Novel low-residue high-activity pesticide composition

Abstract

The invention belongs to the technical field of pesticide compositions, and particularly relates to a novel low-residue high-activity pesticide composition. According to the pesticide composition, the active ingredients of the pesticide composition are formed by compounding the trifluoropyridylamine and the lentinan, or formed by compounding the trifluoropyridylamine and the bacillus cereus; wherein the number of live spores in the bacillus cereus is 9 billion live spores per gram. The compound combination of the trifluoropyridylamine and the lentinan and the compound combination of the trifluoropyridylamine and the bacillus cereus show a synergistic interaction effect on crop diseases, so that compared with any single agent in the compound combination, the compound combination of the trifluoropyridylamine and the lentinan and the compound combination of the trifluoropyridylamine and the bacillus cereus can improve the prevention and treatment effect on the diseases, reduce the use dosage of pesticides and reduce the pesticide cost. Therefore, the pesticide composition disclosed by the invention has the characteristics of low residue and high activity.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide compositions, and in particular relates to a novel low-residue and high-activity pesticide composition. Background Art

[0002] Triflupyridine is a pesticide compound developed using "structure-based drug design technology". It has a novel chemical structure and contains both pyridineamide and cyclobutyl structures in the molecule. The specific structural formula is as follows:

[0003]

[0004] The common name of trifluoropyridinamine in English is: cyclobutrifluram, the CAS registration number is: 1460292-16-3, and the molecular formula is C 17 H 13 Cl2F3N2O, relative molecular mass: 389.20. Triflupyridamole is a succinate dehydrogenase inhibitor (SDHI) fungicide and nematicide. It interferes with the tricarboxylic acid cycle on the electron respiratory chain complex II, inhibits the function of mitochondria, prevents them from producing energy, inhibits the growth of pathogens, and eventually causes their death; in addition, triflupyridamole also inhibits the activity of succinate dehydrogenase, destroys the ATP production in the mitochondria of plant pathogenic nematodes, and prevents and controls a wide variety of nematodes such as tomato root-knot nematodes. In the agricultural production process, triflupyridamole is broad-spectrum and highly effective, and can provide lasting protection for crops from nematodes and soil-borne diseases that are difficult to detect but extremely destructive.

[0005] The main components of shiitake mushrooms are polysaccharides and fatty acids, and lentinan is an effective active ingredient extracted from high-quality shiitake mushroom fruiting bodies. It has the functions of anti-virus, regulating plant immune function and stimulating interferon formation. It effectively destroys viral genes and viral cells by inactivating viral activity and inhibiting viral replication. It has been disclosed in the prior art that when lentinan is compounded with active ingredients such as cyfluthrin, sulfoxaflor, cyfluthrin, flufenacet, chitosan oligosaccharide, fluopicolide or avermectin in a certain mass ratio, it shows a synergistic effect on nematodes.

[0006] Bacillus cereus is a microbial pesticide preparation with the characteristics of low toxicity, low residue and no environmental pollution. Bacillus cereus can be used to prevent and control diseases and pests on a variety of crops.

[0007] In agricultural production, long-term use of single-ingredient pesticides can easily lead to the development of pest resistance. As resistance accumulates, the control effect of pesticides will gradually decrease. Compounding the active ingredients of different pesticides is an effective and quick means to develop and research new pesticides and prevent and control resistant pests and diseases in agriculture. In the actual compounding process, the compound combination will show three types of effects: antagonism, additive effect and synergistic effect, but the specific type of action cannot be predicted and can only be determined through a large number of experiments.

[0008] The inventors have found through a large number of screening tests that the combination of trifluopyramide with Lentinan or Bacillus cereus has a synergistic effect, and there are no reports on related combinations. Summary of the invention

[0009] The purpose of the present invention is to provide a novel low-residue and high-activity pesticide composition to solve the problem that long-term use of single-component pesticides in agricultural production easily leads to the development of pest resistance, and as the resistance accumulates, the control effect of the pesticide will gradually decrease.

