0208] Briefly, the composition was applied to the forearm area of ​​14 volunteers and then evaluated by a professional technician for its water resistance after spraying with water. During the evaluation, the technician gave a score of 1 to 10 on the durability of the composition after 10 sprays of water from the initial application.

[0209] Typically, the tested product is considered waterproof if the average of these scores is greater than or equal to 7 after 10 sprays of water from the application of the product. In this case, the average score for the durability of the composition is 7.6 out of 10. Therefore, this objective evaluation by professionals in a third-party laboratory shows that the cosmetic biopolymer composition according to the invention has a waterproof effect.

[0210] Embodiment 9: Cellulose patch test

[0211] The test was conducted by an independent third party laboratory to evaluate the topical skin tolerance of the cellulose biopolymer composition according to the present invention. The composition was from the same batch as Example 8 (i.e., prepared by ball milling, comprising 5% w / w cellulose and water [Batch: CMC B2AC, 450G].

[0212] In brief, about 0.07-0.1 ml of the test product (i.e., the cellulose biopolymer composition) was applied to the intact skin of the back of adult volunteers. The product remained in contact with the skin for 48 hours under a semi-occluded patch. In addition to the test product, a "negative" control blank patch was also applied. The effects caused by applying the test product were observed 15 minutes and 24 hours after the patch was removed to evaluate skin reactions, considering parameters such as erythema, desquamation, edema, and blisters, with severity scores ranging from 0 to 3.

[0213] The mean irritation index (MII) of the test product was 0 at both time periods (15 minutes and 24 hours after patch removal). Therefore, the test product was classified as "non-irritating" and "dermatologically tested as skin compatible" according to the reference standard (data not shown). Therefore, these results confirm that the cosmetic biopolymer composition according to the present invention is dermatologically safe.

[0214] Embodiment 10: Evaluation of skin barrier effect and water resistance - cellulose / chitosan based compositions

[0215] In this study, the aim was to demonstrate the water repellent effect of a cellulose / chitosan based composition according to the invention. A cellulose / chitosan based composition was prepared by wet grinding according to the details provided in Example 7 (i.e. 5% w / w mushroom chitosan, 0.4% w / w cellulose, 5% w / w Dermosoft TM and water) [Batch DEF23041801].

[0216] The evaluation involved thirteen (13) volunteers and two professional technicians. To evaluate the waterproofing effect, a The cellulose-based composition under investigation was compared with a baseline pigment of Terra Brown. The evaluation process consisted of spraying water on the forearm area of ​​the volunteers after application of the tested product (i.e. the cellulose / chitosan-based composition as defined above). During the evaluation, two technicians assigned a score ranging from 1 to 10 according to the durability of the tested product after 10 water sprays. If the average of these scores was equal to or greater than 7 after 10 water sprays from the initial application, the product was considered to be waterproof.

[0217] Two professional technicians gave the tested products an average rating of 9.1 out of 10 for durability. This high rating indicates a high level of water repellency. Thus, the biopolymer composition according to the invention can withstand 10 water sprays after application.

[0218] The study further concluded that the products tested resulted in "a significant reduction in the transepidermal water loss (TEWL) value of the skin to which they were applied". These results further confirm that the biopolymer composition according to the invention has an important skin barrier effect.

[0219] Embodiment 11: Cellulose Challenge Test

[0220] "Challenge testing" is a useful method for evaluating the efficacy of preservative systems in non-sterile cosmetics, pharmaceuticals and similar products. This testing involves artificially contaminating the product in the laboratory to simulate microbial contamination that the product may encounter during manufacturing, storage and consumer use. This testing provides critical information about the product's ability to resist microbial attack and maintain stability. Since cosmetics and similar products are not required to be sterile, they may be subject to a certain level of environmental microbial contamination, which needs to be controlled through appropriate preservation. Challenge testing goes beyond ordinary environmental contamination by introducing high concentrations of microorganisms and can evaluate the reduction in microbial growth at different time points. This testing follows the guidelines outlined in the United States Pharmacopeia and is performed before the product is checked for total microbial load.

