Liquid feeding and discharging device and method, immersion objective lens system, gene sequencer and biochemical detection method

Abstract

A liquid feeding and discharging device is used for applying working liquid from a liquid feeding station to a working surface of a platform assembly, the liquid feeding and discharging device can move relative to the working surface, and the liquid feeding and discharging device comprises a liquid feeding guide pipe assembly for applying the working liquid to the working surface; the liquid discharging guide pipe assembly is used for extracting the working liquid from the working surface; the catheter structure assembly is connected between the liquid feeding catheter assembly and the liquid discharging catheter assembly; a fluid interface assembly connected between the liquid supply station and the conduit structure assembly; the power assembly is connected with the fluid interface assembly and is configured to apply forward driving force to the liquid feeding guide pipe assembly through the fluid interface assembly so as to apply the working liquid to a working surface; or the reverse driving force is applied to the liquid discharging guide pipe assembly to pull the working liquid away from the working surface.

Description

Liquid supply and discharge device and method, immersion objective lens system, gene sequencer and biochemical detection method Technical Field

[0001] The present application relates to a liquid supply and discharge device and method, an immersion objective lens system, a gene sequencer and a biochemical detection method. Background Art

[0002] Immersion objectives are a common technique in photolithography, where the air between the optical lens and the target plane is replaced with an immersion fluid. The addition of an immersion fluid (such as water or oil) increases the refractive index (compared to air) and can improve the resolution of the objective.

[0003] The resolution (R) formula is as follows:

[0004]

[0005] Where λ is the wavelength of light used to image the specimen, and NA is the numerical aperture (NA) of the objective lens.

[0006] The numerical aperture formula is as follows:

[0007] NA=n×sinα

[0008] Where n is the refractive index of the medium through which the light passes, and α is the aperture angle of the given objective lens.

[0009] The resolution and numerical aperture formulas show that the objective's resolution is inversely proportional to the numerical aperture (NA) and the refractive index n of the medium. The refractive index of air is 1.0, the refractive index of water is 1.33, and the refractive index of glycerin is 1.47. Therefore, adding an immersion fluid between the optical lens and the target plane can increase the objective's numerical aperture (NA) and improve its resolution (R).

[0010] During extended use, immersion objectives require frequent replacement of the immersion fluid to ensure cleanliness and proper operation. In some applications, immersion objectives may need to be switched between two or more working surfaces, which in turn involves disengagement and contact between the objective and the immersion fluid. Therefore, automated liquid supply and drainage systems are crucial, ensuring that immersion fluid replacement is time-consuming. Furthermore, automated liquid supply and drainage methods adapted to immersion objectives of varying sizes can further enhance device compatibility and flexibility.

[0011] In existing structurally conductive immersion liquid feeding and removal methods, the immersion liquid addition structure is fixed to the objective lens and moves with it, becoming highly dependent on the objective lens structure and unable to adapt to different objective lenses. Furthermore, an existing pipeline-conducted, non-interfering transmission system for inverted microscopes can accurately feed and remove immersion liquid, but this system takes a considerable amount of time to perform the feeding and aspiration operations, during which the objective lens cannot be used. Another pipeline-conducted system has an immersion liquid feeding device fixed to the objective lens body, which is highly dependent on the objective lens structure and unable to adapt to different objective lenses. Furthermore, the objective lens barrel must be redesigned to facilitate feeding.

[0012] In summary, the existing liquid supply and drainage devices mainly have the following disadvantages:

[0013] (1) The size of the liquid supply and discharge module, which supports liquid supply and discharge functions, is strongly related to the structure of the objective lens and cannot match different objective lenses, lacking compatibility and flexibility;

[0014] (2) The objective lens needs to wait for a long time during liquid feeding and drainage, and cannot perform tasks such as taking pictures. Especially when the liquid feeding and drainage module is bound to the objective lens structure and the objective lens needs to be switched between two or more working surfaces or positions, there is a serial logical sequence of "liquid drainage---objective lens leaves the first working position---objective lens moves to the second working position---liquid feeding", which consumes a lot of time.

[0015] Summary of the Invention

[0016] The embodiments of the present application aim to solve at least one aspect of the above problems.

[0017] According to a first aspect of the present application, a liquid supply and drainage device is provided for applying working liquid from a liquid supply station to a working surface of a platform assembly, wherein the liquid supply and drainage device is movable relative to the working surface and comprises: a liquid supply conduit assembly for applying working liquid to the working surface; a liquid drainage conduit assembly for extracting the working liquid from the working surface; a conduit structure assembly connected between the liquid supply conduit assembly and the liquid drainage conduit assembly; a fluid interface assembly connected between the liquid supply station and the conduit structure assembly; and a power assembly connected to the fluid interface assembly, wherein the power assembly is configured to apply a positive driving force to the liquid supply conduit assembly via the fluid interface assembly to apply the working liquid to the working surface, or to apply a reverse driving force to the liquid drainage conduit assembly to extract the working liquid from the working surface.

[0018] According to the first aspect of the present application, the liquid supply conduit assembly is suspended on one side of the working surface and is opposite to and spaced apart from the working surface, and the liquid discharge conduit assembly is spaced apart from the liquid supply conduit assembly and suspended on the same side of the working surface.

[0019] According to the first aspect of the present application, the conduit structure assembly is integrally formed with the platform assembly, so that the openings of the liquid feeding conduit assembly and the liquid draining conduit assembly are located in the plane of the working surface.

[0020] According to the first aspect of the present application, the power assembly includes a driver connected between the liquid feeding station and the fluid interface assembly.

[0021] According to the first aspect of the present application, the fluid interface assembly includes a liquid supply pipeline that is fluidically connected to the liquid supply pipeline assembly via the catheter structure assembly, and a liquid discharge pipeline that is fluidically connected to the liquid discharge pipeline assembly via the catheter structure assembly, and the ends of the liquid supply pipeline and the liquid discharge pipeline that are away from the catheter structure assembly are respectively connected to the driver.

[0022] According to the first aspect of the present application, the catheter structure assembly is provided with a liquid supply interface connecting the liquid supply pipeline and the liquid supply catheter assembly, and a liquid discharge interface connecting the liquid discharge pipeline and the liquid discharge catheter assembly.

[0023] According to the first aspect of the present application, the power assembly further comprises a reversing assembly connected between the driver and one end of the liquid supply line away from the catheter structure assembly and one end of the liquid discharge line away from the catheter structure assembly, the reversing assembly having a first position for connecting the liquid supply line to the driver fluid and disconnecting the liquid discharge line from the driver fluid, and a second position for connecting the liquid discharge line to the driver fluid and disconnecting the liquid supply line from the driver fluid.

[0024] According to the first aspect of the present application, the fluid interface assembly further comprises a filter located in the drainage pipeline, and the fluid received by the drainage pipeline from the drainage conduit assembly is filtered by the filter and then discharged to the liquid feeding station through the reversing assembly.

[0025] According to the first aspect of the present application, the reversing assembly includes at least one of a solenoid valve, a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve, a rotary valve, and a rotary cutting valve.

[0026] According to the first aspect of the present application, the liquid supply and drainage device further includes a liquid drainage station, which is connected to the liquid drainage conduit assembly via the driver and the conduit structure assembly.

[0027] According to a first aspect of the present application, the driver includes a liquid feeding driver and a liquid discharging driver. The liquid feeding driver is connected between the liquid feeding pipeline and the liquid feeding station, and the liquid discharging driver is connected between the liquid discharging pipeline and the liquid discharging station.

[0028] According to the first aspect of the present application, the driver includes at least one of a peristaltic pump, a plunger pump, a syringe pump, a gear pump, a diaphragm pump, a vacuum pump, a diaphragm pump, and an air compressor.

[0029] According to the first aspect of the present application, the liquid supply drive is a first pump, and the liquid discharge drive is a second pump. The first pump and the second pump are connected to form a two-row pump. The two-row pump is configured so that the first pump drives the liquid supply conduit assembly to apply the working liquid to a first position on the working surface while the second pump drives the liquid discharge conduit assembly to extract the fluid located at a second position on the working surface; wherein the first position is opposite to the liquid supply conduit assembly, the second position is opposite to the liquid discharge conduit assembly, and the fluid includes gas located at the second position or waste liquid converted from the working liquid.

[0030] According to a first aspect of the present application, the liquid infusion driver is a first pump, the liquid expulsion driver is a second pump, and the first pump and the second pump operate independently of each other.

[0031] According to the first aspect of the present application, the first pump and the second pump are respectively located on different sides of the working surface.

[0032] According to the first aspect of the present application, the liquid feeding catheter assembly includes at least two liquid feeding catheters, the liquid feeding pipeline includes a liquid feeding sub-pipeline connected to the liquid feeding catheters in a one-to-one correspondence, and a common liquid feeding pipeline connected between the liquid feeding sub-pipeline and the first pump, and a first multi-way connector is connected between the common liquid feeding pipeline and the liquid feeding sub-pipeline.

[0033] According to the first aspect of the present application, the drainage conduit assembly includes at least two drainage conduits corresponding one-to-one to the liquid supply conduits, the drainage pipeline includes drainage sub-pipelines connected one-to-one to the drainage conduits, and a common drainage pipeline connected between the drainage sub-pipelines and the second pump, and a second multi-way connector is connected between the common drainage pipeline and the drainage sub-pipelines.

[0034] According to the first aspect of the present application, a first two-way valve is provided in at least one of the liquid feeding sub-pipelines.

[0035] According to the first aspect of the present application, a second two-way valve is provided in at least one of the liquid discharge sub-pipelines.

[0036] According to the first aspect of the present application, the liquid supply pipeline is provided with a sensor, which is configured to detect the quality of the working liquid flowing into the liquid supply pipeline through the driver. The fluid interface assembly also includes a bypass pipeline connected between the sensor and the liquid drainage station. The working liquid detected by the sensor as not meeting the quality requirements flows into the liquid drainage station through the bypass pipeline.

[0037] According to the first aspect of the present application, the fluid interface assembly further includes a third multi-way connector connecting the liquid discharge station, the liquid discharge driver, and the bypass line.

[0038] According to the first aspect of the present application, the liquid supply and drainage device further includes a cleaning station, which at least stores cleaning liquid for regularly cleaning the liquid supply pipeline.

[0039] According to a second aspect of the present application, an immersion objective lens system is disclosed, comprising a platform assembly having a working surface and an objective lens movable relative to the platform assembly, wherein the immersion objective lens system further comprises a liquid supply and drainage device as described in any one of claims 1 to 22, the liquid supply and drainage device providing working liquid as an immersion medium between the objective lens and the working surface.

[0040] According to a second aspect of the present application, the working surface includes a cleaning area, and the immersion objective lens system further includes a cleaning assembly connected to the cleaning area; the cleaning assembly includes a cleaning duct assembly extending to the cleaning area, and a cleaning power assembly connected to the cleaning duct assembly; the cleaning power assembly is configured to provide cleaning liquid to the cleaning area through the cleaning duct assembly to clean the objective lens when the objective lens moves above the cleaning area.

[0041] According to a second aspect of the present application, the cleaning conduit assembly includes a first cleaning conduit and a second cleaning conduit, the first cleaning conduit is configured to transport the cleaning liquid to the cleaning area, and the second cleaning conduit is configured to extract the cleaning liquid from the cleaning area.

[0042] According to a second aspect of the present application, the cleaning duct assembly further includes an air blowing duct, and the air blowing duct is used to dry the objective lens after being cleaned by the cleaning liquid.

