Device and method capable of preventing predatory mites from escaping based on toxicity quantitative test

Abstract

The invention relates to the technical field of chemical analysis, in particular to a toxicity quantitative test-based device and method capable of preventing predatory mites from escaping, and the device comprises a glass plate which is installed at the bottom of the toxicity quantitative test-based device capable of preventing predatory mites from escaping, is connected with a PVC plastic plate, and is used for placing a compound to be tested; the PVC plastic plate is mounted on the glass plate, connected with the nylon gauze element and used for fixing the nylon gauze element; the rectangular groove is formed in the PVC plastic plate and used for containing the predatory mites, and the nylon gauze element is connected with the PVC plastic plate and used for sealing the device capable of preventing the predatory mites from escaping based on the toxicity quantitative test. According to the invention, the accuracy of the quantitative test on the toxicity of the predatory mites is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of chemical analysis, and in particular to a device and method for preventing predatory mites from escaping based on toxicity quantitative testing. Background Art

[0002] The natural enemies of predatory mites are invertebrates belonging to the phylum Arthropoda, class Arachnida. Predatory mites are natural enemies of many harmful mites and are a very important type of non-target arthropod. Using them to control agricultural pest mites is of great value and practical significance in agricultural production. However, in my country's current agricultural production, there are many types of diseases and insect pests, and currently they mainly rely on pesticides for prevention and control. However, while pesticides kill target pests in the field, they also have a serious impact on natural enemies such as predatory mites, especially the use of broad-spectrum insecticides such as organophosphorus and pyrethroids, which have great toxic effects on predatory mites. For this reason, it is very important to conduct relevant acute toxicity tests on predatory mites. The toxicity results obtained can reasonably and scientifically guide agricultural practices and effectively control the use of pesticides.

[0003] Chinese patent publication number: CN114878761B discloses a method and application of using Daphnia magna to quickly screen organophosphorus pesticide residues in strawberries. Daphnia magna is used as a toxicity test organism. By measuring the 3-hour mortality of Daphnia magna under different concentrations of 8 common organophosphorus pesticide residues such as dichlorvos, parathion, phoxim, methyl parathion, malathion, omethoate, methyl isofenphos, and trichlorfon, the minimum total lethal concentration of each organophosphorus pesticide is found. Combined with the weighing amount and dilution multiple of the strawberry sample to be tested, the detection limit of the 8 organophosphorus pesticide residues is calculated to quickly screen the excessive organophosphorus pesticide residues in strawberries. However, this scheme does not limit the escape of Daphnia magna, and it is difficult to improve the accuracy of toxicity quantitative testing. Summary of the invention

[0004] To this end, the present invention provides a device and method for preventing predatory mites from escaping based on toxicity quantitative testing, so as to overcome the problem of low accuracy of toxicity quantitative testing in the prior art.

[0005] To achieve the above object, the present invention provides a device for preventing predatory mites from escaping based on toxicity quantitative testing, the device comprising: A glass plate, which is installed at the bottom of the device for preventing predatory mites from escaping based on toxicity quantitative testing, connected to a PVC plastic plate, and used for placing the test compound and the predatory mites; A PVC plastic plate is mounted on the glass plate and connected to the nylon mesh to fix the nylon mesh; Rectangular troughs, mounted on PVC plastic boards, are used to place predatory mites. The nylon gauze is connected to the PVC plastic plate and is used to seal the device capable of preventing the predatory mite from escaping based on the toxicity quantitative test.

[0006] Furthermore, in the glass plate, the length of the glass plate is set to A, A=60 mm, the width of the glass plate is set to B, B=50 mm, and the thickness of the glass plate is set to C, 1 mm≤C≤1.5 mm.

[0007] Furthermore, in the PVC plastic plate, it is set that the outer ring length of the PVC plastic plate is A2=50 mm, and the outer ring width of the PVC plastic plate is B2=40 mm.

[0008] Furthermore, in the rectangular groove, the length of the rectangular groove is set to K2, and the width of the rectangular groove is set to L2, wherein K2=40mm, and L2=30mm.

[0009] Furthermore, in the nylon gauze, the color of the nylon gauze is set to black, the gap diameter d=0.4 μm, the length of the nylon gauze is set to K1=40 mm, and the width of the nylon gauze is set to L1=30 mm.