[0221] The inoculum of this challenge test is composed of various microbial strains at different concentrations. The strains used include Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231 and Aspergillus brasiliensis ATCC 16404.

[0222] This challenge test was performed with a cellulose based composition according to the invention according to method USP 61. The composition was prepared by ball milling and comprised 5% w / w cellulose and water [Batch: CMC B2AC, 450G].

[0223] The different microbial strains were suspended in physiological solution and all strains were cultured at 10 5 Up to 10 6 The final concentration in the range between 1.5 and 2.5 was inoculated in the tested product. The treated samples were then stored at room temperature in the dark until microscopic slides were prepared for microbial enumeration. The results are shown in Table 9 below.

[0224] Table 9: Challenge testing of cellulose-based compositions of the invention

[0225]

[0226] The results of the study were very positive, as the tested compositions effectively resulted in a microbial reduction (>2 log) for all stains at 14 days. The tested compositions were also effective in preventing an increase in growth of all tested strains, as measured at 28 days. Therefore, the sponsors of the study concluded that the cellulose-based compositions according to the present invention fully meet the requirements for preservative efficacy testing for topical products, according to USP regulations.

[0227] Embodiment 12: Successful corrosion challenge testing

[0228] USP 51 preservative challenge was performed using a chitosan based composition according to the present invention [Batch CMCB3.1], which further comprised a commercially available preservative system Spectrastat TM (Univar Solutions).

[0229] Use basket mill BM-100 TM Chitosan was pre-milled in isopropanol for 2.5 hours (mixing speed 15 Hz, milling speed 40 / 1600 Hz, zirconia beads 1.4 to 1.7 mm). TM The resulting product was wet ground in a horizontal mill. Six (6) passes were performed using 1.25 mm zirconium oxide beads at a flow rate of 5.5 gallons per hour, a grinding speed of 2400 FPM, and a PSI of 1-2. Next, the Spectrastat TM Add to the ground product and mix for a few minutes according to the supplier's recommendations.

[0230] The test was conducted by a third-party laboratory accredited by Health Canada. In short, the test was conducted by exposing the composition to the following pathogens according to the USP 51 test method: Aspergillus brasiliensis ATCC 16404, Candida albicans ATCC10231, Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027, and Staphylococcus aureus ATCC 6538. The composition was checked at three different time intervals (day 0, day 14, day 28) to determine the growth of pathogens. Interestingly, no significant growth of bacteria was found from day 14 to day 28, and the sponsors of the study concluded that "the submitted preparation can be properly preserved."

[0231] These results demonstrate that cosmetic biopolymer compositions according to the present invention can be successfully preserved and that such compositions can prevent pathogen growth, particularly when the compositions contain a cosmetically acceptable preservative such as Spectrastat TM hour.

[0232] Embodiment 13: Evaluation of skin barrier effect and water resistance - cellulose / chitosan / beeswax based compositions

[0233] In this study, the aim was to demonstrate the water-repellent effect of a cellulose / chitosan / beeswax based composition according to the invention. The cellulose / chitosan / beeswax based composition comprised 5.7% w / w mushroom chitosan, 0.43% w / w alpha cellulose, 5% w / w Dermasoft TM , 0.55% cetyl alcohol and 0.93% beeswax [Batch DEF23051502].

[0234] The composition was prepared as follows. First, an original batch of "milled formulation" comprising alpha cellulose, chitosan and water was milled in a NETZSCH LabStar Zeta Small Media Mill. TM Grind for 110 minutes at 57°C (PSI 6.9-11, stirrer speed 3000 rpm, chiller on to maintain 32°C temperature). Separately, cetyl alcohol, beeswax synthetic pellets and Dermosoft TM Melt together to make the following "Oil Mixture" mixture. Mix the Oil Mix until the temperature drops to 52°C. Add the Milled Formulation (385 grams total) to the Oil Mix 1 / 6 at a time. Mix the two together until the temperature drops to 45°C. Once uniformly mixed, add this resulting mixture very slowly to the ball mill, adding the remainder of the original batch of Milled Formulation at the 110 minute mark. Then continue grinding for another 10 minutes.