[0043] According to a third aspect of the present application, a gene sequencer is disclosed, comprising a gene sequencing chip and an optical system for detecting the gene sequencing chip, wherein the optical system comprises an objective lens capable of moving relative to the gene sequencing chip, and a liquid supply and discharge device for providing an immersion medium between the objective lens and the gene sequencing chip, wherein the side of the gene sequencing chip close to the objective lens is a working surface; the liquid supply and discharge device is used to apply the immersion medium from a liquid supply station to the working surface and is capable of moving relative to the working surface, and the liquid supply and discharge device comprises: a liquid supply station suspended on one side of the working surface and in contact with the working surface; a liquid feeding conduit assembly facing each other and spaced apart; a liquid draining conduit assembly spaced apart from the liquid feeding conduit assembly and suspended on the same side of the working surface; a conduit structure assembly connected between the liquid feeding conduit assembly and the liquid draining conduit assembly; a fluid interface assembly connected between the liquid feeding station and the conduit structure assembly; and a power assembly connected to the fluid interface assembly, the power assembly being configured to apply a positive driving force to the liquid feeding conduit assembly via the fluid interface assembly to apply the immersion medium to the working surface, or to apply a reverse driving force to the liquid draining conduit assembly to extract the immersion medium from the working surface.

[0044] According to a fourth aspect of the present application, a gene sequencer for multiple chips is disclosed, the gene sequencer comprising at least two chip detection positions, the at least two chip detection positions being respectively used to install gene sequencing chips, the gene sequencer further comprising an optical system for detecting the gene sequencing chip, the optical system comprising an objective lens capable of moving relative to the gene sequencing chip, and a liquid supply and discharge device for providing an immersion medium between the objective lens and the gene sequencing chip, the side of each of the at least two gene sequencing chips close to the objective lens being a working surface; the liquid supply and discharge device being used to apply the immersion medium from a liquid supply station to the working surface and being capable of moving relative to the working surface, the liquid supply and discharge device comprising: a liquid supply station suspended on each of the working surfaces; and a liquid supply station for discharging liquid from the liquid supply station to the working surface. at least two liquid feeding conduits are arranged on one side of the surface and opposite to each of the working surfaces and spaced apart; at least two liquid drain conduits are spaced apart from and arranged in one-to-one correspondence with the at least two liquid feeding conduits and suspended on the same side of the working surface; a conduit structure assembly connected between the at least two liquid feeding conduits and the at least two liquid drain conduits; a fluid interface assembly connected between the liquid feeding station and the conduit structure assembly; and a power assembly connected to the fluid interface assembly, the power assembly being configured to apply a positive driving force to the at least two liquid feeding conduits via the fluid interface assembly so as to apply the immersion medium to each of the working surfaces via the at least two liquid feeding conduits, or to apply a reverse driving force to the at least two liquid drain conduits so as to extract the immersion medium from each of the working surfaces.

[0045] According to a fifth aspect of the present application, a liquid supply and discharge method for an objective lens is disclosed, comprising: a liquid supply step: suspending a liquid supply conduit assembly above an i-th liquid supply area of ​​a working surface having n liquid supply areas, i=1, 2, 3...n-1, n; a power assembly applying the working liquid from the liquid supply station to the i-th liquid supply area via a fluid interface assembly, a conduit structure assembly and a liquid supply conduit assembly in sequence; a detection step: driving the objective lens to move relative to the working surface so that the objective lens contacts the working liquid in the i-th liquid supply area and enters a working state and drives the objective lens to leave the i-th liquid supply area after the working state terminates; a liquid discharge step: suspending the liquid discharge conduit assembly above the i-th liquid supply area, and the power assembly extracting the waste liquid converted from the working liquid in the i-th liquid supply area from the i-th liquid supply area via the liquid discharge conduit assembly, the conduit structure assembly and the fluid interface assembly in sequence.

[0046] According to a fifth aspect of the present application, the liquid feeding step, the detection step and the liquid discharging step are performed in a cycle until the detection of the objective lens in the nth liquid feeding area is completed and the fluid located in the nth liquid feeding area is extracted.

[0047] According to the fifth aspect of the present application, the power component includes a liquid supply driver and a liquid discharge driver. When i≥2, the liquid discharge driver drives the liquid discharge catheter component to perform a liquid discharge step for the i-1 liquid supply area and the liquid supply driver drives the liquid supply catheter component to perform a liquid supply step for the i-th liquid supply area simultaneously.

[0048] According to the fifth aspect of the present application, the drainage step also includes: using the filter in the fluid interface assembly to filter the waste liquid drawn into the fluid interface assembly from the i-th liquid supply area and convert it into working liquid, and the power assembly returns the working liquid to the liquid supply station.

[0049] According to the fifth aspect of the present application, the power assembly includes a common driver, which first drives the drainage catheter assembly to perform the drainage step of the i-1th liquid supply area, and then drives the liquid supply catheter assembly to perform the liquid supply step of the i-th liquid supply area.

[0050] According to the fifth aspect of the present application, the liquid feeding drive is a first pump, the liquid discharge drive is a second pump, the first pump and the second pump are connected to each other to form a two-row pump, and the two-row pump is configured so that while the first pump drives the liquid feeding catheter assembly to perform the liquid feeding step in one of the liquid feeding areas, the second pump drives the liquid discharge catheter assembly to perform the liquid discharge step in another of the liquid feeding areas.

[0051] According to the fifth aspect of the present application, the second pump is connected between the drainage conduit assembly and the drainage station, and the drainage step also includes the second pump driving the waste liquid from the liquid feeding area into the fluid interface assembly to flow into the drainage station.

[0052] According to the fifth aspect of the present application, the liquid feeding step also includes a bubble detection step: using a bubble sensor provided in the fluid interface assembly to detect whether there are bubbles in the working liquid to be flowed into the liquid feeding conduit assembly via the bubble sensor, so that when bubbles are detected, the working liquid flows into the liquid discharge station via a bypass pipe connected between the bubble sensor and the liquid discharge station, and when no bubbles are detected, the working liquid flows into the liquid feeding conduit assembly via the bubble sensor.

[0053] According to a fifth aspect of the present application, the method further includes: an objective lens cleaning step: cleaning the objective lens using a cleaning assembly connected to the cleaning area of ​​the working surface, the cleaning assembly including a cleaning duct assembly extending to the cleaning area, and a cleaning power assembly connected to the cleaning duct assembly, the cleaning power assembly being configured to provide cleaning liquid to the cleaning area through the cleaning duct assembly to clean the objective lens when the objective lens moves above the cleaning area.

[0054] According to a sixth aspect of the present application, a liquid supply and discharge method for an objective lens is disclosed, wherein the objective lens is used for detecting a platform assembly including multiple platforms, and the multiple platforms respectively have working surfaces, wherein the method comprises: a liquid supply step: suspending at least two liquid supply conduit assemblies above the liquid supply areas of the at least two working surfaces, respectively, and a power assembly applying the working liquid from the liquid supply station to the liquid supply areas on the at least two working surfaces in sequence through the fluid interface assembly, the conduit structure assembly and the at least two liquid supply conduit assemblies; a detection step: driving the objective lens to move relative to the at least two working surfaces so that the objective lens successively contacts the working liquid in the liquid supply areas on the at least two working surfaces and enters a working state, and driving the objective lens to leave the liquid supply area after the working state terminates; and a liquid discharge step: after the objective lens leaves the liquid supply area, suspending the liquid discharge conduit assembly above the liquid supply area, and the power assembly extracting the fluid located in the liquid supply area from the liquid supply area in sequence through the liquid discharge conduit assembly, the conduit structure assembly and the fluid interface assembly.

[0055] According to the sixth aspect of the present application, the at least two liquid feeding conduit assemblies are supported by the same conduit structure assembly. During the liquid feeding step, the power assembly drives the working liquid to flow through the conduit structure assembly and simultaneously applies it to different working surfaces among the at least two working surfaces through the at least two liquid feeding conduit assemblies.

[0056] According to a sixth aspect of the present application, in the liquid feeding step, the power component sequentially drives the working liquid to be applied to the corresponding working surface of the at least two working surfaces through each of the at least two liquid feeding conduit components.

[0057] According to the sixth aspect of the present application, the drainage step also includes: using a filter to filter the waste liquid converted from the working liquid entering the fluid interface component from the liquid supply area into working liquid, and the power component returns the filtered working liquid to the liquid supply station.

[0058] According to a sixth aspect of the present application, the drainage step further includes: the power component drives the waste liquid converted from the working liquid from the liquid feeding area into the fluid interface component to flow into the drainage station.

[0059] According to the sixth aspect of the present application, the liquid feeding step also includes a bubble detection step: using a bubble sensor provided in the fluid interface assembly to detect whether there are bubbles in the working liquid to be flowed into the liquid feeding conduit assembly via the bubble sensor, so that when bubbles are detected, the working liquid flows into the liquid discharge station via a bypass line connected between the bubble sensor and the liquid discharge station, and when no bubbles are detected, the working liquid flows into the liquid feeding conduit assembly via the bubble sensor.

[0060] According to a sixth aspect of the present application, the method further includes an objective lens cleaning step: the objective lens is cleaned using a cleaning assembly connected to a cleaning area of ​​the working surface, the cleaning assembly including a cleaning duct assembly extending to the cleaning area, and a cleaning power assembly connected to the cleaning duct assembly, the cleaning power assembly being configured to provide cleaning liquid to the cleaning area through the cleaning duct assembly to clean the objective lens when the objective lens moves above the cleaning area.

[0061] According to the seventh aspect of the present application, a method for supplying and draining liquid for an objective lens is disclosed, comprising: a: placing a liquid supply conduit assembly in a conduit structure assembly formed integrally with a platform assembly, so that an opening of the liquid supply conduit assembly is located in an i-th liquid supply area of ​​the working surface having n liquid supply areas in the plane of the working surface of the platform assembly, i=1, 2, 3...n-1, n; a power assembly applies working liquid from a liquid supply station to the i-th liquid supply area via a fluid interface assembly, a liquid supply conduit assembly and a conduit structure assembly in sequence; b: placing a drainage conduit assembly in a conduit structure assembly formed integrally with the platform assembly, so that an opening of the drainage conduit assembly is located in an j-th drainage area of ​​the working surface having m drainage areas in the plane of the working surface of the platform assembly, j=1, 2, 3...m-1, m; c: driving the objective lens to move relative to the working surface so that the objective lens and the working liquid located in the i-th liquid supply area are in contact with each other. the working surface is in contact with the working liquid and enters a working state; d: after the working state is terminated, the objective lens and the waste liquid converted from the working liquid and attached to the objective lens are driven to leave the i-th liquid feeding area; the objective lens and the waste liquid are moved to just above the opening of the drainage duct assembly, and while the waste liquid is drawn away from the objective lens and the j-th drainage area by the drainage duct assembly, the liquid feeding duct assembly applies the working liquid to the i-th liquid feeding area of ​​the working surface; e: the objective lens is driven to move relative to the working surface so that the objective lens contacts the working liquid located in the i-th liquid feeding area and enters a working state; f: steps d to e are performed several times in a loop; g: after the working state is terminated, the objective lens and the waste liquid converted from the working liquid and attached to the objective lens are driven to leave the i-th liquid feeding area, the objective lens and the waste liquid are moved to just above the opening of the drainage duct assembly, and while the waste liquid is drawn away from the objective lens and the j-th drainage area by the drainage duct assembly.

[0062] According to an eighth aspect of the present application, a biochemical detection method is disclosed, comprising: loading a sample to be detected into a channel of a chip circulation pool; and loading a reagent having a plurality of different reaction components into the channel of the chip circulation pool to perform a biochemical reaction of the sample to be detected and the reagent, wherein the reaction components include at least one of a sample generating component and a sample analyzing component; optionally, the biochemical reaction comprises generating a sample in the channel of the chip circulation pool, generating the sample in the channel of the chip circulation pool comprises causing different sample generating components to flow into the channel and controlling the reaction conditions of the channel to generate the sample; and the biochemical reaction comprises analyzing the sample generated in the channel, analyzing the sample generated in the channel. Generating the sample includes allowing a sample analysis component to flow into the channel, the sample analysis component reacting with the generated sample to provide a relevant detectable signal; using a liquid supply conduit assembly to apply an immersion medium to a detection position corresponding to the relevant detectable signal located on the working surface; moving the optical system relative to the chip circulation pool to the detection position to contact the immersion medium and collect the relevant detectable signal; and separating the optical system from the immersion medium while using a liquid discharge conduit assembly to extract the immersion medium from the detection position; wherein the liquid supply conduit assembly and the liquid discharge conduit assembly are arranged at intervals and the liquid supply conduit assembly and the liquid discharge conduit assembly are interconnected by a conduit structure assembly.