[0010] On the other hand, the present invention also provides a method for preventing predatory mites from escaping based on toxicity quantitative testing, characterized in that the method comprises: Step S1, spraying the compound to be tested onto the glass plate, and waiting for the compound to be tested to dry, to obtain a dry medicine glass plate; Step S2, cutting the rectangular groove, and connecting the PVC plastic plate with the nylon mesh to obtain a plastic plate with a mesh; Step S3, connecting the plastic plate with the net to the glass plate to obtain the device for preventing the predatory mite from escaping based on the toxicity quantitative test described in this scheme; Step S4, putting the predatory mite into a paralyzed state; Step S5, placing the predatory mite in a rectangular groove of the device for preventing the predatory mite from escaping based on toxicity quantitative testing described in this scheme, and placing the device in an artificial incubator; Step S6, observing and recording the initial number of predatory mites in the rectangular slot and the number of dead predatory mites corresponding to each observation and recording time, and calculating the toxicity test result.

[0011] Furthermore, in step S1, a POTTER spray tower is used to evenly spray the test compound on the glass plate, and the test compound dosage is set to J, 1.8 mg / cm 2 ≤J≤2.2mg / cm 2 And wait for the compound to be tested to dry to obtain a dry reagent glass plate.

[0012] Further, in the step S2, when cutting the rectangular groove and connecting the PVC plastic board with the nylon gauze, a fine brush knife is used to cut the rectangular groove on the PVC plastic board, and the nylon gauze is covered on the PVC plastic board to obtain a plastic board with a mesh, wherein the length of the rectangular groove is K2, and the width of the rectangular groove is L2, wherein K2=40 mm, L2=30 mm; In the step S3, a plastic plate with a net is covered on a glass plate to obtain a device for preventing predatory mites from escaping based on toxicity quantitative testing as described in the present scheme; In step S4, the predatory mite is placed in a paralysis device, and the paralysis time is set to t, t=2 min.

[0013] Further, in the step S5, a No. 0 wolf hair brush is used to pick a predatory mite at the center of the glass plate; In step S5, the device is placed in an artificial incubator, with the glass plate facing downward and the nylon mesh facing upward. The parameters of the artificial incubator are: the light-dark ratio is U, U=14h:10h, the incubation temperature is R, 24°C≤R≤26°C, and the incubation humidity is H, 75%≤H≤85%.

[0014] Further, in step S6, when observing and recording the initial number of predatory mites in the rectangular slot and the number of predatory mites that died at each observation and recording time, and calculating the toxicity test result, the observation and recording time intervals are set to be G1, G2 and G3, the initial number of predatory mites is P0, the number of predatory mites that died at each observation and recording time is Pd1, Pd2 and Pd3, and the toxicity test result is X, where: G1=24h; G2=48h; G3=72h; X=[Pd1 / P0+(P0-Pd1) / (Pd2-Pd1)+(P0-Pd1-Pd2) / (Pd3-Pd2-Pd1)] / 3×100%.

[0015] Compared with the prior art, the beneficial effect of the present invention lies in that the device is applied to the process of quantitative testing of pesticide toxicity to predatory mites and quantitative testing of predatory mites for risk assessment, the device realizes effective fixation of predatory mites and precise control of toxicity testing by setting glass plates, PVC plastic plates, rectangular grooves and nylon gauze, in particular, the device uses the glass plate as the bottom of the device to ensure the stability of the entire device and ensure sufficient contact between the agent and the predatory mites, thereby improving the accuracy of quantitative testing of predatory mites toxicity, the device realizes precise connection of the device through the PVC plastic plate, effectively preventing the escape of predatory mites, thereby further improving the accuracy of quantitative testing of predatory mites toxicity, the device provides a fixed activity space for predatory mites through the rectangular groove, which helps to reduce the movement and escape of predatory mites during the process of quantitative testing of predatory mites toxicity, so as to improve the accuracy of quantitative testing of predatory mites toxicity, the device ensures sufficient air circulation and light inside the device through the nylon gauze to meet the survival needs of predatory mites, so as to simulate the real living environment of predatory mites, thereby further improving the accuracy of quantitative testing of predatory mites toxicity.

[0016] In particular, the glass plate serves as the bottom of the device, and its solid structure and stable size can ensure the stability of the entire device, preventing the accuracy of the quantitative test of predatory mite toxicity from being affected by shaking and deformation of the device during the quantitative test of predatory mite toxicity.

