Agricultural composition for controlling mushroom flies and application thereof

Abstract

The invention discloses an agricultural composition for controlling mushroom flies and application of the agricultural composition, and belongs to the technical field of pesticides. The effective component of the agricultural composition is composed of a component A and a component B, the component A is rotenone or thiamethoxam, and the component B is a soybean trypsin inhibitor. In the agricultural composition, the mass ratio of the soybean trypsin inhibitor to the rotenone is (0.2-1): (0.3-3.5), or the mass ratio of the soybean trypsin inhibitor to the thiamethoxam is (0.2-1): (2.7-9.5), in the mass ratio range provided by the invention, the agricultural composition realizes advantage complementation among different insecticides, effectively improves the insecticidal effect, and also has a synergistic interaction effect. The agricultural composition provided by the invention can effectively reduce and delay the resistance development of pests, improve the pesticide effect and reduce environmental pollution.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides and relates to an agricultural composition for preventing and controlling mushroom flies and application thereof. Background Art

[0002] Mushroom flies are a general term for dipteran pests that harm edible fungi. They are characterized by many species, large numbers, and serious damage. The main cause of mushroom fly damage is feeding by larvae, followed by adults. Larvae harm culture media, mycelium and fruiting bodies, sucking nutrients from mycelium, causing mycelium to decline, and mushroom buds to die due to lack of nutrients. In addition, adults can also carry pathogens, nematodes or mites to harm edible fungi fruiting bodies, and are important media for the spread of diseases and pests in mushroom houses. Therefore, it is of great significance to develop a composition with better control effect on golden fruit flies.

[0003] Rotenone (Tubatoxin) is an isoflavone compound that exists in the root bark of leguminous plants. It has strong contact and stomach poisoning effects on insects, especially the larvae of the cabbage butterfly, the diamondback moth and aphids. Early studies have shown that rotenone can directly enter the animal body through the skin, stomata, etc., and mainly acts on the electron transfer system in the first part of the mitochondrial respiratory chain, inhibiting the reduced nicotinamide adenine dinucleotide (NADH), thereby blocking the respiration of cells in nerve and muscle tissues. In addition, rotenone can also inhibit the oxidation of L-glutamate, which inhibits nerve function and causes insect poisoning and paralysis. It has contact, stomach poisoning, antifeedant and fumigation effects on nearly 800 species of agricultural, forestry, storage and sanitary pests of the Hemiptera, Coleoptera, Thysanoptera, Lepidoptera, Hymenoptera and Orthoptera of the Insecta class.

[0004] Thiamethoxam belongs to the second generation of nicotinoid insecticides. Its insecticidal mechanism is: it can selectively inhibit the nicotinic acid acetylcholinesterase receptors in the insect central nervous system, thereby blocking the normal conduction of the insect central nervous system, causing the pests to be paralyzed and die. Thiamethoxam not only has contact, stomach poison, and systemic activity, but also has the characteristics of high efficiency, safety, long lasting effect, and fast action speed. It is highly active against Coleoptera, Diptera, Lepidoptera, and especially Hemiptera pests.

[0005] Soybean Trypsin Inhibitors are mainly found in soybean seeds and are serine protease inhibitors. Soybean Trypsin Inhibitors can inhibit the activity of digestive enzymes in pests, thereby affecting the growth, development and reproduction of pests. When soybean trypsin inhibitors enter the body of pests, they will bind to digestive enzymes, thereby reducing their activity or inactivating them, causing pests to be unable to effectively decompose protein in food, thereby affecting their growth, development and reproduction, manifested as slow growth, weight loss, and decreased reproduction rate of pests.