[0063] According to the eighth aspect of the present application, the biochemical reaction is a nucleic acid sequencing reaction, and the sample to be detected is a nucleic acid sequencing library.

[0064] According to the eighth aspect of the present application, the detectable signal is an optical signal.

[0065] According to the eighth aspect of the present application, the sample to be detected is a tissue sample, and the biochemical reaction is a specific binding reaction. BRIEF DESCRIPTION OF THE DRAWINGS

[0066] The accompanying drawings, which are incorporated herein and form a part of the specification, illustrate the present disclosure and, together with the description, further serve to explain the principles of the present disclosure and enable one skilled in the relevant art to make and use the embodiments described herein.

[0067] FIG1 is a general block diagram showing a liquid supply and discharge device according to an embodiment of the present application;

[0068] FIG2 is a schematic diagram of a liquid supply and discharge device according to a first embodiment of the present application;

[0069] FIG3 is a schematic diagram of using the liquid supply and discharge device in FIG2 to discharge waste liquid in parallel;

[0070] FIG4 is a schematic diagram of using the liquid supply and discharge device in FIG2 to feed the working liquid in parallel;

[0071] FIG5 is a schematic diagram of a liquid supply and discharge device according to a second embodiment of the present application;

[0072] FIG6 is a schematic diagram of a liquid supply and discharge device according to a third embodiment of the present application;

[0073] FIG7 is a schematic diagram of a liquid supply and discharge device according to a fourth embodiment of the present application;

[0074] FIG8 is a schematic diagram of using the liquid supply and discharge device in FIG7 to discharge waste liquid in parallel;

[0075] FIG9 is a schematic diagram of a liquid supply and discharge device according to a fifth embodiment of the present application;

[0076] FIG10 is a schematic diagram of a liquid supply and discharge device according to a sixth embodiment of the present application;

[0077] FIG11 is a schematic diagram of a portion of a liquid supply and discharge device according to a seventh embodiment of the present application; and

[0078] FIG. 12 is a schematic diagram illustrating cleaning of the objective lens of the liquid supply and discharge device according to the first to seventh embodiments of the present application.

[0079] Features of the present disclosure will become more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which like reference numerals identify corresponding elements throughout. In the accompanying drawings, like reference numerals generally indicate identical, functionally similar, and / or structurally similar elements. Unless otherwise indicated, the drawings provided throughout this disclosure should not be construed as being drawn to scale. DETAILED DESCRIPTION

[0080] The technical solution of the present application is further described in detail below through examples and in conjunction with the accompanying drawings. In the specification, the same or similar reference numerals indicate the same or similar components. The following description of the embodiments of the present application with reference to the accompanying drawings is intended to explain the overall inventive concept of the present application and should not be construed as limiting the present application.

[0081] In addition, in the following detailed description, for ease of explanation, numerous specific details are set forth to provide a comprehensive understanding of the disclosed embodiments. However, it is apparent that one or more embodiments can be practiced without these specific details. In other cases, well-known structures and devices are shown in diagrammatic form to simplify the accompanying drawings.

[0082] Fig. 1 is a general block diagram of a liquid supply and discharge device according to an embodiment of the present application. Figs. 2 to 12 show liquid supply and discharge devices according to various specific embodiments of the present application.

[0083] 1 , a liquid supply and drainage device 1000 is provided for applying working liquid from a liquid supply station 112 to working surfaces 1051 and 1052 of a platform assembly 105. The liquid supply and drainage device 1000 is movable relative to the working surfaces 1051 and 1052. For clarity, FIG1 shows only two working surfaces 1051 and 1052. However, the present application is not limited thereto, and the liquid supply and drainage device 1000 may include more working surfaces.

[0084] The liquid supply and drainage device 1000 includes: a catheter structure component 101, a liquid supply catheter component 102, a liquid drainage catheter component 103, a fluid interface component 106, a power component 107, a liquid supply station 112, and a liquid drainage station 113.

[0085] The liquid supply conduit assembly 102 is suspended on one side of the working surfaces 1051, 1052 and is directly opposite to the working surfaces 1051, 1052 and is spaced apart. The liquid supply conduit assembly 102 includes n liquid supply conduits. The drainage conduit assembly 103 is spaced apart from the liquid supply conduit assembly 102 and suspended on the same side of the working surfaces 1051, 1052. The drainage conduit assembly 103 includes n drainage conduits. The liquid supply conduit or drainage conduit can be a metal tube, a plastic tube, etc. The conduit structure assembly 101 (which can be constructed as a plate or a block) is connected between the liquid supply conduit assembly 102 and the drainage conduit assembly 103 to define the relative positions of the liquid supply conduit assembly 102 and the drainage conduit assembly 103 and the platform assembly 105. The catheter structure assembly 101 is provided with a liquid supply interface 1011 connecting the liquid supply line 1061 with the liquid supply catheter assembly 102, and a liquid discharge interface 1012 connecting the liquid discharge line 1062 with the liquid discharge catheter assembly 103. A fluid interface assembly 106 is connected between the liquid supply station 112 and the catheter structure assembly 101. A power assembly 107 is connected to the fluid interface assembly 106 and is configured to apply a positive driving force to the liquid supply catheter assembly 102 via the fluid interface assembly 106 to apply the working liquid to the working surfaces 1051 and 1052, or to apply a negative driving force to the liquid discharge catheter assembly 103 to remove the working liquid from the working surfaces 1051 and 1052. Liquid supply includes continuous liquid addition and intermittent droplet addition. The liquid discharge station 113 is connected to the liquid discharge conduit assembly 103 via the power assembly 107, the fluid interface assembly 106, and the conduit structure assembly 101. The liquid feeding station 112 is connected to the liquid feeding conduit assembly 102 via the power assembly 107, the fluid interface assembly 106, and the conduit structure assembly 101.

[0086] As shown in FIG1 , the liquid supply and discharge device 1000 and the immersion objective lens 104 are relatively independent in structure. Therefore, the liquid supply and discharge device 1000 can be adapted to immersion objective lenses 104 of different sizes, eliminating the need to design different liquid supply and discharge devices for different sizes of immersion objective lenses 104. The liquid discharge conduit assembly 103 and the liquid supply conduit assembly 102 are spaced apart and suspended on the same side of the working surfaces 1051 and 1052, allowing the liquid discharge conduit assembly 103 and the liquid supply conduit assembly 102 to operate simultaneously, withdrawing working liquid from the working surfaces 1051 and 1052 while applying additional working liquid to the working surfaces 1051 and 1052. This allows the liquid supply and discharge operations to proceed in parallel, eliminating the need to wait for one operation to complete before proceeding to the other, saving time.

[0087] The steps for performing liquid feeding and draining operations using the liquid feeding and draining device 1000 are as follows:

[0088] a1: Use the liquid feeding conduit assembly 102 to add the working liquid in the liquid feeding station 112 to the platform assembly 105 .

[0089] a2: The platform assembly 105 and the immersion objective lens 104 move relative to each other, so that the working liquid is in full contact with the immersion objective lens 104, and the immersion objective lens 104 enters the working state.

[0090] a3: After the immersion objective lens 104 finishes working, the platform assembly 105 and the immersion objective lens 104 move relative to each other, so that the immersion objective lens 104 is separated from the waste liquid converted from the working liquid (that is, the objective lens 104 is immersed in the working liquid in the working state, causing the working liquid to be contaminated by impurities in the environment and become waste liquid). At the same time, the liquid supply conduit assembly 102 adds the working liquid in the liquid supply station 112 to the platform assembly 105.

[0091] a4: The platform assembly 105 and the immersion objective lens 104 move relative to each other, so that the drainage pipe assembly 103 contacts the working liquid on the platform assembly 105. While the drainage pipe assembly 103 removes the waste liquid, the working liquid fully contacts the immersion objective lens 104, and the immersion objective lens 104 enters the working state.

[0092] a5: Loop through steps a3 and a4 several times.

[0093] a6: After the immersion objective lens 104 is used, the platform assembly 105 and the immersion objective lens 104 move relative to each other, and the immersion objective lens 104 is separated from the waste liquid converted from the working liquid.

[0094] a7: The platform assembly 105 and the immersion objective lens 104 move relative to each other, and the drainage tube assembly 103 contacts the waste liquid on the platform assembly 105 to remove the waste liquid.

[0095] The liquid supply and drainage devices 1100 to 1700 are all different variations or specific implementations of the liquid supply and drainage device 1000 in FIG. 1 .

[0096] A liquid supply and discharge device 1100 according to a first embodiment of the present application will be described with reference to FIGS. 2 to 4 .

[0097] The liquid supply and drainage device 1100 includes a liquid supply station 112, a power assembly 107, a fluid interface assembly 106, a catheter structure assembly 101, a liquid supply catheter assembly 102 including a single liquid supply catheter 1021, and a liquid drainage catheter assembly 103 including a single liquid drainage catheter 1031. An immersion objective lens 104 is located between the liquid supply catheter 1021 and the liquid drainage catheter 1031. The liquid supply catheter 1021 and the liquid drainage catheter 1031 are located on the same side of a platform assembly 105 having a working surface 1051.

[0098] The power assembly 107 includes a driver 1071 connected between the liquid feeding station 112 and the fluid interface assembly 106. The driver 1071 is configured as a pump having two valve ports, one connected to the liquid feeding station 112 and the other connected to the fluid interface assembly 106. The pump includes, but is not limited to, at least one of a peristaltic pump, a plunger pump, a syringe pump, a gear pump, a diaphragm pump, a vacuum pump, a diaphragm pump, and an air compressor. The fluid interface assembly 106 includes a liquid feeding line 1061 in fluid communication with the liquid feeding line assembly 102 via the conduit structure assembly 101, and a liquid discharge line 1062 in fluid communication with the liquid discharge line assembly 103 via the conduit structure assembly 101. The ends of the liquid feeding line 1061 and the liquid discharge line 1062, which are distal to the conduit structure assembly 101, are respectively connected to the driver 1071. The power assembly 107 further includes a reversing assembly 1073 connected between the driver 1071 and an end of the liquid supply line 1061 remote from the catheter structure assembly 101 and an end of the liquid discharge line 1062 remote from the catheter structure assembly 101. The reversing assembly 1073 has a first position in which the liquid supply line 1061 is fluidically connected to the driver 1071 and the liquid discharge line 1062 is fluidically disconnected from the driver 1071, and a second position in which the liquid discharge line 1062 is fluidically connected to the driver 1071 and the liquid supply line 1061 is fluidically disconnected from the driver 1071. The reversing assembly 1073 includes, but is not limited to, at least one of a solenoid valve, a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve, a rotary valve, and a rotary cut valve.

[0099] The fluid interface assembly 106 also includes a filter 1063 located in the drainage pipe 1062. The fluid (i.e., the above-mentioned waste liquid) received by the drainage pipe 1062 from the drainage catheter assembly 103 is filtered out of impurities by the filter 1063 to become a working liquid. The filtered working liquid is discharged to the liquid supply station 112 through the reversing assembly 1073.

[0100] The steps for performing liquid feeding and draining operations using the liquid feeding and draining device 1100 are as follows:

[0101] b1: The driver 1071 extracts the working liquid 1120 from the liquid feeding station 112, and drips the working liquid 1120 into the platform assembly 105 through the liquid feeding pipeline 1061 (to prevent the liquid from hanging on the liquid feeding catheter 1021 and the catheter structure assembly 101, the driver 1071 can be operated to extract a certain volume of working liquid 1120 after the liquid feeding is completed).