[0017] In particular, in the PVC plastic plate, the size of the PVC plastic plate is designed to be closely attached to the glass plate to form a stable structure, which helps to prevent the predatory mites from escaping and can also ensure sufficient contact between the test compound and the predatory mites.

[0018] In particular, the rectangular groove provides activity space for predatory mites, which helps to reduce the stress response of predatory mites during the quantitative test of predatory mite toxicity and improve the accuracy and reliability of the quantitative test of predatory mite toxicity.

[0019] In particular, in the nylon gauze, the gap diameter d of the nylon gauze is set to be much smaller than 0.4 μm, which is the body diameter of the predatory mite. This can effectively prevent the predatory mites from escaping through the gauze during the quantitative test of the toxicity of predatory mites, thereby ensuring the closure of the quantitative test of the toxicity of predatory mites and the accuracy of the results.

[0020] In particular, the method is applied to a device capable of preventing predatory mites from escaping based on toxicity quantitative testing. The method realizes precise control of the predatory mite toxicity quantitative testing through steps S1 to S6, effectively preventing the predatory mites from escaping during the process of the predatory mite toxicity quantitative testing, and can also ensure that the test compound is in full contact with the predatory mites, thereby improving the accuracy and reliability of the predatory mite toxicity quantitative testing. In particular, the method sprays the test compound onto the glass plate through step S1 to ensure uniform distribution of the agent and full contact with the predatory mites, and waits for the test compound to dry to form a A layer of stable agent film is provided to facilitate the subsequent observation and recording of the quantitative test of the toxicity of predatory mites. The method cuts a rectangular groove through step S2, and connects the PVC plastic plate with the nylon mesh to form a closed test space, which helps to prevent the escape of predatory mites during the quantitative test of the toxicity of predatory mites, while ensuring the suitability of air and humidity. The method connects the plastic plate with a mesh with the glass plate through step S3 to obtain the device for preventing the escape of predatory mites based on the quantitative test of toxicity described in this scheme, realizes a controllable experimental environment, and ensures the accuracy and Repeatability. The method causes the predatory mite to enter a paralyzed state through step S4, slowing down its metabolic rate and activity ability, thereby facilitating the subsequent experimental operation of the quantitative test of the toxicity of the predatory mite, helping to reduce the stress response and escape risk of the predatory mite during the quantitative test of the toxicity of the predatory mite, and improving the accuracy and reliability of the quantitative test of the toxicity of the predatory mite. The method places the predatory mite in the rectangular groove of the device for preventing the predatory mite from escaping based on the toxicity quantitative test described in this scheme through step S5, and puts the device into an artificial incubator to ensure direct contact between the predatory mite and the agent. The method helps to accurately evaluate the toxic reaction of the test compound on the predatory mite and improve the accuracy of the quantitative toxicity test of the predatory mite. The method observes and records the initial number of predatory mites in the rectangular groove and the number of deaths of predatory mites at each observation and recording time through step S6, and calculates the toxicity test results. The toxic effect of the agent on the predatory mites can be accurately evaluated. At the same time, according to the calculation of the initial number and the number of deaths, the toxicity test results can be obtained, which provides data support for subsequent scientific research and analysis, optimizes the method and conditions for the quantitative toxicity test of the predatory mite, and improves the accuracy and reliability of the quantitative toxicity test of the predatory mite.

[0021] In particular, in step S1, the POTTER spray tower can ensure that the agent is sprayed onto the glass plate in a uniform and stable manner, which helps to ensure that each predatory mite is exposed to the same dose of the agent, thereby improving the accuracy and reliability of the quantitative test of predatory mite toxicity.

[0022] In particular, in step S2, the size of the rectangular groove is precisely controlled by a fine-brush knife, which helps to reduce experimental errors and improve the accuracy and reliability of quantitative testing of predatory mite toxicity.

[0023] In particular, in step S3, a closed and breathable environment is formed, which effectively prevents predatory mites from escaping from the device and ensures the stability of the experimental environment. At the same time, it does not affect the ventilation and light exposure inside the device, thereby providing more reliable and consistent experimental conditions and improving experimental efficiency and data accuracy.

[0024] In particular, in step S4, by setting a specific paralysis time, the metabolic rate and activity of the predatory mite can be reduced, thereby reducing the stress response and escape risk during the quantitative test of the toxicity of the predatory mite.