[0006] Long-term application of a single agent can easily lead to insecticide resistance in pests. Compounding agents with different mechanisms of action can delay or overcome the development of insecticide resistance in pests. Existing literature does not provide relevant records of compounding soybean trypsin inhibitor with rotenone or thiophanate-methyl to control mushroom flies. Therefore, it is of great significance to provide a compound agent that improves the control effect of mushroom flies by scientifically mixing soybean trypsin inhibitor with rotenone or thiophanate-methyl. Summary of the invention

[0007] In order to solve the above technical problems, the present invention provides an agricultural composition for controlling mushroom flies and its application. The active ingredients of the agricultural composition are composed of component A and component B, component A is rotenone or thiamethoxam, and component B is soybean trypsin inhibitor. Within the mass ratio range provided by the present invention, the agricultural composition achieves complementary advantages between different insecticides, effectively improves the insecticidal effect, and also has a synergistic effect. The agricultural composition provided by the present invention can effectively reduce and delay the development of pest resistance, improve drug efficacy, and reduce environmental pollution.

[0008] To achieve the technical purpose of the present invention, on the one hand, the present invention provides an agricultural composition for controlling mushroom flies, wherein the active ingredients of the agricultural composition consist of component A and component B, component A is rotenone or thiamethoxam, and component B is soybean trypsin inhibitor.

[0009] Furthermore, in the agricultural composition provided by the present invention, the mass ratio of soybean trypsin inhibitor to rotenone is 0.02-4.0:0.3-16.8; preferably, in the agricultural composition, the mass ratio of soybean trypsin inhibitor to rotenone is 0.2-1.0:0.3-3.5.

[0010] Specifically, the present invention found through indoor toxicity tests that after rotenone and soybean trypsin inhibitor were mixed in a mass ratio range of 0.3-3.5:0.2-1, the toxicity to golden fruit flies was significantly improved, and under this ratio condition, the co-toxicity coefficient of the agricultural composition was greater than 120, showing a synergistic effect. The control effect of rotenone alone on golden fruit flies was poor. After soybean trypsin inhibitor and rotenone were mixed in a mass ratio range of 0.2-1.0:0.3-3.5, the actual mortality rate was significantly higher than the predicted mortality rate.

[0011] Furthermore, in the agricultural composition provided by the present invention, the mass ratio of soybean trypsin inhibitor to thiamethoxam is 0.02-4.0:2.7-22.5; preferably, in the agricultural composition, the mass ratio of soybean trypsin inhibitor to thiamethoxam is 0.2-1.0:2.7-9.5.

[0012] Specifically, the present invention found through indoor toxicity tests that after thiamethoxam and soybean trypsin inhibitor were mixed in a mass ratio range of 2.7-9.5:0.2-1, the toxicity to golden fruit flies was significantly improved, and under this ratio condition, the co-toxicity coefficient of the agricultural composition was greater than 120, showing a synergistic effect. The control effect of thiamethoxam alone on golden fruit flies was poor. After soybean trypsin inhibitor and thiamethoxam were mixed, within the ratio range provided by the present invention, the actual mortality rate was significantly higher than the predicted mortality rate.

[0013] On the other hand, the present invention claims protection for the use of the above agricultural composition in controlling dipteran pests. The dipteran pests include mushroom flies, and the mushroom flies are golden fruit flies.

[0014] Furthermore, the agricultural composition inhibits the activity of pest proteases, and the proteases are total proteases and / or trypsin.

[0015] Specifically, the present invention detects the protease activity of pests and finds that soybean trypsin inhibitor can significantly reduce the total protease activity of golden fruit fly larvae, and the reducing effect increases with the increase of soybean trypsin inhibitor concentration, and the reducing effect of 1mg / L soybean trypsin inhibitor is stronger than that of 0.2mg / L soybean trypsin inhibitor. After rotenone and soybean trypsin inhibitor are compounded in a mass ratio of 0.3-3.5:0.2-1 or thiamethoxam and soybean trypsin inhibitor are compounded in a mass ratio of 2.7-9.5:0.2-1, the agricultural composition still has the effect of reducing the total protease activity of golden fruit fly larvae. Soybean trypsin inhibitor can significantly reduce the total protease activity of golden fruit fly larvae, and the reducing effect increases with the increase of soybean trypsin inhibitor concentration, and the reducing effect of 1mg / L soybean trypsin inhibitor is stronger than that of 0.2mg / L soybean trypsin inhibitor. After rotenone and soybean trypsin inhibitor are compounded in a mass ratio of 0.3-3.5:0.2-1, the agricultural composition still has the effect of reducing the total protease activity of golden fruit fly larvae.