[0010] To achieve the above object, the present invention provides the following technical solutions:

[0011] A pesticide composition, wherein the active ingredient of the pesticide composition is compounded by trifluopyramide and lentinan, or trifluopyramide and Bacillus cereus; wherein the number of live spores in the Bacillus cereus is 9 billion live spores / gram.

[0012] Preferably, the mass ratio of trifluopyridine to lentinan is 1-100:100-1.

[0013] Furthermore, the mass ratio of trifluopyridine to lentinan is 1-7.5:15-1.

[0014] Preferably, the mass ratio of trifluopyramide to Bacillus cereus is 1-120:120-1.

[0015] Furthermore, the mass ratio of trifluopyridine to Bacillus cereus is 1:40-1.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The compound combination of trifluopyramide and Lentinan, and the compound combination of trifluopyramide and Bacillus cereus of the present invention show a synergistic effect on crop diseases. Compared with any single agent in the compound combination, the control effect of the disease can be improved, the dosage of pesticides can be reduced, and the pesticide residue and control cost can be reduced. In this way, the pesticide composition of the present invention has the characteristics of low residue and high activity.

[0018] (2) The action mechanism of trifluopyramide in the pesticide composition of the present invention is different from that of Lentinan or Bacillus cereus, which helps to reduce the risk of drug resistance of the pesticide composition of the present invention, delay the development of drug resistance, and extend the service life of the agent. DETAILED DESCRIPTION

[0019] Based on the embodiments of the present invention, all other embodiments obtained by technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0020] 1. Biological Activity Assay Example

[0021] Refer to "NT / Y 1833.1-2009 Guidelines for Indoor Bioassay Tests of Pesticides - Nematicides Part 1 - Tests for Inhibition of Plant Pathogenic Nematodes - Insect Immersion Method"

[0022] 1.1 Purpose of the experiment

[0023] Indoor toxicity screening of different compound combinations against the southern root-knot nematode (Meloidogyne incognita), the pathogen of tomato root-knot nematode disease

[0024] 1.2. Test Agents

[0025] 86% trifluopyramide parent drug (Syngenta Crop Protection Co., Ltd., Switzerland), 10% lentinan parent drug (Beijing Sanpu Baicao Biopharmaceutical Co., Ltd.), 9 billion live spores / gram of Bacillus cereus parent drug (Jiangxi Tianyou Biochemical Co., Ltd.)

[0026] Triflupyridamole was dissolved in dimethyl sulfoxide and then diluted with 0.1% Tween-80 aqueous solution to form a single-dose mother solution; Lentinan and Bacillus cereus (100% by mass) were directly prepared with 0.1% Tween-80 aqueous solution to form a single-dose mother solution. The single-dose mother solution and the compound combination were then diluted with 0.1% Tween-80 aqueous solution to form 6 mass concentration gradients. Based on a large number of mixing tests, the compound combinations in Tables 1 and 2 were tested for toxicity and displayed. The above agents are prepared and used immediately.

[0027] It should be noted that before compounding trifluopyramide with Bacillus cereus, the inventors conducted a compatibility test of trifluopyramide and Bacillus cereus. The results showed that at a low concentration (4 mg / L), trifluopyramide had a small inhibitory effect on the growth of Bacillus cereus. As the concentration increased, the inhibitory effect of trifluopyramide on the growth of Bacillus cereus gradually increased. When the concentration of trifluopyramide was 32 mg / L, the inhibitory effect was more obvious. The inhibitory effect of trifluopyramide and Bacillus cereus on southern root-knot nematodes was LC 50(1.203 mg / L and 83.746 mg / L respectively), trifluridine and Bacillus cereus can be compounded.