[0025] In particular, in step S5, the No. 0 wolf hair brush can accurately pick up and place the predatory mites, ensuring that the predatory mites are placed in the center of the glass plate, thereby reducing experimental errors caused by position deviations. The artificial incubator provides a stable and controllable experimental environment, including precise light-dark ratios, temperature and humidity, which helps to reduce environmental variations in the process of quantitative testing of predatory mite toxicity and ensure the accuracy and reliability of the results of quantitative testing of predatory mite toxicity.

[0026] In particular, in step S6, by setting the observation and recording time interval, the initial number of predatory mites and the number of deaths of predatory mites at each observation and recording time, and adopting a toxicity test result calculation method, it helps to reduce experimental errors and improve the accuracy and reliability of the quantitative test results of predatory mites toxicity. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the structure of a device for preventing predatory mites from escaping based on toxicity quantitative testing in this embodiment; Figure 2 Schematic diagram of the process of the method for preventing predatory mites from escaping based on toxicity quantitative testing in this embodiment. DETAILED DESCRIPTION

[0028] In order to make the objects and advantages of the present invention more clearly understood, the present invention is further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0029] The preferred embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principles of the present invention and are not intended to limit the protection scope of the present invention.

[0030] It should be noted that, in the description of the present invention, terms such as "up", "down", "left", "right", "inside" and "outside" indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings. This is merely for the convenience of description and does not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present invention.

[0031] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0032] See also Figure 1 As shown, it is a schematic diagram of the structure of a device for preventing predatory mites from escaping based on toxicity quantitative testing in this embodiment, and the device includes: A glass plate 1, which is installed at the bottom of the device for preventing predatory mites from escaping based on toxicity quantitative testing, is connected to a PVC plastic plate 2 and is used to place the compound to be tested; A PVC plastic plate 2 is mounted on the glass plate 1 and connected to the nylon mesh 3 to fix the nylon mesh 3; The rectangular groove 201 is mounted on the PVC plastic plate 2 and is used to place the predatory mites. The nylon gauze 3 is connected to the PVC plastic plate and is used to seal the device for preventing the predatory mite from escaping based on the toxicity quantitative test.

[0033] Specifically, the device is used in the process of quantitative testing and risk assessment of pesticide toxicity to predatory mites. The device realizes effective fixation of predatory mites and precise control of toxicity testing by setting a glass plate, a PVC plastic plate, a rectangular groove and a nylon gauze. In particular, the device uses a glass plate as the bottom of the device to ensure the stability of the entire device and ensure sufficient contact between the agent and the predatory mites, thereby improving the accuracy of the quantitative testing of the toxicity of predatory mites. The device realizes precise connection of the device through the PVC plastic plate, effectively preventing the escape of the predatory mites, thereby further improving the accuracy of the quantitative testing of the toxicity of predatory mites. The device provides a fixed activity space for predatory mites through the rectangular groove, which helps to reduce the movement and escape of predatory mites during the process of quantitative testing of the toxicity of predatory mites, so as to improve the accuracy of the quantitative testing of the toxicity of predatory mites. The device ensures sufficient air circulation and light inside the device through the nylon gauze to meet the survival needs of predatory mites, so as to simulate the real living environment of predatory mites, thereby further improving the accuracy of the quantitative testing of the toxicity of predatory mites.

[0034] Specifically, in the glass plate 1, the length of the glass plate 1 is set to A, A=60 mm, the width of the glass plate 1 is set to B, B=50 mm, and the thickness of the glass plate 1 is set to C, 1 mm≤C≤1.5 mm.

[0035] Specifically, the length A of the glass plate 1 refers to the straight-line distance of the glass plate 1 along its longest direction, the width B of the glass plate 1 refers to the straight-line distance of the glass plate 1 along a direction perpendicular to the length A of the glass plate 1, and the thickness C of the glass plate refers to the perpendicular distance from one surface of the glass plate 1 to its opposite surface.

[0036] Specifically, in the glass plate, the glass plate serves as the bottom of the device, and its solid structure and stable size can ensure the stability of the entire device, preventing the accuracy of the quantitative test of predatory mite toxicity from being affected by shaking and deformation of the device during the quantitative test of predatory mite toxicity.