[0016] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0017] (1) The agricultural composition provided by the present invention is composed of soybean trypsin inhibitor and rotenone, which has a better control effect on golden fruit flies. After the soybean trypsin inhibitor and rotenone are mixed in a mass ratio of 0.2-1:0.3-3.5, the control effect has a synergistic effect. The present invention found through indoor toxicity tests that after rotenone and soybean trypsin inhibitor are mixed in a mass ratio range of 0.3-3.5:0.2-1, the toxicity to golden fruit flies is significantly improved, and under this ratio condition, the co-toxicity coefficient of the agricultural composition is greater than 120, showing a synergistic effect. The control effect of rotenone alone on golden fruit flies is not good. After the soybean trypsin inhibitor and rotenone are mixed in a mass ratio range of 0.2-1.0:0.3-3.5, the actual mortality rate is significantly higher than the predicted mortality rate. It can be seen that after soybean trypsin inhibitor and rotenone are mixed in a mass ratio of 0.2-1.0:2.7-9.5, the synergistic effect of soybean trypsin inhibitor on the insecticidal effect of rotenone is very significant.

[0018] (2) The agricultural composition provided by the present invention is composed of soybean trypsin inhibitor and thiamethoxam, which has a better control effect on golden fruit flies. After the soybean trypsin inhibitor and thiamethoxam are mixed in a mass ratio of 0.2-1:0.3-3.5, the control effect has a synergistic effect. The present invention found through indoor toxicity tests that after thiamethoxam and soybean trypsin inhibitor are mixed in a mass ratio range of 2.7-9.5:0.2-1, the toxicity to golden fruit flies is significantly improved, and under this ratio condition, the co-toxicity coefficient of the agricultural composition is greater than 120, showing a synergistic effect. The control effect of thiamethoxam alone on golden fruit flies is not good. After the soybean trypsin inhibitor and thiamethoxam are mixed, the actual mortality rate is significantly higher than the predicted mortality rate within the ratio range provided by the present invention. It can be seen that after the soybean trypsin inhibitor and thiamethoxam are mixed in a mass ratio of 0.2-1.0:2.7-9.5, the synergistic effect of the soybean trypsin inhibitor on the insecticidal effect of rotenone is very significant.

[0019] (3) The agricultural composition of the present invention has the effect of inhibiting the activity of proteases in pests, and the proteases are total proteases and / or trypsin. The present invention has found through experiments that soybean trypsin inhibitor can significantly reduce the total protease activity of golden fruit fly larvae, and the reducing effect increases with the increase of soybean trypsin inhibitor concentration. The reducing effect of 1 mg / L soybean trypsin inhibitor is stronger than that of 0.2 mg / L soybean trypsin inhibitor. After rotenone and soybean trypsin inhibitor are compounded in a mass ratio of 0.3-3.5:0.2-1 or thiamethoxam and soybean trypsin inhibitor are compounded in a mass ratio of 2.7-9.5:0.2-1, the agricultural composition still has the effect of reducing the total protease activity of golden fruit fly larvae. Soybean trypsin inhibitor can extremely significantly reduce the total protease activity of golden fruit fly larvae, and the reducing effect increases with the increase of soybean trypsin inhibitor concentration. The reducing effect of 1 mg / L soybean trypsin inhibitor is stronger than that of 0.2 mg / L soybean trypsin inhibitor. After rotenone and soybean trypsin inhibitor are compounded in a mass ratio of 0.3-3.5:0.2-1 or thiamethoxam and soybean trypsin inhibitor are compounded in a mass ratio of 2.7-9.5:0.2-1, the agricultural composition still has the effect of reducing the total protease activity of golden fruit fly larvae. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention.