[0235] The evaluation involved thirteen (13) volunteers and two professional technicians. The evaluation process was carried out according to Example 10 above.

[0236] In this test, two professional technicians gave the tested products an average rating of 9.7 out of 10 for their durability. This very high rating indicates a high level of water repellency. Thus, the biopolymer composition according to the invention can withstand 10 water sprays after application.

[0237] The study further concluded that the products tested resulted in "a significant reduction in the transepidermal water loss (TEWL) value of the skin to which they were applied". These results further confirm that the biopolymer composition according to the invention has an important skin barrier effect.

[0238] Embodiment 14: Cellulose / Chitosan Patch Test

[0239] The test was conducted by an independent third-party laboratory to evaluate the topical skin tolerance of the cellulose biopolymer composition according to the present invention. The composition was from the same batch as Example 7 (i.e. 5% w / w mushroom chitosan, 0.4% w / w cellulose, 5% w / w Dermosoft TM The test procedure was the same as the cellulose patch test of Example 9.

[0240] In this test, the mean irritation index (MII) of the test product was 0 at both time periods (15 minutes and 24 hours after patch removal). Therefore, the test product was classified as "non-irritating" and "dermatologically tested as skin compatible" according to the reference standard (data not shown). These results further confirm that the cosmetic biopolymer composition according to the present invention is dermatologically safe.

[0241] Embodiment 15: Reduces wrinkles and fine lines - Cellulose / Chitosan / Beeswax based composition

[0242] In this study, the aim was to evaluate the effect of using a cellulose / chitosan / beeswax based composition according to the invention to reduce wrinkles and fine lines. The composition comprised 5.7% w / w mushroom chitosan, 0.43% w / w alpha cellulose, 5% w / w Dermasoft TM , 0.55% cetyl alcohol and 0.93% beeswax, prepared according to the above Example 13 [Batch DEF23051502].

[0243] Using the same evaluation process as described in Example 7, the efficacy of the above composition was evaluated on 13 healthy male and female volunteers by an independent third party laboratory.

[0244] The results were very positive, with improvements in crow's feet roughness and wrinkles. In fact, the study observed a statistically significant reduction in wrinkles after application of the cellulose / chitosan-based composition tested. An average reduction of 5.9% was seen after only 15 minutes.

[0245] In addition, the composition can also be used to "smooth" the skin. 15 minutes after the application of the product, it showed a reduction in the mean Ra parameter (linear mean profile roughness) equal to a mean value of -4.5%. After 10 days of use, the mean value was equal to -2.2%. The reduction at the 15-minute time point was considered statistically significant compared to the mean value measured before application.

[0246] Therefore, these results clearly demonstrate that the biopolymer composition according to the present invention is active and effective in reducing both wrinkles and fine lines.

[0247] ***

[0248] Headings are included herein for reference and to help locate certain sections. These headings are not intended to limit the scope of the concepts described therein, and these concepts may have applicability throughout other parts of the specification. Therefore, the present invention is not intended to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

[0249] The singular forms "a", "an", and "the" include corresponding plural references unless the context clearly dictates otherwise. Thus, for example, reference to "a biopolymer" includes one or more such biopolymers, and reference to "a method" includes reference to equivalent steps and methods known to those of ordinary skill in the art, which steps and methods may be modified or substituted for the methods described herein.

[0250] Unless otherwise stated, all numerical values ​​of the amount of the expression composition, reaction conditions, concentration, property, etc. used in the specification and claims should be understood to be modified by the term "about" in all cases. At least, each numerical parameter should be interpreted at least according to the reported significant digits and using conventional rounding techniques. Therefore, unless otherwise stated, the numerical parameters described in this specification and the appended claims are approximate values, which may change according to the properties sought to be obtained. Although the wide range of numerical ranges and parameters stating the embodiments are approximate values, the numerical values ​​described in the specific examples are reported as accurately as possible. However, any numerical value inherently contains certain errors caused by the changes in experiments, test measurements, statistical analysis, etc.

[0251] It should be understood that the examples and embodiments described herein are for illustrative purposes only and that various modifications or changes thereto may be suggested to those skilled in the art, which are included within the scope of the present invention and the appended claims.