[0037] Specifically, in the PVC plastic plate 2, it is set that the outer ring length of the PVC plastic plate 2 is A2=50 mm, and the outer ring width of the PVC plastic plate 2 is B2=40 mm.

[0038] Specifically, the outer ring length A2 of the PVC plastic plate 2 refers to the straight-line distance of the PVC plastic plate 2 along its longest outer edge, and the outer ring width B2 of the PVC plastic plate 2 refers to the straight-line distance of the outer edge of the PVC plastic plate 2 along a direction perpendicular to the outer ring length A2 of the PVC plastic plate 2.

[0039] Specifically, in the PVC plastic plate, the size of the PVC plastic plate is designed to fit tightly on the glass plate to form a stable structure, which helps to prevent the predatory mites from escaping and can also ensure sufficient contact between the test compound and the predatory mites.

[0040] Specifically, in the rectangular groove 201, the length of the rectangular groove 201 is set to K2, and the width of the rectangular groove 201 is set to L2, wherein K2=40 mm, and L2=30 mm.

[0041] Specifically, the length K2 of the rectangular groove 201 refers to the straight-line distance of the rectangular groove 201 along its longest side, and the width L2 of the rectangular groove 201 refers to the straight-line distance of the side of the rectangular groove 201 perpendicular to the length K2 of the rectangular groove 201 .

[0042] Specifically, the rectangular groove provides activity space for predatory mites, which helps to reduce the stress response of predatory mites during the quantitative test of predatory mite toxicity and improve the accuracy and reliability of the quantitative test of predatory mite toxicity.

[0043] Specifically, in the nylon mesh 3, the color of the nylon mesh 3 is set to black, the gap diameter d=0.4 μm, the length of the nylon mesh 3 is K1=40 mm, and the width of the nylon mesh 3 is L1=30 mm.

[0044] Specifically, the gap diameter d refers to the diameter of a single gap in the nylon mesh 3, the length K1 of the nylon mesh 3 refers to the straight-line distance of the nylon mesh along its longest side, and the width L1 of the nylon mesh 3 refers to the straight-line distance of the nylon mesh along the side perpendicular to the length K1 of the nylon mesh 3.

[0045] Specifically, in the nylon gauze, the gap diameter d of the nylon gauze is set to be much smaller than 0.4 μm, which is the body diameter of the predatory mite. This can effectively prevent the predatory mites from escaping through the gauze during the quantitative test of the toxicity of predatory mites, thereby ensuring the closure of the quantitative test of the toxicity of predatory mites and the accuracy of the results.

[0046] See also Figure 2 As shown, it is a schematic flow chart of a method for preventing predatory mites from escaping based on toxicity quantitative testing in this embodiment, the method comprising: Step S1, spraying the compound to be tested onto the glass plate, and waiting for the compound to be tested to dry, to obtain a dry medicine glass plate; Step S2, cutting the rectangular groove, and connecting the PVC plastic plate with the nylon mesh to obtain a plastic plate with a mesh; Step S3, connecting the plastic plate with the net to the glass plate to obtain the device for preventing the predatory mite from escaping based on the toxicity quantitative test described in this scheme; Step S4, putting the predatory mite into a paralyzed state; Step S5, placing the predatory mite in a rectangular groove of the device for preventing the predatory mite from escaping based on toxicity quantitative testing described in this scheme, and placing the device in an artificial incubator; Step S6, observing and recording the initial number of predatory mites in the rectangular slot and the number of dead predatory mites corresponding to each observation and recording time, and calculating the toxicity test result.