[0102] b2: The platform assembly 105 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid 1120 and enters the working state.

[0103] b3: When the objective lens 104 finishes working, the platform assembly 105 and the objective lens 104 move relative to each other. At the same time, the objective lens 104 is separated from the waste liquid 1130 converted from the working liquid 1120, and the driver 1071 supplies liquid through the liquid supply pipeline 1061 and the liquid supply conduit 1021 (see Figure 4).

[0104] b4: The platform assembly 105 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid 1120 added in b3. At the same time, the drainage tube 1031 is in contact with the waste liquid 1130 (see Figure 3). The driver 1071 extracts the waste liquid 1130 on the platform assembly 105. The waste liquid 1130 is filtered through the filter 115 to become the working liquid 1120, and the working liquid 1120 formed by the filtration is discharged to the liquid supply station 112 through the driver 1071.

[0105] b5: The platform assembly 105 and the objective lens 104 move relative to each other, and the objective lens 104 enters the working state.

[0106] b6: Loop through steps b3 to b5 several times.

[0107] b7: After the objective lens 104 has been used, the platform assembly 105 and the objective lens 104 move relative to each other, and the objective lens 104 is separated from the waste liquid 1130.

[0108] b8: The platform assembly 105 and the objective lens 104 move relative to each other, the drainage tube 1031 contacts the waste liquid 1130, and the driver 1071 extracts the waste liquid 1130 on the platform assembly 105. The waste liquid 1130 is filtered through the filter 115, and the working liquid 1120 formed by the filtration is discharged to the liquid supply station 112 through the driver 1071.

[0109] As can be seen from the above, the liquid supply and discharge device 1100 can not only perform liquid supply and liquid discharge operations simultaneously, saving working time, but also recycle the working liquid, reducing material costs.

[0110] 5 , a liquid supply and discharge device 1200 according to a second embodiment of the present application will be described.

[0111] The driver 1071 in the liquid supply and discharge device 1100 is used for both liquid supply and liquid discharge. In contrast, the driver 1071 in the liquid supply and discharge device 1200 is only used for liquid supply. The liquid supply and discharge device 1200 includes not only a liquid supply driver 1071 but also a liquid discharge driver 1072. The liquid supply driver 1071 is connected between the liquid supply pipeline 1061 and the liquid supply station 112, and the liquid discharge driver 1072 is connected between the liquid discharge pipeline 1062 and the liquid discharge station 113. The liquid supply drive 1071 is configured as a first pump, and the liquid discharge drive 1072 is configured as a second pump. The first pump and the second pump are connected to form a two-row pump, so that the first pump and the second pump can operate synchronously. The two-row pump is configured so that the first pump drives the liquid supply conduit assembly 102 (i.e., the liquid supply conduit 1021) to apply working liquid to a first location on the working surface 1051, while the second pump drives the liquid discharge conduit assembly 103 (i.e., the liquid discharge conduit 1031) to extract fluid at a second location on the working surface. The first location is opposite to the liquid supply conduit assembly 102 (i.e., the liquid supply conduit 1021), and the second location is opposite to the liquid discharge conduit assembly 103 (i.e., the liquid discharge conduit 1031). The fluid includes gas at the second location or waste liquid converted from the working liquid. The relative position between the liquid supply conduit 1021 and the liquid discharge conduit 1031 is defined by the conduit structure assembly 101. In this embodiment, two coupled pumps are used to synchronize the extraction of waste liquid from the platform assembly 105 and the extraction of working liquid from the liquid supply station 112, and the liquid supply to the platform assembly 105 and the discharge of waste liquid to the liquid discharge station 113 are synchronized, further simplifying the operating steps of the liquid discharge device 1200.

[0112] The steps for performing liquid feeding and draining operations using the liquid feeding and draining device 1200 are as follows:

[0113] c1: While the first pump extracts the working liquid from the liquid supply station 112, the second pump extracts the air on the platform assembly 105. While the first pump drips the working liquid to the platform assembly 105 through the liquid supply conduit 1021, the second pump discharges the air to the liquid discharge station 113 through the liquid discharge conduit 1031.

[0114] c2: The platform assembly 105 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid and enters the working state.

[0115] c3: After the objective lens 104 finishes working, the platform assembly 105 and the objective lens 104 move relative to each other. While the objective lens 104 is separated from the waste liquid converted from the working liquid, the first pump drips the working liquid into the platform assembly 105 through the liquid supply conduit 1021 and the second pump discharges the air to the liquid discharge station 113.

[0116] c4: The platform assembly 105 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid and the drainage pipe 1031 is in contact with the waste liquid, so that the first pump extracts the working liquid from the liquid supply station 112 while the second pump extracts the waste liquid.

[0117] c5: The platform assembly 105 and the objective lens 104 move relative to each other, and the objective lens enters the working state.

[0118] c6: When the objective lens 104 finishes working, the platform assembly 105 and the objective lens 104 move relative to each other. At the same time, the objective lens 104 is separated from the waste liquid converted from the working liquid. The first pump supplies liquid through the liquid supply conduit 1021 and the second pump discharges the waste liquid extracted in step c4 to the liquid discharge station 113.

[0119] c7: Loop through steps c4 to c6 several times.

[0120] c8: The platform assembly 105 and the objective lens 104 move relative to each other, so that the objective lens 104 is fully in contact with the working liquid added in step c6 and enters a working state.

[0121] c9: After the objective lens 104 is used up, the platform assembly 105 and the objective lens 104 move relative to each other, and the objective lens 104 is separated from the waste liquid converted from the working liquid.

[0122] c10: The platform assembly 105 and the objective lens 104 move relative to each other, and the drainage tube 1031 contacts the waste liquid used in step c9, so that the second pump extracts the waste liquid.

[0123] Referring to FIG6 , a liquid supply and discharge device 1300 according to a third embodiment of the present application is described. For the sake of clarity, the following description focuses only on the differences between the liquid supply and discharge device 1300 and the liquid supply and discharge device 1200. The liquid supply drive 1071 of the liquid supply and discharge device 1300 is configured as a first pump, and the liquid discharge drive 1072 of the liquid supply and discharge device 1300 is configured as a second pump. The first pump and the second pump operate independently of each other, so that the liquid supply and discharge operations can be performed independently of each other. The first pump and the second pump are respectively located on the left and right sides of the working surface 1051.

[0124] The steps for performing liquid feeding and draining operations using the liquid feeding and draining device 1300 are as follows:

[0125] d1: The first pump extracts the working liquid from the liquid supply station 112 and drips the working liquid into the platform assembly 105 through the liquid supply conduit 1021 .

[0126] d2: The platform assembly 105 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid and enters the working state.

[0127] d3: The objective lens 104 finishes working, and the platform assembly 105 and the objective lens 104 move relative to each other. At the same time, the objective lens 104 is separated from the waste liquid converted from the working liquid. At the same time, the first pump drips the working liquid into the platform assembly 105 through the liquid supply conduit 1021.

[0128] d4: the platform assembly 105 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid on the platform assembly 105 . At the same time, the drainage pipe 1031 is in contact with the waste liquid, and the second pump discharges the waste liquid to the drainage station 113 .

[0129] d5: The platform assembly 105 and the objective lens 104 move relative to each other, and the objective lens 104 enters the working state.

[0130] d6: Loop through steps d3 to d5 several times.

[0131] d7: The objective lens 104 is finished being used, and the platform assembly 105 and the objective lens 104 move relative to each other, and the objective lens 104 is separated from the waste liquid.

[0132] d8: The platform assembly 105 and the objective lens 104 further move relative to each other, the drainage conduit 1031 comes into contact with the waste liquid, and the second pump discharges the waste liquid to the drainage station 113 .

[0133] A liquid supply and drainage device 1400 according to a fourth embodiment of the present application will be described with reference to Figures 7 and 8. For the sake of clarity, the following description will focus on only the differences between the liquid supply and drainage device 1400 and the liquid supply and drainage device 1300.

[0134] The liquid feeding conduit assembly 102 includes two liquid feeding conduits 1021 and 1022. The liquid feeding pipeline 1061 includes liquid feeding sub-pipelines 10611 and 10612 that are in one-to-one communication with the liquid feeding conduits 1021 and 1022, and a common liquid feeding pipeline 10613 connected between the liquid feeding sub-pipelines 10611 and 10612 and the first pump. A first multi-way connector 109 is connected between the common liquid feeding pipeline 10613 and the liquid feeding sub-pipelines 10611 and 10612. The first multi-way connector 109 has a first end 1091 connected to the liquid feeding sub-pipeline 10611, a second end 1092 connected to the liquid feeding sub-pipeline 10612, and a third end 1093 connected to the common liquid feeding pipeline 10613. The liquid supply sub-pipeline 10611 is connected between the first end 1091 and the liquid supply interface 1011 , and the liquid supply sub-pipeline 10612 is connected between the second end 1092 and the liquid supply interface 1013 .

[0135] The drainage conduit assembly 103 includes two drainage conduits 1031 and 1032 corresponding one-to-one with the liquid supply conduits 1021 and 1022. The drainage pipeline 1062 includes drainage sub-pipelines 10621 and 10622 corresponding one-to-one with the drainage conduits 1031 and 1032, and a common drainage pipeline 10623 connected between the drainage sub-pipelines 10621 and 10622 and the second pump. A second multi-way connector 110 is connected between the common drainage pipeline 10623 and the drainage sub-pipelines 10621 and 10622. The second multi-way connector 110 has a first end 1101 connected to the drainage sub-pipeline 10621, a second end 1102 connected to the drainage sub-pipeline 10622, and a third end 1103 connected to the common drainage pipeline 10623. The drainage sub-pipeline 10621 is connected between the first end 1101 and the drainage interface 1012, and the drainage sub-pipeline 10622 is connected between the second end 1102 and the drainage interface 1014. The objective lens 104 is located between the drainage conduit 1031 and the liquid supply conduit 1022.

[0136] Platform assembly 105 has two working surfaces 1051 and 1052. A liquid supply conduit 1021 and a liquid drainage conduit 1031 serve working surface 1051, while a liquid supply conduit 1022 and a liquid drainage conduit 1032 serve working surface 1052. Two-way valves 114 are respectively provided in drainage sub-pipelines 10621 and 10622. Liquid supply and drainage device 1400 enables operations on both working surfaces 1051 and 1052. The provision of two-way valves 114 enables drainage operations to be performed on each of working surfaces 1051 and 1052, improving the specificity and independence of drainage operations on working surfaces 1051 and 1052.

[0137] The steps for performing liquid feeding and draining operations using the liquid feeding and draining device 1400 are as follows:

[0138] e1: The first pump extracts the working liquid 1120 from the liquid supply station 112 , and the first pump drips the working liquid 1120 onto the working surfaces 1051 and 1052 through the liquid supply conduits 1021 and 1022 , respectively.

[0139] e2: The working surface 1051 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid 1120 on the working surface 1051 and enters a working state.

[0140] e3: The objective lens 104 finishes working, the working surface 1051 and the objective lens 104 move relative to each other, and the objective lens 104 and the waste liquid 1130 converted from the working liquid 1120 on the working surface 1051 are separated.

[0141] e4: The working surfaces 1051, 1052 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid 1120 on the working surface 1052. At the same time, the drainage conduit 1031 is in contact with the waste liquid 1130 on the working surface 1051, the two-way valve 114 on the drainage sub-pipeline 10621 is opened, and the waste liquid 1130 on the working surface 1051 is discharged to the drainage station 113 through the second pump.

[0142] e5: The working surface 1052 and the objective lens 104 move relative to each other, and the objective lens enters the working state.