Claims

1. A cosmetic composition comprising biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension, wherein the stable homogeneous aqueous suspension comprises insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent.

2. The cosmetic composition according to claim 1, wherein The cosmetic composition consists of a sunscreen composition, which further comprises at least one of a sensory enhancer, a film former, an emulsifier, a co-emulsifier, an emollient, a UVB filter, a UVA filter, and a broad-spectrum filter.

3. The cosmetic composition according to claim 1, wherein The cosmetic composition consists of a skin care composition, which further comprises at least one of a moisturizer, an emollient, an occlusive agent, a ceramide, an emulsifier, a thickener, a preservative, an antioxidant, a fragrance, and a functional ingredient.

4. The cosmetic composition according to claim 1, wherein The cosmetic composition consists of a hair care composition, which further comprises at least one of a surfactant, a thickener, an opacifier, a ceramide, a conditioning agent, a pH buffer, a chelating agent, a preservative, an emulsifier, a stabilizer, an antioxidant, a fragrance, and a functional ingredient.

5. A sunscreen composition comprising: a biopolymer, and at least one of a sun protection factor (SPF) booster, a sensory enhancer, a film former, an emulsifier, a co-emulsifier, an emollient, a UVB filter, a UVA filter, and a broad spectrum filter.

6. The sunscreen composition according to claim 5, wherein The sunscreen composition comprises water, a biopolymer, a UVB filter, a UVA filter, an emollient and a preservative.

7. The sunscreen composition according to claim 6, further comprising one or more of an SPF booster, a moisturizer, an emulsifier and a film former.

8. The sunscreen composition according to any one of claims 5 to 7, wherein The composition comprises biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension.

9. The sunscreen composition according to any one of claims 5 to 8, wherein The composition comprises a homogenous aqueous suspension of insoluble and / or semi-soluble biopolymer particles.

10. The sunscreen composition according to claim 9, wherein The stable homogeneous aqueous suspension comprises insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent.

11. The sunscreen composition according to any one of claims 5 to 10, wherein The biopolymer consists of a stable homogeneous aqueous suspension comprising insoluble and / or semi-soluble biopolymer particles.

12. The sunscreen composition according to any one of claims 5 to 11, wherein The biopolymer is selected from the group consisting of chitin, chitosan, cellulose, hemicellulose, lignin, amylose, actin, fibrin, collagen, silk, fibroin, keratin, wool, alginic acid and mixtures thereof.

13. The sunscreen composition according to any one of claims 5 to 11, wherein The biopolymer is selected from gelatin, pectin, starch, pullulan, agarose, alginic acid, alginate, hyaluronic acid, RNA, DNA, xanthan gum, guar gum, latex, polymannos, suberin, keratin, horny carbon and mixtures thereof.

14. The sunscreen composition according to any one of claims 5 to 13, wherein The SPF booster is selected from acrylates / methacryloyloxyethyl phosphate copolymer, acrylates / methacryloyloxyethyl phosphate copolymer, methylcellulose, trimethoxybenzylidene pentanedione, styrene acrylate copolymer, microcrystalline cellulose, argan oil (and) tocopheryl acetate (and) bisabolol, arabinogalactan, butyloctyl salicylate, bis-hydroxyethoxypropyl dimethicone, C20-40 alcohol, diethylhexyl 2,6-naphthalene dicarboxylate, caprylyl glycol, butyloctyl salicylate, tridecyl salicylate (Alzo Inc.), pongamia seed oil, pongamia extract, ethylhexyl methoxypolyacrylonitrile, acrylates copolymer, poly C10-30 alkyl acrylate, polyamide 3, hydrogenated polycyclopentadiene (and) polyethylene (and) carnauba (carnauba) wax (and) tocopherol, polyurethane 34, hydrolyzed wheat protein / PVP crosspolymer and mixtures thereof.