[0047] Specifically, the method is applied to a device capable of preventing predatory mites from escaping based on a toxicity quantitative test. The method achieves precise control of the predatory mite toxicity quantitative test through steps S1 to S6, effectively preventing the predatory mites from escaping during the process of the predatory mite toxicity quantitative test. The method can also ensure sufficient contact between the test compound and the predatory mites, thereby improving the accuracy and reliability of the predatory mite toxicity quantitative test. In particular, the method is applied to a device capable of preventing predatory mites from escaping based on a toxicity quantitative test. The method achieves precise control of the predatory mite toxicity quantitative test through steps S1 to S6, effectively preventing the predatory mites from escaping during the process of the predatory mite toxicity quantitative test. It can also ensure that the test compound is in full contact with the predatory mite, thereby improving the accuracy and reliability of the quantitative test of the toxicity of the predatory mite. In particular, the method sprays the test compound onto the glass plate through step S1 to ensure the uniform distribution of the agent and full contact with the predatory mite, and waits for the test compound to dry to form a layer of stable agent film, which is convenient for the subsequent observation and recording of the quantitative test of the toxicity of the predatory mite. The method intercepts the rectangular groove through step S2 and connects the PVC plastic plate with the nylon mesh to form a closed test space, which helps to prevent the escape of the predatory mite during the quantitative test of the toxicity of the predatory mite, while ensuring the suitability of the air and humidity. The method will The plastic plate with a net is connected to the glass plate to obtain the device for preventing the escape of predatory mites based on the quantitative toxicity test described in the present invention, so as to realize a controllable experimental environment and ensure the accuracy and repeatability of the quantitative toxicity test of predatory mites. The method causes the predatory mites to enter a paralyzed state through step S4, slowing down their metabolic rate and activity, thereby facilitating subsequent experimental operations for the quantitative toxicity test of predatory mites, helping to reduce the stress response and escape risk of predatory mites during the quantitative toxicity test of predatory mites, and improving the accuracy and reliability of the quantitative toxicity test of predatory mites. The method places the predatory mites into the device for preventing the escape of predatory mites based on the quantitative toxicity test described in the present invention through step S5. The method comprises the following steps: placing the predatory mites in a rectangular groove and placing the device in an artificial incubator to ensure direct contact between the predatory mites and the agent, which is helpful to accurately evaluate the toxic response of the test compound to the predatory mites and improve the accuracy of the quantitative toxicity test of the predatory mites. The method comprises the following steps: observing and recording the initial number of predatory mites in the rectangular groove and the number of deaths of predatory mites at each observation and recording time, and calculating the toxicity test results. The toxicity effect of the agent on the predatory mites can be accurately evaluated. At the same time, the toxicity test results can be obtained based on the calculation of the initial number and the number of deaths, so as to provide data support for subsequent scientific research and analysis, optimize the method and conditions for the quantitative toxicity test of the predatory mites, and improve the accuracy and reliability of the quantitative toxicity test of the predatory mites.

[0048] Specifically, in step S1, a POTTER spray tower is used to evenly spray the test compound on the glass plate, and the dose of the test compound is set to J, 1.8 mg / cm2 ≤J≤2.2mg / cm 2 And wait for the compound to be tested to dry to obtain a dry reagent glass plate.

[0049] Specifically, the test compound refers to a chemical substance whose effect on predatory mites needs to be evaluated in a quantitative test of toxicity to predatory mites, such as insecticides and miticides, the POTTER spray tower refers to a laboratory device used to evenly distribute liquid on a glass plate by precision spraying, and the test compound dose J refers to the mass of the agent sprayed on a unit area of ​​the glass plate.

[0050] Specifically, in step S1, the POTTER spray tower can ensure that the agent is sprayed onto the glass plate in a uniform and stable manner, which helps to ensure that each predatory mite is exposed to the same dose of the agent, thereby improving the accuracy and reliability of the quantitative test of predatory mite toxicity.

[0051] Specifically, in step S2, when cutting the rectangular groove and connecting the PVC plastic board with the nylon mesh, a fine brush knife is used to cut the rectangular groove on the PVC plastic board, and the nylon mesh is covered on the PVC plastic board to obtain a plastic board with a mesh, wherein the length of the rectangular groove is K2, and the width of the rectangular groove is L2, wherein K2=40mm, L2=30mm.

[0052] Specifically, in step S2, the size of the rectangular groove is precisely controlled by a fine-brush knife, which helps to reduce experimental errors and improve the accuracy and reliability of quantitative testing of predatory mite toxicity.

[0053] Specifically, in step S3, a plastic plate with a mesh is covered on a glass plate to obtain a device for preventing predatory mites from escaping based on toxicity quantitative testing as described in this scheme.

[0054] Specifically, in step S3, a closed and breathable environment is formed to effectively prevent predatory mites from escaping from the device, ensuring the stability of the experimental environment, while not affecting the ventilation and light exposure inside the device, thereby providing more reliable and consistent experimental conditions and improving experimental efficiency and data accuracy.

[0055] Specifically, in step S4, the predatory mite is placed in a paralysis device, and the paralysis time is t, t=2 min.