[0143] e6: When the objective lens 104 finishes working, the working surface 1052 and the objective lens 104 move relative to each other. While the waste liquid 1130 converted from the working liquid 1120 on the objective lens 104 and the working surface 1052 are separated, the first pump drips the working liquid 1120 onto the working surfaces 1051 and 1052 through the liquid supply conduits 1021 and 1022 respectively.

[0144] e7: The working surfaces 1051, 1052 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid 1120 on the working surface 1051, and the drainage conduit 1032 is in contact with the waste liquid 1130 on the working surface 1052 (see Figure 8), the two-way valve 114 on the drainage sub-pipeline 10622 is opened, and the waste liquid 1130 on the working surface 1052 is discharged to the drainage station 113 through the second pump.

[0145] e8: Loop through steps e2 to e7 several times.

[0146] e9: When the objective lens 104 finishes working, the working surfaces 1051, 1052 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid 1120 on the working surface 1052. At the same time, the drainage conduit 1031 is in contact with the waste liquid 1130 converted from the working liquid 1120 on the working surface 1051. The two-way valve 114 on the drainage sub-pipeline 10621 is opened, and the waste liquid 1130 on the working surface 1051 is discharged to the drainage station 113 through the second pump.

[0147] e10: After the objective lens 104 is used, the working surface 1052 and the objective lens 104 move relative to each other. After the objective lens 104 and the waste liquid 1130 converted from the working liquid 1120 on the working surface 1052 are separated, the drainage conduit 1032 contacts the waste liquid 1130 on the working surface 1052, the two-way valve 114 on the drainage sub-pipeline 10622 is opened, and the waste liquid 1130 on the working surface 1052 is discharged to the drainage station 113 through the second pump.

[0148] 9 , a fifth embodiment of a liquid supply and drainage device 1500 is described. The liquid supply and drainage device 1500 is similar to the liquid supply and drainage device 1400 in many aspects. For clarity, the following description focuses on the differences between the liquid supply and drainage device 1500 and the liquid supply and drainage device 1400.

[0149] Two-way valves 111 are respectively provided in the liquid supply sub-pipelines 10611 and 10612. The liquid supply and discharge device 1500 realizes operation on the two working surfaces 1051 and 1052. The provision of the two-way valves 111 enables liquid supply operations to be performed on the working surfaces 1051 and 1052 respectively, improving the pertinence and independence of the liquid supply operations on the working surfaces 1051 and 1052.

[0150] The steps for performing liquid feeding and draining operations using the liquid feeding and draining device 1500 are as follows:

[0151] f1: The first pump draws working liquid from the liquid supply station 112 , opens the two-way valve 111 on the liquid supply sub-pipeline 10611 , and drips the working liquid onto the working surface 1051 through the liquid supply conduit 1021 .

[0152] f2: the working surface 1051 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid on the working surface 1051 .

[0153] f3: the working surface 1051 and the objective lens 104 move relative to each other, and the objective lens 104 enters the working state.

[0154] f4: The objective lens 104 finishes working, and the working surface 1051 and the objective lens 104 move relative to each other. While the objective lens 104 is separated from the waste liquid converted from the working liquid, the two-way valve 111 on the liquid supply sub-pipeline 10612 is opened, and the first pump drips the working liquid onto the working surface 1052 through the liquid supply conduit 1022.

[0155] f5: The working surfaces 1051, 1052 and the objective lens 104 move relative to each other, so that the objective lens 104 is in full contact with the working liquid on the working surface 1052. At the same time, the drainage conduit 1031 is in contact with the waste liquid on the working surface 1051, the two-way valve 114 on the drainage sub-pipeline 10621 is opened, and the waste liquid on the working surface 1051 is discharged to the drainage station 113 through the second pump.

[0156] f6: The working surface 1052 and the objective lens 104 move relative to each other, and the objective lens 104 enters the working state.

[0157] f7: When the objective lens 104 finishes working, the working surface 1052 and the objective lens 104 move relative to each other. While the waste liquid converted from the working liquid on the objective lens 104 and the working surface 1052 are separated, the two-way valve 111 on the liquid supply sub-pipeline 10611 is opened, allowing the first pump to drip the working liquid onto the working surface 1051 through the liquid supply conduit 1021.

[0158] f8: The working surfaces 1051 and 1052 move relative to the objective lens 104, so that the objective lens 104 is in full contact with the working liquid on the working surface 1051. At the same time, the drainage conduit 1032 is in contact with the waste liquid on the working surface 1052. The two-way valve 114 on the drainage sub-pipeline 10622 is opened, so that the second pump discharges the waste liquid on the working surface 1052 to the drainage station 113.

[0159] f9: Loop through steps f3 to f8 for several times.

[0160] f10: the working surface 1051 and the objective lens 104 move relative to each other, and the objective lens 104 enters the working state.

[0161] f11: The objective lens 104 is finished being used, and the working surface 1051 and the objective lens 104 move relative to each other, and the waste liquid converted from the working liquid on the objective lens 104 and the working surface 1051 are separated.

[0162] f12: The working surfaces 1051 and 1052 move relative to the objective lens 104, the drainage conduit 1031 contacts the waste liquid on the working surface 1051, the two-way valve 114 on the drainage sub-pipeline 10621 is opened, and the waste liquid on the working surface 1051 is discharged to the drainage station 113 through the second pump.

[0163] 10 , a sixth embodiment of a liquid supply and drainage device 1600 is described. The liquid supply and drainage device 1600 is similar to the liquid supply and drainage device 1300 in many aspects. For clarity, the following description focuses on the differences between the liquid supply and drainage device 1600 and the liquid supply and drainage device 1300.

[0164] The liquid supply line 1061 is provided with a sensor 115, configured to detect the quality of the working liquid flowing into the liquid supply line 1061 via the actuator 1071 (e.g., whether there are bubbles). The fluid interface assembly 106 also includes a bypass line 1064 connected between the sensor 115 and the liquid discharge station 113, and a third multi-way connector 116 connected between the bypass line 1064, the liquid discharge station 113, and the liquid discharge actuator 1072. The third multi-way connector 116 includes a first end 1161 connected to the bypass line 1064, a second end 1162 connected to the liquid discharge actuator 1072, and a third end 1163 connected to the liquid discharge station 113. Working liquid detected by the sensor 115 as substandard quality is allowed to flow into the liquid discharge station 113 via the bypass line 1064, which is essential for applications requiring high working liquid quality.

[0165] Except for using the sensor 115 to detect the quality of the working fluid to be fed onto the working surface 1051 , other steps of performing the fluid supply and drainage operation using the fluid supply and drainage device 1600 are similar to those of the fluid supply and drainage device 1300 and are not repeated here.

[0166] A seventh embodiment of the present application will be described with reference to Figure 11. The liquid supply and drainage device 1700 is identical to the liquid supply and drainage devices 1100 to 1600 in many respects. For clarity, the following description will focus on the differences between the liquid supply and drainage device 1700 and the liquid supply and drainage devices 1100 to 1600.

[0167] The conduit structure assembly 101 is integrally formed with the platform assembly 105, which simplifies the construction of the liquid supply and drainage device and allows the openings of the liquid supply conduit assembly 102 and the liquid drainage conduit assembly 103 to be located in the plane of the working surface 1051. Because the conduit structure assembly 101 is integrally formed with the platform assembly 105, only the platform assembly 105 is shown in FIG11. For ease of description, FIG11 only shows the case where the liquid supply conduit assembly 102 includes a single liquid supply conduit 1021 and the liquid drainage conduit assembly 103 includes a single liquid drainage conduit 1031. However, the present application is not limited to this, and it is also possible that the platform assembly 105 includes multiple working surfaces, the liquid supply conduit assembly 102 includes multiple liquid supply conduits, and the liquid drainage conduit assembly 103 includes multiple liquid drainage conduits.

[0168] The steps for performing liquid feeding and draining operations using the liquid feeding and draining device 1700 are as follows:

[0169] g1: Working liquid 1120 is added to the working surface 1051 through the liquid supply conduit 1021 .

[0170] g2: There is relative movement between the working surface 1051 and the objective lens 104, the working liquid 1120 is in full contact with the objective lens 104, and the objective lens 104 enters the working state.

[0171] g3: After the objective lens 104 finishes working, the working surface 1051 and the objective lens 104 move relative to each other, and the waste liquid 1130 converted from the working liquid 1120 is driven by the objective lens 104 to the top of the opening of the drainage duct 1031. At the same time, the waste liquid 1130 is drawn away from the objective lens 104 and discharged by the drainage duct 1031, and the liquid supply duct 1021 adds the working liquid 1120 to the working surface 1051.

[0172] g4: There is relative movement between the working surface 1051 and the objective lens 104, the working liquid 1120 is in full contact with the objective lens 104, and the objective lens 104 enters the working state.

[0173] g5: Loop through steps g3 to g4 several times.

[0174] g6: After the objective lens 104 is used up, the working surface 1051 and the objective lens 104 move relative to each other, and the waste liquid 1130 converted from the working liquid 1120 is driven by the objective lens 104 to the top of the opening of the drainage duct 1031. The waste liquid 1130 is drawn away from the objective lens 104 by the drainage duct 1031 and discharged.

[0175] The liquid supply and drainage devices 1100 to 1700 further include a cleaning station (see FIG. 12 ), which stores at least a cleaning liquid for regularly cleaning the liquid supply pipeline 1061 .

[0176] For the sake of clarity, the following description will use the working surface 1051 of the liquid supply and discharge device 1100 as an example to describe the cleaning area 10511 of the cleaning station (the area enclosed by the dotted box in the figure). However, the present application is not limited thereto, and the working surface 1052 may also have a cleaning area. The immersion objective lens system further includes a cleaning assembly 108 connected to the cleaning area 10511; the cleaning assembly 108 includes a cleaning conduit assembly 1081 extending to the cleaning area 10511, and a cleaning power assembly (not shown in the figure) connected to the cleaning conduit assembly 1081; the cleaning power assembly is configured to supply cleaning liquid to the cleaning area 10511 via the cleaning conduit assembly 1081 when the objective lens 104 moves above the cleaning area 10511 to clean the objective lens 104.

[0177] The cleaning conduit assembly 1081 includes a first cleaning conduit 10811 and a second cleaning conduit 10812. The first cleaning conduit 10811 is configured to transport the cleaning liquid to the cleaning area 10511, and the second cleaning conduit 10812 is configured to extract the cleaning liquid from the cleaning area 10511. The cleaning conduit assembly 108 also includes an air blowing conduit 10813 located between the first cleaning conduit 10811 and the second cleaning conduit 10812. The air blowing conduit 10813 is used to dry the objective lens 104 after being cleaned with the cleaning liquid.

[0178] The present application also discloses an immersion objective lens system, comprising a platform assembly 105 having working surfaces 1051 and 1052, and an objective lens 104 movable relative to the platform assembly 105, wherein the immersion objective lens system further comprises the liquid supply and drainage devices 1100 to 1700 as described above, and the liquid supply and drainage devices 1100 to 1700 provide working liquid 1120 as an immersion medium between the objective lens 104 and the working surfaces 1051 and 1052.

[0179] The present application also discloses a gene sequencer (not shown in the figure), including a gene sequencing chip and an optical system for detecting the gene sequencing chip, wherein the optical system includes an objective lens 104 capable of moving relative to the gene sequencing chip, and the above-mentioned liquid supply and discharge devices 1100 to 1300 and 1600 for providing immersion medium between the objective lens 104 and the gene sequencing chip, and the side of the gene sequencing chip close to the objective lens 104 is a working surface 1051.