15. The sunscreen composition according to any one of claims 5 to 14, wherein The sensory enhancer is selected from the group consisting of cyclopentasiloxane, cyclohexasiloxane, cyclopentasiloxane, disiloxane, trisiloxane, dimethicone, caprylyl methicone, phenyl trimethicone, cetyl dimethicone, bis-hydroxyethoxypropyl dimethicone, dimethicone / vinyl dimethicone crosspolymer, dimethicone / dimethicone crosspolymer, PEG-20 sorbitan cocoate, phenoxyethanol, ethylhexylglycerin, potassium sorbate, phenoxyethanol, methylparaben, isopropylparaben, isobutylparaben, butylparaben, and mixtures thereof.

16. The sunscreen composition according to any one of claims 5 to 15, wherein The film former is selected from the group consisting of cetyl dimethicone, stearyl dimethicone, phenyl trimethicone, bis-hydroxyethoxypropyl dimethicone, isododecane, polypropyl silsesquioxane, dimethicone / vinyl dimethicone crosspolymer, C30-45 alkyl methicone, C30-45 olefin, beeswax, candelilla (euphorbia cera) wax, carnauba (carnauba) wax, isododecane, acrylates polytrimethylsiloxymethacrylate copolymer, hydrogenated polycyclopentadiene, arabinogalactan, dihydroabietate methyl, trimethylsiloxysilicate, trimethylsiloxysilicate, polypropyl silsesquioxane, C30-45 alkyl dimethylsilyl polypropyl silsesquioxane, ethylenediamine / hydrogenated dimer linoleate copolymer bis-di-C14-18 alkylamide, acrylate copolymers, and mixtures thereof.

17. The sunscreen composition according to any one of claims 5 to 16, wherein The emulsifier is selected from acrylates / C10-30 alkyl acrylate crosspolymer, potassium cetyl phosphate, sodium cetearyl sulfate, C20-22 alkyl phosphate, PEG-30 dipolyhydroxystearate, polyglyceryl-2-dipolyhydroxystearate, polyglyceryl-3-diisostearate and a mixture thereof.

18. The sunscreen composition according to any one of claims 5 to 17, wherein The auxiliary emulsifier is selected from cetyl alcohol, stearyl alcohol, stearic acid, glyceryl stearate, sorbitan stearate, polyglyceryl 3 distearate and a mixture thereof.

19. The sunscreen composition according to any one of claims 5 to 18, wherein The emollient is selected from C12-15 alkyl benzoate, trisiloxane, squalene, butyloctanol salicylate, isopropyl isostearate, isostearyl isostearate, octyl carbonate, isopropyl myristate, dibutyl adipate, caprylic / capric triglyceride, coconut caprylate, dicaprylyl carbonate, ethylhexyl methoxypolyacrylonitrile, diethylhexyl 2,6-naphthalene dicarboxylate, butyloctanol salicylate, tridecyl salicylate, polyester-8, diethylhexyl eugenylmalonate, benzotriazolyl dodecyl p-cresol, propylheptyl octanoate and mixtures thereof.

20. The sunscreen composition according to any one of claims 5 to 19, wherein The UVB filter is selected from Uvivul TM T 150 (Ethylhexyl Triazone), Titanium Dioxide, Benzophenone-3 (Oxybenzone), Octyl Methoxycinnamate (Ethylhexyl Methoxycinnamate), Octocrylene, Ethylhexyl Salicylate (Octyl Salicylate), Homosalate (Homomanthyl Salicylate), Ensoril (Phenylbenzimidazole Sulfonic Acid), Amiloride (Isoamyl p-Methoxycinnamate), Polysilicone-15, Diethylhexyl Butamido Triazone (Isopropyl Alcohol), 4-Methylbenzylidene Camphor (Enzacamphor), and mixtures thereof.

21. The sunscreen composition according to any one of claims 5 to 20, wherein The UVA filter is selected from butyl methoxydibenzoylmethane, butyl methoxydibenzoylmethane (avobenzone), diethylamino hydroxybenzoyl hexyl benzoate (Univul TM a plus), terephthalylidene dicamphor sulfonic acid (Mexoryl TM SX), disodium phenyl dibenzimidazole tetrasulfonate (Neo Heliopan TM ap), Tinosorb TM S (Bis-ethylhexyloxyphenol methoxyphenyl triazine), Tinosorb TM M (methylene bis-benzotriazolyl tetramethylbutylphenol (and) water (and) decyl glucoside (and) propylene glycol (and) xanthan gum), Tinosorb TM A2B (tris-biphenyl triazine (and) water (and) decyl glucoside (and) butylene glycol (and) disodium phosphate (and) xanthan gum) and mixtures thereof.