[0056] Specifically, the paralysis device refers to a device used to put predatory mites into a paralysis state. This embodiment does not limit the type of the paralysis device. Relevant technical personnel in this field can freely set it according to actual needs. It only needs to meet the need to put predatory mites into a paralysis state. For example, the paralysis device can be set as a disposable lunch box with cotton balls soaked in ether in advance.

[0057] Specifically, in step S4, by setting a specific paralysis time, the metabolic rate and activity of the predatory mite can be reduced, thereby reducing the stress response and escape risk during the quantitative test of the toxicity of the predatory mite.

[0058] Specifically, in step S5, a No. 0 wolf hair brush is used to pick a predatory mite at the center of the glass plate; In step S5, the device is placed in an artificial incubator, with the glass plate facing downward and the nylon mesh facing upward. The parameters of the artificial incubator are: the light-dark ratio is U, U=14h:10h, the incubation temperature is R, 24°C≤R≤26°C, and the incubation humidity is H, 75%≤H≤85%.

[0059] Specifically, the No. 0 wolf hair brush refers to a laboratory equipment used for conducting quantitative toxicity tests. This embodiment does not limit the type of the No. 0 wolf hair brush. Relevant technical personnel in this field can freely set it according to actual needs. It only needs to meet the requirement of picking predatory mites at the center of the glass plate. For example, the type of the No. 0 wolf hair brush can be set to be a laboratory equipment made of wolf hair for conducting quantitative toxicity tests. The artificial incubator refers to a laboratory equipment used to provide stable environmental conditions. The light-dark ratio U refers to the ratio of the light time to the dark time. The incubation temperature R refers to the temperature set in the parameters of the artificial incubator. The incubation humidity H refers to the humidity set in the parameters of the artificial incubator.

[0060] Specifically, in step S5, the No. 0 wolf hair brush can accurately pick up and place the predatory mites, ensuring that the predatory mites are placed in the center of the glass plate, thereby reducing experimental errors caused by position deviations. The artificial incubator provides a stable and controllable experimental environment, including precise light-dark ratios, temperature and humidity, which helps to reduce environmental variations in the process of quantitative testing of predatory mite toxicity and ensure the accuracy and reliability of the results of quantitative testing of predatory mite toxicity.

[0061] Specifically, in step S6, when observing and recording the initial number of predatory mites in the rectangular slot and the number of predatory mites that died at each observation and recording time, and calculating the toxicity test result, the observation and recording time intervals are set to be G1, G2, and G3, the initial number of predatory mites is P0, the number of predatory mites that died at each observation and recording time is Pd1, Pd2, and Pd3, and the toxicity test result is X, where: G1=24h; G2=48h; G3=72h; X=[Pd1 / P0+(P0-Pd1) / (Pd2-Pd1)+(P0-Pd1-Pd2) / (Pd3-Pd2-Pd1)] / 3×100%.

[0062] Specifically, the observation and recording time interval refers to the observation and recording time difference set during the toxicity test, the initial number of predatory mites refers to the total number of predatory mites placed in the rectangular groove before the start of the toxicity test, and the death number of predatory mites at each observation and recording time refers to the death number of predatory mites recorded at the end of each observation and recording time interval.

[0063] Specifically, in step S6, by setting the observation and recording time interval, the initial number of predatory mites and the number of deaths of predatory mites at each observation and recording time, and adopting a toxicity test result calculation method, it helps to reduce experimental errors and improve the accuracy and reliability of the quantitative test results of predatory mites toxicity.

[0064] So far, the technical solutions of the present invention have been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. A device for preventing predatory mites from escaping based on toxicity quantitative testing, characterized in that: The device comprises: A glass plate, which is installed at the bottom of the device for preventing predatory mites from escaping based on toxicity quantitative testing, connected to a PVC plastic plate, and is used to place the compound to be tested; A PVC plastic plate is mounted on the glass plate and connected to the nylon mesh to fix the nylon mesh; Rectangular troughs, mounted on PVC plastic boards, are used to place predatory mites. The nylon gauze is connected to the PVC plastic plate and is used to seal the device capable of preventing the predatory mite from escaping based on the toxicity quantitative test.