[0180] The liquid supply and discharge devices 1100 to 1300 and 1600 are used to apply the immersion medium from the liquid supply station 112 to the working surface 1051 and are movable relative to the working surface 1051. The liquid supply and discharge devices 1100 to 1300 and 1600 include: a liquid supply conduit assembly 102 suspended on one side of the working surface 1051 and facing the working surface 1051 and spaced apart; a liquid discharge conduit assembly 103 spaced apart from the liquid supply conduit assembly 102 and suspended on the same side of the working surface 1051; and a liquid discharge conduit assembly 103 connected to the liquid supply conduit assembly 102. 02 and the drainage conduit assembly 103; a fluid interface assembly 106 connected between the liquid feeding station 112 and the conduit structure assembly 101; and a power assembly 107 connected to the fluid interface assembly 106, the power assembly 107 being configured to apply a positive driving force to the liquid feeding conduit assembly 102 via the fluid interface assembly 106 to apply the immersion medium to the working surface 1051, or to apply a reverse driving force to the drainage conduit assembly 103 to remove the immersion medium from the working surface 1051.

[0181] The present application also discloses a gene sequencer for multiple chips (not shown in the figure), the gene sequencer includes at least two chip detection positions, the at least two chip detection positions are respectively used to install gene sequencing chips, the gene sequencer also includes an optical system for detecting the gene sequencing chip, the optical system includes an objective lens 104 that can move relative to the gene sequencing chip, and a liquid supply and discharge device 1400 to 1500 for providing an immersion medium between the objective lens 104 and the gene sequencing chip, and each of the at least two gene sequencing chips has a side close to the objective lens 104 as a working surface 1051, 1052; the liquid supply and discharge device 1400 to 1500 is used to apply the immersion medium from the liquid supply station 112 to the working surfaces 1051, 1052 and can move relative to the working surfaces 1051, 1052, and the liquid supply and discharge device 1400 to 1500 includes: a liquid supply station 112 suspended on one side of each of the working surfaces 1051, 1052 and connected to each of the working surfaces 1051, 1052 At least two liquid supply conduits 1021, 1022 are arranged opposite and spaced apart; at least two liquid discharge conduits 1031, 1032 are spaced apart and arranged one-to-one with the at least two liquid supply conduits 1021, 1022 and suspended on the same side of the working surfaces 1051, 1052; a conduit structure assembly 101 connected between the at least two liquid supply conduits 1021, 1022 and the at least two liquid discharge conduits 1031, 1032; a fluid interface assembly 101 connected between the liquid supply station 112 and the conduit structure assembly 101 6; and a power assembly 107 connected to the fluid interface assembly 106, the power assembly 107 being configured to apply a positive driving force to the at least two liquid supply conduits 1021, 1022 via the fluid interface assembly 106 to apply the immersion medium to each of the working surfaces 1051, 1052 via the at least two liquid supply conduits 1021, 1022, respectively, or to apply a reverse driving force to the at least two liquid discharge conduits 1031, 1032 to extract the immersion medium from each of the working surfaces 1051, 1052.

[0182] According to the steps of performing liquid supply and discharge operations using the liquid supply and discharge device 1000 to 1600, the present application further discloses a liquid supply and discharge method for an objective lens 104, comprising: a liquid supply step: suspending the liquid supply conduit assembly 102 above the i-th liquid supply area of ​​a working surface 1051 having n liquid supply areas, i=1, 2, 3...n-1, n; the power assembly 107 applies the working liquid 1120 from the liquid supply station 112 to the i-th liquid supply area in sequence through the fluid interface assembly 106, the conduit structure assembly 101 and the liquid supply conduit assembly 102; a detection step: driving the objective lens 1104 to move the working liquid 1120 to the i-th liquid supply area; 04 moves relative to the working surface 1051, so that the objective lens 104 contacts the working liquid 1120 located in the i-th liquid feeding area and enters a working state and drives the objective lens 104 to leave the i-th liquid feeding area after the working state is terminated; drainage step: suspending the drainage catheter assembly 103 above the i-th liquid feeding area, and the power assembly 107 extracts the waste liquid 1130 converted from the working liquid 1120 located in the i-th liquid feeding area from the i-th liquid feeding area through the drainage catheter assembly 103, the catheter structure assembly 101 and the fluid interface assembly 106 in sequence.

[0183] The liquid feeding step, the detection step, and the liquid draining step are executed in a cycle until the detection of the objective lens 104 in the nth liquid feeding area is completed and the waste liquid 1130 in the nth liquid feeding area is extracted.

[0184] The power component 107 includes a liquid supply driver 1071 and a liquid discharge driver 1072. When i≥2, the liquid discharge driver 1072 drives the liquid discharge catheter component 103 to perform a liquid discharge step for the i-1 liquid supply area, and the liquid supply driver 1071 drives the liquid supply catheter component 102 to perform a liquid supply step for the i-th liquid supply area at the same time.

[0185] The drainage step also includes: using the filter 1063 in the fluid interface component 106 to filter the waste liquid 1130 drawn into the fluid interface component 106 from the i-th liquid supply area and convert it into a working liquid 1120, and the power component 107 returns the working liquid 1120 to the liquid supply station 112.

[0186] The power assembly 107 includes a common driver 1071. The common driver 1071 first drives the drainage conduit assembly 103 to perform the drainage step of the i-1th liquid feeding area, and then drives the liquid feeding conduit assembly 102 to perform the liquid feeding step of the i-th liquid feeding area.

[0187] The liquid feeding drive 1071 is configured as a first pump, and the liquid discharge drive 1072 is configured as a second pump. The first pump and the second pump are connected to each other to form a two-row pump. The two-row pump is configured so that while the first pump drives the liquid feeding catheter assembly 102 to perform the liquid feeding step in one liquid feeding area, the second pump drives the liquid discharge catheter assembly 103 to perform the liquid discharge step in another liquid feeding area.

[0188] The second pump is connected between the drainage conduit assembly 103 and the drainage station 113 . The drainage step further includes the second pump driving the waste liquid 1130 from the liquid feeding area into the fluid interface assembly 106 to flow into the drainage station 113 .

[0189] The liquid feeding step also includes a bubble detection step: using the bubble sensor 115 set in the fluid interface assembly 106 to detect whether there are bubbles in the working liquid 1120 to be flowed into the liquid feeding catheter assembly 102 through the bubble sensor 115, so that when bubbles are detected, the working liquid 1120 flows into the liquid discharge station 113 through the bypass line 1064 connected between the bubble sensor 115 and the liquid discharge station 113, and when no bubbles are detected, the working liquid 1120 flows into the liquid feeding catheter assembly 102 through the bubble sensor 115.

[0190] The above method also includes: a cleaning step of the objective lens 104: using a cleaning component 108 connected to the cleaning area 10511 of the working surface 1051 to clean the objective lens 104, the cleaning component 108 includes a cleaning duct component 1081 extending to the cleaning area 10511, and a cleaning power component (not shown in the figure) connected to the cleaning duct component 1081, and the cleaning power component is configured to provide cleaning liquid to the cleaning area 10511 through the cleaning duct component 1081 to clean the objective lens 104 when the objective lens 104 moves above the cleaning area 10511.

[0191] The present application also discloses a method for supplying and discharging liquid for an objective lens 104, wherein the objective lens 104 is used for detecting a platform assembly 105 including a plurality of platforms, wherein the plurality of platforms respectively have working surfaces 1051 and 1052, wherein the method comprises: a liquid supply step: suspending at least two liquid supply conduits 1021 and 1022 above liquid supply areas of the at least two working surfaces 1051 and 1052, respectively; a power assembly 107 sequentially applying working liquid 1120 from a liquid supply station 112 through a fluid interface assembly 106, a conduit structure assembly 101, and the at least two liquid supply conduits 1021 and 1022 to the liquid supply areas on the at least two working surfaces 1051 and 1052; and a detection step: driving the objective lens 104 to move the liquid supply conduits 1021 and 102 ...51 and 1052 to move the liquid supply conduits 1051 and 1052 to move the liquid supply conduits 1051 and 1052 to move the liquid supply conduits 1051 and 1052 to move the liquid supply conduits 1051 and 1052 to move the liquid supply conduits 105 04 moves relative to the at least two working surfaces 1051 and 1052, so that the objective lens 104 successively contacts the working liquid 1120 in the liquid feeding area on the at least two working surfaces 1051 and 1052 and enters a working state, and drives the objective lens 104 to leave the liquid feeding area after the working state is terminated; and a drainage step: after the objective lens 104 leaves the liquid feeding area, at least two drainage conduits 1031 and 1032 are suspended above the liquid feeding area, and the power component 107 extracts the fluid located in the liquid feeding area from the liquid feeding area through the at least two drainage conduits 1031 and 1032, the conduit structure component 101 and the fluid interface component 106 in sequence.

[0192] The at least two liquid supply conduits 1021, 1022 are supported by the same conduit structure assembly 101. During the liquid supply step, the power assembly 107 drives the working liquid 1120 to flow through the conduit structure assembly 101 and simultaneously applied to different working surfaces 1051, 1052 of the at least two working surfaces 1051, 1052 through the at least two liquid supply conduits 1021, 1022.

[0193] In the liquid feeding step, the power assembly 107 sequentially drives the working liquid 1120 to be applied to the corresponding working surface 1051 , 1052 of the at least two liquid feeding conduits 1021 , 1022 .

[0194] The drainage step also includes: using the filter 1063 to filter the waste liquid 1130 converted from the working liquid 1120 and entering the fluid interface component 106 from the liquid supply area into the working liquid 1120, and the power component 107 returns the filtered working liquid 1120 to the liquid supply station 112.

[0195] The draining step further includes: the power component 107 drives the waste liquid 1130 converted from the working liquid 1120 from the liquid supply area into the fluid interface component 106 to flow into the draining station 113 .

[0196] The liquid feeding step also includes a bubble detection step: using the bubble sensor 115 set in the fluid interface assembly 106 to detect whether there are bubbles in the working liquid 1120 to be flowed into the liquid feeding catheter assembly 102 through the bubble sensor 115, so that when bubbles are detected, the working liquid 1120 flows into the liquid discharge station 113 through the bypass line 1064 connected between the bubble sensor 115 and the liquid discharge station 113, and when no bubbles are detected, the working liquid 1120 flows into the liquid feeding catheter assembly 102 through the bubble sensor 115.

[0197] The above method also includes a step of cleaning the objective lens 104: using a cleaning assembly 108 connected to a cleaning area 10511 of the working surfaces 1051 and 1052 to clean the objective lens 104, the cleaning assembly 108 includes a cleaning duct assembly 1081 extending to the cleaning area 10511, and a cleaning power assembly (not shown in the figure) connected to the cleaning duct assembly 1081. The cleaning power assembly is configured to provide cleaning liquid to the cleaning area 10511 through the cleaning duct assembly 1081 to clean the objective lens 104 when the objective lens 104 moves above the cleaning area 10511.

[0198] The present application also discloses a method for supplying and discharging liquid for an objective lens 104, comprising: a) placing a liquid supply conduit assembly 102 in a conduit structure assembly 101 integrally formed with a platform assembly 105, so that the opening of the liquid supply conduit assembly 102 is located in the plane of a working surface 1051 of the platform assembly 105 in the i-th liquid supply area of ​​the working surface 1051 having n liquid supply areas, i = 1, 2, 3 ... n-1, n; and a power assembly 107 sequentially passing a working liquid 1120 from a liquid supply station 112 through a fluid interface assembly 106, a liquid supply conduit assembly 102, and a conduit structure assembly 101. b: placing the drainage conduit assembly 103 in the conduit structure assembly 101 integrally formed with the platform assembly 105, so that the opening of the drainage conduit assembly 103 is located in the j-th drainage area of ​​the working surface 1051 having m drainage areas, j=1, 2, 3...m-1, m, in the plane of the working surface 1051 of the platform assembly 105; c: driving the objective lens 104 to move relative to the working surface 1051, so that the objective lens 104 contacts the working liquid 1120 located in the i-th drainage area and enters the working state. state; d: after the working state is terminated, the objective lens 104 and the waste liquid 1130 converted from the working liquid 1120 attached to the objective lens 104 are driven to leave the i-th liquid supply area; the objective lens 104 and the waste liquid 1130 are moved to just above the opening of the drainage conduit assembly 103, and while the waste liquid 1130 is drawn away from the objective lens 104 and the j-th drainage area by the drainage conduit assembly 103, the liquid supply conduit assembly 102 applies the working liquid 1120 to the i-th liquid supply area of ​​the working surface 1051; e: driving the objective lens 104 relative to the working surface The surface 1051 moves so that the objective lens 104 contacts the working liquid 1120 located in the i-th liquid feeding area and enters a working state; f: looping steps d to e for several times; g: after the working state is terminated, the objective lens 104 and the waste liquid 1130 converted from the working liquid 1120 and attached to the objective lens 104 are driven to leave the i-th liquid feeding area, and the objective lens 104 and the waste liquid 1130 are moved to just above the opening of the drainage duct assembly 103, and the waste liquid 1130 is drawn away from the objective lens 104 and the j-th drainage area by the drainage duct assembly 103.