22. The sunscreen composition according to any one of claims 5 to 21, wherein The broad-spectrum filter is selected from bis-ethylhexyloxyphenol methoxyphenyl triazine, tris-biphenyl triazine (nano), methylene bis-benzotriazolyl tetramethylbutylphenol, drometrizole trisiloxane, zinc oxide and mixtures thereof.

23. The sunscreen composition according to any one of claims 5 to 22, wherein The preservative is selected from benzyl alcohol, sodium benzoate, caprylyl glycol, caprylic glyceride, phenoxyethanol, hexylene glycol, hydroxyacetophenone, sodium dehydroacetate, sodium phytate and a mixture thereof.

24. The sunscreen composition according to any one of claims 5 to 23, wherein The sunscreen composition is formulated as a paste, ointment, cream, lotion, gel and / or emulsion.

25. The sunscreen composition according to any one of claims 5 to 24, wherein The sunscreen composition provides a sun protection factor (SPF) of at least 15, or at least 30, or at least 45, at least 50, or at least 60.

26. Use of a sunscreen composition as defined in any one of claims 5 to 25 for protecting the skin from sunlight.

27. A method of skin protection which comprises applying to the skin a sunscreen composition as defined in any one of claims 5 to 25.

28. A skin care composition comprising: a biopolymer, and at least one of a humectant, an emollient, an occlusive agent, a ceramide, an emulsifier, a thickener, a preservative, an antioxidant, a fragrance, and a functional ingredient.

29. The skin care composition according to claim 28, wherein The composition comprises biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension.

30. A skin care composition according to any one of claims 28 or 29, wherein The composition comprises a homogenous aqueous suspension of insoluble and / or semi-soluble biopolymer particles.

31. The skin care composition according to claim 30, wherein The stable homogeneous aqueous suspension comprises insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent.

32. A skin care composition according to any one of claims 28 to 31, wherein The biopolymer consists of a stable homogeneous aqueous suspension comprising insoluble and / or semi-soluble biopolymer particles.

33. A skin care composition according to any one of claims 28 to 32, wherein The biopolymer is selected from the group consisting of chitin, chitosan, cellulose, hemicellulose, lignin, amylose, actin, fibrin, collagen, silk, fibroin, keratin, wool, alginic acid and mixtures thereof.

34. A skin care composition according to any one of claims 28 to 32, wherein The biopolymer is selected from gelatin, pectin, starch, pullulan, agarose, alginic acid, alginate, hyaluronic acid, RNA, DNA, xanthan gum, guar gum, latex, polymannos, suberin, keratin, horny carbon and mixtures thereof.

35. A skin care composition according to any one of claims 28 to 34, wherein The moisturizer is selected from glycerin, urea, hyaluronic acid, salicylic acid, alpha hydroxy acid, propylene glycol, honey, sorbitol, butylene glycol, sodium PCA, allantoin, sodium lactate, dicyandiamide, tremella extract, seaweed, aloe vera and a mixture thereof.

36. A skin care composition according to any one of claims 28 to 35, wherein The emollient is selected from the group consisting of petrolatum, lanolin, mineral oil, dimethicone, and mixtures thereof.

37. A skin care composition according to any one of claims 28 to 36, wherein The occlusive agent is selected from the group consisting of mineral oil, petrolatum, lanolin, polydimethylsiloxane, shea butter, and mixtures thereof.

38. A skin care composition according to any one of claims 28 to 37, wherein The ceramide is selected from the group consisting of ceramide EOS, ceramide NS, ceramide NG, ceramide NP, ceramide AP, ceramide EOP, phytosphingosine, sphingosine and mixtures thereof.