[0021] Figure 1 This is a statistical graph of the mortality rate of golden fruit flies after mixing different concentrations of rotenone and soybean trypsin inhibitor. Figure 1 A in the figure is the statistical result of the mortality of golden fruit flies after mixing different concentrations of rotenone and 0.2 mg / L soybean trypsin inhibitor; Figure 1 B in the figure is the statistical result of the mortality of golden fruit flies after mixing different concentrations of rotenone and 1 mg / L soybean trypsin inhibitor.

[0022] Figure 2 This is a statistical graph of the mortality rate of golden fruit flies after mixing different concentrations of thiamethoxam and soybean trypsin inhibitor. Figure 2 A in the figure is the statistical result of the mortality of golden fruit flies after mixing different concentrations of thiamethoxam and 0.2 mg / L soybean trypsin inhibitor; Figure 2 B in the figure is the statistical result of the mortality of golden fruit flies after mixing different concentrations of thiamethoxam and 1 mg / L soybean trypsin inhibitor.

[0023] Figure 3This is a graph showing the effect of the agricultural composition on the total protease activity of golden fruit fly larvae. Figure 3 A in the figure is the result of the effect of 0.2 mg / L soybean trypsin inhibitor in the agricultural composition on the total protease activity of golden fruit fly larvae; Figure 3 B is a graph showing the effect of 1 mg / L soybean trypsin inhibitor in the agricultural composition on the total protease activity of golden fruit fly larvae.

[0024] Figure 4 This is a graph showing the effect of the agricultural composition on the trypsin activity of golden fruit fly larvae. Figure 4 A in the figure is the effect of 0.2 mg / L soybean trypsin inhibitor in the agricultural composition on the trypsin activity of golden fruit fly larvae; Figure 4 B is a graph showing the effect of 1 mg / L soybean trypsin inhibitor in the agricultural composition on the trypsin activity of golden fruit fly larvae. DETAILED DESCRIPTION

[0025] The technical scheme of the present invention is described below in conjunction with the embodiments, but the present invention is not limited to the following embodiments. The experimental methods and detection methods described in each embodiment are conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0026] Soybean trypsin inhibitor was purchased from Suzhou Grease Biotechnology Co., Ltd., catalog number T6349;

[0027] Rotenone 6% microemulsion was purchased from Beijing Sanpu Baicao Green Plant Preparation Co., Ltd.;

[0028] Thiamethoxam 30% SC was purchased from Beijing Zhongbao Green Agriculture Technology Group Co., Ltd.

[0029] Example 1

[0030] This example provides an indoor toxicity test of an agricultural composition.

[0031] 1. Indoor toxicity test of agricultural composition with rotenone and soybean trypsin inhibitor as main active ingredients against golden fruit fly

[0032] Rotenone 6% microemulsion and soybean trypsin inhibitor (SBTI) were diluted with water as solvent. Rotenone 6% microemulsion and soybean trypsin inhibitor were prepared as a mixed agent or a single agent according to the effective ingredient ratio shown in Table 1. The apples were cut into 5cm×5cm×1cm slices and soaked in the agent for 30s. After taking out, the excess liquid on the surface was sucked off and placed in a disposable culture dish. 12 golden fruit fly 3rd instar larvae were inoculated in each dish for bioassay, and three replicates were set for each treatment. The toxicity regression curve of rotenone 6% microemulsion to golden fruit fly 3rd instar larvae obtained by bioassay was: y=0.786x-1.711, and the correlation coefficient was 0.91. The co-toxicity coefficient method was used to evaluate the synergistic effect of the agricultural composition, establish the toxicity regression equation, and calculate the LC of each agent. 50 The Sun-Johnson method was used to calculate the co-toxicity coefficient (CTC value) of each compound mixture. Soybean trypsin inhibitor was used as the standard agent, and its toxicity index (TI) was 100. The calculation results are shown in Table 1.