2. The device for preventing predatory mites from escaping based on toxicity quantitative testing according to claim 1, characterized in that: In the glass plate, the length of the glass plate is set to A, A=60 mm, the width of the glass plate is set to B, B=50 mm, and the thickness of the glass plate is set to C, 1 mm≤C≤1.5 mm.

3. The device for preventing predatory mites from escaping based on toxicity quantitative testing according to claim 1, characterized in that: In the PVC plastic plate, it is set that the outer ring length of the PVC plastic plate is A2=50mm, and the outer ring width of the PVC plastic plate is B2=40mm.

4. The device for preventing predatory mites from escaping based on toxicity quantitative testing according to claim 1, characterized in that: In the rectangular groove, the length of the rectangular groove is set to K2, and the width of the rectangular groove is set to L2, wherein K2=40mm, and L2=30mm.

5. The device for preventing predatory mites from escaping based on toxicity quantitative testing according to claim 1, characterized in that: In the nylon mesh, the color of the nylon mesh is set to black, the gap diameter d=0.4 μm, the length of the nylon mesh is K1=40 mm, and the width of the nylon mesh is L1=30 mm.

6. A device for preventing predatory mites from escaping based on toxicity quantitative testing as described in any one of claims 1 to 5, characterized in that: The method comprises: Step S1, spraying the compound to be tested onto the glass plate, and waiting for the compound to be tested to dry, to obtain a dry medicine glass plate; Step S2, cutting the rectangular groove, and connecting the PVC plastic plate with the nylon mesh to obtain a plastic plate with a mesh; Step S3, connecting the plastic plate with the net to the glass plate to obtain the device for preventing the predatory mite from escaping based on the toxicity quantitative test described in this scheme; Step S4, putting the predatory mite into a paralyzed state; Step S5, placing the predatory mite in a rectangular groove of the device for preventing the predatory mite from escaping based on toxicity quantitative testing described in this scheme, and placing the device in an artificial incubator; Step S6, observing and recording the initial number of predatory mites in the rectangular slot and the number of dead predatory mites corresponding to each observation and recording time, and calculating the toxicity test result.

7. The method for preventing predatory mites from escaping based on toxicity quantitative testing according to claim 6, characterized in that: In step S1, a POTTER spray tower is used to evenly spray the test compound on the glass plate, and the test compound dosage is set to J, 1.8 mg / cm 2 ≤J≤2.2mg / cm 2 And wait for the compound to be tested to dry to obtain a dry reagent glass plate.

8. The method for preventing predatory mites from escaping based on toxicity quantitative testing according to claim 6, characterized in that: In the step S2, when cutting the rectangular groove and connecting the PVC plastic board with the nylon mesh, a fine brush knife is used to cut the rectangular groove on the PVC plastic board, and the nylon mesh is covered on the PVC plastic board to obtain a plastic board with a mesh, wherein the length of the rectangular groove is K2, and the width of the rectangular groove is L2, wherein K2=40 mm, L2=30 mm; In the step S3, a plastic plate with a net is covered on a glass plate to obtain a device for preventing predatory mites from escaping based on toxicity quantitative testing as described in the present scheme; In step S4, the predatory mite is placed in a paralysis device, and the paralysis time is set to t, t=2 min.

9. The method for preventing predatory mites from escaping based on toxicity quantitative testing according to claim 6, characterized in that: In step S5, a No. 0 wolf hair brush is used to pick a predatory mite at the center of the glass plate; In step S5, the device is placed in an artificial incubator, with the glass plate facing downward and the nylon mesh facing upward. The parameters of the artificial incubator are: the light-dark ratio is U, U=14h:10h, the incubation temperature is R, 24°C≤R≤26°C, and the incubation humidity is H, 75%≤H≤85%.

10. The method for preventing predatory mites from escaping based on toxicity quantitative testing according to claim 6, characterized in that: In step S6, when observing and recording the initial number of predatory mites in the rectangular slot and the number of predatory mites that died at each observation and recording time, and calculating the toxicity test result, the observation and recording time intervals are set to be G1, G2 and G3, the initial number of predatory mites is P0, the number of predatory mites that died at each observation and recording time is Pd1, Pd2 and Pd3, and the toxicity test result is X, where: G1=24h; G2=48h; G3=72h; X=[Pd1 / P0+(P0-Pd1) / (Pd2-Pd1)+(P0-Pd1-Pd2) / (Pd3-Pd2-Pd1)] / 3×100%.

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