[0199] In addition, the present application also provides a biochemical detection method assisted by any of the above-mentioned liquid supply and discharge devices, comprising: loading a sample to be detected into a channel of a chip circulation pool; and loading a reagent having a plurality of different reaction components into the channel of the chip circulation pool to perform a biochemical reaction of the sample to be detected and the reagent, wherein the reaction component comprises at least one of a sample generating component and a sample analyzing component; optionally, the biochemical reaction comprises generating a sample in the channel of the chip circulation pool, generating a sample in the channel of the chip circulation pool comprises causing different sample generating components to flow into the channel and controlling the reaction conditions of the channel to generate the sample; and the biochemical reaction comprises analyzing the sample generated in the channel, analyzing the Generating the sample in the channel includes allowing a sample analysis component to flow into the channel, the sample analysis component reacting with the generated sample to provide a relevant detectable signal; applying an immersion medium to a detection position corresponding to the relevant detectable signal on the working surface using a liquid supply conduit assembly; moving the optical system relative to the chip flow cell to the detection position to contact the immersion medium and collect the relevant detectable signal; and separating the optical system from the immersion medium while using a liquid discharge conduit assembly to extract the immersion medium from the detection position; wherein the liquid supply conduit assembly and the liquid discharge conduit assembly are arranged at intervals and the liquid supply conduit assembly and the liquid discharge conduit assembly are interconnected by a conduit structure assembly. The biochemical reaction is a nucleic acid sequencing reaction, and the sample to be detected is a nucleic acid sequencing library. The detectable signal is preferably an optical signal. Alternatively, the sample to be detected can also be a tissue sample, and the biochemical reaction is a specific binding reaction.

[0200] Those skilled in the art will appreciate that the embodiments described above are exemplary and can be improved upon by those skilled in the art. The structures described in various embodiments can be freely combined without causing any conflict in structure or principle.

[0201] After describing the preferred embodiments of the present application in detail, those skilled in the art will clearly understand that various changes and modifications may be made without departing from the scope and spirit of the appended claims, and that the present application is not limited to the implementation methods of the exemplary embodiments given in the specification.

Claims

1. A liquid supply and drainage device for applying working liquid from a liquid supply station to a working surface of a platform assembly, wherein the liquid supply and drainage device is movable relative to the working surface, comprising: a liquid supply conduit assembly for applying a working liquid to the working surface; a drain conduit assembly for withdrawing the working fluid from the working surface; a catheter structure assembly connected between the liquid supply catheter assembly and the liquid discharge catheter assembly; a fluid interface assembly connected between the liquid feeding station and the catheter structure assembly; as well as A power assembly connected to the fluid interface assembly is configured to apply a positive driving force to the liquid supply conduit assembly via the fluid interface assembly to apply the working fluid to the working surface, or to apply a reverse driving force to the liquid discharge conduit assembly to remove the working fluid from the working surface.

2. The liquid supply and discharge device according to claim 1, wherein: The liquid feeding conduit assembly is suspended on one side of the working surface and is opposite to and spaced from the working surface. The liquid draining conduit assembly is spaced from the liquid feeding conduit assembly and suspended on the same side of the working surface.

3. The liquid supply and discharge device according to claim 1, wherein: The conduit structure assembly is formed integrally with the platform assembly so that the openings of the liquid supply conduit assembly and the liquid discharge conduit assembly are located in the plane of the working surface.

4. The liquid supply and discharge device according to claim 2 or 3, wherein: The power assembly includes a driver connected between the liquid feeding station and the fluid interface assembly.

5. The liquid supply and discharge device according to claim 4, wherein: The fluid interface assembly includes a liquid supply line that is fluidically connected to the liquid supply line assembly via the catheter structure assembly, and a liquid discharge line that is fluidically connected to the liquid discharge line assembly via the catheter structure assembly. One end of the liquid supply line and the liquid discharge line that is away from the catheter structure assembly is connected to the driver respectively.

6. The liquid supply and discharge device according to claim 5, wherein: The catheter structure component is provided with a liquid supply interface connecting the liquid supply pipeline and the liquid supply catheter component, and a liquid discharge interface connecting the liquid discharge pipeline and the liquid discharge catheter component.

7. The liquid supply and discharge device according to claim 5, wherein: The power assembly further includes a reversing assembly connected between the driver and one end of the liquid supply line away from the catheter structure assembly and one end of the liquid discharge line away from the catheter structure assembly, the reversing assembly having a first position for connecting the liquid supply line to the driver fluid and disconnecting the liquid discharge line from the driver fluid, and a second position for connecting the liquid discharge line to the driver fluid and disconnecting the liquid supply line from the driver fluid.

8. The liquid supply and discharge device according to claim 7, wherein: The fluid interface assembly further includes a filter located in the drainage pipeline. The fluid received by the drainage pipeline from the drainage conduit assembly is filtered by the filter and then discharged to the liquid feeding station through the reversing assembly.

9. The liquid supply and discharge device according to claim 7, wherein: The reversing component includes at least one of a solenoid valve, a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve, a rotary valve, and a rotary cutting valve.

10. The liquid supply and discharge device according to claim 5 or 6, wherein: It also includes a liquid drainage station, which is connected to the liquid drainage conduit assembly via the driver and the conduit structure assembly.

11. The liquid supply and discharge device according to claim 10, wherein: The driver includes a liquid feeding driver and a liquid discharging driver. The liquid feeding driver is connected between the liquid feeding pipeline and the liquid feeding station, and the liquid discharging driver is connected between the liquid discharging pipeline and the liquid discharging station.

12. The liquid supply and discharge device according to any one of claims 4 to 11, wherein: The driver includes at least one of a peristaltic pump, a plunger pump, a syringe pump, a gear pump, a diaphragm pump, a vacuum pump, a diaphragm pump, and an air compressor.

13. The liquid supply and discharge device according to claim 11, wherein: The liquid supply drive is a first pump, and the liquid discharge drive is a second pump. The first pump and the second pump are connected to form a two-row pump. The two-row pump is configured so that the first pump drives the liquid supply conduit assembly to apply the working liquid to a first position on the working surface, while the second pump drives the liquid discharge conduit assembly to extract fluid located at a second position on the working surface; wherein the first position is opposite to the liquid supply conduit assembly, the second position is opposite to the liquid discharge conduit assembly, and the fluid includes gas located at the second position or waste liquid converted from the working liquid.

14. The liquid supply and discharge device according to claim 11, wherein: The liquid feeding driver is a first pump, and the liquid discharging driver is a second pump, and the first pump and the second pump operate independently of each other.

15. The liquid supply and discharge device according to claim 14, wherein: The first pump and the second pump are respectively located on different sides of the working surface.

16. The liquid supply and drainage device according to any one of claims 11, 13 to 15, wherein: The liquid feeding conduit assembly includes at least two liquid feeding conduits, the liquid feeding pipeline includes liquid feeding sub-pipelines connected one-to-one with the liquid feeding conduits, and a common liquid feeding pipeline connected between the liquid feeding sub-pipelines and the first pump, and a first multi-way connector is connected between the common liquid feeding pipeline and the liquid feeding sub-pipelines.

17. The liquid supply and discharge device according to claim 16, wherein: The drainage conduit assembly includes at least two drainage conduits corresponding one-to-one to the liquid supply conduits, the drainage pipeline includes drainage sub-pipelines connected one-to-one to the drainage conduits, and a common drainage pipeline connected between the drainage sub-pipelines and the second pump, and a second multi-way connector is connected between the common drainage pipeline and the drainage sub-pipelines.

18. The liquid supply and discharge device according to claim 16, wherein: A first two-way valve is provided in at least one of the liquid feeding sub-pipelines.

19. The liquid supply and discharge device according to claim 17, wherein: A second two-way valve is provided in at least one of the liquid discharge sub-pipelines.

20. The liquid supply and discharge device according to claim 11, wherein: The liquid supply pipeline is provided with a sensor, which is configured to detect the quality of the working liquid flowing into the liquid supply pipeline through the driver. The fluid interface assembly also includes a bypass pipeline connected between the sensor and the liquid discharge station. The working liquid detected by the sensor as having quality that does not meet the requirements flows into the liquid discharge station through the bypass pipeline.

21. The liquid supply and discharge device according to claim 20, wherein: The fluid interface assembly further includes a third multi-way connector connecting the liquid discharge station, the liquid discharge driver, and the bypass line.

22. The liquid supply and drainage device according to any one of claims 5 to 9, 11, 13 to 15, and 17 to 21, wherein: It also includes a cleaning station, which at least stores cleaning liquid for regularly cleaning the liquid feeding pipeline.

23. An immersion objective lens system comprising a platform assembly having a working surface, and an objective lens movable relative to the platform assembly, wherein: The immersion objective lens system further comprises a liquid supply and discharge device according to any one of claims 1 to 22, wherein the liquid supply and discharge device provides a working liquid as an immersion medium between the objective lens and the working surface.

24. The immersion objective system of claim 23, wherein: The working surface includes a cleaning area, and the immersion objective system further includes a cleaning assembly connected to the cleaning area; the cleaning assembly includes a cleaning conduit assembly extending to the cleaning area, and a cleaning power assembly connected to the cleaning conduit assembly; The cleaning power assembly is configured to provide cleaning liquid to the cleaning area through the cleaning conduit assembly to clean the objective lens when the objective lens moves above the cleaning area.

25. The immersion objective system of claim 24, wherein: The cleaning conduit assembly includes a first cleaning conduit and a second cleaning conduit. The first cleaning conduit is configured to deliver the cleaning fluid to the cleaning area, and the second cleaning conduit is configured to extract the cleaning fluid from the cleaning area.

26. The immersion objective system of claim 25, wherein: The cleaning duct assembly further includes an air blowing duct, which is used to dry the objective lens after being cleaned by the cleaning liquid.

27. A gene sequencer comprising a gene sequencing chip and an optical system for detecting the gene sequencing chip, wherein: The optical system includes an objective lens capable of moving relative to the gene sequencing chip, and a liquid supply and discharge device for providing an immersion medium between the objective lens and the gene sequencing chip, wherein a side of the gene sequencing chip close to the objective lens is a working surface; The liquid supply and drainage device is used to apply the immersion medium from the liquid supply station to the work surface and is movable relative to the work surface, and the liquid supply and drainage device includes: a liquid feeding conduit assembly suspended on one side of the working surface, facing the working surface and spaced apart; a liquid discharge conduit assembly spaced apart from the liquid supply conduit assembly and suspended on the same side of the working surface; a catheter structure assembly connected between the liquid supply catheter assembly and the liquid discharge catheter assembly; a fluid interface assembly connected between the liquid feeding station and the catheter structure assembly; and a power assembly connected to the fluid interface assembly, the power assembly being configured to apply a positive driving force to the liquid supply conduit assembly via the fluid interface assembly to apply the immersion medium to the work surface, or to apply a negative driving force to the liquid discharge conduit assembly to remove the immersion medium from the work surface.