[0033]

[0034] TTI = TI of agent A × the percentage of agent A in the mixture + TI of agent B × the percentage of agent B in the mixture;

[0035]

[0036] In the formula, TI is the toxicity index of the measured single agent, ATI is the toxicity index of the measured mixture, TTI is the toxicity index of the theoretical mixture, CTC is the co-toxicity coefficient of the compound, A is the standard agent soybean trypsin inhibitor, and B is rotenone. The synergistic effect of the mixture is judged by the CTC value, CTC value ≥ 120 is synergistic effect, CTC value ≤ 80 is antagonistic effect, and 80 < CTC value < 120 is additive effect.

[0037] Table 1: Results of the synergistic effect of the agricultural composition on the toxicity of golden fruit flies

[0038] Pharmacy Quality ratio <![CDATA[LC 50 / (mg / L)]]> TI ATI TTI CTC SBTI / 1.11 100 / / / Rotenone / 168.49 0.66 / / / Rotenone:SBTI 0.3:0.2 0.61 / 181.97 40.4 450.42 Rotenone:SBTI 1.3:0.2 1.15 / 96.52 13.91 693.89 Rotenone:SBTI 3.5:0.2 2.83 / 39.22 6.03 650.41 Rotenone:SBTI 0.3:1 1.09 / 101.83 77.08 132.11 Rotenone:SBTI 1.3:1 1.99 / 55.78 43.85 127.21 Rotenone:SBTI 3.5:1 3.81 / 29.13 22.74 128.10 Rotenone:SBTI 0.3:2 1.15 / 96.52 87.04 110.89 Rotenone:SBTI 1.3:2 1.71 / 64.91 60.87 106.64 Rotenone:SBTI 3.5:2 2.96 / 37.50 36.78 101.96 Rotenone:SBTI 0.3:4 1.05 / 105.71 93.07 113.58 Rotenone:SBTI 1.3:4 1.45 / 76.55 75.63 101.22 Rotenone:SBTI 3.5:4 2.11 / 52.61 53.64 98.08

[0039] As shown in Table 1, after rotenone and soybean trypsin inhibitor were mixed in a mass ratio range of 0.3-3.5:0.2-1, the toxicity to golden fruit flies was significantly improved, and under this ratio condition, the co-toxicity coefficient of the agricultural composition was greater than 120, showing a synergistic effect.

[0040] The corrected mortality and predicted mortality of each agent on golden fruit flies were calculated. The predicted mortality and actual mortality were subjected to Student t analysis of variance. If the actual mortality was significantly higher than the predicted mortality, it indicated a synergistic effect (P<0.05), if there was no significant difference, it indicated no synergistic effect (P≥0.05), and if the actual mortality was significantly lower than the predicted mortality, it indicated an antagonistic effect (P<0.05). The calculation results are shown in Table 2. The statistical results of the mortality of golden fruit flies after the combination of rotenone and soybean trypsin inhibitor are shown in Table 2. Figure 1 shown.

[0041] Corrected mortality rate (%) = (mortality rate of treatment group - mortality rate of control group) / (1-mortality rate of control group) × 100%;

[0042] Survival rate S (%) = 1-corrected mortality rate;

[0043] Predicted mortality rate = [1-S a ×S b ]×100%.

[0044] In the formula, a is rotenone and b is soybean trypsin inhibitor.

[0045] Table 2: Results of indoor toxicity test of agricultural compositions against golden fruit flies

[0046]

[0047]

[0048] As shown in Table 2, the control effect of rotenone alone on golden fruit flies is not good. After soybean trypsin inhibitor and rotenone are mixed in a mass ratio of 0.2-1.0:0.3-3.5, the actual mortality rate is significantly higher than the predicted mortality rate. It can be seen that after soybean trypsin inhibitor and rotenone are mixed in a mass ratio of 0.2-1:0.3-3.5, the synergistic effect of soybean trypsin inhibitor on the insecticidal effect of rotenone is very significant.