39. A skin care composition according to any one of claims 28 to 38, wherein The emulsifier is selected from the group consisting of sodium lauryl sulfate, dioctyl sodium sulfosuccinate, tragacanth gum, lecithin, mustard, soy lecithin, sodium phosphate, diacetyl tartaric acid ester of monoglyceride, sodium stearoyl lactylate and mixtures thereof.

40. A skin care composition according to any one of claims 28 to 39, wherein The thickener is selected from magnesium aluminum silicate, silicon dioxide, bentonite, carbomer, cetyl palmitate, ammonium acryloyldimethyltaurate and mixtures thereof.

41. A skin care composition according to any one of claims 28 to 40, wherein The preservative is selected from phenoxyethanol, benzyl alcohol, sodium benzoate, potassium sorbate, ethylhexylglycerin, DMDM ​​hydantoin, quaternium-15, imidazolidinyl urea, diazolidinyl urea, polyoxymethylene urea, sodium hydroxymethylglycinate, 2-bromo-2-nitropropane-1,3-diol (bronopol), glyoxal, ethyl parahydroxybenzoate, butyl parahydroxybenzoate, methyl parahydroxybenzoate, propyl parahydroxybenzoate, isobutyl parahydroxybenzoate, isopropyl parahydroxybenzoate, benzyl compounds, benzoic acid, benzyl esters, methylisothiazolinone (MIT; 2-methyl-4-isothiazolin-3-one), methylchloroisothiazolinone (CMIT; 5-chloro-2-methyl-4-isothiazolin-3-one), phenoxyethanol, 2-phenoxyethanol, EuxylK TM 400 (a mixture of phenoxyethanol and 1,2-dibromo-2,4-dicyanobutane), triclosan (TSC), triclocarban (TCC), benzyl alcohol, benzalkonium chloride, citric acid, dehydroacetic acid, essential oils, grapefruit seed extract, lactic acid, levulinic acid, potassium sorbate, sodium dehydroacetate, sodium metabisulfite, sodium salicylate, sorbic acid, vitamin E, zinc pyrithione and mixtures thereof.

42. A skin care composition according to any one of claims 28 to 41, wherein The antioxidant is selected from vitamin A, vitamin C, vitamin E, vitamin B3, green tea extract, resveratrol, curcumin, lycopene, coenzyme Q10, polyphenols, Centella asiatica, Tamanu and mixtures thereof.

43. A skin care composition according to any one of claims 28 to 42, wherein The fragrance is selected from linalool, citronellol, limonene, geraniol, eugenol, lavender oil (Lavandula angustifolia), rose extract (Rosa damascene), bergamot oil (Citrus bergamia), ylang-ylang oil (Canaga odorata), lemon (Citrus limon), lime (Citrus aurantifolia or Citron (Citrus medica)), orange (Citrus sinensis), citrus (Citrus tangerine), peppermint (Mentha piperita), spearmint (Menthaspicata), eucalyptus, cinnamon bark (cinnamomum), cinnamaldehyde and mixtures thereof.

44. A skin care composition according to any one of claims 28 to 43, wherein The functional ingredients are selected from biological extracts, vitamins, amino acids, peptides, proteins, essential fatty acids, hydroxy acids, β-glucan and mixtures thereof.

45. A skin care composition according to any one of claims 28 to 44, wherein The skin care composition is formulated as a paste, ointment, cream, lotion, gel and / or emulsion.

46. ​​Use of a skin care composition as defined in any one of claims 28 to 45 for benefiting the skin.

47. The use according to claim 46, which provides at least one of the following benefits to the skin: moisturizing the skin, improving the appearance of the skin, protecting from environmental damage, combating the effects of aging, removing wrinkles and / or sun spots.

48. A method of protecting the skin and / or improving the appearance of the skin comprising applying to the skin a skin care composition as defined in any one of claims 28 to 45.

49. A hair care composition comprising: a biopolymer and at least one of a surfactant, a conditioning surfactant, a co-surfactant, a conditioning softener, a thickener, an opacifier, a ceramide, a conditioning agent, a pH buffer, a chelating agent, a preservative, an emulsifier, a stabilizer, an antioxidant, a fragrance, and a functional ingredient.