[0028] Table 1 Design of compound combination of trifluopyridine and Lentinan

[0029]

[0030] Table 2 Design of compound combination of trifluridine and Bacillus cereus

[0031]

[0032] 1.3 Test methods

[0033] Pick the root-knot nematode eggs on the roots of tomatoes, incubate them at 25°C indoors, take the second-instar larvae of the same age and resuspend them in sterile water to 120 heads / mL. Take 3mL of the drug solution and 3mL of the nematode suspension in a test tube and mix them evenly. Use a pipette to transfer 150μL of the above mixture into the small holes of a 24-well biological test plate, and use the treatment of 0.1% Tween-80 aqueous solution as a blank control. Place it in a 25°C constant temperature incubator for dark culture. After 24 hours, observe the survival of the root-knot nematodes under a stereo microscope. The judgment standard is that the nematodes are stiff and cannot bend and move when touched with a hairpin, which is considered dead. The number of dead nematodes and the total number are counted, and the mortality rate and corrected mortality rate of each treatment are calculated. Each treatment is repeated 3 times.

[0034] Mortality rate (%) = number of root-knot nematodes killed / total number of root-knot nematodes * 100%

[0035] Corrected mortality rate (%) = (treatment mortality rate - control mortality rate) / (1 - control mortality rate) * 100%

[0036] 1.4 Data Analysis

[0037] The LC of each treatment agent was calculated by regression analysis based on the logarithmic value of the agent and the corresponding nematode corrected mortality probability value. 50 The Sun Yunpei method was used to calculate the co-toxicity coefficient (CTC value) of the mixture, and the type of action of the compound agent was evaluated based on the calculated co-toxicity coefficient (CTC): CTC≤80 is antagonistic effect, 80<CTC<120 is additive effect, and CTC≥120 is synergistic effect. The results are shown in Table 3-4.

[0038] Table 3 Evaluation of the combined effects of trifluopyramide and Lentinan on southern root-knot nematodes

[0039]

[0040] It can be seen from Table 3 that the co-toxicity coefficients of the five combinations of trifluopyramide and Lentinan to southern root-knot nematodes are between 100.819 and 163.997. Among them, when the mass ratio of trifluopyramide to Lentinan is 7.5:1, 1:1, 1:7.5 and 1:15, the type of combined action is synergistic.

[0041] Table 4 Evaluation of the combined effects of trifluopyramide and Bacillus cereus on southern root-knot nematodes

[0042]

[0043] As shown in Table 4, the co-toxicity coefficients of the five combinations of trifluopyramide and Bacillus cereus to southern root-knot nematodes ranged from 74.705 to 383.973, and different ratios had different joint action modes on southern root-knot nematodes. Among them, when the mass ratio of trifluopyramide to Bacillus cereus was 1:1, 1:20 and 1:40, the joint action type was synergistic.

[0044] As can be seen from the above, the compound combination of trifluopyramide and Lentinan, and the compound combination of trifluopyramide and Bacillus cereus of the present invention show a synergistic effect on crop diseases. Compared with any single agent in the compound combination, the control effect of the disease can be improved, the dosage of pesticides can be reduced, and the pesticide residue and control cost can be reduced. In this way, the pesticide composition of the present invention has the characteristics of low residue and high activity.

Claims

1. A pesticide composition, characterized in that: The active ingredient of the pesticide composition is compounded by trifluopyramide and lentinan, or trifluopyramide and Bacillus cereus; wherein the number of live spores in the Bacillus cereus is 9 billion live spores / gram.

2. The pesticide composition according to claim 1, characterized in that The mass ratio of trifluopyridine to lentinan is 1-100:100-1.

3. The pesticide composition according to claim 2, characterized in that The mass ratio of trifluopyridine to lentinan is 1-7.5:15-1.

4. The pesticide composition according to claim 1, characterized in that The mass ratio of trifluopyridine to Bacillus cereus is 1-120:120-1.

5. The pesticide composition according to claim 1, characterized in that The mass ratio of trifluopyridine to Bacillus cereus is 1:40-1.

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