[0049] 2. Indoor toxicity test of agricultural composition with thiamethoxam and soybean trypsin inhibitor as main active ingredients against golden fruit flies

[0050] Thiamethoxam 30% suspension and soybean trypsin inhibitor (SBTI) were diluted with water as solvent. Thiamethoxam 30% suspension and soybean trypsin inhibitor were prepared as a mixed agent or a single agent according to the effective ingredient ratio shown in Table 3. The apples were cut into 5cm×5cm×1cm slices and soaked in the agent for 30s. After taking out, the excess liquid on the surface was sucked off and placed in a disposable culture dish. Twelve golden fruit fly third-instar larvae were inoculated in each dish for bioassay, and three replicates were set for each treatment. The toxicity regression curve of thiamethoxam 30% suspension to golden fruit fly third-instar larvae obtained by bioassay was: y=1.4x-3.294, and the correlation coefficient was 0.981. The co-toxicity coefficient method was used to evaluate the synergistic effect of the agricultural composition, establish the toxicity regression equation, and calculate the LC of each agent. 50 The Sun-Johnson method was used to calculate the co-toxicity coefficient (CTC value) of each compound mixture. Soybean trypsin inhibitor was used as the standard agent, and its toxicity index (TI) was 100. The calculation results are shown in Table 3.

[0051] Table 3: Results of the synergistic effect of the agricultural composition on the toxicity of golden fruit flies

[0052] Pharmacy Quality ratio <![CDATA[LC 50 / (mg / L)]]> TI ATI TTI CTC SBTI / 1.11 100 / / / Thiamethoxam / 225.58 0.49 / / / Thiamethoxam: SBTI 2.7:0.2 4.37 / 25.40 7.35 345.58 Thiamethoxam: SBTI 5.6:0.2 5.52 / 20.11 3.92 513.01 Thiamethoxam: SBTI 9.5:0.2 8.23 / 13.49 2.54 531.10 Thiamethoxam: SBTI 2.7:1 3.02 / 36.75 27.38 134.22 Thiamethoxam: SBTI 5.6:1 5.34 / 20.79 15.57 133.53 Thiamethoxam: SBTI 9.5:1 8.91 / 12.46 9.97 124.97 Thiamethoxam: SBTI 2.7:2 2.23 / 49.78 42.83 116.23 Thiamethoxam: SBTI 5.6:2 3.91 / 28.39 26.68 106.41 Thiamethoxam: SBTI 9.5:2 5.38 / 20.63 17.8 115.90 Thiamethoxam: SBTI 2.7:4 1.68 / 66.07 59.9 110.30 Thiamethoxam: SBTI 5.6:4 2.43 / 45.68 41.95 108.89 Thiamethoxam: SBTI 9.5:4 3.54 / 31.36 29.97 104.64

[0053] As shown in Table 3, after thiamethoxam and soybean trypsin inhibitor were mixed in a mass ratio range of 2.7-9.5:0.2-1, the toxicity to golden fruit flies was significantly improved, and under this ratio condition, the co-toxicity coefficient of the agricultural composition was greater than 120, showing a synergistic effect.

[0054] The corrected mortality and predicted mortality of each agent on golden fruit flies were calculated. The predicted mortality and actual mortality were subjected to Student t analysis of variance. If the actual mortality was significantly higher than the predicted mortality, it indicated a synergistic effect (P<0.05), if there was no significant difference, it indicated no synergistic effect (P≥0.05), and if the actual mortality was significantly lower than the predicted mortality, it indicated an antagonistic effect (P<0.05). The calculation results are shown in Table 4. The statistical results of the mortality of golden fruit flies after the combination of thiamethoxam and soybean trypsin inhibitor are shown in Table 4. Figure 2 shown.