28. A gene sequencer for multiple chips, the gene sequencer comprising at least two chip detection positions, each of which is used to mount a gene sequencing chip. The gene sequencer also comprises an optical system for detecting the gene sequencing chip, the optical system comprising an objective lens capable of moving relative to the gene sequencing chip, and a liquid supply and discharge device for providing an immersion medium between the objective lens and the gene sequencing chip. The side of each of the at least two gene sequencing chips adjacent to the objective lens serves as a working surface. The liquid supply and drainage device is used to apply the immersion medium from the liquid supply station to the work surface and is movable relative to the work surface, and the liquid supply and drainage device includes: at least two liquid feeding conduits suspended on one side of each of the working surfaces, facing each of the working surfaces and spaced apart; at least two liquid discharge conduits spaced apart from and arranged one-to-one with the at least two liquid supply conduits and suspended on the same side of the working surface; a conduit structure assembly connected between the at least two liquid supply conduits and the at least two liquid discharge conduits; a fluid interface assembly connected between the liquid feeding station and the catheter structure assembly; and a power assembly connected to the fluid interface assembly, the power assembly being configured to apply a positive driving force to the at least two liquid supply conduits via the fluid interface assembly to apply the immersion medium to each of the work surfaces via the at least two liquid supply conduits, or to apply a negative driving force to the at least two liquid discharge conduits to withdraw the immersion medium from each of the work surfaces.

29. A method for supplying and draining liquid for an objective lens, comprising: Liquid feeding step: suspending the liquid feeding conduit assembly above the i-th liquid feeding area on a working surface having n liquid feeding areas, where i=1, 2, 3, ..., n-1, n; and applying working liquid from the liquid feeding station to the i-th liquid feeding area via the fluid interface assembly, the conduit structure assembly, and the liquid feeding conduit assembly in sequence. Detection step: driving the objective lens to move relative to the working surface so that the objective lens contacts the working liquid in the i-th liquid supply area and enters a working state; and driving the objective lens to leave the i-th liquid supply area after the working state is terminated; Drainage step: suspend the drainage conduit assembly above the i-th liquid supply area, and the power assembly extracts the waste liquid converted from the working liquid in the i-th liquid supply area from the i-th liquid supply area through the drainage conduit assembly, the conduit structure assembly and the fluid interface assembly in sequence.

30. The liquid supply and discharge method according to claim 29, wherein: The liquid feeding step, the detection step and the liquid draining step are executed in a cycle until the detection of the nth liquid feeding area by the objective lens is completed and the fluid in the nth liquid feeding area is extracted.

31. The liquid supply and discharge method according to claim 30, wherein: The power component includes a liquid supply driver and a liquid discharge driver. When i≥2, the liquid discharge driver drives the liquid discharge catheter component to perform a liquid discharge step for the i-1 liquid supply area and the liquid supply driver drives the liquid supply catheter component to perform a liquid supply step for the i-th liquid supply area simultaneously.

32. The liquid supply and discharge method according to claim 30, wherein: The fluid includes gas and waste liquid converted from the working liquid, and the drainage step also includes: using the filter in the fluid interface component to filter the waste liquid drawn into the fluid interface component from the i-th liquid supply area and convert it into working liquid, and the power component returns the working liquid to the liquid supply station.

33. The liquid supply and discharge method according to claim 29, wherein: The power assembly includes a common driver, which first drives the drainage conduit assembly to perform the drainage step of the (i-1)th liquid feeding area, and then drives the liquid feeding conduit assembly to perform the liquid feeding step of the (i)th liquid feeding area.

34. The liquid supply and discharge method according to claim 30, wherein: The liquid feeding drive is a first pump, and the liquid discharge drive is a second pump. The first pump and the second pump are connected to each other to form a two-row pump. The two-row pump is configured so that while the first pump drives the liquid feeding catheter assembly to perform the liquid feeding step in one of the liquid feeding areas, the second pump drives the liquid discharge catheter assembly to perform the liquid discharge step in another of the liquid feeding areas.

35. The liquid supply and discharge method according to claim 34, wherein: The second pump is connected between the drainage conduit assembly and the drainage station, and the drainage step further includes the second pump driving the waste liquid from the liquid feeding area into the fluid interface assembly to flow into the drainage station.

36. The liquid supply and discharge method according to claim 35, wherein: The liquid feeding step also includes a bubble detection step: using a bubble sensor provided in the fluid interface assembly to detect whether there are bubbles in the working liquid to be flowed into the liquid feeding conduit assembly via the bubble sensor, so that when bubbles are detected, the working liquid flows into the liquid discharge station via a bypass line connected between the bubble sensor and the liquid discharge station, and when no bubbles are detected, the working liquid flows into the liquid feeding conduit assembly via the bubble sensor.

37. The liquid supply and drainage method according to any one of claims 29 to 36, further comprising: The objective lens cleaning step comprises: cleaning the objective lens using a cleaning assembly connected to the cleaning area of ​​the working surface, the cleaning assembly comprising a cleaning conduit assembly extending to the cleaning area, and a cleaning power assembly connected to the cleaning conduit assembly, the cleaning power assembly being configured to supply cleaning liquid to the cleaning area via the cleaning conduit assembly to clean the objective lens when the objective lens moves above the cleaning area.

38. A method for supplying and draining liquid for an objective lens, wherein the objective lens is used for detecting a platform assembly comprising a plurality of platforms, each of the plurality of platforms having a working surface, wherein: The method comprises: Liquid feeding step: suspending at least two liquid feeding conduit assemblies above the liquid feeding areas of at least two working surfaces respectively, and applying the working liquid from the liquid feeding station to the liquid feeding areas on the at least two working surfaces through the fluid interface assembly, the conduit structure assembly and the at least two liquid feeding conduit assemblies in sequence. a detection step of: driving the objective lens to move relative to the at least two working surfaces so that the objective lens successively contacts the working liquid in the liquid supply areas on the at least two working surfaces and enters a working state, and driving the objective lens to leave the liquid supply area after the working state is terminated; and Liquid drainage step: After the objective lens leaves the liquid feeding area, the liquid drainage catheter assembly is suspended above the liquid feeding area, and the power assembly extracts the fluid located in the liquid feeding area from the liquid feeding area through the liquid drainage catheter assembly, the catheter structure assembly and the fluid interface assembly in sequence.

39. The liquid supply and discharge method according to claim 38, wherein: The at least two liquid feeding conduit assemblies are supported by the same conduit structure assembly. In the liquid feeding step, the power assembly drives the working liquid to flow through the conduit structure assembly and simultaneously applies the working liquid to different working surfaces of the at least two working surfaces through the at least two liquid feeding conduit assemblies.

40. The liquid supply and drainage method according to claim 38, wherein: In the liquid supplying step, the power assembly drives the working liquid in sequence through each of the at least two liquid supplying conduit assemblies to be applied to a corresponding working surface of the at least two working surfaces.

41. The liquid supply and discharge method according to any one of claims 38 to 40, wherein: The liquid discharge step further includes: using a filter to filter the waste liquid converted from the working liquid into the fluid interface component from the liquid supply area into working liquid, and the power component returns the filtered working liquid to the liquid supply station.

42. The liquid supply and discharge method according to any one of claims 38 to 40, wherein: The draining step further includes: the power component driving the waste liquid converted from the working liquid from the liquid supply area into the fluid interface component to flow into the draining station.

43. The liquid supply and discharge method according to claim 42, wherein: The liquid feeding step also includes a bubble detection step: using a bubble sensor provided in the fluid interface assembly to detect whether there are bubbles in the working liquid to be flowed into the liquid feeding conduit assembly via the bubble sensor, so that when bubbles are detected, the working liquid flows into the liquid discharge station via a bypass line connected between the bubble sensor and the liquid discharge station, and when no bubbles are detected, the working liquid flows into the liquid feeding conduit assembly via the bubble sensor.

44. The liquid supply and discharge method according to any one of claims 38 to 40, further comprising an objective lens cleaning step: the objective lens is cleaned using a cleaning assembly connected to the cleaning area of ​​the working surface, the cleaning assembly comprising a cleaning duct assembly extending to the cleaning area, and a cleaning power assembly connected to the cleaning duct assembly, the cleaning power assembly being configured to provide cleaning liquid to the cleaning area via the cleaning duct assembly to clean the objective lens when the objective lens moves above the cleaning area.

45. A method for feeding and draining liquid for an objective lens, comprising: a. Placing the liquid feeding conduit assembly within a conduit structure assembly integrally formed with the platform assembly, such that an opening of the liquid feeding conduit assembly is located within the plane of a working surface of the platform assembly within an i-th liquid feeding area of ​​the working surface having n liquid feeding areas, where i = 1, 2, 3 ... n-1, n; and applying working liquid from the liquid feeding station to the i-th liquid feeding area via the fluid interface assembly, the liquid feeding conduit assembly, and the conduit structure assembly in sequence. b: placing the drainage conduit assembly within a conduit structure assembly integrally formed with the platform assembly, such that an opening of the drainage conduit assembly is located within a plane of a working surface of the platform assembly in a j-th drainage area of ​​the working surface having m drainage areas, where j = 1, 2, 3 ... m-1, m; c: driving the objective lens to move relative to the working surface so that the objective lens contacts the working liquid in the i-th liquid feeding area and enters a working state; d. After the working state is terminated, the objective lens and the waste liquid converted from the working liquid and attached to the objective lens are driven to leave the i-th liquid supply area; the objective lens and the waste liquid are moved to just above the opening of the drainage conduit assembly, and while the waste liquid is drawn away from the objective lens and the j-th drainage area by the drainage conduit assembly, the liquid supply conduit assembly applies the working liquid to the i-th liquid supply area of ​​the working surface; e: driving the objective lens to move relative to the working surface so that the objective lens contacts the working liquid in the i-th liquid feeding area and enters a working state; f: Loop through steps d to e for several times; g: After the working state is terminated, the objective lens and the waste liquid converted from the working liquid attached to the objective lens are driven to leave the i-th liquid supply area, and the objective lens and the waste liquid are moved to just above the opening of the drainage duct assembly, and the waste liquid is drawn away from the objective lens and the j-th drainage area by the drainage duct assembly.

46. ​​A biochemical detection method comprising: Load the sample to be tested into the channel of the chip flow cell; as well as loading reagents having a plurality of different reaction components into the channels of the chip flow cell to perform a biochemical reaction between the sample to be detected and the reagents, wherein the reaction components include at least one of a sample generation component and a sample analysis component; Optionally, the biochemical reaction comprises generating a sample in a channel of the chip flow cell, and generating the sample in the channel of the chip flow cell comprises flowing different sample generating components into the channel and controlling reaction conditions of the channel to generate the sample; and The biochemical reaction comprises analyzing the generated sample in the channel, wherein analyzing the generated sample in the channel comprises flowing a sample analysis component into the channel, wherein the sample analysis component reacts with the generated sample to provide a related detectable signal; applying an immersion medium to a detection location on the working surface corresponding to the associated detectable signal using a liquid delivery conduit assembly; moving the optical system relative to the chip flow cell to the detection position to contact the immersion medium and collect the associated detectable signal; and separating the optical system from the immersion medium and simultaneously pumping the immersion medium away from the detection location using a drainage conduit assembly; The liquid feeding conduit assembly and the liquid draining conduit assembly are spaced apart and are connected to each other via a conduit structure assembly.

47. The biochemical test method according to claim 46, characterized in that The biochemical reaction is a nucleic acid sequencing reaction, and the sample to be detected is a nucleic acid sequencing library.

48. The biochemical testing method according to claim 46, characterized in that The detectable signal is an optical signal.

49. The biochemical test method according to claim 46, characterized in that The sample to be detected is a tissue sample, and the biochemical reaction is a specific binding reaction.

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