50. A hair care composition according to claim 49 comprising a biopolymer, an emulsifier, a stabilizer and a preservative.

51. A hair care composition according to claim 49 or 50, wherein The composition comprises biopolymer molecules that have been mechanically processed into a stable homogeneous aqueous suspension.

52. A hair care composition according to any one of claims 49 to 51, wherein The composition comprises a homogenous aqueous suspension of insoluble and / or semi-soluble biopolymer particles.

53. The hair care composition according to claim 52, wherein The stable homogeneous aqueous suspension comprises insoluble and / or semi-soluble biopolymer particles stably dispersed in a polar solvent.

54. A hair care composition according to any one of claims 49 to 53, wherein The biopolymer consists of a stable homogeneous aqueous suspension comprising insoluble and / or semi-soluble biopolymer particles.

55. A hair care composition according to any one of claims 49 to 54, wherein The biopolymer is selected from the group consisting of chitin, chitosan, microcrystalline cellulose and microfibrillated cellulose.

56. A hair care composition according to any one of claims 49 to 54, wherein The surfactant is selected from the group consisting of lauryl sulfate, laureth sulfate, sarcosine, sulfosuccinate, betaine, alkyl amino acid, poloxamer, amine oxide, polyglucoside and mixtures thereof.

57. A hair care composition according to any one of claims 49 to 56, wherein The conditioning surfactant is selected from the group consisting of behenyltrimethylammonium chloride, stearyldimethylaminopropylamine, cetyltrimethylammonium chloride, stearalkonium chloride, and mixtures thereof.

58. A hair care composition according to any one of claims 49 to 57, wherein Cationic conditioning polymers selected from polyquaternium 7, 10, 53, 87, guar hydroxypropyltrimonium chloride, starch hydroxypropyltrimonium chloride, Sensomer TM CI-50 (33% active, Lubrizol), Cassia gelatin hydroxypropyltrimonium chloride, and mixtures thereof.

59. A hair care composition according to any one of claims 49 to 58, wherein The co-surfactant is selected from cetyl alcohol, stearyl alcohol, glyceryl stearate and a mixture thereof.

60. A hair care composition according to any one of claims 49 to 59, wherein The conditioning softener is selected from the group consisting of dimethicone, dimethiconol, trisiloxane, amodimethicone, polyricinoleic acid, polyester 37, trimethylolpropane tricaprylate / tricaprate, polyester-11, PPG-3 benzyl ether ethylhexanoate, and mixtures thereof.

61. A hair care composition according to any one of claims 49 to 60, wherein The hair care compositions include shampoos, rinse-off cream conditioners and / or spray conditioners.

62. Use of a hair care composition as defined in any one of claims 49 to 61 for washing hair, preventing hair damage and / or treating hair.

63. Use of a hair care composition as defined in any one of claims 49 to 61 in the manufacture of a shampoo, a rinse-off cream conditioner and / or a spray conditioner.

64. Use of a biopolymer for replacing microplastics in a cosmetic composition, the use comprising replacing at least a portion of the microplastics with a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent.

65. A method for replacing microplastics in a sunscreen composition, comprising: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; and (2) mixing the biopolymer suspension with at least one of a sensory enhancer, a film former, an emulsifier, an emollient, a UVB filter, a UVA filter, and a broad spectrum filter.

66. A method for replacing microplastics in a skin care composition, comprising: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; and (2) mixing the biopolymer suspension with at least one of a moisturizer, an emollient, an occlusive agent, a ceramide, an emulsifier, a thickener, a preservative, an antioxidant, a fragrance, and a functional ingredient.

67. A method for replacing microplastics in a hair care composition, comprising: (1) providing a biopolymer suspension comprising a suspension of insoluble and / or semi-soluble particles stably dispersed in a polar solvent; and (2) mixing the biopolymer suspension with at least one of a surfactant, a conditioning surfactant, a co-surfactant, a conditioning softener, a thickener, an opacifying agent, a ceramide, a conditioning agent, a pH buffer, a chelating agent, a preservative, an emulsifier, a stabilizer, an antioxidant, a fragrance, and a functional ingredient.

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