[0055] Table 4: Results of indoor toxicity test of agricultural compositions against golden fruit flies

[0056]

[0057] As shown in Table 4, the control effect of thiamethoxam alone on golden fruit flies is not good. After soybean trypsin inhibitor and thiamethoxam are mixed, the actual mortality rate is significantly higher than the predicted mortality rate within the ratio range provided by the present invention. It can be seen that after soybean trypsin inhibitor and thiamethoxam are mixed in a mass ratio of 0.2-1.0:2.7-9.5, the synergistic effect of soybean trypsin inhibitor on the insecticidal effect of rotenone is very significant.

[0058] Example 2

[0059] This example provides the effects of the agricultural composition on the enzymatic activities of two important proteases of the third instar larvae of Drosophila aurantifolia.

[0060] Cut the apple into 5cm×5cm×1cm slices and soak them in the agent for 30s. After taking them out, remove the excess liquid on the surface and put them in a disposable culture dish. Inoculate 12 golden fruit fly 3rd instar larvae in each dish for bioassay. Set up three replicates for each treatment. After grinding the insect body on ice, centrifuge and take the supernatant as tissue fluid for enzyme activity detection. Detect the total protease activity and trypsin activity respectively. The test results are as follows: Figure 3 and Figure 4 shown.

[0061] Depend on Figure 3 It can be seen that soybean trypsin inhibitor can significantly reduce the total protease activity of golden fruit fly larvae, and the reducing effect increases with the increase of soybean trypsin inhibitor concentration. The reducing effect of 1 mg / L soybean trypsin inhibitor is stronger than that of 0.2 mg / L soybean trypsin inhibitor. After rotenone and soybean trypsin inhibitor are compounded in a mass ratio of 0.3-3.5:0.2-1 or thiamethoxam and soybean trypsin inhibitor are compounded in a mass ratio of 2.7-9.5:0.2-1, the agricultural composition still has the effect of reducing the total protease activity of golden fruit fly larvae.

[0062] Depend on Figure 4 It can be seen that soybean trypsin inhibitor can significantly reduce the total protease activity of golden fruit fly larvae, and the reducing effect increases with the increase of soybean trypsin inhibitor concentration. The reducing effect of 1 mg / L soybean trypsin inhibitor is stronger than that of 0.2 mg / L soybean trypsin inhibitor. After rotenone and soybean trypsin inhibitor are compounded in a mass ratio of 0.3-3.5:0.2-1, the agricultural composition still has the effect of reducing the total protease activity of golden fruit fly larvae; after thiamethoxam and soybean trypsin inhibitor are compounded in a mass ratio of 2.7-9.5:0.2-1 or thiamethoxam and soybean trypsin inhibitor are compounded in a mass ratio of 2.7-9.5:0.2-1, the agricultural composition still has the effect of reducing the total protease activity of golden fruit fly larvae.

[0063] The embodiments described above are part of the embodiments of the present invention, but not all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but only represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.

Claims

1. An agricultural composition for controlling mushroom flies, characterized in that: The active ingredients of the agricultural composition are composed of component A and component B; The component A is rotenone or thiamethoxam; The component B is soybean trypsin inhibitor.

2. The agricultural composition according to claim 1, characterized in that When the component A is rotenone, the mass ratio of soybean trypsin inhibitor to rotenone in the agricultural composition is 0.2-1.0:0.3-3.

5.

3. The agricultural composition according to claim 1, characterized in that When the component A is thiamethoxam, the mass ratio of soybean trypsin inhibitor to thiamethoxam in the agricultural composition is 0.2-1.0:2.7-9.

5.

4. Use of the agricultural composition according to any one of claims 1 to 3 in controlling dipteran pests.

5. The use according to claim 4, characterized in that: The dipteran pests include mushroom flies, and the mushroom flies are golden fruit flies.

6. The use according to claim 4, characterized in that: The agricultural composition inhibits the activity of pest proteases.

7. The use according to claim 6, characterized in that: The protease is total protease and / or trypsin.

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